BRPI0713252B8 - processo para a recuperação de fator de crescimento endotelial vascular (vegf) recombinante reenovelado - Google Patents

processo para a recuperação de fator de crescimento endotelial vascular (vegf) recombinante reenovelado

Info

Publication number
BRPI0713252B8
BRPI0713252B8 BRPI0713252A BRPI0713252A BRPI0713252B8 BR PI0713252 B8 BRPI0713252 B8 BR PI0713252B8 BR PI0713252 A BRPI0713252 A BR PI0713252A BR PI0713252 A BRPI0713252 A BR PI0713252A BR PI0713252 B8 BRPI0713252 B8 BR PI0713252B8
Authority
BR
Brazil
Prior art keywords
vegf
growth factor
vascular endothelial
endothelial growth
recovered recombinant
Prior art date
Application number
BRPI0713252A
Other languages
English (en)
Inventor
Sanchez Ailen
H Schmelzer Charles
Pizarro Shelly
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of BRPI0713252A2 publication Critical patent/BRPI0713252A2/pt
Publication of BRPI0713252B1 publication Critical patent/BRPI0713252B1/pt
Publication of BRPI0713252B8 publication Critical patent/BRPI0713252B8/pt
Publication of BRPI0713252C1 publication Critical patent/BRPI0713252C1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

processos para a recuperação de uma proteína recombinante reenovelada e método para purificar uma proteína recombinante. são fornecidos processos para a recuperação e purificação de proteínas recombinantes reenoveladas produzidas em células hospedeiras heteróloga, que inclui a etapa de reenovelamento da proteína em um tampão de ph elevado.
BRPI0713252A 2006-07-14 2007-07-13 processo para a recuperação de fator de crescimento endotelial vascular (vegf) recombinante reenovelado BRPI0713252C1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US83083106P 2006-07-14 2006-07-14
US60/830,831 2006-07-14
PCT/US2007/073496 WO2008008975A2 (en) 2006-07-14 2007-07-13 Refolding of recombinant proteins

Publications (4)

Publication Number Publication Date
BRPI0713252A2 BRPI0713252A2 (pt) 2012-04-10
BRPI0713252B1 BRPI0713252B1 (pt) 2018-12-18
BRPI0713252B8 true BRPI0713252B8 (pt) 2019-04-09
BRPI0713252C1 BRPI0713252C1 (pt) 2021-05-25

Family

ID=38722613

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0713252A BRPI0713252C1 (pt) 2006-07-14 2007-07-13 processo para a recuperação de fator de crescimento endotelial vascular (vegf) recombinante reenovelado

Country Status (18)

Country Link
US (4) US20080125580A1 (pt)
EP (1) EP2041154B1 (pt)
JP (2) JP5566104B2 (pt)
KR (1) KR101520105B1 (pt)
CN (1) CN101506222B (pt)
AR (1) AR062069A1 (pt)
AU (1) AU2007272412B2 (pt)
BR (1) BRPI0713252C1 (pt)
CA (1) CA2656835C (pt)
IL (1) IL196004A (pt)
MX (1) MX2009000278A (pt)
MY (1) MY169472A (pt)
NZ (1) NZ573639A (pt)
RU (1) RU2439076C2 (pt)
SG (1) SG162834A1 (pt)
TW (1) TWI476207B (pt)
WO (1) WO2008008975A2 (pt)
ZA (1) ZA200900229B (pt)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8329871B2 (en) 2008-06-24 2012-12-11 Octapharma Ag Process of purifying coagulation factor VIII
WO2010020690A1 (en) 2008-08-21 2010-02-25 Octapharma Ag Recombinantly produced human factor viii and ix
KR101063347B1 (ko) 2009-04-06 2011-09-07 인하대학교 산학협력단 이온성 액체를 이용한 이황화 결합을 포함하는 단백질의 재접힘 방법
US8067201B2 (en) * 2009-04-17 2011-11-29 Bristol-Myers Squibb Company Methods for protein refolding
SG176963A1 (en) 2009-06-22 2012-02-28 Amgen Inc Refolding proteins using a chemically controlled redox state
LT3660032T (lt) 2009-06-25 2026-02-25 Amgen Inc. Ne žinduolių sistemoje ekspresuotų baltymų surišamojo gryninimo būdai
CA2815342A1 (en) * 2010-10-20 2012-04-26 Medimmune, Llc Methods for processing inclusion bodies
US20140087419A1 (en) * 2011-04-28 2014-03-27 Riken Method for making biological material transparent and use thereof
EP2711682B1 (en) 2011-05-20 2019-07-10 Riken Clarifying reagent for biological materials and use thereof
CN102443055B (zh) * 2011-10-26 2017-07-28 浙江海正药业股份有限公司 一种重组人肿瘤坏死因子相关凋亡诱导配体的纯化工艺
RU2492177C2 (ru) * 2011-12-15 2013-09-10 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП ГосНИИгенетика) СПОСОБ ВЫДЕЛЕНИЯ И ОЧИСТКИ РЕКОМБИНАНТНОГО ГОРМОНА РОСТА ЧЕЛОВЕКА, СЕКРЕТИРУЕМОГО ДРОЖЖАМИ Saccharomyces cerevisiae
SG11201406112TA (en) * 2012-03-27 2014-10-30 Genentech Inc Improved harvest operations for recombinant proteins
CN103588871B (zh) * 2012-08-16 2016-08-03 深圳新鹏生物工程有限公司 一种rhTRAIL菌体的分离纯化方法
CN105007937B (zh) * 2012-12-20 2019-11-19 米迪缪尼有限公司 生产免疫偶联物的方法
US10065987B2 (en) * 2013-02-12 2018-09-04 Bristol-Myers Squibb Company High pH protein refolding methods
CN105611909B (zh) 2013-03-12 2020-09-15 普莱玛疗法公司 包含螯合剂和碱的牙用组合物
US9982012B2 (en) 2013-03-29 2018-05-29 Dr. Reddy's Laboratories Limited Refolding of granulocyte colony stimulating factor
JP6433901B2 (ja) 2013-08-14 2018-12-05 国立研究開発法人理化学研究所 光透過性に優れた生物材料を調製するための組成物およびその利用
CN103694339B (zh) * 2013-12-04 2017-10-13 珠海联邦制药股份有限公司 一种甘精胰岛素前体的复性方法
KR101651330B1 (ko) * 2014-07-28 2016-08-25 가톨릭대학교 산학협력단 세포투과성이 우수한 ｔａｔ－ａ２０ 융합단백질의 제조방법 및 이의 용도
EP3221449B1 (en) * 2014-11-18 2023-08-30 Merck Sharp & Dohme LLC Process for refolding recombinant chymotrypsin
US10947521B2 (en) 2014-11-18 2021-03-16 Merck Sharp & Dohme Corp. Process for producing recombinant trypsin
ES3046837T3 (en) 2014-12-22 2025-12-02 UCB Biopharma SRL Method of protein manufacture
CN107108712A (zh) * 2014-12-23 2017-08-29 阿尔莫生物科技股份有限公司 提高重组蛋白产量的方法
HUE052993T2 (hu) * 2015-08-17 2021-06-28 Lupin Ltd Fejlesztett újrahajtogatási eljárás antitest fragmensekhez
HUE049756T2 (hu) * 2015-11-09 2020-10-28 Biological E Ltd Ipari méretekben is alkalmazható eljárás biológiailag aktív, rekombináns hordozófehérjék kinyerésére
CN106008702B (zh) * 2016-07-22 2019-11-26 江苏江山聚源生物技术有限公司 一种规模化分离纯化重组人源胶原蛋白的方法
US11479591B2 (en) 2017-05-05 2022-10-25 Trefoil Therapeutics, Inc. Recombinant modified fibroblast growth factors and therapeutic uses thereof
EP3624847A1 (en) 2017-05-19 2020-03-25 Council of Scientific and Industrial Research A method for producing refolded recombinant humanized ranibizumab
EP3959222A4 (en) * 2019-04-24 2023-06-14 Tanvex Biopharma Usa, Inc. Process for preparing granulocyte-colony stimulating factor
CN116710568A (zh) * 2020-06-26 2023-09-05 特里福伊尔治疗公司 重组修饰成纤维细胞生长因子及其治疗用途
CN111848774B (zh) * 2020-08-05 2022-05-10 武汉海特生物制药股份有限公司 一种美曲普汀的制备方法
KR102534015B1 (ko) * 2021-01-27 2023-05-19 (재)씨젠의료재단 그람 음성균 유래 원형 단백질의 분리 방법
WO2023017540A1 (en) * 2021-08-11 2023-02-16 Tata Institute For Genetics And Society Methods for refolding a solubilized recombinant protein and implementation thereof

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4169950A (en) 1977-12-08 1979-10-02 Research Organics Amino-hydroxy-alkyl sulfonic acid zwitterions
US4565785A (en) * 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
EP0104771B1 (en) 1982-09-02 1990-05-23 Nellcor Incorporated Pulse oximeter monitor
US4673641A (en) * 1982-12-16 1987-06-16 Molecular Genetics Research And Development Limited Partnership Co-aggregate purification of proteins
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4490473A (en) * 1983-03-28 1984-12-25 Panab Labeled antibodies and methods
US4710473A (en) 1983-08-10 1987-12-01 Amgen, Inc. DNA plasmids
US4795706A (en) * 1985-01-31 1989-01-03 Eli Lilly And Company Novel expression control sequences
ES8800957A1 (es) * 1985-02-22 1987-12-01 Monsanto Co Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina
US4652630A (en) 1985-02-22 1987-03-24 Monsanto Company Method of somatotropin naturation
DE3537708A1 (de) 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US5453363A (en) * 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
DE3835350A1 (de) 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
US5064943A (en) * 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
US5332671A (en) 1989-05-12 1994-07-26 Genetech, Inc. Production of vascular endothelial cell growth factor and DNA encoding same
US5194596A (en) 1989-07-27 1993-03-16 California Biotechnology Inc. Production of vascular endothelial cell growth factor
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
DE4037196A1 (de) 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
US5322699A (en) * 1991-02-04 1994-06-21 The Rockefeller University Leukocyte-derived CR3 modulator, integrin modulating factor-1 (IMF-1)
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
US5331095A (en) 1993-04-12 1994-07-19 Scios Nova Inc. Process for purification of basic fibroblast growth factor
JP4098372B2 (ja) 1993-07-28 2008-06-11 三菱化学株式会社 ヘパリン結合性増殖因子の生産方法
US5663304A (en) * 1993-08-20 1997-09-02 Genentech, Inc. Refolding of misfolded insulin-like growth factor-I
US5464815A (en) 1993-09-08 1995-11-07 Genentech, Inc. Inhibition of heparin-binding
NZ270271A (en) * 1994-01-05 1996-07-26 Lilly Co Eli Minimizing protein c degradation
DK0751992T3 (da) 1994-03-08 2006-03-06 Human Genome Sciences Inc Karendotelvækstfaktor 2
FR2729972B1 (fr) 1995-01-31 1997-04-18 Sanofi Sa Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine
SE9504019D0 (sv) 1995-11-13 1995-11-13 Pharmacia Ab Method for production of proteins
US6750044B1 (en) 1996-10-17 2004-06-15 Genentech, Inc. Variants of vascular endothelial cell growth factor having antagonistic properties, nucleic acids encoding the same and host cells comprising those nucleic acids
US6632425B1 (en) 1997-03-20 2003-10-14 Human Genome Sciences, Inc. Chemokine compositions
DE19735711C2 (de) * 1997-08-18 2001-04-26 Aventis Pharma Gmbh Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
ATE408631T1 (de) 1998-03-31 2008-10-15 Tonghua Gantech Biotechnology Chimäres protein welches eine intramolekulare chaperon-ähnliche sequenz enthält und dessen anwendung zur insulinproduktion
ES2228052T3 (es) 1998-06-01 2005-04-01 Genentech, Inc. Separacion demonomeros de anticuerpos de sus multimeros utilizando cromatografia de intercambio de iones.
PT1124941E (pt) 1998-10-28 2004-02-27 Genentech Inc Processo para recuperar polipeptidos heterologos a partir de celulas bacterianas
US6783953B1 (en) 1998-12-22 2004-08-31 Janssen Pharmaceutica N.V. Vascular endothelial growth factor-X
CA2374050A1 (en) 1999-05-20 2000-11-30 Scios Inc. Vascular endothelial growth factor dimers
US6206768B1 (en) * 1999-07-29 2001-03-27 Chartered Semiconductor Manufacturing, Ltd. Adjustable and extended guide rings
DK1255769T3 (da) * 2000-01-25 2007-09-03 Oklahoma Med Res Found Universel procedure til genfoldning af rekombinante proteiner
AUPQ568100A0 (en) 2000-02-16 2000-03-09 Amrad Operations Pty. Limited A method for producing recombinant molecules
WO2001075095A1 (fr) * 2000-03-30 2001-10-11 Takeda Chemical Industries, Ltd. Procede de production de proteine de recombinaison
NZ522847A (en) 2000-05-16 2004-11-26 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
US8632778B2 (en) * 2000-08-11 2014-01-21 Chugai Seiyaku Kabushiki Kaisha Stabilized anti-interleukin-6 antibody-containing preparations
DE10054059A1 (de) 2000-10-31 2002-05-08 Max Delbrueck Centrum Verfahren zur Ermittlung von Faltungsbedingungen für rekombinante Proteine
WO2002057296A1 (en) 2001-01-16 2002-07-25 Københavns Universitet A method for refolding of proteins
US7611711B2 (en) 2001-01-17 2009-11-03 Vegenics Limited VEGFR-3 inhibitor materials and methods
EE200300408A (et) 2001-02-23 2003-12-15 Immunex Corporation Aktiivsete valkude saagise suurendamine
SI1501369T1 (sl) * 2002-04-26 2015-10-30 Genentech, Inc. Neafinitetno čiščenje proteinov
CN1207307C (zh) * 2002-11-21 2005-06-22 李越希 重组人巨细胞病毒融合蛋白及其制备方法、应用
NZ540895A (en) 2003-01-09 2007-03-30 Genentech Inc Purification of polypeptides with ethacridine lactate
EP1449848A1 (en) * 2003-02-20 2004-08-25 GBF German Research Centre for Biotechnology Method for the production of cystine-knot proteins
CN1473932A (zh) * 2003-06-30 2004-02-11 中山大学 一种重组石斑鱼腺苷酸环化酶激活多肽基因及其表达系统、表达产物、生产方法和应用
EP2270163A1 (en) * 2003-12-10 2011-01-05 Eli Lilly and Company Muteins of fibroblast growth factor 21
JP2007535911A (ja) 2003-12-30 2007-12-13 バハラ バイオテック インターナショナル リミテッド 組換えタンパク質の製造及び精製方法
US8084032B2 (en) * 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
DE102004015965A1 (de) * 2004-04-01 2005-10-20 Aventis Pharma Gmbh Verfahren zur Gewinnung von Insulinen durch verbesserte Luftbegasung der Faltung
AU2005257064B2 (en) * 2004-06-28 2012-05-10 Yeda Research And Development Co. Ltd. Chimeric proteins and uses thereof
WO2006053568A1 (en) * 2004-11-22 2006-05-26 Borean Pharma Aps Tnf antagonists
DE602006003108D1 (de) * 2005-03-03 2008-11-27 Ajinomoto Kk Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
MY143274A (en) 2005-12-22 2011-04-15 Genentech Inc Recombinant production of heparin binding proteins

Also Published As

Publication number Publication date
US9994612B2 (en) 2018-06-12
US20130123474A1 (en) 2013-05-16
EP2041154A2 (en) 2009-04-01
IL196004A (en) 2014-06-30
WO2008008975A2 (en) 2008-01-17
CA2656835C (en) 2017-12-19
RU2009105080A (ru) 2010-08-27
BRPI0713252B1 (pt) 2018-12-18
KR20090039772A (ko) 2009-04-22
AU2007272412B2 (en) 2013-11-07
MY169472A (en) 2019-04-12
BRPI0713252C1 (pt) 2021-05-25
JP6120356B2 (ja) 2017-04-26
TWI476207B (zh) 2015-03-11
EP2041154B1 (en) 2018-12-12
MX2009000278A (es) 2009-01-26
US20160137690A1 (en) 2016-05-19
AU2007272412A1 (en) 2008-01-17
CN101506222B (zh) 2013-10-02
WO2008008975A8 (en) 2008-10-16
BRPI0713252A2 (pt) 2012-04-10
US20110294990A1 (en) 2011-12-01
TW200813093A (en) 2008-03-16
RU2439076C2 (ru) 2012-01-10
IL196004A0 (en) 2011-08-01
US20080125580A1 (en) 2008-05-29
JP5566104B2 (ja) 2014-08-06
WO2008008975A3 (en) 2008-04-10
JP2013121362A (ja) 2013-06-20
US9200030B2 (en) 2015-12-01
SG162834A1 (en) 2010-07-29
CN101506222A (zh) 2009-08-12
AR062069A1 (es) 2008-10-15
KR101520105B1 (ko) 2015-05-21
ZA200900229B (en) 2010-04-28
JP2009543882A (ja) 2009-12-10
CA2656835A1 (en) 2008-01-17
NZ573639A (en) 2012-03-30

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