DK2190296T3 - Fremgangsmåde - Google Patents
Fremgangsmåde Download PDFInfo
- Publication number
- DK2190296T3 DK2190296T3 DK08827725.6T DK08827725T DK2190296T3 DK 2190296 T3 DK2190296 T3 DK 2190296T3 DK 08827725 T DK08827725 T DK 08827725T DK 2190296 T3 DK2190296 T3 DK 2190296T3
- Authority
- DK
- Denmark
- Prior art keywords
- seq
- milk
- amino acid
- lipid acyltransferase
- enzyme
- Prior art date
Links
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
- A23C9/1216—Other enzymes
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- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
- A21D8/00—Methods for preparing or baking dough
- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
- A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
- A23C19/00—Cheese; Cheese preparations; Making thereof
- A23C19/02—Making cheese curd
- A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
- A23C19/0328—Enzymes other than milk clotting enzymes, e.g. lipase, beta-galactosidase
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
- A23C19/00—Cheese; Cheese preparations; Making thereof
- A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
- A23C19/068—Particular types of cheese
- A23C19/0684—Soft uncured Italian cheeses, e.g. Mozarella, Ricotta, Pasta filata cheese; Other similar stretched cheeses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L15/00—Egg products; Preparation or treatment thereof
- A23L15/25—Addition or treatment with microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Nutrition Science (AREA)
- Enzymes And Modification Thereof (AREA)
- Dairy Products (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- General Preparation And Processing Of Foods (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (14)
1. Fremgangsmåde til fremstilling af UHT-mælk, hvor fremgangsmåden omfatter blanding af en lipid acyltransferase og mælk eller en fraktion deraf ved en temperatur og i en inkubationsperiode, der er effektiv til at sikre, at der er mindst 5 % transferaseaktivitet målt ved molmængden af cholesterolester dannet ved acyloverførsel fra phospholipider eller triacylglycerider i mælk til cholesterol, i forhold til mængden af oprindeligt tilgængeligt cholesterol, beregnet ved anvendelse af den følgende ligning: Transferaseaktivitet = (Mol/1 cholesterolester (ti - Mol/1 cholesterolester (011 x 100 Mol/1 cholesterol (0) hvor: cholesterolester (t) = mængden af cholesterolester på tidspunktet t cholesterolester (0) = mængden af cholesterolester på tidspunktet 0 cholesterol (0) = mængden af cholesterol i mælk på tidspunktet 0, og behandling af den enzymbehandlede mælk ved ultrahøj varmebehandling for fremstilling af UHT-mælk.
2. Anvendelse af en lipid acyltransferase i fremstillingen af UHT-mælk for mindst én af forbedring af stabiliteten, i særdeleshed langvarig stabilitet, den mærkbare sensoriske forskel eller duften og/eller smagen af UHT-mælken.
3. Anvendelse af en lipid acyltransferase i fremstillingen af UHT-mælk til reducering af cholesterolindholdet i UHT-mælken.
4. Anvendelse af en lipid acyltransferase i fremstillingen af UHT-mælk til eliminering eller reducering af flødeafsætning i UHT-mælken.
5. Fremgangsmåde eller anvendelse ifølge et hvilket som helst af kravene 1-4, hvor den lipide acyltransferase tilsættes til mælken eller en fraktion deraf og inkuberes deri ved en temperatur på mindre end ca. 20 °C, fortrinsvis mindre end ca. 10 °C.
6. Fremgangsmåde eller anvendelse ifølge et hvilket som helst af kravene 1-5, hvor den lipide acyltransferase tilsættes til mælken eller en fraktion deraf og inkuberes deri ved en temperatur på mellem ca. 1 °C og ca. 10 °C, fortrinsvis mellem ca. 3 °C og ca. 7 °C, mere fortrinsvis ca. 5 °C.
7. Fremgangsmåde eller anvendelse ifølge et hvilket som helst af kravene 1-6, hvor den lipide acyltransferase omfatter et GDSx-motiv, hvor x er et eller flere af L, A, V, I, F, Y, H, Q, T, N, M eller S og/eller et GANDY -motiv.
8. Fremgangsmåde eller anvendelse ifølge et hvilket som helst af kravene 1-7, hvor det lipide acyltransferaseenzym omfatter aminosyresekvensmotivet GDSX, hvor X er én eller flere af de følgende aminosyrerester L, A, V, I, F, Y, H, Q, T, N, M eller S.
9. Fremgangsmåde eller anvendelse ifølge et hvilket som helst af kravene 1-8, hvor den lipide acyltransferase kan være opnåelig, fortrinsvis opnået, fra en organisme fra én eller flere af de følgende arter: Aeromonas, Streptomyces, Saccharomyces, Lactococcus, Mycobacterium, Streptococcus, Lactobacillus, Desulfitobacterium, Bacillus, Campylobacter, Vibrionaceae, Xylella, Sulfolobus, Aspergillus, Schizosaccharomyces, Listeria, Neisseria, Mesorhizobium, Ralstonia, Xanthomonas og Candida.
10. Fremgangsmåde eller anvendelse ifølge krav 9, hvor den lipide acyltransferase er opnåelig, fortrinsvis opnås, fra en organisme fra slægten Aeromonas.
11. Fremgangsmåde eller anvendelse ifølge et hvilket som helst af kravene 1-10, hvor den lipide acyltransferase er et polypeptid opnået ved ekspression af en hvilket som helst af nukleotidsekvenseme ifølge SEQ ID NO: 36, SEQ ID NO: 39, SEQ ID NO: 42, SEQ ID NO: 44, SEQ ID NO: 46, SEQ ID NO: 48, SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53, SEQ ID NO: 54, SEQ ID NO: 55, SEQ ID NO: 56, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 60, SEQ ID NO: 61, SEQ ID NO: 62 eller SEQ ID NO: 63 eller en nukleotidsekvens, der er 75 % eller mere identisk dermed.
12. Fremgangsmåde eller anvendelse ifølge et hvilket som helst af kravene 1-11, hvor den lipide acyltranferase er et polypeptid opnået ved ekspression af: a. nukleotidsekvensen ifølge SEQ ID NO: 49 eller en nukleotidsekvens, der er 75 % eller mere identisk dermed; b. en nukleinsyre, der koder for polypeptidet, hvor polypeptidet er mindst 70 % identisk med polypeptidsekvensen ifølge SEQ ID NO: 16 eller med polypeptidsekvensen ifølge SEQ ID NO: 68; eller c. en nukleinsyre, der hybridiseres under medium stringensforhold til en nukleinprobe, der omfatter nukleotidsekvensen ifølge SEQ ID NO: 49.
13. Fremgangsmåde eller anvendelse ifølge et hvilket som helst af kravene 1-12, hvor den lipide acyltransferase er et polypeptid, der omfatter en hvilken som helst af aminosyresekvenseme ifølge SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 68 eller en aminosyresekvens, der er 75 % eller mere identisk dermed.
14. Fremgangsmåde eller anvendelse ifølge et hvilket som helst af kravene 1-13, hvor den lipide acyltranferase er et polypeptid, der omfatter aminosyresekvensen ifølge SEQ ID NO: 68 eller en aminosyresekvens, der er 75 % eller mere identisk dermed. Fremgangsmåde eller anvendelse ifølge et hvilket som helst af kravene 12-14, hvor polypeptidet opnås ved ekspression i Bacillus licheniformis.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0716126.8A GB0716126D0 (en) | 2007-08-17 | 2007-08-17 | Process |
| PCT/IB2008/002573 WO2009024862A2 (en) | 2007-08-17 | 2008-08-14 | Process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2190296T3 true DK2190296T3 (da) | 2014-12-01 |
Family
ID=38566602
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK08709551.9T DK2190864T3 (da) | 2007-08-17 | 2008-02-27 | Lipid-acyltransferase |
| DK08827725.6T DK2190296T3 (da) | 2007-08-17 | 2008-08-14 | Fremgangsmåde |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK08709551.9T DK2190864T3 (da) | 2007-08-17 | 2008-02-27 | Lipid-acyltransferase |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US8652809B2 (da) |
| EP (2) | EP2190864B1 (da) |
| CN (3) | CN101874039A (da) |
| AR (1) | AR067953A1 (da) |
| AU (2) | AU2008290424B2 (da) |
| BR (2) | BRPI0814971A8 (da) |
| CA (2) | CA2695562C (da) |
| CL (1) | CL2008002415A1 (da) |
| DK (2) | DK2190864T3 (da) |
| EA (2) | EA018153B1 (da) |
| ES (2) | ES2415873T3 (da) |
| GB (1) | GB0716126D0 (da) |
| MX (2) | MX2010001856A (da) |
| NZ (2) | NZ583047A (da) |
| PL (2) | PL2190864T3 (da) |
| SA (1) | SA08290513B1 (da) |
| WO (2) | WO2009024736A1 (da) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR0209154A (pt) | 2001-05-18 | 2004-07-20 | Danisco | Processo de preparação de uma massa com uma enzima |
| CN101052702B (zh) | 2004-07-16 | 2013-01-09 | 杜邦营养生物科学有限公司 | 脂肪分解酶及其在食品工业中的应用 |
| MX2010007014A (es) * | 2007-12-21 | 2010-09-30 | Danisco | Procedimiento para refinacion de aceite comestible utilizando una lipido aciltransferasa. |
| GB0905367D0 (en) * | 2009-03-27 | 2009-05-13 | Danisco | Method |
| CN102428177B (zh) | 2009-05-19 | 2015-01-14 | 杜邦营养生物科学有限公司 | 提高面包耐码垛性的方法及其产品 |
| KR101738369B1 (ko) | 2009-07-22 | 2017-05-22 | 그뤼넨탈 게엠베하 | 핫 멜트 압출된 제어 방출 투여형 |
| GB0920089D0 (en) * | 2009-11-17 | 2009-12-30 | Danisco | Method |
| CN102844418B (zh) | 2010-03-12 | 2015-11-25 | 杜邦营养生物科学有限公司 | 方法 |
| GB201007668D0 (en) * | 2010-05-07 | 2010-06-23 | Danisco | Method |
| BR112012032176A2 (pt) | 2010-06-17 | 2015-09-08 | Dupont Nutrition Biosci Aps | processo de tratamento de sementes que contém óleo, método de obtenção de óleo de sementes vegetais, processo de produção de um óleo vegetal refinado e óleo vegetal bruto ou refinado que pode ser obtido por meio de um método ou processo |
| WO2012114232A1 (en) | 2011-02-23 | 2012-08-30 | Dupont Nutrition Biosciences Aps | Process |
| CA3004088A1 (en) | 2011-02-23 | 2012-08-30 | Dupont Nutrition Biosciences Aps | Enzymatic treatment of plant oil |
| WO2013104659A2 (en) | 2012-01-13 | 2013-07-18 | Dupont Nutrition Biosciences Aps | Process |
| WO2013104660A1 (en) | 2012-01-13 | 2013-07-18 | Dupont Nutrition Biosciences Aps | Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation |
| WO2013160372A1 (en) | 2012-04-27 | 2013-10-31 | Dupont Nutrition Biosciences Aps | Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme |
| US20170121741A1 (en) | 2014-04-01 | 2017-05-04 | Dupont Nutrition Biosciences Aps | Method for increasing crude palm oil yields |
| MY190749A (en) * | 2016-08-30 | 2022-05-12 | Mitsubishi Chem Corp | Method for producing mutant enzyme, and mutant alcohol acyltransferase |
| CN113545393A (zh) * | 2020-04-23 | 2021-10-26 | 内蒙古伊利实业集团股份有限公司 | 一种减少杀菌机结垢的方法、液体乳制品及其制备方法 |
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| DK1620551T3 (da) | 2003-04-28 | 2014-01-13 | Chr Hansen As | Phospholipase og fremgangsmåde til fremstilling deraf |
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| GB0405637D0 (en) | 2004-03-12 | 2004-04-21 | Danisco | Protein |
| CN101052702B (zh) * | 2004-07-16 | 2013-01-09 | 杜邦营养生物科学有限公司 | 脂肪分解酶及其在食品工业中的应用 |
| ATE342951T1 (de) | 2004-08-06 | 2006-11-15 | Smet Engineering N V De | Verfahren zum rückgewinnen von öl |
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| EP2298872A3 (en) * | 2004-09-30 | 2011-08-10 | Novozymes A/S | Polypeptides having lipase activity and polynucleotides encoding same |
| DE102005039836A1 (de) * | 2005-08-23 | 2007-03-01 | Cognis Ip Management Gmbh | Sterolesterpulver |
| EP1788080A1 (en) | 2005-11-22 | 2007-05-23 | Süd-Chemie Ag | Use of a thermostable phospholipase in the degumming of an oil or fat, and a method for obtaining a thermostable phopholipase |
| CN101200754B (zh) | 2007-12-07 | 2010-06-02 | 中国农业科学院油料作物研究所 | 无溶剂系统中固定化全细胞酶催化生产植物甾醇酯的方法 |
| JP5211852B2 (ja) | 2008-05-23 | 2013-06-12 | 株式会社Ｉｈｉ | 加圧浮上装置及び加圧浮上方法 |
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