EP0346400A1 - Traitement de tumeurs malignes avec du phosphate 8-chloroadenosine 3',5'-cyclique, du phosphate 8-aminoadenosine 3',5'-cyclique et leur preparation - Google Patents

Traitement de tumeurs malignes avec du phosphate 8-chloroadenosine 3',5'-cyclique, du phosphate 8-aminoadenosine 3',5'-cyclique et leur preparation

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Publication number
EP0346400A1
EP0346400A1 EP88905052A EP88905052A EP0346400A1 EP 0346400 A1 EP0346400 A1 EP 0346400A1 EP 88905052 A EP88905052 A EP 88905052A EP 88905052 A EP88905052 A EP 88905052A EP 0346400 A1 EP0346400 A1 EP 0346400A1
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Prior art keywords
cyclic phosphate
chloroadenosine
day
aminoadenosine
tumor
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EP88905052A
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German (de)
English (en)
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EP0346400A4 (en
Inventor
Roland K. Robins
Ganapathi R. Revankar
Yu-An Chang
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Nucleic Acid Research Institute
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Nucleic Acid Research Institute
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Priority claimed from US07/136,407 external-priority patent/US4861873A/en
Application filed by Nucleic Acid Research Institute filed Critical Nucleic Acid Research Institute
Publication of EP0346400A1 publication Critical patent/EP0346400A1/fr
Publication of EP0346400A4 publication Critical patent/EP0346400A4/en
Withdrawn legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
    • A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
    • A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
    • A61K31/7076—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids

Definitions

  • TITLE TREATMENT OF MALIGNANT TUMORS WITH 8-CHLOROADENOSINE 3',5'-cyclic PHOSPHATE, 8-AMINOADENOSINE 3',5'-cyclic PHOSPHATE AND PREPARATION THEREOF
  • This invention is directed to treating malignant tumors in vivo utilizing the compounds 8-chloroadenosine 3',5'-cyclic phosphate and/or 8-aminoadenosine 3',5'-cyclic phosphate.
  • the compound 8-chloroadenosine 3',5'-cyclic phosphate and other related adenine and adenosine compounds are prepared by two novel syntheses utilizing hydrogen chloride and m -chlo r ope roxybenz o ic ac i d in a su itabl e s olvent in the f i r st synthesis and N-chlorosuccinamide and acetic acid in a suitable solvent in the second synthesis.
  • cAMP functions by binding to a cAMP receptor protein which has two different cAMP binding sites.
  • the cAMP receptor protein is a regulatory subunit of a cAMP dependent protein kinase. There apparently is site selectivity in binding to one or the other of two sites. This activity can thus be described as site 1-selectivity and site 2-selectivity.
  • 8- chloroadenosine 3',5'-cyclic phosphate hereinafter alternately also identified as 8-chloro cAMP
  • 8-aminoadenosine 3',5'-cyclic phosphate hereinafter alternately also identified as 8- amino cAMP
  • two novel preparative syntheses yield 8-chloroadenosine compounds such as 8-chloroadenosine 3',5'-cyclic phosphate directly from respective adenosine precursors.
  • the present invention relates to the use of 8-chloroadenosine 3',5'-cyclic phosphate (8-chloro cAMP) and 8- aminoadenosine 3',5'-cyclic phosphate (8-amino cAMP) together or separately in treating malignant tumors in warm blooded animals.
  • the antitumor properties of 8-chloroadenosine 3',5'-cyclic phosphate and 8- aminoadenosine 3',5'-cyclic phosphate are achieved by administering to a warm blooded animal an effective amount of a pharmaceutical composition containing 8-chloroadenosine 3',5'- cyclic phosphate or 8-aminoadenosine 3',5'-cyclic phosphate, together or separately, or pharmaceutically acceptable salts thereof as active compounds in at least 0.1% by weight based on the total weight of the composition.
  • a pharmaceutical carrier would be utilized.
  • the carrier would be chosen to allow for administration of a suitable concentration of 8-chloroadenosine 3',5'-cyclic phosphate and/or 8-aminoadenosine 3',5'-cyclic phosphate either by oral administration, ophthalmic administration, topical administration, suppository administration or by suitable injection as a solution or suspension into the affected warm blooded animal.
  • 8-chloroadenosine 3',5'-cyclic phosphate and/or 8-aminoadenosine 3 ',5'-cyclic phosphate of the invention would be dependent upon the host harboring the malignant tumor, the type of tumor and the tumor site.
  • 8-chloroadenosine 3',5'-cyclic phosphate and/or 8-aminoadenosine 3',5'-cyclic phosphate of the invention could be administered intraveneously, intramuscularly, intracerebrally, subcutaneously or intraperitoneally.
  • a physiologically accepted salt as for instance the sodium, potassium or ammonium salt, could be used.
  • a physiologically accepted salt as for instance the sodium, potassium or ammonium salt.
  • the invention includes improved processes for the preparation of 8-chloro derivatives of adenine, adenosine, adenosine 5'-monophosphate and adenosine 3',5'-cyclic phosphate and related compounds.
  • 8-chloroadenine, 8-chloroadenosine, 8-chloroadenosine 5'-monophosphate and 8-chloroadenosine 3',5'-cyclic phosphate or other related adenosine compounds are prepared directly from corresponding respective adenosine precursor compounds by processes for preparing chloro compounds of the formula
  • R is H or
  • R 1 and R 2 are H or or together R 1 and R 2 are and R 3 and R 4 are H or one of R 3 or R 4 is OH and the other is
  • R 1 and R 2 are H or or together R 1 and R 2 are and R 3 and R 4 are H or one of R 3 or R 4 is OH and the other is
  • 8-Aminoadenosine 3',5'-cyclic phosphate of the invention is prepared as is described in the above referenced paper, K. Mumeyama et al. Biochemistry, 10, 2390, 1971.
  • 8-Chloroadenosine 3',5'-cyclic phosphate of the invention can be prepared as is described in above referenced paper, K. Muneyama et al, J. Carbohydr.
  • Nucleosides Nucleotides, 1, 55 1974, via an 8-bromo intermediate compound, however, by utilizing new and improved processes as discussed herein, 8-chloroadenosine 3',5'-cyclic phosphate and related compounds are obtained directly from respective adenosine precursors avoiding having to synthesis intermediate compounds. As such, improvements in the economy of the synthesis are obtained.
  • adenosine 3',5'-cyclic phosphate is directly chlorinated to 8-chloroadenosine 3',5'-cyclic phosphate utilizing N-chlorosuccinimide and a suitable weak acid in a suitable solvent.
  • Suitable as the weak acid is acetic acid. Alternatively formic acid or other weak organic acid might be used. In choosing the acid, consideration is given whereby the acid is of such a strength that it is sufficiently weak so as not to cleave the sugar - heterocycle glycosidic bond (the C1'-N9 bond).
  • Suitable as the solvent are dimethylacetamide, dimethylf ormamide or aqueous medium.
  • adenosine 3',5'- cyclic phosphate is also directly chlorinated to 8-chloroadenosine 3',5'-cyclic phosphate utilizing anhydrous hydrogen chloride and a suitable oxidizing agent such as m-chloroperoxybenzoic acid.
  • This reaction is conduct edin a suitable solvent as for example dimethylacetamide or dimethylf ormamide.
  • oxidizing agent sodium hypochlorite can be mentioned.
  • both of these processes can be utilized to prepare 8-chloroadenosine from adenosine or 8-chloroadenosine 5'-phosphate from adenosine 5'-phosphate.
  • the hydrogen chlor ide/m-chloroperoxybenzoic acid reaction is carried out in the presence of moisture, it can be utilized to prepare 8-chloroadenine from adenosine.
  • these two novel processes might also be utilized to convert suitable derivatives of adenosine, adenosine 5'-phosphate, adenosine 3'-phosphate and adenosine 3',5'-cyclic phosphate, to their respective 8-chloro derivatives.
  • Such derivatives would include the 2-deoxy- ⁇ -D-erythro-pent of uranosyl sugar derivative and the 2- ⁇ -D-arabinofuranosyl sugar derivative and might also include substituents on the purine amino group, the 2 position of the purine ring, sugar hydroxyl groups or even other heterocyclic moieties such as a deaza purine or the like.
  • an adenosine compound e.g. adenosine, AMP or cAMP
  • adensoine yields compounds 1 and 2
  • AMP yields compound 3
  • cAMP yields compound 4. This is achieved using one, the other or both of the two novel processes.
  • One process uses hydrogen chloride and ni-ohloroperoxybenzoic acid in an appropiate solvent such as dimethylacetamide or dimethylf oramide.
  • the other process uses N-chlorosuccinamide and acetic acid in an appropriate solvent such as dimethylacetamide, dimethylf ormamide or an aqueous acetic acid.
  • Any of the 8-chloro compounds thus formed can then be further converted in a further steo to their 8- chloroinosine analogs as for instance the conversion of 8-chloroadenosine 3',5'-cyclic phosphate, compound 4, to 8-chloroinosine 3',5'-cyclic phosphate, compound 5.
  • adenosine (2.67 g, 10 mmol) in DMA/HCl (0.5 M, 45 mL) was added m-chloroperoxybenzoic acid (MCPBA, 3.22 g, 16 mmol, 87%) and stirred at room temperature for 2.5 h. An additional portion of MCPBA (0.9 g, 5 mmol) was added and stirring continued for another 1 h. Toluene (50 mL) was added to the reaction mixture and the solvents evaporated at 60°C under vacuo to dryness. The residue was dissolved in water (50 mL) and extracted with ether (3 ⁇ 50 mL).
  • MCPBA m-chloroperoxybenzoic acid
  • N-Chlorosuccinamide N-Chlorosuccinamide
  • Adenosine 5'-monophosphate monohydrate (3.11 g, 8.5 mmol), NaCl (3.11 g, 53 mmol) and NCS (3.33 g, 25 mmol) were dissolved in 50% aqueous AcOH (100 mL) and the solution was stirred at room tempeature for 5 days. Evaporation of the reaction mixture and purification of the residue by HPLC on a C-18 reverse phase column using 0.5% aqueous AcOH gave 2.30 g (60%) of the title compound, which -&ks identical to 3 prepared by Method B.
  • EXAMPLE 5 8-Chloroinosine 3',5'-cyclic phosphate (5). To a suspension of 8-chloroadenosine 3',5'-cyclic phosphate (4, 1.0 g, 2.7 mmol) in H 2 O (3 mL) was added 2N NaOH dropwise until a clear solution was obtained. NaN0 2 (1.05 g, 15 mmol) was added to the reaction solution, followed by dropwise addition of AcOH (2 mL). The reaction mixture was stirred at room temperature overnight and then evaporated to dryness under reduced pressure at 35°C. The residue was purified on a Dowex 50 ⁇ 2 - 200 (H + ) resin column (5 ⁇ 20 cm) eluting with H 2 O.
  • Acceptable salts of the phosphate moiety can be selected from, but not necessarily limited to the group consisting of alkali and alkaline earths, e.g. sodium, potassium, calcium, magnesium, lithium, or ammonium and substituted ammonium, trialklyammonium, dialkylammonium, alkylammonium, e.g. triethylammonium, trimethylammonium, diethylammonium, octylammonium, cetyltrimethylammonium and cetylpridium.
  • alkali and alkaline earths e.g. sodium, potassium, calcium, magnesium, lithium, or ammonium and substituted ammonium, trialklyammonium, dialkylammonium, alkylammonium, e.g. triethylammonium, trimethylammonium, diethylammonium, octylammonium, cetyltrimethylammonium and cetylpridium.
  • Such a salt would
  • 8-chloroadenosine 3',5'-cyclic phosphate and/or 8-aminoadenosine 3',5'-cyclic phosphate, as. free acids or as salts are appropriately mixed with a suitable pharmaceutical carrier which, since the compounds of the invention are water soluble, may be as simple as sterilized water or could be a complex carrier having appropriate agents to suitably mimic certain biological environmental, i.e., pH or salt adjusted for solution suitable for intravenous, intramuscular or other injections.
  • 8-Chloroadenosine 3',5'-cyclic phosphate and/or 8-aminoadenosine 3',5'- cyclic phosphate might be appropriately used in the presence of a suitable buffer or as a salt as discussed above.
  • the compounds of the invention are especially useful in treating carcinoma. Included in such a class are mammary, colon, bladder, lung, prostate, stomach and pancreas carcinoma.
  • the treatment method is effective in bringing about regression, palliation, inhibition of growth, and remission of tumors.
  • 8-chloroadenosine 3',5'-cyclic phosphate and/or 8-aminoadenosine 3',5'-cyclic phosphate of the invention or salts thereof would be mixed with an appropriate pharmaceutical carrier such that 8-chloroadenosine 3',5'-cyclic phosphate and/or 8-aminoadenosine 3',5'-cyclic phosphate would be suitably soluble in the carrier.
  • suspensions, emulsions and other formulations of 8-chloroadenosine 3',5'-cyclic phosphate and 8-aminoadenosine 3',5'-cyclic phosphate of the invention could be used where indicated.
  • the pharmaceutical carrier in addition to naving a solubilizing or suspending agent therein, might also include suitable dilutants, buffers, surface active agents and other similar agents as are typically used in pharmaceutical carriers.
  • the total composition of the pharmaceutical carrier would, however, be chosen to be compatible with the site of delivery, the concentration of the active ingredient and other parameters as are standard in pharmaceutical industry.
  • 8-Chloroadenosine 3',5'-cyclic phosphate and/or 8-aminoadenosine 3',5'-cyclic phosphate of the invention would be suitably admixed with the pharmaceutical carrier such that they would be present in a concentration of at least 0.1 percent by weight of the total composition. Preferredly, they would be present in the pharmaceutical carrier at a concentration of about 10% to about 90% by weight of the total composition.
  • effective amounts of 8-chloroadenosine 3',5'-cyclic phosphate typically would range from about 13 milligrams per kilogram per day (mg/kg/day) of the total body weight of the treated warm blooded animal to about 288 mg/kg/day. Preferredly, this range would be from 22 mg/kg to about 173 mg/kg/day. Based on present studies, effective amounts of 8-aminoadenosine 3',5'-cyclic phosphate typically would range from about 13 milligrams per kilogram per day (mg/kg/day) of the total body weight of the treated warm blooded animal to about 104 mg/kg/day.
  • this range would be from 13 mg/kg to about 37 mg/kg/day.
  • the amounts of 8-chloroadenosine 3',5'-cyclic phosphate and 8-aminoadenosine 3',5'-cyclic phosphate utilized in treating an afflicted animal would take into account parameters such as the type of tumor, the tumor site, the form of administering and the physical size and condition of the host. In any event, the actual amount should be sufficient to provide a chemotherapeutically effective amount of the agent in the host in a convenient volume, which will be readily within the ability of those skilled in the art to determine given the disclosure herein.
  • 8-Chloroadenosine 3',5'-cyclic phosphate and 8-aminoadenosine 3',5'-cyclic phosphate can be administered together admixed with a suitable pharmaceutical carrier.
  • a suitable pharmaceutical carrier As is evident from the examples and tables below both of these compounds when administered independently to a host exhibit certain antitumor activity. When both of the compounds are administered together to a host the antitumor activity is maintained and the compounds are not antagonistic to one another. Further, while we do not wish to be bound by theory, at this time it is believed that when administered together the compounds 8-chloroadenosine 3',5'-cyclic phosphate and 8- aminoadenosine 3',5'-cyclic phosphate exhibit synergistic activity.
  • Singularly 8-chloroadenosine 3',5'-cyclic phosphate exhibits a very broad spectrum of activity against many different tumors.
  • Singularly 8-aminoadenosine 3',5'-cyclic phosphate exhibits significant activity against colon carcinoma.
  • the combination of the compounds 8-chloroadenosine 3 ',5'-cyclic phosphate and 8-aminoadenosine 3',5'-cyclic phosphate also exhibits significant activity against colon carcinoma, however, the activity of the combination of 8-chloroadenosine 3',5'-cyclic phosphate and 8-aminoadenosine 3',5'-cyclic phosphate is greater than the activity of 8-chloroadenosine 3',5'-cyclic phosphate when used alone.
  • the activity is enhanced compared to 8-chloroadenosine 3',5'-cyclic phosphate when used independently. Further this enhanced activity is maintained beyond a post treatment period. Additionally with the combination of the two compounds 8- chloroadenosine 3',5'-cyclic phosphate and 8-aminoadenosine 3',5'-cyclic phosphate there is decreased drug rebound after discontinuous of the drug.
  • the 8-chloroadenosine 3',5'-cyclic phosphate and/or 8-aminoadenosine 3',5'-cyclic phosphate of the invention can be given as single doses or as multiple doses divided into sub- doses given daily or over a period of days.
  • 8-chloroadenosine 3',5'-cyclic phosphate and 8-aminoadenosine 3',5'-cyclic phosphate of the invention exhibits certain inhanced responses when administered as an infusion and, as such, this will be taken in to account in the optimization of a dosage schedule as is well within the skill of the Art given the disclosure herein.
  • test results are indicated as %T/C.
  • %T/C a value greater than 125% is considered as having statically meaningful activity.
  • test results are given as change in mean tumor weight. This is expressed in two ways. If there was an increase in tumor weight the results are expressed as ⁇ T/ ⁇ C. However, if there was a net negative change in tumor weight the results are expressed as ⁇ T/T.
  • the activity of 8-chloroaden ⁇ sine 3',5'-cyclic phosphate against L1210 inoculated BDF 1 mice was determined by both bolus injection and by infusion. As shown in Table 2 below, when delivered by bolus injection there was insignificant activity, however, when infused into a test animal in a dose range of from 22 mg to 173 mg/kg/day, 8-chloroadenosine 3',5'-cyclic phosphate exhibited significant antitumor activity. Further, the toxicity was determined for the infusion test animals.
  • mice were inoculated i.p. with 1 ⁇ 10 6 cells of murine leukemia L1210 24-hr before first treatment. Each treatment group consisted of 5 mice. Twenty control mice that received a 0.9% solution of NaCl lived 6.2 ⁇ days.
  • Tumor Weight (mg/kg/day) Weight (mg) Tumor Weight 2 Tumor Weight ( ⁇ T/ ⁇ C or ⁇ T/T) - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 7 days was started, each treatment group consisted of 7 mice.
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 7 days was started, each treatment group consisted of 7 mice.
  • 8-chloroadenosine 3',5'-cyclic phosphate was further tested in athymic mice against human mammary carcinoma MDA-MB- 231. The test was also done using infusion as the route of administration. The results of this test are shown in Table 5a below. The compound exhibited significant activity at the dose levels of 62 and 104 mg/kg/day. Further while it did not exhibit significant activity at all dose levels it did show a linearity of response throughout the tested dosage range against this tumor line. Table 5a. Influence of intraperitoneally infused 8-Cl-cAMP Na + on the growth in athymic mice of human mammary carcinoma
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 7 days was started, each treatment group consisted of 7 mice.
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 5 days was started, each treatment group consisted of 7 mice.
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 7 days was started, each treatment group consisted of 7 mice.
  • 8-Aminoadenosine 3',5'-cyclic phosphate as a sodium salt was tested utilizing nontumor bearing BDF 1 mice to establish a lethal toxicity for the drug.
  • the drug was delivered i.p. by a bolus injection given as a single dose on day one.
  • Table 8 As is evident from Table 8 below at 104 mg/kg per injection there were no toxic deaths. At the higher levels of 173 mg/kg and 288 mg/kg there was lethal toxicity for 1/5 and 5/5 mice respectively.
  • 8-aminoadenosine 3',5'-cyclic phosphate was delivered 24 hr/day for 5 consecutive days by i.p. infusion, 62 mg/kg was the maximum tolerated dose and at a dose of 104 mg/kg or more the compound was lethally toxic for 13/13 mice.
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 7 days was started, each treatment group consisted of 7 mice.
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 6 days was started, each treatment group consisted of 6 mice.
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 7 days was started, each treatment group consisted of 6 mice.
  • 8-Aminoadenosine 3',5'-cyclic phosphate was further tested in athymic mice against human lung carcinoma LX-1. This test was further done by infusion and with treatment over a period of five days. The results of this test are shown in Table 12 below .
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 5 days was started, each treatment group consisted of 6 mice.
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 7 days was started, each treatment group consisted of 6 mice.
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-I female mice. Three weeks later the tumors were staged and treatment lasting 7 days was started, each treatment group consisted of 7 mice.
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 7 days was started, each treatment group consisted of 6 mice.
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 7 days was started, each treatment group consisted of 6 mice.
  • 8-chloroadenosine 3',5'-cyclic phosphate and 8-aminoadenosine 3',5'-cyclic phosphate are administered together in athymic mice against human colon carcinoma HT-29.
  • This combination drug therapy was compared to administration of 8-chloroadenosine 3',5'-cyclic phosphate as an independent drug at the same dose level as utilized in the combination therapy. Both the combination drug and the single drug therapy were given by infusion with treatment lasting for seven days.
  • 8-aminoadenosine 3',5'-cyclic phosphate and 8-chloroadenosine 3',5'-cyclic phosphate might be synergistic in their action as antitumor agents.
  • Tumor fragments ( ⁇ 14 mg) were implanted subcutaneously in the thigh region of athymic CD-1 female mice. Three weeks later the tumors were staged and treatment lasting 7 days was started, each treatment group consisted of 7 mice.
  • ⁇ T/T was calculated in lieu of ⁇ T/ ⁇ C.
  • treatment was effected up to and including day seven.
  • the test animals how- ⁇ fer were followed to day 21.
  • On day 12 in the animals treated with the combination of 8-aminoadenosine 3',5'-cyclic phosphate and 8-chloroadenosine 3',5'-cyclic phosphate tumor weight was still less than that of the controlled animals on day 1, that is tumor weight at day 12 for the combination of the two compounds was still less than the initial tumor weight at day one.
  • 8-chloroadenosine 3',5'-cyclic phosphate exhibits a broad spectrum of activity against many human tumor cells
  • 8-Aminoadenosine 3',5'-cyclic phosphate exhibits good specificity against human colon tumors and the combination of 8-aminoadenosine 3',5'-cyclic phosphate and 8-chloroadenosine 3',5'-cyclic phosphate is not antagonistic.
  • 8-chloroadenosine 3',5'-cyclic phosphate and 8-aminoadenosine 3',5'-cyclic phosphate of the invention can be formulated in various formulations to prepare pharmaceutical compositions containing 8-chloroadenosine 3',5'-cyclic phosphate, 8-Aminoadenosine 3',5'-cyclic phosphate or a combination of 8-chloroadenosine 3',5'-cyclic phosphate and 8-aminoadenosine 3',5'-cyclic phosphate the invention as active ingredients.
  • Pharmaceutical Preparative Example 22 illustrates the use of 8-chloroadenosine 3',5'-cyclic phosphate sodium salt and/or 8-aminoadenosine 3',5'-cyclic phosphate sodium salt in injectables suitable for intravenous or other types of injection into the host animal.
  • Pharmaceutical Preparative Example 23 is directed to an oral syrup preparation, Pharmaceutical Preparative Example 24 to an oral capsule preparation and Pharmaceutical Preparative Example 25 to oral tablets.
  • Pharmaceutical Preparative Example 26 is directed to use of 8-chloroadenosine 3',5'-cyclic phosphate sodium salt and/or 8-aminoadenosine 3',5'-cyclic phosphate sodium salt in suitable suppositories.
  • the ingredients are listed followed by the methods of preparing the composition.
  • the 8-chloroadenosine 3',5'-cyclic phosphate sodium salt and/or 8-aminoadenosine 3',5'-cyclic phosphate sodium salt is dissolved in the water and passed through a 0.22 ⁇ filter. The filtered solution is added to ampoules or vials, sealed and sterilized.

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Abstract

Les composés de phosphate 8-chloroadénosine 3',5'-cyclique et de phosphate 8-aminoadénosine 3',5'-cyclique sont utilisés ensemble ou séparément pour traiter des tumeurs malignes chez des animaux à sang chaud. Deux nouvelles synthèses à une seule étape du phosphate 8-chloroadénosine 3',5'-cyclique et d'autres composés d'adénine et d'adénosine apparentés obtenus à partir du phosphate d'adénosine 3',5'-cyclique correspondant et d'autres composés d'adénosine apparentés respectifs sont décrites.
EP19880905052 1987-12-21 1988-04-20 Treatment of malignant tumors with 8-chloroadenosine 3',5'-cyclic phosphate, 8-aminoadenosine 3',5'-cyclic phosphate and preparation thereof Withdrawn EP0346400A4 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US07/136,407 US4861873A (en) 1987-12-21 1987-12-21 8-Chloroadenosine 3', 5'-cyclic monophosphate preparations
US136407 1987-12-21
US18007088A 1988-04-11 1988-04-11
US180070 1988-04-11

Related Child Applications (1)

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EP19920106624 Division EP0499291A1 (fr) 1987-12-21 1988-04-20 Traitement de tumeurs malignes avec 8-chloroadenosine 3',5'-phosphate, cyclique 8-aminoadenosine 3',5'-phosphate cyclique et leur préparation

Publications (2)

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EP0346400A1 true EP0346400A1 (fr) 1989-12-20
EP0346400A4 EP0346400A4 (en) 1990-11-28

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EP19880905052 Withdrawn EP0346400A4 (en) 1987-12-21 1988-04-20 Treatment of malignant tumors with 8-chloroadenosine 3',5'-cyclic phosphate, 8-aminoadenosine 3',5'-cyclic phosphate and preparation thereof
EP19920106624 Withdrawn EP0499291A1 (fr) 1987-12-21 1988-04-20 Traitement de tumeurs malignes avec 8-chloroadenosine 3',5'-phosphate, cyclique 8-aminoadenosine 3',5'-phosphate cyclique et leur préparation

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Application Number Title Priority Date Filing Date
EP19920106624 Withdrawn EP0499291A1 (fr) 1987-12-21 1988-04-20 Traitement de tumeurs malignes avec 8-chloroadenosine 3',5'-phosphate, cyclique 8-aminoadenosine 3',5'-phosphate cyclique et leur préparation

Country Status (10)

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EP (2) EP0346400A4 (fr)
JP (1) JPH02502911A (fr)
KR (1) KR900700107A (fr)
AU (2) AU1933588A (fr)
CA (1) CA1326849C (fr)
DK (1) DK406589A (fr)
ES (1) ES2019713A6 (fr)
MX (1) MX168619B (fr)
NZ (1) NZ224307A (fr)
WO (2) WO1989005647A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04501254A (ja) * 1988-05-23 1992-03-05 アメリカ合衆国 癌治療剤としてのサイクリックａｍｐ誘導体
US4908441A (en) * 1988-07-19 1990-03-13 Warner-Lambert Company Deoxyadenosine compounds and methods of making and using the same
CA2085239A1 (fr) * 1990-06-15 1991-12-16 Junichi Yano Derives de nucleotide
CN1037575C (zh) * 1993-10-15 1998-03-04 北京市肿瘤防治研究所 8-氯腺苷制备抗肿瘤药物
GB201820685D0 (en) * 2018-12-19 2019-01-30 NuCana plc Synthesis of 8-chloroadenosine derivatives

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Publication number Priority date Publication date Assignee Title
GB1336441A (en) * 1969-10-10 1973-11-07 Anvar Derivatives of cyclo adenosine-3,5-phosphoric acid and their preparation
US3849553A (en) * 1973-09-04 1974-11-19 Nelson Res & Dev Cyclic nucleotide therapeutic preparation
US4369181A (en) * 1981-08-05 1983-01-18 Sri International Process for treating proliferative skin diseases using certain 6,8-substituted ribofuranosylpurine-3',5'-cyclic phosphates

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
See also references of WO8905648A1 *
THE JOURNAL OF ORGANIC CHEMISTRY, vol. 46, 19th June 1986; E.K. RYU: "New procedure for the chlorination of pyrimidine and purine nucleosides", pages 2819-2823 *

Also Published As

Publication number Publication date
CA1326849C (fr) 1994-02-08
WO1989005648A1 (fr) 1989-06-29
NZ224307A (en) 1990-03-27
EP0346400A4 (en) 1990-11-28
AU1933588A (en) 1989-07-19
EP0499291A1 (fr) 1992-08-19
AU1932888A (en) 1989-07-19
DK406589A (da) 1989-10-12
ES2019713A6 (es) 1991-07-01
KR900700107A (ko) 1990-08-11
JPH02502911A (ja) 1990-09-13
MX168619B (es) 1993-06-01
WO1989005647A1 (fr) 1989-06-29
DK406589D0 (da) 1989-08-18

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