HU196624B - Process for producing chymopapain with high specific activity - Google Patents

Process for producing chymopapain with high specific activity Download PDF

Info

Publication number: HU196624B
Authority: HU; Hungary
Prior art keywords: specific activity; crude; buffer; chymopamine; chymopapain
Prior art date: 1986-12-08

Application number

HU865090A

Other languages

English (en)

Hungarian (hu)

Inventor

Laszlo Polgar

Jozsef Gaal

Sandor Virag

Laszlo Jozsa

Tibor Vizkeleti

Bence Asboth

Erzsebet Fejer

Original Assignee

Chinoin Gyogyszer Es Vegyeszet

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-12-08

Filing date

1986-12-08

Publication date

1988-12-28

1986-12-08 Application filed by Chinoin Gyogyszer Es Vegyeszet filed Critical Chinoin Gyogyszer Es Vegyeszet

1986-12-08 Priority to HU865090A priority Critical patent/HU196624B/hu

1988-05-18 Priority to CU8870A priority patent/CU22158A3/es

1988-06-01 Priority to CS883773A priority patent/CS271346B2/cs

1988-06-01 Priority to BG084338A priority patent/BG50942A3/bg

1988-06-01 Priority to DD31628488A priority patent/DD270723A5/de

1988-12-28 Publication of HU196624B publication Critical patent/HU196624B/hu

Links

238000000034 method Methods 0.000 title claims description 23
108090001069 Chymopapain Proteins 0.000 title claims description 5
ISWQCIVKKSOKNN-UHFFFAOYSA-L Tiron Chemical compound [Na+].[Na+].OC1=CC(S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C1O ISWQCIVKKSOKNN-UHFFFAOYSA-L 0.000 title claims description 5
229960002976 chymopapain Drugs 0.000 title claims description 5
230000000694 effects Effects 0.000 title description 18
108090000790 Enzymes Proteins 0.000 claims abstract description 11
102000004190 Enzymes Human genes 0.000 claims abstract description 11
239000003638 chemical reducing agent Substances 0.000 claims abstract description 8
239000000872 buffer Substances 0.000 claims abstract description 7
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 3
238000005227 gel permeation chromatography Methods 0.000 claims description 11
229940088598 enzyme Drugs 0.000 claims description 10
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 9
235000018417 cysteine Nutrition 0.000 claims description 9
229920005654 Sephadex Polymers 0.000 claims description 7
239000012507 Sephadex™ Substances 0.000 claims description 7
239000008363 phosphate buffer Substances 0.000 claims description 6
GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims description 6
229910019142 PO4 Inorganic materials 0.000 claims description 4
238000002360 preparation method Methods 0.000 claims description 4
235000010265 sodium sulphite Nutrition 0.000 claims description 3
239000008351 acetate buffer Substances 0.000 claims description 2
DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 claims description 2
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims description 2
238000004108 freeze drying Methods 0.000 claims 2
125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims 1
125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
239000006228 supernatant Substances 0.000 abstract description 2
238000004519 manufacturing process Methods 0.000 abstract 1
WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 7
229910001626 barium chloride Inorganic materials 0.000 description 7
235000009467 Carica papaya Nutrition 0.000 description 4
238000004255 ion exchange chromatography Methods 0.000 description 4
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
241000219173 Carica Species 0.000 description 3
239000004816 latex Substances 0.000 description 3
229920000126 latex Polymers 0.000 description 3
239000000463 material Substances 0.000 description 3
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
239000002244 precipitate Substances 0.000 description 3
239000000243 solution Substances 0.000 description 3
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
108090000526 Papain Proteins 0.000 description 2
239000004365 Protease Substances 0.000 description 2
230000002052 anaphylactic effect Effects 0.000 description 2
235000013399 edible fruits Nutrition 0.000 description 2
235000019834 papain Nutrition 0.000 description 2
229940055729 papain Drugs 0.000 description 2
239000010452 phosphate Substances 0.000 description 2
230000001376 precipitating effect Effects 0.000 description 2
239000011541 reaction mixture Substances 0.000 description 2
239000000126 substance Substances 0.000 description 2
LHFNPUGRSYOPLF-UHFFFAOYSA-N (4-nitrophenyl) 2-(phenylmethoxycarbonylamino)acetate Chemical compound C1=CC([N+](=O)[O-])=CC=C1OC(=O)CNC(=O)OCC1=CC=CC=C1 LHFNPUGRSYOPLF-UHFFFAOYSA-N 0.000 description 1
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
206010067484 Adverse reaction Diseases 0.000 description 1
240000006432 Carica papaya Species 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
206010019909 Hernia Diseases 0.000 description 1
XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
108091005804 Peptidases Proteins 0.000 description 1
102000035195 Peptidases Human genes 0.000 description 1
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
229910000831 Steel Inorganic materials 0.000 description 1
101710097834 Thiol protease Proteins 0.000 description 1
239000013543 active substance Substances 0.000 description 1
230000006838 adverse reaction Effects 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
239000001166 ammonium sulphate Substances 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
230000008033 biological extinction Effects 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
230000002939 deleterious effect Effects 0.000 description 1
238000001035 drying Methods 0.000 description 1
230000008014 freezing Effects 0.000 description 1
238000007710 freezing Methods 0.000 description 1
235000011389 fruit/vegetable juice Nutrition 0.000 description 1
230000002779 inactivation Effects 0.000 description 1
238000011534 incubation Methods 0.000 description 1
238000002955 isolation Methods 0.000 description 1
238000005259 measurement Methods 0.000 description 1
239000000203 mixture Substances 0.000 description 1
230000009965 odorless effect Effects 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
239000000047 product Substances 0.000 description 1
235000019833 protease Nutrition 0.000 description 1
229960000856 protein c Drugs 0.000 description 1
235000004252 protein component Nutrition 0.000 description 1
235000018102 proteins Nutrition 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
230000002797 proteolythic effect Effects 0.000 description 1
238000006748 scratching Methods 0.000 description 1
230000002393 scratching effect Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
238000007086 side reaction Methods 0.000 description 1
239000010959 steel Substances 0.000 description 1
239000000758 substrate Substances 0.000 description 1
208000024891 symptom Diseases 0.000 description 1

Landscapes

Enzymes And Modification Thereof (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)

HU865090A 1986-12-08 1986-12-08 Process for producing chymopapain with high specific activity HU196624B (en)

Priority Applications (5)

Application Number	Priority Date	Filing Date	Title
HU865090A HU196624B (en)	1986-12-08	1986-12-08	Process for producing chymopapain with high specific activity
CU8870A CU22158A3 (es)	1986-12-08	1988-05-18	Procedimiento para la fabricacion de quimopapaina de alta pureza
CS883773A CS271346B2 (en)	1986-12-08	1988-06-01	Method of high-purity chymopapain production
BG084338A BG50942A3 (bg)	1986-12-08	1988-06-01	Метод за получаване на химопапаин
DD31628488A DD270723A5 (de)	1986-12-08	1988-06-01	Verfahren zur Herstellung von hochreinem Chymopapain

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
HU865090A HU196624B (en)	1986-12-08	1986-12-08	Process for producing chymopapain with high specific activity

Publications (1)

Publication Number	Publication Date
HU196624B true HU196624B (en)	1988-12-28

Family

ID=10969645

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
HU865090A HU196624B (en)	1986-12-08	1986-12-08	Process for producing chymopapain with high specific activity

Country Status (5)

Country	Link
BG (1)	BG50942A3 (cs)
CS (1)	CS271346B2 (cs)
CU (1)	CU22158A3 (cs)
DD (1)	DD270723A5 (cs)
HU (1)	HU196624B (cs)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB8909836D0 (en) *	1989-04-28	1989-06-14	Boots Co Plc	Therapeutic agent
CN1320111C (zh) *	2004-08-09	2007-06-06	王美岭	一种制备木瓜凝乳酶的方法

1986
- 1986-12-08 HU HU865090A patent/HU196624B/hu not_active IP Right Cessation
1988
- 1988-05-18 CU CU8870A patent/CU22158A3/es unknown
- 1988-06-01 CS CS883773A patent/CS271346B2/cs unknown
- 1988-06-01 DD DD31628488A patent/DD270723A5/de not_active IP Right Cessation
- 1988-06-01 BG BG084338A patent/BG50942A3/bg unknown

Also Published As

Publication number	Publication date
CU22158A3 (es)	1994-01-31
CS271346B2 (en)	1990-09-12
DD270723A5 (de)	1989-08-09
CS377388A2 (en)	1989-12-13
BG50942A3 (bg)	1992-12-15

Legal Events

Date	Code	Title	Description
1990-06-28	HU90	Patent valid on 900628
1994-09-28	HMM4	Cancellation of final prot. due to non-payment of fee

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DK166566B1 (da)	1993-06-14	Enzympraeparat til rensningsformaal og fremstilling heraf samt anvendelse af praeparatet til ikke-terapeutisk rensning
Starcher et al.	1976	Purification and comparison of elastins from different animal species
US4439423A (en)	1984-03-27	Chymopapain and method for its use
JPS62138434A (ja)	1987-06-22	アルフア−１−プロテイナ−ゼ阻害剤の製造法
US4719108A (en)	1988-01-12	Chymopapain composition and method for its use
Taylor	1962	Proteinases of the stomach in health and disease
JP2018520707A (ja)	2018-08-02	新規なトリプシンアイソフォームおよびそれらの使用
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Yarleque et al.	1989	Isolation and characterization of a fibrinogen-clotting enzyme from venom of the snake, Lachesis muta muta (Peruvian bushmaster)
JPH0559095B2 (cs)	1993-08-30
US3019171A (en)	1962-01-30	Activated enzyme compositions
JPH0322399B2 (cs)	1991-03-26
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AU681237B2 (en)	1997-08-21	Novel protease for treating devitalized tissue
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RU2093166C1 (ru)	1997-10-20	Ранозаживляющее средство "коллагеназа кк" широкого спектра действия
US3912704A (en)	1975-10-14	Protease inhibitor from horse urine
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EP0397571A1 (fr)	1990-11-14	Utilisation du caséinoglycopeptide k pour la fabrication d'une composition, notamment d'un médicament, pour la prévention et le traitement des thromboses
CA1053661A (en)	1979-05-01	Protease-free proteins, and methods of manufacturing and using the same
JPS61162184A (ja)	1986-07-22	新規な線溶酵素およびその取得法
GB1581472A (en)	1980-12-17	Compounds of the plasminogen type processes for their production and pharmaceutical compositions containing them
JP3430176B2 (ja)	2003-07-28	アンギオテンシン変換酵素阻害ペプチドの精製方法