NO20074882L - En fremgangsmate og kit for bestemmelse av tymidin kinaseaktivitet og deres anvendelse - Google Patents
En fremgangsmate og kit for bestemmelse av tymidin kinaseaktivitet og deres anvendelseInfo
- Publication number
- NO20074882L NO20074882L NO20074882A NO20074882A NO20074882L NO 20074882 L NO20074882 L NO 20074882L NO 20074882 A NO20074882 A NO 20074882A NO 20074882 A NO20074882 A NO 20074882A NO 20074882 L NO20074882 L NO 20074882L
- Authority
- NO
- Norway
- Prior art keywords
- biological sample
- assay kit
- activity
- deoxy nucleoside
- template
- Prior art date
Links
- 102000006601 Thymidine Kinase Human genes 0.000 title abstract 5
- 108020004440 Thymidine kinase Proteins 0.000 title abstract 5
- 230000000694 effects Effects 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 2
- 238000003149 assay kit Methods 0.000 abstract 3
- 239000012472 biological sample Substances 0.000 abstract 3
- 239000002777 nucleoside Substances 0.000 abstract 3
- 150000003833 nucleoside derivatives Chemical class 0.000 abstract 3
- 229910019142 PO4 Inorganic materials 0.000 abstract 2
- 108091000080 Phosphotransferase Proteins 0.000 abstract 2
- 210000001175 cerebrospinal fluid Anatomy 0.000 abstract 2
- -1 new drug candidates Chemical class 0.000 abstract 2
- 235000021317 phosphate Nutrition 0.000 abstract 2
- 102000020233 phosphotransferase Human genes 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 abstract 1
- 206010003445 Ascites Diseases 0.000 abstract 1
- WOVKYSAHUYNSMH-UHFFFAOYSA-N BROMODEOXYURIDINE Natural products C1C(O)C(CO)OC1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-UHFFFAOYSA-N 0.000 abstract 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 abstract 1
- 206010061818 Disease progression Diseases 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Natural products O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 abstract 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 229950004398 broxuridine Drugs 0.000 abstract 1
- 201000011510 cancer Diseases 0.000 abstract 1
- 208000035269 cancer or benign tumor Diseases 0.000 abstract 1
- 229940041514 candida albicans extract Drugs 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 230000005750 disease progression Effects 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 229940000406 drug candidate Drugs 0.000 abstract 1
- 230000009483 enzymatic pathway Effects 0.000 abstract 1
- 239000000284 extract Substances 0.000 abstract 1
- ODKNJVUHOIMIIZ-RRKCRQDMSA-N floxuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(F)=C1 ODKNJVUHOIMIIZ-RRKCRQDMSA-N 0.000 abstract 1
- 229960004716 idoxuridine Drugs 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 239000002547 new drug Substances 0.000 abstract 1
- 238000001668 nucleic acid synthesis Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 210000002381 plasma Anatomy 0.000 abstract 1
- 210000004910 pleural fluid Anatomy 0.000 abstract 1
- 230000000379 polymerizing effect Effects 0.000 abstract 1
- 238000004393 prognosis Methods 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 229940104230 thymidine Drugs 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 abstract 1
- 239000012138 yeast extract Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Fremgangsmåte og analysesett for bestemmelse av tymidinkinase (TK)-aktivitet i en biologisk prøve, slik som blod, serum, plasma, Cerebral Spinal Væske (CSF), pleuralvæske, ascites, vev, celler og ekstrakter av disse beskrives. Fremgangsmåten omfatter å bringe, i en buffer, en basisk reaksjonsblanding omfattende fastfasekoblet primer og/eller templat, et modifisert deoksynukleosid slik som BromdeoksyUridin, JoddeoksyUridin, FluordeoksyUridin eller VinyldeoksyTymidin som et kinaseenzymsubstrat, en fosfatdonor, et nukleotidpolymeriserende enzym og en kinaseenzymkilde fri for TK-aktivitet slik som et gjærekstrakt, i kontakt med den biologiske prøven. Etter inkubering bestemmes mengden modifisert deoksynukleosid som er blitt innkorporert i den fastfasekoblede primer og/eller templat og TK-aktiviteten tilstede i den biologiske prøven er direkte proporsjonal med mengden innkorporert modifisert deoksynukleosid. Fremgangsmåten og analysesettet er nyttig ved diagnostisering, prognosekontroll av sykdomsutviklingen og behandling av effekter ved celleprolifereringsforstyrrelser og sykdommer slik som cancer, og ved screening av forbindelser for eksempel nye medikamentkandidater som påvirker enzymatiske reaksjonsveier som kan hindre dannelse av tymidinfosfater eller interferere med nukleinsyresyntesen.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0500432 | 2005-02-25 | ||
| PCT/SE2006/000246 WO2006091158A1 (en) | 2005-02-25 | 2006-02-24 | A method and kit for determination of thymidine kinase activity and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NO20074882L true NO20074882L (no) | 2007-11-22 |
| NO341196B1 NO341196B1 (no) | 2017-09-11 |
Family
ID=36927697
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO20074882A NO341196B1 (no) | 2005-02-25 | 2007-09-25 | Fremgangsmåte og analysesett for bestemmelse av tymidinkinaseaktivitet og anvendelse av disse |
Country Status (21)
| Country | Link |
|---|---|
| US (2) | US8765378B2 (no) |
| EP (1) | EP1856275B1 (no) |
| JP (1) | JP5227034B2 (no) |
| KR (1) | KR101275585B1 (no) |
| CN (1) | CN101128600B (no) |
| AT (1) | ATE439451T1 (no) |
| AU (1) | AU2006217129B2 (no) |
| BR (1) | BRPI0609044B8 (no) |
| CA (1) | CA2598726C (no) |
| DE (1) | DE602006008435D1 (no) |
| DK (1) | DK1856275T3 (no) |
| EA (1) | EA012438B1 (no) |
| ES (1) | ES2330881T3 (no) |
| IL (1) | IL185425A (no) |
| MX (1) | MX2007010334A (no) |
| NO (1) | NO341196B1 (no) |
| NZ (1) | NZ556764A (no) |
| PL (1) | PL1856275T3 (no) |
| PT (1) | PT1856275E (no) |
| WO (1) | WO2006091158A1 (no) |
| ZA (1) | ZA200707334B (no) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2569441T4 (en) * | 2010-05-14 | 2017-12-18 | Csens Ab | KIT AND METHOD OF MEASURING THE ACTIVITY OF DEOXYNUCLEOSIDE KINASE USING DNA DEPENDENT DNA POLYMERASE AND NATURAL DEOXYNUCLEOSIDES |
| US8728751B2 (en) * | 2011-04-12 | 2014-05-20 | Veterinary Diagnostics Institute, Inc. | System and method for diagnosing lymphoma in cats |
| US20140228248A1 (en) * | 2011-09-28 | 2014-08-14 | Biomed Valley Discoveries, Inc. | Methods and compositions for detecting infections |
| CN103728456B (zh) * | 2013-09-16 | 2015-09-30 | 长沙赢润生物技术有限公司 | 胸腺嘧啶核苷激酶的elisa诊断试剂盒 |
| CN106556592B (zh) * | 2015-09-24 | 2019-05-21 | 深圳市安群生物工程有限公司 | 定量检测人tk1的化学发光试剂盒及其制备方法 |
| JP7577647B2 (ja) * | 2018-08-31 | 2024-11-05 | エフ． ホフマン－ラ ロシュ アーゲー | Ｄｌｂｃｌについての予後指標におけるチミジンキナーゼ（ｔｋ－１） |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE453514B (sv) * | 1982-05-14 | 1988-02-08 | Gronowitz Jan Sim0N | Sett att bestemma deoxitymidinkinas-dtk-isoenzymnivaer i kroppsvetska eller cellprov och anvendningen av forfarandet for isoenzymtypning |
| SE462497B (sv) | 1988-11-30 | 1990-07-02 | Clas Fredrik Runesson Kaelland | Saett foer att aktivitetsbestaemma polymeraser |
| KR100396548B1 (ko) * | 2001-01-10 | 2003-09-03 | 삼성전자주식회사 | 프린팅 속도를 향상시키는 프린터 제어 장치 및 그 방법 |
| EP1395675B1 (en) * | 2001-06-14 | 2006-01-18 | Cavidi Tech AB | A method for measuring dna polymerization and applications of the method |
| WO2004011627A2 (en) * | 2002-07-25 | 2004-02-05 | David-Nicolas Chaperon | A recombinant host cell line screening system for drugs with potential anti-cancer and anti-(retro-) viral activity |
| DE60217665T2 (de) * | 2002-07-27 | 2007-10-11 | Diasorin Ab | Verfahren zur Bestimmung der Aktivität von Thymidinkinase-1 und dessen Verwendung |
| ES2353014T3 (es) * | 2003-05-16 | 2011-02-24 | Arocell Ab | Predicción de la evolución del cáncer. |
| DE102004032872A1 (de) * | 2004-07-01 | 2006-01-19 | Bayer Cropscience Ag | Verfahren zum Identifizieren von fungizid wirksamen Verbindungen basierend auf Thymidylat-Kinasen |
-
2006
- 2006-02-24 KR KR1020077019117A patent/KR101275585B1/ko not_active Expired - Fee Related
- 2006-02-24 CA CA2598726A patent/CA2598726C/en not_active Expired - Lifetime
- 2006-02-24 AU AU2006217129A patent/AU2006217129B2/en not_active Ceased
- 2006-02-24 EP EP06716936A patent/EP1856275B1/en not_active Expired - Lifetime
- 2006-02-24 NZ NZ556764A patent/NZ556764A/en not_active IP Right Cessation
- 2006-02-24 DK DK06716936T patent/DK1856275T3/da active
- 2006-02-24 US US11/883,187 patent/US8765378B2/en active Active
- 2006-02-24 CN CN200680005892.8A patent/CN101128600B/zh not_active Expired - Fee Related
- 2006-02-24 EA EA200701572A patent/EA012438B1/ru unknown
- 2006-02-24 JP JP2007556999A patent/JP5227034B2/ja not_active Expired - Fee Related
- 2006-02-24 ES ES06716936T patent/ES2330881T3/es not_active Expired - Lifetime
- 2006-02-24 DE DE602006008435T patent/DE602006008435D1/de not_active Expired - Lifetime
- 2006-02-24 BR BRPI0609044A patent/BRPI0609044B8/pt not_active IP Right Cessation
- 2006-02-24 PT PT06716936T patent/PT1856275E/pt unknown
- 2006-02-24 MX MX2007010334A patent/MX2007010334A/es active IP Right Grant
- 2006-02-24 WO PCT/SE2006/000246 patent/WO2006091158A1/en not_active Ceased
- 2006-02-24 ZA ZA200707334A patent/ZA200707334B/xx unknown
- 2006-02-24 AT AT06716936T patent/ATE439451T1/de active
- 2006-02-24 PL PL06716936T patent/PL1856275T3/pl unknown
-
2007
- 2007-08-21 IL IL185425A patent/IL185425A/en active IP Right Grant
- 2007-09-25 NO NO20074882A patent/NO341196B1/no unknown
-
2014
- 2014-05-23 US US14/286,130 patent/US9376707B2/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| CHAD | Change of the owner's name or address (par. 44 patent law, par. patentforskriften) |
Owner name: BIOVICA INTERNATIONAL AB, SE |