DK2405007T3 - Fremstilling af en lipid acyltransferase ud af transformerede Bacillus licheniformis-celler - Google Patents

Fremstilling af en lipid acyltransferase ud af transformerede Bacillus licheniformis-celler Download PDF

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DK2405007T3
DK2405007T3 DK11184428.8T DK11184428T DK2405007T3 DK 2405007 T3 DK2405007 T3 DK 2405007T3 DK 11184428 T DK11184428 T DK 11184428T DK 2405007 T3 DK2405007 T3 DK 2405007T3
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DK11184428.8T
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DK2405007T5 (da
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Marc Kolkman
Joern Dalgaard Mikkelsen
Rikke Hoegh Lorentsen
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Dupont Nutrition Biosci Aps
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (6)

1. Fremgangsmåde til fremstilling af en lipid acyltransferase, hvilken fremgangsmåde omfatter trinnene: (i) tilvejebringelse afen Bacillus-ceWe, hvor Bacillus-ceWen er B. licheniformis; (ii) omdannelse af Bacillus-ceWen, hvor Bacillus-ceWen er B. licheniformis, med den heterolog nukleotidsekvens, der koder for en lipid acyltransferase ifølge SEQ ID NO: 49; og (iii) ekspression af den lipide acyltransferase i cellen under styring af en promotersekvens.
2. Fremgangsmåde ifølge krav 1, hvor promotersekvensen ikke er nativt forbundet med den nukleotidsekvens, der koder for en lipid acyltransferase.
3. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor fremgangsmåden omfatter det supplerende trin med isolering/genvinding af den lipide acyltransferase.
4. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor promotersekvensen er homolog med værtscellen.
5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor promotersekvensen er udvalgt fra gruppen bestående af en α-amylase-promotersekvens, en protease-promotersekvens, en subtilisin-promotersekvens, en glutaminsyrespecifik protease-promotersekvens og en levansucrase-promotersekvens.
6. Lipid acyltransferase, der kan opnås ved hjælp af fremgangsmåden ifølge et hvilket som helst af de foregående krav, og som kodes for af SEQ ID NO: 49.
DK11184428.8T 2007-01-25 2007-01-25 Fremstilling af en lipid acyltransferase ud af transformerede Bacillus licheniformis-celler DK2405007T5 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11184428.8A EP2405007B1 (en) 2007-01-25 2007-01-25 Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
PCT/IB2007/000558 WO2008090395A1 (en) 2007-01-25 2007-01-25 Production of a lipid acyltransferase from transformed bacillus licheniformis cells
EP07705681A EP2109670A1 (en) 2007-01-25 2007-01-25 Production of a lipid acyltransferase from transformed bacillus licheniformis cells

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DK2405007T3 true DK2405007T3 (da) 2014-02-24
DK2405007T5 DK2405007T5 (da) 2014-06-23

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US (1) US7960150B2 (da)
EP (2) EP2405007B1 (da)
JP (1) JP5124593B2 (da)
CN (1) CN101652474B (da)
AU (1) AU2007344910B2 (da)
BR (1) BRPI0720801A2 (da)
CA (1) CA2673954C (da)
DK (1) DK2405007T5 (da)
MX (1) MX2009008021A (da)
PL (1) PL2405007T3 (da)
WO (1) WO2008090395A1 (da)

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