ES2175097T3 - Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde. - Google Patents

Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde.

Info

Publication number
ES2175097T3
ES2175097T3 ES96917989T ES96917989T ES2175097T3 ES 2175097 T3 ES2175097 T3 ES 2175097T3 ES 96917989 T ES96917989 T ES 96917989T ES 96917989 T ES96917989 T ES 96917989T ES 2175097 T3 ES2175097 T3 ES 2175097T3
Authority
ES
Spain
Prior art keywords
detection
mold
formation
nucleic acids
diana
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96917989T
Other languages
English (en)
Inventor
Edwin F Ullman
Linda Marie Western
Samuel J Rose
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Dade Behring Marburg GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dade Behring Marburg GmbH filed Critical Dade Behring Marburg GmbH
Application granted granted Critical
Publication of ES2175097T3 publication Critical patent/ES2175097T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE REVELA UN METODO PARA LA DETECCION DE UNA SECUENCIA POLINUCLEOTIDICA DIANA. EL METODO COMPRENDE LA INCUBACION DE UN OLIGONUCLEOTIDO CON LA SECUENCIA POLINUCLEOTIDICA DIANA Y UNA POLIMERASA DE NUCLEOTIDOS BAJO CONDICIONES ISOTERMICAS DONDE AL MENOS UN NUCLEOTIDO SE AÑADE AL EXTREMO 3 -TERMINAL DEL OLIGONUCLEOTIDO PARA PROPORCIONAR UN OLIGONUCLEOTIDO EXTENDIDO QUE TIENE LOS NUCLEOTIDOS ADICIONALES. LA PRESENCIA DE OLIGONUCLEOTIDOS EXTENDIDOS SE DETECTA COMO UNA INDICACION DE LA PRESENCIA DE LA SECUENCIA POLINUCLEOTIDA DIANA. EL METODO TIENE PARTICULAR APLICACION PARA LA DETECCION DE ADN.
ES96917989T 1995-06-07 1996-06-05 Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde. Expired - Lifetime ES2175097T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/486,301 US5882867A (en) 1995-06-07 1995-06-07 Detection of nucleic acids by formation of template-dependent product

Publications (1)

Publication Number Publication Date
ES2175097T3 true ES2175097T3 (es) 2002-11-16

Family

ID=23931346

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96917989T Expired - Lifetime ES2175097T3 (es) 1995-06-07 1996-06-05 Deteccion de acidos nucleicos mediante formacion de un producto dependiente de un molde.

Country Status (7)

Country Link
US (1) US5882867A (es)
EP (1) EP0857218B1 (es)
JP (1) JPH11510369A (es)
CA (1) CA2223774A1 (es)
DE (1) DE69620903T2 (es)
ES (1) ES2175097T3 (es)
WO (1) WO1996040999A1 (es)

Families Citing this family (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US6872816B1 (en) 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US6090606A (en) * 1996-01-24 2000-07-18 Third Wave Technologies, Inc. Cleavage agents
US5985557A (en) * 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US7527928B2 (en) * 1996-11-29 2009-05-05 Third Wave Technologies, Inc. Reactions on a solid surface
US7195871B2 (en) * 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
US6706471B1 (en) * 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7256020B2 (en) * 1996-11-29 2007-08-14 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US7432048B2 (en) * 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US6913881B1 (en) 1996-01-24 2005-07-05 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
US7122364B1 (en) 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US6780982B2 (en) * 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
ES2375764T3 (es) 1996-11-29 2012-03-06 Third Wave Technologies, Inc. Endonucleasas fen-1, mezclas y procedimientos de escisión.
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
CA2384838C (en) 1999-09-13 2006-07-18 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7838225B2 (en) 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
AU767490B2 (en) * 1999-11-12 2003-11-13 Isis Pharmaceuticals, Inc. Method for quantitating oligonucleotides
ATE455186T1 (de) * 2000-06-26 2010-01-15 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
AU2001297524A1 (en) * 2000-11-15 2002-09-19 Third Wave Technologies, Inc. Fen endonucleases
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
CA2439074A1 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
KR20030082535A (ko) * 2001-03-09 2003-10-22 뉴젠 테크놀로지스 인코포레이티드 Ｒｎａ 서열의 증폭을 위한 방법 및 조성물
US6696254B2 (en) * 2001-11-21 2004-02-24 Kimberly-Clark Worldwide, Inc. Detection and identification of enteric bacteria
WO2003054167A2 (en) * 2001-12-20 2003-07-03 Merck & Co., Inc. Identification of novel polymorphic sites in the human mglur8 gene and uses thereof
WO2004013606A2 (en) 2002-08-02 2004-02-12 Stratatech Corporation Species specific dna detection
ATE525469T1 (de) 2002-12-18 2011-10-15 Third Wave Tech Inc Nachweis kleiner nukleinsäuren
US8206904B2 (en) 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
US7851150B2 (en) * 2002-12-18 2010-12-14 Third Wave Technologies, Inc. Detection of small nucleic acids
US7297780B2 (en) 2003-01-06 2007-11-20 Third Wave Technologies, Inc. Reactive functional groups for preparation of modified nucleic acid
KR20070121853A (ko) 2003-03-31 2007-12-27 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
JP2006523465A (ja) * 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
US7462451B2 (en) * 2004-04-26 2008-12-09 Third Wave Technologies, Inc. Compositions for modifying nucleic acids
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US7939258B2 (en) * 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US20070207455A1 (en) * 2005-11-17 2007-09-06 Third Wave Technologies, Inc. Compositions and methods for detecting an HCV-1 subtype
KR100652903B1 (ko) * 2005-12-21 2006-12-04 한국과학기술연구원 초흡수성 고분자를 함유한 제습제의 제조 방법 및 그 제조장치
US7674924B2 (en) * 2006-05-22 2010-03-09 Third Wave Technologies, Inc. Compositions, probes, and conjugates and uses thereof
EP2029774B1 (en) 2006-06-01 2015-04-01 Third Wave Technologies, Inc. Detection of nucleic acids
GB0713183D0 (en) * 2007-07-06 2007-08-15 King S College London Method
EP2183394B1 (en) * 2007-09-07 2012-12-12 Third Wave Technologies, Inc. Methods and applications for target quantification
EP2245461A1 (en) * 2008-01-29 2010-11-03 Victor Manneh High specifity immuno-amplification
US8034568B2 (en) * 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
KR101419980B1 (ko) 2008-03-15 2014-07-15 홀로직, 인크. 증폭 반응 도중에 핵산 분자를 분석하기 위한 조성물 및 방법
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
US8208909B2 (en) 2008-06-02 2012-06-26 West Corporation System, apparatus and method for availing a mobile call of address information
US20110091931A1 (en) * 2009-04-24 2011-04-21 Colby Pharmaceutical Company Methods and Kits for Determining Oxygen Free Radical (OFR) Levels in Animal and Human Tissues as a Prognostic Marker for Cancer and Other Pathophysiologies
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
WO2011084772A2 (en) 2009-12-21 2011-07-14 Northwestern University Allelic disorders caused by mutations in trpv4
US10233501B2 (en) 2010-10-19 2019-03-19 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US20150225792A1 (en) 2014-01-17 2015-08-13 Northwestern University Compositions and methods for identifying depressive disorders
US10093981B2 (en) 2010-10-19 2018-10-09 Northwestern University Compositions and methods for identifying depressive disorders
US20150218639A1 (en) 2014-01-17 2015-08-06 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
JP2014519310A (ja) 2011-02-02 2014-08-14 エクザクト サイエンシーズ コーポレイション Ｄｎａメチル化のデジタル配列分析
US20120252682A1 (en) * 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
US8808990B2 (en) 2011-05-12 2014-08-19 Exact Sciences Corporation Serial isolation of multiple DNA targets from stool
US8993341B2 (en) 2011-05-12 2015-03-31 Exact Sciences Corporation Removal of PCR inhibitors
CN103649298A (zh) 2011-05-12 2014-03-19 精密科学公司 核酸的分离
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
ES2645418T3 (es) 2011-12-22 2017-12-05 Ibis Biosciences, Inc. Amplificación de una secuencia de un ácido ribonucleico
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
AU2013205064B2 (en) 2012-06-04 2015-07-30 Gen-Probe Incorporated Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ， インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9212392B2 (en) 2012-09-25 2015-12-15 Exact Sciences Corporation Normalization of polymerase activity
ES2859645T3 (es) 2013-03-14 2021-10-04 Mayo Found Medical Education & Res Detección de neoplasia
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
WO2015042446A2 (en) 2013-09-20 2015-03-26 The Regents Of The University Of Michigan Compositions and methods for the analysis of radiosensitivity
WO2015066695A1 (en) 2013-11-04 2015-05-07 Exact Sciences Corporation Multiple-control calibrators for dna quantitation
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015089438A1 (en) 2013-12-13 2015-06-18 Northwestern University Biomarkers for post-traumatic stress states
EP4209600A1 (en) 2013-12-19 2023-07-12 Exact Sciences Corporation Synthetic nucleic acid control molecules
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
US10465248B2 (en) 2014-12-12 2019-11-05 Exact Sciences Development Company, Llc Method of characterizing ZDHHC1 DNA
WO2016094839A2 (en) 2014-12-12 2016-06-16 Exact Sciences Corporation Compositions and methods for performing methylation detection assays
WO2016160454A1 (en) 2015-03-27 2016-10-06 Exact Sciences Corporation Detecting esophageal disorders
KR20250172724A (ko) 2015-08-31 2025-12-09 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 위 신생물 검출 방법
KR20250073555A (ko) 2015-10-30 2025-05-27 이그잭트 사이언시즈 코포레이션 복합 증폭 검출 분석 및 혈장으로부터 dna의 분리 및 검출 방법
US20170321286A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by amplification of rna sequences
CN116064796A (zh) 2016-05-05 2023-05-05 精密科学公司 通过分析甲基化dna来检测肺肿瘤
ES3035263T3 (en) 2016-07-19 2025-09-01 Exact Sciences Corp Methylated control dna
AU2017300534B2 (en) 2016-07-19 2023-11-23 Exact Sciences Corporation Nucleic acid control molecules from non-human organisms
ES2956257T3 (es) 2016-09-02 2023-12-18 Mayo Found Medical Education & Res Detección de carcinoma hepatocelular
JP7289264B2 (ja) 2017-01-27 2023-06-09 エグザクト サイエンシーズ コーポレーション メチル化ｄｎａの分析による結腸腫瘍の検出
CN110431241B (zh) 2017-02-28 2024-04-19 梅约医学教育与研究基金会 检测前列腺癌
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
JP7277460B2 (ja) 2017-11-30 2023-05-19 マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ 乳癌の検出
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
JP7348603B2 (ja) 2018-04-02 2023-09-21 エニュメラ・モレキュラー・インコーポレイテッド 核酸分子を計数するための方法、システム、および組成物
US11408030B2 (en) 2018-09-10 2022-08-09 Andy Madrid Test for detecting Alzheimer's disease
WO2020154665A1 (en) 2019-01-24 2020-07-30 Mayo Foundation For Medical Education And Research Detecting endometrial cancer
EP3947718A4 (en) 2019-04-02 2022-12-21 Enumera Molecular, Inc. METHODS, SYSTEMS AND COMPOSITIONS FOR COUNTING NUCLEIC ACID MOLECULES
US20230046033A1 (en) 2019-09-16 2023-02-16 Exact Sciences Corporation Structure and temperature-dependent flap endonuclease substrates
US11702704B2 (en) 2019-10-31 2023-07-18 Mayo Foundation For Medical Education And Research Detecting ovarian cancer
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
US20220071605A1 (en) 2020-04-17 2022-03-10 Exact Sciences Development Company, Llc Sampling device and analysis of methylated dna
JP2023539128A (ja) 2020-08-19 2023-09-13 マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ 非ホジキンリンパ腫の検出
AU2021345359A1 (en) 2020-09-21 2023-05-11 Enumera Molecular, Inc. Compositions and methods for isolation of cell-free dna
EP4314286A1 (en) 2021-08-06 2024-02-07 Sansure Biotech Inc. Compositions for liquefying a viscous biological sample, combination products, liquefying agents, and kits thereof, and methods and application thereof
WO2026055641A1 (en) 2024-09-06 2026-03-12 Exact Sciences Corporation Methods and compositions for preparing biomolecule analytes from complex samples

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5008182A (en) * 1986-01-10 1991-04-16 Cetus Corporation Detection of AIDS associated virus by polymerase chain reaction
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4868104A (en) * 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
DE3864914D1 (de) * 1987-06-15 1991-10-24 Stihl Maschf Andreas Startautomatik fuer einen verbrennungsmotor, insbesondere fuer den von hand zu startenden motor eines arbeitsgeraetes.
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
GB9009903D0 (en) * 1990-05-02 1990-06-27 Ici Plc Assay for specific nucleic acid sequences
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
EP0517361A1 (en) * 1991-05-30 1992-12-09 Amoco Corporation A method for detecting and identifying pathogenic organisms using target sequences as detectors
CA2135073C (en) * 1992-05-06 2002-11-19 Daniel L. Kacian Nucleic acid sequence amplification method, composition and kit
EP0648222B1 (en) * 1992-07-02 2003-01-29 Savyon Diagnostics Ltd. Methods of single nucleotide primer extension to detect specific alleles and kits therefor
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5457027A (en) * 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
ES2114692T3 (es) * 1993-06-09 1998-06-01 Gamera Bioscience Corp Reaccion en ciclos magneticos.
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification

Also Published As

Publication number Publication date
US5882867A (en) 1999-03-16
EP0857218B1 (en) 2002-04-24
DE69620903T2 (de) 2002-11-21
JPH11510369A (ja) 1999-09-14
CA2223774A1 (en) 1996-12-19
WO1996040999A1 (en) 1996-12-19
EP0857218A1 (en) 1998-08-12
DE69620903D1 (de) 2002-05-29

Similar Documents

Publication Publication Date Title
ES2185719T3 (es) Deteccion de acidos nucleicos por formacion de un producto catalizada por diana.
MX9705945A (es) Deteccion de acidos nucleicos utilizando cuartetas g.
AU2709995A (en) Method of amplification for increasing the sensitivity of detecting nucleic acid-probe target hybrids
IL137909A0 (en) Method for detection of target nucleic acids using pcr
KR970028548A (ko) 핵산의 형광 편광도 검출방법
DK0699768T3 (da) Fremgangsmåder til quenching i opløsning af fluorescens-mærkede oligonnukleotidprober
ATE427999T1 (de) Erkennung von nukleinsaurepolymorphismen
ATE196322T1 (de) Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
PT92602A (pt) Processo de preparacao de um adn de cadeia dupla, de deteccao de sequencias de acido nucleico alvo e de amplificacao de uma sequencia de acido nucleico alvo
DE69432419D1 (de) Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
FI933870A0 (fi) Nukleinsyratypprovning genom polymerasfoerlaengning av oligonukleotider under anvaendning av terminatorblandningar
DE60143960D1 (de) Äuren
BR9908560A (pt) Detecção de microorganismos relacionados à fermentação
ATE203775T1 (de) Methode zur detektion einer gezielten nukleinsäure
WO2005047468A8 (en) Improved methods for detecting and measuring specific nucleic acid sequences
DE60017089D1 (de) Verwendung von fluoreszierenden "molecular beacons" zum nachweis von methylierten nukleinsäuren
CA2145719A1 (en) Detection of Nucleic Acid Amplification
Takei et al. RT‐Hpro‐PCR: a MicroRNA detection system using a primer with a DNA tag
Roychoudhury et al. Influence of nucleotide sequence adjacent to duplex DNA termini on 3′ terminal labeling by terminal transferase
DE60119173D1 (de) Methode zur analyse von oligonukleotidanaloga
CN101871016A (zh) 基于环介导等温扩增技术的鸭肠炎病毒检测试剂盒及方法
ATE287969T1 (de) Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung
DE68926460D1 (de) Systeme für amplifikation/nachweis von nukleinsäuren
Livache et al. Detection of HIV1 DNA in biological samples by an homogeneous assay: fluorescence measurement of double-stranded RNA synthesized from amplified DNA
Xu et al. Detecting 5-methylcytosine using an enzyme-free DNA strand exchange reaction without pretreatment under physiological conditions

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 857218

Country of ref document: ES