CA1300532C - Conjugat covalent oligonucleotide-peroxydase du raifort - Google Patents

Conjugat covalent oligonucleotide-peroxydase du raifort

Info

Publication number: CA1300532C
Authority: CA; Canada
Prior art keywords: oligonucleotide; hrp; mmole; conjugate; oligonucleotide chain
Prior art date: 1987-10-02
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA000578385A

Other languages

English (en)

Inventor

Fred T. Oaks

Corey Levenson

Chu-An Chang

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Novartis Vaccines and Diagnostics Inc

Original Assignee

Cetus Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-10-02

Filing date

1988-09-26

Publication date

1992-05-12

1988-09-26 Application filed by Cetus Corp filed Critical Cetus Corp

1992-05-12 Application granted granted Critical

1992-05-12 Publication of CA1300532C publication Critical patent/CA1300532C/fr

2009-05-12 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

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239000010452 phosphate Substances 0.000 description 1
229920001451 polypropylene glycol Polymers 0.000 description 1
XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
239000013014 purified material Substances 0.000 description 1
UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
239000000741 silica gel Substances 0.000 description 1
229910002027 silica gel Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000007858 starting material Substances 0.000 description 1
239000000126 substance Substances 0.000 description 1
235000011149 sulphuric acid Nutrition 0.000 description 1
150000003573 thiols Chemical class 0.000 description 1
238000000870 ultraviolet spectroscopy Methods 0.000 description 1
238000005303 weighing Methods 0.000 description 1
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical class OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
Saccharide Compounds (AREA)

CA000578385A 1987-10-02 1988-09-26 Conjugat covalent oligonucleotide-peroxydase du raifort Expired - Fee Related CA1300532C (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US10397887A	1987-10-02	1987-10-02
US103,978		1987-10-02

Publications (1)

Publication Number	Publication Date
CA1300532C true CA1300532C (fr)	1992-05-12

Family

ID=22298041

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000578385A Expired - Fee Related CA1300532C (fr)	1987-10-02	1988-09-26	Conjugat covalent oligonucleotide-peroxydase du raifort

Country Status (5)

Country	Link
EP (1)	EP0372002A1 (fr)
JP (1)	JPH0630574B2 (fr)
CA (1)	CA1300532C (fr)
IL (1)	IL87880A (fr)
WO (1)	WO1989002932A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE69012109T2 (de) *	1989-03-10	1995-01-26	Millipore Corp	Dns-sequenzierung mit verwendung von chemolumineszenz.
US5272077A (en) *	1989-09-12	1993-12-21	Eastman Kodak Company	Method for preparing a covalent conjugate of an oligonucleotide and an enzyme
US5082780A (en) *	1989-09-12	1992-01-21	Eastman Kodak Company	Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures
CA2032331A1 (fr) *	1990-01-22	1991-07-23	Annie L. Wu	Methode et trousse pour detecter l'adn de l'antigene des leucocytes humains
WO1992000989A1 (fr) *	1990-07-10	1992-01-23	Imperial Chemical Industries Plc	Procede de marquage non-isotopique d'acide nucleique
US5633230A (en) *	1990-10-24	1997-05-27	Allelix Biopharmaceuticals, Inc.	Treatment of cytomegalovirus infection
US5474895A (en) *	1990-11-14	1995-12-12	Siska Diagnostics Inc.	Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
US5495006A (en) *	1991-09-27	1996-02-27	Allelix Biopharmaceuticals, Inc.	Antiviral polynucleotide conjugates
WO2017097973A1 (fr) *	2015-12-09	2017-06-15	Universitaet Konstanz	Nucléosides modifiés

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB8306426D0 (en) *	1983-03-09	1983-04-13	Malcolm A D B	Detecting polynucleotide sequence
US4626501A (en) *	1983-09-02	1986-12-02	Integrated Genetics, Inc.	Labeled DNA
US4657853A (en) *	1984-09-14	1987-04-14	E. I. Du Pont De Nemours And Company	Immunoassays utilizing covalent conjugates of polymerized enzyme and antibody
GB8509880D0 (en) *	1985-04-17	1985-05-22	Ici Plc	Testing device

1988
- 1988-09-19 WO PCT/US1988/003213 patent/WO1989002932A1/fr not_active Ceased
- 1988-09-19 EP EP88908882A patent/EP0372002A1/fr not_active Withdrawn
- 1988-09-19 JP JP63508221A patent/JPH0630574B2/ja not_active Expired - Fee Related
- 1988-09-26 CA CA000578385A patent/CA1300532C/fr not_active Expired - Fee Related
- 1988-09-29 IL IL87880A patent/IL87880A/xx not_active IP Right Cessation

Also Published As

Publication number	Publication date
EP0372002A1 (fr)	1990-06-13
JPH02502695A (ja)	1990-08-30
IL87880A (en)	1993-08-18
WO1989002932A1 (fr)	1989-04-06
JPH0630574B2 (ja)	1994-04-27
IL87880A0 (en)	1989-03-31

Legal Events

Date	Code	Title	Description
1999-05-12	MKLA	Lapsed

Publication	Publication Date	Title
EP0380559B1 (fr)	1993-12-22	Procedes et reactifs de fonctionnalisation d'oligonucleotides
US4962029A (en)	1990-10-09	Covalent oligonucleotide-horseradish peroxidase conjugate
Sinha et al.	1988	The preparation and application of functionalised synthetic oligonucleotides: III. Use of H-phosphonate derivatives of protected amino-hexanol and mercapto-propanol or-hexanol
KR920007274B1 (ko)	1992-08-29	변형된 포스포라미디트를 이용하는 변형 핵산의 제조방법
Gupta et al.	1991	A general method for the synthesis of 3′-sulfhydryl and phosphate group containing oligonucleotides
Haralambidis et al.	1987	Preparation of base-modified nucleosides suitable for non-radioactive label attachment and their incorporation into synthetic oligodeoxyribonucleotides
US4849336A (en)	1989-07-18	Oligonucleotide derivatives and production thereof
US5321131A (en)	1994-06-14	Site-specific functionalization of oligodeoxynucleotides for non-radioactive labelling
EP0101985B1 (fr)	1987-09-23	Dérivés d'oligonucléotides et leur procédé de préparation
US5371241A (en)	1994-12-06	Fluorescein labelled phosphoramidites
EP0261283B1 (fr)	1992-01-15	Agents de liaison à base de phosphites et phosphates aiyant des fonctions amino, produits liés à ceux-ci et précurseurs phosphoramiditiques
US5367066A (en)	1994-11-22	Oligonucleotides with selectably cleavable and/or abasic sites
US8183355B2 (en)	2012-05-22	Method for the synthesis of oligonucleotide derivatives
EP0451213B1 (fr)	1994-04-06	Derives de molecules polyhydroxylees permettant l'introduction d'au moins une ramification dans un oligonucleotide
FR2596761A1 (fr)	1987-10-09	Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides
FR2575751A1 (fr)	1986-07-11	Nouveaux nucleosides de derives de l'adenosine, leur preparation et leurs applications biologiques
JPH02504144A (ja)	1990-11-29	ヌクレオシド誘導体
CA1300532C (fr)	1992-05-12	Conjugat covalent oligonucleotide-peroxydase du raifort
US5258538A (en)	1993-11-02	2,3-disubstituted-1,3,2-oxazaphosphacycloalkanes as nucleic acid linking agents
Grzybowski et al.	1993	Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2, 4-dinitrophenyl reporter groups
US6013434A (en)	2000-01-11	Oligonucleotide-polyamide conjugates
Katti et al.	1986	Separation of diastereomers of methylphosphonate dinucleotides
AU4054693A (en)	1993-12-30	Oligonucleotide-polyamide conjugates
JPWO1988003149A1 (ja)	1988-10-06	オリゴヌクレオチドの合成法および高分子保護基形成用化合物
JPH07505907A (ja)	1995-06-29	オリゴヌクレオチド−ポリアミドコンジュゲート