ES2085245T3 - Procedimiento de purificacion de acidos nucleicos. - Google Patents

Procedimiento de purificacion de acidos nucleicos.

Info

Publication number
ES2085245T3
ES2085245T3 ES90200678T ES90200678T ES2085245T3 ES 2085245 T3 ES2085245 T3 ES 2085245T3 ES 90200678 T ES90200678 T ES 90200678T ES 90200678 T ES90200678 T ES 90200678T ES 2085245 T3 ES2085245 T3 ES 2085245T3
Authority
ES
Spain
Prior art keywords
nucleic acid
purification procedure
acid purification
relates
isolating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90200678T
Other languages
English (en)
Other versions
ES2085245T1 (es
ES2085245T5 (es
Inventor
Willem Rene Boom
Henriette Maria Alei Adriaanse
Tim Kievits
Peter Franklin Lens
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Akzo Nobel NV
Original Assignee
Akzo Nobel NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=19854348&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2085245(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Akzo Nobel NV filed Critical Akzo Nobel NV
Publication of ES2085245T1 publication Critical patent/ES2085245T1/es
Publication of ES2085245T3 publication Critical patent/ES2085245T3/es
Application granted granted Critical
Publication of ES2085245T5 publication Critical patent/ES2085245T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Hard Magnetic Materials (AREA)

Abstract

Un proceso para aislar ácido nucleico a partir de un material de partida biológico complejo que contenga ácido nucleico, caracterizado por la mezcla del material de partida biológico complejo, una sustancia caotrópica y una fase sólida que se une al ácido nucleico conteniendo sílice o un derivado de la misma, separando la fase sólida con el ácido nucleico unido a ella del líquido, después de lo cual los complejos fase sólida-ácido nucleico así obtenidos son lavados y, si se requiere, el ácido nucleico es eluido de dichos complejos, donde se selecciona el material de partida biológica de: sangre completa, suero sanguíneo, capa amarillenta, orina, heces, líquido cerebroespinal, esperma, saliva, tejidos y cultivos celulares, productos alimenticios, vacunas, leche infectada con un virus o una bacteria, material vegetal, bacterias gram positivas, levaduras, mohos, fluidos corporales y material biológico posiblemente infectado con virus o bacterias.
ES90200678T 1989-03-23 1990-03-22 Procedimiento de purificacion de acidos nucleicos. Expired - Lifetime ES2085245T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL8900725 1989-03-23
NL8900725A NL8900725A (nl) 1989-03-23 1989-03-23 Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.

Publications (3)

Publication Number Publication Date
ES2085245T1 ES2085245T1 (es) 1996-06-01
ES2085245T3 true ES2085245T3 (es) 1997-11-01
ES2085245T5 ES2085245T5 (es) 2007-06-01

Family

ID=19854348

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90200678T Expired - Lifetime ES2085245T5 (es) 1989-03-23 1990-03-22 Procedimiento de purificacion de acidos nucleicos.

Country Status (12)

Country Link
EP (2) EP0389063B2 (es)
JP (3) JP2680462B2 (es)
KR (1) KR0148693B1 (es)
AT (1) ATE156830T1 (es)
AU (1) AU641641B2 (es)
CA (2) CA2271603A1 (es)
DE (2) DE389063T1 (es)
DK (1) DK0389063T4 (es)
ES (1) ES2085245T5 (es)
GR (2) GR960300019T1 (es)
NL (1) NL8900725A (es)
ZA (1) ZA902190B (es)

Families Citing this family (185)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992018514A1 (en) * 1991-04-12 1992-10-29 Minnesota Mining And Manufacturing Company Purification of nucleic acids using metal oxide supports
US5329000A (en) * 1991-10-31 1994-07-12 Becton, Dickinson And Company Purification of DNA with silicon tetrahydrazide
DE4143639C2 (de) * 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
AU7375794A (en) * 1993-07-28 1995-02-28 Akzo Nobel N.V. Process for isolating nucleic acid from gram positive microorganisms
JP3451667B2 (ja) * 1993-08-24 2003-09-29 東ソー株式会社 核酸抽出及び特定核酸配列の検出方法
JP3696238B2 (ja) * 1993-08-30 2005-09-14 プロメガ・コーポレイシヨン 核酸精製用組成物及び方法
US6043032A (en) * 1993-09-22 2000-03-28 Tosoh Corporation Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
GB2282138B (en) * 1993-09-28 1997-09-03 Tosoh Corp Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
GB9323305D0 (en) * 1993-11-11 1994-01-05 Medinnova Sf Isoaltion of nucleic acid
EP0743950B1 (de) * 1994-02-11 2001-12-12 QIAGEN GmbH Verfahren zur trennung von doppelstrang/einzelstrangnukleinsäurestrukturen
DE59506735D1 (de) * 1994-06-14 1999-10-07 Invitek Gmbh Universelles verfahren zur isolierung und reinigung von nukleinsäuren aus extrem geringen mengen sowie sehr stark verunreinigten unterschiedlichsten ausgangsmaterialien
DE4420732A1 (de) 1994-06-15 1995-12-21 Boehringer Mannheim Gmbh Vorrichtung zur Behandlung von Nukleinsäuren aus einer Probe
DE4420900A1 (de) 1994-06-15 1995-12-21 Boehringer Mannheim Gmbh Verfahren zur mehrmaligen Entnahme von Flüssigkeiten
JPH10504834A (ja) * 1994-08-29 1998-05-12 アクゾ・ノベル・エヌ・ベー 核酸などの生物材料の単離に使用する装置
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
NL9402122A (nl) 1994-12-14 1996-07-01 Univ Nijmegen Werkwijze voor het bereiden van aan kunststof gekoppelde niet-eiwitachtige macrobiomoleculen welke sterk negatief geladen zijn, aan kunststof gekoppelde negatief geladen niet-eiwitachtige macrobiomoleculen alsmede een microtiterplaat waarvan de putjes zijn bekleed met negatief geladen niet-eiwitachtige macrobiomoleculen.
US5576196A (en) * 1995-01-13 1996-11-19 Vical Incorporated Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US6919175B1 (en) 1995-04-01 2005-07-19 Roche Diagnostics Gmbh System for releasing and isolating nucleic acids
DE19512368A1 (de) * 1995-04-01 1996-10-02 Boehringer Mannheim Gmbh System zur Freisetzung und Isolierung von Nukleinsäuren
KR100463475B1 (ko) 1995-06-08 2005-06-22 로셰 디아그노스틱스 게엠베하 자기성피그먼트
DE19520398B4 (de) * 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
DE69636553T2 (de) * 1995-07-07 2007-05-31 Toyo Boseki K.K. Nukleinsäuren bindender magnetischer Träger und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
DE19530132C2 (de) * 1995-08-16 1998-07-16 Max Planck Gesellschaft Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
US5783686A (en) * 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
ATE284891T1 (de) * 1996-02-14 2005-01-15 Biomerieux Bv Isolierung von einzelsträngigen nukleinsäuren
DE19607202A1 (de) * 1996-02-26 1997-08-28 Uwe Dr Michel Reagenzienkit zur Präparation von Nukleinsäuren
NL1002781C1 (nl) * 1996-04-03 1997-10-06 Amsterdam Support Diagnostics Isolatie en/of amplificatie van hepatitis-C-virus-(HCV) -nucleïnezuren uit monsters waarvan vermoed wordt dat zij HCV bevatten.
EP0814156B1 (en) * 1996-06-18 2003-03-05 The Institute Of Physical & Chemical Research Method for the purification of DNA
DE69734263T2 (de) * 1996-07-12 2006-07-13 Toyo Boseki K.K. Verfahren zur Isolierung von Ribonucleinsäuren.
US5763185A (en) * 1996-12-30 1998-06-09 Becton Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
US6210881B1 (en) 1996-12-30 2001-04-03 Becton, Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
GB9709728D0 (en) 1997-05-13 1997-07-02 Dynal As Single step method
WO1999000168A1 (en) * 1997-06-27 1999-01-07 Life Technologies, Inc. One step device and process for concentration and purification of biological molecules
ES2153807T3 (es) 1997-08-08 2005-06-16 Biomerieux B.V. Secuencias de acido nucleico que se pueden utilizar como cebadores y sondas en la amplificacion y deteccion de todos los subtipos de vih-1.
CA2304017C (en) 1997-09-17 2007-05-01 Gentra Systems, Inc. Apparatuses and methods for isolating nucleic acid
JP4304348B2 (ja) * 1997-09-22 2009-07-29 独立行政法人理化学研究所 Ｄｎａの単離方法
DE19746874A1 (de) * 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
JP4025399B2 (ja) * 1997-10-28 2007-12-19 株式会社日立製作所 核酸の回収方法及び装置
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
AU2509799A (en) * 1997-12-18 1999-07-12 Invitek Gmbh Method for isolating short and long-chain nucleic acids
ZA99493B (en) * 1998-01-30 1999-07-22 Akzo Nobel Nv Method for the isolation of nucleic acid.
AU2574499A (en) * 1998-02-02 1999-08-16 Gentra Systems, Inc. Compositions and methods for using a lysing matrix for isolating dna
JP4616990B2 (ja) * 1998-02-02 2011-01-19 キアジエン・ノース・アメリカン・ホールデイングス・インコーポレーテツド Ｄｎａを単離し、増幅し、そして特性化するための方法
JP2004500002A (ja) * 1998-02-02 2004-01-08 ジエントラ・システムズ・インコーポレーテツド Ｄｎａを単離するための溶出試薬、方法およびキット
EP2290099B1 (en) * 1998-02-02 2012-10-10 QIAGEN North American Holdings, Inc. Processes for isolating, amplifying and characterizing DNA
JPH11266864A (ja) * 1998-03-19 1999-10-05 Hitachi Ltd 核酸の精製方法および精製用装置
DE19818485B4 (de) * 1998-04-24 2005-03-24 IBL Gesellschaft für Immunchemie und Immunbiologie mbH Reaktionsgefäße, beschichtet mit einem cis-Diol-spezifischen Affinitätsmedium, sowie Verfahren zu deren Herstellung
DE19836559A1 (de) * 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
DE69935232T2 (de) * 1998-09-10 2008-01-10 Whatman Plc Produkt und Verfahren zum Auftrennen einer Probe, enthaltend genetisches Material aus mehreren Quellen, unter Verwendung eines festen Mediums
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
US6492162B1 (en) 1998-10-27 2002-12-10 Hitachi, Ltd. Apparatus for the recovery of nucleic acids
JP2000166556A (ja) * 1998-12-10 2000-06-20 Hitachi Ltd 核酸の回収方法及び装置
US7790865B1 (en) 1999-02-02 2010-09-07 Qiagen North American Holdings, Inc Eluting reagents, methods and kits for isolating DNA
CA2270106C (en) * 1999-04-23 2006-03-14 Yousef Haj-Ahmad Nucleic acid purification and process
EP1203240A2 (de) * 1999-08-09 2002-05-08 Bilatec Gesellschaft Zur Entwicklung Biotechnologischer Systeme MBH Labor-roboter und verfahren und reagenzienkit zur isolierung von nukleinsäuren
DE19943374A1 (de) 1999-09-10 2001-03-29 Max Planck Gesellschaft Verfahren zum Anbinden von Nukleinsäuren an eine Festphase
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
DE19946597A1 (de) * 1999-09-29 2001-04-12 Nigu Chemie Gmbh Stabilisiertes Guanidinlthiocyanat und dessen Verwendung
CA2392009C (en) 1999-11-17 2009-07-14 Roche Diagnostics Gmbh Magnetic glass particles, method for their preparation and uses thereof
GB0001450D0 (en) 2000-01-21 2000-03-08 Genpoint As Cell isolation method
DE10004927C2 (de) * 2000-02-04 2003-02-06 Qiagen Gmbh Nukleinsäure-Isolierung aus Stuhlproben und anderen biologischen Materialien, die reich an Inhibitoren sind
DE10006662A1 (de) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6815215B2 (en) 2000-02-28 2004-11-09 Hitachi, Ltd. Method of recovering a plurality of nucleic acids by an identical stationary phase and an apparatus thereof
US7183002B2 (en) 2000-03-24 2007-02-27 Qiagen, Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
GB0009771D0 (en) 2000-04-19 2000-06-07 Angiomed Ag Method for linking nucleic acids
GB0013658D0 (en) 2000-06-05 2000-07-26 Dynal Asa Nucleic acid isolation
WO2002078847A1 (en) * 2001-03-28 2002-10-10 Hitachi, Ltd. Instrument and method for recovering nucleic acid
US6818762B2 (en) 2001-05-25 2004-11-16 Maine Molecular Quality Controls, Inc. Compositions and methods relating to nucleic acid reference standards
JP2002191351A (ja) * 2001-10-19 2002-07-09 Hitachi Ltd 核酸の精製用装置および核酸捕捉用チップ
ATE449186T1 (de) 2001-11-28 2009-12-15 Applied Biosystems Llc Zusammensetzungen und verfahren zur selektiven nukleinsäureisolierung
EP2272988A3 (en) 2002-01-07 2011-06-15 Norchip A/S Method for detecting human papillomavirus mRNA
EP1466018B2 (en) 2002-01-08 2015-08-12 Roche Diagnostics GmbH Use of silica material in an amplification reaction
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
DE10231659B4 (de) * 2002-07-12 2006-01-19 Preanalytix Gmbh Zusammensetzung zum Binden von Nukleinsäure an eine Festphase
US9394332B2 (en) 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
WO2004079333A2 (en) * 2003-01-15 2004-09-16 Ic & G Co., Ltd. Method and kit for extracting rna from sputum
EP1590362B8 (en) 2003-01-29 2015-06-03 Epigenomics AG Improved method for bisulfite treatment
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
ES2335344T3 (es) * 2003-03-19 2010-03-25 Hamamatsu Foundation For Science And Technology Promotion Procedimiento para detectar un marcador de cancer de colon.
WO2005007852A2 (en) * 2003-07-09 2005-01-27 Genvault Corporation Room temperature elution of nucleic acids
WO2005008209A2 (ja) * 2003-07-16 2005-01-27 Toyo Boseki 生体成分分離用デバイス、およびそれを用いた生体成分の分離方法
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
WO2005054466A2 (en) † 2003-07-25 2005-06-16 Ambion, Inc. Methods and compositions for preparing rna from a fixed sample
GB0319671D0 (en) * 2003-08-21 2003-09-24 Secr Defence Apparatus for processing a fluid sample
WO2005054502A1 (en) 2003-12-02 2005-06-16 Roche Diagnostics Gmbh Improved method for bisulfite treatment
DE10358137A1 (de) * 2003-12-12 2005-07-07 Merck Patent Gmbh Verfahren und Kit zur Isolierung von RNA
US20050130177A1 (en) 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
AU2003300537A1 (en) * 2003-12-19 2005-07-21 Preanalytix Gmbh Composition for binding a nucleic acid to a solid phase
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
EP1719816B1 (en) 2004-02-12 2011-06-01 GL Sciences Incorporated Mechanism of separating and purifying dna
AU2005200670B2 (en) * 2004-02-20 2007-05-03 F. Hoffmann-La Roche Ag Adsorption of nucleic acids to a solid phase
JP4539125B2 (ja) * 2004-03-08 2010-09-08 ブラザー工業株式会社 核酸単離部材
US20050208501A1 (en) * 2004-03-16 2005-09-22 Ambion, Inc. Process and reagents for extraction of RNA from fractionated blood leukocytes
US7897378B2 (en) 2004-03-18 2011-03-01 Roche Molecular Systems, Inc. Method and device for purifying nucleic acids
JP4080462B2 (ja) * 2004-07-09 2008-04-23 株式会社日立製作所 核酸の回収方法
DE102004034433A1 (de) 2004-07-15 2006-02-02 Qiagen Gmbh Verfahren zur Reinigung und Isolierung von Nukleinsäuren unter Verwendung kationischer Detergentien
RU2272072C1 (ru) * 2004-08-26 2006-03-20 Павел Петрович Лактионов Способ выделения нуклеиновых кислот
EP1632578A1 (en) 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method
JP2006166711A (ja) * 2004-12-13 2006-06-29 Okayama Univ 糞便由来遺伝子の簡便増幅方法
JP4764966B2 (ja) * 2005-03-30 2011-09-07 独立行政法人科学技術振興機構 メッセンジャーｒｎａの分離方法
DE102005040259A1 (de) 2005-08-24 2007-03-01 Qiagen Gmbh Verfahren zur Gewinnung von Nukleinsäuren aus Blut
JP4699868B2 (ja) 2005-11-04 2011-06-15 株式会社日立ハイテクノロジーズ 核酸精製方法及び核酸精製器具
US7981606B2 (en) 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
JP5169826B2 (ja) 2006-06-20 2013-03-27 日立金属株式会社 金属微粒子及び生体物質抽出用の磁気ビーズ、並びにそれらの製造方法
JP2008005781A (ja) * 2006-06-29 2008-01-17 Olympus Corp 試料処理方法
US20080113357A1 (en) * 2006-06-29 2008-05-15 Millipore Corporation Filter device for the isolation of a nucleic acid
US10131935B2 (en) * 2006-07-11 2018-11-20 Aj Innuscreen Gmbh Method for parallel isolation of viral nucleic acids
EP1911844A1 (en) 2006-10-10 2008-04-16 Qiagen GmbH Methods and kit for isolating nucleic acids
CA2614069C (en) 2006-12-11 2016-05-03 F. Hoffmann-La Roche Ag Nucleic acid isolation using polidocanol and derivatives
EP1932913B1 (en) 2006-12-11 2013-01-16 Roche Diagnostics GmbH Nucleic acid isolation using polidocanol and derivatives
WO2008079905A1 (en) * 2006-12-21 2008-07-03 Invitrogen Dynal As Particles and their use in a method for isolating nucleic acid or a method for isolating phosphoproteins
DE102007009347B4 (de) 2007-02-27 2009-11-26 Agowa Gmbh Verfahren zur Isolierung von Nukleinsäuren
DE102007016707A1 (de) 2007-04-04 2008-10-09 Qiagen Gmbh Verfahren zur Aufreinigung von Biomolekülen
CA2629589C (en) * 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
CA2629586C (en) * 2007-04-20 2016-05-24 F.Hoffmann-La Roche Ag Adsorption of nucleic acids to solid phases under low-salt conditions
DE102007035250A1 (de) * 2007-07-27 2009-01-29 Qiagen Gmbh Verfahren zum Abtrennen von nicht-proteinhaltigen Biomolekülen, insbesondere Nukleinsäuren aus proteinhaltigen Proben
EP2036988A1 (en) 2007-09-12 2009-03-18 Siemens Healthcare Diagnostics GmbH A method for predicting the response of a tumor in a patient suffering from or at risk of developing recurrent gynecologic cancer towards a chemotherapeutic agent
JP4097687B2 (ja) * 2007-09-21 2008-06-11 株式会社日立製作所 核酸の回収方法
EP2065399A1 (en) 2007-11-30 2009-06-03 Siemens Healthcare Diagnostics GmbH The present invention relates to methods for prediction of the therapeutic success of breast cancer therapy
DE602008001350D1 (de) 2007-10-31 2010-07-08 Roche Diagnostics Gmbh Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren
EP2065474A1 (en) 2007-11-28 2009-06-03 Siemens Healthcare Diagnostics GmbH A method to assess prognosis and to predict therapeutic response to endocrine treatment
EP2065475A1 (en) 2007-11-30 2009-06-03 Siemens Healthcare Diagnostics GmbH Method for therapy prediction in tumors having irregularities in the expression of at least one VEGF ligand and/or at least one ErbB-receptor
US9932639B2 (en) 2007-11-30 2018-04-03 Ralph Markus Wirtz Method for predicting therapy responsiveness in basal like tumors
EP2071034A1 (en) 2007-12-12 2009-06-17 bioMérieux Method for treating a solution in order to destroy any ribonucleic acid after amplification
DE102008010692A1 (de) 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrices, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
DE102008010693A1 (de) 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrixmaterialien, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
EP2128169A1 (de) 2008-05-30 2009-12-02 Qiagen GmbH Verfahren zur Isolierung von kurzkettigen Nukleinsäuren
DE102008029356A1 (de) 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
US8158405B2 (en) 2008-06-30 2012-04-17 General Electric Company Process for concentrating and processing fluid samples
US8546127B2 (en) 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
ES2539856T3 (es) 2008-08-01 2015-07-06 F. Hoffmann-La Roche Ag Lisis mejorada y transcripción reversa para la cuantificación de mRNA
EP2157181A1 (de) 2008-08-13 2010-02-24 AGOWA Gesellschaft für molekularbiologische Technologie mbH Verfahren zur Isolation von Nukleinsäuren und Testkit
DE102008047790A1 (de) 2008-09-17 2010-04-15 Qiagen Gmbh Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe
CA2682439A1 (en) 2008-10-17 2010-04-17 F. Hoffmann-La Roche Ag Cell monitoring and molecular analysis
DE102008061714A1 (de) 2008-12-12 2010-06-17 Siemens Healthcare Diagnostics Inc., Deerfield Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe
FR2940805B1 (fr) 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
JP5570422B2 (ja) 2009-01-16 2014-08-13 アークレイ株式会社 核酸試料の製造方法、および、それを用いた核酸増幅物の製造方法
DE102009022513A1 (de) 2009-05-25 2010-12-02 Qiagen Gmbh Verfahren zum Schutz von Membranen
US20120271043A1 (en) * 2009-08-04 2012-10-25 John Steichen Process and device for collecting nucleic acids of microorganisms from a particulate sample
FR2959312B1 (fr) 2010-04-23 2023-01-20 Biomerieux Sa Dispositif de preparation et/ou de traitement d'un echantillon biologique
JP5454338B2 (ja) 2010-04-28 2014-03-26 株式会社島津製作所 液滴操作によるリアルタイム核酸増幅法
CN102276937B (zh) * 2010-06-12 2013-06-05 中国石油化工股份有限公司 一种抗菌聚苯乙烯组合物及其制备方法
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
US9725754B2 (en) 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
CN107058617A (zh) 2010-07-29 2017-08-18 霍夫曼-拉罗奇有限公司 通用样品制备
DE102010043015B4 (de) * 2010-10-27 2019-12-19 Robert Bosch Gmbh Verfahren zur Konzentration von Probenbestandteilen und Amplifikation von Nukleinsäuren
WO2012054975A1 (en) 2010-10-28 2012-05-03 Clinical Genomics Pty. Ltd. Method of microvesicle enrichment
JP5455878B2 (ja) 2010-11-30 2014-03-26 シスメックス株式会社 被検物質の電気化学的検出方法
US9517472B2 (en) 2010-12-21 2016-12-13 Shimadzu Corporation Device and method for processing target component in tube
KR101193765B1 (ko) * 2011-05-16 2012-10-24 (주)나노헬릭스 초고속 핵산의 정제방법
EP2546199A1 (en) 2011-07-14 2013-01-16 Université Catholique De Louvain Solid iron oxide support for isolation of microorganisms
JP5807542B2 (ja) 2011-12-22 2015-11-10 株式会社島津製作所 対象成分を操作するためのチップデバイス及びそれを用いた方法
CN104350152B (zh) * 2012-06-01 2017-08-11 欧米伽生物技术公司 选择性核酸片段回收
DE102012012523B4 (de) 2012-06-26 2015-02-12 Magnamedics Gmbh Reinigung von Nukleinsäuren
JP6024266B2 (ja) * 2012-08-01 2016-11-16 セイコーエプソン株式会社 Ｄｎａの抽出方法
JP6093530B2 (ja) * 2012-09-24 2017-03-08 東芝メディカルシステムズ株式会社 乳中の微生物を濃縮および乳中の微生物の核酸を回収する方法
JP6255034B2 (ja) 2012-12-11 2017-12-27 キアゲン ゲーエムベーハー シリカ粒子の調製
US10745686B2 (en) 2013-02-08 2020-08-18 Qiagen Gmbh Method for separating DNA by size
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
JP6431522B2 (ja) 2013-03-15 2018-11-28 アボツト・モレキユラー・インコーポレイテツド 核酸の精製のための１工程法
CN105358688B (zh) 2013-07-03 2019-01-18 株式会社岛津制作所 粒子操作方法以及粒子操作器件
EP3124608B1 (en) * 2014-03-26 2019-09-18 Universal Bio Research Co., Ltd. Method and reagent for extracting nucleic acid
EP4729528A3 (en) * 2014-04-25 2026-06-24 Translate Bio, Inc. Methods for purification of messenger rna
JP6681384B2 (ja) 2014-07-30 2020-04-15 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト 粒子を処理するための自動化システム
JP6437250B2 (ja) 2014-09-03 2018-12-12 株式会社ミズホメディー 前処理方法及びそれに用いられる核酸抽出キット
WO2016193490A1 (en) 2015-06-05 2016-12-08 Qiagen Gmbh Method for separating dna by size
ES2965460T3 (es) 2015-06-09 2024-04-15 Biocartis Nv Método automatizable para el aislamiento de ácidos nucleicos
EP3348634B1 (en) 2015-09-10 2023-08-09 Kaneka Corporation Method for separating nucleic acid from sample comprising nucleic acid, and device therefor
US11427815B2 (en) 2015-12-28 2022-08-30 Koninklijke Philips N.V. Nucleic acid purification system using a single wash and elution buffer solution
TWI694146B (zh) * 2016-02-02 2020-05-21 日商美之賀美蒂股份有限公司 前處理方法及用於其之核酸萃取套組
DK3205722T3 (en) * 2016-02-11 2018-11-26 Sarstedt Ag & Co Kg Device and methods for isolating nucleic acids from blood
SI3445850T1 (sl) * 2016-04-22 2021-12-31 BioNTech SE Postopki za zagotavljanje enoverižne RNA
JP6979797B2 (ja) * 2017-05-31 2021-12-15 シスメックス株式会社 固液分離方法、固液分離装置およびそれに用いるピペットチップ、粒子およびキット
CN107058297B (zh) * 2017-06-05 2018-01-16 陈礼平 一种离液剂以及使用该离液剂提取基因组dna的方法
CN108130325B (zh) * 2018-02-02 2024-06-18 苏州优卡新材料科技有限公司 一种核糖核酸提取用无机材料滤芯的高效纯化柱
JP2020024202A (ja) * 2018-08-06 2020-02-13 積水化学工業株式会社 分析用具及び洗浄方法
CA3120216A1 (en) 2018-11-19 2020-05-28 Biocartis Nv Enhanced detection of low-copy-number nucleic acids in an integrated workflow
US12565644B2 (en) 2019-08-08 2026-03-03 Biocartis Nv Nucleic acid purification chemistry
WO2022155246A1 (en) 2021-01-12 2022-07-21 Definitive Biotechnologies Llc Device and method for detecting nucleic acids in biological samples
NL2034698B1 (en) 2023-04-26 2024-11-04 Biocartis Nv Semi-solid phase extraction of nucleic acids using a quaternary salt and cation mixture in alkaline conditions

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59227744A (ja) * 1983-06-08 1984-12-21 Rikaken Kk Ｄｎａ回収用ガラス粉粒物
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA

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CA2271603A1 (en) 1990-09-23
GR3025351T3 (en) 1998-02-27
AU5215390A (en) 1990-09-27
KR900014594A (ko) 1990-10-24
DE69031237D1 (de) 1997-09-18
JPH1072485A (ja) 1998-03-17
DE389063T1 (de) 1996-10-10
DK0389063T3 (da) 1998-03-30
ZA902190B (en) 1991-12-24
EP0389063A3 (en) 1992-04-15
EP0819696A3 (en) 1998-07-15
NL8900725A (nl) 1990-10-16
EP0819696A2 (en) 1998-01-21
DE69031237T2 (de) 1998-01-02
JPH02289596A (ja) 1990-11-29
EP0389063A2 (en) 1990-09-26
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GR960300019T1 (en) 1996-03-31
DK0389063T4 (da) 2007-02-19
EP0389063B2 (en) 2006-10-25
JP2680462B2 (ja) 1997-11-19
ES2085245T1 (es) 1996-06-01
AU641641B2 (en) 1993-09-30
ATE156830T1 (de) 1997-08-15
ES2085245T5 (es) 2007-06-01
CA2012777C (en) 1999-08-31
EP0389063B1 (en) 1997-08-13
CA2012777A1 (en) 1990-09-23

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