RO117190B1 - Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă - Google Patents

Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă

Info

Publication number
RO117190B1
RO117190B1 RO97-00422A RO9700422A RO117190B1 RO 117190 B1 RO117190 B1 RO 117190B1 RO 9700422 A RO9700422 A RO 9700422A RO 117190 B1 RO117190 B1 RO 117190B1
Authority
RO
Romania
Prior art keywords
plant
expression
identifying
heterologous gene
nucleic acid
Prior art date
Application number
RO97-00422A
Other languages
English (en)
Inventor
Gabriele Dietrich
Jane Smith
Jianying Peng
Original Assignee
American Cyanamid Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23171273&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=RO117190(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by American Cyanamid Company filed Critical American Cyanamid Company
Publication of RO117190B1 publication Critical patent/RO117190B1/ro

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Invenţia se referă la un construct de acid nucleic ce cuprinde un promotor AHAS pentru expresia genelor, în plante, legat operaţional la o secvenţă care codifică o genă heteroloagă, promotor care poate fi selectat dintre promotorii genelor als 1 şi als 2 de la porumb sau promotorul AHAS ce cuprinde secvenţa SEQ ID No 1, SEQ ID No 2 sau SEQ ID No 3, precum şi la o metodă de identificare a expresiei genice, într-o plantă şi/sau în diferite ţesuturi ale acesteia, şi care constă în transformarea recombinantă a unei porţiuni din plantă, expresia genei heteroloage în porţiunea din plantă, astfel transformată, şi detectarea expresiei genice.
RO97-00422A 1994-09-08 1995-09-05 Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă RO117190B1 (ro)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/303,266 US5750866A (en) 1994-09-08 1994-09-08 AHAS promoter useful for expression of introduced genes in plants
PCT/US1995/011199 WO1996007746A1 (en) 1994-09-08 1995-09-05 Acetohydroxyacid synthase promoter expression of introduced genes in plants

Publications (1)

Publication Number Publication Date
RO117190B1 true RO117190B1 (ro) 2001-11-30

Family

ID=23171273

Family Applications (1)

Application Number Title Priority Date Filing Date
RO97-00422A RO117190B1 (ro) 1994-09-08 1995-09-05 Construct de acid nucleic şi metodă pentru identificarea expresiei unei gene heteroloage, într-o plantă

Country Status (26)

Country Link
US (2) US5750866A (ro)
EP (1) EP0779928B1 (ro)
JP (1) JP3655922B2 (ro)
KR (1) KR970705637A (ro)
CN (1) CN1210404C (ro)
AT (1) ATE291631T1 (ro)
AU (1) AU701239B2 (ro)
BG (1) BG64062B1 (ro)
BR (1) BR9509202A (ro)
CA (1) CA2198497C (ro)
CZ (1) CZ69597A3 (ro)
DE (1) DE69534101T2 (ro)
EE (1) EE03933B1 (ro)
ES (1) ES2240978T3 (ro)
FI (1) FI119818B (ro)
HU (1) HU221795B1 (ro)
MD (1) MD2312B2 (ro)
MX (1) MX9701684A (ro)
NO (1) NO323171B1 (ro)
NZ (1) NZ293093A (ro)
PL (1) PL188062B1 (ro)
RO (1) RO117190B1 (ro)
RU (1) RU2197527C2 (ro)
SK (1) SK30697A3 (ro)
UA (1) UA48951C2 (ro)
WO (1) WO1996007746A1 (ro)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5659026A (en) * 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
CA2315546C (en) * 1998-02-26 2008-04-29 Pioneer Hi-Bred International, Inc. Constitutive maize promoters
EP1462522B1 (en) * 1998-02-26 2006-08-16 Pioneer Hi-Bred International, Inc. Maize Met-1 promoter
US6504083B1 (en) * 1998-10-06 2003-01-07 Pioneer Hi-Bred International, Inc. Maize Gos-2 promoters
US6528701B1 (en) 1999-03-02 2003-03-04 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Rice ubiquitin-derived promoters
US7122721B1 (en) 1999-10-05 2006-10-17 Basf Aktiengesellschaft Plant gene expression under the control of constitutive plant V-ATPase promoters
AU5920601A (en) 2000-04-28 2001-11-12 American Cyanamid Co Use of the maize x112 mutant ahas 2 gene and imidazolinone herbicides for selection of transgenic monocots, maize, rice and wheat plants resistant to the imidazolinone herbicides
EP2292768A1 (en) 2002-07-09 2011-03-09 BASF Plant Science GmbH Use of AHAS mutant genes as selection marker in potato transformation
WO2004018687A2 (en) 2002-08-07 2004-03-04 Basf Plant Science Gmbh Nucleic acid sequences encoding proteins associated with abiotic stress response
EP1597371A2 (en) 2003-02-17 2005-11-23 Metanomics GmbH Preparation of organisms with faster growth and/or higher yield
EP2194134A3 (en) 2003-04-15 2010-10-06 BASF Plant Science GmbH Nucleic acid sequences from yeast encoding proteins associated with abiotic stress response and transformed plant cells and plants with increased tolerance to environmental stress
EP1491634A1 (en) * 2003-06-26 2004-12-29 Degussa AG Feedback resistant acetohydroxy acid synthetase mutants
AU2004262656A1 (en) 2003-08-01 2005-02-17 Basf Plant Science Gmbh Process for the production of fine chemicals in plants
AU2005321630A1 (en) 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
WO2007087815A2 (en) 2004-12-17 2007-08-09 Metanomics Gmbh Process for the control of production of fine chemicals
JP2008507960A (ja) 2004-07-31 2008-03-21 メタノミクス ゲーエムベーハー より成長が早いかつ／又はより収量が高い生物の作製
CN101495507B (zh) 2004-09-24 2013-07-17 巴斯福植物科学有限公司 编码与非生物性胁迫反应相关的蛋白质的核酸序列和具有增加的环境胁迫抗性的植物细胞和植物
CA2579927A1 (en) 2004-09-24 2006-03-30 Basf Plant Science Gmbh Plant cells and plants with increased tolerance to environmental stress
EP2573188A2 (en) 2005-03-02 2013-03-27 Metanomics GmbH Process for the production of fine chemicals
CA2598792A1 (en) * 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US7737326B2 (en) * 2005-04-29 2010-06-15 Midwest Oil Seeds Inc. EPSPS promoter from maize
EP2333078A3 (en) 2006-03-24 2012-01-04 BASF Plant Science GmbH Proteins associated with abiotic stress response and homologs
EP2090662A3 (en) 2006-04-05 2012-10-31 Metanomics GmbH Process for the production of a fine chemical
US20090300794A1 (en) 2006-05-31 2009-12-03 Metanomics Gmbh Manipulation Of The Nitrogen Metabolism
US8344205B2 (en) 2006-10-13 2013-01-01 Basf Plant Science Gmbh Plants with increased yield
US20080176225A1 (en) * 2007-01-18 2008-07-24 Steve Roffler Membrane bound reporter gene system
CA2682349C (en) 2007-04-04 2017-08-22 Basf Plant Science Gmbh Ahas mutants
AU2008252998A1 (en) 2007-05-22 2008-11-27 Basf Plant Science Gmbh Plant cells and plants with increased tolerance and/or resistance to environmental stress and increased biomass production-KO
EP2064330A2 (en) 2007-05-22 2009-06-03 BASF Plant Science GmbH Plants with increased tolerance and/or resistance to environmental stress and increased biomass production
US8664475B2 (en) 2007-09-18 2014-03-04 Basf Plant Science Gmbh Plants with increased yield
DE112008002456T5 (de) 2007-09-21 2011-01-13 Basf Plant Science Gmbh Pflanzen mit erhöhtem Ertrag
WO2009077611A2 (en) 2007-12-19 2009-06-25 Basf Plant Science Gmbh Plants with increased yield and/or increased tolerance to environmental stress (iy-bm)
CN101952444A (zh) * 2007-12-21 2011-01-19 巴斯夫植物科学有限公司 产量提高的植物(ko nue)
AR070719A1 (es) * 2008-02-27 2010-04-28 Basf Plant Science Gmbh Plantas con mayor rendimiento, en terminos de crecimiento, desarrollo, acumulacion de biomasas y generacion de semillas en comparacion con una planta silvestre, y un metodo para producirlas.
EP2352822A2 (en) 2008-08-19 2011-08-10 BASF Plant Science GmbH Plants with increased yield by increasing or generating one or more activities in a plant or a part thereof
BRPI0919153A2 (pt) 2008-09-23 2019-05-07 Basf Plant Science Gmbh método para produzir uma planta com rendimento aumentado, molécula de ácido nucleico, construto de ácido nucleico, vetor, processo para produzir um polipeptídeo, poliptídeo, anticorpo, núcleo de célula de planta, célula de planta, tecido de planta, material de propagação, pólen, progênie, material coletado, ou uma planta, ou uma parte da planta, planta transgênica, processo para identificar um composto, método para a produção de uma composição agrícola, composição, uso de ácido nucleico, e, método para a identificação de uma planta com um rendimento aumentado, e para aumentar o rendimento de uma população de plantas
CA2740257A1 (en) 2008-10-23 2010-04-29 Basf Plant Science Gmbh Plants with increased yield (nue)
BRPI0924536A2 (pt) 2008-10-23 2015-08-11 Basf Plant Science Gmbh Método para produzir uma célula transgênica com teor de ácido gama-aminobutírico (gaba) aumentado, para aumentar o rendimento, molécula de ácido nucleico isolada, construção de ácido nucleico, vetor, célula hospedeira, processo para produzir um polipeptídeo, polipeptídeo, anticorpo, e, núcleo de célula, célula, núcleo de célula vegetal, célula vegetal, tecido vegetal, material de propagação, pólen, progênie, material coletado ou uma planta
CN101475941B (zh) * 2008-11-28 2011-09-07 北京市农林科学院 一种玉米Zmptil基因启动子及其应用
WO2011009801A1 (en) 2009-07-23 2011-01-27 Basf Plant Science Company Gmbh Plants with increased yield
WO2011023537A1 (en) 2009-08-31 2011-03-03 Basf Plant Science Company Gmbh Regulatory nucleic acid molecules for enhancing constitutive gene expression in plants
US9428757B2 (en) 2009-08-31 2016-08-30 Basf Plant Science Company Gmbh Regulatory nucleic acid molecules for enhancing seed-specific gene expression in plants promoting enhanced polyunsaturated fatty acid synthesis
SG178388A1 (en) * 2009-08-31 2012-03-29 Basf Plant Science Co Gmbh Regulatory nucleic acid molecules for enhancing seed-specific and/or seed-preferential gene expression in plants
EP2319872A1 (en) 2009-11-04 2011-05-11 BASF Plant Science GmbH Amylopectin type starch with enhanced retrogradation stability
CA2780707A1 (en) 2009-11-17 2011-05-26 Basf Plant Science Company Gmbh Plants with increased yield
EP2797406B1 (en) * 2011-12-30 2020-01-08 Dow AgroSciences LLC Construct and method for synthetic bidirectional plant promoter ubi1
EP2797407A4 (en) * 2011-12-30 2015-07-08 Dow Agrosciences Llc METHOD AND CONSTRUCTION FOR SYNTHETIC BIDIRECTIONAL SCBV PLANT PROMOTER
CN102676549B (zh) * 2012-01-09 2014-04-09 中国中医科学院中药研究所 一个参与丹参酮生物合成的cyp450基因及其编码产物与应用
WO2013163613A2 (en) * 2012-04-27 2013-10-31 Synthetic Genomics, Inc. Compositions and methods for modulating the sensitivity of cells to ahas inhibitors
US10231397B2 (en) 2013-01-29 2019-03-19 Basf Plant Science Company Gmbh Fungal resistant plants expressing EIN2
US10462994B2 (en) 2013-01-29 2019-11-05 Basf Plant Science Company Gmbh Fungal resistant plants expressing HCP7
CA2897485A1 (en) 2013-01-29 2014-08-07 Basf Plant Science Company Gmbh Fungal resistant plants expressing hcp6
CA2900005A1 (en) 2013-03-08 2014-09-12 Basf Plant Science Company Gmbh Fungal resistant plants expressing mybtf
CR20200121A (es) 2013-03-14 2020-07-03 Monsanto Technology Llc Elementos regulatorios de planta y sus usos (divisional 2015-0554)
BR112017016688B1 (pt) 2015-02-04 2024-01-23 Basf Plant Science Company Gmbh Método para aumentar a resistência fúngica, método para a produção de um produto e método para criar uma planta resistente a fungos
EP3054014A3 (en) 2016-05-10 2016-11-23 BASF Plant Science Company GmbH Use of a fungicide on transgenic plants

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4943674A (en) * 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US5013659A (en) * 1987-07-27 1991-05-07 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
EP0360750A3 (en) * 1988-09-22 1991-01-02 Ciba-Geigy Ag Novel herbicide tolerant plants
ES2104628T3 (es) * 1990-05-09 1997-10-16 American Cyanamid Co Metodo para evitar daños a los cultivos en presencia de combinaciones pesticidas sinergeticas.
GB9024728D0 (en) * 1990-11-14 1991-01-02 Ici Plc Herbicide resistant plants
TW208716B (ro) * 1990-12-27 1993-07-01 American Cyanamid Co
US5731180A (en) * 1991-07-31 1998-03-24 American Cyanamid Company Imidazolinone resistant AHAS mutants

Also Published As

Publication number Publication date
WO1996007746A1 (en) 1996-03-14
EE03933B1 (et) 2002-12-16
CA2198497A1 (en) 1996-03-14
AU3544895A (en) 1996-03-27
EP0779928B1 (en) 2005-03-23
ES2240978T3 (es) 2005-10-16
EE9700213A (et) 1998-02-16
RU2197527C2 (ru) 2003-01-27
CZ69597A3 (en) 1997-09-17
NO971069D0 (no) 1997-03-07
NZ293093A (en) 1999-01-28
CA2198497C (en) 2009-11-03
PL319053A1 (en) 1997-07-21
US6025541A (en) 2000-02-15
DE69534101D1 (de) 2005-04-28
MD2312B2 (ro) 2003-11-30
BG64062B1 (bg) 2003-11-28
HUT77109A (hu) 1998-03-02
FI119818B (fi) 2009-03-31
NO323171B1 (no) 2007-01-15
UA48951C2 (uk) 2002-09-16
FI970958A0 (fi) 1997-03-06
ATE291631T1 (de) 2005-04-15
AU701239B2 (en) 1999-01-21
FI970958L (fi) 1997-03-06
EP0779928A1 (en) 1997-06-25
NO971069L (no) 1997-05-07
BR9509202A (pt) 1997-12-30
BG101270A (en) 1997-10-31
JP3655922B2 (ja) 2005-06-02
US5750866A (en) 1998-05-12
PL188062B1 (pl) 2004-12-31
CN1157637A (zh) 1997-08-20
JPH10504968A (ja) 1998-05-19
KR970705637A (ko) 1997-10-09
MX9701684A (es) 1997-06-28
SK30697A3 (en) 1997-12-10
HU221795B1 (hu) 2003-01-28
DE69534101T2 (de) 2006-03-23
CN1210404C (zh) 2005-07-13

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