DK3489366T3 - Fremgangsmåder og produkter til produktion af manipulerede mammale cellelinier med forstærkede transgener - Google Patents
Fremgangsmåder og produkter til produktion af manipulerede mammale cellelinier med forstærkede transgener Download PDFInfo
- Publication number
- DK3489366T3 DK3489366T3 DK18211367.0T DK18211367T DK3489366T3 DK 3489366 T3 DK3489366 T3 DK 3489366T3 DK 18211367 T DK18211367 T DK 18211367T DK 3489366 T3 DK3489366 T3 DK 3489366T3
- Authority
- DK
- Denmark
- Prior art keywords
- transgenes
- amplified
- products
- methods
- cell lines
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
- C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
- C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
- C12Y105/01003—Dihydrofolate reductase (1.5.1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
- C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
- C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/04—Phosphoric diester hydrolases (3.1.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161492174P | 2011-06-01 | 2011-06-01 | |
| EP12792301.9A EP2714936B1 (en) | 2011-06-01 | 2012-06-01 | Methods and products for producing engineered mammalian cell lines with amplified transgenes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3489366T3 true DK3489366T3 (da) | 2020-02-24 |
Family
ID=47260412
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12792301.9T DK2714936T3 (da) | 2011-06-01 | 2012-06-01 | Fremgangsmåder og produkter til produktion af manipulerede mammale cellelinier med forstærkede transgener |
| DK18211367.0T DK3489366T3 (da) | 2011-06-01 | 2012-06-01 | Fremgangsmåder og produkter til produktion af manipulerede mammale cellelinier med forstærkede transgener |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12792301.9T DK2714936T3 (da) | 2011-06-01 | 2012-06-01 | Fremgangsmåder og produkter til produktion af manipulerede mammale cellelinier med forstærkede transgener |
Country Status (5)
| Country | Link |
|---|---|
| US (5) | US20140179005A1 (da) |
| EP (3) | EP2714936B1 (da) |
| DK (2) | DK2714936T3 (da) |
| ES (2) | ES2713529T3 (da) |
| WO (1) | WO2012167192A2 (da) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2013293270B2 (en) * | 2012-07-25 | 2018-08-16 | Massachusetts Institute Of Technology | Inducible DNA binding proteins and genome perturbation tools and applications thereof |
| EP4659765A3 (en) | 2014-03-12 | 2026-02-18 | Precision Biosciences, Inc. | Dystrophin gene exon deletion using engineered nucleases |
| US10611837B2 (en) | 2014-04-10 | 2020-04-07 | Seattle Children's Hospital | Transgene genetic tags and methods of use |
| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| BR112017008022A2 (pt) | 2014-10-23 | 2018-02-20 | Regeneron Pharmaceuticals, Inc. | célula, polinucleotídeo, veículo, e, métodos para modificação de um genoma de células cho e para preparação de uma proteína de interesse. |
| ES2905181T3 (es) | 2015-05-01 | 2022-04-07 | Prec Biosciences Inc | Deleción precisa de secuencias cromosómicas in vivo |
| AU2016306209B2 (en) | 2015-08-07 | 2023-07-06 | Seattle Children's Hospital (dba Seattle Children's Research Institute) | Bispecific CAR T-cells for solid tumor targeting |
| US10603363B2 (en) * | 2015-09-08 | 2020-03-31 | Precision Biosciences, Inc. | Treatment of retinitis pigmentosa using engineered meganucleases |
| JP7001585B2 (ja) | 2015-09-23 | 2022-02-04 | ファイザー・インク | 細胞および細胞培養の方法 |
| JP6816133B2 (ja) | 2015-10-05 | 2021-01-20 | プレシジョン バイオサイエンシズ，インク． | 改変ヒトｔ細胞受容体アルファ定常領域遺伝子を含む遺伝子改変細胞 |
| EP3359660B1 (en) | 2015-10-05 | 2019-12-04 | Precision Biosciences, Inc. | Engineered meganucleases with recognition sequences found in the human t cell receptor alpha constant region gene |
| CA3009637A1 (en) | 2015-12-23 | 2017-06-29 | Precision Biosciences, Inc. | Engineered meganucleases with recognition sequences found in the human beta-2 microglobulin gene |
| WO2017173043A1 (en) * | 2016-03-30 | 2017-10-05 | Spark Therapeutics, Inc. | Cell line for recombinant protein and/or viral vector production |
| ES2907837T3 (es) | 2016-04-27 | 2022-04-26 | Univ Puerto Rico | 1,2,3-triazoles 1,5-disustituidos son inhibidores de las GTPasas Rac/Cdc42 |
| DK3452583T3 (da) | 2016-05-03 | 2022-01-10 | Prec Biosciences Inc | Konstruerede nukleaser, der er nyttige til behandling af hæmofili a |
| KR20230010826A (ko) | 2016-10-14 | 2023-01-19 | 프리시젼 바이오사이언시스 인코포레이티드 | B형 간염 바이러스 게놈 내의 인식 서열에 대해 특이적인 조작된 메가뉴클레아제 |
| WO2018167621A1 (en) * | 2017-03-16 | 2018-09-20 | Pfizer Inc. | Tyrosine prototrophy |
| IL301059B2 (en) | 2017-04-21 | 2026-04-01 | Prec Biosciences Inc | Engineered nucleosides specific for recognition sequences in the PCSK9 gene |
| EP3645038B1 (en) | 2017-06-30 | 2026-02-18 | Precision Biosciences, Inc. | Genetically-modified t cells comprising a modified intron in the t cell receptor alpha gene |
| WO2019089913A1 (en) | 2017-11-01 | 2019-05-09 | Precision Biosciences, Inc. | Engineered nucleases that target human and canine factor viii genes as a treatment for hemophilia a |
| US11786554B2 (en) | 2018-04-12 | 2023-10-17 | Precision Biosciences, Inc. | Optimized engineered nucleases having specificity for the human T cell receptor alpha constant region gene |
| US11142750B2 (en) | 2018-04-12 | 2021-10-12 | Precision Biosciences, Inc. | Optimized engineered meganucleases having specificity for a recognition sequence in the Hepatitis B virus genome |
| WO2020132659A1 (en) | 2018-12-21 | 2020-06-25 | Precision Biosciences, Inc. | Genetic modification of the hydroxyacid oxidase 1 gene for treatment of primary hyperoxaluria |
| CN114026228A (zh) | 2019-05-07 | 2022-02-08 | 精密生物科学公司 | 用于识别序列的工程化大范围核酸酶的优化 |
| US20220273715A1 (en) * | 2019-07-25 | 2022-09-01 | Precision Biosciences, Inc. | Compositions and methods for sequential stacking of nucleic acid sequences into a genomic locus |
| US20230001020A1 (en) * | 2019-11-25 | 2023-01-05 | Baylor College Of Medicine | Selective expansion of gene-targeted cells |
| DK4069729T3 (da) | 2019-12-06 | 2025-04-07 | Prec Biosciences Inc | Optimerede, modificerede meganukleaser med specificitet for en genkendelsessekvens i hepatitis b-virusgenomet |
| US20230175014A1 (en) * | 2020-04-27 | 2023-06-08 | The Trustees Of The University Of Pennsylvania | Compositions and methods for reducing nuclease expression and off-target activity using a promoter with low transcriptional activity |
| US20230183664A1 (en) | 2020-05-11 | 2023-06-15 | Precision Biosciences, Inc. | Self-limiting viral vectors encoding nucleases |
| CA3172171A1 (en) | 2020-05-12 | 2021-11-18 | Victor Bartsevich | Treatment of retinitis pigmentosa using improved engineered meganucleases |
| US20240011003A1 (en) | 2020-08-21 | 2024-01-11 | Precision Biosciences, Inc. | Engineered meganucleases having specificity for a recognition sequence in the transthyretin gene |
| JP7642809B2 (ja) | 2020-11-12 | 2025-03-10 | プレシジョン バイオサイエンシズ，インク． | ジストロフィン遺伝子内の認識配列に特異性を有する遺伝子操作されたメガヌクレアーゼ |
| WO2022150616A1 (en) | 2021-01-08 | 2022-07-14 | Precision Biosciences, Inc. | Engineered meganucleases having specificity for a recognition sequence in the hydroxyacid oxidase 1 gene |
| US20240141311A1 (en) | 2021-04-22 | 2024-05-02 | North Carolina State University | Compositions and methods for generating male sterile plants |
| WO2022226305A1 (en) | 2021-04-22 | 2022-10-27 | Precision Biosciences, Inc. | Engineered meganucleases that target human mitochondrial genomes |
| US12134776B2 (en) * | 2021-10-06 | 2024-11-05 | G&P Bioscience Co., Ltd. | Development of optimized recombinant expression construct |
| IL312244A (en) | 2021-10-19 | 2024-06-01 | Prec Biosciences Inc | Gene editing methods for treating alpha-1 antitrypsin (aat) deficiency |
| WO2023070002A2 (en) | 2021-10-19 | 2023-04-27 | Precision Biosciences, Inc. | Gene editing methods for treating alpha-1 antitrypsin (aat) deficiency |
| WO2025101946A1 (en) | 2023-11-08 | 2025-05-15 | Precision Biosciences, Inc. | Polypeptide linkers for use in engineered meganucleases |
| WO2025233894A1 (en) | 2024-05-09 | 2025-11-13 | University Of Miami | Engineered meganucleases that target human mitochondrial genomes |
| WO2026028165A1 (en) | 2024-08-01 | 2026-02-05 | Precision Biosciences, Inc. | Engineered meganucleases having specificity for recognition sequences in the c9orf72 gene |
| CN119753013A (zh) * | 2024-11-25 | 2025-04-04 | 杭州宏望医学检验实验室有限公司 | 一种适用于自身抗体检测的cho细胞株的构建及其应用 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6156304A (en) | 1990-12-20 | 2000-12-05 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Gene transfer for studying and treating a connective tissue of a mammalian host |
| US5384253A (en) | 1990-12-28 | 1995-01-24 | Dekalb Genetics Corporation | Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes |
| US7338800B2 (en) * | 2002-01-16 | 2008-03-04 | Baylor College Of Medicine | In vivo gene transfer |
| WO2009095742A1 (en) | 2008-01-31 | 2009-08-06 | Cellectis | New i-crei derived single-chain meganuclease and uses thereof |
| EP3202899B1 (en) * | 2003-01-28 | 2020-10-21 | Cellectis | Custom-made meganuclease and use thereof |
| ES2582091T3 (es) | 2005-10-18 | 2016-09-09 | Precision Biosciences | Meganucleasas diseñadas racionalmente con especificidad de secuencia y afinidad de unión a ADN alteradas |
| WO2007060495A1 (en) | 2005-10-25 | 2007-05-31 | Cellectis | I-crei homing endonuclease variants having novel cleavage specificity and use thereof |
| NZ572884A (en) * | 2006-05-22 | 2011-12-22 | Hiprocell Llc | Transformation of eukaryotic cells using two different recombination sites recognised by two different unidirectional site-specific recombinases |
| WO2008059317A1 (en) | 2006-11-14 | 2008-05-22 | Cellectis | Meganuclease variants cleaving a dna target sequence from the hprt gene and uses thereof |
| FR2910490B1 (fr) * | 2006-12-20 | 2012-10-26 | Lab Francais Du Fractionnement | Lignee cellulaire a forte activite transcriptionnelle pour la production de proteines, notamment therapeutiques |
| EP2215223B1 (en) | 2007-10-31 | 2013-05-01 | Precision Biosciences, Inc. | Rationally-designed single-chain meganucleases with non-palindromic recognition sequences |
| JP2011505809A (ja) | 2007-12-07 | 2011-03-03 | プレシジョン バイオサイエンシズ，インク． | ヒトゲノムのＤＮａｓｅ高感受性領域に見出される認識配列を有する合理的に設計されたメガヌクレアーゼ |
| WO2009114321A2 (en) * | 2008-03-11 | 2009-09-17 | Precision Biosciencs, Inc. | Rationally-designed meganucleases for maize genome engineering |
| CN102177235A (zh) * | 2008-09-08 | 2011-09-07 | 赛莱克蒂斯公司 | 切割来自谷氨酰胺合成酶基因的dna靶序列的大范围核酸酶变体及其用途 |
| EP2180058A1 (en) * | 2008-10-23 | 2010-04-28 | Cellectis | Meganuclease recombination system |
-
2012
- 2012-06-01 EP EP12792301.9A patent/EP2714936B1/en active Active
- 2012-06-01 DK DK12792301.9T patent/DK2714936T3/da active
- 2012-06-01 WO PCT/US2012/040599 patent/WO2012167192A2/en not_active Ceased
- 2012-06-01 ES ES12792301T patent/ES2713529T3/es active Active
- 2012-06-01 ES ES18211367T patent/ES2773891T3/es active Active
- 2012-06-01 EP EP19218660.9A patent/EP3683319A1/en not_active Withdrawn
- 2012-06-01 EP EP18211367.0A patent/EP3489366B1/en active Active
- 2012-06-01 DK DK18211367.0T patent/DK3489366T3/da active
-
2013
- 2013-11-27 US US14/091,572 patent/US20140179005A1/en not_active Abandoned
-
2015
- 2015-07-22 US US14/806,175 patent/US9822381B2/en not_active Expired - Fee Related
-
2017
- 2017-10-13 US US15/783,243 patent/US20180163233A1/en not_active Abandoned
-
2019
- 2019-01-25 US US16/258,207 patent/US20190249199A1/en not_active Abandoned
-
2022
- 2022-01-27 US US17/585,694 patent/US20220333137A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012167192A2 (en) | 2012-12-06 |
| US20160017372A1 (en) | 2016-01-21 |
| US9822381B2 (en) | 2017-11-21 |
| WO2012167192A3 (en) | 2014-05-08 |
| EP2714936B1 (en) | 2018-12-12 |
| EP2714936A4 (en) | 2015-07-29 |
| DK2714936T3 (da) | 2019-03-25 |
| EP3489366A1 (en) | 2019-05-29 |
| EP3489366B1 (en) | 2019-12-25 |
| US20220333137A1 (en) | 2022-10-20 |
| US20140179005A1 (en) | 2014-06-26 |
| US20190249199A1 (en) | 2019-08-15 |
| EP3683319A1 (en) | 2020-07-22 |
| US20180163233A1 (en) | 2018-06-14 |
| ES2773891T3 (es) | 2020-07-15 |
| EP2714936A2 (en) | 2014-04-09 |
| ES2713529T3 (es) | 2019-05-22 |
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