CR20120560A - PRODUCCIÓN DE TNFR-Ig Y FUSIÓN DE PROTEÍNA - Google Patents

PRODUCCIÓN DE TNFR-Ig Y FUSIÓN DE PROTEÍNA

Info

Publication number
CR20120560A
CR20120560A CR20120560A CR20120560A CR20120560A CR 20120560 A CR20120560 A CR 20120560A CR 20120560 A CR20120560 A CR 20120560A CR 20120560 A CR20120560 A CR 20120560A CR 20120560 A CR20120560 A CR 20120560A
Authority
CR
Costa Rica
Prior art keywords
cumulative
amino acid
glutamine
accumulated
approximately
Prior art date
Application number
CR20120560A
Other languages
English (en)
Inventor
Denis Drapeau
Yen-Tuang Luan
James R Mercer
Wenge Wang
Daniel Lasko
Original Assignee
Pfizer Ireland Pharmaceuticals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=35500648&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CR20120560(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Pfizer Ireland Pharmaceuticals filed Critical Pfizer Ireland Pharmaceuticals
Publication of CR20120560A publication Critical patent/CR20120560A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La invención presente proporciona un sistema mejorado para la producción en gran escala de proteínas o polipéptidos en cultivo celular particularmente en un medio caracterizado por uno o más de: i) una cantidad de aminoácido acumulado por unidad de volumen mayor de unos 70 mM; ii) una cantidad molar acumuladade glutamina acumulativa a la asparagina de menos de 2; III) una cantidad molar acumulada de glutamina acumulativa al aminoácido total acumulativo de menos de aproximadamente 0,2; IV) un molar ion inorgánico acumulativo a aminoácido total acumulativo relación entre aproximadamente 0.4 a 1; o v) una suma acumulativa de glutamina y asparagina concentración por unidad de volumen mayor de entre aproximadamente 16 mM y 36 mM según se proporciona.
CR20120560A 2004-08-27 2012-11-01 PRODUCCIÓN DE TNFR-Ig Y FUSIÓN DE PROTEÍNA CR20120560A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US60537904P 2004-08-27 2004-08-27

Publications (1)

Publication Number Publication Date
CR20120560A true CR20120560A (es) 2012-12-04

Family

ID=35500648

Family Applications (2)

Application Number Title Priority Date Filing Date
CR8998A CR8998A (es) 2004-08-27 2007-03-16 PRODUCCION DE TNFR-Ig Y FUSION DE PROTEINA
CR20120560A CR20120560A (es) 2004-08-27 2012-11-01 PRODUCCIÓN DE TNFR-Ig Y FUSIÓN DE PROTEÍNA

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CR8998A CR8998A (es) 2004-08-27 2007-03-16 PRODUCCION DE TNFR-Ig Y FUSION DE PROTEINA

Country Status (37)

Country Link
US (1) US7300773B2 (es)
EP (2) EP1992697B1 (es)
JP (3) JP2008511330A (es)
KR (1) KR100988451B1 (es)
CN (2) CN101061231B (es)
AR (2) AR050537A1 (es)
AT (2) ATE446376T1 (es)
AU (1) AU2005280036B2 (es)
BR (1) BRPI0514694B8 (es)
CA (1) CA2578138C (es)
CL (1) CL2017000577A1 (es)
CR (2) CR8998A (es)
CY (2) CY1109721T1 (es)
DE (2) DE602005020076D1 (es)
DK (2) DK1992697T3 (es)
EC (1) ECSP077354A (es)
EG (1) EG26922A (es)
ES (2) ES2341390T3 (es)
GT (2) GT200500233A (es)
HN (1) HN2005000485A (es)
HR (2) HRP20100011T1 (es)
IL (1) IL181588A (es)
MX (1) MX2007002381A (es)
MY (1) MY137803A (es)
NO (1) NO344785B1 (es)
NZ (1) NZ579208A (es)
PE (2) PE20100448A1 (es)
PL (2) PL1781802T3 (es)
PT (2) PT1992697E (es)
RS (2) RS51255B (es)
RU (1) RU2458988C2 (es)
SI (2) SI1992697T1 (es)
SV (1) SV2006002211A (es)
TW (1) TWI364458B (es)
UA (1) UA89383C2 (es)
WO (1) WO2006026447A2 (es)
ZA (1) ZA200701672B (es)

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Also Published As

Publication number Publication date
HK1121497A1 (en) 2009-04-24
ECSP077354A (es) 2007-05-30
ES2341390T3 (es) 2010-06-18
UA89383C2 (ru) 2010-01-25
CR8998A (es) 2007-11-23
EP1781802B1 (en) 2009-10-21
HN2005000485A (es) 2009-06-09
HK1099941A1 (en) 2007-08-31
DE602005020076D1 (de) 2010-04-29
WO2006026447A3 (en) 2006-04-20
BRPI0514694B1 (pt) 2019-04-24
NZ579208A (en) 2012-01-12
IL181588A0 (en) 2007-07-04
BRPI0514694A (pt) 2008-06-17
PL1992697T3 (pl) 2010-08-31
HRP20100011T1 (hr) 2010-02-28
US20060121569A1 (en) 2006-06-08
CY1110092T1 (el) 2015-01-14
ZA200701672B (en) 2012-04-24
EG26922A (en) 2014-12-25
TW200619388A (en) 2006-06-16
DK1992697T3 (da) 2010-05-17
KR100988451B1 (ko) 2010-10-18
WO2006026447A2 (en) 2006-03-09
GT200500234A (es) 2006-10-10
HRP20100271T1 (hr) 2010-06-30
CL2017000577A1 (es) 2017-12-01
GT200500233A (es) 2006-03-09
PE20060815A1 (es) 2006-09-14
RU2458988C2 (ru) 2012-08-20
PT1781802E (pt) 2010-01-04
TWI364458B (en) 2012-05-21
NO344785B1 (no) 2020-04-27
CA2578138A1 (en) 2006-03-09
RS51072B (sr) 2010-10-31
JP2012095672A (ja) 2012-05-24
CN101061231B (zh) 2012-09-26
IL181588A (en) 2010-12-30
CA2578138C (en) 2010-10-05
AU2005280036A1 (en) 2006-03-09
SI1992697T1 (sl) 2010-07-30
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AU2005280036B2 (en) 2011-11-03
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JP5921910B2 (ja) 2016-05-24
ATE446376T1 (de) 2009-11-15
CN102876761A (zh) 2013-01-16
SV2006002211A (es) 2006-10-04
PT1992697E (pt) 2010-04-21
EP1781802A2 (en) 2007-05-09
ATE461285T1 (de) 2010-04-15
ES2335518T3 (es) 2010-03-29
RS51255B (sr) 2010-12-31
US7300773B2 (en) 2007-11-27
PL1781802T3 (pl) 2010-03-31
EP1992697A1 (en) 2008-11-19
BRPI0514694B8 (pt) 2021-05-25
AR050537A1 (es) 2006-11-01
CN101061231A (zh) 2007-10-24
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AR088824A2 (es) 2014-07-10
JP2008511330A (ja) 2008-04-17
DK1781802T3 (da) 2010-01-25
CY1109721T1 (el) 2014-08-13
RU2007108717A (ru) 2008-10-10
EP1992697B1 (en) 2010-03-17
KR20070069140A (ko) 2007-07-02
WO2006026447A9 (en) 2006-06-01
JP2016056214A (ja) 2016-04-21
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