DD210306A5 - Verfahren zur herstellung eines rekombinanten dna-klonierungsvektors zur expression von exogenem protein - Google Patents

Verfahren zur herstellung eines rekombinanten dna-klonierungsvektors zur expression von exogenem protein Download PDF

Info

Publication number: DD210306A5
Authority: DD; German Democratic Republic
Prior art keywords: fragment; sequence; dna; plasmid; asp
Prior art date: 1982-05-25

Application number

DD25121483A

Other languages

German (de)

English (en)

Inventor

Nancy G Mayne

James P Burnett Jr

Ramamoorthy Belagaje

Hansen M Hsiung

Original Assignee

Lilly Co Eli

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1982-05-25

Filing date

1983-05-24

Publication date

1984-06-06

1983-05-24 Application filed by Lilly Co Eli filed Critical Lilly Co Eli

1984-06-06 Publication of DD210306A5 publication Critical patent/DD210306A5/de

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125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
239000013014 purified material Substances 0.000 description 1
101150079601 recA gene Proteins 0.000 description 1
238000011084 recovery Methods 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
231100000935 short-term exposure limit Toxicity 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000007790 solid phase Substances 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
238000011426 transformation method Methods 0.000 description 1
230000001131 transforming effect Effects 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
238000010792 warming Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Biomedical Technology (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Biophysics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Endocrinology (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Saccharide Compounds (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)

DD25121483A 1982-05-25 1983-05-24 Verfahren zur herstellung eines rekombinanten dna-klonierungsvektors zur expression von exogenem protein DD210306A5 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US38205182A	1982-05-25	1982-05-25
US38199282A	1982-05-25	1982-05-25

Publications (1)

Publication Number	Publication Date
DD210306A5 true DD210306A5 (de)	1984-06-06

Family

ID=27009591

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD25121483A DD210306A5 (de)	1982-05-25	1983-05-24	Verfahren zur herstellung eines rekombinanten dna-klonierungsvektors zur expression von exogenem protein

Country Status (10)

Country	Link
EP (1)	EP0095361B1 (da)
JP (2)	JPH0759193B2 (da)
AU (1)	AU560965B2 (da)
CA (1)	CA1231068A (da)
DD (1)	DD210306A5 (da)
DE (1)	DE3380262D1 (da)
DK (1)	DK230683A (da)
GB (1)	GB2121054B (da)
HU (1)	HU197349B (da)
IE (1)	IE55244B1 (da)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
NZ201918A (en) *	1981-09-18	1987-04-30	Genentech Inc	N-terminal methionyl analogues of bovine growth hormone
EP0111814A3 (en) *	1982-12-16	1985-08-14	Mgi Pharma, Inc.	The cloning and expression of nucleotide sequences encoding bovine growth hormone
US4755465A (en) *	1983-04-25	1988-07-05	Genentech, Inc.	Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US4859600A (en) *	1983-04-25	1989-08-22	Genentech, Inc.	Recombinant procaryotic cell containing correctly processed human growth hormone
JP2551746B2 (ja) *	1983-07-19	1996-11-06	サントリー株式会社	改良プラスミドベクタ−およびその利用
DOP1985004337A (es) *	1984-03-06	1990-09-17	Lilly Co Eli	Vectores y metodo para expresion genetica
EP0159123B1 (en) *	1984-03-06	1992-01-15	Eli Lilly And Company	Vectors for expressing bovine growth hormone derivatives
US4745179A (en) *	1984-04-02	1988-05-17	Fujisawa Pharmaceutical Co., Ltd.	59 Valine insulin-like growth factor I and process for production thereof
GB8412517D0 (en) *	1984-05-16	1984-06-20	Nagai K	Recombinant fusion proteins
US4861868A (en)	1985-02-22	1989-08-29	Monsanto Company	Production of proteins in procaryotes
US5037806A (en)	1985-02-22	1991-08-06	Monsanto Company	Biologically active method using somatotropins
JPS61206908A (ja) *	1985-03-08	1986-09-13	Sony Corp	磁気デイスク装置
US5342921A (en) *	1985-03-28	1994-08-30	Chiron Corporation	Superoxide dismutase fusion polypeptides for expression of mammalian proteins
US4751180A (en) *	1985-03-28	1988-06-14	Chiron Corporation	Expression using fused genes providing for protein product
IL78703A (en) *	1985-05-10	1993-02-21	Benzon Alfred	Method of producing extracellular enzymes, the enzymes produced thereby and compositions containing them; a hybrid plasmid and a dna fragment comprising dna encoding the enzymes; a microorganism harbouring the plasmid; and a method for removing nucleic acids from biological materials
US5173418A (en) *	1985-05-10	1992-12-22	Benzon Pharma, A/S	Production in Escherichia coli of extracellular Serratia spp. hydrolases
DE3731874A1 (de) *	1987-09-18	1989-03-30	Schering Ag	Verfahren zur herstellung von peptiden durch spezifische spaltung von gentechnisch gewonnenen fusionsproteinen mit collagenasen
US5063158A (en) *	1987-11-09	1991-11-05	Eli Lilly And Company	Recombinant DNA expression vector comprising both transcriptional and translational activating sequences
US5192669A (en) *	1987-11-09	1993-03-09	Eli Lilly And Company	Method to produce recombinant proteins using an expression vector comprising transcriptional and translational activating sequences
IL90514A (en) *	1988-06-03	1995-10-31	Us Health	A fusion gene that contains a gene for multidrug resistance that is fused to a gene for adenine diamines
DE68928124T2 (de)	1988-09-02	1997-10-16	Chiron Corp., Emeryville, Calif.	Makrophagenabgeleiteter entzündungsmediator(mip-2)
EP0752853B1 (en)	1993-03-10	2005-09-21	Smithkline Beecham Corporation	Human brain phosphodiesterase
US5795776A (en) *	1994-03-22	1998-08-18	Bio-Technology General Corp.	Expression plasmids regulated by an OSMB promoter
AU7171098A (en) *	1997-05-01	1998-11-24	Board Of Regents, The University Of Texas System	Directed evolution of enzymes and antibodies
US6087128A (en)	1998-02-12	2000-07-11	Ndsu Research Foundation	DNA encoding an avian E. coli iss
EP1088888A4 (en) *	1998-05-14	2005-03-16	Merck Patent Gmbh	MERGED PROTEIN
DE102005014270A1 (de) *	2005-03-24	2006-10-05	Rhein Chemie Rheinau Gmbh	Verwendung von vernetzten Mikrogelen zur Modifikation des temperaturabhängigen Verhaltens von nicht-vernetzbaren organischen Medien
WO2016176573A1 (en)	2015-04-30	2016-11-03	Board Of Regents, The University Of Texas System	Antibacterial polypeptide libraries and methods for screening the same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
NO158880C (no) *	1977-11-08	1988-11-09	Genentech Inc	Fremgangsmaate for fremstilling av et spesifikt pattedyrhormon.
GR68404B (da) *	1979-06-01	1981-12-29	Univ California
US4342832A (en) *	1979-07-05	1982-08-03	Genentech, Inc.	Method of constructing a replicable cloning vehicle having quasi-synthetic genes
CA1200773A (en) *	1980-02-29	1986-02-18	William J. Rutter	Expression linkers
IE52755B1 (en) *	1980-08-26	1988-02-17	Univ California	Bovine pre-growth and growth hormone
GR76959B (da) *	1981-01-02	1984-09-04	Univ New York State Res Found
NZ201918A (en) *	1981-09-18	1987-04-30	Genentech Inc	N-terminal methionyl analogues of bovine growth hormone

1983
- 1983-05-23 IE IE121183A patent/IE55244B1/en not_active IP Right Cessation
- 1983-05-23 DE DE8383302935T patent/DE3380262D1/de not_active Expired
- 1983-05-23 HU HU181083A patent/HU197349B/hu not_active IP Right Cessation
- 1983-05-23 GB GB08314183A patent/GB2121054B/en not_active Expired
- 1983-05-23 EP EP83302935A patent/EP0095361B1/en not_active Expired
- 1983-05-24 CA CA000428700A patent/CA1231068A/en not_active Expired
- 1983-05-24 DD DD25121483A patent/DD210306A5/de unknown
- 1983-05-24 DK DK230683A patent/DK230683A/da not_active Application Discontinuation
- 1983-05-24 JP JP58092197A patent/JPH0759193B2/ja not_active Expired - Lifetime
- 1983-05-24 AU AU14912/83A patent/AU560965B2/en not_active Ceased
1992
- 1992-09-17 JP JP4351893A patent/JPH0673096A/ja active Pending

Also Published As

Publication number	Publication date
JPH0673096A (ja)	1994-03-15
AU1491283A (en)	1983-12-01
DE3380262D1 (en)	1989-08-31
GB2121054B (en)	1986-02-26
HU197349B (en)	1989-03-28
CA1231068A (en)	1988-01-05
JPH0759193B2 (ja)	1995-06-28
EP0095361B1 (en)	1989-07-26
DK230683D0 (da)	1983-05-24
AU560965B2 (en)	1987-04-30
DK230683A (da)	1983-11-26
IE831211L (en)	1983-11-25
EP0095361A1 (en)	1983-11-30
GB8314183D0 (en)	1983-06-29
IE55244B1 (en)	1990-07-18
JPS58219199A (ja)	1983-12-20
GB2121054A (en)	1983-12-14

Legal Events

Date	Code	Title	Description
1984-06-06	A5	Published as prov. exclusive patent

Publication	Publication Date	Title
DD210306A5 (de)	1984-06-06	Verfahren zur herstellung eines rekombinanten dna-klonierungsvektors zur expression von exogenem protein
DE3586750T2 (de)	1993-04-22	Rekombinante fusionsproteine.
DE3280471T2 (de)	1996-04-04	Mikrobisch-produziertes Rinderwachstumshormon und dessen Verwendung.
DE3486216T2 (de)	1994-02-17	Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren.
DE69521752T2 (de)	2001-11-08	Verfahren für die Herstellung von Proteinen
DE69233008T2 (de)	2004-01-22	Fusionierung von Peptiden und Proteinen mit Thioredoxin und thioredoxin-ähnlichen Molekülen
DE60208343T2 (de)	2006-06-22	FUSIONSPROTEINE aus hirudin und proinsulin oder insulin
DE69032743T2 (de)	1999-06-17	Herstellung von fusionsproteinen
US4745069A (en)	1988-05-17	Cloning vectors for expression of exogenous protein
DD157343A5 (de)	1982-11-03	Mikrobielle expression von quasi-synthetischen genen
DE2848053A1 (de)	1979-05-10	Synthetische dna und verfahren zu ihrer herstellung
DE3787820T2 (de)	1994-04-28	Für Flagellin kodierende DNS und dieses enthaltender Vektor.
DE2848051A1 (de)	1979-05-10	Synthetische dna und verfahren zu ihrer herstellung
DE2848052A1 (de)	1979-05-10	Synthetische dna und verfahren zu ihrer herstellung
DD212982A5 (de)	1984-08-29	Verfahren zur herstellung von rinder-wachstumshormon-aehnlichen polypeptiden
EP0545999B1 (en)	1997-06-04	Process for the preparation of biologically active IGF-1 using IGF-1 having an amino-terminal extension
EP1114865B1 (en)	2004-01-21	Production of human parathyroid hormone
DE69010096T2 (de)	1994-10-13	Expression und Sekretion von reifem menschlichem beta-Interleukin-1 in Bacillus subtilis und Mittel und Verfahren zu deren Ausführung.
DE69735242T2 (de)	2006-09-21	N-terminal verlängerte proteine exprimiert in hefe
JP3319605B2 (ja)	2002-09-03	ストレプトミセスにおける外来タン白質の製造法
EP0147178B1 (en)	1991-08-14	Expression plasmids for improved production of heterologous protein in bacteria
DE3887856T2 (de)	1994-05-19	Methode zur Herstellung von natürlichen, menschlichen Wachstumshormon in reiner Form.
DD298426A5 (de)	1992-02-20	Verfahren zur herstellung von neuen plasmiden und deren verwendung bei der gewinnung eines plasminogenaktivators
DE3587205T2 (de)	1993-08-26	Verfahren zur herstellung eines proteins und dafuer zu verwendender vektor, rekombinante dns und transformierte zelle.
DE3485923T2 (de)	1993-03-04	Dna-gen, verfahren zu seiner herstellung und dieses gen enthaltendes plasmid.