ES2104160T3 - Metodo para introducir secuencias definidas en el extremo 3' de polinucleotidos. - Google Patents

Metodo para introducir secuencias definidas en el extremo 3' de polinucleotidos.

Info

Publication number
ES2104160T3
ES2104160T3 ES93917347T ES93917347T ES2104160T3 ES 2104160 T3 ES2104160 T3 ES 2104160T3 ES 93917347 T ES93917347 T ES 93917347T ES 93917347 T ES93917347 T ES 93917347T ES 2104160 T3 ES2104160 T3 ES 2104160T3
Authority
ES
Spain
Prior art keywords
polynucleotide
primer
sequences
sequence
blocker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93917347T
Other languages
English (en)
Inventor
Maureen Laney
Yan Chen
Edwin F Ullman
Karen N Hahnenberger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Application granted granted Critical
Publication of ES2104160T3 publication Critical patent/ES2104160T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

SE PRESENTA UN METODO PARA EXTENDER UN INICIADOR Y ASI PRODUCIR UN POLIDESOXINUCLEOTIDO DE UNA SOLA CADENA QUE TENGA DOS O MAS SECUENCIAS DEFINIDAS. SE PRESENTA UNA COMBINACION QUE CONTIENE UN POLINUCLEOTIDO PATRON, UN POLINUCLEOTIDO BLOQUEADOR, UN POLINUCLEOTIDO INICIADOR Y UN POLINUCLEOTIDO Q. EL POLINUCLEOTIDO PATRON TIENE TRES SECUENCIAS T1, T2 Y T3 EN DONDE T1 NO ES CONTIGUA Y 3'' DE T3 Y EN DONDE EL TERMINA 5'' DE T3 ES 5'' DEL TERMINAL 5'' DE T2. EL POLINUCLEOTIDO INICIADOR TIENE UNA SEGUNDA SECUENCIA DEFINIDA EN SU TERMINAL 3'' QUE SE PUEDE HIBRIDAR CON T1. EL POLINUCLEOTIDO BLOQUEADOR TIENE LA SECUENCIA B1 QUE SE PUEDE HIBRIDAR CON T3. EL POLINUCLEOTIDO Q TIENE SECUENCIAS S1 Y S2 EN DONDE S1 ES 3'' DE S2 Y HOMOLOGA A T2 Y S2 ES COMPLEMENTARIA A UNA PRIMERA SECUENCIA DEFINIDA QUE SE VA A INTRODUCIR EN EL EXTREMO 3'' DEL INICIADOR CUANDO SE EXTIENDE DURANTE EL METODO DE LA INVENCION. EL POLINUCLEOTIDO Q SE ENCUENTRA BIEN UNIDO AL TERMINAL 5'' DEL POLINUCLEOTIDO BLOQUEADOR O BIEN SE ENCUENTRAPRESENTE COMO UN REACTIVO INDEPENDIENTE. EL INICIADOR SE EXTIENDE A LO LARGO DEL POLINUCLEOTIDO PATRON Y A LO LARGO DE AL MENOS UNA PORCION DE LA SECUENCIA T2 Y DESPUES A LO LARGO DEL POLINUCLEOTIDO Q PARA ASI OBTENER UN POLINUCLEOTIDO DE UNA SOLA CADENA CON DOS O MAS SECUENCIAS DEFINIDAS.
ES93917347T 1992-07-31 1993-07-30 Metodo para introducir secuencias definidas en el extremo 3' de polinucleotidos. Expired - Lifetime ES2104160T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US92307992A 1992-07-31 1992-07-31

Publications (1)

Publication Number Publication Date
ES2104160T3 true ES2104160T3 (es) 1997-10-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES93917347T Expired - Lifetime ES2104160T3 (es) 1992-07-31 1993-07-30 Metodo para introducir secuencias definidas en el extremo 3' de polinucleotidos.

Country Status (9)

Country Link
US (3) US5679512A (es)
EP (1) EP0652973B1 (es)
JP (1) JPH07509365A (es)
AT (1) ATE152180T1 (es)
CA (1) CA2141450A1 (es)
DE (1) DE69310179T2 (es)
DK (1) DK0652973T3 (es)
ES (1) ES2104160T3 (es)
WO (1) WO1994003637A1 (es)

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CA2141450A1 (en) 1994-02-17
WO1994003637A1 (en) 1994-02-17
EP0652973B1 (en) 1997-04-23
DK0652973T3 (da) 1997-09-15
US6030774A (en) 2000-02-29
EP0652973A1 (en) 1995-05-17
ATE152180T1 (de) 1997-05-15
DE69310179T2 (de) 1997-07-31
DE69310179D1 (de) 1997-05-28
JPH07509365A (ja) 1995-10-19
US5683879A (en) 1997-11-04
US5679512A (en) 1997-10-21

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