ATE146530T1 - Signalamplifikation einer dns-sonde - Google Patents

Signalamplifikation einer dns-sonde

Info

Publication number
ATE146530T1
ATE146530T1 AT91105249T AT91105249T ATE146530T1 AT E146530 T1 ATE146530 T1 AT E146530T1 AT 91105249 T AT91105249 T AT 91105249T AT 91105249 T AT91105249 T AT 91105249T AT E146530 T1 ATE146530 T1 AT E146530T1
Authority
AT
Austria
Prior art keywords
molecule
developer
sequence
branch
nucleic acid
Prior art date
Application number
AT91105249T
Other languages
English (en)
Inventor
David Segev
Original Assignee
David Segev
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by David Segev filed Critical David Segev
Application granted granted Critical
Publication of ATE146530T1 publication Critical patent/ATE146530T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT91105249T 1990-04-03 1991-04-03 Signalamplifikation einer dns-sonde ATE146530T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US50362190A 1990-04-03 1990-04-03

Publications (1)

Publication Number Publication Date
ATE146530T1 true ATE146530T1 (de) 1997-01-15

Family

ID=24002849

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91105249T ATE146530T1 (de) 1990-04-03 1991-04-03 Signalamplifikation einer dns-sonde

Country Status (7)

Country Link
US (1) US5437977A (de)
EP (1) EP0450594B1 (de)
JP (2) JP3142888B2 (de)
AT (1) ATE146530T1 (de)
CA (1) CA2039517C (de)
DE (1) DE69123621T2 (de)
ES (1) ES2097768T3 (de)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993005184A1 (en) * 1991-09-10 1993-03-18 Love Jack D Dna/rna target and signal amplification
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US6207368B1 (en) 1992-08-04 2001-03-27 Beckman Coulter, Inc. Methods and reagents for controlling chain extension and ligation chain reactions
US6180338B1 (en) 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
AU692212B2 (en) * 1993-12-17 1998-06-04 Roger S. Cubicciotti Nucleotide-directed assembly of bimolecular and multimolecular drugs and devices
US6986985B1 (en) 1994-01-13 2006-01-17 Enzo Life Sciences, Inc. Process for producing multiple nucleic acid copies in vivo using a protein-nucleic acid construct
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US7569341B2 (en) * 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
US5561043A (en) * 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
CA2126952C (en) * 1994-06-28 2010-12-14 Sithian Pandian Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays
US6306657B1 (en) * 1994-06-28 2001-10-23 Kalyx Biosciences, Inc. Polynucleotide probe and kit for amplifying signal detection in hybridization assays
US5871902A (en) * 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US20030104361A1 (en) 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
US5843650A (en) * 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
DE19537952A1 (de) 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
JP2002515730A (ja) * 1995-10-16 2002-05-28 カイロン コーポレイション 遺伝子発現を変調する因子のスクリーニング方法
EP0938588B1 (de) * 1996-07-12 2003-02-12 TM Technologies Inc. Verfahren zur signalverstärkung
US5955590A (en) * 1996-07-15 1999-09-21 Worcester Foundation For Biomedical Research Conjugates of minor groove DNA binders with antisense oligonucleotides
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
CA2218440A1 (en) * 1996-12-21 1998-06-21 Boehringer Mannheim Gmbh Method of analysis using signal amplification
AU6659298A (en) 1997-02-18 1998-09-08 Thomas Jefferson University Compositions that bind to pancreatic cancer cells and methods of using the same
WO1999006595A1 (en) * 1997-07-29 1999-02-11 Polyprobe, Inc. Dendritic nucleic acids exhibiting maximal self-assembly
US6120995A (en) * 1997-08-07 2000-09-19 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
US7135333B1 (en) 1997-08-07 2006-11-14 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
AU1384299A (en) * 1997-11-06 1999-05-31 Mosaic Technologies Multiple sequential polynucleotide displacement reactions for signal amplification and processing
CA2313641A1 (en) * 1997-12-12 1999-06-24 Digene Corporation Universal collection medium
US20100105572A1 (en) * 1997-12-19 2010-04-29 Kris Richard M High throughput assay system
WO2000020643A1 (en) 1998-10-05 2000-04-13 Mosaic Technologies Reverse displacement assay for detection of nucleic acid sequences
JP3267576B2 (ja) * 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
US6432642B1 (en) 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
AU5103300A (en) * 1999-06-07 2000-12-28 Kenshi Obaru Primers for polymerase reactions
CA2404431C (en) 2000-03-27 2011-06-07 Thomas Jefferson University Guanylyl cyclase c in the detection of stomach and esophageal cancers
KR100446358B1 (ko) * 2000-03-31 2004-09-01 산코 준야쿠 가부시키가이샤 프로우브 중합체 제작용 프로우브, 프로우브 중합체의 제작방법 및 그 이용
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7829276B2 (en) * 2000-09-18 2010-11-09 Thomas Jefferson University Methods of using CRCA-1 as a stomach and esophageal cancer marker
ES2273892T3 (es) * 2000-10-11 2007-05-16 Sanko Junyaku Co., Ltd. Procedimiento de construccion de sondas de autoensamblaje y procedimiento de deteccion de las mismas.
EP1401850A1 (de) 2001-06-20 2004-03-31 Nuevolution A/S Nukleosidderivate zur herstellung einer verbindungsbibliothek
CN101074450A (zh) 2001-09-24 2007-11-21 一兰姆达公司 诊断探针检测系统
US20050130139A1 (en) * 2001-09-28 2005-06-16 Mitsugu Usui Method f amplifying dna chip signals
US20040029142A1 (en) * 2002-02-11 2004-02-12 Schon Eric A. Concatenation-based nucleic acid detection compositions and methods
CA2433473A1 (en) * 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
EP1539980B1 (de) 2002-08-01 2016-02-17 Nuevolution A/S MEHRSTUFENSYNTHESE VON MIT MATRIZEN ASSOZIIERTE MOLEKüLEN
EP2348124B1 (de) * 2002-10-30 2013-12-11 Nuevolution A/S Synthese bifunktioneller Komplexe
WO2004056994A2 (en) * 2002-12-19 2004-07-08 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US20070026397A1 (en) 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
EP1611254B1 (de) 2003-03-31 2014-09-10 Roche Diagnostics GmbH Zusammensetzungen und verfahren zum nachweis bestimmter flaviviren, einschliesslich mitgliedern der japanese-encephalitis-virus-serogruppe
US7514551B2 (en) 2003-04-03 2009-04-07 Enzo Life Sciences, Inc. Multisignal labeling reagents, and processes and uses therefor
US9696298B2 (en) 2003-04-03 2017-07-04 Enzo Life Sciences, Inc. Multisignal reagents for labeling analytes
US9156986B2 (en) 2003-04-03 2015-10-13 Enzo Life Sciences, Inc. Multisignal labeling reagents and processes and uses therefor
US11118215B2 (en) 2003-09-18 2021-09-14 Nuevolution A/S Method for obtaining structural information concerning an encoded molecule and method for selecting compounds
US7306915B2 (en) * 2004-03-22 2007-12-11 Sysmex Corporation Probe set for detection of target substance and detection method using the same
US7745124B2 (en) * 2004-04-28 2010-06-29 Eisai R&D Management Co., Ltd. Hybridization method
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
DE602005020319D1 (de) * 2004-09-08 2010-05-12 Eisai R&D Man Co Ltd Verfahren zur bildung eines signalsondenpolymers
US7842794B2 (en) 2004-12-17 2010-11-30 Roche Molecular Systems, Inc. Reagents and methods for detecting Neisseria gonorrhoeae
US20070048759A1 (en) * 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
PT3305900T (pt) 2005-12-01 2021-10-27 Nuevolution As Métodos de codificação enzimática para síntese eficaz de bibliotecas grandes
WO2007095154A2 (en) * 2006-02-10 2007-08-23 The Brigham And Women's Hospital, Inc. Lectin complement pathway assays and related compositions and methods
US20070275388A1 (en) * 2006-05-25 2007-11-29 Daniel Ryan Dendrimers that possess a single target annealing site and uses thereof
EP2056709A4 (de) 2006-08-11 2013-05-01 Univ New Jersey Med Zweifachsensibilisator mit lumineszenzverbindungen, konjugaten und verwendung
EP2180063B1 (de) * 2007-08-14 2013-01-09 Eisai R&D Management Co., Ltd. Verfahren zur erkennung einer zielsubstanz
SG185315A1 (en) * 2007-10-24 2012-11-29 Eisai R&D Man Co Ltd Nucleic acid probe, and method of forming probe -polymer
KR20100042082A (ko) * 2008-10-15 2010-04-23 삼성전자주식회사 신호물질의 밀도가 증가되어 있는 고체 지지체, 그를 포함하는 키트 및 그를 이용한 표적물질 검출 방법
TWI419974B (zh) * 2008-10-27 2013-12-21 Qiagen Gaithersburg Inc 於自動平台上之快速結果雜交捕捉法
ES2644516T3 (es) * 2009-01-28 2017-11-29 Qiagen Gaithersburg, Inc. Método y ensayo de preparación de muestras de gran volumen específico de secuencia
WO2010127228A1 (en) * 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of rna splice-forms in a sample
US20100316998A1 (en) * 2009-06-15 2010-12-16 Massachusetts Institute Of Technology Methods and compositions for empirical selection of drugs or other molecules
JP5826752B2 (ja) 2009-09-14 2015-12-02 キアジェン ゲイサーズバーグ インコーポレイテッド 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法
US9221759B2 (en) 2010-01-13 2015-12-29 Rutgers, The State University Of New Jersey Fluorophore chelated lanthanide luminescent probes with improved quantum efficiency
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
CA2787924A1 (en) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
EP2572001A2 (de) 2010-05-19 2013-03-27 QIAGEN Gaithersburg, Inc. Verfahren und zusammensetzungen für sequenzspezifische reinigung und multiplex-analyse von nukleinsäuren
JP6153866B2 (ja) 2010-05-25 2017-06-28 キアゲン ガイサーズバーグ アイエヌシー． 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ
US9777320B2 (en) * 2010-08-25 2017-10-03 Pacific Biosciences Of California, Inc. Molecular adaptors for dye conjugates
WO2012116220A2 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
US10385392B2 (en) 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes
WO2014145620A2 (en) * 2013-03-15 2014-09-18 The Broad Institute, Inc. Novel hybridization probes and uses thereof
CN105722977A (zh) 2013-10-30 2016-06-29 绿色生活生物技术有限责任公司 用于柑橘绿化枯萎病的发病定量系统和治疗方法
DK3555313T3 (da) * 2016-12-16 2021-08-09 Aratome Llc Molekylær detektering ved hjælp af ligeringsamplifikation
EP3696275B1 (de) * 2017-10-11 2025-12-24 MGI Tech Co., Ltd. Verfahren zur verbesserung der belastung und stabilität von nukleinsäuren auf einem festen träger
CN114544966B (zh) * 2020-11-11 2023-05-09 艾克发(北京)生物技术有限公司 一种多重信号放大系统及其在免疫吸附夹心法检测中的应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4762780A (en) * 1984-04-17 1988-08-09 The Regents Of The University Of California Method and composition for screening and diagnosing "HCMV"
US4882669A (en) * 1983-11-28 1989-11-21 Canon Kabushiki Kaisha Multi computer fail safe control apparatus
US4724202A (en) * 1983-12-12 1988-02-09 Molecular Diagnostics, Inc. Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization
FI71768C (fi) * 1984-02-17 1987-02-09 Orion Yhtymae Oy Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning.
GB8405437D0 (en) * 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
DE3420925A1 (de) * 1984-06-05 1985-12-05 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zum nachweis einer nukleinsaeure-sequenz
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
EP0204510B1 (de) * 1985-05-31 1991-07-10 Amoco Corporation Verstärkung von Hybridisierungssignalen durch Verwendung von komplementarischen DNS-Strängen
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
EP0245206A1 (de) * 1986-05-05 1987-11-11 IntraCel Corporation Analytisches Verfahren zum Nachweis und zur Messung einer spezifisch sequentierten Nukleinsäure
IL86164A0 (en) * 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
CA1339351C (en) * 1987-10-15 1997-08-26 Michael S. Urdea Nucleic acid multimers and amplified nucleic acid hybridization assays using same

Also Published As

Publication number Publication date
EP0450594B1 (de) 1996-12-18
JPH0788000A (ja) 1995-04-04
JP2000279186A (ja) 2000-10-10
DE69123621T2 (de) 1997-04-17
EP0450594A3 (en) 1992-01-22
US5437977A (en) 1995-08-01
CA2039517C (en) 2006-11-07
JP3142888B2 (ja) 2001-03-07
ES2097768T3 (es) 1997-04-16
DE69123621D1 (de) 1997-01-30
CA2039517A1 (en) 1991-10-04
EP0450594A2 (de) 1991-10-09

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