ES2169143T3 - Procedimiento de secuenciacion de nucleotidos. - Google Patents

Procedimiento de secuenciacion de nucleotidos.

Info

Publication number
ES2169143T3
ES2169143T3 ES95929169T ES95929169T ES2169143T3 ES 2169143 T3 ES2169143 T3 ES 2169143T3 ES 95929169 T ES95929169 T ES 95929169T ES 95929169 T ES95929169 T ES 95929169T ES 2169143 T3 ES2169143 T3 ES 2169143T3
Authority
ES
Spain
Prior art keywords
nucleotide
pct
target sample
primers
ddntps
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95929169T
Other languages
English (en)
Inventor
Gillian Tully
Kevin Sullivan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for the Home Department
Original Assignee
UK Secretary of State for the Home Department
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UK Secretary of State for the Home Department filed Critical UK Secretary of State for the Home Department
Application granted granted Critical
Publication of ES2169143T3 publication Critical patent/ES2169143T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE PROPORCIONA UN METODO PARA DETERMINAR LA IDENTIDAD DE AL MENOS DOS BASES DE NUCLEOTIDO UNICO DISCRETO CADA UNA ADYACENTE A UNA SECUENCIA DE BASE DE NUCLEOTIDO PREDETERMINADA EN UNA MUESTRA DIANA QUE COMPRENDE UNO O MAS TIPOS DE CADENA DE POLINUCLEOTIDO, EL METODO COMPRENDIENDO LA INCORPORACION DE UN GRUPO DE CAPTURA EN LA MUESTRA DIANA E INMOVILIZACION DE LA MUESTRA DIANA POR MEDIO DEL GRUPO DE CAPTURA; MEZCLA DE LA MUESTRA DIANA CON (I) INICIADORES DE NUCLEOTIDO QUE SON COMPLEMENTARIOS A LAS SECUENCIAS DE BASE CARACTERIZADAS DE MODO QUE FORTALEZCA LAS MISMAS EN POSICIONES ADYACENTES A LAS BASES A SER IDENTIFICADAS Y (II) AL MENOS DOS TIPOS DE TRIFOSFATO DE DIDEOXINUCLEOTIDO (DDNTP), CADA TIPO MARCADO CON UN GRUPO FLUORESCENTE DISTINGUIBLE, Y (III) UNA ENZIMA DE EXTENSION DE CADENA DE NUCLEOTIDO TAL QUE EL DDNTP COMPLEMENTARIO A LAS BASES A IDENTIFICAR SON INCORPORADAS A LOS INICIADORES DE NUCLEOTIDO; EXTRAER LOS INICIADORES DE NUCLEOTIDO EXTENSIBLES, SEPARAR LOS TIPOS DE INICIADOR DE NUCLEOTIDO EXTENDIDOS BASANDOSE EN EL TAMAÑO O CARGA E IDENTIFICAR LOS DDNTPS INCORPORADOS A CADA TIPO DE INICIADOR DE NUCLEOTIDO MEDIANTE REFERENCIA A LAS CARACTERISTICAS FLUORESCENTES ASOCIADAS A LOS GRUPOS DISTINGUIBLES. SE PROPORCIONAN INICIADORES Y UN KIT DE PRUEBA PARA USO EN LA REALIZACION DEL METODO.
ES95929169T 1994-08-25 1995-08-21 Procedimiento de secuenciacion de nucleotidos. Expired - Lifetime ES2169143T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB9417211A GB9417211D0 (en) 1994-08-25 1994-08-25 Nucleotide sequencing method

Publications (1)

Publication Number Publication Date
ES2169143T3 true ES2169143T3 (es) 2002-07-01

Family

ID=10760409

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95929169T Expired - Lifetime ES2169143T3 (es) 1994-08-25 1995-08-21 Procedimiento de secuenciacion de nucleotidos.

Country Status (10)

Country Link
US (1) US5925520A (es)
EP (1) EP0777747B1 (es)
AT (1) ATE210191T1 (es)
AU (1) AU3263095A (es)
DE (1) DE69524425T2 (es)
DK (1) DK0777747T3 (es)
ES (1) ES2169143T3 (es)
GB (1) GB9417211D0 (es)
PT (1) PT777747E (es)
WO (1) WO1996006187A1 (es)

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Also Published As

Publication number Publication date
GB9417211D0 (en) 1994-10-12
DK0777747T3 (da) 2002-04-08
AU3263095A (en) 1996-03-14
ATE210191T1 (de) 2001-12-15
US5925520A (en) 1999-07-20
WO1996006187A1 (en) 1996-02-29
DE69524425D1 (de) 2002-01-17
PT777747E (pt) 2002-05-31
EP0777747B1 (en) 2001-12-05
DE69524425T2 (de) 2002-08-01
EP0777747A1 (en) 1997-06-11

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