ES2124694T3 - Clonacion de la n-metil-hidantoinasa. - Google Patents

Clonacion de la n-metil-hidantoinasa.

Info

Publication number
ES2124694T3
ES2124694T3 ES91111155T ES91111155T ES2124694T3 ES 2124694 T3 ES2124694 T3 ES 2124694T3 ES 91111155 T ES91111155 T ES 91111155T ES 91111155 T ES91111155 T ES 91111155T ES 2124694 T3 ES2124694 T3 ES 2124694T3
Authority
ES
Spain
Prior art keywords
sequence
hydantoinase
cloning
methyl
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91111155T
Other languages
English (en)
Inventor
Gunther Dr Schumacher
Helmut Dr Burtscher
Hans Mollering
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2124694T3 publication Critical patent/ES2124694T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA INVENCION TRATA DE UN DNA QUE ESTA CODIFICADO PARA UNA PROTEINA CON ACTIVIDAD N-METILHIDANTOINA Y QUE POSEE 1) LA SECUENCIA ACIDIFICADA NUCLEAR 2) UNA SECUENCIA QUE LE CONTIENE DENTRO DEL CUADRO DE DEGENERACION DEL CODIGO GENETICO O 3) UNA SECUENCIA QUE ES HIBRIDA CON OTRA SECUENCIA DE 1) O/Y 2) BAJO CONDICIONES TRANCANILADAS. ADEMAS ESTA INVENCION TRATA TAMBIEN DE UN VECTOR RECOMBINADO QUE CONTIENE UN DNA SEGUN LA INVENCION, UNA CELULA TRANSFORMADA CON UN VECTOR ASI COMO METODO PARA OBTENER UNA PROTEINA RECOMBINADA CON NMH-ASE-ACTIVIDAD.
ES91111155T 1990-07-06 1991-07-04 Clonacion de la n-metil-hidantoinasa. Expired - Lifetime ES2124694T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4021571A DE4021571A1 (de) 1990-07-06 1990-07-06 N-methylhydantoinase, kloniert

Publications (1)

Publication Number Publication Date
ES2124694T3 true ES2124694T3 (es) 1999-02-16

Family

ID=6409795

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91111155T Expired - Lifetime ES2124694T3 (es) 1990-07-06 1991-07-04 Clonacion de la n-metil-hidantoinasa.

Country Status (10)

Country Link
US (2) US5213969A (es)
EP (1) EP0464832B1 (es)
JP (2) JP2546554B2 (es)
KR (1) KR950010819B1 (es)
AT (1) ATE173762T1 (es)
AU (1) AU628265B2 (es)
CA (1) CA2045839C (es)
DE (2) DE4021571A1 (es)
ES (1) ES2124694T3 (es)
ZA (1) ZA915211B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9216851D0 (en) * 1992-08-07 1992-09-23 Univ Manitoba Dna sequences of rat probasin gene
KR100428725B1 (ko) * 1996-03-30 2004-07-07 주식회사 현대시스콤 디지탈이동통신교환기녹음안내장치의2단계자체루프백시험장치및방법
US20080318889A1 (en) * 1999-04-08 2008-12-25 Antisoma Research Limited Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
US7960540B2 (en) * 1999-04-08 2011-06-14 Advanced Cancer Therapeutics, Llc Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
JP2002541264A (ja) 1999-04-08 2002-12-03 ユーエイビー・リサーチ・ファウンデーション 冨ｇオリゴヌクレオチドの抗増殖活性およびヌクレオリンに結合するためのその使用方法
US8114850B2 (en) * 1999-04-08 2012-02-14 Advanced Cancer Therapeutics, Llc Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
US20080318890A1 (en) * 1999-04-08 2008-12-25 Antisoma Research Limited Antiproliferative activity of G-rich oligonucleotides and method of using same to bind to nucleolin
DE10130169A1 (de) * 2001-06-22 2003-01-09 Degussa Aktivierte rec-D-Hydantoinasen
AU2003231163A1 (en) * 2002-05-01 2003-11-17 Polymer Technology Systems, Inc. Test strip and method for determining concentration of creatinine in a body fluid
US20090131351A1 (en) * 2007-11-16 2009-05-21 Antisoma Research Limited Methods, compositions, and kits for modulating tumor cell proliferation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3406770A1 (de) * 1984-02-24 1985-08-29 Boehringer Mannheim Gmbh, 6800 Mannheim Nucleosidtriphosphat-abhaengige 1-methylhydantoinase und ihre verwendung
DE3535987A1 (de) * 1985-10-09 1987-04-23 Basf Ag Verfahren zur herstellung von meophilen mikroorganismen, die eine bei hoeherer temperatur aktive d-hydantoinase enthalten
DE3716957A1 (de) * 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten
DE4000668A1 (de) * 1990-01-11 1991-07-18 Boehringer Mannheim Gmbh Verfahren und reagenz zur enzymatischen bestimmung von creatinin

Also Published As

Publication number Publication date
ZA915211B (en) 1992-04-29
AU7949991A (en) 1992-06-04
CA2045839A1 (en) 1992-01-07
US5432070A (en) 1995-07-11
EP0464832A2 (de) 1992-01-08
EP0464832B1 (de) 1998-11-25
DE59109072D1 (de) 1999-01-07
JP2546554B2 (ja) 1996-10-23
JPH07313196A (ja) 1995-12-05
JPH04229181A (ja) 1992-08-18
EP0464832A3 (en) 1992-09-23
KR920002786A (ko) 1992-02-28
CA2045839C (en) 2001-12-18
US5213969A (en) 1993-05-25
AU628265B2 (en) 1992-09-10
DE4021571A1 (de) 1992-01-16
JP2984906B2 (ja) 1999-11-29
ATE173762T1 (de) 1998-12-15
KR950010819B1 (ko) 1995-09-23

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