ES2065318T3 - Sustancia que se une a trombina y procedimiento para su preparacion. - Google Patents

Sustancia que se une a trombina y procedimiento para su preparacion.

Info

Publication number
ES2065318T3
ES2065318T3 ES87110005T ES87110005T ES2065318T3 ES 2065318 T3 ES2065318 T3 ES 2065318T3 ES 87110005 T ES87110005 T ES 87110005T ES 87110005 T ES87110005 T ES 87110005T ES 2065318 T3 ES2065318 T3 ES 2065318T3
Authority
ES
Spain
Prior art keywords
thrombin
condition
isoelectric point
molecular weight
sds page
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87110005T
Other languages
English (en)
Inventor
Nobuo Aoki
Shigeru Kimura
Masami Shiratsuchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kowa Co Ltd
Original Assignee
Kowa Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP61172626A external-priority patent/JPS6330423A/ja
Application filed by Kowa Co Ltd filed Critical Kowa Co Ltd
Application granted granted Critical
Publication of ES2065318T3 publication Critical patent/ES2065318T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/7455—Thrombomodulin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/834—Urine; urinary system

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

SE OBTIENEN SUSTANCIAS QUE SE UNEN A LA TROMBINA FRACCIONANDO ORINA HUMANA POR CROMATOGRAFIA DE INTERCAMBIO IONICO, CROMATOGRAFIA DE AFINIDAD USANDO UN SOPORTE UNIDO A TROMBINA, CROMATOGRAFIA DE INMUNOADSORCION EN COLUMNA, FILTRACION EN GEL O FRACCIONAMIENTO POR PESOS MOLECULARES. UNA DE LAS SUSTANCIAS TIENE UN PESO MOLECULAR DE 46.500 +- 6.000 EN ESTADO REDUCIDO Y 39.000 +-10.000 EN ESTADO NO REDUCIDO POR SDS PAGE Y UN PUNTO ISOELECTRICO A PH ENTRE 5,0 Y 5,3, MIENTRAS QUE LA OTRA TIENE UN PESO MOLECULAR DE 40.000+-8.000 EN ESTADO REDUCIDO Y DE 31.000+-10.000 EN ESTADO NO REDUCIDO POR SDS PAGE Y UN PUNTO ISOELECTRICO A PH ENTRE 4,9 Y 5,7. LAS SUSTANCIAS TIENEN UNA FUERTE AFINIDAD HACIA LA TROMBINA. SON CAPACES DE PROMOVER LA ACTIVACION CATALIZADA POR TROMBINA DE PROTEINA C Y UN TIEMPO DE COAGULACION PROLONGADO. SON ESTABLES FRENTE A LOS AGENTES DESNATURALIZANTES (UREA Y DODECILSULFATO SODICO).
ES87110005T 1986-07-15 1987-07-10 Sustancia que se une a trombina y procedimiento para su preparacion. Expired - Lifetime ES2065318T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP16608486 1986-07-15
JP61172626A JPS6330423A (ja) 1986-07-22 1986-07-22 トロンビン結合性物質およびその製法

Publications (1)

Publication Number Publication Date
ES2065318T3 true ES2065318T3 (es) 1995-02-16

Family

ID=26490590

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87110005T Expired - Lifetime ES2065318T3 (es) 1986-07-15 1987-07-10 Sustancia que se une a trombina y procedimiento para su preparacion.

Country Status (6)

Country Link
US (1) US5047503A (es)
EP (1) EP0253331B1 (es)
KR (1) KR890002388A (es)
AT (1) ATE113062T1 (es)
DE (1) DE3750664T2 (es)
ES (1) ES2065318T3 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3876124T2 (de) * 1987-04-01 1993-05-06 Mitsubishi Gas Chemical Co Immunoassay fuer thrombomodulin.
JPS6445398A (en) * 1987-08-13 1989-02-17 Kowa Co Antithrombin-binding substance monoclonal antibody, hybridoma producing said antibody and method for purifying and measuring thrombin-binding substance utilizing said monoclonal antibody
JPH0720997B2 (ja) * 1988-08-29 1995-03-08 興和株式会社 新規なトロンビン結合性物質及びその製法
US5202421A (en) * 1988-12-27 1993-04-13 Mochida Pharmaceutical Co., Ltd. Anticoagulant substance obtained from urine and process for the preparation thereof
US6063763A (en) * 1989-04-28 2000-05-16 Schering Aktiengesellschaft Protease-resistant thrombomodulin analogs
AU643306B2 (en) * 1990-06-27 1993-11-11 Mochida Pharmaceutical Co., Ltd. Anticoagulant polypeptides
US5492834A (en) * 1993-07-09 1996-02-20 Beckman Instruments, Inc. Method of sample preparation for urine protein analysis with capillary electrophoresis
AU2003210802B2 (en) * 2002-02-05 2009-09-10 Genentech Inc. Protein purification

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2720704C2 (de) * 1977-05-07 1986-09-25 Behringwerke Ag, 3550 Marburg Neues Glycoprotein, Verfahren zu seiner Herstellung und seine Verwendung
CH643297A5 (de) * 1978-06-30 1984-05-30 Alpha Patent Ltd Verfahren zur reindarstellung von urokinase.
US4230697A (en) * 1978-07-03 1980-10-28 Morinaga Milk Industry Co. Ltd. Virus-inactivated HGI-glycoprotein capable of stimulating proliferation and differentiation of human granulocyte, process for preparing same and leukopenia curative containing same
DE2842467A1 (de) * 1978-09-29 1980-04-10 Behringwerke Ag Neues ubiquitaeres gewebsprotein pp tief 8
GB2038337B (en) * 1978-12-21 1983-01-19 Green Cross Corp Process which comprises separation of urokinases having different molecular weights
DE3013724A1 (de) * 1980-04-10 1981-10-15 Behringwerke Ag, 3550 Marburg Neues protein pp (pfeil abwaerts)9(pfeil abwaerts), verfahren zu seiner anreicherung und gewinnung sowieseine verwendung
DE3315000A1 (de) * 1983-04-26 1984-10-31 Behringwerke Ag, 3550 Marburg Gewebeprotein pp(pfeil abwaerts)4(pfeil abwaerts), verfahren zu seiner gewinnung sowie seine verwendung
DE3410694A1 (de) * 1984-03-23 1985-10-03 Behringwerke Ag, 3550 Marburg Gewebeprotein pp(pfeil abwaerts)2(pfeil abwaerts)(pfeil abwaerts)1(pfeil abwaerts), verfahren zu seiner gewinnung sowie seine verwendung
JPS60199819A (ja) * 1984-03-23 1985-10-09 Kowa Co トロンビン結合性物質およびその製法
US4708948A (en) * 1984-04-20 1987-11-24 The United States Of America As Represented By The Department Of Health And Human Services Substantially purified tumor growth inhibitory factor
KR930007434B1 (ko) * 1984-09-21 1993-08-10 베링거 잉겔하임 인터내셔날 게엠베하 혈액응고 억제 단백질 제조방법
US4677195A (en) * 1985-01-11 1987-06-30 Genetics Institute, Inc. Method for the purification of erythropoietin and erythropoietin compositions
CA1265446A (en) * 1985-09-30 1990-02-06 Masahiro Maki Anticoagulating substance, process for preparing same and anticoagulant comprising same as an effective component
JPH0689014B2 (ja) * 1986-01-21 1994-11-09 興和株式会社 トロンビン結合性物質およびその製法

Also Published As

Publication number Publication date
ATE113062T1 (de) 1994-11-15
DE3750664T2 (de) 1995-06-22
EP0253331A2 (en) 1988-01-20
KR890002388A (ko) 1989-04-10
EP0253331A3 (en) 1989-10-11
EP0253331B1 (en) 1994-10-19
DE3750664D1 (de) 1994-11-24
US5047503A (en) 1991-09-10

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