DD301620A9 - Mutierte mikrobielle alpha-amylasen mit erhoehter waerme-, saeure- und/oder alkalibestaendigkeit - Google Patents

Mutierte mikrobielle alpha-amylasen mit erhoehter waerme-, saeure- und/oder alkalibestaendigkeit Download PDF

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Publication number
DD301620A9
DD301620A9 DD90342352A DD34235290A DD301620A9 DD 301620 A9 DD301620 A9 DD 301620A9 DD 90342352 A DD90342352 A DD 90342352A DD 34235290 A DD34235290 A DD 34235290A DD 301620 A9 DD301620 A9 DD 301620A9
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German Democratic Republic
Prior art keywords
amylase
gene
mutated
mutant
enzyme
Prior art date
Application number
DD90342352A
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German (de)
English (en)
Inventor
Wilhelmus J Quax
Yves Laroche
Adrianus W H Vollebregt
Patrik Stanssens
Marc Lauwereys
Original Assignee
Gist Brocades Nv
Plant Genetic Systems Nv
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Publication date
Application filed by Gist Brocades Nv, Plant Genetic Systems Nv filed Critical Gist Brocades Nv
Priority to DD90342352A priority Critical patent/DD301620A9/de
Publication of DD301620A9 publication Critical patent/DD301620A9/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Tires In General (AREA)
  • Cigarettes, Filters, And Manufacturing Of Filters (AREA)
DD90342352A 1989-06-29 1990-06-29 Mutierte mikrobielle alpha-amylasen mit erhoehter waerme-, saeure- und/oder alkalibestaendigkeit DD301620A9 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DD90342352A DD301620A9 (de) 1989-06-29 1990-06-29 Mutierte mikrobielle alpha-amylasen mit erhoehter waerme-, saeure- und/oder alkalibestaendigkeit

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP89201735 1989-06-29
DD90342352A DD301620A9 (de) 1989-06-29 1990-06-29 Mutierte mikrobielle alpha-amylasen mit erhoehter waerme-, saeure- und/oder alkalibestaendigkeit

Publications (1)

Publication Number Publication Date
DD301620A9 true DD301620A9 (de) 1993-04-29

Family

ID=8202426

Family Applications (1)

Application Number Title Priority Date Filing Date
DD90342352A DD301620A9 (de) 1989-06-29 1990-06-29 Mutierte mikrobielle alpha-amylasen mit erhoehter waerme-, saeure- und/oder alkalibestaendigkeit

Country Status (17)

Country Link
US (1) US5364782A (ja)
EP (1) EP0410498B1 (ja)
JP (2) JP3086249B2 (ja)
KR (1) KR0165550B1 (ja)
CN (1) CN1050220A (ja)
AT (1) ATE166922T1 (ja)
BG (1) BG61081B1 (ja)
BR (1) BR9006818A (ja)
CA (1) CA2030554C (ja)
DD (1) DD301620A9 (ja)
DE (1) DE69032360T2 (ja)
DK (1) DK0410498T3 (ja)
ES (1) ES2117625T3 (ja)
FI (1) FI103285B1 (ja)
IE (1) IE902369A1 (ja)
PT (1) PT94560B (ja)
WO (1) WO1991000353A2 (ja)

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WO1991000353A3 (en) 1991-02-21
US5364782A (en) 1994-11-15
DE69032360T2 (de) 1998-12-03
WO1991000353A2 (en) 1991-01-10
AU638263B2 (en) 1993-06-24
PT94560B (pt) 1998-01-30
FI103285B (fi) 1999-05-31
AU5953890A (en) 1991-01-17
DK0410498T3 (da) 1999-03-22
IE902369A1 (en) 1991-06-19
JP3086249B2 (ja) 2000-09-11
DE69032360D1 (de) 1998-07-09
CA2030554A1 (en) 1990-12-30
PT94560A (pt) 1991-02-08
JP2000197491A (ja) 2000-07-18
CA2030554C (en) 2001-08-28
FI910907A0 (fi) 1991-02-25
BG93814A (bg) 1993-12-24
EP0410498B1 (en) 1998-06-03
KR0165550B1 (ko) 1999-01-15
IE902369L (en) 1990-12-29
KR920701449A (ko) 1992-08-11
BG61081B1 (bg) 1996-10-31
JPH04500756A (ja) 1992-02-13
EP0410498A3 (en) 1991-11-06
FI103285B1 (fi) 1999-05-31
CN1050220A (zh) 1991-03-27
EP0410498A2 (en) 1991-01-30
ATE166922T1 (de) 1998-06-15
ES2117625T3 (es) 1998-08-16
BR9006818A (pt) 1991-08-06

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