ES2181900T3 - Clonacion recombinante in vitro usanto sitios de recombinacion por ingenieria genetica. - Google Patents

Clonacion recombinante in vitro usanto sitios de recombinacion por ingenieria genetica.

Info

Publication number
ES2181900T3
ES2181900T3 ES96923288T ES96923288T ES2181900T3 ES 2181900 T3 ES2181900 T3 ES 2181900T3 ES 96923288 T ES96923288 T ES 96923288T ES 96923288 T ES96923288 T ES 96923288T ES 2181900 T3 ES2181900 T3 ES 2181900T3
Authority
ES
Spain
Prior art keywords
isolated
nucleic acid
mutation
recombination
usanto
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96923288T
Other languages
English (en)
Inventor
James L Hartley
Michael A Brasch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ES2181900T3 publication Critical patent/ES2181900T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

SE PUEDE CONSEGUIR UNA CLONACION RECOMBINANTE MEDIANTE EL USO DE ACIDOS NUCLEICOS, VECTORES Y PROCEDIMIENTOS, IN VITRO E IN VIVO, PARA MOVER O INTERCAMBIAR SEGMENTOS DE MOLECULAS DE ADN UTILIZANDO PUNTOS DE RECOMBINACION MODIFICADOS POR INGENIERIA Y PROTEINAS DE RECOMBINACION PARA OBTENER MOLECULAS QUIMERICAS DE ADN CON UNA(S) CARACTERISTICA(S) Y/O SEGMENTO(S) DE ADN PARTICULARES.
ES96923288T 1995-06-07 1996-06-07 Clonacion recombinante in vitro usanto sitios de recombinacion por ingenieria genetica. Expired - Lifetime ES2181900T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US48613995A 1995-06-07 1995-06-07

Publications (1)

Publication Number Publication Date
ES2181900T3 true ES2181900T3 (es) 2003-03-01

Family

ID=23930754

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96923288T Expired - Lifetime ES2181900T3 (es) 1995-06-07 1996-06-07 Clonacion recombinante in vitro usanto sitios de recombinacion por ingenieria genetica.

Country Status (12)

Country Link
US (4) US5888732A (es)
EP (4) EP1229113A3 (es)
JP (6) JP4020429B2 (es)
AT (1) ATE222289T1 (es)
AU (1) AU724922B2 (es)
CA (1) CA2226463A1 (es)
DE (1) DE69623057T2 (es)
DK (1) DK0937098T3 (es)
ES (1) ES2181900T3 (es)
NZ (2) NZ500843A (es)
PT (1) PT937098E (es)
WO (1) WO1996040724A1 (es)

Families Citing this family (241)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE1006085A3 (fr) * 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
US7176029B2 (en) * 1992-07-31 2007-02-13 Universite Libre De Bruxelles Cloning and/or sequencing vector
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6964861B1 (en) * 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
CA2226463A1 (en) 1995-06-07 1996-12-19 Life Technologies Corporation Recombinational cloning using engineered recombination sites
US6534314B1 (en) 1996-06-14 2003-03-18 Massachusetts Institute Of Technology Methods and compositions for transforming cells
WO1998030684A1 (en) 1997-01-08 1998-07-16 Life Technologies, Inc. Methods for production of proteins
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US6140129A (en) * 1997-09-17 2000-10-31 Wisconsin Alumni Research Foundation Chromosomal targeting in bacteria using FLP recombinase
SE9703663D0 (sv) * 1997-10-08 1997-10-08 Pharmacia & Upjohn Ab Beta recombinase
US6780644B1 (en) 1997-10-08 2004-08-24 Pharmacia Spain S.A. Use of beta recombinase in eukaryotic cells, especially for transgenic work
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
US7393632B2 (en) * 1999-12-10 2008-07-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
DE69836092T2 (de) * 1997-10-24 2007-05-10 Invitrogen Corp., Carlsbad Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
US6262341B1 (en) 1997-11-18 2001-07-17 Pioneer Hi-Bred International, Inc. Method for the integration of foreign DNA into eukaryotic genomes
ES2245487T3 (es) * 1997-11-18 2006-01-01 Pioneer Hi-Bred International, Inc. Composiciones y metodos para la modificacion genetica de plantas.
ATE454459T1 (de) * 1997-11-18 2010-01-15 Pioneer Hi Bred Int Mobilisierung eines viralen genoms aus t-dna durch ortsspezifische rekombinationssysteme
US7102055B1 (en) 1997-11-18 2006-09-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant
CA2327387C (en) * 1998-05-07 2012-07-03 Gx Biosystems A/S Cytotoxin-based biological containment
US6480510B1 (en) 1998-07-28 2002-11-12 Serconet Ltd. Local area network of serial intelligent cells
WO2000011155A1 (en) 1998-08-19 2000-03-02 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for genomic modification
WO2000012687A1 (en) * 1998-08-28 2000-03-09 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
EP1131078A4 (en) * 1998-11-13 2005-08-24 Invitrogen Corp COMPOSITIONS AND METHODS FOR RECOMBINATORY CLONING OF NUCLEIC ACID MOLECULES
DE60042969D1 (de) * 1999-03-02 2009-10-29 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
US6890726B1 (en) 1999-04-06 2005-05-10 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
JP2003501082A (ja) 1999-06-07 2003-01-14 セル ジェネシス インコーポレイテッド ハイブリッド酵母−細菌クローニング系およびその使用
US6709852B1 (en) * 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
JP2003510019A (ja) * 1999-07-14 2003-03-18 クロンテック・ラボラトリーズ・インコーポレーテッド リコンビナーゼを利用した発現ベクターを作製する方法、およびこの方法を実施するために使用する組成物
US6746870B1 (en) * 1999-07-23 2004-06-08 The Regents Of The University Of California DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
DE19941186A1 (de) * 1999-08-30 2001-03-01 Peter Droege Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
FR2799472B1 (fr) * 1999-10-07 2004-07-16 Aventis Pharma Sa Preparation d'adenovirus recombinants et de banques adenovirales
CA2388907A1 (en) * 1999-10-25 2001-05-03 Life Technologies Corporation Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination
DE19952983A1 (de) * 1999-11-03 2001-05-17 Acgt Progenomics Ag Verfahren zum Transfer von molekularen Substanzen mit prokaryontischen nukleinsäurebindenden Proteinen
EP1111061A1 (en) * 1999-12-20 2001-06-27 Universite Libre De Bruxelles Double selection vector
US6808925B2 (en) * 2000-02-18 2004-10-26 The Board Of Trustees Of The Leland Stanford Junior University Altered recombinases for genome modification
US20040146918A1 (en) * 2000-02-18 2004-07-29 Weiner Michael L. Hybrid nucleic acid assembly
US7517688B2 (en) * 2000-02-25 2009-04-14 Stratagene California Method for ligating nucleic acids and molecular cloning
WO2001062892A2 (en) * 2000-02-25 2001-08-30 Stratagene Method for ligating nucleic acids and molecular cloning
US6842459B1 (en) * 2000-04-19 2005-01-11 Serconet Ltd. Network combining wired and non-wired segments
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6551828B1 (en) 2000-06-28 2003-04-22 Protemation, Inc. Compositions and methods for generating expression vectors through site-specific recombination
US7087415B2 (en) * 2000-07-31 2006-08-08 Athena Biotechnologies, Inc. Methods and compositions for directed gene assembly
CA2419322C (en) 2000-08-14 2012-10-23 Donald L. Court Enhanced homologous recombination mediated by lambda recombination proteins
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7560622B2 (en) * 2000-10-06 2009-07-14 Pioneer Hi-Bred International, Inc. Methods and compositions relating to the generation of partially transgenic organisms
WO2002103013A2 (en) * 2000-10-30 2002-12-27 Gene Logic, Inc. Partially double-stranded nucleic acids, methods of making, and use thereof
JP2004513638A (ja) * 2000-11-10 2004-05-13 アストラゼネカ・アクチエボラーグ ベクター
GB0027501D0 (en) * 2000-11-10 2000-12-27 Astrazeneca Ab Vector
AU2002227153B2 (en) * 2000-12-08 2008-01-24 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7214515B2 (en) * 2001-01-05 2007-05-08 The General Hospital Corporation Viral delivery system for infectious transfer of large genomic DNA inserts
US6977165B2 (en) 2001-01-18 2005-12-20 Clontech Laboratories, Inc. Sequence specific recombinase-based methods for producing intron containing vectors and compositions for use in practicing the same
ATE538380T1 (de) * 2001-01-23 2012-01-15 Harvard College Nukleinsäure-programmierbare protein-arrays
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
CA2429397C (en) 2001-01-26 2014-06-03 Commonwealth Scientific And Industrial Research Organisation Methods and means for producing efficient silencing construct using recombinational cloning
US8470580B2 (en) * 2001-02-23 2013-06-25 Universite Libre De Bruxelles Method for the selection of recombination clones comprising a sequence encoding an antidote protein to a toxic molecule
US6696278B1 (en) * 2001-02-26 2004-02-24 Stratagene Method for transfer of DNA segments
US20050090010A1 (en) * 2001-03-02 2005-04-28 Yoshihide Hayashizaki Cloning vectors and method for molecular cloning
US20030165873A1 (en) 2001-03-02 2003-09-04 Come Jon H. Three hybrid assay system
US20030170642A1 (en) * 2001-03-12 2003-09-11 Irm, Llc Identification of cellular targets for biologically active molecules
WO2002072789A2 (en) * 2001-03-12 2002-09-19 Irm, Llc. Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
WO2002081711A1 (en) * 2001-04-06 2002-10-17 Cropdesign N.V. The use of double and opposite recombination sites for the single step cloning of two dna segments
JP2004531259A (ja) * 2001-04-19 2004-10-14 インヴィトロジェン コーポレーション 核酸分子の組換えクローニングのための組成物および方法
CA2448505A1 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US20050287647A9 (en) * 2001-05-30 2005-12-29 Carl Perez Plant artificial chromosomes, uses thereof and methods of preparing plant artificial chromosomes
JP4489424B2 (ja) * 2001-05-30 2010-06-23 グラクソ グループ リミテッド 染色体に基くプラットホーム
US20030143612A1 (en) * 2001-07-18 2003-07-31 Pointilliste, Inc. Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
CN1312288C (zh) * 2001-08-28 2007-04-25 华中农业大学 一种位点重组反向克隆基因方法及其应用
WO2003025161A1 (en) 2001-09-18 2003-03-27 Clontech Laboratories, Inc. Site-specific recombinase based method for producing adenoviral vectors
WO2003029456A1 (en) 2001-10-01 2003-04-10 Dyax Corp. Multi-chain eukaryotic display vectors and uses thereof
US7026460B2 (en) * 2001-10-09 2006-04-11 Microbia, Inc. lovE variant regulator molecules
US7196189B2 (en) * 2001-10-09 2007-03-27 Microbia, Inc. love variant regulator molecules
US20050118690A1 (en) * 2001-10-09 2005-06-02 Shannon Roberts Love variant regulator molecules
WO2003038049A2 (en) * 2001-10-29 2003-05-08 Renovis, Inc. Method for isolating cell-type specific mrnas
AU2002366039A1 (en) 2001-11-22 2003-06-10 Keio University Artificial antibody library with super-repertory
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
US7338800B2 (en) * 2002-01-16 2008-03-04 Baylor College Of Medicine In vivo gene transfer
US6594086B1 (en) * 2002-01-16 2003-07-15 Optonics, Inc. (A Credence Company) Bi-convex solid immersion lens
WO2003062402A2 (en) * 2002-01-24 2003-07-31 Pointilliste, Inc. Use of collections of binding sites for sample profiling and other applications
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
WO2003078638A1 (en) * 2002-03-19 2003-09-25 Universite Libre De Bruxelles Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms
US20040038304A1 (en) * 2002-03-28 2004-02-26 Gala Design, Inc. Antibody libraries
CA2480642C (en) * 2002-03-29 2012-10-16 Janet Louise Suttie Lambda integrase mediated recombination in plants
CN1863915B (zh) * 2002-03-29 2011-02-02 辛根塔参与股份公司 植物中λ整合酶介导的重组
US20080076678A1 (en) 2005-06-15 2008-03-27 Philippe Soucaille Method of creating a library of bacterial clones with varying levels of gene expression
ATE526419T1 (de) * 2002-04-22 2011-10-15 Danisco Us Inc Verfahren zur erzeugung zu variierenden genexpressionsniveaus führender modifizierter promotoren
JP2005523015A (ja) * 2002-04-22 2005-08-04 ジェネンコー・インターナショナル・インク 種々の遺伝子発現レベルを有する細菌クローンのライブラリーを構築する方法。
US8304233B2 (en) 2002-06-04 2012-11-06 Poetic Genetics, Llc Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same
EP1539998A4 (en) * 2002-06-05 2007-10-31 Invitrogen Corp METHODS AND COMPOSITIONS FOR SYNTHESIS OF NUCLEIC ACID MOLECULES USING MULTIPLE RECOGNITION SITES
DE60319259T2 (de) * 2002-06-14 2009-03-05 Dyax Corp., Cambridge Rekombination von nukleinsäurebibliotheksmitgliedern
US20040002155A1 (en) * 2002-06-26 2004-01-01 Stratagene Endonuclease VII cloning method
US20040172667A1 (en) * 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
US20040002156A1 (en) * 2002-06-26 2004-01-01 Stratagene Selective cloning of homoduplex nucleic acids
US7527966B2 (en) * 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US20040235011A1 (en) * 2002-06-26 2004-11-25 Cooper Richard K. Production of multimeric proteins
US20040132133A1 (en) * 2002-07-08 2004-07-08 Invitrogen Corporation Methods and compositions for the production, identification and purification of fusion proteins
AU2003253992A1 (en) * 2002-07-18 2004-02-09 Robert P. Bennett Viral vectors containing recombination sites
AU2003257109A1 (en) * 2002-08-05 2004-02-23 Invitrogen Corporation Compositions and methods for molecular biology
US9309518B2 (en) 2002-09-03 2016-04-12 Universite Libre De Bruxelles Reversible, parallel and multitask cloning method and kit
CN1703509B (zh) * 2002-09-03 2010-09-01 布鲁塞尔自由大学 可逆的、平行的和多任务的克隆方法和试剂盒
AU2003270619A1 (en) 2002-09-12 2004-04-30 Molecular Probes, Inc. Site-specific labeling of affinity tags in fusion proteins
CN1263860C (zh) * 2002-09-30 2006-07-12 华南农业大学 多基因载体的构建方法与应用
WO2004042019A2 (en) * 2002-10-30 2004-05-21 Pointilliste, Inc. Systems for capture and analysis of biological particles and methods using the systems
US7267984B2 (en) * 2002-10-31 2007-09-11 Rice University Recombination assembly of large DNA fragments
WO2004044151A2 (en) * 2002-11-07 2004-05-27 University Of Rochester Recombinase mediated transcription
US20040115642A1 (en) * 2002-12-12 2004-06-17 Changlin Fu Single-step methods for gene cloning
US7078234B2 (en) 2002-12-18 2006-07-18 Monsanto Technology Llc Maize embryo-specific promoter compositions and methods for use thereof
US20040209370A1 (en) * 2002-12-19 2004-10-21 Wonchul Suh Method for chromosomal engineering
CA2513072A1 (en) * 2003-01-09 2004-07-29 Invitrogen Corporation Cellular delivery and activation polypeptide-nucleic acid complexes
US20040214306A1 (en) * 2003-01-23 2004-10-28 Bloom Fredric R. Rapid growing microorganisms for biotechnology applications
US20040241748A1 (en) * 2003-02-10 2004-12-02 Dana Ault-Riche Self-assembling arrays and uses thereof
EP1881074B1 (en) 2003-05-05 2013-01-02 Monsanto Technology, LLC HRGP promoter constructs
US7521242B2 (en) * 2003-05-09 2009-04-21 The United States Of America As Represented By The Department Of Health And Human Services Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
WO2004104194A1 (en) * 2003-05-15 2004-12-02 Invitrogen Corporation Compositions and methods for site-specific recombination and expression
US20050095615A1 (en) * 2003-06-26 2005-05-05 Welch Peter J. Methods and compositions for detecting promoter activity and expressing fusion proteins
US20060286600A1 (en) * 2003-06-30 2006-12-21 Albertha Walhout High throughput one-hybrid system
US20070093605A1 (en) * 2003-07-30 2007-04-26 Adur Ashok M Nucleated thermoplastic elastomer compositions and related methods
WO2005014796A2 (en) 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
EP2272967B1 (en) 2003-10-02 2015-12-23 Monsanto Technology LLC Stacking crop improvement traits in transgenic plants
WO2005035769A2 (en) 2003-10-09 2005-04-21 E. I. Du Pont De Nemours And Company Gene silencing by using micro-rna molecules
US20050095648A1 (en) * 2003-10-30 2005-05-05 Mario Geysen Method for designing linear epitopes and algorithm therefor and polypeptide epitopes
JP2007512838A (ja) 2003-12-01 2007-05-24 インヴィトロジェン コーポレーション 組換え部位を含む核酸分子およびその使用方法
US7935862B2 (en) * 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
WO2005062881A2 (en) 2003-12-24 2005-07-14 Transgenrx, Inc. Gene therapy using transposon-based vectors
US20060018911A1 (en) * 2004-01-12 2006-01-26 Dana Ault-Riche Design of therapeutics and therapeutics
US20050227309A1 (en) 2004-01-21 2005-10-13 Corry Schuyler B Optically-detectable enzyme substrates and their method of use
AR047598A1 (es) 2004-02-10 2006-01-25 Monsanto Technology Llc Semilla de maiz transgenica con mayor contenido de aminoacidos
US20090098611A1 (en) * 2004-02-27 2009-04-16 Wood David W Self-cleaving affinity tags and methods of use
US20050260653A1 (en) * 2004-04-14 2005-11-24 Joshua Labaer Nucleic-acid programmable protein arrays
US20050282265A1 (en) * 2004-04-19 2005-12-22 Laura Vozza-Brown Electroporation apparatus and methods
US7674621B2 (en) 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
AU2005268917B2 (en) 2004-08-02 2010-05-20 Basf Plant Science Gmbh Method for isolation of transcription termination sequences
WO2006060595A2 (en) * 2004-12-01 2006-06-08 Invitrogen Corporation Reverse two hybrid system for identification of interaction domains
WO2006073727A2 (en) 2004-12-21 2006-07-13 Monsanto Technology, Llc Recombinant dna constructs and methods for controlling gene expression
US20060200878A1 (en) 2004-12-21 2006-09-07 Linda Lutfiyya Recombinant DNA constructs and methods for controlling gene expression
US8314290B2 (en) 2004-12-21 2012-11-20 Monsanto Technology Llc Temporal regulation of gene expression by MicroRNAs
CA2591992A1 (en) 2004-12-22 2006-06-29 The Salk Institute For Biological Studies Compositions and methods for producing recombinant proteins
US7582475B2 (en) 2005-01-07 2009-09-01 University Of Georgia Research Foundation, Inc. Vectors and methods for high throughput co-expression
CA2606220A1 (en) * 2005-04-19 2006-12-21 Basf Plant Science Gmbh Starchy-endosperm and/or germinating embryo-specific expression in mono-cotyledonous plants
USD564100S1 (en) 2005-04-19 2008-03-11 Invitrogen Corporation Electroporation vessel
ATE545701T1 (de) * 2005-05-17 2012-03-15 Ozgene Pty Ltd Sequentielles klonierungssystem
US9121028B2 (en) * 2005-09-09 2015-09-01 Monsanto Technology Llc Selective gene expression in plants
US20070117179A1 (en) * 2005-09-27 2007-05-24 Invitrogen Corporation In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles
US20070243617A1 (en) * 2005-10-13 2007-10-18 Holt Robert A Modular genomes for synthetic biology and metabolic engineering
CA2625031C (en) 2005-10-13 2016-07-19 Monsanto Technology Llc Methods for producing hybrid seed
WO2007100384A2 (en) * 2005-10-24 2007-09-07 Invitrogen Corporation Three frame cdna expression libraries for functional screening of proteins
US20070231906A1 (en) * 2005-10-25 2007-10-04 Invitrogen Corporation Three Frame cDNA Expression Libraries for Functional Screening of Proteins
US20070143881A1 (en) * 2005-12-16 2007-06-21 Pioneer Hi-Bred International, Inc. Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
CN101490267B (zh) 2006-05-17 2013-04-17 先锋高级育种国际公司 人工植物微染色体
WO2007137300A2 (en) 2006-05-23 2007-11-29 Bellicum Pharmaceuticals, Inc. Modified dendritic cells having enhanced survival and immunogenicity and related compositions and methods
US8178316B2 (en) * 2006-06-29 2012-05-15 President And Fellows Of Harvard College Evaluating proteins
EP2059596B1 (en) 2006-08-31 2017-10-04 Monsanto Technology, LLC Phased small rnas
EP2664673A3 (en) 2006-10-05 2014-02-19 E. I. du Pont de Nemours and Company Maize microRNA sequences
CN101802215A (zh) 2006-10-12 2010-08-11 孟山都技术有限公司 植物微rna及其使用方法
WO2008095927A1 (en) * 2007-02-05 2008-08-14 Philipps-Universität Marburg Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
CA2675926A1 (en) 2007-02-16 2008-08-21 Basf Plant Science Gmbh Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants
WO2008103643A1 (en) 2007-02-20 2008-08-28 Monsanto Technology, Llc Invertebrate micrornas
EP2118129A4 (en) * 2007-03-01 2010-04-28 Life Technologies Corp ISOLATED PHOSPHOLIPID PROTEIN PARTICLES
EP1997898A1 (en) * 2007-06-01 2008-12-03 Rijksuniversiteit Groningen Means and methods for cloning nucleic acid sequences
US8912392B2 (en) 2007-06-29 2014-12-16 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
WO2009068645A1 (en) * 2007-11-30 2009-06-04 Boehringer Ingelheim Pharma Gmbh & Co. Kg Novel recombination sequences
JP5670755B2 (ja) 2008-03-12 2015-02-18 ザ ロックフェラー ユニバーシティ 翻訳プロファイリング及び分子表現型解析のための方法及び組成物
US20090280103A1 (en) * 2008-04-04 2009-11-12 Martin Flueck Regulation of muscle repair
US9040774B2 (en) 2008-07-01 2015-05-26 Monsanto Technology Llc Recombinant DNA constructs encoding ribonuclease cleavage blockers and methods for modulating expression of a target gene
US9150880B2 (en) * 2008-09-25 2015-10-06 Proteovec Holding, L.L.C. Vectors for production of antibodies
EP2342224A2 (en) * 2008-09-25 2011-07-13 TransGenRx, Inc. Novel vectors for production of interferon
WO2010036978A2 (en) * 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of growth hormone
CN102369288A (zh) 2008-11-14 2012-03-07 生命技术公司 用于工程化细胞的组合物和方法
US9150881B2 (en) * 2009-04-09 2015-10-06 Proteovec Holding, L.L.C. Production of proteins using transposon-based vectors
WO2011003034A2 (en) 2009-07-02 2011-01-06 Verdezyne, Inc. Biological methods for preparing adipic acid
AP3766A (en) 2009-07-09 2016-07-31 Du Pont Engineered microorganisms with enhanced fermentation activity
US8889394B2 (en) * 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
WO2011058555A1 (en) * 2009-11-12 2011-05-19 Yeda Research And Development Co. Ltd. A method of editing dna in a cell and constructs capable of same
US10100318B2 (en) 2009-11-13 2018-10-16 Massachusetts Institute Of Technology Bio-field programmable gate array and bio-programmable logic array: reconfigurable chassis construction
US20110150841A1 (en) 2009-12-16 2011-06-23 University Of Washington Secretion System and Methods for its Use
US8704041B2 (en) 2009-12-30 2014-04-22 Pioneer Hi Bred International Inc Methods and compositions for targeted polynucleotide modification
CA2793600C (en) 2009-12-30 2020-01-14 Pioneer Hi-Bred International, Inc. Methods and compositions for the introduction and regulated expression of genes in plants
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
EP2471909A1 (en) 2010-12-30 2012-07-04 SIRION BIOTECH GmbH Nucleic acid molecules for generating adenoviral vectors
WO2012129373A2 (en) 2011-03-23 2012-09-27 Pioneer Hi-Bred International, Inc. Methods for producing a complex transgenic trait locus
US8685737B2 (en) 2011-04-27 2014-04-01 Amyris, Inc. Methods for genomic modification
US8728798B2 (en) 2011-05-03 2014-05-20 Verdezyne, Inc. Biological methods for preparing adipic acid
US8343752B2 (en) 2011-05-03 2013-01-01 Verdezyne, Inc. Biological methods for preparing adipic acid
US9499837B2 (en) 2011-06-21 2016-11-22 E.I. Du Pont De Nemours And Company Methods and compositions for producing male sterile plants
CA2841796C (en) 2011-07-06 2021-06-29 Verdezyne, Inc. Biological methods for preparing a fatty dicarboxylic acid
EP3964285A1 (en) 2011-09-26 2022-03-09 Thermo Fisher Scientific Geneart GmbH High efficiency, small volume nucleic acid synthesis
EP2795501A2 (en) 2011-12-21 2014-10-29 Life Technologies Corporation Methods and systems for in silico experimental designing and performing a biological workflow
WO2013152220A2 (en) 2012-04-04 2013-10-10 Life Technologies Corporation Tal-effector assembly platform, customized services, kits and assays
US10358655B1 (en) 2012-06-27 2019-07-23 University Of Utah Research Foundation Attenuated protein expression vectors and associated methods
US20140173775A1 (en) 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic plants
WO2014100504A2 (en) 2012-12-19 2014-06-26 Verdezyne, Inc. Biological methods for preparing a fatty dicarboxylic acid
HK1216431A1 (zh) 2012-12-19 2016-11-11 科威生物制品有限公司 制备脂肪二羧酸的生物学方法
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN103215296B (zh) * 2013-03-22 2014-12-03 武汉伯远生物科技有限公司 一种多片段dna分子组装方法及应用
US10253321B2 (en) 2013-05-01 2019-04-09 Dna2.0, Inc. Methods, compositions and kits for a one-step DNA cloning system
US9206433B2 (en) * 2013-05-01 2015-12-08 Dna Twopointo, Inc. Methods, compositions and kits for a one-step DNA cloning system
CN110564663B (zh) 2013-08-19 2023-03-10 辛格隆股份公司 微生物的受控生长
WO2015095804A1 (en) 2013-12-19 2015-06-25 Amyris, Inc. Methods for genomic integration
KR102352024B1 (ko) 2014-03-11 2022-01-14 디씨지 시스템스 인코포레이티드 Sil 팁을 플로팅하는 것을 자체 정정
HUE056155T2 (hu) 2014-04-09 2022-01-28 Scripps Research Inst Nem-természetes vagy módosított nukleozid-trifoszfátok sejtekbe importálása nukleinsav-trifoszfát transzporterekkel
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica
US10407676B2 (en) 2014-12-09 2019-09-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20180148744A1 (en) 2015-03-20 2018-05-31 Verdezyne, Inc. Biological methods for preparing 3-hydroxypropionic acid
CN105063087A (zh) * 2015-07-30 2015-11-18 赛业（苏州）生物科技有限公司 一种基于rmce技术的转基因方法
WO2017106767A1 (en) 2015-12-18 2017-06-22 The Scripps Research Institute Production of unnatural nucleotides using a crispr/cas9 system
JP7093310B2 (ja) 2016-05-18 2022-06-29 アミリス， インコーポレイテッド 外因性ランディングパッドへの核酸のゲノム組込みのための組成物および方法
EP4163293A1 (en) 2016-06-24 2023-04-12 The Scripps Research Institute Novel nucleoside triphosphate transporter and uses thereof
SI3645719T1 (sl) 2017-06-30 2022-07-29 Inscripta, Inc., Postopki, moduli, instrumenti in sistemi za avtomatizirano obdelavo celic
CA3069321A1 (en) 2017-07-11 2019-01-17 The Scripps Research Institute Incorporation of unnatural nucleotides and methods thereof
US11174488B2 (en) 2017-07-13 2021-11-16 Radici Chimica S.P.A. Biological methods for modifying cellular carbon flux
KR20250145137A (ko) 2017-08-03 2025-10-13 신톡스, 인크. 자가면역 질환의 치료를 위한 사이토카인 접합체
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
AU2018338990B2 (en) 2017-09-30 2022-06-23 Inscripta, Inc. Flow through electroporation instrumentation
KR102777327B1 (ko) 2017-12-19 2025-03-05 신굴론 에스.에이. 발효 공정
CN112105627B (zh) 2017-12-29 2024-07-02 斯克利普斯研究所 非天然碱基对组合物及使用方法
EP3775159A4 (en) 2018-03-29 2022-01-19 Inscripta, Inc. AUTOMATED REGULATION OF CELL GROWTH RATES FOR INDUCTION AND TRANSFORMATION
US10376889B1 (en) 2018-04-13 2019-08-13 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
US10501738B2 (en) 2018-04-24 2019-12-10 Inscripta, Inc. Automated instrumentation for production of peptide libraries
AU2019292919A1 (en) 2018-06-30 2021-03-11 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
EP3833685A4 (en) 2018-07-08 2022-08-17 Specifica Inc. ANTIBODY LIBRARIES WITH FEATURES FOR MAXIMIZED ANTIBODY DEVELOPMENT
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
AU2019363487A1 (en) 2018-08-30 2021-04-15 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
WO2020060948A1 (en) 2018-09-17 2020-03-26 Levadura Biotechnology, Inc. Production of cannabinoids in yeast using a fatty acid feedstock
CN109371047B (zh) * 2018-09-30 2021-11-09 华侨大学 一种利用蛋白IHF-α构建和表达耐热性抗菌肽融合蛋白的方法
SG11202107354WA (en) 2019-02-06 2021-08-30 Synthorx Inc Il-2 conjugates and methods of use thereof
TWI874408B (zh) 2019-06-14 2025-03-01 美商史基普研究協會 於半合成生物體中複製、轉錄及轉譯之試劑及方法
CN114008070A (zh) 2019-06-21 2022-02-01 因思科瑞普特公司 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
KR20220155584A (ko) 2020-03-15 2022-11-23 프로티니아, 인크. 곤충에서의 재조합 단백질 생산
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
WO2023200770A1 (en) 2022-04-12 2023-10-19 Inscripta, Inc. Curing for iterative nucleic acid-guided nuclease editing

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4673640A (en) * 1984-04-30 1987-06-16 Biotechnica International, Inc. Regulated protein production using site-specific recombination
US4743546A (en) 1985-02-13 1988-05-10 Biotechnica International, Inc. Controlled gene excision
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
EP0300422B1 (en) * 1987-07-21 1992-12-02 The Du Pont Merck Pharmaceutical Company An improved method for preparing stable and viable recombinant animal cell viral vectors
CA2012983A1 (en) * 1989-03-27 1990-09-27 Sydney Brenner Process for nucleic acid detection by binary amplification
EP0489002B1 (en) 1989-08-22 1994-12-28 E.I. Du Pont De Nemours And Company An in vitro packaging system for cloning dna fragments of 95 kb
NO904633L (no) * 1989-11-09 1991-05-10 Molecular Diagnostics Inc Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe.
US5658772A (en) 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
EP0528881A4 (en) * 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
EP0585287B1 (en) 1990-07-10 1999-10-13 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US5227288A (en) * 1990-10-01 1993-07-13 Blattner Frederick R DNA sequencing vector with reversible insert
US5286632A (en) 1991-01-09 1994-02-15 Jones Douglas H Method for in vivo recombination and mutagenesis
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
WO1992018521A1 (en) 1991-04-10 1992-10-29 Life Technologies, Inc. Method for amplifying and altering an rna sequence
US5858657A (en) 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
US5871907A (en) 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
WO1993010086A1 (de) 1991-11-13 1993-05-27 MTA Központi Kémiai Kutató Intézete Verfahren zur herstellung von n-(arylsulfonyl)-carbamid-säure-derivaten und den in diesem verfahren verwendbaren intermedieren
EP0626999A4 (en) * 1992-01-24 1997-04-09 Life Technologies Inc Modulation of enzyme activities in the -i(in vivo) cloning of dna.
ES2157217T3 (es) 1992-02-26 2001-08-16 Zeneca Mogen B V Cepas de agrobacterium capaces de recombinacion especifica para el sitio.
JP3507073B2 (ja) * 1992-03-24 2004-03-15 ケンブリッジ アンティボディー テクノロジー リミティド 特異的結合対の成員の製造方法
US5733743A (en) 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
BE1006085A3 (fr) 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5733733A (en) 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
WO1994009127A2 (en) * 1992-10-16 1994-04-28 The Government Of The United States Of America As Represented By The Secretary, Department Of Healthand Human Services Supercoiled minicircle dna as as a unitary promoter vector
JP3720353B2 (ja) 1992-12-04 2005-11-24 メディカル リサーチ カウンシル 多価および多重特異性の結合タンパク質、それらの製造および使用
US5334575A (en) 1992-12-17 1994-08-02 Eastman Kodak Company Dye-containing beads for laser-induced thermal dye transfer
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
US5919676A (en) 1993-06-24 1999-07-06 Advec, Inc. Adenoviral vector system comprising Cre-loxP recombination
EP0632054A1 (en) * 1993-06-28 1995-01-04 European Molecular Biology Laboratory Regulation of site-specific recombination by site-specific recombinase/nuclear receptor fusion proteins
US5843744A (en) * 1993-07-08 1998-12-01 Ecogen Inc. Bacillus thuringiensis Tn5401 proteins
US5441884A (en) 1993-07-08 1995-08-15 Ecogen Inc. Bacillus thuringiensis transposon TN5401
US5470727A (en) 1993-12-21 1995-11-28 Celtrix Pharmaceuticals, Inc. Chromosomal expression of non-bacterial genes in bacterial cells
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
WO1995032225A1 (en) 1994-05-23 1995-11-30 The Salk Institute For Biological Studies Method for site-specific integration of nucleic acids and related products
US5723765A (en) * 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
CA2226463A1 (en) 1995-06-07 1996-12-19 Life Technologies Corporation Recombinational cloning using engineered recombination sites
WO1997006265A2 (en) 1995-08-07 1997-02-20 The Perkin-Elmer Corporation Recombinant clone selection system
US5801030A (en) 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
AUPN523995A0 (en) 1995-09-05 1995-09-28 Crc For Biopharmaceutical Research Pty Ltd Method for producing phage display vectors
CA2239366A1 (en) 1996-01-05 1997-07-17 Genetic Therapy, Inc. Recombinase-mediated generation of adenoviral vectors
AU2319497A (en) 1996-03-07 1997-09-22 Regents Of The University Of California, The Helper-free, totally defective adenovirus for gene therapy
US5928914A (en) 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US5710248A (en) 1996-07-29 1998-01-20 University Of Iowa Research Foundation Peptide tag for immunodetection and immunopurification
IL128736A0 (en) 1996-09-06 2000-01-31 Univ Pennsylvania Methods using cre-lox for production of recombinant adeno-associated viruses
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
DE19720839B4 (de) 1997-05-17 2007-08-16 Dirk Dr. Schindelhauer Baukastenprinzip-Technologie zur Herstellung von langen, exakten DNA-Konstrukten in vitro, insbesondere für die Herstellung von Konstrukten zur Erzeugung von künstlichen Chromosomen (MAC, mammalian artificial chromosome) aus zwei stabil klonierten DNA-Komponenten und der ditelomerische Vektor-Prototyp PTAT
US5989872A (en) 1997-08-12 1999-11-23 Clontech Laboratories, Inc. Methods and compositions for transferring DNA sequence information among vectors
US7135608B1 (en) 1997-08-28 2006-11-14 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor
DE69836092T2 (de) 1997-10-24 2007-05-10 Invitrogen Corp., Carlsbad Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
JP4206154B2 (ja) 1997-11-13 2009-01-07 大日本住友製薬株式会社 変異型ｌｏｘＰ配列とその応用
US5874259A (en) 1997-11-21 1999-02-23 Wisconsin Alumni Research Foundation Conditionally amplifiable BAC vector
WO1999055851A2 (en) 1998-04-28 1999-11-04 Novartis Ag Site-directed transformation of plants
WO2000012687A1 (en) 1998-08-28 2000-03-09 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
EP1169458A2 (en) 1999-04-06 2002-01-09 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
JP2003510019A (ja) 1999-07-14 2003-03-18 クロンテック・ラボラトリーズ・インコーポレーテッド リコンビナーゼを利用した発現ベクターを作製する方法、およびこの方法を実施するために使用する組成物
WO2001011058A1 (en) 1999-08-09 2001-02-15 Monsanto Technology Llc Novel cloning methods and vectors

Also Published As

Publication number Publication date
EP0937098A4 (en) 1999-10-13
US6270969B1 (en) 2001-08-07
EP1227147A2 (en) 2002-07-31
PT937098E (pt) 2002-12-31
DK0937098T3 (da) 2002-12-02
JP2006087445A (ja) 2006-04-06
AU724922B2 (en) 2000-10-05
JP2009100776A (ja) 2009-05-14
EP1229113A3 (en) 2002-11-27
JP2003299487A (ja) 2003-10-21
JPH11507236A (ja) 1999-06-29
HK1019068A1 (en) 2000-01-21
EP0937098A1 (en) 1999-08-25
ATE222289T1 (de) 2002-08-15
JP4020429B2 (ja) 2007-12-12
JP2007306931A (ja) 2007-11-29
EP1229113A2 (en) 2002-08-07
NZ312332A (en) 2000-01-28
EP2322614A1 (en) 2011-05-18
EP0937098B1 (en) 2002-08-14
US6171861B1 (en) 2001-01-09
US5888732A (en) 1999-03-30
NZ500843A (en) 2002-03-28
US20100267118A1 (en) 2010-10-21
DE69623057T2 (de) 2003-03-27
JP2007020583A (ja) 2007-02-01
EP1227147A3 (en) 2002-08-14
CA2226463A1 (en) 1996-12-19
DE69623057D1 (de) 2002-09-19
AU6384196A (en) 1996-12-30
WO1996040724A1 (en) 1996-12-19

Similar Documents

Publication Publication Date Title
ES2181900T3 (es) Clonacion recombinante in vitro usanto sitios de recombinacion por ingenieria genetica.
Benne RNA editing in trypanosomes
DE69833457D1 (de) Neuartige methode zur integration von fremd-dna ins eukaryotische genom
CA2320100A1 (en) High affinity nucleic acid ligands of complement system proteins
DE60042969D1 (de) Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
JPH06510431A (ja) ジェロニン・ポリペプチドをコードするｄｎａ配列
CN102787125A (zh) 一种构建tale重复序列的方法
WO1996015144A3 (en) Chromosome 21 gene marker, compositions and methods using same
WO2023125814A1 (zh) 腺嘌呤脱氨酶及其应用
CA2461443A1 (en) Nucleic acid and amino acid sequences encoding high-level expressor factor viii polypeptides and methods of use
SI9500270B (sl) Rekombinantni mersacidin in postopek za izdelavo
Grant et al. Sequence-specific alkylation and cleavage of DNA mediated by purine motif triple helix formation
AU5120796A (en) DNA fragment, recombination vector containing the same and method for expressing foreign genes using the same
KR102151064B1 (ko) 매칭된 5' 뉴클레오타이드를 포함하는 가이드 rna를 포함하는 유전자 교정용 조성물 및 이를 이용한 유전자 교정 방법
Altenbuchner et al. Transposon Tn 1721: site-specific recombination generates deletions and inversions
JP2024509048A (ja) Ｃｒｉｓｐｒ関連トランスポゾンシステム及びその使用方法
JP2024509047A (ja) Ｃｒｉｓｐｒ関連トランスポゾンシステム及びその使用方法
CA2429451A1 (en) Isolated luciferases and their use
AU8447098A (en) Endogenetic retroviral sequences, associated with autoimmune diseases or with pregnancy disorders
Whitehouse Crossing-over
CN120092083A (zh) 包含Cas蛋白和细菌毒素的融合蛋白及其用途
KR102679001B1 (ko) 신규의 개량된 염기 편집 또는 교정용 융합단백질 및 이의 용도
DK0857220T3 (da) Sekvensmodifikation af oligonucleotidprimere til manipulering af ikke-templateret nucleotidaddition
SE9801098D0 (sv) Receptors
ATE259421T1 (de) Dna-virus-vektoren und verfahren zu ihrer herstellung