DK2638149T3 - Immortalisering af epitelceller og fremgangsmåder til anvendelse - Google Patents
Immortalisering af epitelceller og fremgangsmåder til anvendelse Download PDFInfo
- Publication number
- DK2638149T3 DK2638149T3 DK11839723.1T DK11839723T DK2638149T3 DK 2638149 T3 DK2638149 T3 DK 2638149T3 DK 11839723 T DK11839723 T DK 11839723T DK 2638149 T3 DK2638149 T3 DK 2638149T3
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- DK
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- Prior art keywords
- immortalization
- methods
- epithel
- cells
- epithel cells
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0683—Cells of the male genital tract, e.g. prostate, epididymis; Non-germinal cells from testis, e.g. Leydig cells, Sertoli cells
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0631—Mammary cells
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
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- C12N2501/11—Epidermal growth factor [EGF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
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- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1323—Adult fibroblasts
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- C12N2510/00—Genetically modified cells
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- C12Q2600/00—Oligonucleotides characterized by their use
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- General Engineering & Computer Science (AREA)
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- Dermatology (AREA)
- Medicinal Chemistry (AREA)
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- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41329110P | 2010-11-12 | 2010-11-12 | |
| US201161474901P | 2011-04-13 | 2011-04-13 | |
| PCT/US2011/060378 WO2012065067A2 (en) | 2010-11-12 | 2011-11-11 | Immortalization of epithelial cells and methods of use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2638149T3 true DK2638149T3 (da) | 2019-08-12 |
Family
ID=46051584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11839723.1T DK2638149T3 (da) | 2010-11-12 | 2011-11-11 | Immortalisering af epitelceller og fremgangsmåder til anvendelse |
Country Status (7)
| Country | Link |
|---|---|
| US (5) | US9279106B2 (da) |
| EP (2) | EP2638149B1 (da) |
| JP (2) | JP6076258B2 (da) |
| CA (1) | CA2817712C (da) |
| DK (1) | DK2638149T3 (da) |
| FI (1) | FI3553168T3 (da) |
| WO (1) | WO2012065067A2 (da) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2638149B1 (en) * | 2010-11-12 | 2019-05-15 | Georgetown University | Immortalization of epithelial cells and methods of use |
| WO2014082096A1 (en) * | 2012-11-26 | 2014-05-30 | The Trustees Of Columbia University In The City Of New York | Method for culture of human and mouse prostate organoids and uses thereof |
| CA2895791A1 (en) * | 2013-01-25 | 2014-07-31 | Xcell Biosciences, Inc. | Methods, compositions, kits, and systems for selective enrichment of target cells |
| WO2014172340A1 (en) * | 2013-04-15 | 2014-10-23 | Georgetown University | Immortalization of circulating tumor cells and methods of use |
| WO2015192035A1 (en) * | 2014-06-13 | 2015-12-17 | Georgetown University | Compositions and methods for immortalization of epithelial cells |
| CN107075469A (zh) * | 2014-06-27 | 2017-08-18 | 加利福尼亚大学董事会 | 培养的哺乳动物角膜缘干细胞、其产生方法和其用途 |
| KR102276424B1 (ko) | 2014-10-06 | 2021-07-12 | 삼성전자주식회사 | 로-키나제 저해제를 포함하는 세포의 노화를 감소시키기 위한 조성물 및 그의 용도 |
| WO2016161192A1 (en) | 2015-04-03 | 2016-10-06 | Propagenix Inc. | Ex vivo proliferation of epithelial cells |
| US10100285B2 (en) | 2015-04-03 | 2018-10-16 | Propagenix Inc. | Ex vivo proliferation of epithelial cells |
| WO2017044454A1 (en) | 2015-09-11 | 2017-03-16 | Propagenix Inc. | Ex vivo proliferation of epithelial cells |
| US11753626B2 (en) | 2016-03-09 | 2023-09-12 | Beijing Percans Oncology Co., Ltd. | Tumor cell suspension cultures and related methods |
| WO2017214465A1 (en) * | 2016-06-09 | 2017-12-14 | Georgetown University | Process for continuous cell culture of islet cells |
| CN107267446A (zh) * | 2017-07-27 | 2017-10-20 | 广州齐志生物工程设备有限公司 | 一种应用于条件性重编程技术的饲养细胞的处理方法 |
| CN110093309B (zh) * | 2018-01-29 | 2021-07-02 | 中国科学院动物研究所 | 一种诱导成纤维细胞转分化为脂肪细胞的方法 |
| CN110093310B (zh) * | 2018-01-29 | 2021-07-02 | 中国科学院动物研究所 | 一种将成纤维细胞转化为永生化细胞的方法及其应用 |
| CN113881624A (zh) * | 2018-01-29 | 2022-01-04 | 中国科学院动物研究所 | 一种细胞诱导的方法 |
| US20210147811A1 (en) * | 2018-04-09 | 2021-05-20 | Cedars-Sinai Medical Center | Methods for in vitro expansion of adult tissue stem cells |
| JP7478720B2 (ja) * | 2018-04-13 | 2024-05-07 | シャンハイ エルアイディーイー バイオテック カンパニー リミテッド | 条件付き再プログラム化細胞から動物モデルを得るための方法および抗腫瘍薬のスクリーニングのための動物モデルの使用 |
| CN112126627B (zh) * | 2020-09-30 | 2022-08-19 | 扬州大学 | 一种犬角膜上皮细胞永生化细胞系的构建方法和应用 |
| CN114836383B (zh) * | 2021-02-01 | 2024-06-25 | 合肥中科普瑞昇生物医药科技有限公司 | 肠癌原代细胞的培养基及培养方法 |
| JP7635661B2 (ja) * | 2021-07-09 | 2025-02-26 | 横河電機株式会社 | 判定装置、判定方法、および判定プログラム |
| CN118541472A (zh) * | 2021-11-12 | 2024-08-23 | Ism有限公司 | 大肠上皮细胞的长期培养 |
| WO2023217123A1 (zh) * | 2022-05-10 | 2023-11-16 | 上海赛立维生物科技有限公司 | 肺前体样细胞的制备方法和应用 |
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| US5756122A (en) | 1995-06-07 | 1998-05-26 | Georgetown University | Liposomally encapsulated nucleic acids having high entrapment efficiencies, method of manufacturer and use thereof for transfection of targeted cells |
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| GB0206860D0 (en) | 2002-03-22 | 2002-05-01 | Glaxo Group Ltd | Compounds |
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| AU2003220935A1 (en) | 2002-04-03 | 2003-10-13 | Sumitomo Pharmaceuticals Company, Limited. | Benzamide derivatives |
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| WO2004112719A2 (en) | 2003-06-19 | 2004-12-29 | Smithkline Beecham Corporation | Chemical compounds |
| WO2005003101A2 (en) | 2003-07-02 | 2005-01-13 | Biofocus Discovery Limited | Pyrazine and pyridine derivatives as rho kinase inhibitors |
| US20100016329A1 (en) * | 2008-07-21 | 2010-01-21 | Kaohsiung Medical University | Xanthine-based cyclic gmp-enhancing rho-kinase inhibitor inhibits physiological activities of lung epithelial cell line |
| JP5390624B2 (ja) | 2008-11-04 | 2014-01-15 | バイアサイト インク | 幹細胞集合体懸濁液組成物、その分化方法 |
| US8008075B2 (en) * | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
| KR101774546B1 (ko) | 2008-11-20 | 2017-09-04 | 얀센 바이오테크 인코포레이티드 | 마이크로-캐리어 상의 만능 줄기 세포 배양 |
| WO2010065907A2 (en) * | 2008-12-05 | 2010-06-10 | The United States Of America As Represented By The Secretary, Department Of Health And Human Services | Use of a rock inhibitor to sustain primary human keratinocytes in a proliferative state |
| EP2421957B1 (en) * | 2009-04-22 | 2020-11-18 | Viacyte, Inc. | Cell compositions derived from dedifferentiated reprogrammed cells |
| US20130071927A1 (en) * | 2010-05-05 | 2013-03-21 | Sydney Ivf Limited | Media and methods for cell culture |
| EP2638149B1 (en) * | 2010-11-12 | 2019-05-15 | Georgetown University | Immortalization of epithelial cells and methods of use |
-
2011
- 2011-11-11 EP EP11839723.1A patent/EP2638149B1/en active Active
- 2011-11-11 JP JP2013538933A patent/JP6076258B2/ja active Active
- 2011-11-11 CA CA2817712A patent/CA2817712C/en active Active
- 2011-11-11 WO PCT/US2011/060378 patent/WO2012065067A2/en not_active Ceased
- 2011-11-11 FI FIEP19174241.0T patent/FI3553168T3/fi active
- 2011-11-11 EP EP19174241.0A patent/EP3553168B1/en active Active
- 2011-11-11 DK DK11839723.1T patent/DK2638149T3/da active
- 2011-11-11 US US13/885,078 patent/US9279106B2/en active Active
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2014
- 2014-09-26 US US14/498,089 patent/US9657272B2/en active Active
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2016
- 2016-02-10 US US15/040,770 patent/US10041048B2/en active Active
- 2016-02-10 US US15/040,783 patent/US9951315B2/en active Active
- 2016-11-21 JP JP2016226280A patent/JP2017104097A/ja active Pending
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2018
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Also Published As
| Publication number | Publication date |
|---|---|
| US20160289644A1 (en) | 2016-10-06 |
| EP2638149B1 (en) | 2019-05-15 |
| US9657272B2 (en) | 2017-05-23 |
| US11001808B2 (en) | 2021-05-11 |
| EP3553168A1 (en) | 2019-10-16 |
| WO2012065067A3 (en) | 2013-09-19 |
| CA2817712A1 (en) | 2012-05-18 |
| EP2638149A4 (en) | 2014-09-17 |
| EP2638149A2 (en) | 2013-09-18 |
| FI3553168T3 (fi) | 2026-03-11 |
| US9951315B2 (en) | 2018-04-24 |
| US20190233798A1 (en) | 2019-08-01 |
| JP6076258B2 (ja) | 2017-02-08 |
| US20130309681A1 (en) | 2013-11-21 |
| CA2817712C (en) | 2020-03-24 |
| US20150099267A1 (en) | 2015-04-09 |
| US10041048B2 (en) | 2018-08-07 |
| US9279106B2 (en) | 2016-03-08 |
| US20160289643A1 (en) | 2016-10-06 |
| WO2012065067A2 (en) | 2012-05-18 |
| JP2017104097A (ja) | 2017-06-15 |
| EP3553168B1 (en) | 2025-12-31 |
| JP2014502151A (ja) | 2014-01-30 |
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