DK1423400T3 - Evolution af hidtil ukendt molekylfunktion - Google Patents

Evolution af hidtil ukendt molekylfunktion

Info

Publication number
DK1423400T3
DK1423400T3 DK02753671T DK02753671T DK1423400T3 DK 1423400 T3 DK1423400 T3 DK 1423400T3 DK 02753671 T DK02753671 T DK 02753671T DK 02753671 T DK02753671 T DK 02753671T DK 1423400 T3 DK1423400 T3 DK 1423400T3
Authority
DK
Denmark
Prior art keywords
molecules
amplification
natural
nucleic acid
evolution
Prior art date
Application number
DK02753671T
Other languages
English (en)
Other versions
DK1423400T4 (da
Inventor
David R Liu
Zev J Gartner
Mattew W Kanan
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27493169&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1423400(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Harvard College filed Critical Harvard College
Application granted granted Critical
Publication of DK1423400T3 publication Critical patent/DK1423400T3/da
Publication of DK1423400T4 publication Critical patent/DK1423400T4/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Other Resins Obtained By Reactions Not Involving Carbon-To-Carbon Unsaturated Bonds (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Peptides Or Proteins (AREA)
  • Steroid Compounds (AREA)
DK02753671.3T 2001-03-19 2002-03-19 Evolution af hidtil ukendt molekylfunktion DK1423400T4 (da)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US27709401P 2001-03-19 2001-03-19
US27708101P 2001-03-19 2001-03-19
US30669101P 2001-07-20 2001-07-20
PCT/US2002/008546 WO2002074929A2 (en) 2001-03-19 2002-03-19 Evolving new molecular function
US10/101,030 US7070928B2 (en) 2001-03-19 2002-03-19 Evolving new molecular function

Publications (2)

Publication Number Publication Date
DK1423400T3 true DK1423400T3 (da) 2006-12-11
DK1423400T4 DK1423400T4 (da) 2013-09-02

Family

ID=27493169

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02753671.3T DK1423400T4 (da) 2001-03-19 2002-03-19 Evolution af hidtil ukendt molekylfunktion

Country Status (12)

Country Link
US (7) US7070928B2 (da)
EP (3) EP1423400B2 (da)
JP (4) JP4128453B2 (da)
AT (1) ATE335754T1 (da)
AU (2) AU2002306777C1 (da)
CA (1) CA2441820C (da)
DE (1) DE60213826T3 (da)
DK (1) DK1423400T4 (da)
ES (1) ES2271306T5 (da)
IL (4) IL158000A0 (da)
PT (1) PT1423400E (da)
WO (1) WO2002074929A2 (da)

Families Citing this family (132)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US20040121309A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
EP1423400B2 (en) * 2001-03-19 2013-05-22 President and Fellows of Harvard College Evolving new molecular function
ATE371026T1 (de) * 2001-06-20 2007-09-15 Nuevolution As Eine methode zur synthese von matrizenabhängigen molekülen
EP1401850A1 (en) 2001-06-20 2004-03-31 Nuevolution A/S Nucleoside derivatives for library preparation
US8073627B2 (en) * 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
DE10147074A1 (de) * 2001-09-25 2003-05-08 Beru Ag Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung
DE20200926U1 (de) * 2002-01-23 2002-04-18 Hegenscheidt-MFD GmbH & Co. KG, 41812 Erkelenz Festwalzgerät einer Festwalzmaschine für Kurbelwellen
CA2478203C (en) * 2002-03-08 2011-06-14 Eidgenossische Technische Hochschule Zurich Encoded self-assembling chemical libraries (esachel)
AU2003218629A1 (en) * 2002-03-15 2003-09-29 Nuevolution A/S A building block forming a c-c or a c-hetero atom bond uponreaction
EP1487978B1 (en) * 2002-03-15 2008-11-19 Nuevolution A/S An improved method for synthesising templated molecules
AU2003214033A1 (en) * 2002-03-15 2003-09-29 Nuevolution A/S A building block capable of transferring a functional entity
US20050221318A1 (en) * 2002-03-15 2005-10-06 Gouliaev Alex H Building block forming a c-c bond upon reaction
WO2004013070A2 (en) * 2002-08-01 2004-02-12 Nuevolution A/S Multi-step synthesis of templated molecules
AU2003263937B2 (en) * 2002-08-19 2010-04-01 The President And Fellows Of Harvard College Evolving new molecular function
EP1539953A2 (en) * 2002-09-12 2005-06-15 Nuevolution A/S Proximity-aided synthesis of templated molecules
US8791053B2 (en) * 2002-09-27 2014-07-29 Mpm-Holding Aps Spatially encoded polymer matrix
AU2011226815B2 (en) * 2002-10-30 2014-09-25 Nuevolution A/S Enzymatic encoding
PT2348125T (pt) 2002-10-30 2017-08-29 Nuevolution As Método para a síntese de um complexo bifuncional
JP2006516193A (ja) 2002-12-06 2006-06-29 アイシス・ファーマシューティカルス・インコーポレーテッド ヒトおよび動物における病原体の迅速な同定方法
AU2003291964A1 (en) 2002-12-19 2004-07-14 Nuevolution A/S Quasirandom structure and function guided synthesis methods
EP1597394A2 (en) * 2003-02-21 2005-11-23 Nuevolution A/S A method for obtaining structural information about an encoded molecule
US20070026397A1 (en) 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
DE602004019764D1 (de) * 2003-03-20 2009-04-16 Nuevolution As Ligationsvermittelnde codierung von kleinen molekülen
US8017323B2 (en) * 2003-03-26 2011-09-13 President And Fellows Of Harvard College Free reactant use in nucleic acid-templated synthesis
US8046171B2 (en) 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
WO2005016851A1 (en) * 2003-05-08 2005-02-24 Valent Technologies, Llc Recombined molecules and preparation thereof
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
WO2004110964A2 (en) * 2003-06-16 2004-12-23 Nuevolution A/S Encoded molecules by translation (emt)
WO2005003778A2 (en) * 2003-07-02 2005-01-13 Nuevolution A/S A method for identifying a synthetic molecule having affinity towards a target
US7956175B2 (en) 2003-09-11 2011-06-07 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
EP1670939B1 (en) 2003-09-18 2009-11-04 Nuevolution A/S A method for obtaining structural information concerning an encoded molecule and method for selecting compounds
CN1898257B (zh) 2003-12-17 2010-06-23 普雷西斯药品公司 合成编码文库的方法
US7972994B2 (en) 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
EP1723255B1 (en) 2004-02-17 2010-12-29 Nuevolution A/S Method for enrichment involving elimination by mismatch hybridisation
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US8119336B2 (en) 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
DE602005017370D1 (de) 2004-03-22 2009-12-10 Nuevolution As Ligationscodierung unter verwendung von oligonukleotidbausteinen
EP2458619B1 (en) 2004-05-24 2017-08-02 Ibis Biosciences, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
WO2005123959A2 (en) 2004-06-10 2005-12-29 Perkinelmer Las, Inc. Multiplexing assays for analyte detection
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
DK1809743T3 (da) 2004-11-08 2009-05-04 Vipergen Aps Strukturel nukleinsyre-styret kemisk syntese
EP1828381B1 (en) 2004-11-22 2009-01-07 Peter Birk Rasmussen Template directed split and mix systhesis of small molecule libraries
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
WO2006094238A2 (en) 2005-03-03 2006-09-08 Isis Pharmaceuticals, Inc. Compositions for use in identification of adventitious viruses
JP5162447B2 (ja) * 2005-04-13 2013-03-13 アイビス バイオサイエンシズ インコーポレイティッド アデノウイルスの同定に用いる組成物
JP2008541698A (ja) * 2005-05-03 2008-11-27 アンサンブル ディスカバリー コーポレイション 核酸検出のためのターンオーバープローブおよびその使用
KR20080028886A (ko) * 2005-05-26 2008-04-02 앙상블 디스커버리 코포레이션 핵산-템플레이팅된 화학 반응에 의한 생검출
US20090203530A1 (en) * 2005-06-07 2009-08-13 Liu David R Polymer evolution via templated synthesis
CA2611512C (en) 2005-06-09 2018-05-22 Praecis Pharmaceuticals, Inc. Methods for synthesis of encoded libraries
US20090035824A1 (en) * 2005-06-17 2009-02-05 Liu David R Nucleic acid-templated chemistry in organic solvents
DE602006013134D1 (de) * 2005-06-17 2010-05-06 Harvard College Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie
WO2007011722A2 (en) 2005-07-15 2007-01-25 President And Fellows Of Harvard College Reaction discovery system
CA2616281C (en) 2005-07-21 2014-04-22 Isis Pharmaceuticals, Inc. Methods for rapid identification and quantitation of mitochondrial dna variants
ATE378427T1 (de) * 2005-10-17 2007-11-15 Rina Netzwerk Rna Technologien Verfahren zur bestimmung einer unbekannten pns seqenz und seine verwendungen
EP2368868A1 (en) 2005-10-28 2011-09-28 Praecis Pharmaceuticals Inc. Methods for identifying compounds of interest using encoded libraries
HUE056836T2 (hu) 2005-12-01 2022-03-28 Nuevolution As Enzimatikus kódolási eljárások nagy könyvtárak hatékony szintéziséhez
EP2021470A1 (en) 2006-05-03 2009-02-11 Vipergen APS A method for preparing compounds by nucleic acid directed synthesis
JP5420412B2 (ja) 2006-09-14 2014-02-19 アイビス バイオサイエンシズ インコーポレイティッド 病原体の同定のための標的全ゲノム増幅方法
WO2008036273A2 (en) * 2006-09-18 2008-03-27 Ensemble Discovery Corporation Receptor family profiling
WO2008054600A2 (en) 2006-09-28 2008-05-08 Ensemble Discovery Corporation Compositions and methods for biodetection by nucleic acid-templated chemistry
JP5680304B2 (ja) 2007-02-23 2015-03-04 アイビス バイオサイエンシズ インコーポレイティッド 迅速な法医学的ｄｎａ分析法
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
CA2730565A1 (en) * 2007-07-27 2009-02-05 Ensemble Discovery Corporation Detection assays and use thereof
WO2009134470A2 (en) * 2008-01-18 2009-11-05 The Johns Hopkins University Methods for identifying eubacteria
EP2350031A2 (en) * 2008-08-20 2011-08-03 Ensemble Therapeutics Corporation Macrocyclic compounds for inhibition of tumor necrosis factor alpha
JP2012502911A (ja) * 2008-09-14 2012-02-02 インサイター インコーポレイテッド ナノスケール分子合成
WO2010033599A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
EP2344893B1 (en) 2008-09-16 2014-10-15 Ibis Biosciences, Inc. Microplate handling systems and methods
US8148163B2 (en) 2008-09-16 2012-04-03 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
EP2396803A4 (en) 2009-02-12 2016-10-26 Ibis Biosciences Inc IONIZATION PROBE ASSEMBLIES
DK2396459T3 (da) 2009-02-13 2017-08-28 X-Chem Inc Fremgangsmåde til fremstilling og screening af DNA-kodede biblioteker
DK2446033T3 (da) 2009-06-22 2014-01-27 Univ Strasbourg Fremgangsmåde til fremstilling af et addukt
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
US9194877B2 (en) 2009-07-17 2015-11-24 Ibis Biosciences, Inc. Systems for bioagent indentification
ES2628739T3 (es) 2009-10-15 2017-08-03 Ibis Biosciences, Inc. Amplificación por desplazamiento múltiple
WO2011127933A1 (en) 2010-04-16 2011-10-20 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
WO2012041633A1 (en) 2010-09-27 2012-04-05 Vipergen A method for making an enriched library
JP6040227B2 (ja) 2011-05-19 2016-12-07 アジーナ バイオサイエンス， インコーポレイテッド マルチプレックス核酸同定のための製品およびプロセス
JP6261500B2 (ja) 2011-07-22 2018-01-17 プレジデント アンド フェローズ オブ ハーバード カレッジ ヌクレアーゼ切断特異性の評価および改善
DK2748357T3 (da) 2011-09-07 2018-07-16 X Chem Inc Fremgangsmåder til mærkning af DNA-kodede biblioteker
EA201992285A1 (ru) 2012-07-13 2020-05-31 Икс-Чем, Инк. Днк-кодированные библиотеки, содержащие связи кодирующих олигонуклеотидов, не доступные для считывания полимеразами
US10983118B2 (en) * 2013-03-15 2021-04-20 Arizona Board Of Regents On Behalf Of Arizona State University Biosensor microarray compositions and methods
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
GB201322692D0 (en) 2013-12-20 2014-02-05 Philochem Ag Production of encoded chemical libraries
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US10040048B1 (en) 2014-09-25 2018-08-07 Synthego Corporation Automated modular system and method for production of biopolymers
EP3218479A1 (en) 2014-11-11 2017-09-20 Nanocore ApS Method for identification of molecules with desired characteristics
EP4372091A3 (en) 2014-12-12 2024-07-31 Tod M. Woolf Compositions and methods for editing nucleic acids in cells utilizing oligonucleotides
WO2016172571A1 (en) 2015-04-24 2016-10-27 Agena Bioscience, Inc. Multiplexed method for the identification and quantitation of minor alleles and polymorphisms
JP7109784B2 (ja) 2015-10-23 2022-08-01 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子編集のための進化したＣａｓ９蛋白質
US11186836B2 (en) 2016-06-16 2021-11-30 Haystack Sciences Corporation Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules
KR102547316B1 (ko) 2016-08-03 2023-06-23 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
WO2018081534A1 (en) 2016-10-28 2018-05-03 President And Fellows Of Harvard College Assay for exo-site binding molecules
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510038A (ja) 2017-03-09 2020-04-02 プレジデント アンド フェローズ オブ ハーバード カレッジ がんワクチン
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
EP3619340A4 (en) 2017-05-02 2021-01-20 Haystack Sciences Corporation OLIGONUCLEOTID-DRIVEN COMBINATORY SYNTHESIS VERIFICATION MOLECULES AND ASSOCIATED MANUFACTURING AND USE PROCESSES
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
US20190076814A1 (en) 2017-09-11 2019-03-14 Synthego Corporation Biopolymer synthesis system and method
EP3681637A4 (en) * 2017-09-11 2021-06-02 Synthego Corporation SYSTEM AND PROCEDURE FOR BIOPOLYMER SYNTHESIS
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US11674136B2 (en) 2018-02-09 2023-06-13 President And Fellows Of Harvard College DNA-templated macrocycle library
EP3797160A1 (en) 2018-05-23 2021-03-31 The Broad Institute Inc. Base editors and uses thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
WO2020124213A1 (en) 2018-12-20 2020-06-25 Krylov Sergey N Binder selection using capillary electrophoresis
EP3914704B9 (en) 2019-01-22 2023-10-04 Vipergen APS A method for screening of an in vitro display library within a cell
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
JP7657726B2 (ja) 2019-03-19 2025-04-07 ザ ブロード インスティテュート，インコーポレーテッド 編集ヌクレオチド配列を編集するための方法および組成物
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz
CN116676674A (zh) * 2022-02-22 2023-09-01 成都先导药物开发股份有限公司 一种dna编码化合物库中的c-c键偶联的方法
US20250177558A1 (en) * 2022-02-28 2025-06-05 The Board Of Trustees Of The Leland Stanford Junior University Functionalized Nanoclusters and Their Use in Treating Bacterial Infections

Family Cites Families (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US277081A (en) 1883-05-08 Scalding napped hats and sizing or felting hat and other fabrics
US4194550A (en) * 1976-01-26 1980-03-25 Insulating Shade (Limited Partnership) Apparatus for insulating against conductive, convective and radiant heat transmission
US4863857A (en) 1985-03-01 1989-09-05 Board Of Regents, The University Of Texas System Polypeptide complementary to peptides or proteins having an amino acid sequence or nucleotide coding sequence at least partially known
US5506337A (en) 1985-03-15 1996-04-09 Antivirals Inc. Morpholino-subunit combinatorial library and method
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4703034A (en) * 1986-04-28 1987-10-27 Merck & Co., Inc. Novel cyclic tetrapeptide
US5449602A (en) * 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5162218A (en) 1988-11-18 1992-11-10 The Regents Of The University Of California Conjugated polypeptides and methods for their preparation
US6680192B1 (en) 1989-05-16 2004-01-20 Scripps Research Institute Method for producing polymers having a preselected activity
US6291160B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
US6291161B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertiore
DK0494955T3 (da) 1989-10-05 1998-10-26 Optein Inc Cellefri syntese og isolering af hidtil ukendte gener og polypeptider
US5637682A (en) 1990-06-11 1997-06-10 Nexstar Pharmaceuticals, Inc. High-affinity oligonucleotide ligands to the tachykinin substance P
US5660985A (en) 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5723289A (en) 1990-06-11 1998-03-03 Nexstar Pharmaceuticals, Inc. Parallel selex
AU8498091A (en) 1990-08-02 1992-03-02 Regents Of The University Of Colorado, The Systematic polypeptide evolution by reverse translation
US5843701A (en) 1990-08-02 1998-12-01 Nexstar Pharmaceticals, Inc. Systematic polypeptide evolution by reverse translation
US20020038000A1 (en) 1990-08-02 2002-03-28 Larry Gold Systematic polypeptide evolution by reverse translation
US6037120A (en) 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
US6140496A (en) 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5719262A (en) 1993-11-22 1998-02-17 Buchardt, Deceased; Ole Peptide nucleic acids having amino acid side chains
US5641625A (en) 1992-05-22 1997-06-24 Isis Pharmaceuticals, Inc. Cleaving double-stranded DNA with peptide nucleic acids
DE59209203D1 (de) 1991-08-28 1998-03-26 Boehringer Mannheim Gmbh Primer für matrizenabhängige enzymatische nukleinsäuresynthesen
JP2001524926A (ja) 1991-09-18 2001-12-04 アフィマックス テクノロジーズ ナームロゼ フェンノートシャップ オリゴマーの雑多ライブラリーの集合体を合成する方法
US5639603A (en) 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
DE69233501T2 (de) 1991-11-22 2006-02-23 Affymetrix, Inc. (n.d.Ges.d.Staates Delaware), Santa Clara Kombinatorische Strategien für die Polymersynthese
US5573905A (en) * 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5565324A (en) * 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5721099A (en) 1992-10-01 1998-02-24 Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US6436635B1 (en) 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
CA2153387A1 (en) 1993-01-07 1994-07-21 Hubert Koester Dna sequencing by mass spectrometry
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
ATE220114T1 (de) 1993-03-19 2002-07-15 Sequenom Inc Dns-sequenzbestimmung durch massenspektrometrie auf dem weg des abbaus mit exonuklease
US6074823A (en) 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US5840485A (en) 1993-05-27 1998-11-24 Selectide Corporation Topologically segregated, encoded solid phase libraries
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5922545A (en) 1993-10-29 1999-07-13 Affymax Technologies N.V. In vitro peptide and antibody display libraries
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
WO1995014106A2 (en) 1993-11-17 1995-05-26 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
US6127154A (en) 1994-02-10 2000-10-03 Mosbach; Klaus Methods for direct synthesis of compounds having complementary structure to a desired molecular entity and use thereof
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US20030099945A1 (en) 1994-09-20 2003-05-29 Invenux, Inc. Parallel selex
US6048698A (en) 1994-09-20 2000-04-11 Nexstar Pharmaceuticals, Inc. Parallel SELEX™
US5597697A (en) 1994-09-30 1997-01-28 Diamond; Paul Screening assay for inhibitors and activators of RNA and DNA-dependent nucleic acid polymerases
GB9524630D0 (en) * 1994-12-24 1996-01-31 Zeneca Ltd Chemical compounds
US5559000A (en) 1995-01-18 1996-09-24 The Scripps Research Institute Encoded reaction cassette
DE19646372C1 (de) 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Genotyp und Phänotyp koppelnde Verbindung
US5846839A (en) 1995-12-22 1998-12-08 Glaxo Group Limited Methods for hard-tagging an encoded synthetic library
US5888971A (en) * 1996-02-20 1999-03-30 Ortho Pharmaceutical Corporation, Inc. Macrocyclic peptides useful in the treatment of thrombin related disorders
EP0892808B1 (en) 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
US5998140A (en) 1996-07-31 1999-12-07 The Scripps Research Institute Complex formation between dsDNA and oligomer of cyclic heterocycles
US6140053A (en) 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US5958703A (en) 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
US6080826A (en) 1997-01-06 2000-06-27 California Institute Of Technology Template-directed ring-closing metathesis and ring-opening metathesis polymerization of functionalized dienes
WO1998030575A1 (en) * 1997-01-08 1998-07-16 Proligo Llc Bioconjugation of macromolecules
US6261804B1 (en) 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
DK1712623T3 (da) 1997-01-21 2012-02-06 Gen Hospital Corp Udvælgelse af proteiner ved anvendelse af RNA-proteinfusioner
US6969584B2 (en) 1997-06-12 2005-11-29 Rigel Pharmaceuticals, Inc. Combinatorial enzymatic complexes
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
WO1999037806A2 (en) 1998-01-27 1999-07-29 Cytocell Limited Modified nucleic acid probes and uses thereof
US5945325A (en) 1998-04-20 1999-08-31 California Institute Of Technology Thermally stable para-nitrobenzyl esterases
US6287765B1 (en) 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6175001B1 (en) 1998-10-16 2001-01-16 The Scripps Research Institute Functionalized pyrimidine nucleosides and nucleotides and DNA's incorporating same
EP1123305A1 (en) * 1998-10-19 2001-08-16 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
DE69935248T2 (de) 1998-12-02 2007-11-08 Adnexus Therapeutics, Inc., Waltham Dna-protein fusionen sowie anwendungen derselben
US6831071B2 (en) 1999-04-08 2004-12-14 Pavel Sergeev Synthesis of biologically active compounds in cells
AU7057500A (en) * 1999-08-12 2001-03-13 Maxygen, Inc. Evolution and use of enzymes for combinatorial and medicinal chemistry
AU779653B2 (en) * 1999-08-27 2005-02-03 Bristol-Myers Squibb Company Methods for encoding and sorting in vitro translated proteins
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
EP1423400B2 (en) 2001-03-19 2013-05-22 President and Fellows of Harvard College Evolving new molecular function
EP1401850A1 (en) 2001-06-20 2004-03-31 Nuevolution A/S Nucleoside derivatives for library preparation
US20030143561A1 (en) 2001-06-20 2003-07-31 Nuevolution A/S Nucleoside derivatives for library preparation
ATE371026T1 (de) 2001-06-20 2007-09-15 Nuevolution As Eine methode zur synthese von matrizenabhängigen molekülen
US20030148335A1 (en) 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
CA2478203C (en) 2002-03-08 2011-06-14 Eidgenossische Technische Hochschule Zurich Encoded self-assembling chemical libraries (esachel)
US20050221318A1 (en) 2002-03-15 2005-10-06 Gouliaev Alex H Building block forming a c-c bond upon reaction
AU2003214033A1 (en) 2002-03-15 2003-09-29 Nuevolution A/S A building block capable of transferring a functional entity
EP1487848A2 (en) 2002-03-15 2004-12-22 Nuevolution A/S A building block forming a c=c double bond upon reaction
EP1487978B1 (en) 2002-03-15 2008-11-19 Nuevolution A/S An improved method for synthesising templated molecules
AU2003218629A1 (en) 2002-03-15 2003-09-29 Nuevolution A/S A building block forming a c-c or a c-hetero atom bond uponreaction
WO2003082901A2 (en) 2002-03-22 2003-10-09 Emory University Template-driven processes for synthesizing polymers and components related to such processes
EP1520040A2 (en) 2002-06-20 2005-04-06 Nuevolution A/S Microarrays displaying encoded molecules
WO2004013070A2 (en) 2002-08-01 2004-02-12 Nuevolution A/S Multi-step synthesis of templated molecules
AU2003263937B2 (en) 2002-08-19 2010-04-01 The President And Fellows Of Harvard College Evolving new molecular function
EP1539953A2 (en) 2002-09-12 2005-06-15 Nuevolution A/S Proximity-aided synthesis of templated molecules
PT2348125T (pt) 2002-10-30 2017-08-29 Nuevolution As Método para a síntese de um complexo bifuncional
AU2003291964A1 (en) 2002-12-19 2004-07-14 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US20070026397A1 (en) 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
EP1597394A2 (en) 2003-02-21 2005-11-23 Nuevolution A/S A method for obtaining structural information about an encoded molecule
DE602004019764D1 (de) 2003-03-20 2009-04-16 Nuevolution As Ligationsvermittelnde codierung von kleinen molekülen
US8017323B2 (en) 2003-03-26 2011-09-13 President And Fellows Of Harvard College Free reactant use in nucleic acid-templated synthesis
WO2004110964A2 (en) 2003-06-16 2004-12-23 Nuevolution A/S Encoded molecules by translation (emt)
WO2005003778A2 (en) 2003-07-02 2005-01-13 Nuevolution A/S A method for identifying a synthetic molecule having affinity towards a target
ATE461279T1 (de) * 2005-06-07 2010-04-15 Harvard College Geordnete mehrschrittsynthese mittels nukleinsäurevermittelter chemie
DE602006013134D1 (de) 2005-06-17 2010-05-06 Harvard College Iterierte verzweigende reaktionswege über nukleinsäure-vermittelte chemie
US20090035824A1 (en) * 2005-06-17 2009-02-05 Liu David R Nucleic acid-templated chemistry in organic solvents

Also Published As

Publication number Publication date
JP2004536162A (ja) 2004-12-02
US20050233381A1 (en) 2005-10-20
DE60213826T3 (de) 2013-10-17
DE60213826T8 (de) 2007-08-30
WO2002074929A3 (en) 2003-05-30
IL206586A (en) 2012-12-31
JP2008183010A (ja) 2008-08-14
US20050025766A1 (en) 2005-02-03
EP1423400A4 (en) 2004-12-01
EP1832567A3 (en) 2009-08-26
ATE335754T1 (de) 2006-09-15
AU2002306777B2 (en) 2007-10-04
DK1423400T4 (da) 2013-09-02
JP2009232869A (ja) 2009-10-15
CA2441820A1 (en) 2002-09-26
EP2332896A2 (en) 2011-06-15
JP5509270B2 (ja) 2014-06-04
US20030113738A1 (en) 2003-06-19
DE60213826D1 (de) 2006-09-21
EP1423400B2 (en) 2013-05-22
EP1423400A2 (en) 2004-06-02
JP2013010761A (ja) 2013-01-17
JP4384229B2 (ja) 2009-12-16
US20050227281A1 (en) 2005-10-13
US7998904B2 (en) 2011-08-16
US7070928B2 (en) 2006-07-04
WO2002074929A2 (en) 2002-09-26
PT1423400E (pt) 2006-12-29
US7557068B2 (en) 2009-07-07
JP4128453B2 (ja) 2008-07-30
EP1832567A2 (en) 2007-09-12
AU2008200027A1 (en) 2008-01-31
US20120165228A1 (en) 2012-06-28
IL206586A0 (en) 2010-12-30
IL158000A (en) 2014-08-31
IL158000A0 (en) 2004-03-28
AU2002306777C1 (en) 2008-04-24
ES2271306T5 (es) 2013-10-16
IL206585A (en) 2015-04-30
ES2271306T3 (es) 2007-04-16
US20050142583A1 (en) 2005-06-30
AU2008200027B2 (en) 2010-05-27
US20050042669A1 (en) 2005-02-24
US20050281819A9 (en) 2005-12-22
DE60213826T2 (de) 2007-03-29
EP2332896A3 (en) 2012-09-26
IL206585A0 (en) 2010-12-30
US7442160B2 (en) 2008-10-28
US7223545B2 (en) 2007-05-29
CA2441820C (en) 2014-01-07
EP1423400B1 (en) 2006-08-09

Similar Documents

Publication Publication Date Title
DK1423400T4 (da) Evolution af hidtil ukendt molekylfunktion
WO2004016767A3 (en) Evolving new molecular function
ATE312942T1 (de) Amplifizierung unter verwendung von modifizierten primern
DK0988378T4 (da) En fremgangsmåde til in vitro molekylær udvikling af proteinfunktion
ATE432365T1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
DE602004012299D1 (de) Verfahren zur verknüpfung interessierender sequenzen
HUP0100037A2 (hu) Genomiális eljárás segítségével azonosított fehérjék funkió szerinti osztályozására szolgáló nagy teljesítményű eljárás
DE60226182D1 (de) Agarase und Gen dafür
WO2018064908A1 (zh) Dna编码分子库的合成方法及dna模板
DK1504098T4 (da) Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion
DK1947104T3 (da) Fremgangsmåder og sammensætninger til identifikation og karakterisering af hepatitis C
DK1442134T3 (da) Fremgangsmåde til fremstilling og identifikation af oplöselige proteindomæner
DK1948794T3 (da) Fremgangsmåde til molekylær evolution in vitro af proteinfunktion
DK1156107T3 (da) Fremgangsmåde til at analysere genekspressionsfrekvens
ATE317123T1 (de) Reaktiver träger zur bestimmung von dna fragmenten
DE60229966D1 (de) Diabetesmodell
JP2007267733A (ja) 機能性非天然型タンパク質の製造法、及び当該タンパク質の部位特異的修飾・固定化法
DE69841783D1 (de) Verfahren zur Bestimmung des Histocompatibiltätsantigenlokus Klasse II
ATE486932T1 (de) Thermostabile dna polymerase von archae ampullavirus abv und dessen verwendungen
CY1117515T1 (el) Mια βελτιωμενη μεθοδος για τη συνθεση σχηματιζομενων απο εκμαγειο μοριων
CY1113998T1 (el) Πρωτεϊνες που δεσμευουν αλβουμινη ορου
SE0101700D0 (sv) Nucleotide sequences
DE60228947D1 (de) Verfahren zur bestimmung sekundärer molekülmodifikationen unter verwendung von arrays