ES2097768T3 - Amplificacion de la señal de una sonda de adn. - Google Patents

Amplificacion de la señal de una sonda de adn.

Info

Publication number
ES2097768T3
ES2097768T3 ES91105249T ES91105249T ES2097768T3 ES 2097768 T3 ES2097768 T3 ES 2097768T3 ES 91105249 T ES91105249 T ES 91105249T ES 91105249 T ES91105249 T ES 91105249T ES 2097768 T3 ES2097768 T3 ES 2097768T3
Authority
ES
Spain
Prior art keywords
molecule
developer
sequence
branch
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91105249T
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English (en)
Inventor
David Segev
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Individual
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2097768T3 publication Critical patent/ES2097768T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA INVENCION SE REFIERE A UN METODO PARA AMPLIFICAR UN SERIAL DURANTE LA DETECCION DE MOLECULAS DE ACIDO NUCLEICO DE BLANCO QUE CONSISTE EN LOS PASOS SIGUIENTES: A) HIBRIDIZAR UNA PRIMERA SECUENCIA DE UNA SONDA OLIGONUCLEOTIDA PRIMARIA A LAS MOLECULAS DE ACIDO NUCLEICO DE BLANCO EN DONDE LA SONDA PRIMARIA TIENE UN MEDIO PARA ENLAZARSE A UNA MOLECULA DE ACIDO NUCLEICO PUENTE, SIENDO LA MOLECULA PUENTE CAPAZ DE HIBRIDIZAR A POR LO MENOS UNA MOLECULA DE ACIDO NUCLEICO DESARROLLADORA; B) EXPONER LA SONDA PRIMARIA A LA MOLECULA PUENTE Y A UNA PRIMERA MOLECULA DESARROLLADORA Y A UNA SEGUNDA MOLECULA DESARROLLADORA, CONSTANDO CADA MOLECULA DESARROLLADORA DE: I) UNA PRIMERA RAMA QUE CONSTA DE UNA SECUENCIA DE POR LO MENOS DOS NUCLEOTIDOS DIFERENTES Y POR LO MENOS SEIS NUCLEOTIDOS TOTALES COMPLEMENTARIOS A UNA SECUENCIA DE UNA PRIMERA RAMA DE LA OTRA MOLECULA DESARROLLADORA; II) UNA SEGUNDA RAMA QUE CONSTA DE UNA SECUENCIA DE POR LO MENOS DOS NUCLEOTIDOS DIFERENTES Y POR LO MENOS SEIS NUCLEOTIDOS TOTALES COMPLEMENTARIOS A UNA SECUENCIA DE UNA SEGUNDA RAMA DE LA OTRA MOLECULA DESARROLLADORA; Y III) UNA ETIQUETA DETECTABLE O UN MEDIO DE SER CONVERTIDA EN UNA MOLECULA DESARROLLADORA QUE CONTIENE UNA ETIQUETA DETECTABLE; EN DONDE LA PRIMERA MOLECULA DESARROLLADORA Y LA SEGUNDA MOLECULA DESARROLLADORA TIENEN ESTRUCTURAS QUE SON IGUALES O DIFERENTES; Y LLEVAR A CABO LOS PASOS A Y B BAJO CONDICIONES TALES QUE: I) LA MOLECULA PUENTE SE ENLACE A LA SONDA PRIMARIA Y SE HIBRIDICE A LA PRIMERA MOLECULA DESARROLLADORA; Y II) LA PRIMERA MOLECULA DESARROLLADORA ENLAZADA SE HIBRIDICE A UNA SEGUNDA MOLECULA DESARROLLADORA PARA FORMAR UNA CADENA DESARROLLADORA; Y C) DETECTAR LA MOLECULA DESARROLLADORA ETIQUETADA EN LA CADENA DESARROLLADORA.
ES91105249T 1990-04-03 1991-04-03 Amplificacion de la señal de una sonda de adn. Expired - Lifetime ES2097768T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US50362190A 1990-04-03 1990-04-03

Publications (1)

Publication Number Publication Date
ES2097768T3 true ES2097768T3 (es) 1997-04-16

Family

ID=24002849

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91105249T Expired - Lifetime ES2097768T3 (es) 1990-04-03 1991-04-03 Amplificacion de la señal de una sonda de adn.

Country Status (7)

Country Link
US (1) US5437977A (es)
EP (1) EP0450594B1 (es)
JP (2) JP3142888B2 (es)
AT (1) ATE146530T1 (es)
CA (1) CA2039517C (es)
DE (1) DE69123621T2 (es)
ES (1) ES2097768T3 (es)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2663292A (en) * 1991-09-10 1993-04-05 Jack D. Love Dna/rna target and signal amplification
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US6180338B1 (en) 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
US6207368B1 (en) 1992-08-04 2001-03-27 Beckman Coulter, Inc. Methods and reagents for controlling chain extension and ligation chain reactions
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
AU692212B2 (en) * 1993-12-17 1998-06-04 Roger S. Cubicciotti Nucleotide-directed assembly of bimolecular and multimolecular drugs and devices
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US6986985B1 (en) 1994-01-13 2006-01-17 Enzo Life Sciences, Inc. Process for producing multiple nucleic acid copies in vivo using a protein-nucleic acid construct
US7569341B2 (en) * 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
US5561043A (en) * 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
CA2126952C (en) * 1994-06-28 2010-12-14 Sithian Pandian Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays
US6306657B1 (en) * 1994-06-28 2001-10-23 Kalyx Biosciences, Inc. Polynucleotide probe and kit for amplifying signal detection in hybridization assays
US20030104361A1 (en) 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
US5871902A (en) * 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US5843650A (en) * 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
DE19537952A1 (de) * 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
JP2002515730A (ja) * 1995-10-16 2002-05-28 カイロン コーポレイション 遺伝子発現を変調する因子のスクリーニング方法
CA2259918A1 (en) 1996-07-12 1998-01-22 Tm Technologies, Inc. Signal amplification method
US5955590A (en) * 1996-07-15 1999-09-21 Worcester Foundation For Biomedical Research Conjugates of minor groove DNA binders with antisense oligonucleotides
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
CA2218440A1 (en) * 1996-12-21 1998-06-21 Boehringer Mannheim Gmbh Method of analysis using signal amplification
US6187536B1 (en) 1997-02-18 2001-02-13 Thomas Jefferson University Methods of identifying and detecting pancreatic cancer
JP4221145B2 (ja) * 1997-07-29 2009-02-12 ポリプローブ，インコーポレイテッド 最大の自己アセンブリを行う樹枝状核酸
US6120995A (en) * 1997-08-07 2000-09-19 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
US7135333B1 (en) 1997-08-07 2006-11-14 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
WO1999024612A2 (en) * 1997-11-06 1999-05-20 Mosaic Technologies Multiple sequential polynucleotide displacement reactions for signal amplification and processing
PT1038022E (pt) * 1997-12-12 2005-11-30 Digene Corp Avaliacao de doencas relacionadas com o virus papiloma humano
US20100105572A1 (en) * 1997-12-19 2010-04-29 Kris Richard M High throughput assay system
US6238927B1 (en) 1998-10-05 2001-05-29 Mosaic Technologies, Incorporated Reverse displacement assay for detection of nucleic acid sequences
JP3267576B2 (ja) * 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
US6432642B1 (en) 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
WO2000075303A1 (en) * 1999-06-07 2000-12-14 Kenshi Obaru Primers for polymerase reactions
ATE452989T1 (de) 2000-03-27 2010-01-15 Univ Jefferson Zusammensetzungen und methoden zur identifizierung von krebszellen
JP3310662B2 (ja) * 2000-03-31 2002-08-05 三光純薬株式会社 プローブポリマー作製用プローブ、プローブポリマーの作製方法及びその利用
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7829276B2 (en) * 2000-09-18 2010-11-09 Thomas Jefferson University Methods of using CRCA-1 as a stomach and esophageal cancer marker
JP3912595B2 (ja) 2000-10-11 2007-05-09 三光純薬株式会社 プローブ自己集合体の作製方法
EP1401850A1 (en) 2001-06-20 2004-03-31 Nuevolution A/S Nucleoside derivatives for library preparation
US8512953B2 (en) 2001-09-24 2013-08-20 One Lambda Diagnostic probe detection system
CN1464910A (zh) * 2001-09-28 2003-12-31 三光纯药株式会社 Dna芯片的信号扩增方法
US20040029142A1 (en) * 2002-02-11 2004-02-12 Schon Eric A. Concatenation-based nucleic acid detection compositions and methods
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
AU2003247266A1 (en) 2002-08-01 2004-02-23 Nuevolution A/S Multi-step synthesis of templated molecules
PT1558744E (pt) * 2002-10-30 2011-09-22 Nuevolution As Codificação enzimática
WO2004056994A2 (en) * 2002-12-19 2004-07-08 Nuevolution A/S Quasirandom structure and function guided synthesis methods
EP1597395A2 (en) 2003-02-21 2005-11-23 Nuevolution A/S Method for producing second-generation library
BRPI0408967B8 (pt) 2003-03-31 2021-07-27 Hoffmann La Roche kit e métodos para detecção de um ácido nucléico de vários membros do sorogrupo do vírus da encefalite japonesa em uma amostra biológica sob condições de hibridização rigorosas
US7514551B2 (en) 2003-04-03 2009-04-07 Enzo Life Sciences, Inc. Multisignal labeling reagents, and processes and uses therefor
US9696298B2 (en) 2003-04-03 2017-07-04 Enzo Life Sciences, Inc. Multisignal reagents for labeling analytes
US9156986B2 (en) 2003-04-03 2015-10-13 Enzo Life Sciences, Inc. Multisignal labeling reagents and processes and uses therefor
DE602004023960D1 (de) 2003-09-18 2009-12-17 Nuevolution As Methode zur Gewinnung struktureller Informationen kodierter Moleküle und zur Selektion von Verbindungen
US7306915B2 (en) * 2004-03-22 2007-12-11 Sysmex Corporation Probe set for detection of target substance and detection method using the same
DE602005023002D1 (de) * 2004-04-28 2010-09-30 Eisai R&D Man Co Ltd Hybridisierungsverfahren
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
JPWO2006028162A1 (ja) * 2004-09-08 2008-05-08 エーザイ・アール・アンド・ディー・マネジメント株式会社 シグナルプローブポリマーの形成方法
US7842794B2 (en) 2004-12-17 2010-11-30 Roche Molecular Systems, Inc. Reagents and methods for detecting Neisseria gonorrhoeae
US20070048759A1 (en) * 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
US9574189B2 (en) * 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries
US8524453B2 (en) * 2006-02-10 2013-09-03 The Brigham And Woman's Hospital, Inc. Lectin complement pathway assays and related compositions and methods
US20070275388A1 (en) * 2006-05-25 2007-11-29 Daniel Ryan Dendrimers that possess a single target annealing site and uses thereof
EP2056709A4 (en) 2006-08-11 2013-05-01 Univ New Jersey Med DOUBLE-SENSITIZER WITH LUMINESCENCE COMPOUNDS, CONJUGATES AND USE
US8450058B2 (en) * 2007-08-14 2013-05-28 Eisai R&D Management Co., Ltd. Method of detecting target substance
SG185315A1 (en) * 2007-10-24 2012-11-29 Eisai R&D Man Co Ltd Nucleic acid probe, and method of forming probe -polymer
KR20100042082A (ko) * 2008-10-15 2010-04-23 삼성전자주식회사 신호물질의 밀도가 증가되어 있는 고체 지지체, 그를 포함하는 키트 및 그를 이용한 표적물질 검출 방법
JP2012506705A (ja) 2008-10-27 2012-03-22 キアジェン ゲイサーズバーグ インコーポレイテッド 迅速に結果を得るハイブリッドキャプチャー法による分析およびシステム
CA2750338C (en) * 2009-01-28 2019-06-25 Qiagen Gaithersburg, Inc. Sequence-specific large volume sample preparation method and assay
CN102414327B (zh) * 2009-05-01 2015-04-08 奇亚根盖瑟斯堡股份有限公司 用于在样品中检测rna剪接形式的非靶向扩增方法
US20100316998A1 (en) * 2009-06-15 2010-12-16 Massachusetts Institute Of Technology Methods and compositions for empirical selection of drugs or other molecules
WO2011032124A1 (en) 2009-09-14 2011-03-17 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
WO2011088193A2 (en) 2010-01-13 2011-07-21 University Of Medicine And Dentistry Of New Jersey Fluorophore chelated lanthanide luminiscent probes with improved quantum efficiency
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
JP6103941B2 (ja) 2010-01-29 2017-03-29 キアジェン ゲイサーズバーグ インコーポレイテッド 核酸の配列特異的精製および多重分析のための方法および組成物
CA2799200A1 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
WO2012027623A2 (en) 2010-08-25 2012-03-01 Pacific Biosciences Of California, Inc. Cyanine dyes
WO2012116220A2 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
US10385392B2 (en) 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes
WO2014145620A2 (en) * 2013-03-15 2014-09-18 The Broad Institute, Inc. Novel hybridization probes and uses thereof
JP2016538882A (ja) 2013-10-30 2016-12-15 グリーン ライフ バイオテック，エルエルシー カンキツグリーニング病のための病因定量システム及び治療方法
JP7093355B2 (ja) * 2016-12-16 2022-06-29 アラトム， エルエルシー ライゲーション増幅を使用する分子検出
EP3995590A1 (en) * 2017-10-11 2022-05-11 MGI Tech Co., Ltd. Method for improving loading and stability of nucleic acid
CN114544937B (zh) * 2020-11-11 2023-04-07 艾克发(北京)生物技术有限公司 一种多重信号放大系统及其在免疫吸附间接法检测中的应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4762780A (en) * 1984-04-17 1988-08-09 The Regents Of The University Of California Method and composition for screening and diagnosing "HCMV"
US4882669A (en) * 1983-11-28 1989-11-21 Canon Kabushiki Kaisha Multi computer fail safe control apparatus
US4724202A (en) * 1983-12-12 1988-02-09 Molecular Diagnostics, Inc. Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization
FI71768C (fi) * 1984-02-17 1987-02-09 Orion Yhtymae Oy Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning.
GB8405437D0 (en) * 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
DE3420925A1 (de) * 1984-06-05 1985-12-05 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zum nachweis einer nukleinsaeure-sequenz
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DE3680156D1 (de) * 1985-05-31 1991-08-14 Amoco Corp Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen.
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
EP0245206A1 (en) * 1986-05-05 1987-11-11 IntraCel Corporation Analytical method for detecting and measuring specifically sequenced nucleic acid
IL86164A0 (en) * 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
CA1339351C (en) * 1987-10-15 1997-08-26 Michael S. Urdea Nucleic acid multimers and amplified nucleic acid hybridization assays using same

Also Published As

Publication number Publication date
EP0450594B1 (en) 1996-12-18
JP3142888B2 (ja) 2001-03-07
JPH0788000A (ja) 1995-04-04
EP0450594A3 (en) 1992-01-22
DE69123621T2 (de) 1997-04-17
CA2039517A1 (en) 1991-10-04
US5437977A (en) 1995-08-01
CA2039517C (en) 2006-11-07
ATE146530T1 (de) 1997-01-15
DE69123621D1 (de) 1997-01-30
JP2000279186A (ja) 2000-10-10
EP0450594A2 (en) 1991-10-09

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