EP2410314A3 - Procédé et appareil de tri de cellules - Google Patents

Procédé et appareil de tri de cellules Download PDF

Info

Publication number
EP2410314A3
EP2410314A3 EP11185768A EP11185768A EP2410314A3 EP 2410314 A3 EP2410314 A3 EP 2410314A3 EP 11185768 A EP11185768 A EP 11185768A EP 11185768 A EP11185768 A EP 11185768A EP 2410314 A3 EP2410314 A3 EP 2410314A3
Authority
EP
European Patent Office
Prior art keywords
stream
cells
detector
walls
forming
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP11185768A
Other languages
German (de)
English (en)
Other versions
EP2410314A2 (fr
Inventor
Daniel Mueth
Christopher R. Knutson
Amy Anderson
Joseph Plewa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Premium Genetics UK Ltd
Original Assignee
Arryx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36756165&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP2410314(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Arryx Inc filed Critical Arryx Inc
Priority to EP14172013.6A priority Critical patent/EP2801813A1/fr
Publication of EP2410314A2 publication Critical patent/EP2410314A2/fr
Publication of EP2410314A3 publication Critical patent/EP2410314A3/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N15/1409—Handling samples, e.g. injecting samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N15/1409—Handling samples, e.g. injecting samples
    • G01N2015/1411—Features of sheath fluids

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Electromagnetism (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP11185768A 2005-02-01 2006-02-01 Procédé et appareil de tri de cellules Withdrawn EP2410314A3 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP14172013.6A EP2801813A1 (fr) 2005-02-01 2006-02-01 Procédé et appareil de tri de cellules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/046,896 US7355696B2 (en) 2005-02-01 2005-02-01 Method and apparatus for sorting cells
EP06720022.0A EP1846748B1 (fr) 2005-02-01 2006-02-01 Procede et appareil pour trier des cellules

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP06720022.0A Division-Into EP1846748B1 (fr) 2005-02-01 2006-02-01 Procede et appareil pour trier des cellules
EP06720022.0 Division 2006-02-01

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP14172013.6A Division EP2801813A1 (fr) 2005-02-01 2006-02-01 Procédé et appareil de tri de cellules

Publications (2)

Publication Number Publication Date
EP2410314A2 EP2410314A2 (fr) 2012-01-25
EP2410314A3 true EP2410314A3 (fr) 2012-07-11

Family

ID=36756165

Family Applications (5)

Application Number Title Priority Date Filing Date
EP14172013.6A Withdrawn EP2801813A1 (fr) 2005-02-01 2006-02-01 Procédé et appareil de tri de cellules
EP11185768A Withdrawn EP2410314A3 (fr) 2005-02-01 2006-02-01 Procédé et appareil de tri de cellules
EP09176836.6A Expired - Lifetime EP2166340B1 (fr) 2005-02-01 2006-02-01 Procédés et appareil pour le tri de cellules
EP06720022.0A Expired - Lifetime EP1846748B1 (fr) 2005-02-01 2006-02-01 Procede et appareil pour trier des cellules
EP16182851.2A Withdrawn EP3109616A1 (fr) 2005-02-01 2006-02-01 Procede et appareil de tri de cellules

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP14172013.6A Withdrawn EP2801813A1 (fr) 2005-02-01 2006-02-01 Procédé et appareil de tri de cellules

Family Applications After (3)

Application Number Title Priority Date Filing Date
EP09176836.6A Expired - Lifetime EP2166340B1 (fr) 2005-02-01 2006-02-01 Procédés et appareil pour le tri de cellules
EP06720022.0A Expired - Lifetime EP1846748B1 (fr) 2005-02-01 2006-02-01 Procede et appareil pour trier des cellules
EP16182851.2A Withdrawn EP3109616A1 (fr) 2005-02-01 2006-02-01 Procede et appareil de tri de cellules

Country Status (9)

Country Link
US (3) US7355696B2 (fr)
EP (5) EP2801813A1 (fr)
JP (2) JP5086101B2 (fr)
CN (3) CN102128778B (fr)
BR (2) BRPI0607095B1 (fr)
DK (1) DK2166340T3 (fr)
ES (2) ES2639306T3 (fr)
HK (1) HK1203626A1 (fr)
WO (1) WO2006083907A2 (fr)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6808075B2 (en) * 2002-04-17 2004-10-26 Cytonome, Inc. Method and apparatus for sorting particles
US6976590B2 (en) 2002-06-24 2005-12-20 Cytonome, Inc. Method and apparatus for sorting particles
US9943847B2 (en) 2002-04-17 2018-04-17 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US20070065808A1 (en) * 2002-04-17 2007-03-22 Cytonome, Inc. Method and apparatus for sorting particles
US11243494B2 (en) 2002-07-31 2022-02-08 Abs Global, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
US7699767B2 (en) * 2002-07-31 2010-04-20 Arryx, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
US7311476B2 (en) * 2003-10-30 2007-12-25 Cytonome, Inc. Multilayer hydrodynamic sheath flow structure
US9260693B2 (en) 2004-12-03 2016-02-16 Cytonome/St, Llc Actuation of parallel microfluidic arrays
DE202005008763U1 (de) * 2005-06-02 2006-10-19 Universität Duisburg-Essen Vorrichtung zum Sortieren von Mikropartikeln, insbesondere biogefährdender und zu schützender Stoffe
GB0618605D0 (en) * 2006-09-21 2006-11-01 Univ St Andrews Optical sorting
JP4838094B2 (ja) * 2006-10-27 2011-12-14 三井造船株式会社 細胞分別処理機能を有するフローサイトメータ、および生細胞分別処理方法
US7701576B2 (en) * 2007-06-15 2010-04-20 Microstructure Technologies Inc. Method for sorting and analyzing particles in an aerosol with redundant particle analysis
US8663560B2 (en) * 2008-05-29 2014-03-04 Nippon Telegraph And Telephone Corporation Flow cell and liquid delivery method
UA102854C2 (ru) * 2008-06-30 2013-08-27 Микробикс Байосистемз Инк. Способ и устройство для сортирования клеток
US9314788B2 (en) * 2008-07-09 2016-04-19 Furukawa Electric Co., Ltd. Specimen identification and dispensation device and specimen identification and dispensation method
US9134221B2 (en) * 2009-03-10 2015-09-15 The Regents Of The University Of California Fluidic flow cytometry devices and particle sensing based on signal-encoding
WO2010132053A1 (fr) 2009-05-13 2010-11-18 Indevr, Inc. Mesure et commande de flux pour une quantification améliorée de particules dans une cytométrie de flux
US8665439B2 (en) * 2009-06-30 2014-03-04 Microbix Biosystems, Inc. Method and apparatus for limiting effects of refraction in cytometry
US9605298B2 (en) 2009-08-06 2017-03-28 Cornell University Device and methods for molecular analysis
JP5366727B2 (ja) * 2009-09-14 2013-12-11 北斗電子工業株式会社 液体中の粒子のサイズの検出方法および装置並びに光学装置
WO2011077765A1 (fr) * 2009-12-25 2011-06-30 古河電気工業株式会社 Dispositif et procédé d'identification et de séparation d'échantillons
SG183468A1 (en) * 2010-03-04 2012-09-27 Univ Singapore Microfluidics sorter for cell detection and isolation
US20110223586A1 (en) * 2010-03-11 2011-09-15 David Karabinus Optical particle characterization system
JP2011237201A (ja) * 2010-05-06 2011-11-24 Sony Corp 微小粒子分取装置、マイクロチップ及びマイクロチップモジュール
US20120225475A1 (en) * 2010-11-16 2012-09-06 1087 Systems, Inc. Cytometry system with quantum cascade laser source, acoustic detector, and micro-fluidic cell handling system configured for inspection of individual cells
US10908066B2 (en) 2010-11-16 2021-02-02 1087 Systems, Inc. Use of vibrational spectroscopy for microfluidic liquid measurement
MX338778B (es) 2011-02-15 2016-05-02 Microbix Biosystems Inc Metodos, sistemas y aparato para realizar citometrias de flujo.
WO2012161764A1 (fr) * 2011-02-16 2012-11-29 Fox Daniel N Tri magnétique de sperme de mammifère ayant des membranes lésées
JP2012189483A (ja) * 2011-03-11 2012-10-04 Fuji Electric Co Ltd 粒子測定装置
US9781919B2 (en) 2011-06-01 2017-10-10 Inguran, Llc Compositions and methods for improving the quality of processed sperm
WO2012167151A1 (fr) 2011-06-01 2012-12-06 Inguran, Llc Compositions et procédés pour améliorer la qualité du sperme traité
JP5910412B2 (ja) * 2012-08-16 2016-04-27 ソニー株式会社 微小粒子分取方法及び微小粒子分取用マイクロチップ
WO2014047206A1 (fr) * 2012-09-18 2014-03-27 Cytonome/St, Llc Cuve à circulation pour le tri de particules
CN110579435B (zh) 2012-10-15 2023-09-26 纳诺赛莱克特生物医药股份有限公司 颗粒分选的系统、设备和方法
EP2757402B1 (fr) * 2013-01-22 2016-03-30 FEI Company Procédé d'observation d'échantillons avec un microscope à fluorescence
JP2014174139A (ja) * 2013-03-13 2014-09-22 Sony Corp 流路デバイス、粒子分取装置、粒子流出方法、及び粒子分取方法
NZ743491A (en) 2013-03-14 2020-03-27 Cytonome St Llc Hydrodynamic focusing apparatus and methods
US10662408B2 (en) 2013-03-14 2020-05-26 Inguran, Llc Methods for high throughput sperm sorting
US10371622B2 (en) 2013-03-14 2019-08-06 Inguran, Llc Device for high throughput sperm sorting
WO2014142924A1 (fr) * 2013-03-14 2014-09-18 Inguran, Llc Appareil et procédés pour tri de sperme à haut rendement
US9757726B2 (en) * 2013-03-14 2017-09-12 Inguran, Llc System for high throughput sperm sorting
US8961904B2 (en) * 2013-07-16 2015-02-24 Premium Genetics (Uk) Ltd. Microfluidic chip
JP6205055B2 (ja) * 2013-07-16 2017-09-27 プレミアム ジェネティクス （ユーケー） リミテッド マイクロ流体チップ
JP6554100B2 (ja) 2013-08-16 2019-07-31 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド フローサイトメーターにおいて流体ストリームから液滴を分離及び／又は帯電させるタイミング及び／又は位相の調整
US11796449B2 (en) 2013-10-30 2023-10-24 Abs Global, Inc. Microfluidic system and method with focused energy apparatus
JP6733909B2 (ja) * 2013-10-30 2020-08-05 エービーエス グローバル インコーポレイテッド 複数の物質を識別する装置、複数の物質を識別するコンピュータシステム、複数の物質を識別するための複数の命令をコンピュータに実行させるプログラムおよびサンプル流体混合物中を流れる複数の物質を識別する方法
US9663755B2 (en) 2013-11-19 2017-05-30 The Governing Council Of The University Of Toronto Apparatus and methods for sperm separation
US9939362B2 (en) * 2014-02-20 2018-04-10 Malvern Instruments Limited Heterogeneous fluid sample characterization
CN106660004A (zh) * 2014-05-08 2017-05-10 公立大学法人大阪府立大学 聚集装置及聚集方法、微小物体聚集结构体的制造装置、微生物的聚集除去装置、被检测物质的检测装置、被分离物质的分离装置以及被导入物质的导入装置
PL2997823T3 (pl) * 2014-09-19 2018-06-29 Instruments Utils De Laboratori Geniul, Sl Procedura poprawy jakości spermy u ssaków
US10180388B2 (en) 2015-02-19 2019-01-15 1087 Systems, Inc. Scanning infrared measurement system
WO2016144562A1 (fr) * 2015-03-06 2016-09-15 Becton, Dickinson And Company Systèmes de collecte de lumière et leurs procédés de fabrication et d'utilisation
CN107532990B (zh) * 2015-05-12 2021-11-12 芯片生物技术株式会社 单一粒子解析方法及用于该解析的系统
CN105486667A (zh) * 2015-07-01 2016-04-13 上海睿钰生物科技有限公司 集成式荧光激发光源装置
WO2017053478A1 (fr) 2015-09-22 2017-03-30 Wyatt Technology Corporation Procédé et appareil permettant de mesurer de multiples signaux à partir d'un échantillon liquide
KR102578808B1 (ko) * 2016-01-21 2023-09-15 도쿄엘렉트론가부시키가이샤 이물 검출 장치 및 이물 검출 방법
CN108780031A (zh) * 2016-03-30 2018-11-09 西门子保健有限责任公司 使用环境粘弹性流体流对准样本流中的非球形生物实体
CN107442186B (zh) * 2016-05-31 2020-06-09 深圳汇芯生物医疗科技有限公司 微流控芯片、基于微流控芯片的分析装置及分析方法
WO2018175411A1 (fr) 2017-03-20 2018-09-27 Nanocellect Biomedical, Inc. Systèmes, appareils et procédés de tri de cellules et cytométrie de flux
USD868991S1 (en) 2017-03-28 2019-12-03 Becton, Dickinson And Company Register block
USD869676S1 (en) 2017-03-28 2019-12-10 Becton, Dickinson And Company Particle sorting module
EP3654018B1 (fr) * 2017-07-11 2023-06-21 Hamamatsu Photonics K.K. Dispositif d'observation d'échantillon et procédé d'observation d'échantillon
CN111630364A (zh) * 2018-01-29 2020-09-04 惠普发展公司，有限责任合伙企业 颗粒分类
USD864415S1 (en) 2018-01-30 2019-10-22 Becton, Dickinson And Company Particle sorting system
USD872296S1 (en) 2018-01-30 2020-01-07 Becton, Dickinson And Company Particle sorting module
USD876668S1 (en) 2018-01-30 2020-02-25 Becton, Dickinson And Company Particle sorting module mount
USD882817S1 (en) 2018-01-30 2020-04-28 Becton, Dickinson And Company Sample container
US11022538B2 (en) * 2018-05-15 2021-06-01 Government Of The United States Of America, As Represented By The Secretary Of Commerce Quantum flow cytometer
US11331670B2 (en) 2018-05-23 2022-05-17 Abs Global, Inc. Systems and methods for particle focusing in microchannels
CN109082363A (zh) * 2018-07-23 2018-12-25 上海市第人民医院 一种优选精子冷冻培养设备
WO2020028639A1 (fr) 2018-08-01 2020-02-06 Essen Instruments, Inc. D/B/A Essen Bioscience, Inc. Procédés, kits et compositions de coloration pour l'évaluation par cytométrie de flux de particules de taille virale non associées à l'aide de multiples colorants fluorogènes
CN111073817A (zh) * 2018-10-22 2020-04-28 曾繁根 用于精子分选、卵子培育、体外受精的整合式晶片及方法
EP3666881A1 (fr) 2018-12-12 2020-06-17 AIT Austrian Institute of Technology GmbH Tri couplés et traitement électrique de cellules biologiques
EP3899489B1 (fr) 2018-12-21 2025-04-09 ABS Global, Inc. Système et procédés pour identification de sous-populations
US11137337B2 (en) 2019-01-21 2021-10-05 Essen Instruments, Inc. Flow cytometry with data analysis for optimized dilution of fluid samples for flow cytometry investigation
WO2020175381A1 (fr) * 2019-02-27 2020-09-03 京セラ株式会社 Dispositif de mesure et de séparation de particules, et appareil de mesure et de séparation de particules
EP3948279B1 (fr) 2019-03-26 2025-06-18 Sartorius BioAnalytical Instruments, Inc. Évaluation par cytométrie en flux pour particules virales non enrobées non associées
EP3955735B1 (fr) 2019-04-18 2024-05-22 ABS Global, Inc. Système et procédé d'ajout en continu de cryoprotecteur
US11628439B2 (en) 2020-01-13 2023-04-18 Abs Global, Inc. Single-sheath microfluidic chip
US11709116B2 (en) 2020-02-04 2023-07-25 Sartorius Bioanalytical Instruments, Inc. Liquid flourescent dye concentrate for flow cytometry evaluation of virus-size particles and related products and methods
US12357991B2 (en) 2020-05-15 2025-07-15 David Sinton Sperm selection device, kit, and methods
WO2022087299A1 (fr) 2020-10-21 2022-04-28 Abs Global, Inc. Procédés et systèmes de traitement d'échantillons génétiques pour déterminer l'identité ou détecter une contamination
US12135270B2 (en) 2020-11-23 2024-11-05 Abs Global, Inc. Modular flow cytometry systems and methods of processing samples
EP4308903A4 (fr) * 2021-03-17 2025-01-22 ABS Global, Inc. Différenciation cellulaire basée sur une lumière multidirectionnelle provenant d'une puce microfluidique
USD1118965S1 (en) 2021-11-12 2026-03-17 Abs Global, Inc. Flow cytometry device

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4983038A (en) * 1987-04-08 1991-01-08 Hitachi, Ltd. Sheath flow type flow-cell device
US5180065A (en) * 1989-10-11 1993-01-19 Canon Kabushiki Kaisha Apparatus for and method of fractionating particle in particle-suspended liquid in conformity with the properties thereof
US5311290A (en) * 1992-09-30 1994-05-10 Pulp And Paper Research Institute Of Canada Imaging apparatus and method of fiber analysis
US5483469A (en) * 1993-08-02 1996-01-09 The Regents Of The University Of California Multiple sort flow cytometer
US6149867A (en) * 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US20020127736A1 (en) * 2000-10-03 2002-09-12 California Institute Of Technology Microfluidic devices and methods of use
US6473172B1 (en) * 2000-09-20 2002-10-29 International Remote Imaging Systems, Inc. Flow cell and method of operating therefor
US20030054558A1 (en) * 2001-07-18 2003-03-20 Katsuo Kurabayashi Flow cytometers and detection system of lesser size
US20030196714A1 (en) * 2002-04-17 2003-10-23 Coventor, Inc. Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
WO2005008221A1 (fr) * 2003-07-08 2005-01-27 Applera Corporation Integration dans le temps dans un systeme de cytometrie de flux

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3649829A (en) * 1970-10-06 1972-03-14 Atomic Energy Commission Laminar flow cell
US3830568A (en) * 1973-05-25 1974-08-20 Texas Instruments Inc Multiple detection volume laser doppler velocimeter
US3960449A (en) * 1975-06-05 1976-06-01 The Board Of Trustees Of Leland Stanford Junior University Measurement of angular dependence of scattered light in a flowing stream
US4667830A (en) * 1981-06-15 1987-05-26 The Board Of Trustees Of The Leland Stanford Junior University Method and means for sorting individual particles into containers for culturing, cloning, analysis, or the like
US4395397A (en) * 1981-09-17 1983-07-26 Sidney Farber Cancer Institute, Inc. Apparatus and method for killing unwanted cells
US4660971A (en) * 1984-05-03 1987-04-28 Becton, Dickinson And Company Optical features of flow cytometry apparatus
GB8523747D0 (en) * 1985-09-26 1985-10-30 Vg Instr Group Fibre size monitor
JPS62168033A (ja) * 1986-01-18 1987-07-24 Canon Inc 粒子解析装置
US4873653A (en) * 1986-04-09 1989-10-10 Carl-Zeiss-Stiftung Microscope system for providing three-dimensional resolution
JPH07119686B2 (ja) * 1987-04-08 1995-12-20 株式会社日立製作所 フローセル装置
JPS63262565A (ja) * 1987-04-20 1988-10-28 Hitachi Ltd フロ−セル
JPS6474451A (en) * 1987-09-16 1989-03-20 Showa Denko Kk Sell selection device
JPH02176562A (ja) * 1988-12-28 1990-07-09 Hitachi Ltd 分析装置
CA2055494C (fr) 1989-05-10 2000-01-18 Lawrence Arthur Johnson Methode de preselection du sexe d'un enfant
JPH03125944A (ja) * 1989-10-11 1991-05-29 Canon Inc 粒子分別装置
FR2653885B1 (fr) * 1989-10-27 1994-01-14 Abx Appareil pour le comptage et la determination d'au moins une sous-population leucocytaire.
US5194909A (en) * 1990-12-04 1993-03-16 Tycko Daniel H Apparatus and method for measuring volume and hemoglobin concentration of red blood cells
JP3075367B2 (ja) 1991-04-05 2000-08-14 シスメックス株式会社 粒子分析方法及び装置
JPH0510946A (ja) * 1991-07-03 1993-01-19 Fuji Electric Co Ltd 細胞解析装置
US5548395A (en) * 1991-09-20 1996-08-20 Toa Medical Electronics Co., Ltd. Particle analyzer
JP3212647B2 (ja) * 1991-10-24 2001-09-25 シスメックス株式会社 イメージングフローサイトメータ
US5635402A (en) * 1992-03-05 1997-06-03 Alfano; Robert R. Technique for determining whether a cell is malignant as opposed to non-malignant using extrinsic fluorescence spectroscopy
JPH07286953A (ja) * 1994-04-19 1995-10-31 Toa Medical Electronics Co Ltd イメージングフローサイトメータ
US5707808A (en) * 1996-04-15 1998-01-13 The Regents Of The University Of California Optical selection and collection of DNA fragments
US5936739A (en) * 1997-01-29 1999-08-10 Sandia Corporation Gated frequency-resolved optical imaging with an optical parametric amplifier
EP1396736A3 (fr) * 1997-03-11 2004-12-29 Nihon Kohden Corporation Analyseur de particules et lentille-composite formée de lentilles de foyers differents
JP3679601B2 (ja) * 1997-03-11 2005-08-03 日本分光株式会社 粒子分析装置及び複数の焦点位置を有するレンズ要素を一体化した複合レンズ
US5985216A (en) * 1997-07-24 1999-11-16 The United States Of America, As Represented By The Secretary Of Agriculture Flow cytometry nozzle for high efficiency cell sorting
US5956139A (en) * 1997-08-04 1999-09-21 Ohio Aerospace Institute Cross-correlation method and apparatus for suppressing the effects of multiple scattering
US6071689A (en) * 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6388788B1 (en) * 1998-03-16 2002-05-14 Praelux, Inc. Method and apparatus for screening chemical compounds
US7116407B2 (en) * 1998-12-15 2006-10-03 Union Biometrica, Inc. System for axial pattern analysis of multicellular organisms
US6256096B1 (en) * 1999-01-11 2001-07-03 Softray Flow cytometry apparatus and method
US6975400B2 (en) * 1999-01-25 2005-12-13 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US6249341B1 (en) * 1999-01-25 2001-06-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US6628385B1 (en) * 1999-02-05 2003-09-30 Axon Instruments, Inc. High efficiency, large field scanning microscope
EP1179087B1 (fr) 1999-05-17 2019-03-27 Caliper Life Sciences, Inc. Focalisation de microparticules dans des systemes microfluidiques
US7126687B2 (en) * 1999-08-09 2006-10-24 The United States Of America As Represented By The Secretary Of The Army Method and instrumentation for determining absorption and morphology of individual airborne particles
US6813017B1 (en) 1999-10-20 2004-11-02 Becton, Dickinson And Company Apparatus and method employing incoherent light emitting semiconductor devices as particle detection light sources in a flow cytometer
FR2801671B1 (fr) * 1999-11-29 2001-12-21 Commissariat Energie Atomique Dispositif de mesure, par diffraction, de tailles de particules sensiblement spheriques, notamment de gouttes opaques
US6263745B1 (en) 1999-12-03 2001-07-24 Xy, Inc. Flow cytometer nozzle and flow cytometer sample handling methods
AU2001279026B2 (en) * 2000-07-25 2005-12-22 Angiodynamics, Inc. Apparatus for detecting and treating tumors using localized impedance measurement
WO2002019594A2 (fr) * 2000-08-02 2002-03-07 Arizona Board Of Regents, Acting On Behalf Of Arizona State University Microscope de determination de la duree de vie de fluorescence a balayage a interface informatique utilise dans les techniques d'evolution dirigee et methodes de structuration photo-induite utilisees dans la selection cellulaire
US6875973B2 (en) * 2000-08-25 2005-04-05 Amnis Corporation Auto focus for a flow imaging system
US6507391B2 (en) * 2000-08-25 2003-01-14 Amnis Corporation Measuring the velocity of small moving objects such as cells
US20020028434A1 (en) * 2000-09-06 2002-03-07 Guava Technologies, Inc. Particle or cell analyzer and method
CA2454340A1 (fr) * 2000-11-22 2002-05-30 Pharmacia Corporation Procedes et appareil de production de spermatozoides a enrichissement par sexe (ges)
AU2002308693A1 (en) * 2001-04-25 2002-11-05 Amnis Corporation Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging
JP2003106980A (ja) * 2001-10-01 2003-04-09 Nec Corp 微小粒子群の計測装置および計測方法
US6794671B2 (en) 2002-07-17 2004-09-21 Particle Sizing Systems, Inc. Sensors and methods for high-sensitivity optical particle counting and sizing
CN102539303B (zh) * 2002-07-31 2016-06-15 普瑞姆遗传(英国)有限公司 利用全息激光控制分类物质的系统和方法
US7118676B2 (en) * 2003-09-04 2006-10-10 Arryx, Inc. Multiple laminar flow-based particle and cellular separation with laser steering
JP3891925B2 (ja) * 2002-12-03 2007-03-14 ベイバイオサイエンス株式会社 生物学的粒子の情報を得る装置
BRPI0408857B1 (pt) * 2003-03-28 2018-09-11 Inguran Llc aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo
WO2004087177A1 (fr) * 2003-03-28 2004-10-14 Monsanto Technology Llc Procédé de coloration du sperme
WO2004092781A2 (fr) * 2003-04-11 2004-10-28 Amnis Corporation Dispositif de focalisation automatique pour systeme d'imagerie en flux continu
ES2541121T3 (es) * 2003-05-15 2015-07-16 Xy, Llc Clasificación eficiente de células haploides por sistemas de citometría de flujo
US7115230B2 (en) 2003-06-26 2006-10-03 Intel Corporation Hydrodynamic focusing devices
US7260485B2 (en) * 2003-07-18 2007-08-21 Luminex Corporation Method and systems for distinguishing between materials having similar spectra

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4983038A (en) * 1987-04-08 1991-01-08 Hitachi, Ltd. Sheath flow type flow-cell device
US5180065A (en) * 1989-10-11 1993-01-19 Canon Kabushiki Kaisha Apparatus for and method of fractionating particle in particle-suspended liquid in conformity with the properties thereof
US5311290A (en) * 1992-09-30 1994-05-10 Pulp And Paper Research Institute Of Canada Imaging apparatus and method of fiber analysis
US5483469A (en) * 1993-08-02 1996-01-09 The Regents Of The University Of California Multiple sort flow cytometer
US6149867A (en) * 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6473172B1 (en) * 2000-09-20 2002-10-29 International Remote Imaging Systems, Inc. Flow cell and method of operating therefor
US20020127736A1 (en) * 2000-10-03 2002-09-12 California Institute Of Technology Microfluidic devices and methods of use
US20030054558A1 (en) * 2001-07-18 2003-03-20 Katsuo Kurabayashi Flow cytometers and detection system of lesser size
US20030196714A1 (en) * 2002-04-17 2003-10-23 Coventor, Inc. Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
WO2005008221A1 (fr) * 2003-07-08 2005-01-27 Applera Corporation Integration dans le temps dans un systeme de cytometrie de flux

Also Published As

Publication number Publication date
CN102212457B (zh) 2014-09-10
BRPI0607095A2 (pt) 2009-08-04
EP1846748A2 (fr) 2007-10-24
ES2639306T3 (es) 2017-10-26
USRE46992E1 (en) 2018-08-14
CN101189504B (zh) 2012-05-30
JP2008530985A (ja) 2008-08-14
US20060170912A1 (en) 2006-08-03
CN102128778A (zh) 2011-07-20
CN101189504A (zh) 2008-05-28
CN102128778B (zh) 2013-12-18
BRPI0607095B1 (pt) 2017-11-21
WO2006083907A3 (fr) 2006-12-21
JP5086101B2 (ja) 2012-11-28
EP1846748A4 (fr) 2009-05-20
ES2595047T3 (es) 2016-12-27
DK2166340T3 (en) 2017-09-18
EP1846748B1 (fr) 2016-09-14
JP2012013714A (ja) 2012-01-19
EP2166340A1 (fr) 2010-03-24
US20080144037A1 (en) 2008-06-19
US7545491B2 (en) 2009-06-09
EP2166340B1 (fr) 2017-08-02
HK1160222A1 (en) 2012-08-10
EP3109616A1 (fr) 2016-12-28
HK1112288A1 (en) 2008-08-29
HK1203626A1 (en) 2015-10-30
WO2006083907A2 (fr) 2006-08-10
EP2801813A1 (fr) 2014-11-12
CN102212457A (zh) 2011-10-12
EP2410314A2 (fr) 2012-01-25
US7355696B2 (en) 2008-04-08
BR122017014567B1 (pt) 2018-04-17

Similar Documents

Publication Publication Date Title
EP2410314A3 (fr) Procédé et appareil de tri de cellules
ATE279263T1 (de) Vorrichtung zum abtrennen von teilchen aus einem fluidstrom
EP1557657A3 (fr) Dispositif et procédé de triage de particules
WO2005023391A3 (fr) Particule basee sur flux laminaire multiple et separation cellulaire a guidage laser
DE60321006D1 (de) Vorrichtung und verfahren zum trennen von teilchen
US11167293B2 (en) Cyclone separator
WO2006115938A3 (fr) Procede et appareil permettant de separer des particules en suspension dans un liquide
DE69524591D1 (de) Vorrichtung und methoden zum abtrennen von feststoffen aus strömenden flüssigkeiten oder gasen
WO2008121229A8 (fr) Procédé et appareil pour concentrer des molécules
WO2008148028A3 (fr) Procédés et appareils pour séparer des particules biologiques
Zheng et al. Streamline-based microfluidic devices for erythrocytes and leukocytes separation
RU2008148012A (ru) Способ и устройство для получения и/или кондиционирования порошкообразного материала
Roberts et al. Flow‐induced particulate separations
WO2004088277A3 (fr) Systeme et procede bases sur la gestion de l'energie gazeuse pour effectuer la flottation et la separation de particules
EP2103354A3 (fr) Procédé de classification et appareil de classification
CN103501911A (zh) 用于粒子分离的方法和装置
AU2016311458A1 (en) Apparatus and method for gravity separation related to oil and natural gas production systems and methods
US8252088B2 (en) Particle concentrator
WO2004056441A3 (fr) Procede et appareil pour une separation de melange
WO2006102258A2 (fr) Tri de particules par deflexion fluidique
JP2006263693A (ja) 微粒子の連続分離機構及び装置
DE602004022563D1 (de) Vorrichtung zur beförderung von gasen in eine kammer und aus einer kammer
WO2016196204A1 (fr) Appareil et procédé de séparation par gravité et système de production de pétrole et de gaz
DE502006007156D1 (de) Vorrichtung zum sichten von aufgabegut
WO2006109036A3 (fr) Appareil et procede de dilution

Legal Events

Date Code Title Description
17P Request for examination filed

Effective date: 20111019

AC Divisional application: reference to earlier application

Ref document number: 1846748

Country of ref document: EP

Kind code of ref document: P

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

RIC1 Information provided on ipc code assigned before grant

Ipc: G01N 21/01 20060101AFI20120120BHEP

Ipc: G01N 33/00 20060101ALI20120120BHEP

Ipc: G01N 15/14 20060101ALI20120120BHEP

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013

AK Designated contracting states

Kind code of ref document: A3

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

RIC1 Information provided on ipc code assigned before grant

Ipc: G01N 21/01 20060101AFI20120604BHEP

Ipc: G01N 33/00 20060101ALI20120604BHEP

Ipc: G01N 15/14 20060101ALI20120604BHEP

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: PREMIUM GENETICS (UK) LIMITED

17Q First examination report despatched

Effective date: 20161109

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20190305

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20190716