BRPI0516619A - reagentes e métodos para reprodutibilidade aperfeiçoada e redução de iniciação errÈnia em amplificação por pcr - Google Patents

reagentes e métodos para reprodutibilidade aperfeiçoada e redução de iniciação errÈnia em amplificação por pcr

Info

Publication number
BRPI0516619A
BRPI0516619A BRPI0516619-5A BRPI0516619A BRPI0516619A BR PI0516619 A BRPI0516619 A BR PI0516619A BR PI0516619 A BRPI0516619 A BR PI0516619A BR PI0516619 A BRPI0516619 A BR PI0516619A
Authority
BR
Brazil
Prior art keywords
reagents
pcr amplification
reduction
methods
pcr
Prior art date
Application number
BRPI0516619-5A
Other languages
English (en)
Inventor
Lawrence J Wangh
John Rice
Aquiles J Sanchez
Kenneth Pierce
Jesse Salk
Arthur Reis
Original Assignee
Univ Brandeis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Brandeis filed Critical Univ Brandeis
Publication of BRPI0516619A publication Critical patent/BRPI0516619A/pt
Publication of BRPI0516619B1 publication Critical patent/BRPI0516619B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/107—Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

REAGENTES E MéTODOS PARA REPRODUTIBILIDADE APERFEIçOADA E REDUçãO DE INICIAçãO ERRÈNEA EM AMPLIFICAçãO POR PCR. Um aditivo para prevenção de iniciação errónea em amplificações por reação em cadeia de polimerase (PCR) e ensaios compreendendo um oligonucleotídeo de hairpin tendo uma dupla de tronco maior do que seis nucleotídeos de comprimento e um término de tronco estabilizado. O aditivo melhora as amplificações por PCR, incluindo amplificações por LATE-PCR, quando adicionado às misturas de reação de amplificação iniciais. Ele pode ser incluído em conjuntos de oligonucleotídeo e em kits para amplificação e ensaios por PCR.
BRPI0516619A 2004-10-18 2005-10-17 reagentes, kit de reagentes e conjunto de oligonucleotídeos para realização de uma amplificação por reação em cadeia de polimerase BRPI0516619B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61967004P 2004-10-18 2004-10-18
PCT/US2005/037668 WO2006044995A1 (en) 2004-10-18 2005-10-17 Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification

Publications (2)

Publication Number Publication Date
BRPI0516619A true BRPI0516619A (pt) 2008-09-16
BRPI0516619B1 BRPI0516619B1 (pt) 2016-02-10

Family

ID=36203307

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0516619A BRPI0516619B1 (pt) 2004-10-18 2005-10-17 reagentes, kit de reagentes e conjunto de oligonucleotídeos para realização de uma amplificação por reação em cadeia de polimerase

Country Status (14)

Country Link
US (2) US7517977B2 (pt)
EP (1) EP1802774B1 (pt)
JP (2) JP5165376B2 (pt)
KR (1) KR20070090158A (pt)
CN (1) CN101076608B (pt)
AT (1) ATE541055T1 (pt)
AU (1) AU2005295299B2 (pt)
BR (1) BRPI0516619B1 (pt)
CA (1) CA2584576C (pt)
ES (1) ES2380775T3 (pt)
NO (1) NO341716B1 (pt)
NZ (1) NZ554701A (pt)
RU (1) RU2495046C2 (pt)
WO (1) WO2006044995A1 (pt)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ554701A (en) * 2004-10-18 2010-03-26 Univ Brandeis Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
EP1943348B1 (en) * 2005-10-03 2013-01-02 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US7972786B2 (en) * 2006-07-07 2011-07-05 Brandeis University Detection and analysis of influenza virus
US9034605B2 (en) * 2009-03-12 2015-05-19 Brandeis University Reagents and methods for PCR
PT2412718T (pt) * 2009-03-26 2016-12-30 Xiamen Amoy Diagnostics Co Ltd Iniciador em forma de anel utilizado na amplificação de ácido nucleico e a sua utilização
US8815514B2 (en) * 2009-07-02 2014-08-26 Co-Diagnostics, Inc. Detection primers for nucleic acid extension or amplification reactions
ES2743959T3 (es) 2009-10-21 2020-02-21 Univ Brandeis Kits para analizar secuencias de ácido nucleico monocatenario
US9169514B2 (en) * 2010-12-03 2015-10-27 Brandeis University Detecting nucleic acid variations within populations of genomes
JP6490576B2 (ja) * 2012-04-12 2019-03-27 ニユー・イングランド・バイオレイブス・インコーポレイテツド 重合反応のための合成核酸
WO2014116860A1 (en) * 2013-01-23 2014-07-31 Brandeis University Reagents for improving pcr accuracy
WO2015013521A1 (en) * 2013-07-24 2015-01-29 Brandeis University Dna methylation analysis
WO2015038869A1 (en) * 2013-09-13 2015-03-19 Brandeis University Modified polymerase compositions, methods and kits
CN114032234A (zh) * 2014-05-19 2022-02-11 威廉马歇莱思大学 使用重叠非等位基因特异性引物和等位基因特异性阻断剂寡核苷酸进行等位基因特异性扩增
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
US10240178B2 (en) 2014-12-19 2019-03-26 Brandeis University Mispriming prevention reagents
US10557134B2 (en) 2015-02-24 2020-02-11 Trustees Of Boston University Protection of barcodes during DNA amplification using molecular hairpins
EP3458613B1 (en) * 2016-05-18 2022-09-14 Integrated Nano-Technologies, Inc. Method for detection of a pcr product
AU2017330304A1 (en) 2016-09-23 2019-04-11 Alveo Technologies, Inc. Methods and compositions for detecting analytes
MX2021007311A (es) * 2018-12-20 2021-09-21 Alveo Tech Inc Metodos y composiciones para reducir la amplificacion no especifica en reacciones de amplificacion isotermica.
AU2019401601B2 (en) 2018-12-20 2026-04-02 Alveo Technologies, Inc. Handheld impedance-based diagnostic test system for detecting analytes
KR102275038B1 (ko) * 2019-12-11 2021-07-08 (주) 제노텍 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트
EP4169931A4 (en) * 2020-06-22 2025-05-07 Nuhigh Biotechnologies Co., Ltd Nucleic acid ligand and its use
US12472492B2 (en) 2020-08-14 2025-11-18 Alveo Technologies, Inc. Systems and methods of sample depositing and testing

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5874557A (en) * 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5693502A (en) * 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5811300A (en) * 1992-12-07 1998-09-22 Ribozyme Pharmaceuticals, Inc. TNF-α ribozymes
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US6183967B1 (en) * 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
CA2223078C (en) 1995-06-07 2008-11-25 Gilead Sciences, Inc. Nucleic acid ligands that bind to and inhibit dna polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6787308B2 (en) * 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
WO2001002559A1 (en) * 1999-07-02 2001-01-11 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
EP1353936A4 (en) * 2000-12-27 2005-05-18 Invitrogen Corp PRIMER AND METHOD FOR THE DETECTION AND DISTINCTION OF NUCLEIC ACIDS
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
WO2004090153A2 (en) * 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
NZ554701A (en) * 2004-10-18 2010-03-26 Univ Brandeis Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
EP1943348B1 (en) * 2005-10-03 2013-01-02 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids

Also Published As

Publication number Publication date
NO20072507L (no) 2007-07-06
NZ554701A (en) 2010-03-26
RU2495046C2 (ru) 2013-10-10
JP2008516613A (ja) 2008-05-22
US20060177842A1 (en) 2006-08-10
AU2005295299B2 (en) 2010-10-21
CN101076608B (zh) 2013-04-17
CA2584576A1 (en) 2006-04-27
AU2005295299A1 (en) 2006-04-27
US20090226973A1 (en) 2009-09-10
KR20070090158A (ko) 2007-09-05
WO2006044995A1 (en) 2006-04-27
BRPI0516619B1 (pt) 2016-02-10
EP1802774A4 (en) 2008-10-01
CN101076608A (zh) 2007-11-21
JP5165376B2 (ja) 2013-03-21
JP2013063070A (ja) 2013-04-11
EP1802774A1 (en) 2007-07-04
ES2380775T3 (es) 2012-05-18
EP1802774B1 (en) 2012-01-11
CA2584576C (en) 2015-12-08
US7517977B2 (en) 2009-04-14
NO341716B1 (no) 2018-01-08
RU2007118546A (ru) 2008-11-27
ATE541055T1 (de) 2012-01-15

Similar Documents

Publication Publication Date Title
NO20072507L (no) Reagenser og fremgangsmater for a bedre reproduserbarhet og redusere feil priming i PCR-amplifikasjon
WO2008034110A3 (en) Use of taq polymerase mutant enzymes for dna amplification in the presence of pcr inhibitors
WO2008149346A3 (en) Enhanced sensitivity polymerase chain reactions
WO2005083068A3 (en) Methods for obtaining thermostable enzymes, dna polymerase i variants from thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications to the same
WO2008033442A3 (en) Methods and compositions for performing low background multiplex nucleic acid amplification reactions
WO2009012246A3 (en) Nicking and extension amplification reaction for the exponential amplification of nucleic acids
EP2098592A3 (de) RNA-abhängige RNA-Polymerase, Verfahren und Kits zur Amplifikation und/oder Markierung von RNA
ES2570828T3 (es) Método de análisis de metilación
ATE550441T1 (de) Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
WO2007118147A3 (en) Competitive oligonucleotides
ATE532878T1 (de) Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas
WO2001096612A3 (en) Method of identifying and quantifying specific fungi and bacteria
EP2044191A4 (en) SYSTEMS AND METHOD FOR MONITORING THE AMPLIFICATION AND DISSOCIATION BEHAVIOR OF DNA MOLECULES
PL2210954T3 (pl) Określanie stopnia metylacji DNA
DK1438437T3 (da) Fremgangsmåde til påvisning af cytosin-methyleringer i immobiliserede DNA-pröver
Gouveia et al. Direct amplification of reference samples with Globalfiler® PCR Amplification Kit
UA99793C2 (ru) Набор для определения вируса гепатита в
WO2006030455A8 (en) Dna polymerases having strand displacement activity
DE60331072D1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität
ATE464397T1 (de) Cpg-amplicon und array-protokoll
WO2011104694A3 (en) Detection of braf v600e mutation by allele specific real time quantitative pcr (as-qpcr) using locked nucleic acids primers and beacon probes
WO2008149855A1 (ja) メチル化核酸又は非メチル化核酸を増幅する方法
WO2008093336A3 (en) Genome determination assay
GB0621361D0 (en) Use of DNA polymerases
WO2006119419A3 (en) Materials and kits for use in hot-start pcr, and methods of amplifying nucleic acids in a polymerase chain reaction

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 10/02/2016, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time
B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2433 DE 22-08-2017 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.