ES2038966T3 - Expresion y secrecion de proteinas heterologas mediante transformantes de yarrowia lipolytica. - Google Patents

Expresion y secrecion de proteinas heterologas mediante transformantes de yarrowia lipolytica.

Info

Publication number
ES2038966T3
ES2038966T3 ES198686307839T ES86307839T ES2038966T3 ES 2038966 T3 ES2038966 T3 ES 2038966T3 ES 198686307839 T ES198686307839 T ES 198686307839T ES 86307839 T ES86307839 T ES 86307839T ES 2038966 T3 ES2038966 T3 ES 2038966T3
Authority
ES
Spain
Prior art keywords
heterological
yarrowia lipolytica
secretion
xpr2
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198686307839T
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English (en)
Inventor
Lance Steven Davidow
Arthur Ernest Franke
John Robert Dezeeuw
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Pfizer Inc
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Pfizer Inc
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Publication date
Application filed by Pfizer Inc filed Critical Pfizer Inc
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Publication of ES2038966T3 publication Critical patent/ES2038966T3/es
Anticipated expiration legal-status Critical
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/472—Complement proteins, e.g. anaphylatoxin, C3a, C5a
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatment Of Sludge (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Magnetic Ceramics (AREA)
  • Peptides Or Proteins (AREA)
  • Paper (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Fodder In General (AREA)
  • Noodles (AREA)
  • Measurement Of Velocity Or Position Using Acoustic Or Ultrasonic Waves (AREA)

Abstract

SECUENCIA DE LOS GENES XPR2 Y LEV2 DE YARROWIA LIPOLYTICA, VEHICULOS DE CLONACION DE YARROWIA LIPOLYTICA RECOMBINANTE QUE CONPRENDA LA CODIFICACION DE ADN HETEROLOGO PARA LA EXPRESION DE PROTEINAS DE MAMIFEROS Y OTROS POLIPEPTIDOS, QUE INCLUYEN PLASMIDOS ADECUADOS PARA LA TRANSFORMACION DE HUESPEDES DE Y.LIPOLYTICA E INCORPORAN UN HOMOLOGO DE REGULON AL HUESPED EDN SU ESTADO NO TRANSFORMADO Y SEÑALES DE SECRECION EN FASE DE LECTURA CON LA SECUENCIA CODIFICADORA DE LA PROTEINA CORRESPONDIENTE AL GEN HETEROLOGO; VECTORES DE EXPRESION INTEGRATIVA QUE UTILIZAN PROMOTOR GENICO XPR2, PREPROREGION DE PROTEASA ALCALINA Y REGION DEL TERMINADOR XPR2 Y LAS QUE TIENE EL PROMOTOR LEV2 Y SECUENCIAS DE SEÑALES SECRETORAS DE PROTEASA ALCALINA, CAPACES, EN UN CULTIVO CELULAR DE Y. LIPOLYTICA TRANSFORMADO, DE EXPRESAR Y SECRETAR UNA PROTEINA HETEROLOGA FUERA DE LA CELULA; TRANSFORMANTES DE Y. L. QUE COMPRENDEN DICHOS VECTORES Y PLASMIDOS; METODOS PARA PREPARAR VECTORES PARA LA SECRECION DIRECTA DE PROTEINASHETEROLOGAS ESPECIFICAS CODIFICADAS POR GENES, ADNC O ADN SINTETICO EN LA Y. L. EN SU ESTADO MADURO Y FUNCIONAL.
ES198686307839T 1985-10-18 1986-10-10 Expresion y secrecion de proteinas heterologas mediante transformantes de yarrowia lipolytica. Expired - Lifetime ES2038966T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US78920685A 1985-10-18 1985-10-18
US06/841,121 US4937189A (en) 1985-10-18 1986-03-18 Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants

Publications (1)

Publication Number Publication Date
ES2038966T3 true ES2038966T3 (es) 1993-08-16

Family

ID=27120892

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198686307839T Expired - Lifetime ES2038966T3 (es) 1985-10-18 1986-10-10 Expresion y secrecion de proteinas heterologas mediante transformantes de yarrowia lipolytica.

Country Status (24)

Country Link
US (1) US4937189A (es)
EP (1) EP0220864B1 (es)
JP (1) JP3096297B2 (es)
KR (1) KR900008950B1 (es)
CN (3) CN1034428C (es)
AT (1) ATE86302T1 (es)
AU (1) AU586041B2 (es)
CA (1) CA1340837C (es)
DD (7) DD269396A5 (es)
DE (1) DE3687878T2 (es)
DK (1) DK496886A (es)
EG (1) EG18142A (es)
ES (1) ES2038966T3 (es)
FI (1) FI90352C (es)
HU (1) HU209150B (es)
IE (1) IE59774B1 (es)
IL (7) IL99894A (es)
NO (2) NO177318C (es)
NZ (1) NZ217985A (es)
PH (3) PH27026A (es)
PL (2) PL154427B1 (es)
PT (1) PT83562B (es)
RU (2) RU2157845C2 (es)
YU (2) YU46412B (es)

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CA2729626C (fr) * 2008-07-11 2017-12-12 Jean-Marc Nicaud Nouvelles souches de levure mutantes capables d'accumuler une grande quantite de lipides
RU2388823C1 (ru) * 2008-08-21 2010-05-10 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП ГосНИИгенетика) Интегративный плазмидный вектор для экспрессии генов в дрожжах
FR2962133B1 (fr) 2010-07-01 2014-09-12 Agronomique Inst Nat Rech Optimisation de la synthese et de l'accumulation de lipides
JP2014503216A (ja) * 2010-12-30 2014-02-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー スクロース利用のための、ヤロウィア・リポリティカ（Ｙａｒｒｏｗｉａｌｉｐｏｌｙｔｉｃａ）におけるサッカロミセス・セレビシア（Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ）ＳＵＣ２遺伝子の使用
EP2691518A1 (en) 2011-03-31 2014-02-05 E.I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
WO2012138613A1 (en) 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
WO2012138612A1 (en) 2011-04-07 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-coa reductase promoter regions for gene expression in yeast
GB201106188D0 (en) * 2011-04-12 2011-05-25 Csir Production of extracellular polypeptides
FR3002774A1 (fr) 2013-03-04 2014-09-05 Agronomique Inst Nat Rech Levures mutantes ayant une production accrue de lipides et d'acide citrique
FR3005317B1 (fr) 2013-05-02 2016-03-18 Agronomique Inst Nat Rech Levures mutantes capables de produire un acide gras inhabituel
US10611807B2 (en) 2014-01-31 2020-04-07 Dsm Ip Assets B.V. Promoters suitable for heterologous gene expression in yeast
EP3155094A1 (en) 2014-06-11 2017-04-19 Institut National De La Recherche Agronomique (INRA) Improved lipid accumulation in yarrowia lipolytica strains by overexpression of hexokinase and new strains thereof
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica
WO2016075314A1 (fr) 2014-11-13 2016-05-19 Institut National De La Recherche Agronomique Identification de facteurs de transcription de yarrowia lipolytica affectant la production de proteines
DK3387134T3 (da) 2015-12-11 2020-12-21 Danisco Us Inc Fremgangsmåder og sammensætninger til øget nukleasemedieret genommodifikation og reducerede virkninger uden for målstedet
DK3390631T3 (da) 2015-12-18 2020-07-13 Danisco Us Inc Fremgangsmåder og sammensætninger til t-rna-baseret guide-rna-ekspression
US20190136278A1 (en) 2016-05-10 2019-05-09 Institut National De La Recherche Agronomique Mutant yeast strains with enhanced production of erythritol or erythrulose
EP3348647A1 (en) 2017-01-13 2018-07-18 Institut National De La Recherche Agronomique Mutant yeast strain capable of producing medium chain fatty acids

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Also Published As

Publication number Publication date
DD267996A5 (de) 1989-05-17
CN1069346C (zh) 2001-08-08
IL99894A (en) 1993-02-21
DE3687878T2 (de) 1993-07-15
DD267997A5 (de) 1989-05-17
CN1141339A (zh) 1997-01-29
PT83562B (pt) 1989-05-31
IE59774B1 (en) 1994-04-06
IL99893A0 (en) 1992-08-18
NO923616D0 (no) 1992-09-17
DD269397A5 (de) 1989-06-28
IL80297A0 (en) 1987-01-30
FI90352C (fi) 1994-01-25
IL99894A0 (en) 1992-08-18
DD267995A5 (de) 1989-05-17
DK496886A (da) 1987-06-11
FI864206A7 (fi) 1987-04-19
FI90352B (fi) 1993-10-15
NO864148L (no) 1987-04-21
DK496886D0 (da) 1986-10-17
YU46571B (sh) 1993-11-16
NO177318C (no) 1995-08-23
PH27026A (en) 1993-02-01
PH27171A (en) 1993-04-02
PL155850B1 (en) 1992-01-31
HU209150B (en) 1994-03-28
YU46412B (sh) 1993-10-20
NO923616L (no) 1987-04-21
YU124588A (en) 1989-08-31
IL99893A (en) 1993-02-21
US4937189A (en) 1990-06-26
CN86106522A (zh) 1987-09-16
IE862754L (en) 1987-04-18
RU2157845C2 (ru) 2000-10-20
PT83562A (en) 1986-11-01
CN1138632A (zh) 1996-12-25
PH27198A (en) 1993-04-16
AU586041B2 (en) 1989-06-29
DD267998A5 (de) 1989-05-17
IL99892A (en) 1993-02-21
NO178793C (no) 1996-06-05
ATE86302T1 (de) 1993-03-15
PL154427B1 (en) 1991-08-30
KR870004138A (ko) 1987-05-07
CN1034428C (zh) 1997-04-02
KR900008950B1 (ko) 1990-12-15
JP3096297B2 (ja) 2000-10-10
YU177386A (en) 1989-04-30
EG18142A (en) 1992-08-30
NO177318B (no) 1995-05-15
JPS63164889A (ja) 1988-07-08
HUT43632A (en) 1987-11-30
NO864148D0 (no) 1986-10-17
AU6416186A (en) 1987-05-14
CA1340837C (en) 1999-12-07
EP0220864B1 (en) 1993-03-03
NO178793B (no) 1996-02-26
IL80297A (en) 1993-02-21
NZ217985A (en) 1991-06-25
DD269396A5 (de) 1989-06-28
PL261912A1 (en) 1987-10-19
RU2069694C1 (ru) 1996-11-27
IL99892A0 (en) 1992-08-18
DE3687878D1 (de) 1993-04-08
DD276886A5 (de) 1990-03-14
FI864206A0 (fi) 1986-10-17
EP0220864A1 (en) 1987-05-06

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