ES2119241T3 - Procedimiento para la obtencion de insulina con puentes de cistina unidos correctamente. - Google Patents

Procedimiento para la obtencion de insulina con puentes de cistina unidos correctamente.

Info

Publication number
ES2119241T3
ES2119241T3 ES95101748T ES95101748T ES2119241T3 ES 2119241 T3 ES2119241 T3 ES 2119241T3 ES 95101748 T ES95101748 T ES 95101748T ES 95101748 T ES95101748 T ES 95101748T ES 2119241 T3 ES2119241 T3 ES 2119241T3
Authority
ES
Spain
Prior art keywords
insulin
procedure
cystine bridges
correctly
united
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95101748T
Other languages
English (en)
Inventor
Rainer Dr Obermeier
Martin Dr Gerl
Jurgen Ludwig
Walter Sabel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Application granted granted Critical
Publication of ES2119241T3 publication Critical patent/ES2119241T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

SE DESCRIBE UN PROCESO PARA LA OBTENCION DE INSULINA CON PUENTES DE CISTINA UNIDOS CORRECTAMENTE A PARTIR DE UNA CADENA DE ACIDO AMINO PROINSULINA CORRESPONDIENTE EN PRESENCIA DE MERCAPTANO, SUSTANCIAS AUXILIARES Y UN VALOR DE PH DESDE 10 HASTA 11, ASI COMO LA TRANSFORMACION DIRECTA DE LA PROINSULINA A INSULINA CON LA AYUDA DE ENZIMAS Y AISLAMIENTO DIRECTO DE LA INSULINA A PARTIR DEL CONJUNTO DE REACCION CON LA AYUDA DE RESINA ABSORBEDORA HIDROFOBA.
ES95101748T 1994-02-18 1995-02-09 Procedimiento para la obtencion de insulina con puentes de cistina unidos correctamente. Expired - Lifetime ES2119241T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4405179A DE4405179A1 (de) 1994-02-18 1994-02-18 Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken

Publications (1)

Publication Number Publication Date
ES2119241T3 true ES2119241T3 (es) 1998-10-01

Family

ID=6510552

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95101748T Expired - Lifetime ES2119241T3 (es) 1994-02-18 1995-02-09 Procedimiento para la obtencion de insulina con puentes de cistina unidos correctamente.

Country Status (14)

Country Link
US (1) US5663291A (es)
EP (1) EP0668292B1 (es)
JP (1) JP3863579B2 (es)
KR (1) KR100371824B1 (es)
AT (1) ATE166069T1 (es)
AU (1) AU699817B2 (es)
CA (1) CA2142780C (es)
DE (2) DE4405179A1 (es)
DK (1) DK0668292T3 (es)
ES (1) ES2119241T3 (es)
FI (1) FI115916B (es)
IL (1) IL112680A0 (es)
NO (1) NO318424B1 (es)
SG (1) SG46683A1 (es)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE59711533D1 (de) 1996-07-26 2004-05-27 Aventis Pharma Gmbh Insulinderivate mit erhöhter Zinkbindung
DE19726167B4 (de) 1997-06-20 2008-01-24 Sanofi-Aventis Deutschland Gmbh Insulin, Verfahren zu seiner Herstellung und es enthaltende pharmazeutische Zubereitung
DE19735711C2 (de) 1997-08-18 2001-04-26 Aventis Pharma Gmbh Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
DE19825447A1 (de) * 1998-06-06 1999-12-09 Hoechst Marion Roussel De Gmbh Neue Insulinanaloga mit erhöhter Zinkbildung
DE19838097A1 (de) 1998-08-24 2000-03-02 Hoechst Marion Roussel De Gmbh Verfahren zur chromatographischen Reinigung von Insulinen
FI107115B (fi) 1998-09-30 2001-06-15 Valio Oy Menetelmä naudan insuliinin poistamiseksi
EP0994188B1 (de) * 1998-10-09 2004-01-07 Scil proteins GmbH Verfahren zur Gewinnung von aktivem Beta-NGF
JP3406244B2 (ja) 1999-04-30 2003-05-12 伊藤ハム株式会社 新規な融合蛋白質からの組み換えインスリンの製造方法
US6746853B1 (en) 1999-05-19 2004-06-08 Xencor, Inc. Proteins with insulin-like activity useful in the treatment of diabetes
DE19930676B4 (de) * 1999-07-02 2006-01-19 Sanofi-Aventis Deutschland Gmbh Verfahren zur Stabilisierung von Insulin, Insulinderivaten und/oder deren Vorläufer in komplexen Mischungen bei deren Lagerung in wäßrigen Lösungsmitteln
AR025646A1 (es) * 2000-09-13 2002-12-04 Beta Lab Sa Cepa de levaduras metilotroficas recombinantes productoras de un precursor de insulina, construcciones de adn y metodo para obtener la cepa.
DE10114178A1 (de) 2001-03-23 2002-10-10 Aventis Pharma Gmbh Zinkfreie und zinkarme Insulinzubereitungen mit verbesserter Stabilität
WO2002100887A2 (en) * 2001-06-08 2002-12-19 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogs
DE10227232A1 (de) * 2002-06-18 2004-01-15 Aventis Pharma Deutschland Gmbh Saure Insulinzubereitungen mit verbesserter Stabilität
US7193035B2 (en) * 2002-10-29 2007-03-20 Sanofi-Aventis Deutschland Gmbh Crystals of insulin analogs and processes for their preparation
ES2337684T3 (es) * 2003-12-05 2010-04-28 F. Hoffmann-La Roche Ag Carboxipeptidasa b recombinante y su purificacion.
DE102004015965A1 (de) 2004-04-01 2005-10-20 Aventis Pharma Gmbh Verfahren zur Gewinnung von Insulinen durch verbesserte Luftbegasung der Faltung
DE102006031962A1 (de) * 2006-07-11 2008-01-17 Sanofi-Aventis Deutschland Gmbh Amidiertes Insulin Glargin
DE102006031955A1 (de) 2006-07-11 2008-01-17 Sanofi-Aventis Deutschland Gmbh Verfahren zur Herstellung von Insulinanaloga mit dibasischem B-Kettenende
US8802816B2 (en) * 2008-02-19 2014-08-12 Biocon Limited Method of obtaining a purified, biologically active heterologous protein
US9296806B2 (en) * 2008-04-30 2016-03-29 Wockhardt Limited Processes for refolding of insulin
PL219335B1 (pl) * 2008-07-04 2015-04-30 Inst Biotechnologii I Antybiotyków Pochodna insuliny lub jej farmaceutycznie dopuszczalna sól, jej zastosowanie oraz zawierająca ją kompozycja farmaceutyczna
PT3228320T (pt) 2008-10-17 2020-03-26 Sanofi Aventis Deutschland Combinação de uma insulina e de um agonista do glp-1
UA91281C2 (ru) * 2008-11-26 2010-07-12 Общество С Ограниченной Ответственностью «Мако» Способ получения рекомбинантного инсулина человека
KR101853606B1 (ko) 2009-07-06 2018-05-03 사노피-아벤티스 도이칠란트 게엠베하 메티오닌을 함유하는 수성 인슐린 제제
DK2498802T3 (en) 2009-11-13 2015-04-13 Sanofi Aventis Deutschland Pharmaceutical composition comprising a GLP-1 agonist, insulin and a methionine
KR101337322B1 (ko) 2009-11-13 2013-12-06 사노피-아벤티스 도이칠란트 게엠베하 Ｇｌｐ-1 효능제 및 메티오닌을 포함하는 약제학적 조성물
ES2606554T3 (es) 2010-08-30 2017-03-24 Sanofi-Aventis Deutschland Gmbh Uso de AVE0010 para la fabricación de un medicamento para el tratamiento de la diabetes mellitus de tipo 2
JP6010108B2 (ja) 2011-04-01 2016-10-19 スリーエム イノベイティブ プロパティズ カンパニー トリアジン系紫外線吸収剤を含むフィルム
PL2739646T3 (pl) * 2011-05-10 2018-05-30 Stefan Hermann Kompleksowy układ buforowy do renaturacji ludzkiej proinsuliny lub pochodnych proinsuliny
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
PL2750699T3 (pl) 2011-08-29 2015-12-31 Sanofi Aventis Deutschland Kombinacja farmaceutyczna do stosowania w kontroli glikemii u pacjentów z cukrzycą typu 2
AR087744A1 (es) 2011-09-01 2014-04-16 Sanofi Aventis Deutschland Composicion farmaceutica para uso en el tratamiento de una enfermedad neurodegenerativa
US10421795B2 (en) 2012-12-17 2019-09-24 Merck Sharp & Dohme Corp. Process for purifying insulin and analogues thereof
WO2015138548A1 (en) 2014-03-14 2015-09-17 Merck Sharp & Dohme Corp. Purifying insulin using cation exchange and reverse phase chromatography in the presence of an organic modifier and elevated temperature
AU2015359376B2 (en) 2014-12-12 2021-09-09 Sanofi-Aventis Deutschland Gmbh Insulin glargine/lixisenatide fixed ratio formulation
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療
EP3344651B1 (en) 2015-09-02 2022-03-02 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ196609A (en) * 1980-03-27 1984-02-03 Lilly Co Eli Preparation of proinsulin-like insulin precursors
NZ199391A (en) * 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
US4616078A (en) * 1985-04-08 1986-10-07 Eli Lilly And Company Process for purifying proinsulin-like materials
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US4877868A (en) * 1986-03-12 1989-10-31 Neorx Corporation Radionuclide antibody coupling
DE4012818A1 (de) * 1990-04-21 1991-10-24 Hoechst Ag Verfahren zur herstellung von fremdproteinen in streptomyceten
DE58906966D1 (de) * 1988-06-23 1994-03-24 Hoechst Ag Mini-Proinsulin, seine Herstellung und Verwendung.
DE3901718A1 (de) * 1989-01-21 1990-07-26 Hoechst Ag Verfahren zur renaturierung falscher rekombinanten von insulinvorlaeufern
IL95495A (en) * 1989-08-29 1996-10-16 Hoechst Ag Fusion proteins their preparation and use
DE4028120C2 (de) * 1990-09-05 1996-09-19 Hoechst Ag Verfahren zur Reinigung von Insulin und/oder Insulinderivaten
US5231178A (en) * 1991-01-16 1993-07-27 The Salk Institute Biotechnology/Industrial Associates, Inc. Method for the purification of intact, correctly-folded insulin-like growth factor-1
EP0599303A3 (en) * 1992-11-27 1998-07-29 Takeda Chemical Industries, Ltd. Peptide conjugate
SG46612A1 (en) * 1992-12-02 1998-02-20 Hoechst Ag Process for obtaining proinsulin in processing correctly linked cystine bridges
US5523293A (en) * 1994-05-25 1996-06-04 Iowa State University Research Foundation, Inc. Soy protein-based thermoplastic composition for preparing molded articles

Also Published As

Publication number Publication date
SG46683A1 (en) 1998-02-20
JPH07265092A (ja) 1995-10-17
CA2142780C (en) 2008-10-14
HK1010457A1 (en) 1999-06-17
FI950699A0 (fi) 1995-02-16
JP3863579B2 (ja) 2006-12-27
KR100371824B1 (ko) 2003-04-10
US5663291A (en) 1997-09-02
ATE166069T1 (de) 1998-05-15
AU1228895A (en) 1995-08-31
EP0668292A2 (de) 1995-08-23
FI950699L (fi) 1995-08-19
AU699817B2 (en) 1998-12-17
DK0668292T3 (da) 1998-11-23
EP0668292A3 (de) 1996-02-07
KR950032282A (ko) 1995-12-20
FI115916B (fi) 2005-08-15
EP0668292B1 (de) 1998-05-13
DE4405179A1 (de) 1995-08-24
NO950592L (no) 1995-08-21
NO318424B1 (no) 2005-04-18
DE59502138D1 (de) 1998-06-18
CA2142780A1 (en) 1995-08-19
IL112680A0 (en) 1995-05-26
NO950592D0 (no) 1995-02-17

Similar Documents

Publication Publication Date Title
ES2097426T3 (es) Procedimiento para la obtencion de proinsulina con puentes de cistina correctamente unidos.
CA2142780A1 (en) Process for obtaining insulin having correctly linked cystine bridges
ES2124373T3 (es) Procedimiento para la preparacion de derivados del acido n-acil-n,n',n'-etilendiamintriacetico.
GB2301363A (en) Human phosphodiesterase type ivc and its production and use
AU7832991A (en) Ribosome inactivating proteins, inactive precursor forms thereof, a process for making and a method of using
SE8204627D0 (sv) Process for preparing insulin derivatives
TW344662B (en) A process for preparing a freeze dried thiotepa composition
PT86552A (de) Gama-glutamyl-transpeptidase deren herstellung und deren verwendung
NZ503372A (en) Chemical supplementation of bone by exposing bone to a therapeutic compound and applying a potential difference across the bone or tissue
ES2136724T3 (es) Emulsion liofilizada, que contiene una sustancia activa.
ATE50595T1 (de) 2-oxocarbonsaeurereduktase, ihre herstellung und verwendung.
ES2016227A4 (es) 54- procedimiento para la elaboracion de aminoacidos l-alfa.
ATE152094T1 (de) N-geschützte (s)-isoserin-verbindungen
AU5982299A (en) Novel process for the cryo-preservation of plants
ES2058224T3 (es) Procedimiento para la hidrolisis enzimatica de compuestos alfa-aminoadipinil-monoaminicos.
GR77912B (es)
UA10180C2 (uk) Спосіб одержання біологічно активної речовини, що має регенераторну та модулюючу дію
DE59501436D1 (de) Verfahren zur Herstellung von 2-Cyaniminothiazolidin
PT879603E (pt) Antagonista de canais de calcio possuindo uma accao inibidora contra a secrecao da hormona paratiroideia e a sua utilizacao
ATE249467T1 (de) Herstellung von optisch aktiven halohydrintrialkylsilylethern
GB1515487A (en) Process for the preparation of silage
ES292976U (es) Inyector de fuelle para anestesia femenina

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 668292

Country of ref document: ES