ES2110604T3 - Metodo de secuenciacion de adn. - Google Patents

Metodo de secuenciacion de adn.

Info

Publication number
ES2110604T3
ES2110604T3 ES93909381T ES93909381T ES2110604T3 ES 2110604 T3 ES2110604 T3 ES 2110604T3 ES 93909381 T ES93909381 T ES 93909381T ES 93909381 T ES93909381 T ES 93909381T ES 2110604 T3 ES2110604 T3 ES 2110604T3
Authority
ES
Spain
Prior art keywords
pct
dna sequencing
sequencing method
sec
date
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93909381T
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English (en)
Inventor
Andre Rosenthal
Sydney Brenner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2110604T3 publication Critical patent/ES2110604T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE PRESENTA UN METODO PARA DETERMINAR LA SECUENCIA DE UN ACIDO NUCLEICO QUE CONSISTE EN LOS PASOS SIGUIENTES: A) FORMAR UNA MUESTRA DE UNA SOLA ESPECIE QUE CONTENGA EL ACIDO NUCLEICO A SECUENCIAR; B) HIBRIDAR UN IMPRIMADOR EN LA PLANTILLA PARA FORMAR UN COMPLEJO PLANTILLA/IMPRIMADOR; C) EXTENDER EL IMPRIMADOR MEDIANTE LA ADICION DE UN NUCLEOTIDO ETIQUETADO CON UNA SOLA ETIQUETA; D) DETERMINAR EL TIPO DE NUCLEOTIDO ETIQUETADO AÑADIDO AL IMPRIMADOR; E) RETIRAR O NEUTRALIZAR LA ETIQUETA; Y F) REPETIR LOS PASOS DEL (C) AL (E) SECUENCIALMENTE Y REGISTRAR EL ORDEN DE INCORPORACION DE LOS NUCLEOTIDOS INCORPORADOS. TAMBIEN SE PRESENTA UN APARATO PARA LLEVAR A CABO EL METODO.
ES93909381T 1992-04-22 1993-04-22 Metodo de secuenciacion de adn. Expired - Lifetime ES2110604T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB929208733A GB9208733D0 (en) 1992-04-22 1992-04-22 Dna sequencing method

Publications (1)

Publication Number Publication Date
ES2110604T3 true ES2110604T3 (es) 1998-02-16

Family

ID=10714401

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93909381T Expired - Lifetime ES2110604T3 (es) 1992-04-22 1993-04-22 Metodo de secuenciacion de adn.

Country Status (10)

Country Link
US (1) US6087095A (es)
EP (1) EP0640146B1 (es)
JP (1) JPH07507681A (es)
AT (1) ATE159766T1 (es)
AU (1) AU4020893A (es)
CA (1) CA2133956C (es)
DE (1) DE69314951T2 (es)
ES (1) ES2110604T3 (es)
GB (1) GB9208733D0 (es)
WO (1) WO1993021340A1 (es)

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US6087095A (en) 2000-07-11
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CA2133956A1 (en) 1993-10-28
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EP0640146A1 (en) 1995-03-01
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