DK1002100T3 - Fremgangsmåde til fremstilling og identifikation af nye hydrolaser med forbedrede egenskaber - Google Patents

Fremgangsmåde til fremstilling og identifikation af nye hydrolaser med forbedrede egenskaber

Info

Publication number
DK1002100T3
DK1002100T3 DK98938701T DK98938701T DK1002100T3 DK 1002100 T3 DK1002100 T3 DK 1002100T3 DK 98938701 T DK98938701 T DK 98938701T DK 98938701 T DK98938701 T DK 98938701T DK 1002100 T3 DK1002100 T3 DK 1002100T3
Authority
DK
Denmark
Prior art keywords
improved properties
identification
preparation
hydrolases
novel
Prior art date
Application number
DK98938701T
Other languages
English (en)
Inventor
Manfred T Reetz
Albin Zonta
Klaus Schimossek
Klaus Liebeton
Karl-Erich Jaeger
Original Assignee
Studiengesellschaft Kohle Mbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Studiengesellschaft Kohle Mbh filed Critical Studiengesellschaft Kohle Mbh
Application granted granted Critical
Publication of DK1002100T3 publication Critical patent/DK1002100T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK98938701T 1997-07-25 1998-07-23 Fremgangsmåde til fremstilling og identifikation af nye hydrolaser med forbedrede egenskaber DK1002100T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19731990A DE19731990A1 (de) 1997-07-25 1997-07-25 Verfahren zur Herstellung und Identifizierung von neuen Hydrolasen mit verbesserten Eigenschaften
PCT/EP1998/004612 WO1999005288A2 (de) 1997-07-25 1998-07-23 Verfahren zur herstellung und identifizierung von neuen hydrolasen mit verbesserten eigenschaften

Publications (1)

Publication Number Publication Date
DK1002100T3 true DK1002100T3 (da) 2003-10-27

Family

ID=7836844

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98938701T DK1002100T3 (da) 1997-07-25 1998-07-23 Fremgangsmåde til fremstilling og identifikation af nye hydrolaser med forbedrede egenskaber

Country Status (8)

Country Link
EP (1) EP1002100B1 (da)
JP (1) JP2001512663A (da)
AT (1) ATE244309T1 (da)
CA (1) CA2298069A1 (da)
DE (2) DE19731990A1 (da)
DK (1) DK1002100T3 (da)
ES (1) ES2202883T3 (da)
WO (1) WO1999005288A2 (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19913858A1 (de) * 1999-03-26 2000-09-28 Studiengesellschaft Kohle Mbh High-Throughput-Screening-Verfahren zur Bestimmung der Enantioselektivität asymmetrisch verlaufender Reaktionen
AU4211600A (en) * 1999-04-10 2000-11-14 Maxygen, Inc. Modified lipid production
DE10042451A1 (de) * 2000-08-29 2002-03-14 Studiengesellschaft Kohle Mbh High-Throughput Verfahren zur Bestimmung der Enantiomereneinheit von chiralen Verbindungen
ES2713526T3 (es) * 2009-07-17 2019-05-22 Korea Advanced Inst Sci & Tech Procedimiento de producción de ésteres alquílicos de ácidos grasos usando microorganismos que tienen capacidad de producir aceite

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4238195A (en) * 1979-01-18 1980-12-09 Miles Laboratories, Inc. Fluorescer-labeled specific binding assays
DE3923921A1 (de) * 1989-07-19 1991-01-24 Biotechnolog Forschung Gmbh Optischer biosensor
EP0443063A1 (en) * 1990-02-22 1991-08-28 Henkel Research Corporation Pseudomonas lipase gene, vectors for the expression thereof, production of the lipase by transformed microorganisms and uses of this enzyme
CA2079802C (en) * 1990-04-05 2001-09-18 Roberto Crea Walk-through mutagenesis
DE4210970C2 (de) * 1992-04-02 1996-10-17 Markus Dipl Chem Sauer Verfahren zur simultanen optischen qualitativen und quantitativen Erfassung von verschiedenen mit Fluorochromen oder Fluorogenen markierten Molekülen eines Gemisches mittels Laserspektroskopie
JP3093039B2 (ja) * 1992-06-30 2000-10-03 長瀬産業株式会社 新規エステル分解酵素ａおよびその製造方法
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DE4408152A1 (de) * 1994-03-11 1995-09-14 Studiengesellschaft Kohle Mbh Immobilisierte Lipasen in hydrophoben Sol-Gel-Materialien
DE4413121A1 (de) * 1994-04-17 1995-02-16 Volker Prof Dr Schurig Verfahren zur Enantiomerentrennung an chiralen Stationärphasen durch Flüssigchromatographie in Kapilarsäulen
FR2722798B1 (fr) * 1994-07-25 1996-09-13 Toulouse Inst Nat Polytech 1procede de production d'acides gras2plantes oleagineuses
WO1997020918A1 (en) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Method of screening for enzyme activity

Also Published As

Publication number Publication date
ATE244309T1 (de) 2003-07-15
DE59808919D1 (de) 2003-08-07
EP1002100B1 (de) 2003-07-02
WO1999005288A3 (de) 1999-07-08
CA2298069A1 (en) 1999-02-04
DE19731990A1 (de) 1999-01-28
JP2001512663A (ja) 2001-08-28
ES2202883T3 (es) 2004-04-01
WO1999005288A2 (de) 1999-02-04
EP1002100A2 (de) 2000-05-24

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