HU196624B - Process for producing chymopapain with high specific activity - Google Patents
Process for producing chymopapain with high specific activity Download PDFInfo
- Publication number
- HU196624B HU196624B HU865090A HU509086A HU196624B HU 196624 B HU196624 B HU 196624B HU 865090 A HU865090 A HU 865090A HU 509086 A HU509086 A HU 509086A HU 196624 B HU196624 B HU 196624B
- Authority
- HU
- Hungary
- Prior art keywords
- specific activity
- crude
- buffer
- chymopamine
- chymopapain
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 23
- 108090001069 Chymopapain Proteins 0.000 title claims description 5
- ISWQCIVKKSOKNN-UHFFFAOYSA-L Tiron Chemical compound [Na+].[Na+].OC1=CC(S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C1O ISWQCIVKKSOKNN-UHFFFAOYSA-L 0.000 title claims description 5
- 229960002976 chymopapain Drugs 0.000 title claims description 5
- 230000000694 effects Effects 0.000 title description 18
- 108090000790 Enzymes Proteins 0.000 claims abstract description 11
- 102000004190 Enzymes Human genes 0.000 claims abstract description 11
- 239000003638 chemical reducing agent Substances 0.000 claims abstract description 8
- 239000000872 buffer Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 3
- 238000005227 gel permeation chromatography Methods 0.000 claims description 11
- 229940088598 enzyme Drugs 0.000 claims description 10
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 9
- 235000018417 cysteine Nutrition 0.000 claims description 9
- 229920005654 Sephadex Polymers 0.000 claims description 7
- 239000012507 Sephadex™ Substances 0.000 claims description 7
- 239000008363 phosphate buffer Substances 0.000 claims description 6
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 235000010265 sodium sulphite Nutrition 0.000 claims description 3
- 239000008351 acetate buffer Substances 0.000 claims description 2
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 claims description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims 2
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
- 239000006228 supernatant Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
- WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 7
- 229910001626 barium chloride Inorganic materials 0.000 description 7
- 235000009467 Carica papaya Nutrition 0.000 description 4
- 238000004255 ion exchange chromatography Methods 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 241000219173 Carica Species 0.000 description 3
- 239000004816 latex Substances 0.000 description 3
- 229920000126 latex Polymers 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 108090000526 Papain Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 230000002052 anaphylactic effect Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 235000019834 papain Nutrition 0.000 description 2
- 229940055729 papain Drugs 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LHFNPUGRSYOPLF-UHFFFAOYSA-N (4-nitrophenyl) 2-(phenylmethoxycarbonylamino)acetate Chemical compound C1=CC([N+](=O)[O-])=CC=C1OC(=O)CNC(=O)OCC1=CC=CC=C1 LHFNPUGRSYOPLF-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 240000006432 Carica papaya Species 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 206010019909 Hernia Diseases 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 101710097834 Thiol protease Proteins 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 239000001166 ammonium sulphate Substances 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 229960000856 protein c Drugs 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 238000006748 scratching Methods 0.000 description 1
- 230000002393 scratching effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU865090A HU196624B (en) | 1986-12-08 | 1986-12-08 | Process for producing chymopapain with high specific activity |
| CU8870A CU22158A3 (es) | 1986-12-08 | 1988-05-18 | Procedimiento para la fabricacion de quimopapaina de alta pureza |
| BG084338A BG50942A3 (bg) | 1986-12-08 | 1988-06-01 | Метод за получаване на химопапаин |
| DD31628488A DD270723A5 (de) | 1986-12-08 | 1988-06-01 | Verfahren zur Herstellung von hochreinem Chymopapain |
| CS883773A CS271346B2 (en) | 1986-12-08 | 1988-06-01 | Method of high-purity chymopapain production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU865090A HU196624B (en) | 1986-12-08 | 1986-12-08 | Process for producing chymopapain with high specific activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HU196624B true HU196624B (en) | 1988-12-28 |
Family
ID=10969645
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HU865090A HU196624B (en) | 1986-12-08 | 1986-12-08 | Process for producing chymopapain with high specific activity |
Country Status (5)
| Country | Link |
|---|---|
| BG (1) | BG50942A3 (cs) |
| CS (1) | CS271346B2 (cs) |
| CU (1) | CU22158A3 (cs) |
| DD (1) | DD270723A5 (cs) |
| HU (1) | HU196624B (cs) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8909836D0 (en) * | 1989-04-28 | 1989-06-14 | Boots Co Plc | Therapeutic agent |
| CN1320111C (zh) * | 2004-08-09 | 2007-06-06 | 王美岭 | 一种制备木瓜凝乳酶的方法 |
-
1986
- 1986-12-08 HU HU865090A patent/HU196624B/hu not_active IP Right Cessation
-
1988
- 1988-05-18 CU CU8870A patent/CU22158A3/es unknown
- 1988-06-01 DD DD31628488A patent/DD270723A5/de not_active IP Right Cessation
- 1988-06-01 BG BG084338A patent/BG50942A3/bg unknown
- 1988-06-01 CS CS883773A patent/CS271346B2/cs unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CS377388A2 (en) | 1989-12-13 |
| CS271346B2 (en) | 1990-09-12 |
| BG50942A3 (bg) | 1992-12-15 |
| CU22158A3 (es) | 1994-01-31 |
| DD270723A5 (de) | 1989-08-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| HU90 | Patent valid on 900628 | ||
| HMM4 | Cancellation of final prot. due to non-payment of fee |