ATE146530T1 - Signalamplifikation einer dns-sonde - Google Patents

Signalamplifikation einer dns-sonde

Info

Publication number
ATE146530T1
ATE146530T1 AT91105249T AT91105249T ATE146530T1 AT E146530 T1 ATE146530 T1 AT E146530T1 AT 91105249 T AT91105249 T AT 91105249T AT 91105249 T AT91105249 T AT 91105249T AT E146530 T1 ATE146530 T1 AT E146530T1
Authority
AT
Austria
Prior art keywords
molecule
developer
sequence
branch
nucleic acid
Prior art date
Application number
AT91105249T
Other languages
English (en)
Inventor
David Segev
Original Assignee
David Segev
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by David Segev filed Critical David Segev
Application granted granted Critical
Publication of ATE146530T1 publication Critical patent/ATE146530T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT91105249T 1990-04-03 1991-04-03 Signalamplifikation einer dns-sonde ATE146530T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US50362190A 1990-04-03 1990-04-03

Publications (1)

Publication Number Publication Date
ATE146530T1 true ATE146530T1 (de) 1997-01-15

Family

ID=24002849

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91105249T ATE146530T1 (de) 1990-04-03 1991-04-03 Signalamplifikation einer dns-sonde

Country Status (7)

Country Link
US (1) US5437977A (de)
EP (1) EP0450594B1 (de)
JP (2) JP3142888B2 (de)
AT (1) ATE146530T1 (de)
CA (1) CA2039517C (de)
DE (1) DE69123621T2 (de)
ES (1) ES2097768T3 (de)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0648281A4 (de) * 1991-09-10 1997-06-04 Jack D Love Zielgerichte amplifikation von dna und rna und signalamplifikation.
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US6180338B1 (en) 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
US6207368B1 (en) 1992-08-04 2001-03-27 Beckman Coulter, Inc. Methods and reagents for controlling chain extension and ligation chain reactions
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
WO1995016788A1 (en) * 1993-12-17 1995-06-22 Cubicciotti Roger S Nucleotide-directed assembly of bimolecular and multimolecular drugs and devices
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US6986985B1 (en) 1994-01-13 2006-01-17 Enzo Life Sciences, Inc. Process for producing multiple nucleic acid copies in vivo using a protein-nucleic acid construct
US5561043A (en) * 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
US7569341B2 (en) * 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
CA2126952C (en) * 1994-06-28 2010-12-14 Sithian Pandian Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays
US6306657B1 (en) * 1994-06-28 2001-10-23 Kalyx Biosciences, Inc. Polynucleotide probe and kit for amplifying signal detection in hybridization assays
US5871902A (en) * 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US20030104361A1 (en) 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
US5843650A (en) * 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
DE19537952A1 (de) * 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
WO1997014812A2 (en) * 1995-10-16 1997-04-24 Chiron Corporation Method of screening for factors that modulate gene expression
AU4040097A (en) * 1996-07-12 1998-02-09 Tm Technologies, Inc. Signal amplification method
US5955590A (en) * 1996-07-15 1999-09-21 Worcester Foundation For Biomedical Research Conjugates of minor groove DNA binders with antisense oligonucleotides
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
CA2218440A1 (en) * 1996-12-21 1998-06-21 Boehringer Mannheim Gmbh Method of analysis using signal amplification
US6187536B1 (en) 1997-02-18 2001-02-13 Thomas Jefferson University Methods of identifying and detecting pancreatic cancer
EP1005573B1 (de) * 1997-07-29 2006-05-24 Polyprobe, Inc. Dendritische nukleinsäuren mit maximaler selbstzusammenlagerung
US7135333B1 (en) 1997-08-07 2006-11-14 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
US6120995A (en) * 1997-08-07 2000-09-19 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
US6255051B1 (en) 1997-11-06 2001-07-03 Mosaic Technologies Inc. Multiple sequential polynucleotide displacement reactions for signal amplification and processing
BR9814271A (pt) * 1997-12-12 2001-03-20 Digene Corp Determinação de doenças relacionadas com o vìrus do papiloma humano
US20100105572A1 (en) * 1997-12-19 2010-04-29 Kris Richard M High throughput assay system
US6238927B1 (en) 1998-10-05 2001-05-29 Mosaic Technologies, Incorporated Reverse displacement assay for detection of nucleic acid sequences
JP3267576B2 (ja) * 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
US6432642B1 (en) * 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
AU5103300A (en) * 1999-06-07 2000-12-28 Kenshi Obaru Primers for polymerase reactions
US20010039016A1 (en) * 2000-03-27 2001-11-08 Waldman Scott A. Use of expression profiling for identifying molecular markers useful for diagnosis of metastatic cancer
ES2317893T3 (es) * 2000-03-31 2009-05-01 Sanko Junyaku Co., Ltd. Sondas para construir polimeros de sondas, procedimiento para construir un polimero de sondas y uso del mismo.
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
AU2001292728A1 (en) * 2000-09-18 2002-03-26 Thomas Jefferson University Compositions and methods for identifying and targeting stomach and esophageal cancer cells
WO2002031192A1 (en) * 2000-10-11 2002-04-18 Sanko Junyaku Co., Ltd. Method of constructing self-assembly of probes and method of detecting the same
EP1401850A1 (de) 2001-06-20 2004-03-31 Nuevolution A/S Nukleosidderivate zur herstellung einer verbindungsbibliothek
EP2980222A1 (de) * 2001-09-24 2016-02-03 One Lambda, Inc. Diagnosesondendetektionssystem
JPWO2003029441A1 (ja) * 2001-09-28 2005-01-13 三光純薬株式会社 Ｄｎａチップのシグナル増幅方法
US20040029142A1 (en) * 2002-02-11 2004-02-12 Schon Eric A. Concatenation-based nucleic acid detection compositions and methods
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
WO2004013070A2 (en) 2002-08-01 2004-02-12 Nuevolution A/S Multi-step synthesis of templated molecules
DK3299463T3 (da) * 2002-10-30 2020-12-07 Nuevolution As Enzymatisk kodning
ATE450609T1 (de) * 2002-12-19 2009-12-15 Nuevolution As Durch quasizufallsstrukturen und funktionen geführte synthesemethode
US20070026397A1 (en) 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
KR20070121853A (ko) 2003-03-31 2007-12-27 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
US7514551B2 (en) 2003-04-03 2009-04-07 Enzo Life Sciences, Inc. Multisignal labeling reagents, and processes and uses therefor
US9156986B2 (en) 2003-04-03 2015-10-13 Enzo Life Sciences, Inc. Multisignal labeling reagents and processes and uses therefor
US9696298B2 (en) 2003-04-03 2017-07-04 Enzo Life Sciences, Inc. Multisignal reagents for labeling analytes
WO2005026387A1 (en) 2003-09-18 2005-03-24 Nuevolution A/S A method for obtaining structural information concerning an encoded molecule and method for selecting compounds
US7306915B2 (en) * 2004-03-22 2007-12-11 Sysmex Corporation Probe set for detection of target substance and detection method using the same
DE602005023002D1 (de) * 2004-04-28 2010-09-30 Eisai R&D Man Co Ltd Hybridisierungsverfahren
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
DE602005020319D1 (de) * 2004-09-08 2010-05-12 Eisai R&D Man Co Ltd Verfahren zur bildung eines signalsondenpolymers
US7842794B2 (en) 2004-12-17 2010-11-30 Roche Molecular Systems, Inc. Reagents and methods for detecting Neisseria gonorrhoeae
US20070048759A1 (en) * 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
LT2336315T (lt) * 2005-12-01 2017-11-10 Nuevolution A/S Fermentiniai kodavimo būdai, skirti efektyviai didelių bibliotekų sintezei
US8524453B2 (en) * 2006-02-10 2013-09-03 The Brigham And Woman's Hospital, Inc. Lectin complement pathway assays and related compositions and methods
US20070275388A1 (en) * 2006-05-25 2007-11-29 Daniel Ryan Dendrimers that possess a single target annealing site and uses thereof
US9415111B2 (en) 2006-08-11 2016-08-16 Rutgers, The State University Of New Jersey Dual-sensitizer-containing luminescent compounds, conjugates, and uses thereof
JP5289314B2 (ja) * 2007-08-14 2013-09-11 エーザイ・アール・アンド・ディー・マネジメント株式会社 標的物質の検出方法
SG185315A1 (en) * 2007-10-24 2012-11-29 Eisai R&D Man Co Ltd Nucleic acid probe, and method of forming probe -polymer
KR20100042082A (ko) * 2008-10-15 2010-04-23 삼성전자주식회사 신호물질의 밀도가 증가되어 있는 고체 지지체, 그를 포함하는 키트 및 그를 이용한 표적물질 검출 방법
TWI417389B (zh) * 2008-10-27 2013-12-01 Qiagen Gaithersburg Inc 於自動平台上之快速結果雜交捕捉法
EP2385979B1 (de) * 2009-01-28 2017-08-16 QIAGEN Gaithersburg, Inc. Sequenzspezifisches verfahren zur vorbereitung grossvolumiger proben und assay
AU2010242867B2 (en) * 2009-05-01 2016-05-12 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of RNA splice-forms in a sample
US20100316998A1 (en) * 2009-06-15 2010-12-16 Massachusetts Institute Of Technology Methods and compositions for empirical selection of drugs or other molecules
JP5826752B2 (ja) 2009-09-14 2015-12-02 キアジェン ゲイサーズバーグ インコーポレイテッド 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法
WO2011088193A2 (en) 2010-01-13 2011-07-21 University Of Medicine And Dentistry Of New Jersey Fluorophore chelated lanthanide luminiscent probes with improved quantum efficiency
ES2615728T3 (es) 2010-01-29 2017-06-08 Qiagen Gaithersburg, Inc. Métodos y composiciones para una purificación y un análisis múltiple específico de secuencia de ácidos nucleicos
CA2787781A1 (en) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of an hpv in a sample
US9422593B2 (en) 2010-05-19 2016-08-23 Qiagen Gaithresburg, Inc Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
CA2799205A1 (en) 2010-05-25 2011-12-01 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
US8889886B2 (en) * 2010-08-25 2014-11-18 Pacific Biosciences, Inc. Cyanine dyes
AU2012220575B2 (en) 2011-02-24 2017-01-12 Qiagen Gaithersburg, Inc. Materials and methods for detection of HPV nucleic acid
US10385392B2 (en) 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes
WO2014145620A2 (en) * 2013-03-15 2014-09-18 The Broad Institute, Inc. Novel hybridization probes and uses thereof
US10351918B2 (en) 2013-10-30 2019-07-16 Green Life Biotech, Llc Pathogenesis quantification systems and treatment methods for citrus greening blight
CN110300806B (zh) * 2016-12-16 2024-03-19 艾瑞特姆有限责任公司 使用连接扩增的分子检测
CN111148847B (zh) * 2017-10-11 2024-05-07 深圳华大智造科技有限公司 用于改善核酸装载和稳定性的方法
CN114544937B (zh) * 2020-11-11 2023-04-07 艾克发(北京)生物技术有限公司 一种多重信号放大系统及其在免疫吸附间接法检测中的应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4762780A (en) * 1984-04-17 1988-08-09 The Regents Of The University Of California Method and composition for screening and diagnosing "HCMV"
US4882669A (en) * 1983-11-28 1989-11-21 Canon Kabushiki Kaisha Multi computer fail safe control apparatus
US4724202A (en) * 1983-12-12 1988-02-09 Molecular Diagnostics, Inc. Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization
FI71768C (fi) * 1984-02-17 1987-02-09 Orion Yhtymae Oy Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning.
GB8405437D0 (en) * 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
DE3420925A1 (de) 1984-06-05 1985-12-05 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zum nachweis einer nukleinsaeure-sequenz
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
ATE65092T1 (de) * 1985-05-31 1991-07-15 Amoco Corp Verstaerkung von hybridisierungssignalen durch verwendung von komplementarischen dns-straengen.
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
EP0245206A1 (de) * 1986-05-05 1987-11-11 IntraCel Corporation Analytisches Verfahren zum Nachweis und zur Messung einer spezifisch sequentierten Nukleinsäure
IL86164A0 (en) * 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
CA1339351C (en) * 1987-10-15 1997-08-26 Michael S. Urdea Nucleic acid multimers and amplified nucleic acid hybridization assays using same

Also Published As

Publication number Publication date
EP0450594A2 (de) 1991-10-09
EP0450594B1 (de) 1996-12-18
EP0450594A3 (en) 1992-01-22
US5437977A (en) 1995-08-01
ES2097768T3 (es) 1997-04-16
DE69123621T2 (de) 1997-04-17
JP3142888B2 (ja) 2001-03-07
DE69123621D1 (de) 1997-01-30
JPH0788000A (ja) 1995-04-04
CA2039517C (en) 2006-11-07
CA2039517A1 (en) 1991-10-04
JP2000279186A (ja) 2000-10-10

Similar Documents

Publication Publication Date Title
DE69123621D1 (de) Signalamplifikation einer DNS-Sonde
DE69233630T2 (de) Sätze von HIV-Sonden zur Verwendung in Sandwich-Hydridisierungsassays in Lösungsphase
KR920701480A (ko) Hbv에 대한 개량된 증폭 핵산혼성화분석법
DE69322266D1 (de) Proben zusammensetzung und verfahren
ATE188744T1 (de) Verfahren zur erhöhung der nachweisempfindlichkeit von nukleinsäure-hybriden (target/probe)
CA2217325A1 (en) Nucleic acid detection and amplification by chemical linkage of oligonucleotides
ES2147180T3 (es) Procedimiento para la deteccion de acidos nucleicos semejantes.
ATE236269T1 (de) Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
ATE339514T1 (de) Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm)
DE69603723D1 (de) Verfahren zum nachweis von nukleinsäuren
ATE262593T1 (de) Chemisches verfahren zur vervielfältigung und detektion von nukleinsäuresequenzen
AU6275099A (en) Methods and compositions for amplifying detectable signals in specific binding assays
DE60233477D1 (de) Mobilitätsmodifizierte nukleobasenpolymere und verfahren zu deren verwendung
FI950487A7 (fi) Menetelmä, reagenssi ja testipakkaus nukleiinihapposekvenssien totamis eksi ja monistamiseksi
ATE250142T1 (de) Methode zur sequenzierung von polynukleotiden
DK0805876T3 (da) Nukleinsyreamplifikationsoligonukleotider og prober til Lyme-sygdom-associeret Borrelia
DE69937447D1 (de) Verfahren zur erkennung von nukleinsäuren
CA2129444A1 (en) Amplification of assay reporters by nucleic acid replication
SE9703251L (sv) Förfarande att välja sekvens hos sond för nukleinsyrehybridisering
Beck Colorimetric-detected DNA sequencing
DK0523557T3 (da) Immobilisering af nukleinsyrer
DE69709804D1 (de) Nachweis von nukleotidsequenzen durch signalamplifizierung
KR0169057B1 (ko) 핵산의 검출법
FI920636A7 (fi) DNA-koettimia ja alukkeita B. burgdorferin havaitsemiseksi polymeraasi ketjureaktiota käyttäen
US5824474A (en) Method for detecting nucleic acid

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time