ES2097768T3 - Amplificacion de la señal de una sonda de adn. - Google Patents

Amplificacion de la señal de una sonda de adn.

Info

Publication number
ES2097768T3
ES2097768T3 ES91105249T ES91105249T ES2097768T3 ES 2097768 T3 ES2097768 T3 ES 2097768T3 ES 91105249 T ES91105249 T ES 91105249T ES 91105249 T ES91105249 T ES 91105249T ES 2097768 T3 ES2097768 T3 ES 2097768T3
Authority
ES
Spain
Prior art keywords
molecule
developer
sequence
branch
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91105249T
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English (en)
Inventor
David Segev
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Individual
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2097768T3 publication Critical patent/ES2097768T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA INVENCION SE REFIERE A UN METODO PARA AMPLIFICAR UN SERIAL DURANTE LA DETECCION DE MOLECULAS DE ACIDO NUCLEICO DE BLANCO QUE CONSISTE EN LOS PASOS SIGUIENTES: A) HIBRIDIZAR UNA PRIMERA SECUENCIA DE UNA SONDA OLIGONUCLEOTIDA PRIMARIA A LAS MOLECULAS DE ACIDO NUCLEICO DE BLANCO EN DONDE LA SONDA PRIMARIA TIENE UN MEDIO PARA ENLAZARSE A UNA MOLECULA DE ACIDO NUCLEICO PUENTE, SIENDO LA MOLECULA PUENTE CAPAZ DE HIBRIDIZAR A POR LO MENOS UNA MOLECULA DE ACIDO NUCLEICO DESARROLLADORA; B) EXPONER LA SONDA PRIMARIA A LA MOLECULA PUENTE Y A UNA PRIMERA MOLECULA DESARROLLADORA Y A UNA SEGUNDA MOLECULA DESARROLLADORA, CONSTANDO CADA MOLECULA DESARROLLADORA DE: I) UNA PRIMERA RAMA QUE CONSTA DE UNA SECUENCIA DE POR LO MENOS DOS NUCLEOTIDOS DIFERENTES Y POR LO MENOS SEIS NUCLEOTIDOS TOTALES COMPLEMENTARIOS A UNA SECUENCIA DE UNA PRIMERA RAMA DE LA OTRA MOLECULA DESARROLLADORA; II) UNA SEGUNDA RAMA QUE CONSTA DE UNA SECUENCIA DE POR LO MENOS DOS NUCLEOTIDOS DIFERENTES Y POR LO MENOS SEIS NUCLEOTIDOS TOTALES COMPLEMENTARIOS A UNA SECUENCIA DE UNA SEGUNDA RAMA DE LA OTRA MOLECULA DESARROLLADORA; Y III) UNA ETIQUETA DETECTABLE O UN MEDIO DE SER CONVERTIDA EN UNA MOLECULA DESARROLLADORA QUE CONTIENE UNA ETIQUETA DETECTABLE; EN DONDE LA PRIMERA MOLECULA DESARROLLADORA Y LA SEGUNDA MOLECULA DESARROLLADORA TIENEN ESTRUCTURAS QUE SON IGUALES O DIFERENTES; Y LLEVAR A CABO LOS PASOS A Y B BAJO CONDICIONES TALES QUE: I) LA MOLECULA PUENTE SE ENLACE A LA SONDA PRIMARIA Y SE HIBRIDICE A LA PRIMERA MOLECULA DESARROLLADORA; Y II) LA PRIMERA MOLECULA DESARROLLADORA ENLAZADA SE HIBRIDICE A UNA SEGUNDA MOLECULA DESARROLLADORA PARA FORMAR UNA CADENA DESARROLLADORA; Y C) DETECTAR LA MOLECULA DESARROLLADORA ETIQUETADA EN LA CADENA DESARROLLADORA.
ES91105249T 1990-04-03 1991-04-03 Amplificacion de la señal de una sonda de adn. Expired - Lifetime ES2097768T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US50362190A 1990-04-03 1990-04-03

Publications (1)

Publication Number Publication Date
ES2097768T3 true ES2097768T3 (es) 1997-04-16

Family

ID=24002849

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91105249T Expired - Lifetime ES2097768T3 (es) 1990-04-03 1991-04-03 Amplificacion de la señal de una sonda de adn.

Country Status (7)

Country Link
US (1) US5437977A (es)
EP (1) EP0450594B1 (es)
JP (2) JP3142888B2 (es)
AT (1) ATE146530T1 (es)
CA (1) CA2039517C (es)
DE (1) DE69123621T2 (es)
ES (1) ES2097768T3 (es)

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Also Published As

Publication number Publication date
EP0450594B1 (en) 1996-12-18
JPH0788000A (ja) 1995-04-04
JP2000279186A (ja) 2000-10-10
DE69123621T2 (de) 1997-04-17
EP0450594A3 (en) 1992-01-22
US5437977A (en) 1995-08-01
CA2039517C (en) 2006-11-07
ATE146530T1 (de) 1997-01-15
JP3142888B2 (ja) 2001-03-07
DE69123621D1 (de) 1997-01-30
CA2039517A1 (en) 1991-10-04
EP0450594A2 (en) 1991-10-09

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