DK176140B1 - Flagermusspyt-plasminogenaktivatorer - Google Patents

Flagermusspyt-plasminogenaktivatorer Download PDF

Info

Publication number: DK176140B1
Authority: DK; Denmark
Prior art keywords: gly; ser; leu; thr; cys
Prior art date: 1988-07-20

Application number

DK198903575A

Other languages

Danish (da)

English (en)

Other versions

DK357589A (da

DK357589D0 (da

Inventor

Paul A Friedman

George E Mark

Le Thi Duong

Stephen J Gardell

John W Jacobs

Richard A F Dixon

Bruce L Daugherty

Original Assignee

Schering Ag Patentabteilung

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-07-20

Filing date

1989-07-19

Publication date

2006-09-25

1989-07-19 Application filed by Schering Ag Patentabteilung filed Critical Schering Ag Patentabteilung

1989-07-19 Publication of DK357589D0 publication Critical patent/DK357589D0/da

1990-01-23 Publication of DK357589A publication Critical patent/DK357589A/da

2006-09-25 Application granted granted Critical

2006-09-25 Publication of DK176140B1 publication Critical patent/DK176140B1/da

Links

102000001938 Plasminogen Activators Human genes 0.000 title claims abstract description 86
108010001014 Plasminogen Activators Proteins 0.000 title claims abstract description 84
229940127126 plasminogen activator Drugs 0.000 title claims abstract description 82
BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 claims abstract description 82
229950003499 fibrin Drugs 0.000 claims abstract description 82
108010073385 Fibrin Proteins 0.000 claims abstract description 81
102000009123 Fibrin Human genes 0.000 claims abstract description 81
108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 17
108090000288 Glycoproteins Proteins 0.000 claims abstract description 9
102000003886 Glycoproteins Human genes 0.000 claims abstract description 9
108090000623 proteins and genes Proteins 0.000 claims description 137
102000004169 proteins and genes Human genes 0.000 claims description 113
102000013566 Plasminogen Human genes 0.000 claims description 67
108010051456 Plasminogen Proteins 0.000 claims description 67
150000001413 amino acids Chemical group 0.000 claims description 39
210000004027 cell Anatomy 0.000 claims description 39
241000288673 Chiroptera Species 0.000 claims description 38
230000003213 activating effect Effects 0.000 claims description 31
239000013612 plasmid Substances 0.000 claims description 20
238000000034 method Methods 0.000 claims description 19
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims description 16
238000010367 cloning Methods 0.000 claims description 15
230000001580 bacterial effect Effects 0.000 claims description 13
241000288900 Desmodus rotundus Species 0.000 claims description 12
229940080469 phosphocellulose Drugs 0.000 claims description 12
108010050848 glycylleucine Proteins 0.000 claims description 11
238000004128 high performance liquid chromatography Methods 0.000 claims description 10
108010002311 N-glycylglutamic acid Proteins 0.000 claims description 9
108010069495 cysteinyltyrosine Proteins 0.000 claims description 9
239000012134 supernatant fraction Substances 0.000 claims description 9
LGQPPBQRUBVTIF-JBDRJPRFSA-N Ala-Ala-Ile Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O LGQPPBQRUBVTIF-JBDRJPRFSA-N 0.000 claims description 8
QKHWNPQNOHEFST-VZFHVOOUSA-N Ala-Thr-Cys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](C)N)O QKHWNPQNOHEFST-VZFHVOOUSA-N 0.000 claims description 8
HAYVTMHUNMMXCV-IMJSIDKUSA-N Cys-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H](N)CS HAYVTMHUNMMXCV-IMJSIDKUSA-N 0.000 claims description 8
LKUCSUGWHYVYLP-GHCJXIJMSA-N Cys-Ile-Asn Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CS)N LKUCSUGWHYVYLP-GHCJXIJMSA-N 0.000 claims description 8
108010043984 Erythrina caffra trypsin inhibitor Proteins 0.000 claims description 8
AXVIGSRGTMNSJU-YESZJQIVSA-N Leu-Tyr-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N2CCC[C@@H]2C(=O)O)N AXVIGSRGTMNSJU-YESZJQIVSA-N 0.000 claims description 8
KQNDIKOYWZTZIX-FXQIFTODSA-N Ser-Ser-Arg Chemical compound OC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCCNC(N)=N KQNDIKOYWZTZIX-FXQIFTODSA-N 0.000 claims description 8
108010016616 cysteinylglycine Proteins 0.000 claims description 8
108010066198 glycyl-leucyl-phenylalanine Proteins 0.000 claims description 8
NXSFUECZFORGOG-CIUDSAMLSA-N Ala-Asn-Leu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(O)=O NXSFUECZFORGOG-CIUDSAMLSA-N 0.000 claims description 7
FOHXUHGZZKETFI-JBDRJPRFSA-N Ala-Ile-Cys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](C)N FOHXUHGZZKETFI-JBDRJPRFSA-N 0.000 claims description 7
OGZBJJLRKQZRHL-KJEVXHAQSA-N Arg-Thr-Tyr Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 OGZBJJLRKQZRHL-KJEVXHAQSA-N 0.000 claims description 7
PIWWUBYJNONVTJ-ZLUOBGJFSA-N Asn-Asp-Asn Chemical compound C([C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)N)C(=O)O)N)C(=O)N PIWWUBYJNONVTJ-ZLUOBGJFSA-N 0.000 claims description 7
KABHAOSDMIYXTR-GUBZILKMSA-N Cys-Glu-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CS)N KABHAOSDMIYXTR-GUBZILKMSA-N 0.000 claims description 7
PDRMRVHPAQKTLT-NAKRPEOUSA-N Cys-Ile-Val Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C(C)C)C(O)=O PDRMRVHPAQKTLT-NAKRPEOUSA-N 0.000 claims description 7
WTXCNOPZMQRTNN-BWBBJGPYSA-N Cys-Thr-Ser Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CS)N)O WTXCNOPZMQRTNN-BWBBJGPYSA-N 0.000 claims description 7
MRWYPDWDZSLWJM-ACZMJKKPSA-N Glu-Ser-Asp Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O MRWYPDWDZSLWJM-ACZMJKKPSA-N 0.000 claims description 7
ABPRMMYHROQBLY-NKWVEPMBSA-N Gly-Ser-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CO)NC(=O)CN)C(=O)O ABPRMMYHROQBLY-NKWVEPMBSA-N 0.000 claims description 7
TXLQHACKRLWYCM-DCAQKATOSA-N His-Glu-Glu Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O TXLQHACKRLWYCM-DCAQKATOSA-N 0.000 claims description 7
OYQUOLRTJHWVSQ-SRVKXCTJSA-N Leu-His-Ser Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(O)=O OYQUOLRTJHWVSQ-SRVKXCTJSA-N 0.000 claims description 7
HVHRPWQEQHIQJF-AVGNSLFASA-N Leu-Lys-Glu Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(O)=O HVHRPWQEQHIQJF-AVGNSLFASA-N 0.000 claims description 7
VCHVSKNMTXWIIP-SRVKXCTJSA-N Leu-Lys-Ser Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O VCHVSKNMTXWIIP-SRVKXCTJSA-N 0.000 claims description 7
BMVFXOQHDQZAQU-DCAQKATOSA-N Leu-Pro-Asp Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)O)C(=O)O)N BMVFXOQHDQZAQU-DCAQKATOSA-N 0.000 claims description 7
VULJUQZPSOASBZ-SRVKXCTJSA-N Leu-Pro-Glu Chemical compound [H]N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(O)=O VULJUQZPSOASBZ-SRVKXCTJSA-N 0.000 claims description 7
SITLTJHOQZFJGG-UHFFFAOYSA-N N-L-alpha-glutamyl-L-valine Natural products CC(C)C(C(O)=O)NC(=O)C(N)CCC(O)=O SITLTJHOQZFJGG-UHFFFAOYSA-N 0.000 claims description 7
KAHUBGWSIQNZQQ-KKUMJFAQSA-N Phe-Asn-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](N)CC1=CC=CC=C1 KAHUBGWSIQNZQQ-KKUMJFAQSA-N 0.000 claims description 7
ZENDEDYRYVHBEG-SRVKXCTJSA-N Phe-Asp-Asp Chemical compound OC(=O)C[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CC1=CC=CC=C1 ZENDEDYRYVHBEG-SRVKXCTJSA-N 0.000 claims description 7
102000012335 Plasminogen Activator Inhibitor 1 Human genes 0.000 claims description 7
108010022233 Plasminogen Activator Inhibitor 1 Proteins 0.000 claims description 7
OQPNSDWGAMFJNU-QWRGUYRKSA-N Ser-Gly-Tyr Chemical compound OC[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 OQPNSDWGAMFJNU-QWRGUYRKSA-N 0.000 claims description 7
XPNSAQMEAVSQRD-FBCQKBJTSA-N Thr-Gly-Gly Chemical compound C[C@@H](O)[C@H](N)C(=O)NCC(=O)NCC(O)=O XPNSAQMEAVSQRD-FBCQKBJTSA-N 0.000 claims description 7
NZRUWPIYECBYRK-HTUGSXCWSA-N Thr-Phe-Glu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(O)=O)C(O)=O NZRUWPIYECBYRK-HTUGSXCWSA-N 0.000 claims description 7
102000003978 Tissue Plasminogen Activator Human genes 0.000 claims description 7
108090000373 Tissue Plasminogen Activator Proteins 0.000 claims description 7
UKINEYBQXPMOJO-UBHSHLNASA-N Trp-Asn-Ser Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CO)C(=O)O)N UKINEYBQXPMOJO-UBHSHLNASA-N 0.000 claims description 7
DTPWXZXGFAHEKL-NWLDYVSISA-N Trp-Thr-Glu Chemical compound [H]N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(O)=O DTPWXZXGFAHEKL-NWLDYVSISA-N 0.000 claims description 7
ZXAGTABZUOMUDO-GVXVVHGQSA-N Val-Glu-Lys Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)O)N ZXAGTABZUOMUDO-GVXVVHGQSA-N 0.000 claims description 7
QSPOLEBZTMESFY-SRVKXCTJSA-N Val-Pro-Val Chemical compound CC(C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(O)=O QSPOLEBZTMESFY-SRVKXCTJSA-N 0.000 claims description 7
108010045126 glycyl-tyrosyl-glycine Proteins 0.000 claims description 7
108010024607 phenylalanylalanine Proteins 0.000 claims description 7
108010072986 threonyl-seryl-lysine Proteins 0.000 claims description 7
ZXJZGWOMAFPSJH-DCAQKATOSA-N (2S)-1-[2-[[2-[[(2S)-2-[[(2S)-2-[(2-aminoacetyl)amino]-3-carboxypropanoyl]amino]-3-hydroxypropanoyl]amino]acetyl]amino]acetyl]pyrrolidine-2-carboxylic acid Chemical compound [H]NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)NCC(=O)NCC(=O)N1CCC[C@H]1C(O)=O ZXJZGWOMAFPSJH-DCAQKATOSA-N 0.000 claims description 6
ANGAOPNEPIDLPO-XVYDVKMFSA-N Ala-His-Cys Chemical compound C[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)N[C@@H](CS)C(=O)O)N ANGAOPNEPIDLPO-XVYDVKMFSA-N 0.000 claims description 6
PGNNQOJOEGFAOR-KWQFWETISA-N Ala-Tyr-Gly Chemical compound OC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](N)C)CC1=CC=C(O)C=C1 PGNNQOJOEGFAOR-KWQFWETISA-N 0.000 claims description 6
GMRGSBAMMMVDGG-GUBZILKMSA-N Asn-Arg-Arg Chemical compound C(C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)NC(=O)[C@H](CC(=O)N)N)CN=C(N)N GMRGSBAMMMVDGG-GUBZILKMSA-N 0.000 claims description 6
HMWBPUDETPKSSS-DCAQKATOSA-N Cys-Pro-Lys Chemical compound C1C[C@H](N(C1)C(=O)[C@H](CS)N)C(=O)N[C@@H](CCCCN)C(=O)O HMWBPUDETPKSSS-DCAQKATOSA-N 0.000 claims description 6
ALOBJFDJTMQQPW-ONGXEEELSA-N Gly-His-Val Chemical compound CC(C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CN=CN1)NC(=O)CN ALOBJFDJTMQQPW-ONGXEEELSA-N 0.000 claims description 6
IUKIDFVOUHZRAK-QWRGUYRKSA-N Gly-Lys-His Chemical compound NCCCC[C@H](NC(=O)CN)C(=O)N[C@H](C(O)=O)CC1=CN=CN1 IUKIDFVOUHZRAK-QWRGUYRKSA-N 0.000 claims description 6
AFMOTCMSEBITOE-YEPSODPASA-N Gly-Val-Thr Chemical compound NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O AFMOTCMSEBITOE-YEPSODPASA-N 0.000 claims description 6
LNMKRJJLEFASGA-BZSNNMDCSA-N Lys-Phe-Leu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(C)C)C(O)=O LNMKRJJLEFASGA-BZSNNMDCSA-N 0.000 claims description 6
XZFYRXDAULDNFX-UHFFFAOYSA-N N-L-cysteinyl-L-phenylalanine Natural products SCC(N)C(=O)NC(C(O)=O)CC1=CC=CC=C1 XZFYRXDAULDNFX-UHFFFAOYSA-N 0.000 claims description 6
108010047562 NGR peptide Proteins 0.000 claims description 6
CJZTUKSFZUSNCC-FXQIFTODSA-N Pro-Asp-Asn Chemical compound NC(=O)C[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H]1CCCN1 CJZTUKSFZUSNCC-FXQIFTODSA-N 0.000 claims description 6
IAOHCSQDQDWRQU-GUBZILKMSA-N Ser-Val-Arg Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O IAOHCSQDQDWRQU-GUBZILKMSA-N 0.000 claims description 6
KZTLZZQTJMCGIP-ZJDVBMNYSA-N Thr-Val-Thr Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O KZTLZZQTJMCGIP-ZJDVBMNYSA-N 0.000 claims description 6
KOSRFJWDECSPRO-UHFFFAOYSA-N alpha-L-glutamyl-L-glutamic acid Natural products OC(=O)CCC(N)C(=O)NC(CCC(O)=O)C(O)=O KOSRFJWDECSPRO-UHFFFAOYSA-N 0.000 claims description 6
108010060035 arginylproline Proteins 0.000 claims description 6
108020001507 fusion proteins Proteins 0.000 claims description 6
102000037865 fusion proteins Human genes 0.000 claims description 6
108010049041 glutamylalanine Proteins 0.000 claims description 6
108010089804 glycyl-threonine Proteins 0.000 claims description 6
108010040030 histidinoalanine Proteins 0.000 claims description 6
108010053037 kyotorphin Proteins 0.000 claims description 6
108010003700 lysyl aspartic acid Proteins 0.000 claims description 6
108010064235 lysylglycine Proteins 0.000 claims description 6
108010029020 prolylglycine Proteins 0.000 claims description 6
YYSWCHMLFJLLBJ-ZLUOBGJFSA-N Ala-Ala-Ser Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O YYSWCHMLFJLLBJ-ZLUOBGJFSA-N 0.000 claims description 5
MMLHRUJLOUSRJX-CIUDSAMLSA-N Ala-Ser-Lys Chemical compound C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCCCN MMLHRUJLOUSRJX-CIUDSAMLSA-N 0.000 claims description 5
UISQLSIBJKEJSS-GUBZILKMSA-N Arg-Arg-Ser Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CO)C(O)=O UISQLSIBJKEJSS-GUBZILKMSA-N 0.000 claims description 5
WOZDCBHUGJVJPL-AVGNSLFASA-N Arg-Val-Lys Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N WOZDCBHUGJVJPL-AVGNSLFASA-N 0.000 claims description 5
SPKCGKRUYKMDHP-GUDRVLHUSA-N Asp-Ile-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CC(=O)O)N SPKCGKRUYKMDHP-GUDRVLHUSA-N 0.000 claims description 5
JDDYEZGPYBBPBN-JRQIVUDYSA-N Asp-Thr-Tyr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O JDDYEZGPYBBPBN-JRQIVUDYSA-N 0.000 claims description 5
PRVVCRZLTJNPCS-FXQIFTODSA-N Cys-Arg-Asn Chemical compound C(C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CS)N)CN=C(N)N PRVVCRZLTJNPCS-FXQIFTODSA-N 0.000 claims description 5
QUQHPUMRFGFINP-BPUTZDHNSA-N Cys-Trp-Val Chemical compound CC(C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CNC2=CC=CC=C21)NC(=O)[C@H](CS)N QUQHPUMRFGFINP-BPUTZDHNSA-N 0.000 claims description 5
LTUVYLVIZHJCOQ-KKUMJFAQSA-N Glu-Arg-Phe Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O LTUVYLVIZHJCOQ-KKUMJFAQSA-N 0.000 claims description 5
WVWZIPOJECFDAG-AVGNSLFASA-N Glu-Phe-Cys Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CCC(=O)O)N WVWZIPOJECFDAG-AVGNSLFASA-N 0.000 claims description 5
PMNHJLASAAWELO-FOHZUACHSA-N Gly-Asp-Thr Chemical compound [H]NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O PMNHJLASAAWELO-FOHZUACHSA-N 0.000 claims description 5
UQJNXZSSGQIPIQ-FBCQKBJTSA-N Gly-Gly-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)CNC(=O)CN UQJNXZSSGQIPIQ-FBCQKBJTSA-N 0.000 claims description 5
UESJMAMHDLEHGM-NHCYSSNCSA-N Gly-Ile-Leu Chemical compound NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(O)=O UESJMAMHDLEHGM-NHCYSSNCSA-N 0.000 claims description 5
GBYYQVBXFVDJPJ-WLTAIBSBSA-N Gly-Tyr-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)CN)O GBYYQVBXFVDJPJ-WLTAIBSBSA-N 0.000 claims description 5
SKYULSWNBYAQMG-IHRRRGAJSA-N His-Leu-Arg Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O SKYULSWNBYAQMG-IHRRRGAJSA-N 0.000 claims description 5
IIKJNQWOQIWWMR-CIUDSAMLSA-N Leu-Cys-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CS)NC(=O)[C@H](CC(C)C)N IIKJNQWOQIWWMR-CIUDSAMLSA-N 0.000 claims description 5
IASQBRJGRVXNJI-YUMQZZPRSA-N Leu-Cys-Gly Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)NCC(O)=O IASQBRJGRVXNJI-YUMQZZPRSA-N 0.000 claims description 5
YWKNKRAKOCLOLH-OEAJRASXSA-N Leu-Phe-Thr Chemical compound CC(C)C[C@H](N)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)O)C(O)=O)CC1=CC=CC=C1 YWKNKRAKOCLOLH-OEAJRASXSA-N 0.000 claims description 5
ZJZNLRVCZWUONM-JXUBOQSCSA-N Leu-Thr-Ala Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(O)=O ZJZNLRVCZWUONM-JXUBOQSCSA-N 0.000 claims description 5
DIBZLYZXTSVGLN-CIUDSAMLSA-N Lys-Ser-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O DIBZLYZXTSVGLN-CIUDSAMLSA-N 0.000 claims description 5
108010079364 N-glycylalanine Proteins 0.000 claims description 5
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
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- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

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Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Wood Science & Technology (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Gastroenterology & Hepatology (AREA)
Pharmacology & Pharmacy (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Animal Behavior & Ethology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Immunology (AREA)
Chemical Kinetics & Catalysis (AREA)
Hematology (AREA)
Epidemiology (AREA)
Toxicology (AREA)
General Chemical & Material Sciences (AREA)
Diabetes (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

DK198903575A 1988-07-20 1989-07-19 Flagermusspyt-plasminogenaktivatorer DK176140B1 (da)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date
US22169788A	1988-07-20	1988-07-20
US22169788		1988-07-20
US37722189A	1989-07-13	1989-07-13
US37722189		1989-07-13

Publications (3)

Publication Number	Publication Date
DK357589D0 DK357589D0 (da)	1989-07-19
DK357589A DK357589A (da)	1990-01-23
DK176140B1 true DK176140B1 (da)	2006-09-25

Family

ID=26916041

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK198903575A DK176140B1 (da)	1988-07-20	1989-07-19	Flagermusspyt-plasminogenaktivatorer

Country Status (17)

Country	Link
US (1)	US5830849A (de)
EP (1)	EP0352119B1 (de)
JP (1)	JP2713467B2 (de)
KR (1)	KR0138997B1 (de)
AT (1)	ATE126269T1 (de)
AU (1)	AU621389B2 (de)
CA (1)	CA1341090C (de)
DE (1)	DE68923741T2 (de)
DK (1)	DK176140B1 (de)
ES (1)	ES2076965T3 (de)
FI (1)	FI100403B (de)
GR (1)	GR3017587T3 (de)
IE (1)	IE69054B1 (de)
IL (1)	IL91059A (de)
NO (2)	NO302954B1 (de)
NZ (1)	NZ230017A (de)
PT (1)	PT91236B (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
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JPH01500322A (ja) *	1986-03-28	1989-02-09	クリエイティブ　バイオモレクレス，インコーポレーテッド	組織プラスミノーゲンアクテイベーター類縁蛋白質
EP0383417B1 (de) *	1989-02-13	1995-12-27	Schering Aktiengesellschaft	Fledermausspeichel-Plasminogenaktivator vPA-alpha 1
US6008019A (en) *	1989-02-13	1999-12-28	Schering Aktiengesellschaft	Plasminogen activator from saliva of the vampire bat
DE3943241A1 (de) *	1989-12-22	1991-06-27	Schering Ag	Thrombolytisch wirkendes kombinationspraeparat
EP0455424A3 (en)	1990-05-02	1992-04-29	Merck & Co. Inc.	Mammalian inducible promoter cascade system
DK0580685T3 (da) *	1991-04-16	1996-03-04	Boehringer Mannheim Gmbh	Farmaceutisk emballageenhed indeholdende plasminogenaktivatorer til flerfoldig bolusindgift
US5910481A (en) *	1995-11-13	1999-06-08	Immuno Ag	Hybrid proteins with modified activity
US5945432A (en) *	1995-12-22	1999-08-31	The University Of Vermont And State Agricultural College	Thrombolytic agents and thienopyridine derivatives in acute stroke
US5731186A (en) *	1996-02-05	1998-03-24	Schering Aktiengesellschaft	Method for the production of rDSPA α1
DE10153601A1 (de) *	2001-11-02	2003-05-22	Paion Gmbh	DSPA zur Behandlung von Schlaganfall
US20060135425A1 (en) *	2003-05-02	2006-06-22	Paion Deutschland Gmbh	Intravenous injection of plasminogen non-neurotoxic activators for treating cerebral stroke
BRPI0409865A (pt) *	2003-05-02	2006-05-16	Paion Deutschland Gmbh	injeção intravenosa de ativadores de plasminogênio não neurotóxicos para o tratamento do ataque de apoplexia
AU2003227721A1 (en) *	2003-05-02	2004-11-23	Paion Gmbh	Intravenous injection of non-neurotoxic plasminogen activators for the treatment of stroke
DE10342518A1 (de) *	2003-09-12	2005-05-12	Paion Gmbh	Plasminogen-Aktivatoren mit verringerter Lysin-Bindungskapazität
AR068914A1 (es) *	2007-10-18	2009-12-16	Paion Deutschland Gmbh	Uso de un activador de plasminogeno para la preparacion de un medicamento para el tratamiento de apoplejia
EP2289542A1 (de)	2009-08-31	2011-03-02	PAION Deutschland GmbH	Behandlung von neurologischen und neurodegenerativen Erkrankungen
EP2289541A1 (de)	2009-08-31	2011-03-02	PAION Deutschland GmbH	Behandlung von neurologischen und neurodegenerativen Erkrankungen
JP7360945B2 (ja)	2017-02-03	2023-10-13	アクティコーバイオテック	抗ヒトｇｐｖｉ抗体を用いた血小板凝集の阻害
WO2018210860A1 (en)	2017-05-16	2018-11-22	INSERM (Institut National de la Santé et de la Recherche Médicale)	Methods and pharmaceutical compositions for the treatment of acute ischemic stroke
US12123000B2 (en)	2018-06-15	2024-10-22	Inserm (Institut National De La Sante Et De La Recherche Medicale)	Use of PI3KC2B inhibitors for the preservation of vascular endothelial cell barrier integrity
MY209917A (en) *	2019-02-05	2025-08-12	Dyno Nobel Asia Pacific Pty Ltd	Phase-stabilized ammonium nitrate prills and related products and methods
WO2021148439A1 (en)	2020-01-21	2021-07-29	INSERM (Institut National de la Santé et de la Recherche Médicale)	Methods for stimulating cerebrovascular function
EP4161579A1 (de)	2020-06-09	2023-04-12	Institut National de la Santé et de la Recherche Médicale (INSERM)	Fucoidanfunktionalisierte polysaccharidpartikel mit t-pa zur gezielten thrombolytischen therapie
EP4482863A1 (de)	2022-02-21	2025-01-01	Acticor Biotech	Behandlung von herz-kreislauf-erkrankungen mit anti-humanen gpvi-antikörpern
EP4556492A1 (de)	2023-11-14	2025-05-21	Acticor Biotech	Behandlung von herz-kreislauf-erkrankungen mit anti-humanen gpvi-antikörpern in subpopulationen von personen

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4766075A (en) *	1982-07-14	1988-08-23	Genentech, Inc.	Human tissue plasminogen activator
US5010002A (en) *	1983-01-19	1991-04-23	Genentech, Inc.	Human t-PA production using vectors coding DHFR protein
WO1987005934A1 (en) *	1986-03-28	1987-10-08	Roberto Crea	Protein analogues of tissue plasminogen activator
ZA887556B (en) *	1987-10-09	1989-07-26	Monsanto Co	Modified tissue plasminogen activator
EP0383417B1 (de) *	1989-02-13	1995-12-27	Schering Aktiengesellschaft	Fledermausspeichel-Plasminogenaktivator vPA-alpha 1

1989
- 1989-07-19 DK DK198903575A patent/DK176140B1/da not_active IP Right Cessation
- 1989-07-19 IE IE235489A patent/IE69054B1/en not_active IP Right Cessation
- 1989-07-19 FI FI893500A patent/FI100403B/fi active IP Right Grant
- 1989-07-20 ES ES89307411T patent/ES2076965T3/es not_active Expired - Lifetime
- 1989-07-20 NZ NZ230017A patent/NZ230017A/en unknown
- 1989-07-20 PT PT91236A patent/PT91236B/pt not_active IP Right Cessation
- 1989-07-20 AT AT89307411T patent/ATE126269T1/de not_active IP Right Cessation
- 1989-07-20 JP JP1188673A patent/JP2713467B2/ja not_active Expired - Lifetime
- 1989-07-20 CA CA000606185A patent/CA1341090C/en not_active Expired - Lifetime
- 1989-07-20 DE DE68923741T patent/DE68923741T2/de not_active Expired - Lifetime
- 1989-07-20 IL IL9105989A patent/IL91059A/en unknown
- 1989-07-20 KR KR1019890010264A patent/KR0138997B1/ko not_active Expired - Fee Related
- 1989-07-20 NO NO892976A patent/NO302954B1/no not_active IP Right Cessation
- 1989-07-20 EP EP89307411A patent/EP0352119B1/de not_active Expired - Lifetime
- 1989-07-25 AU AU38915/89A patent/AU621389B2/en not_active Expired
1995
- 1995-06-06 US US08/467,966 patent/US5830849A/en not_active Expired - Lifetime
- 1995-09-29 GR GR950402700T patent/GR3017587T3/el unknown
1997
- 1997-02-10 NO NO19970603A patent/NO314548B1/no not_active IP Right Cessation

Also Published As

Publication number	Publication date
FI893500L (fi)	1990-01-21
EP0352119A3 (en)	1990-07-04
PT91236B (pt)	1995-03-01
NZ230017A (en)	1992-05-26
AU621389B2 (en)	1992-03-12
DE68923741T2 (de)	1996-02-15
AU3891589A (en)	1990-01-25
CA1341090C (en)	2000-08-29
IE892354L (en)	1990-01-20
KR910003094A (ko)	1991-02-26
ATE126269T1 (de)	1995-08-15
DK357589A (da)	1990-01-23
IE69054B1 (en)	1996-08-07
NO970603L (no)	1990-01-22
NO892976D0 (no)	1989-07-20
FI100403B (fi)	1997-11-28
NO314548B1 (no)	2003-04-07
PT91236A (pt)	1990-02-08
ES2076965T3 (es)	1995-11-16
EP0352119A2 (de)	1990-01-24
US5830849A (en)	1998-11-03
IL91059A0 (en)	1990-02-09
NO302954B1 (no)	1998-05-11
NO892976L (no)	1990-01-22
EP0352119B1 (de)	1995-08-09
KR0138997B1 (ko)	1998-04-30
NO970603D0 (no)	1997-02-10
DK357589D0 (da)	1989-07-19
DE68923741D1 (de)	1995-09-14
GR3017587T3 (en)	1995-12-31
FI893500A0 (fi)	1989-07-19
JPH02167075A (ja)	1990-06-27
IL91059A (en)	1995-06-29
JP2713467B2 (ja)	1998-02-16

Legal Events

Date	Code	Title	Description
2009-08-03	PUP	Patent expired

Publication	Publication Date	Title
FI100403B (fi)	1997-11-28	Menetelmä glykosyloitujen tai glykosyloimattomien vampyyrilepakon sylj en plasminogeeniaktivaattoreiden valmistamiseksi
FI105202B (fi)	2000-06-30	Menetelmä aktivoitavien fibrinolyyttisten ja veren hyytymistä estävien plasminogeenianalogien valmistamiseksi
JP4163517B2 (ja)	2008-10-08	フォンビルブラント因子（ｖＷＦ）切断酵素
EP0771211A4 (de)	1997-11-12	Präparate und verfahren zur arzneistoffverabreichung durch blutplättchen zur behandlung von kardiovaskulären erkrankungen
JPH05508150A (ja)	1993-11-18	酸化抵抗性トロンボモジュリン類縁体
JPH10513062A (ja)	1998-12-15	細胞質アンチプロテイナーゼ−２及び細胞質アンチプロテイナーゼ−３並びにコーディング配列
NO314848B1 (no)	2003-06-02	Protein for inhibering av kollagenfremkalt blodplateaggregering, DNA som koder for proteinet, vektor som omfatter et slikt DNA, vertscelletransformert medvektoren, fremgangsmåter for fremstilling og rensing avproteinet, farmasöytiskpreparat
NO308708B1 (no)	2000-10-16	FremgangsmÕte for fremstilling av et trombolytisk protein, samt isolert DNA, mikroorganisme og fremgangsmÕte for fremstilling av et farmasøytisk preparat
RU2143490C1 (ru)	1999-12-27	Бифункциональный вариант урокиназы, плазмида, содержащая синтетический структурный ген, кодирующий бифункциональную урокиназу, плазмида (варианты), способ получения плазмиды, способ получения бифункционального варианта урокиназы, тромболитическое средство
JPH08511157A (ja)	1996-11-26	Ｘａファクター阻害活性を有する新規ポリペプチド
NZ229691A (en)	1992-03-26	A t-pa analogue with at least one amino acid of native t-pa substituted for another between position 67 and 72
DK175369B1 (da)	2004-09-13	Anvendelse af tPA-varianter til fremstilling af et terapeutisk præparat til behandling af vaskulæresygdomme
EP0395375B1 (de)	1996-08-21	Für Ancrod-ähnliche Polypeptide kodierende DNA-Sequenzen und deren Expressionsprodukte enthaltende Zusammensetzungen
CA1341318C (en)	2001-11-13	Dna clones of human placental plasminogen activator inhibitor
JPH08231595A (ja)	1996-09-10	繊維素溶解性のトロンビン阻害性質を有するキメラたん白質
US5801017A (en)	1998-09-01	Production of recombinant factor Xa inhibitor of leech Hirudo medicinalis
CA2083286C (en)	2001-11-27	Recombinant fibrinogenases, preparation and use thereof
EP0712934A2 (de)	1996-05-22	Proteine mit fibrinolytischen und gerinnungshemmenden Eigenschaften
FI100406B (fi)	1997-11-28	Menetelmä vampyyrilepakon syljen plasminogeeniaktivaattoreiden valmist amiseksi
HU211335A9 (en)	1995-11-28	New polipeptide compounds
HK1010111A (en)	1999-06-11	Chimera proteins with fibrinolytic and coagulation inhibiting characteristics