BRPI9600729A - vetor de expressão, microorganismo transgênico, processo de clonagem de um ácido nucléico e processo para preparação de uma proteína e cepa de levedura transformada - Google Patents

vetor de expressão, microorganismo transgênico, processo de clonagem de um ácido nucléico e processo para preparação de uma proteína e cepa de levedura transformada

Info

Publication number
BRPI9600729A
BRPI9600729A BRPI9600729A BRPI9600729A BRPI9600729A BR PI9600729 A BRPI9600729 A BR PI9600729A BR PI9600729 A BRPI9600729 A BR PI9600729A BR PI9600729 A BRPI9600729 A BR PI9600729A BR PI9600729 A BRPI9600729 A BR PI9600729A
Authority
BR
Brazil
Prior art keywords
protein
cloning
preparing
nucleic acid
yeast strain
Prior art date
Application number
BRPI9600729A
Other languages
English (en)
Inventor
Xavier Chenivesse
Catherine Duport
Eric Lecain
Denis Pompon
Original Assignee
Roussel Uclaf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9501723A external-priority patent/FR2730494B1/fr
Priority claimed from FR9506517A external-priority patent/FR2734839B1/fr
Application filed by Roussel Uclaf filed Critical Roussel Uclaf
Publication of BRPI9600729A publication Critical patent/BRPI9600729A/pt
Publication of BRPI9600729B1 publication Critical patent/BRPI9600729B1/pt
Publication of BRPI9600729B8 publication Critical patent/BRPI9600729B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/02—Dehydrogenating; Dehydroxylating

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

sequências de adnc, proteína de a. thaliana, anticorpo, vetor, microorganismo transgênico, processo de clonagem de um ácido nucléico, processo para preparação de uma proteína, processo de redução in vitro ou in vivo de um esterol, processo de produção de pregnenolona, cepa de levedura transformada e método de deteção da deficiência em delta-5,7-esterol, delta-7-redutase. sequência de adnc codificando para uma proteína de a. thaliana que tem uma atividade delta-5,7-esterol, delta-7-redutase (seguência seq id nº 1). processo de clonagem. processo de redução de um esterol insaturado em posição c-7. processo de produção de pregnenolona.
BRPI9600729 1995-02-15 1996-02-14 vetor de expressão, microorganismo transgênico, processo de clonagem de um ácido nucléico e processo para preparação de uma proteína e cepa de levedura transformada BRPI9600729B8 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FR9501723A FR2730494B1 (fr) 1995-02-15 1995-02-15 Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta7-red, procede de production, souches de levures transformees, applications
FR9501723 1995-02-15
FR9506517 1995-06-01
FR9506517A FR2734839B1 (fr) 1995-06-01 1995-06-01 Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta-7red, procede de production, souches de levures transformees, applications.

Publications (3)

Publication Number Publication Date
BRPI9600729A true BRPI9600729A (pt) 1997-12-23
BRPI9600729B1 BRPI9600729B1 (pt) 2017-06-20
BRPI9600729B8 BRPI9600729B8 (pt) 2021-07-06

Family

ID=26231759

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9600729 BRPI9600729B8 (pt) 1995-02-15 1996-02-14 vetor de expressão, microorganismo transgênico, processo de clonagem de um ácido nucléico e processo para preparação de uma proteína e cepa de levedura transformada

Country Status (24)

Country Link
US (3) US5759801A (pt)
EP (1) EP0727489B1 (pt)
JP (1) JP4082469B2 (pt)
KR (1) KR100422293B1 (pt)
CN (2) CN1216995C (pt)
AR (2) AR003407A1 (pt)
AT (1) ATE457354T1 (pt)
BR (1) BRPI9600729B8 (pt)
CA (1) CA2169524C (pt)
CZ (1) CZ294860B6 (pt)
DE (1) DE69638123D1 (pt)
DK (1) DK0727489T3 (pt)
ES (1) ES2339833T3 (pt)
FI (1) FI120877B (pt)
HR (1) HRP960078B1 (pt)
HU (1) HU220587B1 (pt)
IL (4) IL116872A (pt)
PL (1) PL185569B1 (pt)
PT (1) PT727489E (pt)
RU (1) RU2242517C2 (pt)
SI (1) SI0727489T1 (pt)
SK (1) SK283656B6 (pt)
TR (1) TR199600116A2 (pt)
UA (1) UA72172C2 (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3493997A (en) * 1996-06-21 1998-01-07 General Hospital Corporation, The Plant sterol reductases and uses thereof
DE19739940A1 (de) * 1997-09-11 1999-03-18 Hartmut Prof Dr Med Glossmann Protein mit Aminosäuresequenz der DELTA7-Sterolreduktase, isoliertes cDNA-Molekül, das für das Protein kodiert, rekombinanter DNA-Vektor und biologisch aktive Zusammensetzungen, die das Protein oder das cDNA-Molekül enthalten
FR2774393B1 (fr) * 1998-02-05 2000-03-24 Hoechst Marion Roussel Inc Souches de levure ayant le gene atf2 interrompu et leurs applications
US6465717B1 (en) * 1998-11-20 2002-10-15 E. I. Du Pont De Nemours And Company Sterol metabolism enzymes
FR2812884B1 (fr) * 2000-08-08 2002-11-08 Aventis Pharma Sa Levures modifiees et utilisations, notamment pour la production de derives steroidiens
FR2820145B1 (fr) 2001-01-31 2004-01-23 Aventis Pharma Sa Souche de levure produisant des steroides de facon autonome
US7193169B2 (en) * 2003-10-29 2007-03-20 Alcon, Inc. Ergonomic footswitch
FR2869914B1 (fr) 2004-05-06 2012-11-09 Aventis Pharma Sa Souches de levure produisant du cholesterol et leurs applications
CA2600882C (en) * 2005-03-16 2013-02-26 Metabolix, Inc. Chemically inducible expression of biosynthetic pathways
KR100672006B1 (ko) * 2005-08-03 2007-01-19 길병옥 폐자재를 이용한 형강의 제조방법 및 그 제조방법에 의해생산된 형강
BRPI0821401A2 (pt) 2007-12-21 2014-10-14 Novartis Ag Compostos orgânicos
DE102009022772A1 (de) 2009-05-21 2010-11-25 Organobalance Gmbh Mikroorganismus zur Expression eines humanen Membranproteins
FR2961218A1 (fr) * 2010-06-10 2011-12-16 Sanofi Aventis Methode de detection de composes modulateurs du metabolisme du cholesterol
CN102286053B (zh) * 2011-06-20 2012-09-05 河北联合大学 孕甾烯醇酮吡咯化合物及其制备方法
US10308972B2 (en) 2013-06-17 2019-06-04 Sanofi Chimie Whole-cell system for cytochrome P450 monooxygenases biocatalysis
CN110903993A (zh) * 2019-12-20 2020-03-24 河北兰升生物科技有限公司 一种产生菜籽甾醇的酿酒酵母工程菌及其构建方法和应用
CN112813129B (zh) * 2021-02-05 2023-09-08 江南大学 利用区室化提高酵母菌中7-脱氢胆固醇产量的方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2554986A (en) * 1950-01-05 1951-05-29 Upjohn Co Synthesis of pregnenolone
JPS6447380A (en) * 1987-08-19 1989-02-21 Agency Ind Science Techn Steroid-oxidizing yeast strain
CN1042567A (zh) * 1988-09-23 1990-05-30 吉斯特-布罗卡迪斯公司 类固醇多步氧化方法和所用的遗传工程细胞
JP2645130B2 (ja) * 1989-03-31 1997-08-25 日清製粉株式会社 ステロイド誘導体
US5212167A (en) * 1991-09-12 1993-05-18 Trustees Of Boston University Modulation of receptor-mediated ion transport
US5547868A (en) * 1993-06-09 1996-08-20 Regents Of The University Of California Cholesterol disposal fusion enzymes

Also Published As

Publication number Publication date
PL312828A1 (en) 1996-08-19
DE69638123D1 (de) 2010-03-25
CZ294860B6 (cs) 2005-04-13
IL116872A0 (en) 1996-07-23
FI960663A0 (fi) 1996-02-14
EP0727489A1 (fr) 1996-08-21
US5989881A (en) 1999-11-23
CN1664107A (zh) 2005-09-07
AR003407A1 (es) 1998-08-05
SI0727489T1 (sl) 2010-05-31
US5759801A (en) 1998-06-02
IL162476A (en) 2005-12-18
AR053931A2 (es) 2007-05-23
HUP9600325A1 (en) 1996-11-28
HRP960078A2 (pt) 1998-02-28
RU2242517C2 (ru) 2004-12-20
DK0727489T3 (da) 2010-05-31
FI960663L (fi) 1996-08-16
PL185569B1 (pl) 2003-06-30
CN1145953A (zh) 1997-03-26
IL162476A0 (en) 2005-11-20
BRPI9600729B1 (pt) 2017-06-20
CN1216995C (zh) 2005-08-31
HRP960078B1 (en) 2011-11-30
TR199600116A2 (tr) 1996-08-21
UA72172C2 (uk) 2005-02-15
CA2169524C (fr) 2012-05-01
CA2169524A1 (fr) 1996-08-16
IL162477A0 (en) 2005-11-20
HUP9600325A3 (en) 1999-07-28
CZ43296A3 (en) 1996-12-11
IL116872A (en) 2004-12-15
FI120877B (fi) 2010-04-15
HU220587B1 (hu) 2002-03-28
SK283656B6 (sk) 2003-11-04
ES2339833T3 (es) 2010-05-25
SK18896A3 (en) 1996-10-02
BRPI9600729B8 (pt) 2021-07-06
EP0727489B1 (fr) 2010-02-10
KR100422293B1 (ko) 2004-06-14
PT727489E (pt) 2010-04-27
KR960031607A (ko) 1996-09-17
JP4082469B2 (ja) 2008-04-30
JPH08289793A (ja) 1996-11-05
ATE457354T1 (de) 2010-02-15
US5965417A (en) 1999-10-12

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Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B25A Requested transfer of rights approved

Owner name: HOECHST MARION ROUSSEL (FR)

Free format text: TRANSFERIDO POR FUSAO DE: ROUSSEL-UCLAF

B25A Requested transfer of rights approved

Owner name: AVENTIS PHARMA S.A. (FR)

Free format text: TRANSFERIDO DE: HOECHST MARION ROUSSEL

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: DE ACORDO COM O ART. 37, INDEFIRO O PRESENTE PEDIDO, UMA VEZ QUE A MATERIA PLEITEADA NAS REIVINDICACOES 1 A 3 NAO ATENDE AO REQUISITO DE ATIVIDADE INVENTIVA (ART. 8O COMBINADO COM ART. 13 DA LPI).

B12B Appeal against refusal [chapter 12.2 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]
B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time
B24D Patent annual fee: restoration after fee payment
B15K Others concerning applications: alteration of classification

Ipc: C12N 15/81 (2006.01), C12N 15/74 (2006.0

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 14/02/96, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO