ES2103559T3 - Inmunoensayo de dos sitios para un anticuerpo con marcado luminiscente y ligando unido a biotina. - Google Patents

Inmunoensayo de dos sitios para un anticuerpo con marcado luminiscente y ligando unido a biotina.

Info

Publication number
ES2103559T3
ES2103559T3 ES94900067T ES94900067T ES2103559T3 ES 2103559 T3 ES2103559 T3 ES 2103559T3 ES 94900067 T ES94900067 T ES 94900067T ES 94900067 T ES94900067 T ES 94900067T ES 2103559 T3 ES2103559 T3 ES 2103559T3
Authority
ES
Spain
Prior art keywords
antibody
linked
solid phase
biotine
sites
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94900067T
Other languages
English (en)
Inventor
Niels Johansen
Hans-Henrik Ipsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ALK Abello AS
Original Assignee
ALK AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ALK AS filed Critical ALK AS
Application granted granted Critical
Publication of ES2103559T3 publication Critical patent/ES2103559T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

UN METODO DE DETECTAR UN ANTICUERPO EN UNA MUESTRA QUE USA UN COMPUESTO DE ETIQUETADO, Y COMPRENDIENDO LAS FASES DE MEZCLAR UN LIGANDO ANTIGENO, ANTICUERPO O "HAPTEN" (ANTIGENO PARCIAL INCAPAZ DE ESTIMULAR LA PRODUCCION DE ANTICUERPOS CUANDO SE INYECTA A UN ANIMAL, Y QUE REACCIONA CON UN ANTICUERPO TAN SOLO DENTRO DE UN MEDIO AMBIENTE), COMBINADO A BIOTIN CON LA MUESTRA; UN ANTICUERPO DIRIGIDO CONTRA EL ANTICUERPO A DETECTAR UNIDO A PARTICULAS PARAMAGNETICAS; Y UN COMPUESTO DE ACRIDINIO QUIMIOLUMINISCENTE UNIDO A AVIDIN O ESTREPTAVIDIN, PARA FORMAR UN COMPLEJO DE FASE SOLIDA; SEPARANDO LA FASE SOLIDA DE LA FASE LIQUIDA; Y ANALIZANDO LA FASE SOLIDA SEPARADA, A EFECTOS DE LA PRESENCIA DEL COMPLEJO QUIMIOLULINISCENTE.
ES94900067T 1992-11-13 1993-11-15 Inmunoensayo de dos sitios para un anticuerpo con marcado luminiscente y ligando unido a biotina. Expired - Lifetime ES2103559T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK921379A DK137992D0 (da) 1992-11-13 1992-11-13 Fremgangsmaade til paavisning af et immunologisk aktivt stof i en proeve under anvendelse af et maerkningsstof

Publications (1)

Publication Number Publication Date
ES2103559T3 true ES2103559T3 (es) 1997-09-16

Family

ID=8104222

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94900067T Expired - Lifetime ES2103559T3 (es) 1992-11-13 1993-11-15 Inmunoensayo de dos sitios para un anticuerpo con marcado luminiscente y ligando unido a biotina.

Country Status (15)

Country Link
EP (1) EP0669000B1 (es)
JP (1) JP3194585B2 (es)
KR (1) KR100306433B1 (es)
AT (1) ATE152834T1 (es)
AU (1) AU682478B2 (es)
CA (1) CA2149340C (es)
DE (1) DE69310537T2 (es)
DK (2) DK137992D0 (es)
ES (1) ES2103559T3 (es)
FI (1) FI111194B (es)
HU (1) HU216876B (es)
NO (1) NO320170B1 (es)
PL (2) PL173033B1 (es)
RU (1) RU2132070C1 (es)
WO (1) WO1994011734A1 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2289334B (en) * 1994-05-10 1998-08-26 Molecular Light Technology Lim Enzyme linked chemiluminescent assay
GB9526599D0 (en) * 1995-12-28 1996-02-28 Sandoz Ltd Elisa test system
US6288803B1 (en) 1997-10-27 2001-09-11 Denso Corporation Hologram display
US6379909B1 (en) 1998-06-24 2002-04-30 Alk-Abello A/S Method of detecting an antibody in a liquid sample
ES2310939T3 (es) * 1998-06-24 2009-01-16 Alk-Abello A/S Metodo para detectar un anticuerpo en una muestra liquida.
US7759133B2 (en) 1998-12-22 2010-07-20 Alk-Abello A/S Method of detecting and/or quantifying a specific IgE antibody in a liquid sample
US7732157B1 (en) 1999-09-30 2010-06-08 Tumor Biology Investment Group Soluble epidermal growth factor receptor-like proteins and their uses in cancer detection methods
GB0029154D0 (en) 2000-11-30 2001-01-17 Lee Helen Signal enhancement with multiple labelled-antibodies
RU2195668C2 (ru) * 2001-02-12 2002-12-27 Мешандин Алексей Гаврилович Способ постановки реакции агглютинационного иммунологического анализа
RU2218411C2 (ru) * 2001-12-27 2003-12-10 Ставропольский научно-исследовательский противочумный институт Способ обнаружения патогенных микроорганизмов в объектах внешней среды
US7867715B2 (en) 2003-08-05 2011-01-11 Alk-Abello A/S Method of evaluating the immunological activity of a vaccine
DE202008006598U1 (de) 2008-04-11 2008-10-02 Alk-Abelló A/S Allergie-Impfstoff-Formulierung zur mucosalen Verabreichung
RU2408734C1 (ru) * 2009-05-28 2011-01-10 Владимир Михайлович Воробейчиков Способ и устройство для обнаружения патогенных микроорганизмов
US20240151710A1 (en) * 2019-10-17 2024-05-09 Monash University Methods for detecting immune response
WO2022256568A1 (en) * 2021-06-02 2022-12-08 Arizona Board Of Regents On Behalf Of The University Of Arizona Methods and compositions for localization of growth factors
CN121703409A (zh) * 2026-02-11 2026-03-20 杭州星源华青生物科技有限公司 一种基于吖啶酯化学发光的信号放大体系和试剂盒

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1612264A1 (ru) * 1989-03-01 1990-12-07 Институт биоорганической химии им.М.М.Шемякина Способ твердофазного иммуноферментного определени антител к вирусу иммунодефицита человека и меченный биотином синтетический пептид дл его осуществлени

Also Published As

Publication number Publication date
FI111194B (fi) 2003-06-13
HU216876B (hu) 1999-09-28
FI952330A0 (fi) 1995-05-12
CA2149340A1 (en) 1994-05-26
FI952330L (fi) 1995-05-12
JPH08503071A (ja) 1996-04-02
JP3194585B2 (ja) 2001-07-30
EP0669000B1 (en) 1997-05-07
RU2132070C1 (ru) 1999-06-20
AU5462594A (en) 1994-06-08
DE69310537T2 (de) 1997-11-27
PL173033B1 (pl) 1998-01-30
NO951875D0 (no) 1995-05-11
ATE152834T1 (de) 1997-05-15
NO320170B1 (no) 2005-11-07
KR100306433B1 (ko) 2001-11-30
AU682478B2 (en) 1997-10-09
WO1994011734A1 (en) 1994-05-26
HUT71780A (en) 1996-02-28
KR950704686A (ko) 1995-11-20
PL309004A1 (en) 1995-09-18
NO951875L (no) 1995-05-11
PL173049B1 (pl) 1998-01-30
DE69310537D1 (de) 1997-06-12
EP0669000A1 (en) 1995-08-30
DK0669000T3 (da) 1997-12-08
CA2149340C (en) 2005-01-11
DK137992D0 (da) 1992-11-13

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