EP3359693A4 - Verfahren und zusammensetzungen zur anreicherung von amplifikationsprodukten - Google Patents

Verfahren und zusammensetzungen zur anreicherung von amplifikationsprodukten Download PDF

Info

Publication number
EP3359693A4
EP3359693A4 EP16854489.8A EP16854489A EP3359693A4 EP 3359693 A4 EP3359693 A4 EP 3359693A4 EP 16854489 A EP16854489 A EP 16854489A EP 3359693 A4 EP3359693 A4 EP 3359693A4
Authority
EP
European Patent Office
Prior art keywords
enrichment
compositions
methods
amplification products
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP16854489.8A
Other languages
English (en)
French (fr)
Other versions
EP3359693A1 (de
EP3359693B1 (de
Inventor
Li Weng
Shengrong LIN
Ling Fung TANG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Accuragen Holdings Ltd
Original Assignee
Accuragen Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Accuragen Holdings Ltd filed Critical Accuragen Holdings Ltd
Publication of EP3359693A1 publication Critical patent/EP3359693A1/de
Publication of EP3359693A4 publication Critical patent/EP3359693A4/de
Application granted granted Critical
Publication of EP3359693B1 publication Critical patent/EP3359693B1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/307—Circular oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/16—Assays for determining copy number or wherein the copy number is of special importance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Macromonomer-Based Addition Polymer (AREA)
  • Enzymes And Modification Thereof (AREA)
EP16854489.8A 2015-10-09 2016-10-07 Verfahren zur anreicherung von amplifikationsprodukten Active EP3359693B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562239690P 2015-10-09 2015-10-09
PCT/US2016/056126 WO2017062863A1 (en) 2015-10-09 2016-10-07 Methods and compositions for enrichment of amplification products

Publications (3)

Publication Number Publication Date
EP3359693A1 EP3359693A1 (de) 2018-08-15
EP3359693A4 true EP3359693A4 (de) 2019-03-06
EP3359693B1 EP3359693B1 (de) 2025-03-19

Family

ID=58488563

Family Applications (1)

Application Number Title Priority Date Filing Date
EP16854489.8A Active EP3359693B1 (de) 2015-10-09 2016-10-07 Verfahren zur anreicherung von amplifikationsprodukten

Country Status (10)

Country Link
US (3) US10752942B2 (de)
EP (1) EP3359693B1 (de)
JP (3) JP7008016B2 (de)
CN (2) CN108368545B (de)
AU (2) AU2016334233B2 (de)
HK (1) HK1259101A1 (de)
IL (2) IL258236B (de)
MX (2) MX2018004284A (de)
SG (1) SG11201802864RA (de)
WO (1) WO2017062863A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
KR20240025725A (ko) 2013-12-11 2024-02-27 아큐라젠 홀딩스 리미티드 희귀 서열 변이를 검출하기 위한 조성물 및 방법
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
MX2018004284A (es) 2015-10-09 2018-05-16 Accuragen Holdings Ltd Metodos y composiciones para el enriquecimiento de productos de amplificacion.
EP3383994A4 (de) * 2015-12-03 2019-08-28 Accuragen Holdings Limited Verfahren und zusammensetzungen zum formen von ligationsprodukten
CN109511265B (zh) 2016-05-16 2023-07-14 安可济控股有限公司 通过链鉴定改进测序的方法
EP4257701A3 (de) 2016-06-30 2023-12-20 Grail, LLC Differenzielle markierung von rna zur herstellung einer zellfreien dna/rna-sequenzierungsbibliothek
WO2018035170A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
CN107760763B (zh) * 2017-11-20 2021-05-11 东南大学 一种用于茎环引物-滚环扩增反应的茎环引物以及茎环引物-滚环扩增的应用
CN109971825B (zh) * 2017-12-28 2020-11-10 南京金斯瑞生物科技有限公司 快速制备桑格测序模板的方法
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
WO2019241290A1 (en) 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products
EP3650558A1 (de) * 2018-11-07 2020-05-13 Siemens Healthcare GmbH Arbeitsablauf zur nanoporensequenzierung von flüssigproben
CN110820051B (zh) * 2018-12-28 2023-04-28 广州表观生物科技有限公司 一种高灵敏度融合基因检测方法及其应用
WO2020263978A1 (en) * 2019-06-25 2020-12-30 Accuragen Holdings Limited Methods and systems for disease detection
GB201911421D0 (en) * 2019-08-09 2019-09-25 Dnae Diagnostics Ltd Methods of multiplexed isothermal amplification of nucleic acid sequences
WO2021046655A1 (en) * 2019-09-13 2021-03-18 University Health Network Detection of circulating tumor dna using double stranded hybrid capture
WO2021101835A1 (en) * 2019-11-19 2021-05-27 Xiao Bing Wang Method for identifying gene fusions by circle cdna amplification
CN115151657A (zh) * 2019-12-20 2022-10-04 安可济控股有限公司 用于疾病检测的方法和系统
KR102468839B1 (ko) * 2020-06-03 2022-11-18 주식회사 제노헬릭스 Rna 검출 방법
AU2021294360A1 (en) * 2020-06-26 2023-02-02 William Marsh Rice University Amplicon Comprehensive Enrichment
AU2021366658A1 (en) * 2020-10-21 2023-06-22 Illumina Cambridge Limited Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
WO2022103750A1 (en) * 2020-11-11 2022-05-19 Accuragen Holdings Limited Methods and systems for sample normalization
CN113549674B (zh) * 2021-04-22 2023-11-03 福建和瑞基因科技有限公司 一种检测样本中目标序列整合和突变的方法及其引物的设计方法和试剂盒
CN113832147B (zh) * 2021-09-08 2024-06-14 华南农业大学 一种高效的大片段dna合成与扩增的pcr引物、方法及应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems

Family Cites Families (155)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US443087A (en) * 1890-12-23 Riding-saddle
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US6677312B1 (en) 1989-03-29 2004-01-13 The Johns Hopkins University Methods for restoring wild-type p53 gene function
EP0390323B2 (de) 1989-03-29 2012-08-08 Johns Hopkins University Nachweis des Ausfalls des Wildtyps des p53-Gens
US5527676A (en) 1989-03-29 1996-06-18 The Johns Hopkins University Detection of loss of the wild-type P53 gene and kits therefor
US6800617B1 (en) 1989-03-29 2004-10-05 The Johns Hopkins University Methods for restoring wild-type p53 gene function
US5362623A (en) 1991-06-14 1994-11-08 The John Hopkins University Sequence specific DNA binding by p53
US5480784A (en) 1989-07-11 1996-01-02 Gen-Probe Incorporated Nucleic acid sequence amplification methods
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5527670A (en) 1990-09-12 1996-06-18 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
AU1366992A (en) 1991-01-16 1992-08-27 Cancer Institute, The Inherited and somatic mutations of apc gene in colorectal cancer of humans
US5352775A (en) 1991-01-16 1994-10-04 The Johns Hopkins Univ. APC gene and nucleic acid probes derived therefrom
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5330892A (en) 1991-03-13 1994-07-19 The Johns Hopkins University MCC gene (mutated in colorectal cancer) used for diagnosis of cancer in humans
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5591826A (en) 1993-05-05 1997-01-07 The Johns Hopkins University Human MSH2 protein
US5492808A (en) 1993-05-05 1996-02-20 The Johns Hopkins University Means for detecting familial colon cancer (FCC)
US7326778B1 (en) 1993-05-05 2008-02-05 The John Hopkins University Mutator gene and hereditary non-polyposis colorectal cancer
ES2114692T3 (es) 1993-06-09 1998-06-01 Gamera Bioscience Corp Reaccion en ciclos magneticos.
WO1995013375A1 (en) 1993-11-10 1995-05-18 The Johns Hopkins University Tumor suppressor waf1
ATE273387T1 (de) 1993-12-02 2004-08-15 Univ Johns Hopkins Menschliches mutator-gen nmsh2 und seine verbindung zum hereditaren, nichtpolypösen kolorrektalen karzinom
US6380369B1 (en) 1994-01-27 2002-04-30 Human Genome Sciences, Inc. Human DNA mismatch repair proteins
NZ281023A (en) 1994-01-27 1998-04-27 Human Genome Sciences Inc Human dna mismatch repair proteins and coding sequences
US6482606B1 (en) 1994-01-27 2002-11-19 Human Genome Sciences, Inc. Human DNA mismatch repair polynucleotides
ATE175723T1 (de) 1994-03-15 1999-01-15 Scient Generics Ltd Elektrochemische denatusierung von doppelsträngiger nukleinsäure
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US6593086B2 (en) * 1996-05-20 2003-07-15 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5648245A (en) * 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US6143495A (en) * 1995-11-21 2000-11-07 Yale University Unimolecular segment amplification and sequencing
US6511805B1 (en) 1996-03-15 2003-01-28 The Penn State Research Foundation Methods for detecting papillomavirus DNA in blood plasma and serum
US8048629B2 (en) 1996-03-15 2011-11-01 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
US6156504A (en) 1996-03-15 2000-12-05 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
ES2347854T5 (es) 1996-03-26 2014-07-24 Michael S. Kopreski Método que permite el uso de RNA extracelular extraído de plasma o suero para detectar, monitorizar o evaluar un cáncer
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
EP1591541B1 (de) 1997-04-01 2012-02-15 Illumina Cambridge Limited Verfahren zur Vervielfältigung von Nukleinsäuren
GB9706654D0 (en) 1997-04-02 1997-05-21 Scient Generics Ltd Disassociation of interacting molecules
WO1998045474A1 (en) 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
WO1999053102A1 (en) * 1998-04-16 1999-10-21 Packard Bioscience Company Analysis of polynucleotide sequence
US6410278B1 (en) 1998-11-09 2002-06-25 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
GB9903906D0 (en) 1999-02-19 1999-04-14 Microbiological Res Authority Method and apparatus for nucleic acid strand separation
DE60033647T2 (de) 1999-03-17 2007-11-15 The Board Of Trustees Of The Leland Stanford Junior University, Stanford Detektion von fehlbasenpaarungen
CA2364572A1 (en) * 1999-04-12 2000-10-19 Genentech, Inc. Pcr-based cloning method
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
AU7364700A (en) 1999-09-08 2001-04-10 University Of Victoria Innovation And Development Corporation Use of psychrotrophic bacterium in biotechnology applications
CA2384838C (en) 1999-09-13 2006-07-18 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
EP1234058A2 (de) 1999-11-26 2002-08-28 Curagen Corporation Nukleinsäuresondenarrays
US6653079B2 (en) 2000-03-13 2003-11-25 Freshgene, Inc. Methods for detection of differences in nucleic acids
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US6626906B1 (en) 2000-10-23 2003-09-30 Sdgi Holdings, Inc. Multi-planar adjustable connector
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
DE60231606D1 (de) * 2001-02-27 2009-04-30 Virco Bvba Zirkuläre sonden amplifizierung (cpa) von zirkularisierten nucleinsäure-molekülen
KR20030082535A (ko) 2001-03-09 2003-10-22 뉴젠 테크놀로지스 인코포레이티드 Ｒｎａ 서열의 증폭을 위한 방법 및 조성물
US7122715B2 (en) * 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US6815167B2 (en) * 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
DE60324810D1 (de) 2002-09-20 2009-01-02 New England Biolabs Inc HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
EP1590470A4 (de) 2003-02-03 2006-09-27 Ge Healthcare Bio Sciences Cdna-amplifikation für expression-profiling
WO2005059157A2 (en) * 2003-12-11 2005-06-30 The Board Of Trustees Of The Leland Stanford Junior University METHODS AND COMPOSITIONS FOR USE IN PREPARING HAIRPIN RNAs
US20110059865A1 (en) * 2004-01-07 2011-03-10 Mark Edward Brennan Smith Modified Molecular Arrays
AU2005216549A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US20100075384A1 (en) * 2004-03-25 2010-03-25 New England Biolabs, Inc. Helicase-dependent amplification of circular nucleic acids
US8039210B2 (en) 2004-05-14 2011-10-18 The Johns Hopkins University Protein tyrosine phosphatase mutations in cancers
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7588894B2 (en) * 2005-02-01 2009-09-15 John Wayne Cancern Institute Use of ID4 for diagnosis and treatment of cancer
US7824890B2 (en) 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
ES2313143T3 (es) 2005-04-06 2009-03-01 Maurice Stroun Metodo para el diagnostico de cancer mediante la deteccion de adn y arn circulantes.
US9139849B2 (en) * 2005-04-08 2015-09-22 The United States of America as Represented by the Government of the Department of Health and Human Services Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
AU2006259565B2 (en) 2005-06-15 2011-01-06 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US8278035B2 (en) 2005-08-19 2012-10-02 Bioventures, Inc. Method and substances for isolating miRNAs
US7935310B2 (en) 2005-11-28 2011-05-03 Pacific Biosciences Of California, Inc. Uniform surfaces for hybrid material substrate and methods for making and using same
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
WO2007117832A2 (en) 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
EP2677039B8 (de) 2006-05-10 2022-10-05 DxTerity Diagnostics Incorporated Erkennung von Nukleinsäuretargets mithilfe chemisch reaktiver Oligonnucleotidsonden
US8768629B2 (en) 2009-02-11 2014-07-01 Caris Mpi, Inc. Molecular profiling of tumors
EP3260556B1 (de) 2006-05-31 2019-07-31 Sequenom, Inc. Verfahren zur nukleinsäureextraktion aus einer probe
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
AU2007203390B2 (en) 2007-04-26 2012-10-04 Yew Jin Fong Improved lace fastener
FR2924440B1 (fr) 2007-12-04 2015-01-09 Pf Medicament Nouveau procede de generation et de criblage d'une banque d'anticorps
EP2610352B1 (de) 2009-02-16 2014-10-08 Epicentre Technologies Corporation Vorlagenunabhängige Ligation von Einzelstrang-DNA
WO2010117817A2 (en) 2009-03-30 2010-10-14 Life Technologies Corporation Methods for generating target specific probes for solution based capture
CN104404134B (zh) 2009-04-03 2017-05-10 莱弗斯基因股份有限公司 多重核酸检测方法和系统
WO2010120803A2 (en) * 2009-04-13 2010-10-21 Somagenics Inc. Methods and compositions for detection of small rnas
GB0910302D0 (en) * 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
US20110237444A1 (en) 2009-11-20 2011-09-29 Life Technologies Corporation Methods of mapping genomic methylation patterns
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
WO2011120046A2 (en) 2010-03-26 2011-09-29 Swift Biosciences, Inc. Methods and compositions for isolating polynucleotides
EP2585593B1 (de) * 2010-06-24 2017-03-01 Population Genetics Technologies Ltd. Verfahren zur herstellung und verewigung einer polynukleotid-bibliothek sowie zur extraktion relevanter bereiche daraus
WO2012006291A2 (en) 2010-07-06 2012-01-12 Life Technologies Corporation Systems and methods to detect copy number variation
WO2012019320A1 (zh) 2010-08-11 2012-02-16 中国科学院心理研究所 一种甲基化dna的高通量测序方法及其应用
EP2426217A1 (de) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytische Verfahren für zellfreie Nucleinsäuren und Anwendungen
CN101985654A (zh) 2010-10-21 2011-03-16 徐州医学院 检测多样本多位点snp的检测方法
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
US9670527B2 (en) 2011-06-29 2017-06-06 The Johns Hopkins University Enrichment of nucleic acids by complimentary capture
EP2740804A4 (de) * 2011-08-05 2015-06-24 Toshiba Kk Verfahren für nukleinsäureanalyse
EP2746395B1 (de) 2011-09-08 2017-11-29 Kabushiki Kaisha DNAFORM Primer-set, verfahren zur amplifizierung einer nukleinsäuresequenz damit und verfahren zur erkennung von mutierter nukleinsäure damit
WO2013056178A2 (en) 2011-10-14 2013-04-18 Foundation Medicine, Inc. Novel estrogen receptor mutations and uses thereof
WO2013059740A1 (en) 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
WO2013074632A1 (en) 2011-11-14 2013-05-23 Rose Floyd D Mismatch nucleotide purification and identification
US20130217023A1 (en) 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
ES2828661T3 (es) 2012-03-20 2021-05-27 Univ Washington Through Its Center For Commercialization Métodos para reducir la tasa de error de la secuenciación de ADN masiva en paralelo mediante el uso de la secuenciación de secuencia consenso bicatenaria
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
CA2872141C (en) 2012-05-31 2016-01-19 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
WO2013181276A1 (en) 2012-06-01 2013-12-05 Fred Hutchinson Cancer Research Center Compositions and methods for detecting rare nucleic acid molecule mutations
JP2015521472A (ja) 2012-06-14 2015-07-30 フレッド ハチンソン キャンサー リサーチ センター 核酸分子における高感度変異検出のための組成物および方法
CN103998621A (zh) 2012-07-11 2014-08-20 北京贝瑞和康生物技术有限公司 片断dna检测方法、片断dna检测试剂盒及其应用
EP2875173B1 (de) 2012-07-17 2017-06-28 Counsyl, Inc. System und verfahren zur erkennung von genetischen variationen
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
WO2014014497A1 (en) 2012-07-20 2014-01-23 Verinata Health, Inc. Detecting and classifying copy number variation in a cancer genome
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20140066317A1 (en) 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CA2890004A1 (en) 2012-10-31 2014-05-08 Becton, Dickinson And Company Selective amplification and real-time pcr detection of rare mutations
WO2014113729A2 (en) 2013-01-18 2014-07-24 Foundation Mecicine, Inc. Methods of treating cholangiocarcinoma
US9290800B2 (en) * 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
CN113337604A (zh) 2013-03-15 2021-09-03 莱兰斯坦福初级大学评议会 循环核酸肿瘤标志物的鉴别和用途
US10081825B2 (en) * 2013-03-15 2018-09-25 Aegea Biotechnologies, Inc. Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
US10017807B2 (en) 2013-03-15 2018-07-10 Verinata Health, Inc. Generating cell-free DNA libraries directly from blood
US20150033372A1 (en) * 2013-05-01 2015-01-29 Kymab Limited Human VpreB & Chimaeric Surrogate Light Chains in Transgenic Non-Human Vertebrates
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9644232B2 (en) 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
US9499870B2 (en) 2013-09-27 2016-11-22 Natera, Inc. Cell free DNA diagnostic testing standards
CA2923812C (en) * 2013-10-17 2023-10-17 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
CA2929557C (en) 2013-11-07 2023-09-26 The Board Of Trustees Of The Leland Stanford Junior University Cell-free nucleic acids for the analysis of the human microbiome and components thereof
GB201321123D0 (en) 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
KR20240025725A (ko) 2013-12-11 2024-02-27 아큐라젠 홀딩스 리미티드 희귀 서열 변이를 검출하기 위한 조성물 및 방법
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
CN104745679B (zh) 2013-12-31 2018-06-15 杭州贝瑞和康基因诊断技术有限公司 一种无创检测egfr基因突变的方法及试剂盒
DK3604544T3 (en) * 2014-01-31 2022-02-14 Swift Biosciences Inc Improved methods for processing dna substrates
WO2015189636A1 (en) * 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
WO2016011280A1 (en) * 2014-07-16 2016-01-21 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
EP3201350B1 (de) 2014-09-30 2020-12-02 Global Life Sciences Solutions USA LLC Verfahren zur nukleinsäureanalyse direkt aus einer ungereinigten biologischen probe
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
MX2018004284A (es) 2015-10-09 2018-05-16 Accuragen Holdings Ltd Metodos y composiciones para el enriquecimiento de productos de amplificacion.
EP3383994A4 (de) 2015-12-03 2019-08-28 Accuragen Holdings Limited Verfahren und zusammensetzungen zum formen von ligationsprodukten
CN109511265B (zh) 2016-05-16 2023-07-14 安可济控股有限公司 通过链鉴定改进测序的方法
US10370701B2 (en) * 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
EP3475449B1 (de) 2016-06-23 2022-08-17 Accuragen Holdings Limited Verwendungen von zellfreie nukleinsäurestandards
WO2018035170A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems

Also Published As

Publication number Publication date
US20210002716A1 (en) 2021-01-07
CA2999902A1 (en) 2017-04-13
EP3359693A1 (de) 2018-08-15
CN108368545B (zh) 2022-05-17
IL258236B (en) 2021-12-01
US20180298434A1 (en) 2018-10-18
IL258236A (en) 2018-05-31
AU2016334233A1 (en) 2018-04-12
HK1259101A1 (zh) 2019-11-22
US10752942B2 (en) 2020-08-25
US20230287485A1 (en) 2023-09-14
JP2018537955A (ja) 2018-12-27
WO2017062863A1 (en) 2017-04-13
KR20180055905A (ko) 2018-05-25
CN114807323A (zh) 2022-07-29
EP3359693B1 (de) 2025-03-19
MX2018004284A (es) 2018-05-16
AU2016334233B2 (en) 2023-01-05
MX2024002873A (es) 2024-03-22
US11578359B2 (en) 2023-02-14
JP2022050563A (ja) 2022-03-30
JP2024099542A (ja) 2024-07-25
CN108368545A (zh) 2018-08-03
IL287586A (en) 2021-12-01
JP7008016B2 (ja) 2022-01-25
AU2023201912A1 (en) 2023-05-04
SG11201802864RA (en) 2018-05-30

Similar Documents

Publication Publication Date Title
IL287586A (en) Methods and preparations for enriching breeding products
EP3322297A4 (de) Freisetzungsverfahren und zusammensetzungen für nukleasevermitteltes genomengineering
EP3397260A4 (de) Verfahren und zusammensetzungen im zusammenhang mit chondrisomen aus blutprodukten
MA46427A (fr) Compositions d'oligonucléotides et procédés associés
EP3350315A4 (de) Verfahren zur autokatalytischen genombearbeitung und neutralisierung von autokatalytischer genombearbeitung sowie zusammensetzungen daraus
EP3493827A4 (de) Zusammensetzungen und verfahren für die immuntherapie
MA50636A (fr) Compositions et procédés pour inhiber l'activité d'arginase
EP3596213A4 (de) Zusammensetzungen und verfahren für erhöhte genexpression
EP3706784A4 (de) Zusammensetzungen und verfahren zur herstellung von t-zellen
EP3471745A4 (de) Verfahren und zusammensetzungen zur reduzierung von oxidativem stress
MA45270A (fr) Compositions d'oligonucléotides et procédés associés
EP3325017A4 (de) Oligonukleotidzusammensetzungen und verfahren dafür
EP3353309A4 (de) Zusammensetzungen und verfahren zur genomeditierung
EP3383994A4 (de) Verfahren und zusammensetzungen zum formen von ligationsprodukten
EP3331571A4 (de) Zusammensetzungen und verfahren zur behandlung von virusinfektionen
EP3635100A4 (de) Zusammensetzungen und verfahren zur expression von otoferlin
EP3289087A4 (de) Verfahren und zusammensetzungen zur herstellung von verkürzten maispflanzen
EP3289064A4 (de) Zusammensetzungen und verfahren zum nachweis von allergenen
EP3390642A4 (de) Zusammensetzungen und verfahren zur behandlung von retinitis pigmentosa 18 und retinitis pigmentosa 13
EP3370543A4 (de) Insektenprodukte und verfahren zur herstellung davon
EP3349743A4 (de) Verfahren und zusammensetzungen zur hemmung der dcn1-ubc12-interaktion
MA45272A (fr) Produits issus de liquide amniotique et procédés d'utilisation
EP3389673A4 (de) Verfahren und zusammensetzungen zur freisetzung von polynukleotiden
EP3423091A4 (de) Zusammensetzungen und verfahren zur induzierung von hiv-1-antikörpern
EP3380603A4 (de) Verfahren, medien und produkte zur kultivierung von embryonen

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20180327

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20190204

RIC1 Information provided on ipc code assigned before grant

Ipc: C12Q 1/68 20180101AFI20190129BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

17Q First examination report despatched

Effective date: 20190930

REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1259101

Country of ref document: HK

P01 Opt-out of the competence of the unified patent court (upc) registered

Effective date: 20230503

REG Reference to a national code

Ref country code: DE

Free format text: PREVIOUS MAIN CLASS: C12Q0001680000

Ref country code: DE

Ref legal event code: R079

Ref document number: 602016091632

Country of ref document: DE

Free format text: PREVIOUS MAIN CLASS: C12Q0001680000

Ipc: C12Q0001685300

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

RIC1 Information provided on ipc code assigned before grant

Ipc: C12Q 1/6858 20180101ALI20240214BHEP

Ipc: C12Q 1/6853 20180101AFI20240214BHEP

INTG Intention to grant announced

Effective date: 20240314

GRAJ Information related to disapproval of communication of intention to grant by the applicant or resumption of examination proceedings by the epo deleted

Free format text: ORIGINAL CODE: EPIDOSDIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

INTC Intention to grant announced (deleted)
GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

INTG Intention to grant announced

Effective date: 20241024

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE PATENT HAS BEEN GRANTED

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602016091632

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250619

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG9D

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250619

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250620

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

REG Reference to a national code

Ref country code: NL

Ref legal event code: MP

Effective date: 20250319

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 1777003

Country of ref document: AT

Kind code of ref document: T

Effective date: 20250319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250721

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250719

REG Reference to a national code

Ref country code: CH

Ref legal event code: U11

Free format text: ST27 STATUS EVENT CODE: U-0-0-U10-U11 (AS PROVIDED BY THE NATIONAL OFFICE)

Effective date: 20251101

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602016091632

Country of ref document: DE

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20251029

Year of fee payment: 10

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20251027

Year of fee payment: 10

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250319

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20251027

Year of fee payment: 10

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: CH

Payment date: 20251101

Year of fee payment: 10

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

REG Reference to a national code

Ref country code: CH

Ref legal event code: L10

Free format text: ST27 STATUS EVENT CODE: U-0-0-L10-L00 (AS PROVIDED BY THE NATIONAL OFFICE)

Effective date: 20260128

26N No opposition filed

Effective date: 20251222

REG Reference to a national code

Ref country code: HK

Ref legal event code: WD

Ref document number: 1259101

Country of ref document: HK