DK3489366T3 - Fremgangsmåder og produkter til produktion af manipulerede mammale cellelinier med forstærkede transgener - Google Patents
Fremgangsmåder og produkter til produktion af manipulerede mammale cellelinier med forstærkede transgener Download PDFInfo
- Publication number
- DK3489366T3 DK3489366T3 DK18211367.0T DK18211367T DK3489366T3 DK 3489366 T3 DK3489366 T3 DK 3489366T3 DK 18211367 T DK18211367 T DK 18211367T DK 3489366 T3 DK3489366 T3 DK 3489366T3
- Authority
- DK
- Denmark
- Prior art keywords
- transgenes
- amplified
- products
- methods
- cell lines
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
- C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
- C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
- C12Y105/01003—Dihydrofolate reductase (1.5.1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
- C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
- C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/04—Phosphoric diester hydrolases (3.1.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161492174P | 2011-06-01 | 2011-06-01 | |
| EP12792301.9A EP2714936B1 (en) | 2011-06-01 | 2012-06-01 | Methods and products for producing engineered mammalian cell lines with amplified transgenes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3489366T3 true DK3489366T3 (da) | 2020-02-24 |
Family
ID=47260412
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12792301.9T DK2714936T3 (da) | 2011-06-01 | 2012-06-01 | Fremgangsmåder og produkter til produktion af manipulerede mammale cellelinier med forstærkede transgener |
| DK18211367.0T DK3489366T3 (da) | 2011-06-01 | 2012-06-01 | Fremgangsmåder og produkter til produktion af manipulerede mammale cellelinier med forstærkede transgener |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12792301.9T DK2714936T3 (da) | 2011-06-01 | 2012-06-01 | Fremgangsmåder og produkter til produktion af manipulerede mammale cellelinier med forstærkede transgener |
Country Status (5)
| Country | Link |
|---|---|
| US (5) | US20140179005A1 (da) |
| EP (3) | EP3683319A1 (da) |
| DK (2) | DK2714936T3 (da) |
| ES (2) | ES2773891T3 (da) |
| WO (1) | WO2012167192A2 (da) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116622704A (zh) * | 2012-07-25 | 2023-08-22 | 布罗德研究所有限公司 | 可诱导的dna结合蛋白和基因组干扰工具及其应用 |
| AU2015229299A1 (en) | 2014-03-12 | 2016-09-22 | Precision Biosciences, Inc. | Dystrophin gene exon deletion using engineered nucleases |
| SG10201808811QA (en) | 2014-04-10 | 2018-11-29 | Seattle Childrens Hospital Dba Seattle Childrens Res Inst | Method and compositions for cellular immunotherapy |
| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| EP3209785B1 (en) | 2014-10-23 | 2019-07-31 | Regeneron Pharmaceuticals, Inc. | Novel cho integration sites and uses thereof |
| US20180344817A1 (en) | 2015-05-01 | 2018-12-06 | Precision Biosciences, Inc. | Precise deletion of chromosomal sequences in vivo and treatment of nucleotide repeat expansion disorders using engineered nucleases |
| MX2018001568A (es) | 2015-08-07 | 2019-04-25 | Seattle Children´S Hospital Dba Seattle Children´S Res Institute | Celulas t biespecificas de receptor quimerico de antigeno (car) para focalizacion a tumores solidos. |
| CA2997909A1 (en) | 2015-09-08 | 2017-03-16 | Precision Biosciences, Inc. | Treatment of retinitis pigmentosa using engineered meganucleases |
| WO2017051347A2 (en) | 2015-09-23 | 2017-03-30 | Pfizer Inc. | Cells and method of cell culture |
| ES2803728T3 (es) | 2015-10-05 | 2021-01-29 | Prec Biosciences Inc | Células genéticamente modificadas que comprenden un gen de región constante alfa del receptor de linfocitos T humano modificado |
| ES2768984T3 (es) | 2015-10-05 | 2020-06-24 | Prec Biosciences Inc | Meganucleasas diseñadas con secuencias de reconocimiento encontradas en el gen de la región constante alfa del receptor de células T humanas |
| US20190017075A1 (en) | 2015-12-23 | 2019-01-17 | Precision Biosciences, Inc. | Engineered meganucleases with recognition sequences found in the human beta-2 microglobulin gene |
| WO2017173043A1 (en) * | 2016-03-30 | 2017-10-05 | Spark Therapeutics, Inc. | Cell line for recombinant protein and/or viral vector production |
| EP3448380B1 (en) | 2016-04-27 | 2021-12-01 | University Of Puerto Rico | 1,5-disubstituted 1,2,3-triazoles are inhibitors of rac/cdc42 gtpases |
| EP3452583B1 (en) | 2016-05-03 | 2021-10-27 | Precision Biosciences, Inc. | Engineered nucleases useful for treatment of hemophilia a |
| KR20230010826A (ko) | 2016-10-14 | 2023-01-19 | 프리시젼 바이오사이언시스 인코포레이티드 | B형 간염 바이러스 게놈 내의 인식 서열에 대해 특이적인 조작된 메가뉴클레아제 |
| WO2018167621A1 (en) * | 2017-03-16 | 2018-09-20 | Pfizer Inc. | Tyrosine prototrophy |
| EP4269596A3 (en) | 2017-04-21 | 2024-02-14 | Precision Biosciences, Inc. | Engineered meganucleases specific for recognition sequences in the pcsk9 gene |
| EP4775669A2 (en) | 2017-06-30 | 2026-07-15 | Precision Biosciences, Inc. | Genetically-modified t cells comprising a modified intron in the t cell receptor alpha gene |
| EP3704238B1 (en) | 2017-11-01 | 2024-01-03 | Precision Biosciences, Inc. | Engineered nucleases that target human and canine factor viii genes as a treatment for hemophilia a |
| KR102617818B1 (ko) | 2018-04-12 | 2023-12-27 | 프리시젼 바이오사이언시스 인코포레이티드 | 인간 t 세포 수용체 알파 불변 영역 유전자에 대한 특이성을 갖는 최적화된 조작된 뉴클레아제 |
| US11142750B2 (en) | 2018-04-12 | 2021-10-12 | Precision Biosciences, Inc. | Optimized engineered meganucleases having specificity for a recognition sequence in the Hepatitis B virus genome |
| EP3898661A1 (en) | 2018-12-21 | 2021-10-27 | Precision BioSciences, Inc. | Genetic modification of the hydroxyacid oxidase 1 gene for treatment of primary hyperoxaluria |
| CN114026228A (zh) | 2019-05-07 | 2022-02-08 | 精密生物科学公司 | 用于识别序列的工程化大范围核酸酶的优化 |
| US20220273715A1 (en) * | 2019-07-25 | 2022-09-01 | Precision Biosciences, Inc. | Compositions and methods for sequential stacking of nucleic acid sequences into a genomic locus |
| EP4007814A1 (en) * | 2019-07-26 | 2022-06-08 | Akouos, Inc. | Methods of treating hearing loss using a secreted target protein |
| EP4065709A4 (en) * | 2019-11-25 | 2023-11-29 | Baylor College of Medicine | SELECTIVE EXPANSION OF GENE-TARGETED CELLS |
| US12410418B2 (en) | 2019-12-06 | 2025-09-09 | Precision Biosciences, Inc. | Optimized engineered meganucleases having specificity for a recognition sequence in the Hepatitis B virus genome |
| US20230175014A1 (en) * | 2020-04-27 | 2023-06-08 | The Trustees Of The University Of Pennsylvania | Compositions and methods for reducing nuclease expression and off-target activity using a promoter with low transcriptional activity |
| WO2021231259A1 (en) | 2020-05-11 | 2021-11-18 | Precision Biosciences, Inc. | Self-limiting viral vectors encoding nucleases |
| EP4150068A1 (en) | 2020-05-12 | 2023-03-22 | Precision BioSciences, Inc. | Treatment of retinitis pigmentosa using improved engineered meganucleases |
| US20240011003A1 (en) | 2020-08-21 | 2024-01-11 | Precision Biosciences, Inc. | Engineered meganucleases having specificity for a recognition sequence in the transthyretin gene |
| KR20250099412A (ko) | 2020-11-12 | 2025-07-01 | 프리시젼 바이오사이언시스 인코포레이티드 | 디스트로핀 유전자 내의 인식 서열에 대해 특이성을 갖는 조작된 메가뉴클레아제 |
| CA3172161A1 (en) | 2021-01-08 | 2022-07-14 | James Jefferson Smith | Engineered meganucleases having specificity for a recognition sequence in the hydroxyacid oxidase 1 gene |
| WO2022226316A1 (en) | 2021-04-22 | 2022-10-27 | Precision Biosciences, Inc. | Compositions and methods for generating male sterile plants |
| IL307834A (en) | 2021-04-22 | 2023-12-01 | Prec Biosciences Inc | Transgenic meganonucleases targeting human mitochondrial genomes |
| US12134776B2 (en) * | 2021-10-06 | 2024-11-05 | G&P Bioscience Co., Ltd. | Development of optimized recombinant expression construct |
| AU2022368911A1 (en) | 2021-10-19 | 2024-05-30 | Precision Biosciences, Inc. | Gene editing methods for treating alpha-1 antitrypsin (aat) deficiency |
| MX2024004812A (es) | 2021-10-19 | 2024-07-15 | Prec Biosciences Inc | Métodos de edición génica para el tratamiento de la deficiencia de alfa-1 antitripsina (aat). |
| WO2025101946A1 (en) | 2023-11-08 | 2025-05-15 | Precision Biosciences, Inc. | Polypeptide linkers for use in engineered meganucleases |
| WO2025233894A1 (en) | 2024-05-09 | 2025-11-13 | University Of Miami | Engineered meganucleases that target human mitochondrial genomes |
| WO2026028165A1 (en) | 2024-08-01 | 2026-02-05 | Precision Biosciences, Inc. | Engineered meganucleases having specificity for recognition sequences in the c9orf72 gene |
| CN119753013A (zh) * | 2024-11-25 | 2025-04-04 | 杭州宏望医学检验实验室有限公司 | 一种适用于自身抗体检测的cho细胞株的构建及其应用 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6156304A (en) | 1990-12-20 | 2000-12-05 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Gene transfer for studying and treating a connective tissue of a mammalian host |
| US5384253A (en) | 1990-12-28 | 1995-01-24 | Dekalb Genetics Corporation | Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes |
| US7338800B2 (en) * | 2002-01-16 | 2008-03-04 | Baylor College Of Medicine | In vivo gene transfer |
| WO2009095742A1 (en) | 2008-01-31 | 2009-08-06 | Cellectis | New i-crei derived single-chain meganuclease and uses thereof |
| US20060206949A1 (en) * | 2003-01-28 | 2006-09-14 | Sylvain Arnould | Custom-made meganuclease and use thereof |
| DK3246403T3 (da) | 2005-10-18 | 2020-10-26 | Prec Biosciences | Rationelt designede meganucleaser med ændret sekvens-specificitet og dna-bindende affinitet |
| WO2007060495A1 (en) | 2005-10-25 | 2007-05-31 | Cellectis | I-crei homing endonuclease variants having novel cleavage specificity and use thereof |
| EP2019860A4 (en) * | 2006-05-22 | 2010-12-15 | Hiprocell Llc | PROTEIN PRODUCTION USING EUKARYOTIC CELL LINES |
| WO2008059317A1 (en) | 2006-11-14 | 2008-05-22 | Cellectis | Meganuclease variants cleaving a dna target sequence from the hprt gene and uses thereof |
| FR2910490B1 (fr) * | 2006-12-20 | 2012-10-26 | Lab Francais Du Fractionnement | Lignee cellulaire a forte activite transcriptionnelle pour la production de proteines, notamment therapeutiques |
| ES2422291T3 (es) | 2007-10-31 | 2013-09-10 | Prec Biosciences Inc | Meganucleasas monocatenarias diseñadas racionalmente con secuencias de reconocimiento no palindrómicas |
| WO2009076292A2 (en) | 2007-12-07 | 2009-06-18 | Precision Biosciences, Inc. | Rationally-designed meganucleases with recognition sequences found in dnase hypersensitive regions of the human genome |
| WO2009114321A2 (en) * | 2008-03-11 | 2009-09-17 | Precision Biosciencs, Inc. | Rationally-designed meganucleases for maize genome engineering |
| US9273296B2 (en) * | 2008-09-08 | 2016-03-01 | Cellectis | Meganuclease variants cleaving a DNA target sequence from a glutamine synthetase gene and uses thereof |
| EP2180058A1 (en) * | 2008-10-23 | 2010-04-28 | Cellectis | Meganuclease recombination system |
-
2012
- 2012-06-01 EP EP19218660.9A patent/EP3683319A1/en not_active Withdrawn
- 2012-06-01 WO PCT/US2012/040599 patent/WO2012167192A2/en not_active Ceased
- 2012-06-01 ES ES18211367T patent/ES2773891T3/es active Active
- 2012-06-01 EP EP18211367.0A patent/EP3489366B1/en active Active
- 2012-06-01 DK DK12792301.9T patent/DK2714936T3/da active
- 2012-06-01 EP EP12792301.9A patent/EP2714936B1/en active Active
- 2012-06-01 DK DK18211367.0T patent/DK3489366T3/da active
- 2012-06-01 ES ES12792301T patent/ES2713529T3/es active Active
-
2013
- 2013-11-27 US US14/091,572 patent/US20140179005A1/en not_active Abandoned
-
2015
- 2015-07-22 US US14/806,175 patent/US9822381B2/en not_active Expired - Fee Related
-
2017
- 2017-10-13 US US15/783,243 patent/US20180163233A1/en not_active Abandoned
-
2019
- 2019-01-25 US US16/258,207 patent/US20190249199A1/en not_active Abandoned
-
2022
- 2022-01-27 US US17/585,694 patent/US20220333137A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ES2713529T3 (es) | 2019-05-22 |
| WO2012167192A3 (en) | 2014-05-08 |
| EP2714936B1 (en) | 2018-12-12 |
| US20140179005A1 (en) | 2014-06-26 |
| EP2714936A2 (en) | 2014-04-09 |
| EP2714936A4 (en) | 2015-07-29 |
| EP3683319A1 (en) | 2020-07-22 |
| ES2773891T3 (es) | 2020-07-15 |
| US20190249199A1 (en) | 2019-08-15 |
| US9822381B2 (en) | 2017-11-21 |
| US20160017372A1 (en) | 2016-01-21 |
| US20180163233A1 (en) | 2018-06-14 |
| WO2012167192A2 (en) | 2012-12-06 |
| EP3489366A1 (en) | 2019-05-29 |
| DK2714936T3 (da) | 2019-03-25 |
| EP3489366B1 (en) | 2019-12-25 |
| US20220333137A1 (en) | 2022-10-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK2872629T3 (da) | Tm-forstærkede blokeringsoligonukleotider og lokkemidler til forbedret target-berigesle og reduceret off-target-udvælgelse | |
| DK3045187T3 (da) | Injektor og fremgangsmåde til samling | |
| DK2756073T3 (da) | Manipuleret væv til in vitro-forskningsanvendelser, anordninger deraf og fremgangsmåder til fremstilling deraf | |
| DK3468197T3 (da) | Fremgangsmåde til interforudsigelse og indretning dertil | |
| DK2950535T3 (da) | Fremgangsmåde og indretning til at afkode intra-forudsigelsestilstand | |
| EP2880054A4 (en) | HLA-G-MODIFIED CELLS AND METHOD | |
| DK3257943T3 (da) | Fremgangsmåder og vektorer til celleimmortalisering | |
| DK3482765T3 (da) | Langtidsvirkende væksthormon og fremgangsmåder til produktion deraf | |
| DK3308641T3 (da) | Immundeficient genmodificeret gnaver og fremgangsmåder til anvendelse deraf | |
| DK2678283T3 (da) | Vanadiumbaserede frittematerialer og fremgangsmåder til deres fremstilling | |
| PL3863288T3 (pl) | Sposoby intra-predykcji i środek nośnikowy | |
| DK2794907T3 (da) | Fremgangsmåder og materialer til vurdering af tab af heterozygositet | |
| DK2758349T3 (da) | Vanadiumbaserede frittematerialer og fremgangsmåder til fremstilling heraf | |
| DK2528180T3 (da) | Metoder og systemer til jævnstrøms-energitransmission | |
| DK2794850T3 (da) | Bioteknologisk produktion af alkoholer og derivater deraf | |
| DK2506416T3 (da) | System og fremgangsmåde til strømkonvertering | |
| DK3489685T3 (da) | Signalforstærkning i sidestrømning og tilsvarende immunoassays | |
| DK3578201T3 (da) | Fremgangsmåder og sammensætninger til naturlige dræberceller | |
| DK2867600T3 (da) | System og fremgangsmåde til carbondioxidopfangning og -deponering | |
| DK2915064T3 (da) | Fremgangsmåde til at indikere tilstedeværelsen af gashydrat og overfladegas i et undervandsmiljø | |
| IL230469A0 (en) | Curable compositions and membranes | |
| DK2521221T4 (da) | Høreanordning og fremgangsmåde | |
| DK2744456T3 (da) | Forbedringer ved eller i forbindelse med proteser og ortoser | |
| DK3415157T3 (da) | N-acyldipeptidderivater og anvendelser deraf | |
| DK2925782T3 (da) | Anti-cd47-antistoffer og fremgangsmåder til anvendelse deraf |