DK2069389T3 - Glucanaser, nucleinsyrer, som koder for dem, og fremgangsmåder til fremstilling og anvendelse af disse - Google Patents
Glucanaser, nucleinsyrer, som koder for dem, og fremgangsmåder til fremstilling og anvendelse af disse Download PDFInfo
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- DK2069389T3 DK2069389T3 DK07875173.2T DK07875173T DK2069389T3 DK 2069389 T3 DK2069389 T3 DK 2069389T3 DK 07875173 T DK07875173 T DK 07875173T DK 2069389 T3 DK2069389 T3 DK 2069389T3
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Claims (19)
1. Isoleret, syntetisk eller rekombinant nucleinsyre omfattende (a) en nucleinsyresekvens med mindst 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% eller 100% sekvensidentitet med en nucleinsyresekvens fra SEQ ID NO:1, hvor nucleinsyren koder for mindst et polypeptid med en gluca-naseaktivitet, og som bevarer mindst 75% restaktivitet efter 2 minutter ved 95°C, og nucleinsyresekvensen omfatter mindst de følgende nucleotidgruppe-modifikationer A) til C) baseret på sekvensen i SEQ ID NO: 1: A) modifikation ved positionerne 181 til 183 valgt fra: nucleotiderne ved positionerne 181 til 183 er CAA eller CAG, nucleotiderne ved positionerne 181 til 183 er TCT, TCC, TCA, TCG, AGT eller AGC, B) modifikation ved positionerne 571 til 573 valgt fra: nucleotiderne ved positionerne 571 til 573 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 571 til 573 er TGT eller TGC, nucleotiderne ved positionerne 571 til 573 er TTA, TTG, CTT, CTC, CTA eller CTG, C) modifikation ved positionerne 826 til 828 valgt fra: nucleotiderne ved positionerne 826 til 828 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 826 til 828 er TGT eller TGC, nucleotiderne ved positionerne 826 til 828 er TCT, TCC, TCA, TCG, AGT eller AGC; og eventuelt er sekvensidentiteterne bestemt ved analyse med en sekvenssammenligningsalgoritme eller ved virtuel inspektion, og eventuelt er sekvenssammenligningsalgoritmen en BLAST version 2.2.2-algelleritme, hvor filterindstillingen er sat til blastall-p blastp-d ”nr pataa” -FF, og alle andre muligheder er sat til default, (b) nucleinsyren i (a), hvor nucleinsyren omfatter sekvensen i SEQ ID NO:6, SEQ ID NO: 8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:18, SEQ ID NO:20 eller SEQ ID NO:22, (c) nucleinsyresekvensen (polynucleotidet) fra (a) eller (b), som koder for et po-lypeptid med en glucanaseaktivitet, men som mangler: en signalsekvens eller proproteinsekvens, eller en homolog promotersekvens, (d) nucleinsyren (polynucleotidet) fra (a), (b) eller (c), som koder for et polypep-tid med en glucanaseaktivitet og yderligere omfatter en heterolog aminosyrese-kvens, eller nucleinsyren (polynucleotidet) fra (a), (b) eller (c) omfatter en heterolog nucleotidsekvens, (e) nucleinsyren (polynucleotidet) fra (d), hvor den heterologe aminosyrese-kvens omfatter eller består af en sekvens, som koder for en heterolog (leader) signalsekvens, eller en tag eller en epitop, eller den heterologe nucleotidsekvens omfatter en heterolog promotersekvens, (f) nucleinsyren (polynucleotidet) fra (d) eller (e), hvor den heterologe nucleotidsekvens koder for en heterolog (leader) signalsekvens omfattende eller bestående af en N-terminal og/eller C-terminal forlængelse til målsætning til et endoplasmatisk reticulum (ER) eller endomembran, eller til et majs-endoplasmatisk reticulum (ER) eller endomembransystem, eller den heterologe sekvens koder for et restriktionssted, (g) nucleinsyren (polynucleotidet) fra (e), hvor den heterologe promotersekvens omfatter eller består af en konstitutiv eller induceringsdygtig promoter, eller en celletype specifik promoter, eller en plantespecifik promoter, eller en majsspecifik promoter, (h) nucleinsyren (polynucleotidet) fra en vilkårlig af (a) til (g), hvor glucanaseak-tiviteten omfatter: en endoglucanaseaktivitet, katalyserende hydrolyse af 1,4-be-ta-D-glycosidbindinger eller interne β-1,3-glucosidbindinger, hydrolyse afen glu-can, en mannan, en arabinoxylan eller an xylan til at fremstille et polysaccharid eller oligomer med mindre molekylvægt, en endo-1,4-beta-endoglucanaseakti-vitet, en 1,4-beta-D-glycosidbindingsaktivitet omfattende hydrolyse af en 1,4-beta-D-glycosidbinding i en cellulose, et cellulosederivat, et lichenin eller et ce-realie, eller et cellulosederivat omfattende en carboxymethylcellulose eller en hydroxyethylcellulose, eller et cerealie omfattende en beta-D-glucan eller en xy-loglucan, hydrolyserende polysaccharider omfattende 1,4-p-glycosidforbundne D-glucopyranoser, hydrolyserende en cellulose, et cellulosederivat eller en he-micellulose, hydrolyserende en cellulose eller en hemicellulose i træpulp eller papirpulp eller et træ- eller papirprodukt, catalyserende hydrolyse af glucan i et foderstof, et fødevareprodukt eller en drikkevare, eller et foder, fødevareprodukt eller drikkevare omfattende et cerealiebaseret dyrefoder, en ølurt eller en øl, en dej, en frugt eller en grøntsag, catalyserende hydrolyse af en glucan, en mannan, en arabinoxylan eller en xylan, i en mikrobiel celle, en fungal celle, en pattedyrscelle eller en plantecelle, (i) isoleret, syntetisk eller rekombinant nucleinsyre ifølge et vilkårligt af (a) til (h), hvor nucleotidremanensen i et kryptisk transcriptionsmæssigt startsted er modificeret til at eliminere det meste eller al produktion fra et trunkeret transcript, (j) isoleret, syntetisk eller rekombinant nucleinsyre fra (i), hvor nucleotidgruppe-modifikationerne i det kryptiske transcriptionelle startsted omfatter en ændring i et ribosombindingssted (RBS), (k) isoleret, syntetisk eller rekombinant nucleinsyre fra (i) eller (j), hvor nucleo-tidgruppemodifikationerne i det kryptiske transcriptionelle startsted omfatter følgende modifikationer i grupperne 77 til 106 i SEQ ID NO:1 til: ATGAGGGCG-ACTGGGGAGTCGTGATAAAAG, eller ækvivalent, eller (l) en nucleinsyresekvens fuldstændig komplementær til nucleotidsekvensen ifølge et vilkårligt af (a) til (k).
2. Isoleret, syntetisk eller rekombinant nucleinsyresekvens ifølge krav 1, som yderligere omfatter mindst en af de følgende yderligere nucleotidgruppeændrin-ger baseret på sekvensen af SEQ ID NO: 1, og valgt fra: nucleotiderne ved positionerne 4 til 6 er AAT eller AAC, nucleotiderne ved positionerne 37 til 39 er AAT eller AAC, nucleotiderne ved positionerne 112 til 114 er TAT eller TAC, nucleotiderne ved positionerne 169 til 171 er GAT eller GAC, nucleotiderne ved positionerne 184 til 186 er ACT, ACC, ACA eller ACG, nucleotiderne ved positionerne 187 til 189 er CAT eller CAC, nucleotiderne ved positionerne 187 til 189 er ACT, ACC, ACA eller ACG, nucleotiderne ved positionerne 205 til 207 er GAA eller GAG, nucleotiderne ved positionerne 205 til 207 er CAT eller CAC, nucleotiderne ved positionerne 205 til 207 er CAA eller CAG, nucleotiderne ved positionerne 205 til 207 er TCT, TCC, TCA, TCG, AGT eller AGC, nucleotiderne ved positionerne 205 til 207 er TAT eller TAC, nucleotiderne ved positionerne 208 til 210 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 211 til 213 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 211 til 213 er GAA eller GAG, nucleotiderne ved positionerne 211 til 213 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 211 til 213 er CAA eller CAG, nucleotiderne ved positionerne 211 til 213 er TCT, TCC, TCA, TCG, AGT eller AGC, nucleotiderne ved positionerne 211 til 213 er ACT, ACC, ACA eller ACG, nucleotiderne ved positionerne 220 til 222 er GAA eller GAG, nucleotiderne ved positionerne 220 til 222 er TTA, TTG, CTT, CTC, CTA eller CTG, nucleotiderne ved positionerne 220 til 222 er ATG, nucleotiderne ved positionerne 280 til 282 er CAA eller CAG, nucleotiderne ved positionerne 301 til 303 er TAT eller TAC, nucleotiderne ved positionerne 307 til 309 erTGT eller TGC, nucleotiderne ved positionerne 307 til 309 er CAA eller CAG, nucleotiderne ved positionerne 316 til 318 er GGT, GGC, GGA eller GGG, nucleotiderne ved positionerne 325 til 327 er TTA, TTG, CTT, CTC, CTA eller CTG, nucleotiderne ved positionerne 346 til 348 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 346 til 348 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 388 til 390 er TAT eller TAC, nucleotiderne ved positionerne 391 til 393 er TTA, TTG, CTT, CTC, CTA eller CTG, nucleotiderne ved positionerne 442 til 444 er CAT eller CAC, nucleotiderne ved positionerne 484 til 486 er CAA eller CAG, nucleotiderne ved positionerne 496 til 498 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 496 til 498 er GTT, GTC, GTA eller GTG, nucleotiderne ved positionerne 547 til 549 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 547 til 549 er GTT, GTC, GTA eller GTG, nucleotiderne ved positionerne 556 til 558 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 556 til 558 er GAT eller GAC, nucleotiderne ved positionerne 556 til 558 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 556 til 558 er TCT, TCC, TCA, TCG, AGT eller AGC, nucleotiderne ved positionerne 601 til 603 er ATT, ATC eller ATA, nucleotiderne ved positionerne 601 til 603 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 601 til 603 er GTT, GTC, GTA eller GTG, nucleotiderne ved positionerne 634 til 636 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 646 til 648 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 688 til 690 er AAA eller AAG, nucleotiderne ved positionerne 688 til 690 er CAA eller CAG, nucleotiderne ved positionerne 688 til 690 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 691 til 693 er ATT, ATC eller ATA, nucleotiderne ved positionerne 691 til 693 er ATG, nucleotiderne ved positionerne 691 til 693 er GTT, GTC, GTA eller GTG, nucleotiderne ved positionerne 700 til 702 er GAT eller GAC, nucleotiderne ved positionerne 736 til 738 er CAA eller CAG, nucleotiderne ved positionerne 736 til 738 er TCT, TCC, TCA, TCG, AGT eller AGC, nucleotiderne ved positionerne 772 til 774 er TCT, TCC, TCA, TCG, AGT eller AGC, nucleotiderne ved positionerne 772 til 774 er TAT eller TAC, nucleotiderne ved positionerne 784 til 786 er CAT eller CAC, nucleotiderne ved positionerne 784 til 786 er ATG, nucleotiderne ved positionerne 784 til 786 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 784 til 786 er CAA eller CAG, nucleotiderne ved positionerne 808 til 810 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 811 til 813 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 829 til 831 er TCT, TCC, TCA, TCG, AGT eller AGC, nucleotiderne ved positionerne 838 til 840 er GGT, GGC, GGA eller GGG, nucleotiderne ved positionerne 868 til 870 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 889 til 891 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 889 til 891 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 892 til 894 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 892 til 894 er AAT eller AAC, nucleotiderne ved positionerne 892 til 894 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 892 til 894 er TCT, TCC, TCA, TCG, AGT eller AGC, nucleotiderne ved positionerne 892 til 894 er GTT, GTC, GTA eller GTG, nucleotiderne ved positionerne 898 til 900 er GGT, GGC, GGA eller GGG, nucleotiderne ved positionerne 901 til 903 er CAA eller CAG, nucleotiderne ved positionerne 913 til 915 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 934 til 936 er ATT, ATC eller ATA, og/eller nucleotiderne ved positionerne 943 til 945 er ATT, ATC eller ATA.
3. Isoleret, syntetisk eller rekombinant nucleinsyre ifølge krav 1, hvor nuclein- syresekvensen omfatter en sekvensmodifikvedion af SEQ ID NO: 1, og (a) modifikvedionerne omfatter eller består af følgende ændringer: nucleotiderne ved positionerne 112 til 114 er TATeller TAC, nucleotiderne ved positionerne 181 til 183 er CAA eller CAG, nucleotiderne ved positionerne 205 til 207 er GAA eller GAG, nucleotiderne ved positionerne 280 til 282 er CAA eller CAG, nucleotiderne ved positionerne 547 til 549 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 571 til 573 er GCT, GCC, GCA eller GCG, og nucleotiderne ved positionerne 826 til 828 er GCT, GCC, GCA eller GCG; (b) modifikvedionen omfatter eller består af følgende ændringer: nucleotiderne ved positionerne 112 til 114 er TAT eller TAC, nucleotiderne ved positionerne 181 til 183 er CAA eller CAG, nucleotiderne ved positionerne 205 til 207 er GAA eller GAG, nucleotiderne ved positionerne 280 til 282 er CAA eller CAG, nucleotiderne ved positionerne 496 til 498 er GTT, GTC, GTA eller GTG, nucleotiderne ved positionerne 547 til 549 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 571 til 573 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 634 til 636 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 826 til 828 er GCT, GCC, GCA eller GCG, og nucleotiderne ved positionerne 838 til 840 er GGT, GGC, GGA eller GGG; (c) modifikvedionen omfatter eller består af følgende ændringer: nucleotiderne ved positionerne 112 til 114 er TAT eller TAC, nucleotiderne ved positionerne 181 til 183 er CAA eller CAG, nucleotiderne ved positionerne 205 til 207 er GAA eller GAG, nucleotiderne ved positionerne 280 til 282 er CAA eller CAG, nucleotiderne ved positionerne 496 til 498 er GTT, GTC, GTA eller GTG, nucleotiderne ved positionerne 547 til 549 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 571 til 573 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 634 til 636 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 826 til 828 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 838 til 840 er GGT, GGC, GGA eller GGG, og nucleotiderne ved positionerne 889 til 891 er CCA, CCC, CCG eller CCT; (d) modifikvedionen omfatter eller består af følgende ændringer: nucleotiderne ved positionerne 181 til 183 er CAA eller CAG, nucleotiderne ved positionerne 205 til 207 er GAA eller GAG, nucleotiderne ved positionerne 280 til 282 er CAA eller CAG, nucleotiderne ved positionerne 496 til 498 er GTT, GTC, GTA eller GTG, nucleotiderne ved positionerne 547 til 549 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 571 til 573 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 634 til 636 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 826 til 828 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 838 til 840 er GGT, GGC, GGA eller GGG, nucleotiderne ved positionerne 889 til 891 er CCA, CCC, CCG eller CCT, og nucleotiderne ved positionerne 901 til 903 er CAA eller CAG; (e) modifikvedionen omfatter eller består af følgende ændringer: nucleotiderne ved positionerne 181 til 183 er CAA eller CAG, nucleotiderne ved positionerne 205 til 207 er GAA eller GAG, nucleotiderne ved positionerne 211 til 213 er TCT, TCC, TCA, TCG, AGT eller AGC, nucleotiderne ved positionerne 280 til 282 er CAA eller CAG, nucleotiderne ved positionerne 496 til 498 er GTT, GTC, GTA eller GTG, nucleotiderne ved positionerne 547 til 549 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 571 til 573 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 634 til 636 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 826 til 828 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 838 til 840 er GGT, GGC, GGA eller GGG, nucleotiderne ved positionerne 889 til 891 er CCA, CCC, CCG eller CCT, og nucleotiderne ved positionerne 901 til 903 er CAA eller CAG; (f) modifikvedionen omfatter eller består af følgende ændringer: nucleotiderne ved positionerne 181 til 183 er CAA eller CAG, nucleotiderne ved positionerne 205 til 207 er GAA eller GAG, nucleotiderne ved positionerne 208 til 210 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 211 til 213 er TCT, TCC, TCA, TCG, AGT eller AGC, nucleotiderne ved positionerne 496 til 498 er GTT, GTC, GTA eller GTG, nucleotiderne ved positionerne 547 til 549 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 571 til 573 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 634 til 636 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 826 til 828 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 838 til 840 er GGT, GGC, GGA eller GGG, nucleotiderne ved positionerne 889 til 891 er CCA, CCC, CCG eller CCT, og nucleotiderne ved positionerne 901 til 903 er CAA eller CAG; eller (g) modifikvedionen omfatter eller består af følgende ændringer: nucleotiderne ved positionerne 112 til 114 er TAT eller TAC, nucleotiderne ved positionerne 181 til 183 er CAA eller CAG, nucleotiderne ved positionerne 205 til 207 er GAA eller GAG, nucleotiderne ved positionerne 211 til 213 er TCT, TCC, TCA, TCG, AGT eller AGC, nucleotiderne ved positionerne 496 til 498 er GTT, GTC, GTA eller GTG, nucleotiderne ved positionerne 547 til 549 er CGT, CGC, CGA, CGG, AGA eller AGG, nucleotiderne ved positionerne 571 til 573 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 634 til 636 er CCA, CCC, CCG eller CCT, nucleotiderne ved positionerne 691 til 693 er ATT, ATC eller ATA, nucleotiderne ved positionerne 826 til 828 er GCT, GCC, GCA eller GCG, nucleotiderne ved positionerne 838 til 840 er GGT, GGC, GGA eller GGG, nucleotiderne ved positionerne 889 til 891 er CCA, CCC, CCG eller CCT, OG nucleotiderne ved positionerne 901 til 903 er CAA eller CAG.
4. Isoleret, syntetisk eller rekombinant nucleinsyre ifølge krav 1, hvor nuclein-syresekvensen koder for et polypeptid med aminosyresekvensen: (a) fra SEQ ID NO:2 og aminosyresekvensen omfatter følgende aminosyre-gruppeændringer A) til C) til SEQ ID NO:2: A) en aminosyreændring valgt fra: tyrosin ved aminosyreposition 61 erglutamin, tyrosin ved aminosyreposition 61 er serin, B) en aminosyreændring valgt fra: serin ved aminosyreposition 191 eralanin, serin ved aminosyreposition 191 er cystein, serin ved aminosyreposition 191 er leucin, C) en aminosyreændring valgt fra: methionin ved aminosyreposition 276 er alanin, methionin ved aminosyreposition 276 er cystein, methionin ved aminosyreposition 276 er serin.
5. Ekspressionskassette, vektor eller kloningsvehikel omfattende (a) nuclein-syresekvensen ifølge et vilkårligt af kravene 1 til 4, (b) ekspressionskassette, vektor eller kloningsvehikel fra (a) omfattende eller inserteret i en viral vektor, et plasmid, en phag, et phagemid, et cosmid, et fosmid, en baiteriophag eller et kunstigt kromosom, (c) ekspressionskassette, vektor eller kloningsvehikel fra (b), hvor den virale vektor omfatter en adenovirusvektor, en retroviral vektor eller en adenoassocieret viral vektor, eller (d) ekspressionskassetten, vektoren eller kloningsvehiklen fra (a), (b) eller (c) omfattende eller inserteret i et kunstigt bakterielt kromosom (BAC), et plasmid, et bakteriophag P1-afledt vektor (PAC), et kunstigt gærkromosom (YAC) eller et kunstigt pattedyrskromosom (MAC).
6. Transformeret celle, plante eller frø: (a) omfattende en nucleinsyre omfattende sekvensen ifølge et af kravene 1 til 4, (b) omfattende ekspressionskassetten, en vektor eller en kloningsvehikel ifølge krav 5, (c) den transformerede celle fra (a) eller (b), hvor cellen er en bakteriel celle, en pattedyrscelle, en fungal celle eller en gærcelle, en insektcelle eller en plantecelle, (d) den transformerede celle ifølge (c), hvor den bakterielle celle er en vilkårlig art inden for slægten Escherichia, Bacillus, Streptomyces, Salmonella, Pseudomonas eller Staphylococcus, eller Escherichia coli, Lactilcoccus lactis, Bacillus subtilis, Bacillus cereus, Salmonella typhimurium eller Pseudomonas fluorescens, eller (e) den transgene plante eller frø ifølge (a) eller (b), hvor planten er en majsplante, en sorghumplante, en kartoffelplante, en tomatplante, en hvedeplante, en oliefrøsplante, en rapsfrøplante, en sojabønneplante, en risplante, en bygplante, et græs, en tobaksplante, eller en foder- og/eller foderstofplante for et dyr, eller en drøvtygger, og eventuelt er foderet eller foderplanten hø, majs, hirse, soja, hvede, boghvede, byg, lucerne, rug, en årsgræs, sorghum, sudan-græs, veldtgræs eller bøffelgræs eller hvor frøet er et majsfrø, en hvedekerne et oliefrø, et rapsfrø, et sojabønnefrø, en palmekerne, et solsikkefrø, et sesamfrø, ris, byg, jordnød eller et tobaksplantefrø, eller et frø fra et vilkårligt foder og/eller foderplante til et dyr eller en drøvtygger, og eventuelt er foderet eller foderplanten hø, majs, hirse, soja, hvede, boghvede, byg, lucerne, rug, et årsgræs, sorghum, sudangræs, veldtgræs eller bøffelgræs.
7. Transgent ikke-humant dyr: (a) omfattende sekvensen ifølge et vilkårligt af kravene 1 til 4, eller ekspressionskassetten, en vektor eller en kloningsvehikel ifølge krav 5, eller (b) det transgene ikke-humane dyr fra (a), hvor dyret er en mus.
8. Isoleret, syntetisk eller rekombinant polypeptid med en glucanaseaktivitet, og som bevarer mindst 75% restaktivitet efter 2 minutter ved 95°C, hvilket polypeptid omfatter: (a) (i) en aminosyresekvens kodet for af en nucleinsyre omfattende eller bestående af nucleinsyresekvensen ifølge et vilkårligt af kravene 1 til 4, eller (ii) med en aminosyresekvens som anført i SEQ ID NO:2 og omfattende følgende ami-nosyregruppeændringer A) til C) til SEQ ID NO:2: A) en aminosyreændring valgt fra: tyrosin ved aminosyreposition 61 erglutamin, tyrosin ved aminosyreposition 61 er serin, B) aminosyreændring valgt fra: serin ved aminosyreposition 191 er alanin, serin ved aminosyreposition 191 er cystein, serin ved aminosyreposition 191 er leucin, og C) aminosyreændring valgt fra: methionin ved aminosyreposition 276 er alanin, methionin ved aminosyreposition 276 er cystein, methionin ved aminosyreposition 276 er serin, (b) polypeptidet ifølge (a), omfattende aminosyresekvensen ifølge SEQ ID NO:7, SEQ ID NO: 9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:19, SEQ ID NO:21 eller SEQ ID NO:23, (c) polypeptidet (a) eller (b) med en glucanaseaktivitet, men uden: en signalsekvens eller en proproteinsekvens, (d) polypeptid ifølge (a), (b) eller (c) med en glucanaseaktivitet og yderligere omfattende en heterolog sekvens, (e) polypeptidet ifølge (d), hvor den heterologe aminosyresekvens omfatter eller består af en heterolog (leader) signalsekvens, eller en tag eller en epitop, (f) polypeptidet ifølge (d) eller (e), hvor den heterologe (leader) signalsekvens omfatter eller består af en N-terminal og/eller C-terminal forlængelse til målsætning for et endoplasmatisk reticulum (ER) eller endomembran, eller til et majs-endoplasmatisk reticulum (ER) eller endomembransystem, eller den heterologe aminosyresekvens omfatter eller består af et enzymmålsted, (g) polypeptidet ifølge et vilkårligt af (a) til (f), hvor glucanaseaktiviten omfatter: en endoglucanaseaktivitet, katalyserende hydrolyse af 1,4-beta-D-glycosidbin-dinger eller interne β-1,3-glucosidbindinger, hydrolyserende en glucan, mannan, arabinoxylan eller xylan, for at fremstille et polysaccharid eller oligomer med mindre molekylvægt, en endo-1,4-beta-endoglucanaseaktivitet, en 1,4-beta-D-glycosidbindingsaktivitet omfattende hydrolyse af en 1,4-beta-D-glycosidbinding i en cellulose, et cellulosederivat, et lichenin eller et cerealie, eller et cellulosederivat omfattende en carboxymethylcellulose eller en hydroxy-ethylcellulose, eller et cerealie omfattende en beta-D-glucan eller et xyloglucan, hydrolyserende polysaccharider omfattende 1,4-3-glycosidbundet D-glucopyra-noser, hydrolyserende en cellulose, et cellulosederivat eller et hemicellulose, hydrolyserende en cellulose eller et hemicellulose i en træ- eller papirpulp eller et træ- eller papirprodukt, katalyserende hydrolyse af glucan i et foder, et fødevareprodukt eller en drikkevare, eller et foder, fødevareprodukt eller drikkevare omfattende et cerealiebaseret dyrefoder, en ølurt eller en øl, en dej, en frugt eller en grøntsag, katalyserende hydrolyse af en glucan, mannan, arabinoxylan eller xylan, i en mikrobiel celle, fungal celle, pattedyrscelle eller en plantecelle, (h) polypeptidet ifølge en vilkårlig af (a) til (g), hvor (i) polypeptidet er glycosyle-ret, eller polypeptidet omfatter mindst et glycosyleringssted, (ii) polypeptidet iføl ge (i), hvor glycosyleringen er en N-forbundet glycosylering eller en O-forbundet glycosylering, (iii) polypeptidet ifølge (i) eller (ii), hvor polypeptidet er glycosyle-ret efter at være blevet udtrykt i en gærcelle, eller (iv) polypeptidet ifølge (iii), hvor gærcellen er P. pastoris eller S. pombe, eller (i) polypeptidet ifølge et vilkårligt af (a) til (h), hvor (i) polypeptidet yderligere omfatter yderligere aminosyregrupper mellem en signalsekvens (leader sekvens eller leader peptid) og enzymet, eller (ii) polypeptidet ifølge (i), hvor de yderligere aminosyregrupper omfatter Glu-Ala.
9. Isoleret, syntetisk eller rekombinant polypeptid ifølge krav 8 og yderligere omfattende mindst en yderligere aminosyreændring valgt fra: glycin ved aminosyreposition 2 er asparagin, glycin ved aminosyreposition 13 er asparagin, phenylalanin ved aminosyreposition 38 er tyrosin, serin ved aminosyreposition 57 er asparaginsyre, alanin ved aminosyreposition 62 erthreonin, phenylalanin ved aminosyreposition 63 er histidin, phenylalanin ved aminosyreposition 63 er threonin, methionin ved aminosyreposition 69 er glutaminsyre, methionin ved aminosyreposition 69 er glutamin, methionin ved aminosyreposition 69 er histidin, methionin ved aminosyreposition 69 er serin, methionin ved aminosyreposition 69 er tyrosin, asparaginsyre ved aminosyreposition 70 er prolin, arginin ved aminosyreposition 71 er alanin, arginin ved aminosyreposition 71 er glutaminsyre, arginin ved aminosyreposition 71 er glutamin, arginin ved aminosyreposition 71 er prolin, arginin ved aminosyreposition 71 er serin, arginin ved aminosyreposition 71 erthreonin, lysin ved aminosyreposition 74 er glutaminsyre, lysin ved aminosyreposition 74 er leucin, lysin ved aminosyreposition 74 er methionin, eroleucin ved aminosyreposition 94 erglutamin, methionin ved aminosyreposition 101 er tyrosin, asparaginsyre ved aminosyreposition 103 er cystein, asparaginsyre ved aminosyreposition 103 erglutamin, glutaminsyre ved aminosyreposition 106 er glycin, glutaminsyre ved aminosyreposition 109 er leucin, lysin ved aminosyreposition 116 er alanin, lysin ved aminosyreposition 116 er arginin, phenylalanin ved aminosyreposition 130 er tyrosin, phenylalanin ved aminosyreposition 131 er leucin, glutaminsyre ved aminosyreposition 148 er histidin, lysin ved aminosyreposition 162 erglutamin, eroleucin ved aminosyreposition 166 er alanin, eroleucin ved aminosyreposition 166 ervalin, serin ved aminosyreposition 183 er arginin, serin ved aminosyreposition 183 er valin, lysin ved aminosyreposition 186 er alanin, lysin ved aminosyreposition 186 er asparaginsyre, lysin ved aminosyreposition 186 er prolin, lysin ved aminosyreposition 186 er serin, phenylalanin ved aminosyreposition 201 er isoleucin, phenylalanin ved aminosyreposition 201 er prolin, phenylalanin ved aminosyreposition 201 ervalin, glutaminsyre ved aminosyreposition 212 er prolin, lysin ved aminosyreposition 216 er alanin, hertidin ved aminosyreposition 230 er arginin, hertidin ved aminosyreposition 230 erglutamin, hertidin ved aminosyreposition 230 er lysin, leucin ved aminosyreposition 231 er isoleucin, leucin ved aminosyreposition 231 er methionin, leucin ved aminosyreposition 231 ervalin, glutaminsyre ved aminosyreposition 234 er asparaginsyre, lysin ved aminosyreposition 246 er glutamin, lysin ved aminosyreposition 246 er serin, arginin ved aminosyreposition 258 er serin, arginin ved aminosyreposition 258 er tyrosin, leucin ved aminosyreposition 262 er glutamin, leucin ved aminosyreposition 262 er histidin, leucin ved aminosyreposition 262 er methionin, leucin ved aminosyreposition 262 er prolin, serin ved aminosyreposition 270 er arginin, phenylalanin ved aminosyreposition 271 eralanin, glutaminsyre ved aminosyreposition 277 er serin, arginin ved aminosyreposition 280 erglycin, serin ved aminosyreposition 290 er alanin, threonin ved aminosyreposition 297 eralanin, threonin ved aminosyreposition 297 er prolin, leucin ved aminosyreposition 298 er alanin, leucin ved aminosyreposition 298 er arginin, leucin ved aminosyreposition 298 er asparagin, leucin ved aminosyreposition 298 er serin, leucin ved aminosyreposition 298 ervalin, lysin ved aminosyreposition 300 er glycin, threonin ved aminosyreposition 301 er glutamin, asparaginsyre ved aminosyreposition 305 er prolin, glycin ved aminosyreposition 312 er isoleucin, og/eller serin ved aminosyreposition 315 er isoleucin.
10. Isoleret, syntetisk eller rekombinant polypeptid ifølge krav 8, hvor amino- syresekvensen omfatter en sekvensmodifikation af SEQ ID NO: 2 og (a) modifikationen omfatter eller består af følgende ændringer: phenylalanin ved aminosyreposition 38 er tyrosin, tyrosin ved aminosyreposition 61 er glutamin, methionin ved aminosyreposition 69 er glutaminsyre, isoleucin ved aminosyreposition 94 er glutamin, serin ved aminosyreposition 183 er arginin, serin ved aminosyreposition 191 er alanin, og methionin ved aminosyreposition 276 er alanin, (b) modifikationen omfatter eller består af følgende ændringer: phenylalanin ved aminosyreposition 38 er tyrosin, tyrosin ved aminosyreposition 61 erglutamin, methionin ved aminosyreposition 69 er glutaminsyre, eroleucin ved aminosyreposition 94 erglutamin, eroleucin ved aminosyreposition 166 ervalin, serin ved aminosyreposition 183 er arginin, serin ved aminosyreposition 191 er alanin, glutaminsyre ved aminosyreposition 212 er prolin, methionin ved aminosyreposition 276 er alanin, og arginin ved aminosyreposition 280 erglycin, (c) modifikationen omfatter eller består af følgende ændringer: phenylalanin ved aminosyreposition 38 er tyrosin, tyrosin ved aminosyreposition 61 erglutamin, methionin ved aminosyreposition 69 er glutaminsyre, isoleucin ved aminosyreposition 94 erglutamin, isoleucin ved aminosyreposition 166 ervalin, serin ved aminosyreposition 183 er arginin, serin ved aminosyreposition 191 er alanin, glutaminsyre ved aminosyreposition 212 er prolin, methionin ved aminosyreposition 276 er alanin, arginin ved aminosyreposition 280 erglycin, og threonin ved aminosyreposition 297 er prolin, (d) modifikationen omfatter eller består af følgende ændringer: tyrosin ved aminosyreposition 61 erglutamin, methionin ved aminosyreposition 69 er glutaminsyre, isoleucin ved aminosyreposition 94 erglutamin, isoleucin ved aminosyreposition 166 ervalin, serin ved aminosyreposition 183 er arginin, serin ved aminosyreposition 191 er alanin, glutaminsyre ved aminosyreposition 212 er prolin, methionin ved aminosyreposition 276 er alanin, arginin ved aminosyreposition 280 erglycin, threonin ved aminosyreposition 297 er prolin, og threonin ved aminosyreposition 301 erglutamin, (e) modifikationen omfatter eller består af følgende ændringer: tyrosin ved aminosyreposition 61 erglutamin, methionin ved aminosyreposition 69 er glutaminsyre, arginin ved aminosyreposition 71 er serin, isoleucin ved aminosyreposition 94 erglutamin, isoleucin ved aminosyreposition 166 ervalin, serin ved aminosyreposition 183 er arginin, serin ved aminosyreposition 191 er alanin, glutaminsyre ved aminosyreposition 212 er prolin, methionin ved aminosyreposition 276 er alanin, arginin ved aminosyreposition 280 erglycin, threonin ved aminosyreposition 297 er prolin, og threonin ved aminosyreposition 301 erglutamin, (f) modifikationen omfatter eller består af følgende ændringer: tyrosin ved aminosyreposition 61 erglutamin, methionin ved aminosyreposition 69 er glutaminsyre, asparaginsyre ved aminosyreposition 70 er prolin, arginin ved aminosyreposition 71 er serin, isoleucin ved aminosyreposition 166 ervalin, serin ved aminosyreposition 183 er arginin, serin ved aminosyreposition 191 er alanin, glutaminsyre ved aminosyreposition 212 er prolin, methionin ved aminosyreposition 276 er alanin, arginin ved aminosyreposition 280 erglycin, threonin ved aminosyreposition 297 er prolin, og threonin ved aminosyreposition 301 erglutamin, eller (g) modifikationen omfatter eller består af følgende ændringer: phenylalanin ved aminosyreposition 38 er tyrosin, tyrosin ved aminosyreposition 61 erglutamin, methionin ved aminosyreposition 69 er glutaminsyre, arginin ved aminosyreposition 71 er serin, isoleucin ved aminosyreposition 166 ervalin, serin ved aminosyreposition 183 er arginin, serin ved aminosyreposition 191 eralanin, glutaminsyre ved aminosyreposition 212 er prolin, leucin ved aminosyreposition 231 ervalin, methionin ved aminosyreposition 276 er alanin, arginin ved aminosyreposition 280 erglycin, threonin ved aminosyreposition 297 er prolin, og threonin ved aminosyreposition 301 erglutamin.
11. Fremgangsmåde til fremstilling af et rekombinant polypeptid omfattende (i) (a) tilvejebringelse af nucleinsyren ifølge et vilkårligt af kravene 1 til 4, og (b) udtrykkelse af nucleinsyren fra trin (a) ved betingelser, som tillader ekspression af polypeptidet, og dermed fremstille et rekombinant polypeptid ifølge krav 8, eller (ii) fremgangsmåden ifølge krav (i), som yderligere omfatter at transformere en værtscelle med nucleinsyren fra trin (i) (a) efterfulgt af fremstilling af nucleinsyren fra trin (a), og dermed fremstille et rekombinant polypeptid ifølge krav 8 i en transformeret celle.
12. Fremgangsmåde til generering af en variant af en nucleinsyre, som koder for et polypeptid med en glucanaseaktivitet omfattende: (i) (a) tilvejebringelse af en templatnucleinsyre omfattende nucleinsyresekven-sen ifølge et vilkårligt af kravene 1 til 4, og (b) modificering, deletering eller addering af en eller flere nucleotider i tem-platsekvensen eller en kombination heraf til at generere en variant af templat-nucleinsyren, hvor fremgangsmåden eventuelt yderligere omfatter at udtrykke variantnuclein-syren for at generere et variantglucanasepolypeptid, (ii) fremgangsmåde ifølge (i), hvor modifikationerne, additionerne eller delete-ringerne er indført med en fremgangsmåde omfattende error-prone PCR, blanding, oligonucleotidrettet mutagenese, samlings PCR, seksuel PCR mutagene-se, mutagenese in vivo, kassettemutagene4se, samlingsmutagenese, rekursiv samlingsmutagenese, eksponentiel samlingsmutagenese, stedsspecifik mutagenese, gengensamling, Gene Site Saturation Mutagenesis (GSSM), syntetisk ligeringsgensamling (SLR), rekombination, rekursiv sekvensrekombination, phosphotioat modificeret DNA-mutagenese, uracilholdig templatmutagenese, gapped dupleks mutagenese, punktfejlparringsreparationsmutagenese, repara-tionsdefficient værtsstammemutagenese, kemisk mutagenese, radiogen mutagenese, deleterings mutagenese, restriktionsselektionsmutagenese, restrik-tionsoprensningsmutagenese, kunstig gensyntese, kimærisk nucleinsyremulti-merskabelse eller en kombination heraf, (iii) fremgangsmåde ifølge (i) eller (ii), hvor fremgangsmåden iterativt gentages indtil et variantglucanasepolypeptid med ændret eller forskellig aktivitet eller ændret eller forskellig stabilitet i forhold til et polypeptid kodet for af templatnu-cleinsyren, fremstilles, (iv) fremgangsmåde ifølge krav (iii), hvor variantglucanasepolypeptidet er ter-motolerant og bevarer nogen aktivitet efter at være udsat for en forhøjet temperatur eller variantglucanasepolypeptidet har forøget glycosylering sammenlignet med glucanasen kodet af en templatnucleinsyre, eller variantglucanasepolypeptidet har en glucanaseaktivitet ved høj temperatur, hvor glucanasen kodet af templatnucleinsyren ikke er aktiv ved den høje temperatur, (v) fremgangsmåde ifølge (iii), hvor fremgangsmåden iterativt gentages indtil en glucanasekodende sekvens har en ændret codonanvendelse i forhold til templatnucleinsyren er fremstillet, eller (vi) fremgangsmåde ifølge (iii), hvor fremgangsmåden iterativt gentages indtil et glucanasegen med højere eller lavere messageproduktion eller stabilitet, i forhold til templatnucleinsyren er fremstillet.
13. Fremgangsmåde til at hydrolysere, brække eller afbryde en glucan-, xy-lan- og/eller mannanomfattende sammensætning omfattende følgende trin: (a) tilvejebringelse af polypeptidet med glucanase-, xylanase- og/eller manna-naseaktivitet ifølge et vilkårligt af kravene 8 til 10, eller et polypeptid kodet for af nucleinsyresekvensen ifølge et vilkårligt af kravene 1 til 4, (b) tilvejebringelse af en sammensætning omfattende en glucan, xylan og/eller mannan, og (c) bringe polypeptidet fra trin (a) i kontakt med sammensætningen fra trin (b) ved betingelser, hvor glucanase, xylanase og/eller mannannase hydrolyserer, brækker eller afbryder den glucan-, xylan- eller mannanholdige sammensætning, hvor eventuelt sammensætningen omfatter en plantecelle, en bakteriecelle, en gærcelle, en insektcelle, en fungal celle eller en animalsk celle.
14. Fremgangsmåde til fremstilling af et brændstof omfattende at bringe en biomasse i kontakt med polypeptidet kodet for af nucleinsyren ifølge et vilkårligt af kravene 1 til 4 eller polypeptidet ifølge et vilkårligt af kravene 8 til 10, hvor fremgangsmåden eventuelt omfatter: (A) at bringe en sammensætning omfattende celloligosaccharid, en arabinoxy-lanoligomer, en lignin, en lignocellulose, en xylan, en glucan, en cellulose eller en fermenteringsdygtig sukker i kontakt med polypeptidet kodet for af nucleinsyren ifølge et vilkårligt af kravene 1 til 4 eller polypeptidet ifølge et vilkårligt af kravene 8 til 10, (B) fremgangsmåde ifølge (A), hvor sammensætningen omfattende cellooligo-saccharidet, arabinoxylanoligomeren, lignin, lignocellulose, xylan, glycan, cellulose eller fermenteringsdygtigt sukker omfatter en plante, planteprodukt eller plantederivat, (C) fremgangsmåde ifølge (B), hvor planten eller planteproduktet omfatter rørsukkerplanter eller planteprodukter, roer eller sukkerroer, hvede, majs, sojabønner, kartofler, ris eller byg, (D) fremgangsmåde ifølge (B), hvor planten er en enkimbladet eller tokimbladet plante, eller planten er enkimbladet majs, sukkerrør, ris, hvede, byg, switch-grass eller Miscanthus, eller planten er en tokimbladet oliefrøsafgrøde, soja, canola, raps, hør, bomuld, palmeolie, sukkerroe, jordnød, træ, popler eller lupin, (E) fremgangsmåde ifølge (A), (B), (C) eller (D), hvor polypeptidet har aktivitet omfattende cellulase, endoglucanase, cellobiohydrolase, beta-glucosidase, xy-lanase, mannase, β-xylosidase og/eller arabinofuranosidaseaktivitet, hvor sammensætningen eventuelt yderligere omfatter en glucoseoxidase, en glu-coseoxidase-1 (en β-glucosidase) eller en glucoseoxidase-2 (en β-xylosidase), (F) fremgangsmåde ifølge (A), (B), (C), (D) eller (E), hvor brændstoffet omfatter en væske og/eller en gas, eller brændstoffet omfatter et biobrændset og/eller et syntetisk brændstof, eller brændstoffet omfattet bioethanol, biomethanol, bio-propanol og/eller bio-butanol, og/eller en benzin-ethanol-, -methanol-, -butanol-og/eller -propanolblanding.
15. Boring eller olie- og gasbrøndsvask og/eller fraktureringsfremgangsmåde omfattende (I) (i) tilvejebringelse af en blanding af polymernedbrydende (’’polymer breaking”) enzymer omfattende et polypeptid kodet for af nucleinsyresekvensen ifølge et vilkårligt af kravene 1 til 4 eller et polypeptid ifølge et vilkårligt af kravene 8 til 10, hvor mindst et af enzymerne er et polymernedbrydende (’’polymer breaking”) enzym og eventuelt er det polymernedbrydende (’’polymer breaking”) et ligninnedbrydende enzym, en ligninperoxidase, en polysaccahridnedbrydende (’’polymer breaking”) enzym, et proteinnedbrydende enzym, en amylase, en xanthanase, en glucanase, en protease, en glycosidase og/eller en cellulase, og (ii) tilsætning af den polymernedbrydende (’’polymer breaking” blanding af enzymer til en borevæske, som anvendes under en olie- og gasbrøndsboreopera-tion, eller tilsætning af blandingen af enzymer til en borevæske, som anvendes til olie- og gasbrøndsvask og/eller fraktureringsoperation, eller (II) fremgangsmåde (I) hvor enzymet eventuelt sætte til en væske, hvor væsken eventuelt omfatter stivelse som en viskositetsdanner, hvor enzymet eventuelt er formuleret ved relativ høj alkalinitet, hvor den relativt høje alkalinitet er fra pH 9 til pH 1,5, hvor enzymet eventuelt er et surt-til-neutralt enzym, hvor enzymet eventuelt forbliver sovende i borevæsken eller olie- og gasbrønd-vaskevæsken og/eller fraktureringsvæsken, og i mudderkagen (’’filter kage”), som dannes efter tab afvand fra væsken på dannelsesoverfladen, hvor eventuelt for at aktivere enzymet mudderkagen kan vaskes med en syreopløsning, hvor syren vil neutralisere alkaliniteten af mudderkagen (’’filter kage”) og vil tilvejebringe sure omgivelser, som vil igangsætte enzymaktiviteten og hydrolysefunktionen over for stivelse eller andre polymerer, hvor enzymet eventuelt udsættes for en ’’syrevask”, hvor syrevaskevæsken eventuelt tilføres under brøndboringsoperationer og/eller brøndrengøringsoperationer for at fjerne calciumcarbonataflejringer fra dannelsen, eller hvor enzymet eventuelt, når først det er aktiveret med sure omgivelser, vil nedbryde stivelsen eller andre polymerer og vil fjerne mudderkagen (’’filter kage”) fra brøndboringshullet.
16. Fremgangsmåde til at nedbryde et xanthan-, guar-, hydroxyalkylguar-, car-boxyalkylguar-, guargummi-, guargummipulver-, lignificeret overtræk af guarfrø eller størknet guargummi omfattende (I) (i) tilvejebringelse af mindst et eller en blanding af polymernedbrydende enzymer (’’polymer breaking”) omfattende et polypeptid kodet for af nucleinsyrese-kvensen ifølge et vilkårligt af kravene 1 til 4 eller et polypeptid ifølge et vilkårligt af kravene 8 til 10, hvor mindst et af enzymerne er et polymernedbrydende enzym (’’polymer breaking”), og det polymernedbrydende enzym (’’polymer breaking”) er eventuelt et ligninnedbrydende enzym, en ligninperoxidase, et po-lysaccharidnedbrydende enzym, et proteinnedbrydende enzym, en amylase, en xanthanase, en glucanase, en protease, en glycosidase og/eller en cellulase, og (ii) tilsætning af det polymernedbrydende (’’polymer breaking”) enzym eller blandingen af enzymer til guar, hydroxyalkylguar, carboxyalkylguar, guargummi, guargummipulver, lignificeret overtræk af guarfrø eller størknet guargummi i en mængde tilstrækkelig til at nedbryde guar, hydroxyalkylguar, carboxyalkylguar, guargummi, guargummipulver, lignificeret ovetræk af guarfrø eller størknet guargummi, eller (II) fremgangsmåde ifølge (I), hvor enzymet eventuelt sættes til som en væske, hvor væsken eventuelt omfatter stivelse som en viskositetsdanner, hvor enzymet eventuelt er formuleret ved relativ høj alkalinitet, hvor den relativt høje alkalinitet er pH 9 til pH 9,5, hvor enzymet eventuelt er et surt-til-neutralt enzym, hvor enzymet eventuelt forbliver sovende i borevæsken eller olie- og gasbrønd-vaskevæsken og/eller fraktureringsvæsken og i mudderkagen ("filter kage"), som dannes efter tab af vand fra væsken til dannelsesoverfladen, hvor mudderkagen eventuelt for at aktivere enzymet kan vaskes med en syreopløsning, hvor syren vil neutralisere alkaliniteten af mudderkagen ("filter kage”) og vil tilvejebringe sure omgivelser, som vil igangsætte enzymaktiviteten og den hydrolytiske funktion over for stivelse eller andre polymerer, hvor enzymet eventuelt udsættes for en ’’syrevask”, hvor syrevaskevæsken eventuelt tilføres under brøndboringsoperationer og/eller brøndrengøringsoperationer for at fjerne calciumcarbonataflejringer fra dannelsen, hvor enzymet eventuelt, når først det er aktiveret ved sure betingelser, vil nedbryde stivelsen eller andre polymerer og vil fjerne mudderkagen (’’filter kagen”) fra brøndborehullet, eller hvor blandingen af enzymer eventuelt omfatter mindst et, to, tre, fire, fem, seks, syv, otte, ni eller ti eller alle enzymerne valgt fra gruppen bestående af et lignin-nedbrydende enzym, alpha-amylase, beta-amylase, glucoamylase, dextrinase, cellulase, cellobiohydrolase, avicelase, carboxymethylcellulase, beta-glucana-se, glucosidase, xylanase, mannanase, arabinofuranosidase, laccase, lignin-peroxidase, pectinase, pectate lyase, xanthanase, xanthan-lyase, xanthan de-polymerase, pullulanase, lichenase, pachymanase, lipase, protease, proteinase, phytase, peptidase og catalase, eller amylaser, xanthanaser, glycosidaser, cellulaser) anvendelse af enhver kombination af andre enzymer såsom tryptil-phanaser eller tyrosine-decarboxylaser, laccaser, catalaser, laccaser, andre cellulaser, endoglycosidaser, endo-beta-1,4-laccaser, amyloglucosidaser, andre glucosidaser, glucose-eromeraser, glycosyltransferaser, lipaser, phospholipa-ser, lipooxygenaser, beta-laccaser, endo-beta-1,3(4)-laccaser, cutinaser, per-oxidaser, andre amylaser, xanthanaser, glucoamylaser, pectinaser, reductaser, oxidaser, decarboxylaser, phenoloxidaser, ligninaser, pullulanaser, arabinana- ser, hemicellulaser, mannanaser, xylolaccaser, xylanaser, pectin-acetyl-este-raser, rhamnogalacturonan-acetyl-esteraser, proteaser, peptidaser, proteinaser, polygalacturonaser, rhamnogalacturonaser, galactanaser, pectin-lyaser, trans-glutaminaser, pectin-methylesteraser, andre cellobiohydrolaser og/eller trans-glutaminase-enzymer.
17. Fremgangsmåde til at modificere eller justere de rheologiske egenskaber af: et polysaccharidfortykningsmiddel, et polysaccaridfortykningsmiddel i en gel, et flokkulat, et bindemiddel eller et smøremiddel, eller et polysaccharid i en film for at modificere en egenskab ved filmen, hvilken fremgangsmåde omfatter (I) (i) tilvejebringelse af mindst et eller en blanding af enzymer omfattende: (a) et polypeptid kodet for af nucleinsyresekvensen ifølge et vilkårligt af kravene 1 til 4 eller polypeptidet ifølge et vilkårligt af kravene 8 til 10, eller (b) et polypeptid med en aminosyresekvens fra (a) og bevarende enzymaktivitet og omfattende mindst en aminosyregruppekonservativ substitution, hvor den konservative substitution eventuelt omfatter erstatning af en alifatisk aminosyre med en anden alifatisk aminosyre, erstatning af en serin med threo-nin eller omvendt, erstatning af en sur gruppe med en anden sur gruppe, erstatning af en gruppe med en amidgruppe med en anden gruppe, som har en amidgruppe, udveksling af en basisk gruppe med en anden basisk gruppe, eller erstatning af en aromatisk gruppe med en anden aromatisk gruppe eller en kombination heraf, og eventuelt omfatter de alifatiske grupper alanin, valin, leucin, isoleucin eller en syntetisk ækvivalent heraf, de sure grupper omfatter asparaginsyre, glutaminsy-re eller en syntetisk ækvivalent heraf, gruppen omfattende en amidgruppe omfatter asparaginsyre, glutaminsyre eller en syntetisk ækvivalent heraf, den basiske gruppe omfatter lysin, arginin eller en syntetisk ækvivalent heraf, eller den aromatiske gruppe omfatter phenylalanin, tyrosin eller en syntetisk ækvivalent heraf, og (ii) tilsætning af blandingen af enzymer eller enzym til polysaccharidfortyk-ningsmidler, polysaccharidfortykningsmidler i en gel, et flokkulat, et bindemiddel eller et smøremiddel, eller polysaccharidfilmen og derved justere eller modifice- re egenskaberne af gelen, flokkulatet, bindemidlet, smøremidlet eller filmen, eller (II) fremgangsmåde ifølge (I), hvor blandingen af enzymer omfatter mindst en, to, tre, fire, fem, seks, syv, otte, ni eller ti eller alle enzymerne valgt fra gruppen bestående af ligninnedbrydende enzym, alpha-amylase, beta-amylase, gluco-amylase, dextrinase, cellulase, cellobiohydrolase, avicelase, carboxymethylcel-lulase, beta-glucanase, glucosidase, xylanase, mannanase, arabinofuranosi-dase, laccase, lignin-peroxidase, pectinase, pectat-lyase, xanthanase, xanthan-lyase, xanthan-depolymerase, pullulanase, lichenase, pachymanase, lipase, protease, proteinase, phytase, peptidase og catalase, eller amylaser, xanthana-ser, glycosidaser, cellulaser) anvendelse af en enhver kombination af eller enzymer, såsom tryptophanaser eller tyrosin-decarboxylaser, laccaser, catalaser, laccaser, andre cellulaser, endoglycosidaser, endo-beta-1,4-laccaser, amyloglu-cosidaser, andre glucosidaser, glucose-isomeraser, glycosyltransferaser, lipa-ser, phospholipaser, lipooxygenaser, beta-laccaser, endo-beta-1,3(4)-laccaser, cutinaser, peroxidaser, andre amylaser, xanthanaser, glucoamylaser, pectina-ser, reductaser, oxidaser, decarboxylaser, phenoloxidaser, ligninaser, pullula-naser, arabinanaser, hemicellulaser, mannanaser, xylolaccaser, xylanaser, pec-tin-acetyl-esteraser, rhamnogalacturonan-acetyl-esteraser, proteaser, peptidaser, proteinaser, polygalacturonaser, rhamnogalacturonaser, galactanaser, pec-tin-lyaser, transglutaminaser, pectin-methylesteraser, andre cellobiohydrolaser og/eller transglutaminase-enzymer.
18. Sammensætning omfattende et polypeptid ifølge et vilkårligt af kravene 8 til 10 eller kodet for af nucleinsyren ifølge et vilkårligt af kravene 1 til 4, hvor sammensætningen eventuelt er en farmaceutisk sammensætning, en detergentsammensætning, en kontaktlinseopløsning, en affaldsbehandlingssammensætning, en sæbe eller et tyggegummi, sugetablet eller bolsje, eller hvor eventuelt sammensætningen er et brændstof, hvor eventuelt sammensætningen er en alkohol, hvor alkoholen eventuelt er ethanol, eller hvor sammensætningen eventuelt er et biomassemateriale, papir, papiraffald, genanvendt papirprodukt, papirpulp, papirprodukt, træ, træprodukt, træpulp, kraftpulp, tekstil, vævet stof, tråd, fibre eller et klæde, eller hvor eventuelt biomassematerialet stammer fra en landbrugsmæssig afgrøde, er et biprodukt af en fødevare eller foderproduktion, er et lignocellulose-affaldsprodukt eller er en planteremanens eller affaldspapir eller affaldspapirprodukt og eventuelt omfatter planteremanensen stængler, blade, skaller, avner, majs eller majskolber, majsstilke, hø, strå, træ, stæsmuld, træpulp, træaffald og savsmuld, og eventuelt omfatter papiraffaldet bortkastet eller brugt fotokopieringspapir, computerprinterpapir, notesbogspapir, notesblokpapir, maskinskrivningspapir, aviser, magasiner, pap- og papirbaserede emballagematerialer, eller hvor sammensætningen eventuelt omfatter en lignin, lignocellulose, cellulose, hemicellulose, lignin, xylan, glucan eller mannan, eller hvor sammensætningen eventuelt er en drikkevare, fødevare, fødevareprodukt, foder, foderadditiv, ernæringsmæssigt supplement eller et diætmæssigt supplement eller en spiselig enzymtilførselsmatrix.
19. Fremgangsmåde til behandling eller modificering af en sammensætning omfattende at bringe en sammensætning i kontakt med polypeptidet ifølge et vilkårligt af kravene 8 til 10, eller et polypeptid kodet for af nucleinsyren ifølge et vilkårligt af kravene 1 til 4, hvor sammensætningen eventuelt er en farmaceutisk sammensætning, en detergentsammensætning, en kontaktlinseopløsning, en affaldsbehandlingssammensætning eller en sæbe, hvor sammensætningen eventuelt er et brændstof, hvor sammensætningen eventuelt er en alkohol, hvor alkoholen eventuelt er ethanol, eller hvor sammensætningen eventuelt er et biomassemateriale, papir, papiraffald, genanvendt papirprodukt, papirpulp, papirprodukt, træ, træprodukt, træpulp, kraftpulp, tekstil, stof, tråd, fibre eller et klæde, eller hvor biomassematerialet eventuelt stammer fra en landbrugsmæssig afgrøde, er et biprodukt fra en fødevare- eller en foderproduktion, er et lignocellu-loseaffaldsprodukt, eller en planteremanens eller et affaldspapir eller affaldspapirprodukt, og planteremanensen eventuelt omfatter stængler, blade, skaller, avner, majs eller majskolber, majsstilke, hø, strå, træ, træflies, træpulp, træaffald og savsmuld, og papiraffaldet omfatter eventuelt bortkastet eller brugt foto kopieringspapir, computerprinterpapir, notesbogspapir, notesblokpapir, maskin-skrivningspapir, aviser, magasiner, pap- og papirbaserede emballagematerialer, eller hvor sammensætningen eventuelt omfatter en lignin, lignocellulose, cellulose, hemicellulose, lignin, xylan, glucan eller mannan, eller hvor sammensætningen eventuelt er en drikkevare, en fødevare, et foder, et mejeriprodukt, et ernæringsmæssigt supplement, et tyggegummi, en sugetablet eller et bolsje, eller hvor fremgangsmåden eventuelt er til produktion af brændstof, fødevare, foderstof, ernæringsmæssigt supplement eller drikkevareproduktion.
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-
2007
- 2007-08-04 DK DK07875173.2T patent/DK2069389T3/da active
- 2007-08-04 US US12/375,146 patent/US20110117067A1/en not_active Abandoned
- 2007-08-04 BR BRPI0714876-3A patent/BRPI0714876B1/pt not_active IP Right Cessation
- 2007-08-04 CA CA3020590A patent/CA3020590A1/en not_active Abandoned
- 2007-08-04 CN CN2013103224122A patent/CN103397043A/zh active Pending
- 2007-08-04 CA CA2669453A patent/CA2669453C/en active Active
- 2007-08-04 AU AU2007356171A patent/AU2007356171B8/en not_active Ceased
- 2007-08-04 CN CN201610591549.1A patent/CN106222185B/zh not_active Expired - Fee Related
- 2007-08-04 MX MX2009001318A patent/MX369001B/es active IP Right Grant
- 2007-08-04 EP EP07875173.2A patent/EP2069389B1/en not_active Not-in-force
- 2007-08-04 EP EP20120150998 patent/EP2444413A1/en not_active Ceased
- 2007-08-04 CN CNA2007800368717A patent/CN101528766A/zh active Pending
- 2007-08-04 WO PCT/US2007/075226 patent/WO2009020459A2/en not_active Ceased
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2019
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Also Published As
| Publication number | Publication date |
|---|---|
| WO2009020459A2 (en) | 2009-02-12 |
| WO2009020459A3 (en) | 2009-04-23 |
| BRPI0714876B1 (pt) | 2022-04-19 |
| EP2069389A4 (en) | 2009-12-16 |
| MX369001B (es) | 2019-10-24 |
| CN106222185B (zh) | 2021-12-03 |
| US20190376052A1 (en) | 2019-12-12 |
| CA2669453A1 (en) | 2009-02-12 |
| US20140295523A1 (en) | 2014-10-02 |
| AU2007356171B8 (en) | 2014-01-16 |
| BRPI0714876A2 (pt) | 2017-10-24 |
| AU2007356171B2 (en) | 2013-12-19 |
| EP2069389B1 (en) | 2014-10-08 |
| CA2669453C (en) | 2018-11-13 |
| CN106222185A (zh) | 2016-12-14 |
| EP2069389A2 (en) | 2009-06-17 |
| CN101528766A (zh) | 2009-09-09 |
| EP2444413A1 (en) | 2012-04-25 |
| US20110117067A1 (en) | 2011-05-19 |
| AU2007356171A1 (en) | 2009-02-12 |
| AU2007356171A8 (en) | 2014-02-06 |
| MX2009001318A (es) | 2009-10-20 |
| CA3020590A1 (en) | 2009-02-12 |
| US10329549B2 (en) | 2019-06-25 |
| CN103397043A (zh) | 2013-11-20 |
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