DK3633025T3 - Dyrkningsmedier, cellekulturer og metoder til dyrkning af pluripotente stamceller i en udifferentieret tilstand - Google Patents

Dyrkningsmedier, cellekulturer og metoder til dyrkning af pluripotente stamceller i en udifferentieret tilstand Download PDF

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DK3633025T3
DK3633025T3 DK19210132.7T DK19210132T DK3633025T3 DK 3633025 T3 DK3633025 T3 DK 3633025T3 DK 19210132 T DK19210132 T DK 19210132T DK 3633025 T3 DK3633025 T3 DK 3633025T3
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methods
stem cells
culture media
pluripotent stem
cell cultures
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DK19210132.7T
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Michal Amit
Joseph Itskovitz-Eldor
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Technion Res & Dev Foundation
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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DK19210132.7T 2009-11-12 2010-11-11 Dyrkningsmedier, cellekulturer og metoder til dyrkning af pluripotente stamceller i en udifferentieret tilstand DK3633025T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27286009P 2009-11-12 2009-11-12
EP10829622.9A EP2499236B1 (en) 2009-11-12 2010-11-11 Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state

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DK3633025T3 true DK3633025T3 (da) 2022-12-12

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DK19210132.7T DK3633025T3 (da) 2009-11-12 2010-11-11 Dyrkningsmedier, cellekulturer og metoder til dyrkning af pluripotente stamceller i en udifferentieret tilstand
DK10829622.9T DK2499236T3 (da) 2009-11-12 2010-11-11 Dyrkningsmedier, cellekulturer og metoder til dyrkning af pluripotente stamceller i en udifferentieret tilstand

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US (4) US9018010B2 (da)
EP (3) EP4166652A1 (da)
JP (5) JP6276918B2 (da)
CA (3) CA3080762C (da)
DK (2) DK3633025T3 (da)
ES (2) ES2932664T3 (da)
IL (1) IL252607B (da)
WO (1) WO2011058558A2 (da)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011058558A2 (en) * 2009-11-12 2011-05-19 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
AU2011223900A1 (en) 2010-03-01 2012-09-13 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
CN108531444A (zh) 2010-08-05 2018-09-14 威斯康星校友研究基金会 用于人多能细胞培养的简化基础培养基
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
CA3150342A1 (en) 2010-09-07 2012-03-15 Technion Research & Development Foundation Limited Novel methods and culture media for culturing pluripotent stem cells
US9732319B2 (en) 2010-12-22 2017-08-15 Fate Therapeutics, Inc. Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs
EP4086338A1 (en) 2011-03-17 2022-11-09 Minerva Biotechnologies Corporation Method for making pluripotent stem cells
GB201117469D0 (en) * 2011-10-10 2011-11-23 Cell Guidance Systems Ltd Culture media for pluripotent stem cells
EP2768945B1 (en) * 2011-10-17 2022-01-05 Minerva Biotechnologies Corporation Media for stem cell proliferation and induction
WO2013077423A1 (ja) 2011-11-25 2013-05-30 国立大学法人京都大学 多能性幹細胞の培養方法
EP3260140B1 (en) 2011-12-05 2021-02-03 Factor Bioscience Inc. Methods and products for transfecting cells
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
RU2705001C2 (ru) 2011-12-22 2019-11-01 Янссен Байотек, Инк. Дифференцировка эмбриональных стволовых клеток человека в одногормональные инсулинположительные клетки
US20140162359A1 (en) * 2012-05-07 2014-06-12 Janssen Biotech, Inc. Differentiation of Human Embryonic Stem Cells into Pancreatic Endoderm
DK2938722T3 (da) 2012-12-31 2022-02-14 Janssen Biotech Inc Suspension og klyngedannelse af humane pluripotente celler til differentiering til endokrine pankreasceller
KR102084561B1 (ko) 2012-12-31 2020-03-04 얀센 바이오테크 인코포레이티드 췌장 내분비 세포로의 분화를 위한 공기-액체 계면에서의 인간 배아 줄기세포의 배양
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
US9738861B2 (en) 2013-03-06 2017-08-22 Kyoto University Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells
PL3008172T3 (pl) 2013-06-11 2020-09-21 Ncardia B.V. Kompozycje pożywki hodowlanej do dojrzewania kardiomiocytów pochodzących z pluripotencjalnych ssaczych komórek macierzystych
WO2014208295A1 (ja) * 2013-06-28 2014-12-31 株式会社カネカ 多能性幹細胞の増殖促進因子のスクリーニング法
WO2015037622A1 (ja) * 2013-09-10 2015-03-19 富士フイルム株式会社 多能性幹細胞の培養方法、培養用キット及び多能性幹細胞培養用培地
JP6174155B2 (ja) * 2013-09-10 2017-08-02 富士フイルム株式会社 多能性幹細胞の培養方法ならびにこれに用いる多能性幹細胞の培養用キットおよび培地
WO2015098111A1 (ja) * 2013-12-26 2015-07-02 国立大学法人広島大学 ｉＰＳ細胞の樹立方法および幹細胞の長期維持方法
JP6516280B2 (ja) * 2013-12-26 2019-05-22 国立大学法人広島大学 ｉＰＳ細胞の樹立方法および幹細胞の長期維持方法
CN120536341A (zh) 2014-03-04 2025-08-26 菲特治疗公司 改良的重编程方法和细胞培养平台
CA2943035A1 (en) * 2014-05-06 2015-11-12 F. Hoffmann-La Roche Ag Method for differentiation of pluripotent stem cells into cardiomyocytes
US20150335010A1 (en) 2014-05-21 2015-11-26 Bourbon Corporation Maintenance medium for primate pluripotent stem cells
CN104388382B (zh) * 2014-11-21 2017-05-10 吉林大学 利用搅拌式细胞培养转瓶机快速制备饲养层细胞的方法
US9944894B2 (en) 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
CA2996582A1 (en) * 2015-08-31 2017-03-09 I Peace, Inc. Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells
JP6884354B2 (ja) * 2015-09-08 2021-06-09 大日本住友製薬株式会社 網膜組織の製造方法
CN117737124A (zh) 2015-10-16 2024-03-22 菲特治疗公司 用于诱导和维护基态多能性的平台
EP3487987A4 (en) 2016-07-19 2020-02-19 Accellta Ltd. CULTURAL MEDIA FOR THE CULTURE OF PLURIPOTENTAL STEM CELLS IN A SUSPENSION
WO2018029656A2 (en) * 2016-08-12 2018-02-15 The University Of Chicago Methods for making and using therapeutic exosomes
JP6647545B2 (ja) * 2017-05-02 2020-02-14 剛士 田邊 医薬品組成物及び化粧品組成物
CN111886334A (zh) * 2018-03-20 2020-11-03 富士胶片株式会社 三维培养多能干细胞的方法
CN115103903A (zh) * 2019-09-19 2022-09-23 西北大学 用于人诱导型多能干细胞的具成本效益的培养基和操作方案
CA3160433A1 (en) * 2019-12-09 2021-06-17 Daewoong Pharmaceutical Co., Ltd. Method for preparation of mesenchymal stem cell from human pluripotent stem cell and mesenchymal stem cells prepared thereby
CA3167723A1 (en) * 2020-01-14 2021-07-22 Ajinomoto Co., Inc. Cell culture medium composition
CN111484970B (zh) * 2020-04-30 2022-09-16 广州再生医学与健康广东省实验室 一种低蛋白含量的无血清无饲养层胚胎与多能干细胞培养基
JP2024520792A (ja) * 2021-06-09 2024-05-24 アクセルタ リミテッド 哺乳類家畜多能性幹細胞の懸濁培養のための無血清培地
US11735303B2 (en) 2021-06-22 2023-08-22 David Haase Apparatus and method for determining a composition of a replacement therapy treatment
CN113403262A (zh) * 2021-06-23 2021-09-17 昆明理工大学 一种对食蟹猴干细胞的无饲养层培养方法
WO2023053220A1 (ja) * 2021-09-28 2023-04-06 公益財団法人京都大学ｉＰＳ細胞研究財団 多能性幹細胞の製造方法
AU2023319978A1 (en) * 2022-08-02 2025-01-30 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Bovine trophoblast stem cells and uses thereof
USD1069165S1 (en) 2022-11-02 2025-04-01 Abs Global, Inc. Dish holder
WO2024218715A1 (en) 2023-04-19 2024-10-24 Btl Healthcare Technologies A.S. Culture media based on protein hydrolysate and a process for preparing thereof
US12668774B2 (en) 2023-10-12 2026-06-30 Btl Healthcare Technologies A.S. Product, system and method of cell cultivation

Family Cites Families (192)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
IL68218A (en) 1983-03-23 1985-12-31 Univ Ramot Compositions for cartilage repair comprising embryonal chondrocytes
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5489304A (en) 1994-04-19 1996-02-06 Brigham & Women's Hospital Method of skin regeneration using a collagen-glycosaminoglycan matrix and cultured epithelial autograft
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US7410773B2 (en) 1995-02-02 2008-08-12 Ghazi Jaswinder Dhoot Method of preparing an undifferentiated cell
AU7243100A (en) 1995-02-02 2001-05-03 Tristem Trading (Cyprus) Limited A method of preparing an undifferentiated cell
JPH08308561A (ja) * 1995-05-16 1996-11-26 Sumitomo Electric Ind Ltd 動物細胞培養用無血清培地
US5811297A (en) 1996-03-07 1998-09-22 Amba Biosciences, Llc Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages
JP4307552B2 (ja) 1996-03-15 2009-08-05 ミューニン コーポレイション 細胞外マトリックスシグナリング分子
US6878544B2 (en) 1996-04-19 2005-04-12 Neurotech Sa Retinal cell lines with extended life-span and their applications
JP2001508302A (ja) 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド 胚性幹細胞血清置換
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
US6479285B1 (en) 1997-07-02 2002-11-12 Board Of Regents, The University Of Texas System p53 as a regulator of cell differentiation
IL122818A0 (en) 1997-07-10 1998-08-16 Yeda Res & Dev Chimeric interleukin-6 soluble receptor/ligand protein analogs thereof and uses thereof
WO1999020740A2 (en) 1997-10-23 1999-04-29 Geron Corporation Methods and materials for the growth of primate-derived primordial stem cells
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US7413904B2 (en) 1998-10-23 2008-08-19 Geron Corporation Human embryonic stem cells having genetic modifications
IL142914A0 (en) * 1998-11-09 2002-04-21 Consorzio Per La Gestione Del Serum free medium for chondrocyte-like cells
EP1129176A4 (en) 1998-11-09 2002-10-30 Es Cell Int Pte Ltd EMBRYONIC STEM CELLS
US20030129745A1 (en) 1999-10-28 2003-07-10 Robl James M. Gynogenetic or androgenetic production of pluripotent cells and cell lines, and use thereof to produce differentiated cells and tissues
US7455983B2 (en) 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
JP2001229797A (ja) 2000-02-15 2001-08-24 Yazaki Corp ガス式電源遮断装置
WO2001068815A1 (en) 2000-03-14 2001-09-20 Es Cell International Pte Ltd Embryonic stem cells and neural progenitor cells derived therefrom
AU2001244354A1 (en) 2000-03-30 2001-10-15 Oxford Biomedica (Uk) Limited Retinoic acid receptor beta-2, its antagonists, and gene therapy vectors for thetreatment of neurological disorders
US7473555B2 (en) 2000-04-27 2009-01-06 Geron Corporation Protocols for making hepatocytes from embryonic stem cells
NL1017973C2 (nl) 2000-05-10 2002-11-08 Tristem Trading Cyprus Ltd Inrichting.
EP1287116A2 (en) 2000-05-17 2003-03-05 Geron Corporation Neural progenitor cell populations
US7250294B2 (en) 2000-05-17 2007-07-31 Geron Corporation Screening small molecule drugs using neural cells differentiated from human embryonic stem cells
US20020090722A1 (en) 2000-06-15 2002-07-11 Tanja Dominko Pluripotent mammalian cells
GB0025088D0 (en) 2000-10-13 2000-11-29 Univ Edinburgh Stem cells
EP1379624A2 (en) 2000-11-27 2004-01-14 Yissum Research Development Company Of The Hebrew University Of Jerusalem Transfection of human embryonic stem cells
US6576464B2 (en) 2000-11-27 2003-06-10 Geron Corporation Methods for providing differentiated stem cells
KR20030088022A (ko) 2000-11-30 2003-11-15 스템론 인크. 단리된 동종접합 간세포, 그로부터 유래된 분화 세포 및이들을 제조 및 사용하기 위한 물질 및 방법
WO2002092756A2 (en) 2001-05-15 2002-11-21 Rappaport Family Institute For Research In The Medical Sciences Insulin producing cells derived from human embryonic stem cells
IL159578A0 (en) 2001-07-06 2004-06-01 Geron Corp Messenchymal cells and osteoblasts from human embryonic stem cell
EP1412479A4 (en) 2001-07-12 2004-07-28 Geron Corp CELLS WITH CARDIOMYOCYTE GENERATION PRODUCED FROM HUMAN PLURIPOTENT STEM CELLS
CA2452256A1 (en) 2001-07-24 2003-02-06 Es Cell International Pte Ltd Methods of inducing differentiation of stem cells
US7432104B2 (en) 2001-08-06 2008-10-07 Bresgen Inc. Methods for the culture of human embryonic stem cells on human feeder cells
US20030211603A1 (en) 2001-08-14 2003-11-13 Earp David J. Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells
US20030044976A1 (en) 2001-08-27 2003-03-06 Advanced Cell Technology De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
AU2002340897A1 (en) 2001-09-12 2003-03-24 Develogen Aktiengesellschaft Fur Entwicklungsbiologische Forschung A method for isolating, culturing and differentiating intestinal stem cells for therapeutic use
US20050095708A1 (en) 2001-11-09 2005-05-05 Pera Martin F. Characterization and isolation of subsets of human embryonic stem cells (HES) and cells associated or derived therefrom
GB2399823B (en) 2001-12-07 2006-02-15 Geron Corp Islet cells from primate pluripotent stem cells
CN1630716A (zh) 2001-12-07 2005-06-22 杰龙公司 人胚胎干细胞衍生的造血细胞
JP2005511083A (ja) 2001-12-07 2005-04-28 ジェロン コーポレイション ヒト胚幹細胞由来の軟骨細胞前駆細胞
ATE501167T1 (de) 2001-12-21 2011-03-15 Thrombogenics Nv Zusammensetzungen für die in vitro herleitung und kultur embryonaler stammzellinien mit keimbahnübertragungsfähigkeit und für die kultur adulter stammzellen
CN1671835A (zh) 2001-12-28 2005-09-21 塞拉提斯股份公司 一种建立多能人胚泡衍生的干细胞的方法
AU2009213101B2 (en) 2002-06-05 2012-03-08 Es Cell International Pte Ltd Generation of neural stem cells from undifferentiated human embryonic stem cells
WO2003104423A2 (en) 2002-06-11 2003-12-18 Roy Ogle Meningeal-derived stem cells
US7662625B2 (en) 2002-07-02 2010-02-16 Cancer Research Technology Limited Methods for detecting the differentiation status of cells using 5T4 antigen expression
US20050101014A1 (en) 2002-07-11 2005-05-12 Keirstead Hans S. Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US7285415B2 (en) 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US7267981B2 (en) 2002-10-07 2007-09-11 Technion Research & Development Foundation Ltd. Human foreskin fibroblasts for culturing ES cells
US20060252150A1 (en) 2002-11-08 2006-11-09 Linzhao Cheng Human embryonic stem cell cultures, and compositions and methods for growing same
US20040110286A1 (en) 2002-12-06 2004-06-10 The John P. Robarts Research Institute Method for making hematopoietic cells
PT1572984E (pt) 2002-12-16 2016-06-03 Technion Res & Dev Foundation Sistema de cultura para células estaminais embrionárias humanas sem células alimentadoras e sem xeno
WO2004078944A2 (en) 2003-03-05 2004-09-16 Maine Medical Center Research Institute Thrombin, soluble jaggedi, and trap and growth of stem cells
US7153650B2 (en) 2003-03-13 2006-12-26 Geron Corporation Marker system for preparing and characterizing high-quality human embryonic stem cells
US20030224411A1 (en) 2003-03-13 2003-12-04 Stanton Lawrence W. Genes that are up- or down-regulated during differentiation of human embryonic stem cells
US20060134636A1 (en) 2003-03-13 2006-06-22 Stanton Lawrence W Standardization of growth conditions for human embryonic stem cells intended for use in regenerative medicine
CA2520722A1 (en) 2003-03-28 2004-10-07 Sunnybrook And Women's College Health Sciences Centre Canine embryonic stem cells
EP1625213A2 (en) 2003-05-08 2006-02-15 Cellartis AB A method for efficient transfer of human blastocyst-derived stem cells (hbs cells) from a feeder-supported to a feeder-free culture system
US20050118713A1 (en) 2003-05-12 2005-06-02 Nikolai Strelchenko Morula derived embryonic stem cells
US20040229350A1 (en) 2003-05-12 2004-11-18 Nikolai Strelchenko Morula derived embryonic stem cells
US7569385B2 (en) 2003-08-14 2009-08-04 The Regents Of The University Of California Multipotent amniotic fetal stem cells
EP1694825A4 (en) 2003-12-01 2008-06-18 Technion Res & Dev Foundation METHODS OF GENERATING STEM CELLS AND EMBRYONIC BODIES HAVING MUTATIONS THAT CAUSE DISEASES AND METHODS OF USE FOR STUDYING GENETIC DISORDERS
TWI280280B (en) 2003-12-09 2007-05-01 Ind Tech Res Inst Culture system and method for expansion and undifferentiated growth of human embryonic stem cells
US20050233446A1 (en) * 2003-12-31 2005-10-20 Parsons Xuejun H Defined media for stem cell culture
WO2005080551A2 (en) 2004-02-12 2005-09-01 University Of Newcastle Upon Tyne Stem cells
US20060216821A1 (en) 2004-02-26 2006-09-28 Reliance Life Sciences Pvt. Ltd. Pluripotent embryonic-like stem cells derived from corneal limbus, methods of isolation and uses thereof
US20090155218A1 (en) 2004-03-10 2009-06-18 Alberto Hayek Compositions and methods for growth of embryonic stem cells
GB2427873B (en) 2004-03-19 2008-09-10 Geron Corp Method for making high purity cardiomyocyte preparations suitable for regenerative medicine
IL178006A (en) 2004-03-19 2011-08-31 Geron Corp Method for making high purity cardiomyocyte preparations suitable for regenerative medicine
SG150567A1 (en) 2004-03-23 2009-03-30 Akaike Toshihiro Pluripotent stem cell growing method
US20050214938A1 (en) 2004-03-26 2005-09-29 Gold Joseph D Cardiac bodies: clusters of spontaneously contracting cells for regenerating cardiac function
US7452718B2 (en) 2004-03-26 2008-11-18 Geron Corporation Direct differentiation method for making cardiomyocytes from human embryonic stem cells
US20050227352A1 (en) 2004-04-09 2005-10-13 Stowers Institute For Medical Research Use of BMP (BAM) signaling to control stem cell fate determination
JP5128946B2 (ja) 2004-05-21 2013-01-23 ウィセル リサーチ インスティテュート インコーポレイテッド 胚性幹細胞のフィーダー非依存性長期培養
WO2005113747A2 (de) 2004-05-21 2005-12-01 Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V. Multizelluläre gewebe- und organkultursysteme
US20080038820A1 (en) 2004-06-22 2008-02-14 Rudy-Reil Diane E Induction of pluripotent stem cells into mesodermal lineages
US20090136559A1 (en) 2004-07-09 2009-05-28 Athanasiou Kyriacos A Chondrocyte Differentiation from Human Embryonic Stem Cells and Their Use in Tissue Engineering
AU2005271723B2 (en) 2004-07-13 2010-12-16 Asterias Biotherapeutics, Inc. Medium for growing human embryonic stem cells
CA2504451A1 (en) 2004-08-10 2006-02-10 Geron Corporation Dendritic cell vaccines for treating cancer made from embryonic stem cells
WO2006017855A2 (en) 2004-08-12 2006-02-16 Board Of Regents Of The University Of Texas System Methods and compositions for regulation of stem cell survival, proliferation, and differentiation by protein ubiquitination
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
BRPI0514641A (pt) 2004-09-08 2008-06-17 Wisconsin Alumni Res Found cultivação das células tronco embriÈnica humana
EP1819809A4 (en) 2004-09-21 2008-08-06 Nvr Labs Ltd COMPOSITIONS AND METHODS FOR EXPANSION AND DIFFERENTIATION OF STEM CELLS
US8354277B2 (en) 2004-10-12 2013-01-15 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells derived from extended blastocysts
JP4646600B2 (ja) * 2004-11-02 2011-03-09 オリンパス株式会社 間葉系幹細胞の培養方法
US7641897B2 (en) 2004-11-23 2010-01-05 The Board Of Trustees Of The Leland Stanford Junior University Feeder layer and serum independent embryonic stem cells
FR2878860A1 (fr) 2004-12-08 2006-06-09 Vivalis Sa Lignees de cellules souches humaines derivees de cellules es et utilisations pour la production de vaccins et de proteines recombinantes
WO2006070370A2 (en) 2004-12-29 2006-07-06 Hadasit Medical Research Services & Development Limited Stem cells culture systems
US8597947B2 (en) 2004-12-29 2013-12-03 Hadasit Medical Research Services & Development Limited Undifferentiated stem cell culture systems
EP1853698A1 (en) 2005-01-28 2007-11-14 NovaThera Ltd. Methods for embryonic stem cell culture
US20060182724A1 (en) 2005-02-15 2006-08-17 Riordan Neil H Method for expansion of stem cells
US20090029461A1 (en) 2005-02-23 2009-01-29 Agency For Science Technology And Research Immortalised Feeder Cells
US8012751B2 (en) 2005-03-31 2011-09-06 Wisconsin Alumni Research Foundation Differentiation of pluripotent embryonic stem cells
JP4755460B2 (ja) * 2005-07-27 2011-08-24 オリンパス株式会社 間葉系幹細胞の培養方法
US7972767B2 (en) 2005-05-09 2011-07-05 Olympus Corporation Method for culturing mesenchymal stem cell and method for producing biological tissue prosthesis
US9446073B2 (en) 2005-05-15 2016-09-20 Biodontos, Llc Non-lineage committed precursor cells from the dental papillary tissue of teeth
US20110311977A1 (en) 2005-05-17 2011-12-22 Arundhati Mandal In Vitro Generation of Hepatocytes from Human Embryonic Stem Cells
CN101180389A (zh) 2005-05-17 2008-05-14 利莱恩斯生命科学有限公司 采用哺乳动物细胞确立人胚胎干细胞系
WO2007002210A2 (en) 2005-06-20 2007-01-04 Bresagen, Inc. Embryonic stem cell culture compositions and methods of use thereof
US9074181B2 (en) 2005-06-22 2015-07-07 Asterias Biotherapeutics, Inc. Suspension culture of human embryonic stem cells
AU2006269884A1 (en) * 2005-07-15 2007-01-25 Primegen Biotech, Llc Therapeutic reprogramming of germ line stem cells
WO2007026353A2 (en) 2005-08-29 2007-03-08 Technion Research & Development Foundation Ltd. Media for culturing stem cells
US7754483B2 (en) 2005-11-09 2010-07-13 The Penn State Research Foundation Systems and methods for selection and maintenance of homogeneous and pluripotent human embryonic stem cells
US20080070303A1 (en) 2005-11-21 2008-03-20 West Michael D Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby
EP2206724A1 (en) 2005-12-13 2010-07-14 Kyoto University Nuclear reprogramming factor
US20070249044A1 (en) 2006-01-30 2007-10-25 University Of Illinois At Chicago Microstructures in three dimensional gel suspensions for growth of cells
EP1983042B1 (en) 2006-01-31 2017-03-15 Daiichi Sankyo Company, Limited A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses
EP2420565B1 (en) * 2006-02-23 2017-08-30 ViaCyte, Inc. Compositions and methods useful for culturing differentiable cells
JP2007228815A (ja) 2006-02-27 2007-09-13 Gifu Univ 胚性幹細胞の維持方法
WO2007122233A1 (en) 2006-04-25 2007-11-01 Vrije Universiteit Brussel Preparation of mesenchymal progenitor cells, particularly osteogenic progenitor cells
US8241908B2 (en) 2006-05-16 2012-08-14 Becton, Dickinson And Company Extracellular matrix coated surface for culturing cells
US9096831B2 (en) 2006-05-19 2015-08-04 Versitech Limited Methods to enhance cell migration and engraftment
GB0613756D0 (en) 2006-07-12 2006-08-23 Univ Sheffield Cell culture medium
DK2733203T3 (da) 2006-08-02 2019-02-04 Technion Res & Dev Foundation Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur
WO2008033469A1 (en) 2006-09-15 2008-03-20 Children's Medical Center Corporation Methods for producing embryonic stem cells from parthenogenetic embryos
US20100166714A1 (en) 2006-11-02 2010-07-01 The General Hospital Corporation Cardiovascular stem cells, methods for stem cell isolation, and uses thereof
DK2081956T3 (da) 2006-11-13 2013-06-24 Charite Universitaetsmedizin FREMGANGSMÅDE TIL CELLEDYRKNING OG FREMGANGSMÅDE TIL BEHANDLING OMFATTENDE EN vEPO-PROTEINVARIANT
US8414928B2 (en) 2006-12-06 2013-04-09 George R. Dodge Methods for the generation of cartilage-like material by mechanical loading
WO2008075378A2 (en) 2006-12-19 2008-06-26 Stempeutics Research Private Limited Xeno-free culture conditions for human embryonic stem cells and methods thereof
US20080182328A1 (en) 2006-12-19 2008-07-31 The Burnham Institute Mammalian extraembryonic endoderm cells and methods of isolation
US10829733B2 (en) 2007-01-04 2020-11-10 Biolamina Ab Composition and method for enabling proliferation of pluripotent human stem cells
CN101605885B (zh) 2007-01-04 2013-03-27 卡尔·特吕格瓦松 能够使多能干细胞增殖的组合物及方法
SE0950586L (sv) 2007-01-17 2009-08-13 Wisconsin Alumni Res Found Förbättrad odling av stamceller
EP2108043A4 (en) 2007-01-18 2010-04-21 Suomen Punainen Risti Veripalv NEW METHODS AND REAGENTS FOR THE PRODUCTION OF CELLS
US20110033928A1 (en) 2007-03-09 2011-02-10 The Regents Of The University Of Michigan Methods and compositions for growth of cells and embryonic tissue on a synthetic polymer matrix
DE102007020302B4 (de) 2007-04-20 2012-03-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verbesserte dreidimensionale biokompatible Gerüststruktur, die Nanopartikel beinhaltet
US20090053182A1 (en) 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
FI20075417A0 (fi) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Koostumuksia ja menetelmiä alkion kantasolujen kasvatukseen
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
AU2008272949B2 (en) 2007-06-29 2014-05-15 FUJIFILM Cellular Dynamics, Inc. Automated method and apparatus for embryonic stem cell culture
EP2028268A1 (en) 2007-08-20 2009-02-25 Université Libre De Bruxelles Generation of neuronal cells from pluripotent stem cells
EP2191022A2 (en) 2007-08-21 2010-06-02 The Salk Institute for Biological Studies Robust regression based exon array protocol system and applications
US8623650B2 (en) 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
EP2072618A1 (en) 2007-12-14 2009-06-24 Johannes Gutenberg-Universität Mainz Use of RNA for reprogramming somatic cells
EP2242838A1 (en) 2007-12-17 2010-10-27 GliaMed, Inc. Stem-like cells and method for reprogramming adult mammalian somatic cells
WO2009099552A2 (en) 2008-01-30 2009-08-13 Corning Incorporated Cell culture article and screening
CN101939418A (zh) * 2008-01-30 2011-01-05 杰龙公司 用于培养干细胞衍生的心肌细胞的合成表面
KR20110046397A (ko) 2008-05-27 2011-05-04 오르후스 우니베르시테트 포유동물 줄기 세포 성장 및 분화를 위한 생체적합성 물질
US20100120142A1 (en) 2008-07-11 2010-05-13 Suomen Punainen Risti Veripalvelu Culture of human embryonic cells
US20100035327A1 (en) 2008-08-11 2010-02-11 Ann Marie Steele Use of rice-derived products in a universal cell culture medium
SG160248A1 (en) 2008-09-18 2010-04-29 Agency Science Tech & Res Use of novel monoclonal antibodies targeting human embryonic stem cells to characterize and kill induced pluripotent stem cells
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US8956867B2 (en) 2008-11-07 2015-02-17 Wisconsin Alumni Research Foundation Method for culturing stem cells
CA2753679C (en) 2009-02-27 2021-03-02 Cellular Dynamics International, Inc. Differentiation of pluripotent cells
WO2010129294A2 (en) 2009-04-27 2010-11-11 Schulz Thomas C Small molecules supporting pluripotent cell growth and methods thereof
US20120142103A1 (en) 2009-05-18 2012-06-07 Kohji Nishida Method for inducing differentiation into epithelial progenitor cell/stem cell population and corneal epithelial cell population from induced pluripotent stem cells
MX2011013385A (es) 2009-06-11 2012-05-29 Minerva Biotechnologies Corp Metodos para cultivar celulas madre y celulas progenitoras.
GB2483195B (en) 2009-06-12 2015-02-11 Nanyang Polytechnic Novel uses
KR101166257B1 (ko) 2009-06-16 2012-07-19 한국생명공학연구원 미분화 다능성줄기세포의 제작, 유지 및 증식을 위한 뉴로펩타이드 ｙ를 포함하는 배지 조성물 및 이를 이용한 다능성줄기세포의 배양 방법
WO2011019953A1 (en) 2009-08-12 2011-02-17 University Of Southern California Defined conditions for human embryonic stem cell culture and passage
EP3255142A1 (en) 2009-10-19 2017-12-13 Cellular Dynamics International, Inc. Cardiomyocyte production
WO2011058558A2 (en) 2009-11-12 2011-05-19 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
IL281453B (en) 2009-11-17 2022-07-01 Astellas Inst For Regenerative Medicine Methods for preparing human rpe cells and pharmaceutical preparations of human rpe cells
WO2011072399A1 (en) 2009-12-18 2011-06-23 The Governing Council Of The University Of Toronto Injectable polymer composition for use as a cell delivery vehicle
US8609416B2 (en) 2009-12-18 2013-12-17 Ventria Bioscience Methods and compositions comprising heat shock proteins
US20140030236A1 (en) 2010-02-25 2014-01-30 The Johns Hopkins University Derivation and maturation of synthetic and contractile vascular smooth muscle cells from human pluripotent stem cells
WO2011110886A1 (en) 2010-03-09 2011-09-15 Biolamina Ab Composition and method for enabling proliferation of pluripotent human stem cells
RU2012149102A (ru) 2010-04-20 2014-05-27 Интернэшнл Стем Селл Корпорейшн Физиологические способы выделения клеточных популяций высокой чистоты
US9725695B2 (en) 2010-05-05 2017-08-08 The Regents Of The University Of California Stem cell defined media for xeno-free and feeder free conditions and uses thereof
US11786636B2 (en) 2010-06-15 2023-10-17 Versitech Limited Methods for complex tissue engineering
AU2011268056B2 (en) 2010-06-18 2014-04-24 Cellular Dynamics International, Inc. Cardiomyocyte medium with dialyzed serum
EP3875599B1 (en) 2010-07-23 2024-12-11 Astellas Institute for Regenerative Medicine Methods for detection of rare subpopulations of cells and highly purified compositions of cells
US20120141432A1 (en) 2010-09-03 2012-06-07 University Of Pittsburgh- Of The Commonwealth System Of Higher Education Use of catalytic antioxidant to preserve stem cell phenotype and control cell differentiation
CA3150342A1 (en) 2010-09-07 2012-03-15 Technion Research & Development Foundation Limited Novel methods and culture media for culturing pluripotent stem cells
US8883502B2 (en) 2010-09-09 2014-11-11 The Regents Of The University Of California Expandable cell source of neuronal stem cell populations and methods for obtaining and using them

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