DD270723A5 - Verfahren zur Herstellung von hochreinem Chymopapain - Google Patents

Verfahren zur Herstellung von hochreinem Chymopapain Download PDF

Info

Publication number: DD270723A5
Authority: DD; German Democratic Republic
Prior art keywords: chymopapain; buffer; preparation; crude; reducing agent
Prior art date: 1986-12-08

Application number

DD31628488A

Other languages

German (de)

English (en)

Inventor

Laszlo Polgar

Jozsef Gaal

Sandor Virag

Laszlo Jozsa

Tibor Vizkeleti

Bence Asboth

Erzsebet Fejer

Original Assignee

Chinoin Gyogyszer Es Vegyeszet

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-12-08

Filing date

1988-06-01

Publication date

1989-08-09

1988-06-01 Application filed by Chinoin Gyogyszer Es Vegyeszet filed Critical Chinoin Gyogyszer Es Vegyeszet

1988-06-01 Priority to DD31628488A priority Critical patent/DD270723A5/de

1989-08-09 Publication of DD270723A5 publication Critical patent/DD270723A5/de

Links

108090001069 Chymopapain Proteins 0.000 title claims abstract description 26
ISWQCIVKKSOKNN-UHFFFAOYSA-L Tiron Chemical compound [Na+].[Na+].OC1=CC(S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C1O ISWQCIVKKSOKNN-UHFFFAOYSA-L 0.000 title claims abstract description 26
229960002976 chymopapain Drugs 0.000 title claims abstract description 26
238000000034 method Methods 0.000 title claims abstract description 24
238000002360 preparation method Methods 0.000 title claims abstract description 7
108090000790 Enzymes Proteins 0.000 claims abstract description 10
102000004190 Enzymes Human genes 0.000 claims abstract description 10
229940088598 enzyme Drugs 0.000 claims abstract description 10
239000000872 buffer Substances 0.000 claims abstract description 8
239000003638 chemical reducing agent Substances 0.000 claims abstract description 8
238000005227 gel permeation chromatography Methods 0.000 claims abstract description 8
239000006228 supernatant Substances 0.000 claims abstract description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 3
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 10
235000018417 cysteine Nutrition 0.000 claims description 10
239000000243 solution Substances 0.000 claims description 9
229920005654 Sephadex Polymers 0.000 claims description 7
239000012507 Sephadex™ Substances 0.000 claims description 7
239000008363 phosphate buffer Substances 0.000 claims description 6
GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims description 6
239000008351 acetate buffer Substances 0.000 claims description 4
229910019142 PO4 Inorganic materials 0.000 claims description 3
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
235000010265 sodium sulphite Nutrition 0.000 claims description 3
XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 2
DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 claims description 2
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims description 2
238000004108 freeze drying Methods 0.000 claims 1
230000000694 effects Effects 0.000 description 18
WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 7
229910001626 barium chloride Inorganic materials 0.000 description 7
239000000126 substance Substances 0.000 description 6
239000002244 precipitate Substances 0.000 description 4
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
238000004587 chromatography analysis Methods 0.000 description 3
238000004255 ion exchange chromatography Methods 0.000 description 3
239000004816 latex Substances 0.000 description 3
229920000126 latex Polymers 0.000 description 3
241000219173 Carica Species 0.000 description 2
235000009467 Carica papaya Nutrition 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
108090000526 Papain Proteins 0.000 description 2
239000004365 Protease Substances 0.000 description 2
101710097834 Thiol protease Proteins 0.000 description 2
235000013399 edible fruits Nutrition 0.000 description 2
229940055729 papain Drugs 0.000 description 2
235000019834 papain Nutrition 0.000 description 2
238000001556 precipitation Methods 0.000 description 2
235000004252 protein component Nutrition 0.000 description 2
238000000926 separation method Methods 0.000 description 2
239000000758 substrate Substances 0.000 description 2
LHFNPUGRSYOPLF-UHFFFAOYSA-N (4-nitrophenyl) 2-(phenylmethoxycarbonylamino)acetate Chemical compound C1=CC([N+](=O)[O-])=CC=C1OC(=O)CNC(=O)OCC1=CC=CC=C1 LHFNPUGRSYOPLF-UHFFFAOYSA-N 0.000 description 1
240000006432 Carica papaya Species 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
208000003618 Intervertebral Disc Displacement Diseases 0.000 description 1
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
239000013543 active substance Substances 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
230000002052 anaphylactic effect Effects 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
208000035475 disorder Diseases 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
238000001035 drying Methods 0.000 description 1
210000002196 fr. b Anatomy 0.000 description 1
230000008014 freezing Effects 0.000 description 1
238000007710 freezing Methods 0.000 description 1
235000011389 fruit/vegetable juice Nutrition 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
230000002779 inactivation Effects 0.000 description 1
238000009413 insulation Methods 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
230000000116 mitigating effect Effects 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
230000001376 precipitating effect Effects 0.000 description 1
239000000047 product Substances 0.000 description 1
235000018102 proteins Nutrition 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
230000002797 proteolythic effect Effects 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
238000006748 scratching Methods 0.000 description 1
230000002393 scratching effect Effects 0.000 description 1

Landscapes

Enzymes And Modification Thereof (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)

DD31628488A 1986-12-08 1988-06-01 Verfahren zur Herstellung von hochreinem Chymopapain DD270723A5 (de)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
DD31628488A DD270723A5 (de)	1986-12-08	1988-06-01	Verfahren zur Herstellung von hochreinem Chymopapain

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
HU865090A HU196624B (en)	1986-12-08	1986-12-08	Process for producing chymopapain with high specific activity
DD31628488A DD270723A5 (de)	1986-12-08	1988-06-01	Verfahren zur Herstellung von hochreinem Chymopapain

Publications (1)

Publication Number	Publication Date
DD270723A5 true DD270723A5 (de)	1989-08-09

Family

ID=10969645

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD31628488A DD270723A5 (de)	1986-12-08	1988-06-01	Verfahren zur Herstellung von hochreinem Chymopapain

Country Status (5)

Country	Link
BG (1)	BG50942A3 (cs)
CS (1)	CS271346B2 (cs)
CU (1)	CU22158A3 (cs)
DD (1)	DD270723A5 (cs)
HU (1)	HU196624B (cs)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5468480A (en) *	1989-04-28	1995-11-21	The Boots Company Plc	Pharmaceutical composition of purified chymopapain
CN1320111C (zh) *	2004-08-09	2007-06-06	王美岭	一种制备木瓜凝乳酶的方法

1986
- 1986-12-08 HU HU865090A patent/HU196624B/hu not_active IP Right Cessation
1988
- 1988-05-18 CU CU8870A patent/CU22158A3/es unknown
- 1988-06-01 CS CS883773A patent/CS271346B2/cs unknown
- 1988-06-01 DD DD31628488A patent/DD270723A5/de not_active IP Right Cessation
- 1988-06-01 BG BG084338A patent/BG50942A3/bg unknown

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5468480A (en) *	1989-04-28	1995-11-21	The Boots Company Plc	Pharmaceutical composition of purified chymopapain
CN1320111C (zh) *	2004-08-09	2007-06-06	王美岭	一种制备木瓜凝乳酶的方法

Also Published As

Publication number	Publication date
CU22158A3 (es)	1994-01-31
HU196624B (en)	1988-12-28
CS271346B2 (en)	1990-09-12
CS377388A2 (en)	1989-12-13
BG50942A3 (bg)	1992-12-15

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Legal Events

Date	Code	Title	Description
1999-12-09	ENJ	Ceased due to non-payment of renewal fee