ES2031804T1 - Derivado del activador de plasminogeno de tejido. - Google Patents

Derivado del activador de plasminogeno de tejido.

Info

Publication number
ES2031804T1
ES2031804T1 ES199090102329T ES90102329T ES2031804T1 ES 2031804 T1 ES2031804 T1 ES 2031804T1 ES 199090102329 T ES199090102329 T ES 199090102329T ES 90102329 T ES90102329 T ES 90102329T ES 2031804 T1 ES2031804 T1 ES 2031804T1
Authority
ES
Spain
Prior art keywords
pct
protein
sec
amino acid
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES199090102329T
Other languages
English (en)
Other versions
ES2031804T3 (es
Inventor
Anne Dr. Rer Nat Stern
Ulrich Dr. Rer Nat. Kohnert
Rainer Dr. Rer. Nat. Rudolph
Stephan Dr. Rer. Nat. Fischer
Ulrich Dr. Med. Martin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of ES2031804T1 publication Critical patent/ES2031804T1/es
Application granted granted Critical
Publication of ES2031804T3 publication Critical patent/ES2031804T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Cosmetics (AREA)
  • Saccharide Compounds (AREA)

Abstract

EL INVENTO SE REFIERE A UN NUEVO DERIVADO DEL TEJIDO (T-PA) QUE SE EMPLEA ACTIVADOR DE PLASMINOGENO; NO ESTA GLICOSILADO, CONSISTE EN LA SECUENCIA DE AMINOACIDOS DE FORMULA (I); SE CODIFICA A TRAVES DE LA SECUENCIA DNA DE FORMULA (II) Y POSEE BUENAS PROPIEDADES PARA LA DISOLUCION DEL COAGULO DE SANGRE
ES90102329T 1989-02-07 1990-02-06 Derivado del activador de plasminogeno de tipo tisular. Expired - Lifetime ES2031804T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3903581A DE3903581A1 (de) 1989-02-07 1989-02-07 Gewebs-plasminogenaktivator-derivat

Publications (2)

Publication Number Publication Date
ES2031804T1 true ES2031804T1 (es) 1993-01-01
ES2031804T3 ES2031804T3 (es) 1995-11-01

Family

ID=6373566

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90102329T Expired - Lifetime ES2031804T3 (es) 1989-02-07 1990-02-06 Derivado del activador de plasminogeno de tipo tisular.

Country Status (29)

Country Link
US (1) US5223256A (es)
EP (1) EP0382174B1 (es)
JP (1) JP2559538B2 (es)
KR (1) KR970008485B1 (es)
AT (1) ATE126270T1 (es)
AU (1) AU623228B2 (es)
CA (1) CA2025900C (es)
CZ (1) CZ281836B6 (es)
DD (1) DD291779A5 (es)
DE (2) DE3903581A1 (es)
DK (1) DK0382174T3 (es)
ES (1) ES2031804T3 (es)
FI (1) FI100244B (es)
GR (2) GR900300140T1 (es)
HK (1) HK153396A (es)
HU (1) HU218092B (es)
IE (1) IE61077B1 (es)
IL (1) IL93280A (es)
LU (1) LU90025I2 (es)
LV (1) LV10302B (es)
NL (1) NL970008I2 (es)
NO (2) NO306563B1 (es)
NZ (1) NZ232337A (es)
PT (1) PT93082B (es)
RU (1) RU2107094C1 (es)
SK (1) SK55890A3 (es)
UA (1) UA27111A1 (es)
WO (1) WO1990009437A1 (es)
ZA (1) ZA90861B (es)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4634007A (en) * 1985-12-02 1987-01-06 Adolph Coors Company Carton blank with perforated tear line
US5676947A (en) * 1989-02-07 1997-10-14 Boehringer Manneheim Gmbh Method for treating thromboembolic conditions using thrombolytically active proteins
DE3923339A1 (de) * 1989-05-31 1990-12-06 Boehringer Mannheim Gmbh Gewebs-plasminogen-aktivator-derivat
GB9019120D0 (en) * 1990-09-01 1990-10-17 Beecham Group Plc Novel compounds
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DK0580685T3 (da) * 1991-04-16 1996-03-04 Boehringer Mannheim Gmbh Farmaceutisk emballageenhed indeholdende plasminogenaktivatorer til flerfoldig bolusindgift
DE4123845A1 (de) * 1991-07-18 1993-01-21 Boehringer Mannheim Gmbh Pharmazeutische verpackungseinheit enthaltend plasminogenaktivatoren zur mehrfachbolusgabe
US5510330A (en) * 1994-03-25 1996-04-23 Boehringer Mannheim Gmbh Combinations of thrombolytically active proteins and non-heparin anticoagulants, and uses thereof.
DE4423574A1 (de) * 1994-07-05 1996-01-11 Boehringer Mannheim Gmbh Nicht-glykosylierte Plasminogenaktivator-Derivate und ihre Verwendung bei erhöhtem Blutungsrisiko
AU4259496A (en) * 1994-12-06 1996-06-26 Boehringer Mannheim Gmbh Use of the protease domain of human plasminogen activator for the treatment of thromboembolic diseases
US5723122A (en) * 1995-06-01 1998-03-03 Boehringer Mannheim Gmbh Use of the protease domain of human plasminogen activator for the treatment of thromboembolic diseases
AU2660397A (en) * 1996-04-05 1997-10-29 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
PT1000167E (pt) 1997-07-23 2006-05-31 Roche Diagnostics Gmbh Producao de proteinas mutantes humanas em celulas humanas por recombinacao homologa
WO1999009185A1 (de) * 1997-08-13 1999-02-25 Roche Diagnostics Gmbh Plasminogenaktivator mit verbesserter zymogenität
WO1999009184A1 (de) * 1997-08-13 1999-02-25 Roche Diagnostics Gmbh Plasminogenaktivator mit verbesserter zymogenität und verminderter fibrinbindung
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
DE10153601A1 (de) * 2001-11-02 2003-05-22 Paion Gmbh DSPA zur Behandlung von Schlaganfall
RU2323001C1 (ru) * 2007-05-30 2008-04-27 Общество с ограниченной ответственностью "Научно-производственное предприятие "Техноген" Фармацевтическая композиция, обладающая фибринолитическим действием
AR068914A1 (es) * 2007-10-18 2009-12-16 Paion Deutschland Gmbh Uso de un activador de plasminogeno para la preparacion de un medicamento para el tratamiento de apoplejia
EP2289541A1 (en) 2009-08-31 2011-03-02 PAION Deutschland GmbH Treatment of neurological or neurodegenerative disorders
EP2289542A1 (en) 2009-08-31 2011-03-02 PAION Deutschland GmbH Treatment of neurological or neurodegenerative disorders
JP7360945B2 (ja) 2017-02-03 2023-10-13 アクティコー バイオテック 抗ヒトｇｐｖｉ抗体を用いた血小板凝集の阻害
WO2018210860A1 (en) 2017-05-16 2018-11-22 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and pharmaceutical compositions for the treatment of acute ischemic stroke
US12123000B2 (en) 2018-06-15 2024-10-22 Inserm (Institut National De La Sante Et De La Recherche Medicale) Use of PI3KC2B inhibitors for the preservation of vascular endothelial cell barrier integrity
WO2021148439A1 (en) 2020-01-21 2021-07-29 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for stimulating cerebrovascular function
EP4161579A1 (en) 2020-06-09 2023-04-12 Institut National de la Santé et de la Recherche Médicale (INSERM) Fucoidan-functionalized polysaccharide particles with t-pa for targeted thrombolytic therapy
EP4482863A1 (en) 2022-02-21 2025-01-01 Acticor Biotech Treatment of cardiovascular diseases using anti-human gpvi antibodies
EP4556492A1 (en) 2023-11-14 2025-05-21 Acticor Biotech Treatment of cardiovascular diseases using anti-human gpvi antibodies in subpopulations of subjects

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766075A (en) * 1982-07-14 1988-08-23 Genentech, Inc. Human tissue plasminogen activator
EP0112122B1 (en) * 1982-12-14 1991-08-28 South African Inventions Development Corporation Plasminogen activator
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4620948A (en) * 1982-12-22 1986-11-04 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4753879A (en) * 1984-08-27 1988-06-28 Biogen N.V. Modified tissue plasminogen activators
US5073494A (en) * 1985-04-22 1991-12-17 Genentech, Inc. Human tissue plasminogen activator substituted at position 275 or at positions 275 and 277
JPH0672105B2 (ja) * 1985-10-02 1994-09-14 持田製薬株式会社 血栓溶解剤及びその製法
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US5034225A (en) * 1985-12-17 1991-07-23 Genentech Inc. Stabilized human tissue plasminogen activator compositions
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
DE3611817A1 (de) * 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen
DE3643158A1 (de) * 1986-04-21 1987-11-19 Boehringer Mannheim Gmbh Gewebs-plasminogen-aktivator(tpa) - derivat und seine herstellung
US4898825A (en) * 1986-05-07 1990-02-06 Mitsui Toatsu Chemicals, Incorporated Methods for purification of single-chain and double-chain tissue plasminogen activator
JPH0779692B2 (ja) * 1986-05-07 1995-08-30 三井東圧化学株式会社 一本鎖ｔ−ＰＡと二本鎖ｔ−ＰＡを分離する方法
JPS6463378A (en) * 1986-08-11 1989-03-09 Mitsui Toatsu Chemicals Separation of single stranded tpa and double standard tpa
SE8702562L (sv) * 1987-06-18 1988-12-19 Kabigen Ab Nya fibrinolytiska enzymer
US4935237A (en) * 1988-03-21 1990-06-19 Genentech, Inc. Processes for the preparation of t-PA mutants
IL87276A (en) * 1987-08-03 1995-07-31 Fujisawa Pharmaceutical Co Analog of tissue plasminogen activator comprising only the kringle 2 and protease domain dna encoding the same processes for the preparation thereof and pharmaceutical compositions containing the same
US5037752A (en) * 1987-10-09 1991-08-06 Monsanto Company Modified tissue plasminogen activator substituted at cysteine-73 and lysine-277
US4963357A (en) * 1987-10-09 1990-10-16 Monsanto Company Tissue plasminogen activator modified by the substitution of Arg for Cys at position 73, methods and pharmaceutical compositions therefor

Also Published As

Publication number Publication date
JPH03500724A (ja) 1991-02-21
CA2025900C (en) 1999-01-19
AU623228B2 (en) 1992-05-07
FI904951A0 (fi) 1990-10-08
JP2559538B2 (ja) 1996-12-04
UA27111A1 (uk) 2000-02-28
HK153396A (en) 1996-08-16
KR910700336A (ko) 1991-03-14
NZ232337A (en) 1991-09-25
DD291779A5 (de) 1991-07-11
HUT58813A (en) 1992-03-30
SK279029B6 (sk) 1998-05-06
WO1990009437A1 (de) 1990-08-23
PT93082B (pt) 1996-03-29
IL93280A0 (en) 1990-11-29
GR3017099T3 (en) 1995-11-30
LV10302A (lv) 1994-10-20
ZA90861B (en) 1990-11-28
NL970008I1 (nl) 1997-04-01
HU218092B (hu) 2000-05-28
IL93280A (en) 1995-08-31
KR970008485B1 (ko) 1997-05-24
ATE126270T1 (de) 1995-08-15
NO904211D0 (no) 1990-09-27
EP0382174B1 (de) 1995-08-09
FI100244B (fi) 1997-10-31
IE900343L (en) 1990-08-07
NL970008I2 (nl) 1997-07-01
NO306563B1 (no) 1999-11-22
IE61077B1 (en) 1994-09-21
CZ281836B6 (cs) 1997-02-12
RU2107094C1 (ru) 1998-03-20
NO904211L (no) 1990-09-27
GR900300140T1 (en) 1991-09-27
DE3903581A1 (de) 1990-08-16
EP0382174A1 (de) 1990-08-16
DE59009487D1 (de) 1995-09-14
SK55890A3 (en) 1998-05-06
CA2025900A1 (en) 1990-08-08
HU901644D0 (en) 1991-04-29
CZ55890A3 (en) 1996-10-16
DK0382174T3 (da) 1996-01-02
LV10302B (en) 1995-04-20
US5223256A (en) 1993-06-29
AU5047090A (en) 1990-09-05
PT93082A (pt) 1990-08-31
ES2031804T3 (es) 1995-11-01
NO2000002I1 (no) 2000-05-19
LU90025I2 (fr) 1997-04-22

Similar Documents

Publication Publication Date Title
ATE126270T1 (de) Gewebs-plasminogenaktivator-derivat.
ATE189611T1 (de) Zusammensetzungen, die eine blutgerinnselpolymermatrix zur verabreichung von osteogenen proteinen enthalten
FR2591485B1 (fr) Compositions stabilisees contenant l'activateur du plasminogene du tissu humain.
EA200000378A1 (ru) Усеченные по амино-концу мср-2 как антагонисты хемокинов
BR9007115A (pt) Novo trombolitico
ES2108694T3 (es) Inhibidor de proteasa.
DK274990A (da) Vaevsplasminogenaktivator-(t-pa)-varianter, dma-sekvenser, som koder for disse, replicerbare udtrykkelsesvektorer for dna-sekvenserne, eukaryote vaertsceller transformeret med vektorerne og farmaceutiske praeparater indeholdende t-pa-varianterne
ES2073391T3 (es) Metodo y composiciones terapeuticas para el tratamiento de trastornos de perdidas de sangre.
DK0471718T3 (da) Paracetamolholdigt farmaceutisk præparat
DK0542869T3 (da) Vævsplasminogenaktivatorvarianter med nedsat udskillelse
ATE103178T1 (de) T-pa pro stabilisierung.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 382174

Country of ref document: ES