RO117190B1 - Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă - Google Patents

Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă

Info

Publication number
RO117190B1
RO117190B1 RO97-00422A RO9700422A RO117190B1 RO 117190 B1 RO117190 B1 RO 117190B1 RO 9700422 A RO9700422 A RO 9700422A RO 117190 B1 RO117190 B1 RO 117190B1
Authority
RO
Romania
Prior art keywords
plant
expression
identifying
heterologous gene
nucleic acid
Prior art date
Application number
RO97-00422A
Other languages
English (en)
Inventor
Gabriele Dietrich
Jane Smith
Jianying Peng
Original Assignee
American Cyanamid Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23171273&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=RO117190(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by American Cyanamid Company filed Critical American Cyanamid Company
Publication of RO117190B1 publication Critical patent/RO117190B1/ro

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Invenţia se referă la un construct de acid nucleic ce cuprinde un promotor AHAS pentru expresia genelor, în plante, legat operaţional la o secvenţă care codifică o genă heteroloagă, promotor care poate fi selectat dintre promotorii genelor als 1 şi als 2 de la porumb sau promotorul AHAS ce cuprinde secvenţa SEQ ID No 1, SEQ ID No 2 sau SEQ ID No 3, precum şi la o metodă de identificare a expresiei genice, într-o plantă şi/sau în diferite ţesuturi ale acesteia, şi care constă în transformarea recombinantă a unei porţiuni din plantă, expresia genei heteroloage în porţiunea din plantă, astfel transformată, şi detectarea expresiei genice.
RO97-00422A 1994-09-08 1995-09-05 Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă RO117190B1 (ro)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/303,266 US5750866A (en) 1994-09-08 1994-09-08 AHAS promoter useful for expression of introduced genes in plants
PCT/US1995/011199 WO1996007746A1 (en) 1994-09-08 1995-09-05 Acetohydroxyacid synthase promoter expression of introduced genes in plants

Publications (1)

Publication Number Publication Date
RO117190B1 true RO117190B1 (ro) 2001-11-30

Family

ID=23171273

Family Applications (1)

Application Number Title Priority Date Filing Date
RO97-00422A RO117190B1 (ro) 1994-09-08 1995-09-05 Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă

Country Status (26)

Country Link
US (2) US5750866A (ro)
EP (1) EP0779928B1 (ro)
JP (1) JP3655922B2 (ro)
KR (1) KR970705637A (ro)
CN (1) CN1210404C (ro)
AT (1) ATE291631T1 (ro)
AU (1) AU701239B2 (ro)
BG (1) BG64062B1 (ro)
BR (1) BR9509202A (ro)
CA (1) CA2198497C (ro)
CZ (1) CZ69597A3 (ro)
DE (1) DE69534101T2 (ro)
EE (1) EE03933B1 (ro)
ES (1) ES2240978T3 (ro)
FI (1) FI119818B (ro)
HU (1) HU221795B1 (ro)
MD (1) MD2312B2 (ro)
MX (1) MX9701684A (ro)
NO (1) NO323171B1 (ro)
NZ (1) NZ293093A (ro)
PL (1) PL188062B1 (ro)
RO (1) RO117190B1 (ro)
RU (1) RU2197527C2 (ro)
SK (1) SK30697A3 (ro)
UA (1) UA48951C2 (ro)
WO (1) WO1996007746A1 (ro)

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US6528701B1 (en) 1999-03-02 2003-03-04 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Rice ubiquitin-derived promoters
US7122721B1 (en) 1999-10-05 2006-10-17 Basf Aktiengesellschaft Plant gene expression under the control of constitutive plant V-ATPase promoters
WO2001082685A1 (en) 2000-04-28 2001-11-08 Basf Aktiengesellschaft Use of the maize x112 mutant ahas 2 gene and imidazolinone herbicides for selection of transgenic monocots, maize, rice and wheat plants resistant to the imidazolinone herbicides
EP2292768A1 (en) 2002-07-09 2011-03-09 BASF Plant Science GmbH Use of AHAS mutant genes as selection marker in potato transformation
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CA2516042A1 (en) 2003-02-17 2004-09-02 Metanomics Gmbh Preparation of transgenic plants comprising a nucleic acid molecule encoding an l450 polypeptide-exhibiting faster growth and/or increased yield
EP1641930B1 (en) 2003-04-15 2011-06-29 BASF Plant Science GmbH Nucleic acid sequences encoding proteins associated with abiotic stress response and plant cells and plants with increased tolerance to environmental stress
WO2005014828A2 (en) 2003-08-01 2005-02-17 Basf Plant Science Gmbh Process for the production of fine chemicals in plants
EP1491634A1 (en) * 2003-06-26 2004-12-29 Degussa AG Feedback resistant acetohydroxy acid synthetase mutants
EP1765857A2 (en) 2004-07-02 2007-03-28 Metanomics GmbH Process for the production of fine chemicals
AU2005268943B2 (en) 2004-07-31 2011-04-14 Metanomics Gmbh Preparation of organisms with faster growth and/or higher yield
CN103289961A (zh) 2004-09-24 2013-09-11 巴斯福植物科学有限公司 编码与非生物性胁迫反应相关的蛋白质的核酸序列和环境胁迫抗性增加的植物细胞和植物
AU2005286427B2 (en) 2004-09-24 2011-09-15 Basf Plant Science Gmbh Plant cells and plants with increased tolerance to environmental stress
CA2585798A1 (en) 2004-12-17 2006-06-17 Metanomics Gmbh Process for the control of production of fine chemicals
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US20080176225A1 (en) * 2007-01-18 2008-07-24 Steve Roffler Membrane bound reporter gene system
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US20100162432A1 (en) 2007-05-22 2010-06-24 Basf Plant Science Gmbh Plant cells and plants with increased tolerance and/or resistance to environmental stress and increased biomass production-ko
AR067318A1 (es) 2007-05-22 2009-10-07 Basf Plant Science Gmbh Plantas con mayor tolerancia y/o resistencia aumentada al estres ambiental y mayor produccion de biomasa
AU2008300579B2 (en) 2007-09-18 2014-11-13 Basf Plant Science Gmbh Plants with increased yield
EP2594646A3 (en) 2007-09-21 2013-11-06 BASF Plant Science GmbH Plant with increased yield
AU2008337398B2 (en) * 2007-12-19 2014-04-03 Basf Plant Science Gmbh Plants with increased yield and/or increased tolerance to environmental stress (IY-BM)
US20100293665A1 (en) * 2007-12-21 2010-11-18 Basf Plant Science Gmbh Plants With Increased Yield (KO NUE)
US20110010800A1 (en) * 2008-02-27 2011-01-13 Basf Plant Science Gmbh Plants with increased yield
EP2352822A2 (en) 2008-08-19 2011-08-10 BASF Plant Science GmbH Plants with increased yield by increasing or generating one or more activities in a plant or a part thereof
BRPI0919153A2 (pt) 2008-09-23 2019-05-07 Basf Plant Science Gmbh método para produzir uma planta com rendimento aumentado, molécula de ácido nucleico, construto de ácido nucleico, vetor, processo para produzir um polipeptídeo, poliptídeo, anticorpo, núcleo de célula de planta, célula de planta, tecido de planta, material de propagação, pólen, progênie, material coletado, ou uma planta, ou uma parte da planta, planta transgênica, processo para identificar um composto, método para a produção de uma composição agrícola, composição, uso de ácido nucleico, e, método para a identificação de uma planta com um rendimento aumentado, e para aumentar o rendimento de uma população de plantas
CN104745624A (zh) 2008-10-23 2015-07-01 巴斯夫植物科学有限公司 生产具有增加的γ-氨基丁酸(GABA)含量的转基因细胞的方法
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CN101475941B (zh) * 2008-11-28 2011-09-07 北京市农林科学院 一种玉米Zmptil基因启动子及其应用
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CN109468339B (zh) 2009-08-31 2022-11-04 巴斯夫植物科学有限公司 用于增强植物中组成型基因表达的调节性核酸分子
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KR20140109909A (ko) * 2011-12-30 2014-09-16 다우 아그로사이언시즈 엘엘씨 합성 양방향성 ｓｃｂｖ 식물 프로모터를 위한 방법 및 구축물
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Also Published As

Publication number Publication date
BG64062B1 (bg) 2003-11-28
BG101270A (en) 1997-10-31
HUT77109A (hu) 1998-03-02
AU701239B2 (en) 1999-01-21
FI119818B (fi) 2009-03-31
JPH10504968A (ja) 1998-05-19
EP0779928A1 (en) 1997-06-25
JP3655922B2 (ja) 2005-06-02
EP0779928B1 (en) 2005-03-23
WO1996007746A1 (en) 1996-03-14
US6025541A (en) 2000-02-15
BR9509202A (pt) 1997-12-30
ES2240978T3 (es) 2005-10-16
ATE291631T1 (de) 2005-04-15
NO971069D0 (no) 1997-03-07
NO323171B1 (no) 2007-01-15
PL319053A1 (en) 1997-07-21
PL188062B1 (pl) 2004-12-31
CA2198497C (en) 2009-11-03
DE69534101T2 (de) 2006-03-23
US5750866A (en) 1998-05-12
NO971069L (no) 1997-05-07
KR970705637A (ko) 1997-10-09
AU3544895A (en) 1996-03-27
SK30697A3 (en) 1997-12-10
HU221795B1 (hu) 2003-01-28
CZ69597A3 (en) 1997-09-17
RU2197527C2 (ru) 2003-01-27
EE03933B1 (et) 2002-12-16
FI970958L (fi) 1997-03-06
NZ293093A (en) 1999-01-28
CN1210404C (zh) 2005-07-13
CA2198497A1 (en) 1996-03-14
UA48951C2 (uk) 2002-09-16
FI970958A0 (fi) 1997-03-06
MD2312B2 (ro) 2003-11-30
MX9701684A (es) 1997-06-28
EE9700213A (et) 1998-02-16
DE69534101D1 (de) 2005-04-28
CN1157637A (zh) 1997-08-20

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