EP0469919A3 - Verfahren zur enzymatischen Herstellung von 7-Aminocephalosporinsäure - Google Patents

Verfahren zur enzymatischen Herstellung von 7-Aminocephalosporinsäure Download PDF

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Publication number
EP0469919A3
EP0469919A3 EP19910307108 EP91307108A EP0469919A3 EP 0469919 A3 EP0469919 A3 EP 0469919A3 EP 19910307108 EP19910307108 EP 19910307108 EP 91307108 A EP91307108 A EP 91307108A EP 0469919 A3 EP0469919 A3 EP 0469919A3
Authority
EP
European Patent Office
Prior art keywords
coli
beta
acylase
carboxybutanamido
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP19910307108
Other languages
English (en)
French (fr)
Other versions
EP0469919A2 (de
EP0469919B1 (de
Inventor
Christian Croux
Javier Costa Perez
Jose Luis Barredo Fuentes
Francisco Salto Maldonado
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olon SpA
Original Assignee
Antibioticos SA
Antibioticos SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos SA, Antibioticos SpA filed Critical Antibioticos SA
Publication of EP0469919A2 publication Critical patent/EP0469919A2/de
Publication of EP0469919A3 publication Critical patent/EP0469919A3/de
Application granted granted Critical
Publication of EP0469919B1 publication Critical patent/EP0469919B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
EP91307108A 1990-08-03 1991-08-02 Verfahren zur enzymatischen Herstellung von 7-Aminocephalosporinsäure Expired - Lifetime EP0469919B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES9002109 1990-08-03
ES9002109A ES2020792A6 (es) 1990-08-03 1990-08-03 Un procedimiento enzimatico para la presentacion de acido 7-amino-cefa-losporanico y un procedimiento para expresar la enzima 7b (4-carboxibutanamido) cefalosporinacilasa.

Publications (3)

Publication Number Publication Date
EP0469919A2 EP0469919A2 (de) 1992-02-05
EP0469919A3 true EP0469919A3 (de) 1992-03-04
EP0469919B1 EP0469919B1 (de) 1997-04-09

Family

ID=8268451

Family Applications (1)

Application Number Title Priority Date Filing Date
EP91307108A Expired - Lifetime EP0469919B1 (de) 1990-08-03 1991-08-02 Verfahren zur enzymatischen Herstellung von 7-Aminocephalosporinsäure

Country Status (8)

Country Link
US (1) US5354667A (de)
EP (1) EP0469919B1 (de)
JP (1) JP3404406B2 (de)
AT (1) ATE151461T1 (de)
DE (1) DE69125541T2 (de)
DK (1) DK0469919T3 (de)
ES (1) ES2020792A6 (de)
GR (1) GR3023866T3 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0171897B1 (ko) * 1989-12-27 1999-02-01 후지사와 도모끼찌로 7-아미노세펨 화합물 또는 그 염류의 제조 방법
IT1269180B (it) * 1994-01-14 1997-03-21 Mini Ricerca Scient Tecnolog Processo per la produzione dell'enzima acido 7beta- (4-carbossibutanamido)-cefalosporanico acilasi
DE4437420A1 (de) * 1994-10-19 1996-04-25 Hoechst Ag Verbessertes, induktionsfreies Verfahren zur gentechnischen Herstellung von Glutarylamidase
ES2108652B1 (es) * 1996-04-19 1998-07-16 Antibioticos Sa Un procedimiento para modificar la enzima 7/3-(4-carboxibutanamido) cefalosporinacilasa y purificarla en un solo paso cromatografico.
FR2780416B1 (fr) * 1998-06-10 2002-12-20 Rhone Poulenc Nutrition Animal Procede industriel de production de proteines heterologues chez e. coli et souches utiles pour le procede
IT1312567B1 (it) * 1999-05-21 2002-04-22 Antibioticos Spa Processo per la sintesi di derivati beta-lattamici.
ES2252302T3 (es) 2000-09-22 2006-05-16 Bristol-Myers Squibb Company Amidasa de glutaril cefalosporina a partir de pseudomonas diminuta bs-203.
KR100530299B1 (ko) 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
CN1912127B (zh) * 2005-08-08 2011-05-25 百瑞全球有限公司 一种大肠杆菌表达载体及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0322032A2 (de) * 1987-12-21 1989-06-28 Merck & Co. Inc. Enzymatische Umwandlung von Cephalosporin C und Derivaten in 7-Aminocephalosporinsäure und Derivate in einem Schritt

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6121097A (ja) * 1984-07-10 1986-01-29 Asahi Chem Ind Co Ltd ７−アミノセフアロスポラン酸及びその誘導体の製造法
JPH04504362A (ja) * 1989-04-04 1992-08-06 バイオピユア・コーポレーシヨン 酵素による７―アミノセフアロスポラン酸の製造

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0322032A2 (de) * 1987-12-21 1989-06-28 Merck & Co. Inc. Enzymatische Umwandlung von Cephalosporin C und Derivaten in 7-Aminocephalosporinsäure und Derivate in einem Schritt

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
AGRICULTURAL AND BIOLOGICAL CHEMISTRY vol. 45, no. 7, July 1981, TOKYO pages 1561 - 1567; Yuzo Shibuya et al.: "Isolation and properties of 7 beta-[4-carboxybutanamido]cephalosporanic acid acyclase-producing bacteria" *
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY vol. 34, no. 6, June 1990, WASHINGTON US pages 943 - 948; S.W.QUEENER: "Molecular biology of penicillin and cephalosporin biosynthesis" *
CHEMICAL ABSTRACTS, vol. 110, no. 13, 1989 Columbus, Ohio, USA & JP-A-63 074 488 [Asahi Chem.Ind.Co.]4.4.88 page 557; ref. no. 113193Y *
JOURNAL OF BACTERIOLOGY vol. 169, no. 12, 1987, BALTIMORE US pages 5815 - 5820; Matsuda A. et al.: "Cloning and characterization of the genes for two distinct cephalosporin acylases from a Pseudomonas strain" *
JOURNAL OF BIOTECHNOLOGY vol. 3, no. 4, 1986, pages 187 - 195; Garcia J.L. et al.: "An improved method to clone penicillin acylase genes:cloning and expression in E.coli of penicillin G acylase from Kluyvera citrophila" *

Also Published As

Publication number Publication date
ES2020792A6 (es) 1991-09-16
DE69125541T2 (de) 1997-11-13
EP0469919A2 (de) 1992-02-05
EP0469919B1 (de) 1997-04-09
US5354667A (en) 1994-10-11
DK0469919T3 (da) 1997-07-21
GR3023866T3 (en) 1997-09-30
DE69125541D1 (de) 1997-05-15
JPH0690746A (ja) 1994-04-05
JP3404406B2 (ja) 2003-05-06
ATE151461T1 (de) 1997-04-15

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