BRPI0516619A - reagentes e métodos para reprodutibilidade aperfeiçoada e redução de iniciação errÈnia em amplificação por pcr - Google Patents

reagentes e métodos para reprodutibilidade aperfeiçoada e redução de iniciação errÈnia em amplificação por pcr

Info

Publication number
BRPI0516619A
BRPI0516619A BRPI0516619-5A BRPI0516619A BRPI0516619A BR PI0516619 A BRPI0516619 A BR PI0516619A BR PI0516619 A BRPI0516619 A BR PI0516619A BR PI0516619 A BRPI0516619 A BR PI0516619A
Authority
BR
Brazil
Prior art keywords
reagents
pcr amplification
reduction
methods
pcr
Prior art date
Application number
BRPI0516619-5A
Other languages
English (en)
Inventor
Lawrence J Wangh
John Rice
Aquiles J Sanchez
Kenneth Pierce
Jesse Salk
Arthur Reis
Original Assignee
Univ Brandeis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Brandeis filed Critical Univ Brandeis
Publication of BRPI0516619A publication Critical patent/BRPI0516619A/pt
Publication of BRPI0516619B1 publication Critical patent/BRPI0516619B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/107—Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

REAGENTES E MéTODOS PARA REPRODUTIBILIDADE APERFEIçOADA E REDUçãO DE INICIAçãO ERRÈNEA EM AMPLIFICAçãO POR PCR. Um aditivo para prevenção de iniciação errónea em amplificações por reação em cadeia de polimerase (PCR) e ensaios compreendendo um oligonucleotídeo de hairpin tendo uma dupla de tronco maior do que seis nucleotídeos de comprimento e um término de tronco estabilizado. O aditivo melhora as amplificações por PCR, incluindo amplificações por LATE-PCR, quando adicionado às misturas de reação de amplificação iniciais. Ele pode ser incluído em conjuntos de oligonucleotídeo e em kits para amplificação e ensaios por PCR.
BRPI0516619A 2004-10-18 2005-10-17 reagentes, kit de reagentes e conjunto de oligonucleotídeos para realização de uma amplificação por reação em cadeia de polimerase BRPI0516619B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61967004P 2004-10-18 2004-10-18
PCT/US2005/037668 WO2006044995A1 (en) 2004-10-18 2005-10-17 Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification

Publications (2)

Publication Number Publication Date
BRPI0516619A true BRPI0516619A (pt) 2008-09-16
BRPI0516619B1 BRPI0516619B1 (pt) 2016-02-10

Family

ID=36203307

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0516619A BRPI0516619B1 (pt) 2004-10-18 2005-10-17 reagentes, kit de reagentes e conjunto de oligonucleotídeos para realização de uma amplificação por reação em cadeia de polimerase

Country Status (14)

Country Link
US (2) US7517977B2 (pt)
EP (1) EP1802774B1 (pt)
JP (2) JP5165376B2 (pt)
KR (1) KR20070090158A (pt)
CN (1) CN101076608B (pt)
AT (1) ATE541055T1 (pt)
AU (1) AU2005295299B2 (pt)
BR (1) BRPI0516619B1 (pt)
CA (1) CA2584576C (pt)
ES (1) ES2380775T3 (pt)
NO (1) NO341716B1 (pt)
NZ (1) NZ554701A (pt)
RU (1) RU2495046C2 (pt)
WO (1) WO2006044995A1 (pt)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE541055T1 (de) * 2004-10-18 2012-01-15 Univ Brandeis Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation
US20070212704A1 (en) * 2005-10-03 2007-09-13 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US7972786B2 (en) * 2006-07-07 2011-07-05 Brandeis University Detection and analysis of influenza virus
AU2010224100B2 (en) * 2009-03-12 2015-10-22 Brandeis University Reagents and methods for PCR
JP5818018B2 (ja) 2009-03-26 2015-11-18 厦門艾徳生物医薬科技有限公司 核酸増幅を用いる環状プライマー及びその応用
US8815514B2 (en) * 2009-07-02 2014-08-26 Co-Diagnostics, Inc. Detection primers for nucleic acid extension or amplification reactions
ES2743959T3 (es) 2009-10-21 2020-02-21 Univ Brandeis Kits para analizar secuencias de ácido nucleico monocatenario
CN103459612A (zh) 2010-12-03 2013-12-18 布兰代斯大学 用于检测野生型群体中的核酸突变体的方法和试剂盒
WO2013154898A1 (en) * 2012-04-12 2013-10-17 Ong, Jennifer Synthetic nucleic acids for polymerization reactions
US10301688B2 (en) * 2013-01-23 2019-05-28 Brandeis University Reagents for improving PCR accuracy
EP3024946A1 (en) * 2013-07-24 2016-06-01 Brandeis University Dna methylation analysis
WO2015038869A1 (en) * 2013-09-13 2015-03-19 Brandeis University Modified polymerase compositions, methods and kits
WO2015179339A1 (en) * 2014-05-19 2015-11-26 William Marsh Rice University Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
CA2971200A1 (en) 2014-12-19 2016-06-23 Brandeis University Mispriming prevention reagents
US10557134B2 (en) 2015-02-24 2020-02-11 Trustees Of Boston University Protection of barcodes during DNA amplification using molecular hairpins
EP3458613B1 (en) * 2016-05-18 2022-09-14 Integrated Nano-Technologies, Inc. Method for detection of a pcr product
CN109996888A (zh) 2016-09-23 2019-07-09 阿尔韦奥科技公司 用于检测分析物的方法和组合物
EP3899022A4 (en) 2018-12-20 2023-03-01 Alveo Technologies Inc. PORTABLE IMPEDANCE-BASED DIAGNOSTIC TEST SYSTEM FOR DETECTING ANALYTES
MX2021007311A (es) * 2018-12-20 2021-09-21 Alveo Tech Inc Metodos y composiciones para reducir la amplificacion no especifica en reacciones de amplificacion isotermica.
KR102275038B1 (ko) * 2019-12-11 2021-07-08 (주) 제노텍 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트
JP2023531027A (ja) * 2020-06-22 2023-07-20 ▲蘇▼州新海生物科技股▲ふん▼有限公司 核酸リガンド、およびその使用
US12472492B2 (en) 2020-08-14 2025-11-18 Alveo Technologies, Inc. Systems and methods of sample depositing and testing

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US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
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Also Published As

Publication number Publication date
NO20072507L (no) 2007-07-06
AU2005295299A1 (en) 2006-04-27
EP1802774A4 (en) 2008-10-01
RU2007118546A (ru) 2008-11-27
US7517977B2 (en) 2009-04-14
ES2380775T3 (es) 2012-05-18
EP1802774A1 (en) 2007-07-04
ATE541055T1 (de) 2012-01-15
EP1802774B1 (en) 2012-01-11
KR20070090158A (ko) 2007-09-05
CN101076608A (zh) 2007-11-21
AU2005295299B2 (en) 2010-10-21
CA2584576C (en) 2015-12-08
NO341716B1 (no) 2018-01-08
CA2584576A1 (en) 2006-04-27
JP5165376B2 (ja) 2013-03-21
US20060177842A1 (en) 2006-08-10
JP2013063070A (ja) 2013-04-11
RU2495046C2 (ru) 2013-10-10
US20090226973A1 (en) 2009-09-10
NZ554701A (en) 2010-03-26
BRPI0516619B1 (pt) 2016-02-10
JP2008516613A (ja) 2008-05-22
CN101076608B (zh) 2013-04-17
WO2006044995A1 (en) 2006-04-27

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 10/02/2016, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time
B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2433 DE 22-08-2017 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.