BRPI0609044A2 - método e kit de ensaio para a determinação de atividade de timidina cinase em uma amostra biológica, uso de método e/ou de kit de ensaio, método para uso em diagnóstico, monitoração e/ou prognóstico de doenças ou distúrbios de proliferação celular, e método para uso em triagem de compostos - Google Patents
método e kit de ensaio para a determinação de atividade de timidina cinase em uma amostra biológica, uso de método e/ou de kit de ensaio, método para uso em diagnóstico, monitoração e/ou prognóstico de doenças ou distúrbios de proliferação celular, e método para uso em triagem de compostos Download PDFInfo
- Publication number
- BRPI0609044A2 BRPI0609044A2 BRPI0609044-3A BRPI0609044A BRPI0609044A2 BR PI0609044 A2 BRPI0609044 A2 BR PI0609044A2 BR PI0609044 A BRPI0609044 A BR PI0609044A BR PI0609044 A2 BRPI0609044 A2 BR PI0609044A2
- Authority
- BR
- Brazil
- Prior art keywords
- kit
- biological sample
- disorders
- assay method
- determination
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
MéTODO E KIT DE ENSAIO PARA A DETERMINAçãO DE ATIVIDADE DE TIMIDINA CINASE EM UMA AMOSTRA BIOLóGICA, USO DE MéTODO E/OU DE KIT DE ENSAIO, MéTODO PARA USO EM DIAGNóSTICO, MONITORAçãO E/OU PROGNóSTICO DE DOENçAS OU DISTúRBIOS DE PROLIFERAçãO CELULAR, E, MéTODO PARA USO EM TRIAGEM DE COMPOSTOS. São descritos um método e um kit de ensaio para a determinação da atividade de timidina cinase (TK) em uma amostra biológica, tal como sangue, soro, plasma, Fluido Cérebro-Espinhal (CSF), fluido pleural, ascite, tecidos, células e seus extratos. O método compreende contatar com a amostra biológica, em um tampão, uma Mistura Reacional Básica compreendendo: gabarito e/ou iniciador ligado em superficie sólida, um desoxinucleosídeo modificado, tal como Bromo-desóxiUridina, Iodo- desóxiUridina, Fluoro-desóxiUridina ou Vinil-desóxiUridina como um substrato enzima cinase, um doador de fosfato, uma enzima polimerizadora de nucleosídeo, e uma fonte de enzima destituída de atividade de TK, tal como um extrato de levedura. Após a incubação é determinada a quantidade de desoxinucleosídeo modificado que tem sido incorporado no gabarito e/ou iniciador ligado em superficie sólida, e a atividade de TK presente na amostra biológica é diretamente proporcional à quantidade de desoxinucleosídeo modificado incorporado. O método e o kit de ensaio são úteis em diagnóstico, prognóstico, monitoração de progressão de doença e efeitos de tratamento de doenças ou distúrbios de proliferação celular, tal como câncer, e na triagem de compostos, e.g. novos candidatos de droga, afetando vias enzimáticas, que podem obstruir a formação de timidina fosfatos ou interferir com a síntese de ácido nucleico.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0500432 | 2005-02-25 | ||
| SE0500432-0 | 2005-02-25 | ||
| PCT/SE2006/000246 WO2006091158A1 (en) | 2005-02-25 | 2006-02-24 | A method and kit for determination of thymidine kinase activity and use thereof |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| BRPI0609044A2 true BRPI0609044A2 (pt) | 2011-08-30 |
| BRPI0609044A8 BRPI0609044A8 (pt) | 2017-02-14 |
| BRPI0609044B1 BRPI0609044B1 (pt) | 2020-03-10 |
| BRPI0609044B8 BRPI0609044B8 (pt) | 2021-07-27 |
Family
ID=36927697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0609044A BRPI0609044B8 (pt) | 2005-02-25 | 2006-02-24 | método e kit de ensaio para a determinação de atividade de timidina cinase em uma amostra biológica, uso de método e/ou de kit de ensaio, método para uso em diagnóstico, monitoramento e/ou prognóstico de doenças ou distúrbios de proliferação celular, e, método para uso em triagem de compostos |
Country Status (21)
| Country | Link |
|---|---|
| US (2) | US8765378B2 (pt) |
| EP (1) | EP1856275B1 (pt) |
| JP (1) | JP5227034B2 (pt) |
| KR (1) | KR101275585B1 (pt) |
| CN (1) | CN101128600B (pt) |
| AT (1) | ATE439451T1 (pt) |
| AU (1) | AU2006217129B2 (pt) |
| BR (1) | BRPI0609044B8 (pt) |
| CA (1) | CA2598726C (pt) |
| DE (1) | DE602006008435D1 (pt) |
| DK (1) | DK1856275T3 (pt) |
| EA (1) | EA012438B1 (pt) |
| ES (1) | ES2330881T3 (pt) |
| IL (1) | IL185425A (pt) |
| MX (1) | MX2007010334A (pt) |
| NO (1) | NO341196B1 (pt) |
| NZ (1) | NZ556764A (pt) |
| PL (1) | PL1856275T3 (pt) |
| PT (1) | PT1856275E (pt) |
| WO (1) | WO2006091158A1 (pt) |
| ZA (1) | ZA200707334B (pt) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9429518B2 (en) * | 2010-05-14 | 2016-08-30 | Csens Ab | Kit and method |
| US8728751B2 (en) * | 2011-04-12 | 2014-05-20 | Veterinary Diagnostics Institute, Inc. | System and method for diagnosing lymphoma in cats |
| WO2013048952A1 (en) * | 2011-09-28 | 2013-04-04 | Biomed Valley Discoveries, Inc. | Methods and compositions for detecting infections |
| CN103728456B (zh) * | 2013-09-16 | 2015-09-30 | 长沙赢润生物技术有限公司 | 胸腺嘧啶核苷激酶的elisa诊断试剂盒 |
| CN106556592B (zh) * | 2015-09-24 | 2019-05-21 | 深圳市安群生物工程有限公司 | 定量检测人tk1的化学发光试剂盒及其制备方法 |
| ES2983797T3 (es) * | 2018-08-31 | 2024-10-24 | Hoffmann La Roche | Timidina cinasa (TK-1) en índices pronóstico para LDLBG |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE453514B (sv) | 1982-05-14 | 1988-02-08 | Gronowitz Jan Sim0N | Sett att bestemma deoxitymidinkinas-dtk-isoenzymnivaer i kroppsvetska eller cellprov och anvendningen av forfarandet for isoenzymtypning |
| SE462497B (sv) * | 1988-11-30 | 1990-07-02 | Clas Fredrik Runesson Kaelland | Saett foer att aktivitetsbestaemma polymeraser |
| KR100396548B1 (ko) * | 2001-01-10 | 2003-09-03 | 삼성전자주식회사 | 프린팅 속도를 향상시키는 프린터 제어 장치 및 그 방법 |
| DE60208787T2 (de) * | 2001-06-14 | 2006-11-02 | Cavidi Tech Ab | Verfahren zum messen der polymerisierung von dna und anwendungen des verfahrens |
| WO2004011627A2 (en) * | 2002-07-25 | 2004-02-05 | David-Nicolas Chaperon | A recombinant host cell line screening system for drugs with potential anti-cancer and anti-(retro-) viral activity |
| ATE352039T1 (de) * | 2002-07-27 | 2007-02-15 | Diasorin Ab | Verfahren zur bestimmung der aktivität von thymidinkinase-1 und dessen verwendung |
| DE602004029156D1 (de) * | 2003-05-16 | 2010-10-28 | Arocell Ab | Prognose des verlaufs einer krebserkrankung |
| DE102004032872A1 (de) * | 2004-07-01 | 2006-01-19 | Bayer Cropscience Ag | Verfahren zum Identifizieren von fungizid wirksamen Verbindungen basierend auf Thymidylat-Kinasen |
-
2006
- 2006-02-24 CA CA2598726A patent/CA2598726C/en not_active Expired - Lifetime
- 2006-02-24 MX MX2007010334A patent/MX2007010334A/es active IP Right Grant
- 2006-02-24 PT PT06716936T patent/PT1856275E/pt unknown
- 2006-02-24 BR BRPI0609044A patent/BRPI0609044B8/pt not_active IP Right Cessation
- 2006-02-24 EP EP06716936A patent/EP1856275B1/en not_active Expired - Lifetime
- 2006-02-24 ZA ZA200707334A patent/ZA200707334B/xx unknown
- 2006-02-24 ES ES06716936T patent/ES2330881T3/es not_active Expired - Lifetime
- 2006-02-24 AT AT06716936T patent/ATE439451T1/de active
- 2006-02-24 PL PL06716936T patent/PL1856275T3/pl unknown
- 2006-02-24 AU AU2006217129A patent/AU2006217129B2/en not_active Ceased
- 2006-02-24 EA EA200701572A patent/EA012438B1/ru unknown
- 2006-02-24 US US11/883,187 patent/US8765378B2/en active Active
- 2006-02-24 DE DE602006008435T patent/DE602006008435D1/de not_active Expired - Lifetime
- 2006-02-24 JP JP2007556999A patent/JP5227034B2/ja not_active Expired - Fee Related
- 2006-02-24 KR KR1020077019117A patent/KR101275585B1/ko not_active Expired - Lifetime
- 2006-02-24 NZ NZ556764A patent/NZ556764A/en not_active IP Right Cessation
- 2006-02-24 CN CN200680005892.8A patent/CN101128600B/zh not_active Expired - Fee Related
- 2006-02-24 WO PCT/SE2006/000246 patent/WO2006091158A1/en not_active Ceased
- 2006-02-24 DK DK06716936T patent/DK1856275T3/da active
-
2007
- 2007-08-21 IL IL185425A patent/IL185425A/en active IP Right Grant
- 2007-09-25 NO NO20074882A patent/NO341196B1/no unknown
-
2014
- 2014-05-23 US US14/286,130 patent/US9376707B2/en active Active
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