ES2185719T3 - Deteccion de acidos nucleicos por formacion de un producto catalizada por diana. - Google Patents

Deteccion de acidos nucleicos por formacion de un producto catalizada por diana.

Info

Publication number
ES2185719T3
ES2185719T3 ES95944648T ES95944648T ES2185719T3 ES 2185719 T3 ES2185719 T3 ES 2185719T3 ES 95944648 T ES95944648 T ES 95944648T ES 95944648 T ES95944648 T ES 95944648T ES 2185719 T3 ES2185719 T3 ES 2185719T3
Authority
ES
Spain
Prior art keywords
analyte
polynucleotidic
fragment
detection
oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95944648T
Other languages
English (en)
Inventor
Edwin F Ullman
Linda M Western
Samuel J Rose
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dade Behring Inc
Original Assignee
Dade Behring Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23429108&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2185719(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dade Behring Inc filed Critical Dade Behring Inc
Application granted granted Critical
Publication of ES2185719T3 publication Critical patent/ES2185719T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SE DESCRIBE UN PROCEDIMIENTO PARA MODIFICAR UN OLIGONUCLEOTIDO, TENIENDO DICHO PROCEDIMIENTO UNA APLICACION EN LA DETECCION DE UN ANALITO POLINUCLEOTIDICO. UN OLIGONUCLEOTIDO SE HIBRIDA DE FORMA REVERSIBLE CON UN POLINUCLEOTIDO, POR EJEMPLO, UN ANALITO POLINUCLEOTIDICO, EN PRESENCIA DE UNA 5 CONDICIONES ISOTERMAS. EL ANALITO POLINUCLEOTIDICO ACTUA COMO ELEMENTO DE RECONOCIMIENTO PARA PERMITIR QUE UNA 5 ESCINDA EL OLIGONUCLEOTIDO PARA SUMINISTRAR (I) UN PRIMER FRAGMENTO QUE ES SUSTANCIALMENTE NO HIBRIDABLE CON EL ANALITO POLINUCLEOTIDICO, Y (II) UN SEGUNDO FRAGMENTO QUE SE ENCUENTRA 3 CON RESPECTO AL PRIMER FRAGMENTO (EN EL OLIGONUCLEOTIDO INTACTO) Y QUE ES SUSTANCIALMENTE HIBRIDABLE CON EL ANALITO POLINUCLEOTIDICO. SE OBTIENE AL MENOS UNA EXCESO MOLAR EN 100 VECES DEL PRIMER FRAGMENTO Y/O EL SEGUNDO FRAGMENTO EN RELACION A LA CANTIDAD MOLAR DEL ANALITO POLINUCLEOTIDICO. SE DETECTA LA PRESENCIA DEL PRIMER FRAGMENTO Y/O DEL SEGUNDO FRAGMENTO, INDICANDO SU PRESENCIA LA PRESENCIA DEL ANALITO POLINUCLEOTIDICO. EL PROCEDIMIENTO ENCUENTRA UNA APLICACION PARTICULAR EN LA DETECCION DE UN ANALITO POLINUCLEOTIDICO, TAL COMO EL ADN. TAMBIEN SE DESCRIBEN ESTUCHES DE ENSAYO PARA LLEVAR A CABO LOS PROCEDIMIENTOS SEGUN LA PRESENTE INVENCION.
ES95944648T 1994-12-23 1995-12-22 Deteccion de acidos nucleicos por formacion de un producto catalizada por diana. Expired - Lifetime ES2185719T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36316994A 1994-12-23 1994-12-23

Publications (1)

Publication Number Publication Date
ES2185719T3 true ES2185719T3 (es) 2003-05-01

Family

ID=23429108

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95944648T Expired - Lifetime ES2185719T3 (es) 1994-12-23 1995-12-22 Deteccion de acidos nucleicos por formacion de un producto catalizada por diana.

Country Status (5)

Country Link
US (7) US5792614A (es)
EP (2) EP0842294B1 (es)
DE (1) DE69528670T2 (es)
ES (1) ES2185719T3 (es)
WO (1) WO1996020287A2 (es)

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WO1996020287A3 (en) 1996-09-06
US20050191676A1 (en) 2005-09-01
US6121001A (en) 2000-09-19
US6368803B1 (en) 2002-04-09
US6110677A (en) 2000-08-29
US5792614A (en) 1998-08-11
US20080213767A1 (en) 2008-09-04
EP1260593A2 (en) 2002-11-27
DE69528670D1 (de) 2002-11-28
EP0842294B1 (en) 2002-10-23
US20020155548A1 (en) 2002-10-24
EP0842294A2 (en) 1998-05-20
EP1260593A3 (en) 2003-12-03
WO1996020287A2 (en) 1996-07-04
DE69528670T2 (de) 2004-03-11

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