EE05591B1 - Meetod bioloogiliselt aktiivse tstokiini valmistamiseks ja bioloogiliselt aktiivse tstokiini muteerunud prekursorit kodeeriv cDNA - Google Patents

Meetod bioloogiliselt aktiivse tstokiini valmistamiseks ja bioloogiliselt aktiivse tstokiini muteerunud prekursorit kodeeriv cDNA

Info

Publication number
EE05591B1
EE05591B1 EEP200100530A EEP200100530A EE05591B1 EE 05591 B1 EE05591 B1 EE 05591B1 EE P200100530 A EEP200100530 A EE P200100530A EE P200100530 A EEP200100530 A EE P200100530A EE 05591 B1 EE05591 B1 EE 05591B1
Authority
EE
Estonia
Prior art keywords
toxin
biologically active
preparation
cdna encoding
mutated precursor
Prior art date
Application number
EEP200100530A
Other languages
English (en)
Estonian (et)
Inventor
Rubinstein Menachem
Liu Bianling
Novick Daniela
Graber Pierre
Original Assignee
Yeda Research And Development Co. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeda Research And Development Co. Ltd. filed Critical Yeda Research And Development Co. Ltd.
Publication of EE200100530A publication Critical patent/EE200100530A/xx
Publication of EE05591B1 publication Critical patent/EE05591B1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C12N9/6475—Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Steroid Compounds (AREA)
EEP200100530A 1999-04-13 2000-04-13 Meetod bioloogiliselt aktiivse tstokiini valmistamiseks ja bioloogiliselt aktiivse tstokiini muteerunud prekursorit kodeeriv cDNA EE05591B1 (et)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL12942799A IL129427A0 (en) 1999-04-13 1999-04-13 Preparation of biologically active molecules
PCT/IL2000/000220 WO2000061768A2 (en) 1999-04-13 2000-04-13 Preparation of biologically active molecules

Publications (2)

Publication Number Publication Date
EE200100530A EE200100530A (et) 2002-12-16
EE05591B1 true EE05591B1 (et) 2012-10-15

Family

ID=11072700

Family Applications (1)

Application Number Title Priority Date Filing Date
EEP200100530A EE05591B1 (et) 1999-04-13 2000-04-13 Meetod bioloogiliselt aktiivse tstokiini valmistamiseks ja bioloogiliselt aktiivse tstokiini muteerunud prekursorit kodeeriv cDNA

Country Status (23)

Country Link
US (1) US7098002B1 (de)
EP (1) EP1169460B1 (de)
JP (1) JP4822587B2 (de)
KR (1) KR100682185B1 (de)
CN (1) CN1324140C (de)
AR (1) AR023468A1 (de)
AT (1) ATE326536T1 (de)
AU (1) AU781342B2 (de)
BR (1) BR0009782A (de)
CA (1) CA2368994C (de)
DE (1) DE60028022T2 (de)
DK (1) DK1169460T3 (de)
EA (1) EA006825B1 (de)
EE (1) EE05591B1 (de)
ES (1) ES2259287T3 (de)
IL (3) IL129427A0 (de)
MX (1) MXPA01010487A (de)
NO (1) NO329525B1 (de)
PT (1) PT1169460E (de)
SI (1) SI1169460T1 (de)
UA (1) UA73940C2 (de)
WO (1) WO2000061768A2 (de)
ZA (1) ZA200108364B (de)

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WO2001029232A2 (en) * 1999-10-20 2001-04-26 Scios Inc. Functional cloning of genes encoding proteins/enzymes involved in proteolytic cleavage
US7273722B2 (en) 2000-11-29 2007-09-25 Allergan, Inc. Neurotoxins with enhanced target specificity
ATE315647T1 (de) 2001-03-08 2006-02-15 Ares Trading Sa Interleukin -18 mutantenproteine, deren herstellung und verwendung
IL142246A0 (en) * 2001-03-26 2002-03-10 Applied Research Systems Method and kit for quantitation of polypeptides
AU2003210996A1 (en) 2002-02-11 2003-09-04 Zymogenetics, Inc. Materials and methods for preparing dimeric growth factors
NZ541551A (en) * 2003-02-21 2007-02-23 Biotecnol Sa Use of caspase enzymes for maturation of engineered recombinant polypeptide fusions
CA2534629C (en) * 2003-08-06 2016-01-19 University Of Maryland Biotechnology Institute Engineered proteases for affinity purification and processing of fusion proteins
JP2007512008A (ja) * 2003-11-24 2007-05-17 ノボ　ノルディスク　アクティーゼルスカブ 融合タンパク質及び該タンパク質の切断方法
ATE517914T1 (de) 2004-03-08 2011-08-15 Zymogenetics Inc Dimere fusionsproteine und materialien und verfahren zu deren herstellung
ES2369558T3 (es) * 2005-09-19 2011-12-01 Allergan, Inc. Toxinas clostridiales y toxinas clostridiales activables.
EP2014676B1 (de) * 2006-04-20 2012-03-07 Kringle Pharma Inc. Mutante des hgf-precursor-proteins und aktivierte form davon
DE102007008596B4 (de) * 2007-02-15 2010-09-02 Friedrich-Schiller-Universität Jena Biologisch wirksame Moleküle auf Grundlage von PNA und siRNA, Verfahren zu deren zellspezifischen Aktivierung sowie Applikationskit zur Verabreichung
CA2697032C (en) * 2007-08-22 2021-09-14 The Regents Of The University Of California Activatable binding polypeptides and methods of identification and use thereof
KR100980315B1 (ko) * 2007-10-01 2010-09-07 한국생명공학연구원 대장균시스템을 이용한 인위적 활성화가 가능한 카스파제전구체 대량생산 및 활성화 방법
DE102009043743B4 (de) 2009-03-13 2016-10-13 Friedrich-Schiller-Universität Jena Zellspezifisch wirksame Moleküle auf Grundlage von siRNA sowie Applikationskits zu deren Herstellung und Verwendung
US20100189651A1 (en) * 2009-01-12 2010-07-29 Cytomx Therapeutics, Llc Modified antibody compositions, methods of making and using thereof
JP5861223B2 (ja) * 2009-02-23 2016-02-16 サイトムエックス セラピューティクス, インク.CytomX Therapeutics, Inc. プロタンパク質およびその使用方法
CN103923897B (zh) * 2013-01-11 2016-06-01 中国人民解放军军事医学科学院生物工程研究所 caspase重组酶原及其编码基因与应用
CN103388001B (zh) * 2013-07-16 2016-01-20 中国人民解放军第四军医大学 一种含凝血酶切割位点gst膜型表达载体及转染阳性细胞的方法
JP6618912B2 (ja) 2013-12-20 2019-12-11 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改良された、組換えポリペプチド作製方法
KR20180002636A (ko) 2015-03-30 2018-01-08 그린라이트 바이오사이언시스, 아이엔씨. 리보핵산의 무세포 생산
MX391086B (es) 2015-06-24 2025-03-21 Hoffmann La Roche Anticuerpos anti-receptor de transferrina con afinidad diseñada.
NZ741067A (en) 2015-10-02 2023-07-28 Hoffmann La Roche Bispecific anti-human cd20/human transferrin receptor antibodies and methods of use
AR106189A1 (es) 2015-10-02 2017-12-20 Hoffmann La Roche ANTICUERPOS BIESPECÍFICOS CONTRA EL A-b HUMANO Y EL RECEPTOR DE TRANSFERRINA HUMANO Y MÉTODOS DE USO
CR20180525A (es) 2016-04-06 2019-02-14 Greenlight Biosciences Inc Producción de ácido ribonucleico libre de células
BR112020007068A2 (pt) 2017-10-11 2020-10-06 Greenlight Biosciences, Inc. métodos e composições para produção de nucleosídeo trifosfato e ácido ribonucleico
KR20200080749A (ko) * 2018-12-27 2020-07-07 주식회사 폴루스 N-말단의 메티오닌이 절단된 목적 단백질 발현용 유전자 발현 카세트 및 이를 이용하여 n-말단의 메티오닌이 절단된 목적 단백질을 생산하는 방법
EP4013857A1 (de) * 2019-08-13 2022-06-22 King's College London Immunreaktive zellen, die mit räumlich eingeschränkter aktivität von cytokinen der il-1-superfamilie gepanzert sind

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JPH05509320A (ja) * 1990-06-25 1993-12-22 オーエスアイ　ファーマシューティカルズ，インコーポレイティド 組織誘導型腫瘍成長抑制物質、その調製及び使用方法
US5824297A (en) * 1990-06-25 1998-10-20 Oncogene Science, Inc. Tissue-derived tumor growth inhibitors, methods of preparation and uses thereof
DE69229252T2 (de) * 1991-08-16 1999-12-16 Merck & Co., Inc. DNS, welche das Interleukin-1B-Vorläufer-Converting-Enzym kodiert
EP0624199B1 (de) * 1991-12-31 1999-05-06 Zymogenetics, Inc. Verfahren zur Herstellung von Thrombin
JPH06125784A (ja) * 1992-01-17 1994-05-10 Takeda Chem Ind Ltd モノクローナル抗体，ハイブリドーマ，その製造法および用途
US6303369B1 (en) * 1993-09-30 2001-10-16 Carl Spana Protein expression system
WO1997024441A1 (en) * 1995-12-29 1997-07-10 Incyte Pharmaceuticals, Inc. Nucleic acids encoding interferon gamma inducing factor-2
JP4059941B2 (ja) * 1996-08-26 2008-03-12 社団法人芝蘭会 Ｒｈｏ標的タンパク質ｐ１４０ｍＤｉａ
AT405516B (de) 1997-02-27 1999-09-27 Immuno Ag Faktor x-analoge mit modifizierter proteasespaltstelle
JPH10327872A (ja) * 1997-05-30 1998-12-15 Otsuka Pharmaceut Co Ltd Ｂｃｌ−２相互作用蛋白遺伝子ｂｉｓ
US6600020B1 (en) * 1997-08-07 2003-07-29 Toray Industries, Inc. Canine interleukin 18 polypeptide

Also Published As

Publication number Publication date
NO20014966D0 (no) 2001-10-12
EA200101078A1 (ru) 2002-04-25
PT1169460E (pt) 2006-07-31
IL145842A0 (en) 2002-07-25
WO2000061768A3 (en) 2001-02-08
BR0009782A (pt) 2002-01-08
EA006825B1 (ru) 2006-04-28
DK1169460T3 (da) 2006-09-18
EP1169460A2 (de) 2002-01-09
DE60028022T2 (de) 2006-09-28
AU3985100A (en) 2000-11-14
CN1353761A (zh) 2002-06-12
US7098002B1 (en) 2006-08-29
KR20020007365A (ko) 2002-01-26
SI1169460T1 (sl) 2006-08-31
IL129427A0 (en) 2000-02-17
ZA200108364B (en) 2002-10-11
CA2368994A1 (en) 2000-10-19
DE60028022D1 (de) 2006-06-22
CA2368994C (en) 2010-03-09
NO329525B1 (no) 2010-11-01
MXPA01010487A (es) 2002-05-06
EE200100530A (et) 2002-12-16
AR023468A1 (es) 2002-09-04
JP4822587B2 (ja) 2011-11-24
HK1044351A1 (en) 2002-10-18
CN1324140C (zh) 2007-07-04
AU781342B2 (en) 2005-05-19
IL145842A (en) 2008-06-05
ATE326536T1 (de) 2006-06-15
JP2002541813A (ja) 2002-12-10
WO2000061768A2 (en) 2000-10-19
ES2259287T3 (es) 2006-10-01
KR100682185B1 (ko) 2007-02-12
EP1169460B1 (de) 2006-05-17
UA73940C2 (uk) 2005-10-17
NO20014966L (no) 2001-12-11

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Effective date: 20170413