EE05591B1 - Meetod bioloogiliselt aktiivse tstokiini valmistamiseks ja bioloogiliselt aktiivse tstokiini muteerunud prekursorit kodeeriv cDNA - Google Patents

Meetod bioloogiliselt aktiivse tstokiini valmistamiseks ja bioloogiliselt aktiivse tstokiini muteerunud prekursorit kodeeriv cDNA

Info

Publication number
EE05591B1
EE05591B1 EEP200100530A EEP200100530A EE05591B1 EE 05591 B1 EE05591 B1 EE 05591B1 EE P200100530 A EEP200100530 A EE P200100530A EE P200100530 A EEP200100530 A EE P200100530A EE 05591 B1 EE05591 B1 EE 05591B1
Authority
EE
Estonia
Prior art keywords
toxin
biologically active
preparation
cdna encoding
mutated precursor
Prior art date
Application number
EEP200100530A
Other languages
English (en)
Estonian (et)
Inventor
Rubinstein Menachem
Liu Bianling
Novick Daniela
Graber Pierre
Original Assignee
Yeda Research And Development Co. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeda Research And Development Co. Ltd. filed Critical Yeda Research And Development Co. Ltd.
Publication of EE200100530A publication Critical patent/EE200100530A/xx
Publication of EE05591B1 publication Critical patent/EE05591B1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C12N9/6475—Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Steroid Compounds (AREA)
EEP200100530A 1999-04-13 2000-04-13 Meetod bioloogiliselt aktiivse tstokiini valmistamiseks ja bioloogiliselt aktiivse tstokiini muteerunud prekursorit kodeeriv cDNA EE05591B1 (et)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL12942799A IL129427A0 (en) 1999-04-13 1999-04-13 Preparation of biologically active molecules
PCT/IL2000/000220 WO2000061768A2 (en) 1999-04-13 2000-04-13 Preparation of biologically active molecules

Publications (2)

Publication Number Publication Date
EE200100530A EE200100530A (et) 2002-12-16
EE05591B1 true EE05591B1 (et) 2012-10-15

Family

ID=11072700

Family Applications (1)

Application Number Title Priority Date Filing Date
EEP200100530A EE05591B1 (et) 1999-04-13 2000-04-13 Meetod bioloogiliselt aktiivse tstokiini valmistamiseks ja bioloogiliselt aktiivse tstokiini muteerunud prekursorit kodeeriv cDNA

Country Status (23)

Country Link
US (1) US7098002B1 (de)
EP (1) EP1169460B1 (de)
JP (1) JP4822587B2 (de)
KR (1) KR100682185B1 (de)
CN (1) CN1324140C (de)
AR (1) AR023468A1 (de)
AT (1) ATE326536T1 (de)
AU (1) AU781342B2 (de)
BR (1) BR0009782A (de)
CA (1) CA2368994C (de)
DE (1) DE60028022T2 (de)
DK (1) DK1169460T3 (de)
EA (1) EA006825B1 (de)
EE (1) EE05591B1 (de)
ES (1) ES2259287T3 (de)
IL (3) IL129427A0 (de)
MX (1) MXPA01010487A (de)
NO (1) NO329525B1 (de)
PT (1) PT1169460E (de)
SI (1) SI1169460T1 (de)
UA (1) UA73940C2 (de)
WO (1) WO2000061768A2 (de)
ZA (1) ZA200108364B (de)

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WO2001029232A2 (en) * 1999-10-20 2001-04-26 Scios Inc. Functional cloning of genes encoding proteins/enzymes involved in proteolytic cleavage
US7273722B2 (en) * 2000-11-29 2007-09-25 Allergan, Inc. Neurotoxins with enhanced target specificity
WO2002101049A2 (en) 2001-03-08 2002-12-19 Ares Trading S.A. Interleukin-18 mutants, their production and use
IL142246A0 (en) * 2001-03-26 2002-03-10 Applied Research Systems Method and kit for quantitation of polypeptides
CA2475177A1 (en) 2002-02-11 2003-08-21 Zymogenetics, Inc. Materials and methods for preparing dimeric growth factors
ATE374825T1 (de) * 2003-02-21 2007-10-15 Biotecnol Sa Verwendung von caspasen zur herstellung von reifen rekombinanten fusionsproteinen
CA2534629C (en) * 2003-08-06 2016-01-19 University Of Maryland Biotechnology Institute Engineered proteases for affinity purification and processing of fusion proteins
CA2545024A1 (en) * 2003-11-24 2005-06-02 Novo Nordisk A/S Fusion proteins and methods of cleavage of such proteins
ATE517914T1 (de) 2004-03-08 2011-08-15 Zymogenetics Inc Dimere fusionsproteine und materialien und verfahren zu deren herstellung
ATE518882T1 (de) 2005-09-19 2011-08-15 Allergan Inc Mit clostridientoxin aktivierbare clostridientoxine
JP5093783B2 (ja) * 2006-04-20 2012-12-12 クリングルファーマ株式会社 Ｈｇｆ前駆体蛋白質改変体及びその活性型蛋白質
DE102007008596B4 (de) * 2007-02-15 2010-09-02 Friedrich-Schiller-Universität Jena Biologisch wirksame Moleküle auf Grundlage von PNA und siRNA, Verfahren zu deren zellspezifischen Aktivierung sowie Applikationskit zur Verabreichung
US20090304719A1 (en) 2007-08-22 2009-12-10 Patrick Daugherty Activatable binding polypeptides and methods of identification and use thereof
WO2009044988A1 (en) * 2007-10-01 2009-04-09 Korea Research Institute Of Bioscience And Biotechnology Method for large scale preparation of procaspases, which can be artificially activated, in e. coli expression system and their activation
DE102009043743B4 (de) 2009-03-13 2016-10-13 Friedrich-Schiller-Universität Jena Zellspezifisch wirksame Moleküle auf Grundlage von siRNA sowie Applikationskits zu deren Herstellung und Verwendung
RU2636046C2 (ru) 2009-01-12 2017-11-17 Сайтомкс Терапьютикс, Инк Композиции модифицированных антител, способы их получения и применения
AU2010215761B2 (en) 2009-02-23 2017-04-06 Cytomx Therapeutics, Inc Proproteins and methods of use thereof
CN103923897B (zh) * 2013-01-11 2016-06-01 中国人民解放军军事医学科学院生物工程研究所 caspase重组酶原及其编码基因与应用
CN103388001B (zh) * 2013-07-16 2016-01-20 中国人民解放军第四军医大学 一种含凝血酶切割位点gst膜型表达载体及转染阳性细胞的方法
RU2711322C1 (ru) 2013-12-20 2020-01-16 Ф. Хоффманн-Ля Рош Аг Улучшенные способы получения рекомбинантного полипептида
BR112017020690A2 (pt) 2015-03-30 2018-06-26 Greenlight Biosciences Inc produção livre de células de ácido ribonucleico
HUE057952T2 (hu) 2015-06-24 2022-06-28 Hoffmann La Roche Anti-transzferrin receptor antitestek testreszabott affinitással
TWI873952B (zh) 2015-10-02 2025-02-21 瑞士商赫孚孟拉羅股份公司 雙特異性抗‐人類cd20/人類轉鐵蛋白受體抗體及使用方法
AR106189A1 (es) 2015-10-02 2017-12-20 Hoffmann La Roche ANTICUERPOS BIESPECÍFICOS CONTRA EL A-b HUMANO Y EL RECEPTOR DE TRANSFERRINA HUMANO Y MÉTODOS DE USO
CA3020312A1 (en) 2016-04-06 2017-10-12 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid
KR102894284B1 (ko) 2017-10-11 2025-12-03 그린라이트 바이오사이언시스, 아이엔씨. 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물
KR20200080749A (ko) * 2018-12-27 2020-07-07 주식회사 폴루스 N-말단의 메티오닌이 절단된 목적 단백질 발현용 유전자 발현 카세트 및 이를 이용하여 n-말단의 메티오닌이 절단된 목적 단백질을 생산하는 방법
AU2020327671A1 (en) * 2019-08-13 2022-03-03 King's College London Immunoresponsive cells armoured with spatiotemporally restricted activity of cytokines of the IL-1 superfamily

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AU659412B2 (en) * 1990-06-25 1995-05-18 Osi Pharmaceuticals, Inc. Tissue-derived tumor growth inhibitors
DE69229252T2 (de) * 1991-08-16 1999-12-16 Merck & Co., Inc. DNS, welche das Interleukin-1B-Vorläufer-Converting-Enzym kodiert
AU683834B2 (en) * 1991-12-31 1997-11-27 Zymogenetics Inc. Methods for producing thrombin
JPH06125784A (ja) * 1992-01-17 1994-05-10 Takeda Chem Ind Ltd モノクローナル抗体，ハイブリドーマ，その製造法および用途
US6303369B1 (en) * 1993-09-30 2001-10-16 Carl Spana Protein expression system
EP0870028A1 (de) 1995-12-29 1998-10-14 Incyte Pharmaceuticals, Inc. Nukleinsäuren, die für interferon gamma-induzierenden faktor kodieren
JP4059941B2 (ja) * 1996-08-26 2008-03-12 社団法人芝蘭会 Ｒｈｏ標的タンパク質ｐ１４０ｍＤｉａ
AT405516B (de) * 1997-02-27 1999-09-27 Immuno Ag Faktor x-analoge mit modifizierter proteasespaltstelle
JPH10327872A (ja) * 1997-05-30 1998-12-15 Otsuka Pharmaceut Co Ltd Ｂｃｌ−２相互作用蛋白遺伝子ｂｉｓ
EP1013763B1 (de) * 1997-08-07 2011-02-02 Toray Industries, Inc. Methode zur herstellung von canine interleukin 18

Also Published As

Publication number Publication date
NO20014966L (no) 2001-12-11
CN1353761A (zh) 2002-06-12
EP1169460A2 (de) 2002-01-09
EE200100530A (et) 2002-12-16
NO20014966D0 (no) 2001-10-12
DE60028022T2 (de) 2006-09-28
AU781342B2 (en) 2005-05-19
WO2000061768A3 (en) 2001-02-08
EA006825B1 (ru) 2006-04-28
IL145842A0 (en) 2002-07-25
CN1324140C (zh) 2007-07-04
EP1169460B1 (de) 2006-05-17
DE60028022D1 (de) 2006-06-22
ES2259287T3 (es) 2006-10-01
BR0009782A (pt) 2002-01-08
IL129427A0 (en) 2000-02-17
DK1169460T3 (da) 2006-09-18
KR20020007365A (ko) 2002-01-26
AR023468A1 (es) 2002-09-04
JP4822587B2 (ja) 2011-11-24
MXPA01010487A (es) 2002-05-06
CA2368994A1 (en) 2000-10-19
CA2368994C (en) 2010-03-09
ATE326536T1 (de) 2006-06-15
PT1169460E (pt) 2006-07-31
JP2002541813A (ja) 2002-12-10
SI1169460T1 (sl) 2006-08-31
WO2000061768A2 (en) 2000-10-19
HK1044351A1 (en) 2002-10-18
IL145842A (en) 2008-06-05
UA73940C2 (uk) 2005-10-17
US7098002B1 (en) 2006-08-29
AU3985100A (en) 2000-11-14
KR100682185B1 (ko) 2007-02-12
NO329525B1 (no) 2010-11-01
ZA200108364B (en) 2002-10-11
EA200101078A1 (ru) 2002-04-25

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Effective date: 20170413