ES2552226T3 - Reparación y regeneración de cartílago y hueso utilizando células derivadas posparto - Google Patents

Reparación y regeneración de cartílago y hueso utilizando células derivadas posparto Download PDF

Info

Publication number
ES2552226T3
ES2552226T3 ES10183911.6T ES10183911T ES2552226T3 ES 2552226 T3 ES2552226 T3 ES 2552226T3 ES 10183911 T ES10183911 T ES 10183911T ES 2552226 T3 ES2552226 T3 ES 2552226T3
Authority
ES
Spain
Prior art keywords
cells
mitomycin
bone
treated
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES10183911.6T
Other languages
English (en)
Inventor
Anthony J. Kihm
Agnieszka Seyda
Sridevi Dhanaraj
Ziwei Wang
Alexander M. Harmon
Ian Ross Harris
Darin J. Messina
Sanjay Mistry
Chin-Feng Yi
Anna Gosiewska
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DePuy Synthes Products Inc
Original Assignee
DePuy Synthes Products Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DePuy Synthes Products Inc filed Critical DePuy Synthes Products Inc
Application granted granted Critical
Publication of ES2552226T3 publication Critical patent/ES2552226T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/51—Umbilical cord; Umbilical cord blood; Umbilical stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/1808—Epidermal growth factor [EGF] urogastrone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/1825—Fibroblast growth factor [FGF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/1833—Hepatocyte growth factor; Scatter factor; Tumor cytotoxic factor II
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/1841—Transforming growth factor [TGF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/185—Nerve growth factor [NGF]; Brain derived neurotrophic factor [BDNF]; Ciliary neurotrophic factor [CNTF]; Glial derived neurotrophic factor [GDNF]; Neurotrophins, e.g. NT-3
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/1858—Platelet-derived growth factor [PDGF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/1858—Platelet-derived growth factor [PDGF]
    • A61K38/1866—Vascular endothelial growth factor [VEGF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/1891—Angiogenesic factors; Angiogenin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/20—Interleukins [IL]
    • A61K38/204—IL-6
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/20—Interleukins [IL]
    • A61K38/2053—IL-8
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/27—Growth hormone [GH], i.e. somatotropin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/04—Drugs for skeletal disorders for non-specific disorders of the connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A61P27/06—Antiglaucoma agents or miotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/21—Chemokines, e.g. MIP-1, MIP-2, RANTES, MCP, PF-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/02—Coculture with; Conditioned medium produced by embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Epidemiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Cell Biology (AREA)
  • Neurosurgery (AREA)
  • Reproductive Health (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Vascular Medicine (AREA)
  • Endocrinology (AREA)
  • Psychology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Rheumatology (AREA)
  • Cardiology (AREA)

Abstract

Población homogénea aislada de células derivadas de tejido de cordón umbilical humano, para su uso en el tratamiento de una afección ósea o cartilaginosa, en la que dicha población celular puede obtenerse mediante digestión enzimática de tejido de cordón umbilical humano exento de sangre con una metaloproteasa de matriz, una proteasa neutra y una enzima mucolítica, y en la que dicha población celular es capaz de autorrenovación y de proliferación en cultivo, tiene el potencial de diferenciarse a células de otros fenotipos, y en la que la población de células: a) produce cada uno de CD10, CD13, CD44, CD73, CD90, PDGFr-alfa y HLA-A,B,C, según se detecta mediante citometría de flujo; y b) no produce ninguno de CD31, CD34, CD45, CD117, CD141 o HLA-DR,DP,DQ, según se detecta mediante citometría de flujo.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
5
15
25
35
45
55
65
reintroducen los factores de crecimiento, y, posteriormente, llevar a cabo el mismo número de duplicaciones que las células equivalentes que se han desarrollado continuamente. Asimismo, cuando las células se congelan en nitrógeno líquido después de diversos números de duplicaciones de la población y, a continuación, se descongelan y cultivan, experimentan sustancialmente el mismo número de duplicaciones que las células mantenidas no congeladas en cultivo. Las células senescentes no son células muertas o moribundas; en realidad son resistentes a la muerte celular programada (apoptosis), y se han mantenido en su estado de no división durante hasta tres años. Estas células están realmente vivas y son metabólicamente activas, pero no se dividen. Aún no se ha descubierto que el estado de no división de las células senescentes sea reversible mediante ningún agente biológico, químico o el viral.
Tal como se utiliza en el presente documento, la expresión Medio de crecimiento se refiere a un medio de cultivo suficiente para que proliferen las células derivadas posparto. El medio de cultivo del Medio de crecimiento contiene preferentemente medio esencial modificado de Dulbecco (DMEM). Más preferentemente, el Medio de crecimiento contiene glucosa. El Medio de crecimiento contiene preferentemente DMEM con bajo contenido de glucosa (DMEM-LG) (Invitrogen, Carlsbad, CA). El Medio de crecimiento contiene preferentemente suero a aproximadamente el 15% (v/v) (por ejemplo, suero bovino fetal, suero bovino definido). El Medio de crecimiento contiene preferentemente al menos un antibiótico y/o un antimicótico (por ejemplo, penicilina, estreptomicina, anfotericina B, gentamicina, nistatina, preferentemente, 50 unidades/mililitro de penicilina G sódica y 50 microgramos/mililitro de sulfato de estreptomicina). El Medio de crecimiento contiene preferentemente 2-mercaptoetanol (Sigma, St. Louis, MO). Lo más preferentemente, el Medio de crecimiento contiene DMEM con bajo contenido de glucosa, suero, 2-mercaptoetanol y un antibiótico.
Tal como se utiliza en el presente documento, condiciones de crecimiento normales se refiere a las condiciones atmosféricas normales que comprenden CO2 a aproximadamente el 5%, una temperatura de aproximadamente 35ºC-39ºC, más preferentemente 37ºC, y una humedad relativa de aproximadamente el 100%.
El término aislado se refiere a una célula, a un componente celular o a una molécula que se ha sacado de su entorno original.
El término aproximadamente se refiere a una aproximación de un valor indicado dentro de un intervalo de ±10%.
Afección (o lesión o enfermedad) ósea es una expresión incluyente que abarca afecciones, trastornos o enfermedades óseas agudas y crónicas, y metabólicas y no metabólicas. La expresión abarca las afecciones debidas a enfermedad o traumatismo o a la incapacidad del tejido para desarrollarse normalmente. Los ejemplos de afecciones óseas incluyen, pero no se limitan a, defectos óseos congénitos, fracturas óseas, lesiones o defectos del menisco, deformación ósea/vertebral, osteosarcoma, mieloma, displasia ósea y escoliosis, osteoporosis, enfermedad periodontal, pérdida ósea dental, osteomalacia, raquitismo, osteítis fibrosa, osteodistrofia renal, fusión vertebral, reconstrucción o extracción de disco vertebral, y la enfermedad ósea de Paget.
Afección (o lesión o enfermedad) cartilaginosa es una expresión incluyente que abarca afecciones, trastornos o enfermedades, agudas y crónicas, del cartílago. La expresión abarca afecciones que incluyen, pero no se limitan a, defectos congénitos, lesiones de menisco, artritis reumatoide, osteoartritis, o una lesión traumática o quirúrgica en el cartílago.
La expresión tratar (o tratamiento de) una afección ósea o cartilaginosa se refiere a mejorar los efectos de,
o retrasar, detener o revertir la evolución de, o retrasar o prevenir la aparición de, una afección ósea o cartilaginosa tal como se define en el presente documento.
La expresión cantidad eficaz se refiere a una concentración de un reactivo o composición farmacéutica, tal como un factor de crecimiento, un agente de diferenciación, un factor trófico, una población celular u otro agente, que es eficaz para producir un resultado deseado, incluido el crecimiento celular y/o la diferenciación in vitro o in vivo, o el tratamiento de una afección ósea o cartilaginosa tal como se describe en el presente documento. Con respecto a los factores de crecimiento, una cantidad eficaz puede variar de aproximadamente 1 nanogramo/mililitro a aproximadamente 1 microgramo/mililitro. Con respecto a las PPDC como se administran a un paciente in vivo, una cantidad eficaz puede variar de tan solo varios cientos o menos, hasta varios millones o más. En formas de realización específicas, una cantidad eficaz puede variar de 103-1011 . Se entenderá que el número de células a administrar variará dependiendo de las características específicas del trastorno a tratar, incluidas, pero no limitadas al tamaño o volumen/superficie total a tratar, así como la proximidad del sitio de administración a la situación de la zona a tratar, entre otros factores familiares para el biólogo médico.
Las expresiones período (o tiempo) eficaz y condiciones eficaces se refieren a un período de tiempo o de otras condiciones controlables (por ejemplo, temperatura, humedad para los métodos in vitro), necesarias o preferentes para que un agente o composición farmacéutica consiga su resultado previsto.
8
imagen7
imagen8
imagen9
imagen10
imagen11
5
15
25
35
45
55
65
IMAGE:4179671, proteína hipotética DKFZp564F013, regulada por disminución en el cáncer de ovario 1 y clon DKFZp547K1113. En las PPDC en las que la expresión, con respecto a una célula humana que es un fibroblasto, una célula madre mesenquimal o una célula de médula ósea de cresta ilíaca, está aumentada para al menos uno de miembro A2 de la superfamilia de lectinas tipo C, tumor de Wilms 1, miembro A2 de la familia de aldehído deshidrogenasa 1, renina, receptor 1 de lipoproteína de baja densidad oxidada, proteína quinasa C zeta, clon IMAGE:4179671, proteína hipotética DKFZp564F013, regulada por disminución en el cáncer de ovario 1 y clon DKFZp547K1113, preferentemente no hay niveles relativos aumentados de al menos uno de interleucina 8; reticulón 1; ligando 1 de quimiocina (motivo C-X-C) (actividad estimuladora del crecimiento de melanoma, alfa); ligando 6 de quimiocina (motivo C-X-C) (proteína 2 quimiotáctica para granulocitos); ligando 3 de quimiocina (motivo C-X-C); y factor de necrosis tumoral, proteína 3 inducida por alfa.
Las PPDC pueden tener expresión que, con respecto a una célula humana que es un fibroblasto, una célula madre mesenquimal o una célula de médula ósea de cresta ilíaca, está reducida para al menos uno de: caja homeótica 2 de la baja estatura; proteína 2 de choque térmico de 27 kDa; ligando 12 de quimiocina (motivo C-X-C) (factor 1 derivado de células del estroma); elastina; ADNc DKFZp586M2022 (del clon DKFZp586M2022); caja homeótica 2 de mesénquima; homólogo 1 de caja homeótica de “sine oculis”; cristalina, alfa B; activador de morfogénesis 2 asociado a “dishevelled”; proteína DKFZP586B2420; similar a neuralin 1; tetranectina; dominio 3 de homología con src y rico en cisteínas; gen 1 de translocación de linfocitos B, antiproliferativa; colesterol 25hidroxilasa; factor de transcripción 3 relacionado con el enanismo; proteína hipotética FLJ23191; receptor de interleucina 11, alfa; potenciador de procolágeno C-endopeptidasa; homólogo 7 de “frizzled”; gen hipotético BC008967; colágeno, tipo VIII, alfa 1; tenascina C; proteína de caja homeótica iroquois 5; hefaestina; integrina, beta 8; glicoproteína 2 de vesícula sináptica; ADNc FLJ12280 fis, clon MAMMA1001744; factor 1 similar al receptor de citocinas; canal de potasio activado por calcio de conductancia intermedia/baja, subfamilia N, miembro 4; integrina, alfa 7; proteína DKFZP586L151; coactivador de la transcripción con motivo de unión a PDZ (TAZ); homólogo 2 de caja homeótica de “sine oculis”; proteína KIAA1034; respuesta de crecimiento precoz 3; caja homeótica de “distal less” 5; proteína hipotética FLJ20373; familia 1 de aldo-ceto reductasas, miembro C3 (3-alfa hidroxiesteroide deshidrogenasa, tipo II); biglicano; fibronectina 1; proencefalina; integrina, tipo beta 1 (con dominios de repetición similares a EGF); clon de ADNc EUROIMAGE 1968422; EphA3; proteína KIAA0367; receptor de péptido natriurético C/guanilato ciclasa C (receptor del péptido atrial natriurético C); proteína hipotética FLJ14054; ADNc DKFZp564B222 (del clon DKFZp564B222); proteína de membrana asociada a vesícula 5; proteína 1 de la matriz extracelular tipo fibulina que contiene EGF; similar a proteína 3 de interacción con BCL2/adenovirus E1B de 19 kDa; proteína 1 de unión a AE; polipéptido 1 de la subunidad VIIa de la citocromo c oxidasa (músculo); neuroblastoma, supresión de tumorigenicidad 1; y proteína 2 de unión al factor de crecimiento insulinoide, 36 kDa; el experto en la materia entenderá que la expresión de una gran diversidad de genes se caracteriza convenientemente en una matriz génica, por ejemplo en un GENECHIP de Affymetrix.
Las PPDC pueden secretar diversos factores bioquímicamente activos, tales como factores de crecimiento, quimiocinas, citocinas y similares. Las células preferentes de la divulgación secretan al menos uno de MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO, MIP1a, RANTES y TIMP1. Las PPDC pueden caracterizarse por su ausencia de secreción de al menos uno de TGF-beta2, ANG2, PDGF-bb, MIP1b, I309, MDC y VEGF, como se detecta mediante ELISA. Se dispone de estas y otras características para identificar y caracterizar las células, y distinguir las células de la invención de otras conocidas en la técnica.
En las formas de realización preferentes, la célula comprende dos o más de las características anteriormente indicadas. Resultan más preferentes las células que comprenden, tres, cuatro o cinco o más de las características. Resultan aún más preferentes las células derivadas posparto que comprenden seis, siete u ocho o más de las características. Actualmente resultan aún más preferentes las células que comprenden las nueve características que se reivindican.
También resultan preferentes actualmente células que producen al menos dos de entre GCP-2, NOGO-A, factor tisular, vimentina y alfa actina de músculo liso. Resultan más preferentes las células que producen tres, cuatro
o cinco de estas proteínas.
El experto en la materia entenderá que los marcadores celulares están sujetos cierta variación en condiciones de crecimiento muy diferentes, y que en general en el presente documento se describen caracterizaciones en Medio de Crecimiento, o variaciones del mismo. Las células derivadas posparto que producen al menos uno, dos, tres o cuatro de entre CD10, CD13, CD44, CD73, CD90, PDGFr-alfa, PD-L2 y HLA-A,B,C son células preferentes de la divulgación. Resultan más preferentes las células que producen cinco, seis, o siete de estos marcadores de superficie celular. Resultan aún más preferentes las células derivadas posparto que pueden producir ocho, nueve o diez de las proteínas marcadoras de superficie celular anteriormente indicadas.
Las PPDC que no producen al menos una, dos, tres o cuatro de las proteínas CD31, CD34, CD45, CD80, CD86, CD117, CD141, CD178, B7-H2, HLA-G y HLA-DR,DP,DQ, según se detecta mediante citometría de flujo, son células preferentes de la divulgación. Resultan preferentes las PPDC que no producen al menos cinco, seis, siete u ocho o más de estos marcadores. Resultan más preferentes las células que no producen al menos nueve o diez de
14
imagen12
imagen13
imagen14
imagen15
5
15
25
35
45
55
65
que necesite tratamiento, por ejemplo, para una afección ósea o cartilaginosa tal como se describe en el presente documento. Como alternativa, en situaciones en las que sea apropiada o deseable la transferencia autóloga, las poblaciones celulares cocultivadas pueden separarse físicamente en cultivo, lo que permite extraer las células autólogas para administrarlas al paciente.
Medio acondicionado de PPDC
Otra forma de realización de la divulgación se ocupa del uso de las PPDC para la producción de un medio acondicionado, ya sea de PPDC indiferenciadas o de PPDC incubadas en condiciones que estimulan la diferenciación a un linaje osteogénico o condrogénico. Tales medios acondicionados se contemplan para su uso en el cultivo de células in vitro o ex vivo, por ejemplo, células madre o progenitoras, incluidas, pero no limitadas a, células de médula ósea, osteoblastos, osteocitos, osteoclastos, células de revestimiento óseo, condroblastos y condrocitos, o in vivo para sustentar a células trasplantadas que comprenden poblaciones homogéneas o heterogéneas de PPDC, osteocitos, osteoblastos, osteoclastos, células de revestimiento óseo, condrocitos, condroblastos y células de médula ósea, por ejemplo.
Aplicaciones terapéuticas de las PPDC
Las PPDC de la invención pueden utilizarse para tratar a pacientes que requieren la reparación o sustitución de tejido óseo o cartilaginoso resultado de una enfermedad o traumatismo o de la incapacidad del tejido para desarrollarse normalmente, o para proporcionar una función cosmética, tal como para aumentar los rasgos faciales u otros rasgos corporales. El tratamiento puede implicar el uso de las células de la invención para producir nuevo tejido cartilaginoso o tejido óseo. Por ejemplo, las células inducidas a diferenciarse condrogénicas o indiferenciadas de la invención pueden utilizarse para tratar una afección cartilaginosa, por ejemplo, la artritis reumatoide o la osteoartritis o una lesión traumática o quirúrgica del cartílago. Como otro ejemplo, las células inducidas a diferenciarse osteogénicas o indiferenciadas de la invención pueden utilizarse para tratar afecciones óseas, incluidas enfermedades óseas metabólicas y no metabólicas. Los ejemplos de afecciones óseas incluyen desgarros meniscales, fusión vertebral, extracción de disco vertebral, reconstrucción vertebral, fracturas óseas, deformación ósea/vertebral, osteosarcoma, mieloma, displasia ósea, escoliosis, osteoporosis, enfermedad periodontal, pérdida de hueso dental, osteomalacia, raquitismo, osteítis fibrosa, osteodistrofia renal y enfermedad ósea de Paget.
Las células de la invención pueden administrarse en solitario o en forma de mezclas con otras células. Las células que pueden administrarse junto con las PPDC incluyen, pero no se limitan a, otras células multipotentes o pluripotentes o condrocitos, condroblastos, osteocitos, osteoblastos, osteoclastos, células de revestimiento óseo o células de médula ósea. Las células de diferentes tipos pueden mezclarse con las PPDC inmediatamente o poco antes de la administración, o pueden cocultivarse juntas durante un período de tiempo antes de la administración.
Las PPDC pueden administrarse con otras moléculas biológicas o fármacos beneficiosos (factores de crecimiento, factores tróficos). Cuando las PPDC se administran con otros agentes, pueden administrarse juntas en una única composición farmacéutica o en composiciones farmacéuticas separadas, simultánea o secuencialmente con los demás agentes (ya sea antes o después de la administración de los demás agentes). Los factores bioactivos que pueden administrarse conjuntamente incluyen agentes antiapoptóticos (por ejemplo, EPO, mimeticuerpo de la EPO, TPO, IGF-I e IGF-II, HGF, inhibidores de caspasas); antiinflamatorios (por ejemplo, inhibidores de p38 MAPK, inhibidores de TGF-beta, estatinas, inhibidores de IL-6 e IL-1, PEMIROLAST, TRANILAST, REMICADE, SIROLIMUS y AINE (fármacos antiinflamatorios no esteroideos, por ejemplo, TEPOXALIN, TOLMENTIN, SUPROFEN); inmunosupresores/inmunomoduladores (por ejemplo, inhibidores de calcineurina, tales como ciclosporina, tacrolimus; inhibidores de mTOR (por ejemplo, SIROLIMUS, EVEROLIMUS); antiproliferativos (por ejemplo, azatioprina, micofenolato de mofetilo); corticosteroides (por ejemplo, prednisolona, hidrocortisona); anticuerpos tales como anticuerpos monoclonales contra IL-2Ralfa (por ejemplo, basiliximab, daclizumab), anticuerpos policlonales contra linfocitos T (por ejemplo, globulina anti-timocitos (ATG); globulina anti-linfocitos (ALG); anticuerpo monoclonal contra linfocitos T, OKT3)); antitrombogénicos (por ejemplo, heparina, derivados de heparina, uroquinasa, PPack (dextrofenilalanina prolina arginina clorometilcetona), compuestos antitrombina, antagonistas de receptores plaquetarios, anticuerpos antitrombina, anticuerpos contra receptores plaquetarios, aspirina, dipiridamol, protamina, hirudina, inhibidores de prostaglandinas e inhibidores plaquetarios); y antioxidantes (por ejemplo, probucol, vitamina A, ácido ascórbico, tocoferol, coenzima Q-10, glutatión, L-cisteína, N-acetilcisteína), así como anestésicos locales. Como otro ejemplo, las células pueden administrarse conjuntamente con un factor inhibidor de cicatrices como se describe en la patente de Estados Unidos Nº 5.827.735.
En una forma de realización, las PPDC se administran como células indiferenciadas, es decir, tal como se cultivan en el Medio de Crecimiento. Como alternativa, en una forma de realización de la divulgación, las PPDC pueden administrarse después de exponerlas en cultivo a condiciones que estimulan la diferenciación hacia un fenotipo deseado, por ejemplo, un fenotipo condrogénico u osteogénico.
Las células de la invención pueden implantarse quirúrgicamente, inyectarse, introducirse (por ejemplo, por medio de un catéter o jeringa), o administrarse de otra manera, directa o indirectamente, al sitio que necesita la
19
imagen16
imagen17
imagen18
imagen19
imagen20
imagen21
imagen22
imagen23
imagen24
imagen25
imagen26
imagen27
imagen28
imagen29
imagen30
imagen31
imagen32
imagen33
imagen34
imagen35
imagen36
imagen37
imagen38
imagen39
imagen40
imagen41
imagen42
imagen43
5
15
25
35
45
55
209763_at
similar a neuralin 1 AL049176
205200_at
tetranectina (proteína de unión a plasminógeno) NM_003278.1
205743_at
dominio tres de homología con src (SH3) y rico en cisteínas NM_003149.1
200921_s_at
gen 1 de translocación de linfocitos B, antiproliferativa NM_001731.1
206932_at
colesterol 25-hidroxilasa NM_003956.1
204198_s_at
factor de transcripción 3 relacionado con el enanismo AA541630
219747_at
proteína hipotética FLJ23191 NM_024574.1
204773_at
receptor de interleucina 11, alfa NM_004512.1
202465_at
potenciador de procolágeno C-endopeptidasa NM_002593.2
203706_s_at
homólogo 7 de “frizzled” (Drosophila) NM_003507.1
212736_at
gen hipotético BC008967 BE299456
214587_at
colágeno, tipo VIII, alfa 1 BE877796
201645_at
tenascina C (hexabraquion) NM_002160.1
210239_at
proteína de caja homeótica iroquois 5 U90304.1
203903_s_at
hefaestina NM_014799.1
205816_at
integrina, beta 8 NM_002214.1
203069_at
glicoproteína 2 de vesícula sináptica NM_014849.1
213909_at
ADNc de Homo sapiens FLJ12280 fis, clon MAMMA1001744 AU147799
206315_at
factor 1 similar al receptor de citocinas NM_004750.1
204401_at
canal de potasio activado por calcio de conductancia intermedia/baja, subfamilia N, miembro 4 NM_002250.1
216331_at
integrina, alfa 7 AK022548.1
209663_s_at
integrina, alfa 7 AF072132.1
213125_at
proteína DKFZP586L151 AW007573
202133_at
coactivador de la transcripción con motivo de unión a PDZ (TAZ) AA081084
206511_s_at
homólogo 2 de caja homeótica de “sine oculis” (Drosophila) NM_016932.1
213435_at
proteína KIAA1034 AB028957.1
206115_at
respuesta de crecimiento precoz 3 NM_004430.1
213707_s_at
caja homeótica de “distal less” 5 NM_005221.3
218181_s_at
proteína hipotética FLJ20373 NM_017792.1
209160_at
familia 1 de aldo-ceto reductasas, miembro C3 (3-alfa hidroxiesteroide deshidrogenasa, tipo II) AB018580.1
213905_x_at
biglicano AA845258
201261_x_at
biglicano BC002416.1
202132_at
coactivador de la transcripción con motivo de unión a PDZ (TAZ) AA081084
214701_s_at
fibronectina 1 AJ276395.1
213791_at
proencefalina NM_006211.1
205422_s_at
integrina, tipo beta 1 (con dominios de repetición similares a EGF) NM_004791.1
214927_at
clon de ADNc de inserto de longitud completa de ARNm de Homo sapiens EUROIMAGE 1968422 AL359052.1
206070_s_at
EphA3 AF213459.1
212805_at
proteína KIAA0367 AB002365.1
219789_at
receptor de péptido natriurético C/guanilato ciclasa C (receptor del péptido atrial natriurético C) A1628360
219054_at
proteína hipotética FLJ14054 NM_024563.1
213429_at
ARNm de Homo sapiens; ADNc DKFZp564B222 (del clon DKFZp564B222) AW025579
204929_s_at
proteína de membrana asociada a vesícula 5 (miobrevina) NM_006634.1
201843_s_at
proteína 1 de la matriz extracelular tipo fibulina que contiene EGF NM_004105.2
221478_at
similar a proteína 3 de interacción con BCL2/adenovirus E1B de 19 kDa AL132665.1
201792_at
proteína 1 de unión a AE NM_001129.2
204570_at
polipéptido 1 de la subunidad VIIa de la citocromo c oxidasa (músculo) NM_001864.1
48
201621_at
neuroblastoma, supresión de tumorigenicidad 1 NM_005380.1
202718_at
proteína 2 de unión al factor de crecimiento insulinoide, 36 kDa NM_000597
Las Tablas 8-6, 8-7 y 8-8 muestran el aumento de la expresión de los genes en los fibroblastos humanos (Tabla 8-6), las células ICBM (Tabla 8-7) y las MSC (Tabla 8-8).
15
25
35

Tabla 8-6. Genes que demostraron tener una expresión aumentada en los fibroblastos en comparación con las demás líneas celulares ensayadas.
Genes aumentados en fibroblastos
Fosfatasa 2 de doble especificidad
Proteína KIAA0527
ADNc de Homo sapiens: FLJ23224 fis, clon ADSU02206
Dineína, citoplasmática, polipéptido intermedio 1
Anquirina 3, nódulo de Ranvier (anquirina G)
Inhibina, beta A (activina A, activina AB, polipéptido alfa)
Ectonucleótido pirofosfatasa/fosfodiesterasa 4 (función supuesta)
Proteína KIAA1053
Proteína 1A asociada a microtúbulos
Proteína con dedos de zinc 41
Proteína HSPC019
ADNc de Homo sapiens: FLJ23564 fis, clon LNG10773
ARNm de Homo sapiens; ADNc DKFZp564A072 (del clon DKFZp564A072)
Proteína LIM (similar a enigma de unión a proteína quinasa C de rata)
Inhibidor del potenciador del gen del polipéptido de cadena ligera kappa en linfocitos B, proteína asociada al complejo quinasa
Proteína hipotética FLJ22004
Secuencia de ARNm humano (clon CTG-A4)
ESTs, Moderadamente similar al factor 2 similar al receptor de citocinas; precursor CRL2 del receptor de citocinas [Homo sapiens]
Factor de crecimiento transformante, beta 2
Proteína hipotética MGC29643
Antígeno identificado por el anticuerpo monoclonal MRC OX-2
Tabla 8-7. Genes que demostraron tener una expresión aumentada en las células derivadas de ICBM en comparación con las demás líneas celulares ensayadas.
45
55
Genes aumentados en células ICBM
Proteína de repetición de anquirina cardiaca
ORF de la región MHC de clase I
Integrina, alfa 10
Proteína hipotética FLJ22362
UDP-N-acetil-alfa-D-galactosamina:polipéptido N-acetilgalactosaminiltransferasa 3 (GalNAc-T3)
Proteína 44 inducida por interferón
SRY (región determinante del sexo del cromosoma Y)-caja 9 (displasia campomélica, reversión sexual autosómica)
Proteína asociada a queratina 1-1
Tipo hipocalcina 1
Jagged 1 (síndrome de Alagille)
Proteoglicano 1, gránulo de secreción

Tabla 8-8. Genes que demostraron tener una expresión aumentada en las células MSC en comparación con las demás líneas celulares ensayadas.
Genes aumentados en las células MSC
interleucina 26 maltasa-glucoamilasa (alfa-glucosidasa)
49
imagen44
imagen45
imagen46
imagen47
imagen48
imagen49
imagen50
alogénico de control positivo. Los seis donantes de sangre restantes se seleccionaron como receptores. El donante alogénico de control positivo y líneas celulares derivadas de la placenta se trataron con mitomicina C y se cultivaron en una reacción linfocitaria mixta con los seis receptores alogénicos individuales. Las reacciones se realizaron por triplicado utilizando dos placas de cultivo celular con tres receptores por placa (Tabla 11-2). El índice medio de estimulación varió entre 1,3 (placa 2) y 3 (placa 1) y los donantes alogénicos de control positivo variaron entre 46,25 (placa 2) y 279 (placa 1) (Tabla 11-3).
15
25
35
45
55

Tabla 11-2. Datos de la reacción linfocitaria mixta -Línea celular B (placenta)
DMP para el ensayo de proliferación
ID de placa: Placa 1
Número del análisis
Sistema de cultivo Replicaciones
1
2 3 Media DE CV
IM03-7769
Medida inicial de la proliferación del receptor 79 119 138 112,0 30,12 26,9
Control de autoestimulación (células autólogas tratadas con mitomicina C)
241 272 175 229,3 49,54 21,6
Donante alogénico de MLR IM03-7768 (tratadas con mitomicina C)
23971 22352 20921 22414,7 1525,97 6,8
MLR con línea celular (célula tipo B tratada con mitomicina C)
664 559 1090 771,0 281,21 36,5
SI (donante)
200
SI (línea celular)
7
IM03-7770
Medida inicial de la proliferación del receptor 206 134 262 200,7 64,17 32,0
Control de autoestimulación (células autólogas tratadas con mitomicina C)
1091 602 524 739,0 307,33 41,6
Donante alogénico de MLR IM03-7768 (tratadas con mitomicina C)
45005 43729 44071 44268,3 660,49 1,5
MLR con línea celular (célula tipo B tratada con mitomicina C)
533 2582 2376 1830,3 1128,24 61,6
SI (donante)
221
57
15
25
35
45
55
SI (línea celular)
9
IM03-7771
Medida inicial de la proliferación del receptor 157 87 128 124,0 35,17 28,4
Control de autoestimulación (células autólogas tratadas con mitomicina C)
293 138 508 313,0 185,81 59,4
Donante alogénico de MLR IM03-7768 (tratadas con mitomicina C)
24497 34348 31388 30077,7 5054,53 16,8
MLR con línea celular (célula tipo B tratada con mitomicina C)
601 643 a 622,0 29,70 4,8
SI (donante)
243
SI (línea celular)
5
IM03-7772
Medida inicial de la proliferación del receptor 56 98 51 68,3 25,81 37,8
Control de autoestimulación (células autólogas tratadas con mitomicina C)
133 120 213 155,3 50,36 32,4
Donante alogénico de MLR IM03-7768 (tratadas con mitomicina C)
14222 20076 22168 18822,0 4118,75 21,9
MLR con línea celular (célula tipo B tratada con mitomicina C)
a a a a a a
SI (donante)
275
SI (línea celular)
a
IM03-7768 (donante alogénico)
Medida inicial de la proliferación del receptor 84 242 208 178,0 83,16 46,7
Control de autoestimulación (células autólogas tratadas con mitomicina)
361 617 304 427,3 166,71 39,0
Línea celular tipo B
Medida inicial de la proliferación del receptor 126 124 143 131,0 10,44 8,0
Control de autoestimulación (células autólogas tratadas con mitomicina)
822 1075 487 794,7 294,95 37,1
ID de placa: Placa 2
Número del análisis
Sistema de cultivo Replicaciones
1
2 3 Media DE CV
IM03-7773
Medida inicial de la proliferación del receptor 908 181 330 473,0 384,02 81,2
Control de autoestimulación (células autólogas tratadas con mitomicina C)
269 405 572 415,3 151,76 36,5
Donante alogénico de MLR IM03-7768 (tratadas con mitomicina C)
29151 28691 28315 28719,0 418,70 1,5
MLR con línea celular (célula tipo B tratada con mitomicina C)
567 732 905 734,7 169,02 23,0
SI (donante)
61
SI (línea celular)
2
58
5
15
25
35
45
IM03-7774
Medida inicial de la proliferación del receptor 893 1376 185 818,0 599,03 73, 2
Control de autoestimulación (células autólogas tratadas con mitomicina C)
261 381 568 403,3 154,71 38, 4
Donante alogénico de MLR IM03-7768 (tratadas con mitomicina C)
5310 1 4283 9 4828 3 48074, 3 5134,1 8 10, 7
MLR con línea celular (célula tipo B tratada con mitomicina C)
515 789 294 532,7 247,97 46, 6
SI (donante)
59
SI (línea celular)
1
IM03-7775
Medida inicial de la proliferación del receptor 1272 300 544 705,3 505,69 71, 7
Control de autoestimulación (células autólogas tratadas con mitomicina C)
232 199 484 305,0 155,89 51, 1
Donante alogénico de MLR IM03-7768 (tratadas con mitomicina C)
2355 4 1052 3 2896 5 21014, 0 9479,7 4 45, 1
MLR con línea celular (célula tipo B tratada con mitomicina C)
768 924 563 751,7 181,05 24, 1
SI (donante)
30
SI (línea celular)
1
IM03-7776
Medida inicial de la proliferación del receptor 1530 137 1046 904,3 707,22 78, 2
Control de autoestimulación (células autólogas tratadas con mitomicina C)
420 218 394 344,0 109,89 31, 9
Donante alogénico de MLR IM03-7768 (tratadas con mitomicina C)
2889 3 3249 3 3474 6 32044, 0 2952,2 2 9,2
MLR con línea celular (célula tipo B tratada con mitomicina C)
a a a a a a
SI (donante)
35
SI (línea celular)
a

Tabla 11-3. Índice medio de estimulación de células de la placenta y un donante alogénico en una reacción linfocitaria mixta con seis receptores alogénicos individuales.
Índice medio de estimulación
Receptor
Placenta
Placa 1 (receptores 1-3)
279 3
Placa 2 (receptores 4-6)
46,25 1,3
Reacción linfocitaria mixta -cordón umbilical. Se sometieron a criba seis donantes de sangre voluntarios humanos para identificar un solo donante alogénico que presentara una fuerte respuesta de proliferación en una reacción linfocitaria mixta con los otros cinco donantes de sangre. Este donante se seleccionó como el donante alogénico de control positivo. Los cinco donantes de sangre restantes se seleccionaron como receptores. El donante alogénico de control positivo y las líneas celulares derivadas de cordón umbilical se trataron con mitomicina C y se cultivaron en una reacción linfocitaria mixta con los cinco receptores alogénicos individuales. Las reacciones
65 se realizaron por triplicado utilizando dos placas de cultivo celular con tres receptores por placa (Tabla 11-4). El
59
índice medio de estimulación varió entre 6,5 (placa 1) y 9 (placa 2) y los donantes alogénicos de control positivo variaron entre 42,75 (placa 1) y 70 (placa 2) (Tabla 11-5).
5
15
25
35
45
55

Tabla 11-4. Datos de la reacción linfocitaria mixta -Línea Celular A (cordón umbilical)
DMP para el ensayo de proliferación ID de placa: Placa 1
Número del análisis
Sistema de cultivo Replicaciones 1 2 3 Media DE CV
Medida inicial de la
proliferación del receptor Control de autoestimulación
1074 406 391 623,7 390,07 62,5
(células autólogas tratadas
672 510 1402 861,3 475,19 55,2
IM04-2478
con mitomicina C) Donante alogénico de
MLR IM04-2477 (tratadas con mitomicina C) MLR con línea celular
43777 48391 38231 43466,3 5087,12 11,7
(célula tipo A tratada con mitomicina C)
2914 5622 6109 4881,7 1721,36 35,3
SI (donante) SI (línea celular)
70 8
Medida inicial de la
proliferación del receptor Control de autoestimulación
530 508 527 521,7 11,93 2,3
(células autólogas tratadas
701 567 1111 793,0 283,43 35,7
IM04-2479
con mitomicina C) Donante alogénico de
MLR IM04-2477 (tratadas con mitomicina C) MLR con línea celular
25593 24732 22707 24344,0 1481,61 6,1
(célula tipo A tratada con mitomicina C)
5086 3932 1497 3505,0 1832,21 52,3
SI (donante) SI (línea celular)
47 7
Medida inicial de la
proliferación del receptor Control de autoestimulación
1192 854 1330 1125,3 244,90 21,8
(células autólogas tratadas
2963 993 2197 2051,0 993,08 48,4
IM04-2480
con mitomicina C) Donante alogénico de
MLR IM04-2477 (tratadas con mitomicina C) MLR con línea celular
25416 29721 23757 26298,0 3078,27 11,7
(célula tipo A tratada con mitomicina C)
2596 5076 3426 3699,3 1262,39 34,1
60
5
15
25
35
45
55
SI (donante) SI (línea celular)
23 3
Medida inicial de la
proliferación del receptor Control de autoestimulación
695 451 555 567,0 122,44 21,6
(células autólogas tratadas
738 1252 464 818,0 400,04 48,9
IM04-2481
con mitomicina C) Donante alogénico de
MLR IM04-2477 (tratadas con mitomicina C) MLR con línea celular
13177 24885 15444 17835,3 6209,52 34,8
(célula tipo A tratada con mitomicina C)
4495 3671 4674 4280,0 534,95 12,5
SI (donante) SI (línea celular)
31 8
ID de placa: Placa 2
Número del análisis
Sistema de cultivo Replicaciones 1 2 3 Media DE CV
Medida inicial de la
proliferación del receptor Control de autoestimulación
432 533 274 413,0 130,54 31,6
(células autólogas tratadas
1459 633 598 896,7 487,31 54,3
IM04-2482
con mitomicina C) Donante alogénico de
MLR IM04-2477 (tratadas con mitomicina C) MLR con línea celular
24286 30823 31346 28818,3 3933,82 13,7
(célula tipo A tratada con mitomicina C)
2762 1502 6723 3662,3 2724,46 74,4
SI (donante) SI (línea celular)
70 9
IM04-2477 (donante alogénico)
Medida inicial de la proliferación del receptor Control de autoestimulación (células autólogas tratadas con mitomicina) 312 419 349 567 604 374 360,0 515,0 54,34 123,50 15,1 24,0
Medida inicial de la
Línea celular tipo A
proliferación del receptor Control de autoestimulación (células autólogas tratadas con mitomicina) 5101 3735 2973 1924 4570 2153 3936,3 2882,3 1078,19 1466,04 27,4 50,9

Tabla 11-5. Índice medio de estimulación de células derivadas de cordón umbilical y un donante alogénico en una reacción linfocitaria mixta con cinco receptores alogénicos individuales.
Índice medio de estimulación
Receptor
Cordón umbilical
Placa 1 (receptores 1-4)
42,75 6,5
Placa 2 (receptor 5)
70 9
61
imagen51
imagen52
imagen53
imagen54
imagen55
imagen56
imagen57
imagen58
imagen59
imagen60
imagen61
imagen62
imagen63
imagen64
imagen65
imagen66
imagen67

Claims (1)

  1. imagen1
ES10183911.6T 2003-06-27 2004-06-25 Reparación y regeneración de cartílago y hueso utilizando células derivadas posparto Expired - Lifetime ES2552226T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48326403P 2003-06-27 2003-06-27
US483264P 2003-06-27

Publications (1)

Publication Number Publication Date
ES2552226T3 true ES2552226T3 (es) 2015-11-26

Family

ID=38456561

Family Applications (14)

Application Number Title Priority Date Filing Date
ES10183911.6T Expired - Lifetime ES2552226T3 (es) 2003-06-27 2004-06-25 Reparación y regeneración de cartílago y hueso utilizando células derivadas posparto
ES10184593.1T Expired - Lifetime ES2542070T3 (es) 2003-06-27 2004-06-25 Células posparto derivadas de tejido del cordón umbilical y métodos de preparación y uso de las mismas para la reparación y regeneración de tejido blando
ES04777286.8T Expired - Lifetime ES2582342T3 (es) 2003-06-27 2004-06-25 Reparación y regeneración de tejido blando usando células derivadas del posparto
ES10183053.7T Expired - Lifetime ES2550267T3 (es) 2003-06-27 2004-06-25 Células derivadas de cordón umbilical post-parto para su uso en el tratamiento de enfermedad del corazón y el sistema circulatorio
ES04777231.4T Expired - Lifetime ES2597837T3 (es) 2003-06-27 2004-06-25 Células posparto derivadas de tejido de la placenta, y métodos de fabricación y utilización de los mismos
ES10184137.7T Expired - Lifetime ES2542069T3 (es) 2003-06-27 2004-06-25 Regeneración y reparación de tejido neural usando células del postparto derivadas del cordón umbilical
ES04777287.6T Expired - Lifetime ES2564045T3 (es) 2003-06-27 2004-06-25 Células derivadas del post-parto para su uso en el tratamiento de enfermedad del corazón y el sistema circulatorio
ES04777235.5T Expired - Lifetime ES2568463T3 (es) 2003-06-27 2004-06-25 Regeneración y reparación de tejido neural usando células del postparto derivadas del cordon umbilical
ES04777234.8T Expired - Lifetime ES2600555T3 (es) 2003-06-27 2004-06-25 Reparación y regeneración de tejido ocular usando células derivadas del post parto
ES04756395.2T Expired - Lifetime ES2564044T3 (es) 2003-06-27 2004-06-25 Células posparto derivadas de tejido del cordón umbilical y métodos de preparación y uso de las mismas
ES10184306.8T Expired - Lifetime ES2554343T3 (es) 2003-06-27 2004-06-25 Células posparto derivadas de tejido del cordón umbilical y métodos de preparación y uso de las mismas
ES04809466.8T Expired - Lifetime ES2565582T3 (es) 2003-06-27 2004-06-25 Reparación y regeneración de cartílago y hueso utilizando células derivadas posparto
ES10184221.9T Expired - Lifetime ES2541604T3 (es) 2003-06-27 2004-06-25 Reparación y regeneración de tejido ocular usando células derivadas del cordón umbilical post parto
ES10184796.0T Expired - Lifetime ES2569780T3 (es) 2003-06-27 2004-06-25 Células posparto derivadas de tejido placentario y métodos de preparación y uso de las mismas

Family Applications After (13)

Application Number Title Priority Date Filing Date
ES10184593.1T Expired - Lifetime ES2542070T3 (es) 2003-06-27 2004-06-25 Células posparto derivadas de tejido del cordón umbilical y métodos de preparación y uso de las mismas para la reparación y regeneración de tejido blando
ES04777286.8T Expired - Lifetime ES2582342T3 (es) 2003-06-27 2004-06-25 Reparación y regeneración de tejido blando usando células derivadas del posparto
ES10183053.7T Expired - Lifetime ES2550267T3 (es) 2003-06-27 2004-06-25 Células derivadas de cordón umbilical post-parto para su uso en el tratamiento de enfermedad del corazón y el sistema circulatorio
ES04777231.4T Expired - Lifetime ES2597837T3 (es) 2003-06-27 2004-06-25 Células posparto derivadas de tejido de la placenta, y métodos de fabricación y utilización de los mismos
ES10184137.7T Expired - Lifetime ES2542069T3 (es) 2003-06-27 2004-06-25 Regeneración y reparación de tejido neural usando células del postparto derivadas del cordón umbilical
ES04777287.6T Expired - Lifetime ES2564045T3 (es) 2003-06-27 2004-06-25 Células derivadas del post-parto para su uso en el tratamiento de enfermedad del corazón y el sistema circulatorio
ES04777235.5T Expired - Lifetime ES2568463T3 (es) 2003-06-27 2004-06-25 Regeneración y reparación de tejido neural usando células del postparto derivadas del cordon umbilical
ES04777234.8T Expired - Lifetime ES2600555T3 (es) 2003-06-27 2004-06-25 Reparación y regeneración de tejido ocular usando células derivadas del post parto
ES04756395.2T Expired - Lifetime ES2564044T3 (es) 2003-06-27 2004-06-25 Células posparto derivadas de tejido del cordón umbilical y métodos de preparación y uso de las mismas
ES10184306.8T Expired - Lifetime ES2554343T3 (es) 2003-06-27 2004-06-25 Células posparto derivadas de tejido del cordón umbilical y métodos de preparación y uso de las mismas
ES04809466.8T Expired - Lifetime ES2565582T3 (es) 2003-06-27 2004-06-25 Reparación y regeneración de cartílago y hueso utilizando células derivadas posparto
ES10184221.9T Expired - Lifetime ES2541604T3 (es) 2003-06-27 2004-06-25 Reparación y regeneración de tejido ocular usando células derivadas del cordón umbilical post parto
ES10184796.0T Expired - Lifetime ES2569780T3 (es) 2003-06-27 2004-06-25 Células posparto derivadas de tejido placentario y métodos de preparación y uso de las mismas

Country Status (8)

Country Link
US (28) US9579351B2 (es)
EP (14) EP2338982B1 (es)
JP (7) JP5148873B2 (es)
AU (7) AU2004252567B2 (es)
CA (7) CA2530732C (es)
ES (14) ES2552226T3 (es)
PL (14) PL2338980T3 (es)
WO (7) WO2005001077A2 (es)

Families Citing this family (577)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080077251A1 (en) * 1999-06-07 2008-03-27 Chen Silvia S Cleaning and devitalization of cartilage
US8563232B2 (en) 2000-09-12 2013-10-22 Lifenet Health Process for devitalizing soft-tissue engineered medical implants, and devitalized soft-tissue medical implants produced
US20020095157A1 (en) * 1999-07-23 2002-07-18 Bowman Steven M. Graft fixation device combination
US6179840B1 (en) 1999-07-23 2001-01-30 Ethicon, Inc. Graft fixation device and method
US8147824B2 (en) 1999-08-05 2012-04-03 Athersys, Inc. Immunomodulatory properties of multipotent adult progenitor cells and uses thereof
US7311905B2 (en) * 2002-02-13 2007-12-25 Anthrogenesis Corporation Embryonic-like stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells
US20080152629A1 (en) * 2000-12-06 2008-06-26 James Edinger Placental stem cell populations
DK1349918T3 (da) 2000-12-06 2014-11-10 Anthrogenesis Corp Fremgangsmåde til indsamling af stamceller fra moderkagen
US7311904B2 (en) * 2001-02-14 2007-12-25 Anthrogenesis Corporation Tissue matrices comprising placental stem cells, and methods of making the same
CA2365376C (en) 2000-12-21 2006-03-28 Ethicon, Inc. Use of reinforced foam implants with enhanced integrity for soft tissue repair and regeneration
IL157350A0 (en) * 2001-02-14 2004-02-19 Anthrogenesis Corp Post-partum mammalian placenta, its use and placental stem cells therefrom
WO2003008535A2 (en) * 2001-07-20 2003-01-30 Technion Research And Development Foundation Ltd. Methods of generating human cardiac cells and tissues and uses thereof
US7677565B2 (en) 2001-09-28 2010-03-16 Shuffle Master, Inc Card shuffler with card rank and value reading capability
US7736892B2 (en) 2002-02-25 2010-06-15 Kansas State University Research Foundation Cultures, products and methods using umbilical cord matrix cells
US20080299090A1 (en) * 2002-02-25 2008-12-04 Kansas State University Research Foundation Use Of Umbilical Cord Matrix Cells
US20080003258A1 (en) * 2002-10-16 2008-01-03 Marcum Frank D Composition and Method for Treating Rheumatoid Arthritis
US7824701B2 (en) * 2002-10-18 2010-11-02 Ethicon, Inc. Biocompatible scaffold for ligament or tendon repair
US20040078090A1 (en) * 2002-10-18 2004-04-22 Francois Binette Biocompatible scaffolds with tissue fragments
JP2006509770A (ja) * 2002-11-26 2006-03-23 アンソロジェネシス コーポレーション 細胞治療学、細胞治療単位、およびそれらを利用した治療法
WO2004069172A2 (en) 2003-01-30 2004-08-19 The Government of the United States of America as represented by the Department of Veterans Affairs Multilineage-inducible cells and uses thereof
JP4790592B2 (ja) 2003-02-11 2011-10-12 ダビース，ジヨン・イー ヒト臍帯のウォートンジェリーからの前駆細胞
JP2006517975A (ja) * 2003-02-13 2006-08-03 アンスロジェネシス コーポレーション 疾患、障害または症状を患っている個体を治療するための臍帯血の使用
US8197837B2 (en) 2003-03-07 2012-06-12 Depuy Mitek, Inc. Method of preparation of bioabsorbable porous reinforced tissue implants and implants thereof
EP1620827B1 (en) * 2003-04-24 2010-06-02 Koninklijke Philips Electronics N.V. Non-invasive left ventricular volume determination
US9572840B2 (en) 2003-06-27 2017-02-21 DePuy Synthes Products, Inc. Regeneration and repair of neural tissue using postpartum-derived cells
ES2552226T3 (es) 2003-06-27 2015-11-26 DePuy Synthes Products, Inc. Reparación y regeneración de cartílago y hueso utilizando células derivadas posparto
US9592258B2 (en) 2003-06-27 2017-03-14 DePuy Synthes Products, Inc. Treatment of neurological injury by administration of human umbilical cord tissue-derived cells
US8790637B2 (en) 2003-06-27 2014-07-29 DePuy Synthes Products, LLC Repair and regeneration of ocular tissue using postpartum-derived cells
US8491883B2 (en) * 2003-06-27 2013-07-23 Advanced Technologies And Regenerative Medicine, Llc Treatment of amyotrophic lateral sclerosis using umbilical derived cells
US7875272B2 (en) 2003-06-27 2011-01-25 Ethicon, Incorporated Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells
US20060223177A1 (en) 2003-06-27 2006-10-05 Ethicon Inc. Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
US8518390B2 (en) 2003-06-27 2013-08-27 Advanced Technologies And Regenerative Medicine, Llc Treatment of stroke and other acute neural degenerative disorders via intranasal administration of umbilical cord-derived cells
US8226715B2 (en) 2003-06-30 2012-07-24 Depuy Mitek, Inc. Scaffold for connective tissue repair
US10583220B2 (en) 2003-08-11 2020-03-10 DePuy Synthes Products, Inc. Method and apparatus for resurfacing an articular surface
DK1653994T3 (da) * 2003-08-12 2010-03-15 Tigenix Nv Anvendelse af CXCL6 chemokin i forebyggelsen eller gendannelse af bruskdefekter
US8043614B2 (en) 2004-03-09 2011-10-25 Ahlfors Jan-Eric W Autogenic living scaffolds and living tissue matrices: methods and uses thereof
GB0321337D0 (en) * 2003-09-11 2003-10-15 Massone Mobile Advertising Sys Method and system for distributing advertisements
US7316822B2 (en) * 2003-11-26 2008-01-08 Ethicon, Inc. Conformable tissue repair implant capable of injection delivery
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
EP1694328A4 (en) * 2003-12-02 2010-02-17 Celgene Corp METHOD AND COMPOSITIONS FOR THE TREATMENT AND SUPPLY OF HEMOGLOBINOPATHY AND ANEMIA
US7901461B2 (en) * 2003-12-05 2011-03-08 Ethicon, Inc. Viable tissue repair implants and methods of use
JP2005168360A (ja) * 2003-12-09 2005-06-30 Olympus Corp 生体組織補填体の検査方法、装置、細胞培養容器および培養状態検査方法
US20050176139A1 (en) * 2004-01-12 2005-08-11 Yao-Chang Chen Placental stem cell and methods thereof
WO2005071066A1 (en) * 2004-01-23 2005-08-04 Board Of Regents, The University Of Texas System Methods and compositions for preparing pancreatic insulin secreting cells
US11395865B2 (en) 2004-02-09 2022-07-26 DePuy Synthes Products, Inc. Scaffolds with viable tissue
WO2005093044A1 (en) 2004-03-22 2005-10-06 Osiris Therapeutics, Inc. Mesenchymal stem cells and uses therefor
US8137686B2 (en) 2004-04-20 2012-03-20 Depuy Mitek, Inc. Nonwoven tissue scaffold
US8221780B2 (en) * 2004-04-20 2012-07-17 Depuy Mitek, Inc. Nonwoven tissue scaffold
US7622108B2 (en) * 2004-04-23 2009-11-24 Bioe, Inc. Multi-lineage progenitor cells
CA2563518C (en) * 2004-04-23 2014-09-02 Bioe, Inc. Multi-lineage progenitor cells
US8765119B2 (en) * 2004-05-06 2014-07-01 University Of South Florida Treating amyotrophic lateral sclerosis (ALS)with isolated aldehyde dehydrogenase-positive umbilical cord blood cells
US20060083732A1 (en) * 2004-06-30 2006-04-20 Arlene Gwon Hyaluronic acid in the enhancement of lens regeneration
US7794697B2 (en) 2004-06-30 2010-09-14 Abbott Medical Optics Inc. Enhancement of lens regeneration using materials comprising polysiloxane polymers
US8802651B2 (en) * 2004-06-30 2014-08-12 Abbott Medical Optics Inc. Hyaluronic acid in the enhancement of lens regeneration
AU2005273077B2 (en) 2004-08-16 2011-02-24 Cellresearch Corporation Pte Ltd Isolation of stem/progenitor cells from amniotic membrane of umbilical cord.
US20060045872A1 (en) 2004-08-25 2006-03-02 Universidad Autonoma De Madrid Ciudad Universitaria de Cantoblanco Use of adipose tissue-derived stromal stem cells in treating fistula
US20060069008A1 (en) * 2004-09-28 2006-03-30 Sanjay Mistry Treatment of neurological deficits in the striatum or substanta nigra pars compacta
US8039258B2 (en) * 2004-09-28 2011-10-18 Ethicon, Inc. Tissue-engineering scaffolds containing self-assembled-peptide hydrogels
US20080038316A1 (en) * 2004-10-01 2008-02-14 Wong Vernon G Conveniently implantable sustained release drug compositions
US7473678B2 (en) 2004-10-14 2009-01-06 Biomimetic Therapeutics, Inc. Platelet-derived growth factor compositions and methods of use thereof
US7842304B2 (en) * 2004-10-29 2010-11-30 Nexeon Medsystems, Inc. Methods and apparatus for treating an injured nerve pathway
US11660317B2 (en) 2004-11-08 2023-05-30 The Johns Hopkins University Compositions comprising cardiosphere-derived cells for use in cell therapy
US8017395B2 (en) * 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
US20060166361A1 (en) * 2004-12-21 2006-07-27 Agnieszka Seyda Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same
WO2006101548A2 (en) * 2004-12-21 2006-09-28 Ethicon, Inc. Postpartum cells derived from umbilical cord tissue, and methods of making, culturing, and using the same
US20060153815A1 (en) * 2004-12-21 2006-07-13 Agnieszka Seyda Tissue engineering devices for the repair and regeneration of tissue
EP1831355A2 (en) * 2004-12-23 2007-09-12 Ethicon, Inc. Soft tissue repair and regeneration using postpartum-derived cells and cell products
WO2006071773A2 (en) * 2004-12-23 2006-07-06 Ethicon Incoporated Treatment of osteochondral diseases using postpartum-derived cells and products thereof
CA2592435C (en) 2004-12-23 2017-03-28 DePuy Synthes Products, Inc. Treatment of stroke and other acute neural degenerative disorders using postpartum derived cells
US20060182724A1 (en) * 2005-02-15 2006-08-17 Riordan Neil H Method for expansion of stem cells
JP4783909B2 (ja) * 2005-03-04 2011-09-28 国立大学法人京都大学 心臓組織由来の多能性幹細胞
CN103361302A (zh) 2005-03-31 2013-10-23 斯丹姆涅恩有限公司 从高度纯化来自羊膜的细胞群获得的细胞裂解产物的组合物
US20060222634A1 (en) 2005-03-31 2006-10-05 Clarke Diana L Amnion-derived cell compositions, methods of making and uses thereof
US8153430B2 (en) * 2005-03-31 2012-04-10 Stemnion, Inc. Methods related to surgery
US8999706B2 (en) * 2005-04-12 2015-04-07 The Trustees Of The University Of Pennsylvania Methods for preparation of human hair-follicle derived multipotent adult stem cells
WO2006113731A2 (en) * 2005-04-20 2006-10-26 University Of Florida Research Foundation, Inc. Bone marrow-derived neurogenic cells and uses thereof
US20100087546A1 (en) * 2005-04-20 2010-04-08 Biogenic Innovations, Llc Use of dimethyl sulfone (msm) to reduce homocysteine levels
KR20080007380A (ko) * 2005-04-25 2008-01-18 메사추세츠 인스티튜트 오브 테크놀로지 지혈 및 다른 생리학적 활성을 촉진하기 위한 조성물 및방법
AU2006202209B2 (en) * 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
CN101484575B (zh) * 2005-06-08 2013-10-02 森托科尔公司 用于眼变性的细胞疗法
GB0511723D0 (en) * 2005-06-09 2005-07-13 Smith & Nephew Placental stem cells
WO2006135899A2 (en) * 2005-06-13 2006-12-21 The Johns Hopkins University Survival, differentiation and structural intergration of human neural stem cells grafted into the adult spinal cord
RU2284190C1 (ru) * 2005-06-14 2006-09-27 Дмитрий Дмитриевич Генкин Способ лечения ишемической болезни сердца, или инфаркта миокарда и его последствий, или ишемии мозга, вызванной атеросклерозом или острым нарушением мозгового кровообращения, и ее последствий, или ишемии нижних конечностей, вызванной атеросклерозом
US7838503B2 (en) * 2005-06-15 2010-11-23 Children's Medical Center Corporation Methods for extending the replicative lifespan of cells
US20140336514A1 (en) * 2005-08-05 2014-11-13 Gholam A. Peyman Methods to regulate polarization and enhance function of cells
US20150119794A1 (en) * 2005-08-05 2015-04-30 Gholam A. Peyman Methods to regulate polarization and enhance function of cells
WO2007033180A1 (en) 2005-09-12 2007-03-22 Abela Pharmaceuticals, Inc. Materials for facilitating administration of dimethyl sulfoxide (dmso) and related compounds
US8480797B2 (en) 2005-09-12 2013-07-09 Abela Pharmaceuticals, Inc. Activated carbon systems for facilitating use of dimethyl sulfoxide (DMSO) by removal of same, related compounds, or associated odors
EP2324838A1 (en) 2005-09-12 2011-05-25 Abela Pharmaceuticals, Inc. Compositions Comprising Dimethyl Sulfoxide (DMSO)
WO2007033083A2 (en) 2005-09-12 2007-03-22 Abela Pharmaceuticals, Inc. Systems for removing dimethyl sulfoxide (dmso) or related compounds, or odors associated with same
JP2007106760A (ja) * 2005-09-16 2007-04-26 Kenji Yoshida 造血幹細胞増殖剤
MX342474B (es) 2005-09-23 2016-09-30 Cellerix Sl Poblaciones celulares con actividad inmunoreguladora, método de aislamiento y usos.
CA2999420A1 (en) 2005-09-27 2007-04-05 Tissuetech, Inc. Amniotic membrane preparations and purified compositions and methods of use
AU2006304277B2 (en) 2005-10-13 2012-07-05 Celularity Inc. Immunomodulation using placental stem cells
AU2006304274B2 (en) * 2005-10-13 2012-08-16 Celularity Inc. Production of oligodendrocytes from placenta-derived stem cells
JP4921767B2 (ja) * 2005-10-14 2012-04-25 株式会社カネカ 細胞の分化誘導方法
US10117900B2 (en) * 2005-11-09 2018-11-06 Athersys, Inc. MAPC treatment of brain injuries and diseases
WO2007056560A2 (en) * 2005-11-09 2007-05-18 Chemimage Corporation System and method for cytological analysis by raman spectroscopic imaging
US11000546B2 (en) 2005-11-09 2021-05-11 Athersys, Inc. Immunomodulatory properties of MAPCs and uses thereof
WO2007059084A2 (en) * 2005-11-14 2007-05-24 The New England Medical Center Hospitals, Inc. Methods for preparing cord matrix stem cells (cmsc) for long term storage and for preparing a segment of umbilical cord for cryopreservation
KR20080084808A (ko) * 2005-11-17 2008-09-19 바이오미메틱 세라퓨틱스, 인크. ｒｈＰＤＧＦ-ＢＢ 및 생체적합성 매트릭스를 사용하는상악안면골 보강
JP5289970B2 (ja) * 2005-12-16 2013-09-11 エシコン・インコーポレイテッド 組織適合性不適合な移植における逆免疫反応を抑制するための組成物および方法
AU2006327073B2 (en) * 2005-12-19 2012-08-30 Ethicon, Inc. In vitro expansion of postpartum derived cells in roller bottles
CA2634820C (en) * 2005-12-22 2021-05-25 Jane Ennis Viable cells from frozen umbilical cord tissue
US9125906B2 (en) 2005-12-28 2015-09-08 DePuy Synthes Products, Inc. Treatment of peripheral vascular disease using umbilical cord tissue-derived cells
CN105106239A (zh) * 2005-12-28 2015-12-02 伊西康公司 使用产后衍生细胞治疗外周血管疾病
EP1979050B1 (en) * 2005-12-28 2017-04-19 DePuy Synthes Products, Inc. Treatment of peripheral vascular disease using postpartum-derived cells
EP1974013A2 (en) * 2005-12-29 2008-10-01 Anthrogenesis Corporation Improved composition for collecting and preserving placental stem cells and methods of using the composition
US8455250B2 (en) 2005-12-29 2013-06-04 Anthrogenesis Corporation Co-culture of placental stem cells and stem cells from a second source
ES2671355T3 (es) 2005-12-29 2018-06-06 Anthrogenesis Corporation Poblaciones de células madre placentarias
EP1981970A4 (en) 2006-01-11 2009-10-21 Technion Res & Dev Foundation TISSUE CONSTRUCTION TISSUE TREATMENT DERIVED FROM A HUMAN EMBRYONIC STEM CELL
US20080286249A1 (en) * 2006-01-12 2008-11-20 Varney Timothy R Use of mesenchymal stem cells for treating genetic diseases and disorders
US20070253931A1 (en) * 2006-01-12 2007-11-01 Osiris Therapeutics, Inc. Use of mesenchymal stem cells for treating genetic diseases and disorders
US8871198B2 (en) * 2006-03-29 2014-10-28 Stemnion, Inc. Methods related to wound healing
US9944900B2 (en) * 2006-01-18 2018-04-17 Hemacell Perfusion Pulsatile perfusion extraction method for non-embryonic pluripotent stem cells
GB0600972D0 (en) * 2006-01-18 2006-03-01 Univ Leeds Enrichment of cells
US7875451B2 (en) * 2006-01-19 2011-01-25 The University Of Washington Formulation to improve survival of transplanted cells
CN103655618A (zh) 2006-01-23 2014-03-26 阿特西斯公司 无附加的免疫抑制治疗的mapc治疗法
CA2640185A1 (en) * 2006-01-24 2007-08-02 Christopher J. Centeno Mesenchymal stem cell isolation and transplantation method and system to be used in a clinical setting
FR2896511B1 (fr) * 2006-01-26 2012-10-26 Centre Nat Rech Scient Procede de culture de cellules issues du tissu adipeux et leurs applications.
ES2427993T3 (es) 2006-02-09 2013-11-05 Biomimetic Therapeutics, Llc Composiciones y métodos para el tratamiento de hueso
US20090232777A1 (en) * 2006-03-01 2009-09-17 Evy Lundgren-Akerlund Expansion and Differentiation of Mesenchymal Stem Cells
WO2007099534A2 (en) * 2006-03-01 2007-09-07 The Regenerative Medicine Institute Compostions and populations of cells obtained from the umbilical cord and methods of producing the same
ES2549528T3 (es) * 2006-03-23 2015-10-29 Pluristem Ltd. Métodos de expansión de células y usos de células y de medios de acondicionamiento producidos por los mismos para terapia
US20110171182A1 (en) * 2006-03-23 2011-07-14 Pluristem Ltd. Methods for cell expansion and uses of cells and conditioned media produced thereby for therapy
US7727763B2 (en) * 2006-04-17 2010-06-01 Bioe, Llc Differentiation of multi-lineage progenitor cells to respiratory epithelial cells
US20090208466A1 (en) * 2006-04-21 2009-08-20 James Yoo Ink-jet printing of tissues
US9456979B2 (en) * 2006-04-27 2016-10-04 Sri International Adminstration of intact mammalian cells to the brain by the intranasal route
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
EP2029149B1 (en) 2006-05-05 2017-04-19 John E. Davies Immune privileged and modulatory progenitor cells
EP4094578A1 (en) 2006-05-11 2022-11-30 Celularity Inc. Methods for collecting and using placenta cord blood stem cells
US20080050814A1 (en) * 2006-06-05 2008-02-28 Cryo-Cell International, Inc. Procurement, isolation and cryopreservation of fetal placental cells
WO2007146105A2 (en) * 2006-06-05 2007-12-21 Cryo-Cell International, Inc. Procurement, isolation and cryopreservation of fetal placental cells
WO2007146123A2 (en) * 2006-06-09 2007-12-21 Anthrogenesis Corporation Placental niche and use thereof to culture stem cells
US8475788B2 (en) 2006-06-14 2013-07-02 Stemnion, Inc. Methods of treating spinal cord injury and minimizing scarring
EP2035093A4 (en) * 2006-06-14 2010-02-17 Stemnion Inc METHODS FOR TREATING SPINAL CORD INJURY AND MINIMIZING THE SCALING SCALE
US20070292401A1 (en) * 2006-06-20 2007-12-20 Harmon Alexander M Soft tissue repair and regeneration using stem cell products
CN101627112A (zh) * 2006-06-28 2010-01-13 堪萨斯大学 将来自脐带基质的干细胞分化为肝细胞谱系细胞
US9161967B2 (en) 2006-06-30 2015-10-20 Biomimetic Therapeutics, Llc Compositions and methods for treating the vertebral column
US9642891B2 (en) * 2006-06-30 2017-05-09 Biomimetic Therapeutics, Llc Compositions and methods for treating rotator cuff injuries
EP2484308A1 (en) * 2006-07-24 2012-08-08 International Stem Cell Corporation Synthetic cornea from retinal stem cells
US7993918B2 (en) * 2006-08-04 2011-08-09 Anthrogenesis Corporation Tumor suppression using placental stem cells
US8822030B2 (en) 2006-08-11 2014-09-02 Aqua Resources Corporation Nanoplatelet metal hydroxides and methods of preparing same
WO2008021256A2 (en) * 2006-08-11 2008-02-21 Aqua Resources Corporation Nanoplatelet metal hydroxides and methods of preparing same
US7671014B2 (en) * 2006-08-14 2010-03-02 Warsaw Orthopedic, Inc. Flowable carrier matrix and methods for delivering to a patient
US8372437B2 (en) 2006-08-17 2013-02-12 Mimedx Group, Inc. Placental tissue grafts
US20100178274A1 (en) * 2006-08-22 2010-07-15 Ichiro Sekiya Application of synovium-derived mesenchymal stem cells (mscs) for cartilage or meniscus regeneration
US20080082170A1 (en) * 2006-09-29 2008-04-03 Peterman Marc M Apparatus and methods for surgical repair
US20080078411A1 (en) * 2006-10-03 2008-04-03 Restore Medical, Inc. Tongue implant for sleep apnea
US20080078412A1 (en) * 2006-10-03 2008-04-03 Restore Medical, Inc. Tongue implant
DK2078073T3 (da) 2006-10-12 2013-10-28 Ethicon Inc Nyreafledte celler og fremgangsmåder til anvendelse deraf til vævsreparation og -regenerering
EP1913869A3 (en) * 2006-10-19 2008-12-10 Esaote S.p.A. Diagnostic imaging method and apparatus for the anatomical region of the pelvic floor
ZA200902233B (en) * 2006-10-23 2010-07-28 Anthrogenesis Corp Methods and compositions for treatment of bone defects with placental cell populations
WO2008073628A2 (en) * 2006-11-03 2008-06-19 Biomimetic Therapeutics, Inc. Compositions and methods for arthrodetic procedures
EP2089511B1 (en) * 2006-11-13 2014-09-17 DePuy Synthes Products, LLC In vitro expansion of postpartum-derived cells using microcarriers
US20080132803A1 (en) * 2006-11-30 2008-06-05 Hyman Friedlander Method and system for doing business by mining the placental-chord complex
WO2008073331A2 (en) * 2006-12-07 2008-06-19 Teva Pharmaceutical Industries Ltd. Method of generation and expansion of tissue-progenitor cells and mature tissue cells from intact bone marrow or intact umbilical cord tissue
WO2008082525A1 (en) * 2006-12-19 2008-07-10 National Stem Cell Inc Umbilical cord stem cell secreted product derived topical compositions and methods of use thereof
US20100303770A1 (en) * 2006-12-28 2010-12-02 John Maslowski Methods for culturing dermal cells for treatment of skin injuries such as burns
US7980000B2 (en) 2006-12-29 2011-07-19 Applied Materials, Inc. Vapor dryer having hydrophilic end effector
ES2623141T3 (es) * 2007-01-17 2017-07-10 Noveome Biotherapeutics, Inc. Nuevos métodos para modular respuestas inflamatorias y/o inmunitarias
SI2120977T1 (sl) * 2007-02-12 2014-01-31 Anthrogenesis Coroporation Zdravljenje vnetnih bolezni z uporabo placentarnih matičnih celic
EP2129775A1 (en) * 2007-02-12 2009-12-09 Anthrogenesis Corporation Hepatocytes and chondrocytes from adherent placental stem cells; and cd34+, cd45- placental stem cell-enriched cell populations
CA2678875A1 (en) * 2007-02-20 2008-08-28 Biomimetic Therapeutics, Inc. Prevention and treatment for osteonecrosis and osteoradionecrosis of the jaw
CN102851254A (zh) * 2007-03-01 2013-01-02 干细胞国际公司 制备、分离及超低温保存子宫内膜/月经细胞
WO2008109816A1 (en) * 2007-03-08 2008-09-12 Hemacell Perfusion, Inc. Method for isolation of afterbirth derived cells
US20100172830A1 (en) * 2007-03-29 2010-07-08 Cellx Inc. Extraembryonic Tissue cells and method of use thereof
EP2626416A3 (en) * 2007-04-07 2013-12-18 The Whitehead Institute for Biomedical Research Reprogramming of somatic cells
EP2139497B1 (en) * 2007-04-13 2013-11-06 Stemnion, INC. Methods for treating nervous system injury and disease
CN101668848B (zh) 2007-04-26 2014-10-22 雷蒙特亚特特拉维夫大学有限公司 来自口腔粘膜的多能自体干细胞和使用方法
WO2008137115A1 (en) 2007-05-03 2008-11-13 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
US8574567B2 (en) 2007-05-03 2013-11-05 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
US8114668B2 (en) * 2007-05-14 2012-02-14 Cardiac Pacemakers, Inc. Composition for cold storage of stem cells
KR20150041157A (ko) 2007-05-21 2015-04-15 웨이크 포리스트 유니버시티 헬스 사이언시즈 소변으로부터의 전구 세포 및 이를 사용하는 방법
TWM322542U (en) * 2007-05-23 2007-11-21 Universal Scient Ind Co Ltd Testing machine
US20090053182A1 (en) * 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
DE602007010434D1 (de) * 2007-06-01 2010-12-23 Allergan Inc Gerät zur Erzeugung des zugspannungsinduzierten Wachstums von biologischem Gewebe
US9693486B1 (en) * 2007-06-14 2017-06-27 Switch, Ltd. Air handling unit with a canopy thereover for use with a data center and method of using the same
US8273526B2 (en) 2007-06-18 2012-09-25 Children's Hospital & Research Center At Oakland Method of isolating stem and progenitor cells from placenta
US20090004253A1 (en) * 2007-06-29 2009-01-01 Brown Laura J Composite device for the repair or regeneration of tissue
US20090004271A1 (en) 2007-06-29 2009-01-01 Brown Laura J Morselized foam for wound treatment
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
US9095562B2 (en) 2007-07-05 2015-08-04 Regenerative Sciences, Inc. Methods and compositions for optimized expansion and implantation of mesenchymal stem cells
DE102007034679A1 (de) * 2007-07-25 2009-01-29 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Materialzusammensetzungen, welche aus exokrinem Drüsengewebe erhaltene adulte Stammzellen enthalten, insbesondere zur Verwendung in der Regenerationsmedizin, z.B. zur Wiederherstellung von verletztem oder geschädigtem Myokardgewebe
US20090024224A1 (en) 2007-07-16 2009-01-22 Chen Silvia S Implantation of cartilage
EP2192908A4 (en) * 2007-07-25 2010-09-01 Bioe Inc DIFFERENTIATION OF PRECURSOR CELLS OF MULTIPLE ABSTRACTS AGAINST CHONDROCYTES
EP2520309A1 (en) 2007-07-27 2012-11-07 Humacyte, Inc. Compositions and methods for soft tissue augmentation
KR101617243B1 (ko) 2007-07-31 2016-05-02 라이프스캔, 인코포레이티드 인간 배아 줄기 세포의 분화
AU2008284277B2 (en) * 2007-08-08 2011-04-28 Shire Regenerative Medicine, Inc. Materials and methods for treating skeletal system damage and promoting skeletal system repair and regeneration
US20090062907A1 (en) * 2007-08-31 2009-03-05 Quijano Rodolfo C Self-expanding valve for the venous system
US20090068153A1 (en) * 2007-09-06 2009-03-12 Vitelli Francesca P Cell composition for tissue regeneration
EP2197270B8 (en) 2007-09-07 2020-03-04 Surgical Biologics, LLC Placental tissue grafts and improved methods of preparing and using the same
KR20140107677A (ko) 2007-09-19 2014-09-04 플루리스템 리미티드 지방 또는 태반 조직 유래의 부착 세포 및 이의 치료 용도
US20090136553A1 (en) * 2007-09-25 2009-05-28 Gerlach Jorg C Triggerably dissolvable hollow fibers for controlled delivery
KR20220122774A (ko) * 2007-09-26 2022-09-02 셀룰래리티 인코포레이티드 인간 태반 관류액으로부터의 혈관형성 세포
RS53841B1 (sr) 2007-09-28 2015-06-30 Anthrogenesis Corporation Supresija tumora korišćenjem humanog placentalnog perfuzata i intermedijernih ćelija ubica poreklom iz humane placente
CA2701435C (en) * 2007-10-05 2017-06-20 Ethicon, Inc. Repair and regeneration of renal tissue using human umbilical cord tissue-derived cells
WO2009052209A2 (en) * 2007-10-16 2009-04-23 University Of Kansas Isolation of stem cells and effective control of contamination
US20100297234A1 (en) * 2007-10-19 2010-11-25 Ilene Sugino Method of using an extracellular matrix to enhance cell transplant survival and differentiation
US20120219737A1 (en) 2007-10-19 2012-08-30 University Of Medicine And Dentistry Of New Jersey Production of extracellular matrix, conditioned media and uses thereof
DK2217329T3 (en) * 2007-11-07 2018-04-23 Anthrogenesis Corp APPLICATION OF NAVLINE BLOOD IN THE TREATMENT OF COMPLIANCES IN CONNECTION WITH PREGNANCY BIRTH
WO2009064958A1 (en) * 2007-11-14 2009-05-22 Osteosphere, Llc Ex-vivo production of human demineralized bone matrix
CA3123528C (en) 2007-11-27 2026-01-13 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic cells
US20090163990A1 (en) * 2007-12-19 2009-06-25 Chunlin Yang Decellularized omentum matrix and uses thereof
US20110200642A1 (en) * 2007-12-19 2011-08-18 Regenerative Sciences, Llc Compositions and Methods to Promote Implantation and Engrafment of Stem Cells
US8236538B2 (en) 2007-12-20 2012-08-07 Advanced Technologies And Regenerative Medicine, Llc Methods for sterilizing materials containing biologically active agents
WO2009085216A2 (en) 2007-12-20 2009-07-09 Squicor Compositions and methods for detecting or elimninating senescent cells to diagnose or treat disease
ES2621610T3 (es) * 2007-12-27 2017-07-04 DePuy Synthes Products, Inc. Tratamiento de la degeneración de discos intervertebrales utilizando células derivadas de tejido cordón umbilical humano
US20100279413A1 (en) * 2008-01-14 2010-11-04 Zymes, Llc Applications of ubiquinones and ubiquinols
US8088163B1 (en) 2008-02-06 2012-01-03 Kleiner Jeffrey B Tools and methods for spinal fusion
AU2009212151C1 (en) 2008-02-07 2015-09-17 Stryker Corporation Compositions and methods for distraction osteogenesis
EP2254608B1 (en) * 2008-02-07 2016-05-04 Shahar Cohen Compartmental extract compositions for tissue engineering
US20090209456A1 (en) * 2008-02-19 2009-08-20 Iliana Sweis Compositions and methods for improving facial and body aesthetics
BR122017025207B1 (pt) 2008-02-21 2021-03-16 Centocor Ortho Biotech Inc superfície que faz parte de um recipiente ou matriz destinada para uso em uma cultura de células ou análises, desprovida de uma camada de células alimentadoras e desprovida de uma camada adsorvente
US8318485B2 (en) * 2008-02-25 2012-11-27 Natalie Gavrilova Stem cell therapy for the treatment of diabetic retinopathy and diabetic optic neuropathy
US20090220995A1 (en) * 2008-02-28 2009-09-03 Sachs David H Multiple administrations of umbilicus derived cells
US20090232782A1 (en) * 2008-03-14 2009-09-17 Yu-Show Fu Method for treating brain ischemic injury through transplantation of human umbilical mesenchymal stem cells
US20110052533A1 (en) * 2008-03-14 2011-03-03 Regenerative Sciences, Llc Compositions and Methods for Cartilage Repair
AU2009240738B2 (en) 2008-04-21 2014-09-11 Tissue Regeneration Therapeutics, Inc. Genetically modified human umbilical cord perivascular cells for prophylaxis against or treatment of biological or chemical agents
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
US9358320B2 (en) 2008-04-25 2016-06-07 Allosource Multi-layer tissue patches
US9480549B2 (en) 2008-04-25 2016-11-01 Allosource Multi-layer tissue patches
WO2009134991A2 (en) 2008-04-30 2009-11-05 Ethicon, Inc. Tissue engineered blood vessel
US20110143429A1 (en) * 2008-04-30 2011-06-16 Iksoo Chun Tissue engineered blood vessels
CA2724839A1 (en) * 2008-05-21 2009-11-26 Bioe Llc Differentiation of multi-lineage progenitor cells to pancreatic cells
US20210378834A1 (en) 2008-05-22 2021-12-09 Spinal Surgical Strategies, Inc., A Nevada Corporation D/B/A Kleiner Device Labs Spinal fusion cage system with inserter
WO2010011407A2 (en) * 2008-05-23 2010-01-28 President And Fellows Of Harvard College Methods of generating patterned soft substrates and uses thereof
US20090297485A1 (en) * 2008-05-28 2009-12-03 Allan Mishra Compositions and methods for treating psychiatric and neurodegenerative disorders
CA2727625A1 (en) * 2008-06-11 2009-12-17 The Children's Mercy Hospital Composition comprising decellularized tissue
AU2009257219B2 (en) 2008-06-13 2015-01-29 Whitehead Institute For Biomedical Research Programming and reprogramming of cells
MX2010014304A (es) * 2008-06-26 2011-02-15 Kci Licensing Inc Estimulacion de la formacion de cartilago utilizando el tratamiento de presion reducida.
WO2009157559A1 (ja) * 2008-06-27 2009-12-30 独立行政法人産業技術総合研究所 膵臓疾患又は糖尿病のための膵臓細胞再生移植用キット
EP2318516A1 (en) * 2008-06-30 2011-05-11 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
US20100015711A1 (en) 2008-06-30 2010-01-21 Janet Davis Differentiation of Pluripotent Stem Cells
US20100028307A1 (en) * 2008-07-31 2010-02-04 O'neil John J Pluripotent stem cell differentiation
DK2330889T3 (en) 2008-08-20 2017-01-30 Anthrogenesis Corp Improved cell composition and process for making the same
KR20180108887A (ko) * 2008-08-20 2018-10-04 안트로제네시스 코포레이션 단리된 태반 세포를 사용한 뇌졸중 치료
MX2011001992A (es) * 2008-08-22 2011-03-29 Anthrogenesis Corp Metodos y composiciones para el tratamiento de defectos oseos con poblaciones de celulas placentarias.
MX2011002328A (es) 2008-09-02 2011-05-19 Pluristem Ltd Celulas adherentes del tejido de placenta y su uso en terapia.
MX2011002555A (es) 2008-09-09 2011-08-03 Biomimetic Therapeutics Inc Composiciones de factor de crecimiento derivadas de plaquetas y metodo para el tratamiento de lesiones de tendon y ligamentos.
US9446227B2 (en) 2008-09-12 2016-09-20 Sonescence, Inc. Ultrasonic dispersion of compositions in tissue
US20100069827A1 (en) * 2008-09-12 2010-03-18 Barry Neil Silberg Pre-Surgical Prophylactic Administration of Antibiotics and Therapeutic Agents
ES2694000T3 (es) 2008-10-06 2018-12-17 3-D Matrix Ltd. Tapón de tejido
WO2010048418A1 (en) * 2008-10-22 2010-04-29 The Trustees Of Columbia University In The City Of New York Cartilage regeneration without cell transplantation
CA2742267C (en) 2008-10-31 2019-06-04 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
US20100119492A1 (en) 2008-10-31 2010-05-13 Synthes Usa, Llc Method and device for activating stem cells
BRPI0919885A2 (pt) * 2008-10-31 2015-08-11 Centocor Ortho Biotech Inc Diferenciação de células-tronco embrionárias humanas para a linhagem endócrina pancreática
BRPI0921494A2 (pt) 2008-11-03 2018-10-30 Prad Reasearch And Development Ltd método de planejamento de uma operação de amostragem para uma formação subterrãnea, método de contolar uma operação de amostragem de formação subterrânea, método de controlar uma operação de perfuração para uma formação subterrãnea, e método de realizar uma amostragem durante a operação de perfuração.
CA2743566C (en) * 2008-11-19 2021-11-09 Anthrogenesis Corporation Amnion derived adherent cells
AU2009316583B2 (en) * 2008-11-20 2016-04-21 Janssen Biotech, Inc. Methods and compositions for cell attachment and cultivation on planar substrates
US20100124781A1 (en) 2008-11-20 2010-05-20 Shelley Nelson Pluripotent Stem Cell Culture on Micro-Carriers
RU2011116449A (ru) * 2008-11-20 2012-12-27 Лайфселл Корпорейшн Способ лечения и предотвращения парастомальных грыж
NZ602569A (en) * 2008-11-21 2014-03-28 Anthrogenesis Corp Treatment of diseases, disorders or conditions of the lung using placental cells
US8366748B2 (en) 2008-12-05 2013-02-05 Kleiner Jeffrey Apparatus and method of spinal implant and fusion
WO2010065239A1 (en) * 2008-12-05 2010-06-10 Wake Forest University Health Sciences Stem cells from urine and methods for using the same
WO2010064702A1 (ja) * 2008-12-05 2010-06-10 国立大学法人 東京大学 癌の予後を予測するためのバイオマーカー
JP2012510874A (ja) 2008-12-05 2012-05-17 リジェネレイティブ サイエンシーズ， エルエルシー 無血管組織の修復を促進するための方法及び組成物
US20100168022A1 (en) * 2008-12-11 2010-07-01 Centeno Christopher J Use of In-Vitro Culture to Design or Test Personalized Treatment Regimens
JP5646502B2 (ja) * 2008-12-19 2014-12-24 デピュイ・シンセス・プロダクツ・エルエルシーＤｅＰｕｙ Ｓｙｎｔｈｅｓ Ｐｒｏｄｕｃｔｓ， ＬＬＣ 神経因性疼痛を治療するための臍帯組織由来細胞
AU2009327383B2 (en) * 2008-12-19 2014-08-28 DePuy Synthes Products, LLC Regeneration and repair of neural tissue following injury
US10179900B2 (en) * 2008-12-19 2019-01-15 DePuy Synthes Products, Inc. Conditioned media and methods of making a conditioned media
CN107028983A (zh) * 2008-12-19 2017-08-11 德普伊新特斯产品有限责任公司 肺部疾病和病症的治疗
US20130302283A1 (en) * 2012-05-14 2013-11-14 Advanced Technologies And Regenerative Medicine, Llc hUTC MODULATION OF PRO-INFLAMMATORY MEDIATORS OF LUNG AND PULMONARY DISEASES AND DISORDERS
US8771677B2 (en) * 2008-12-29 2014-07-08 Vladimir B Serikov Colony-forming unit cell of human chorion and method to obtain and use thereof
KR20110107357A (ko) * 2008-12-30 2011-09-30 케이씨아이 라이센싱 인코포레이티드 연골 회복을 위한 미세골절 수술의 감압 증대
JP5701614B2 (ja) * 2009-01-23 2015-04-15 国立大学法人 東京大学 培養細胞の評価方法
US9247943B1 (en) 2009-02-06 2016-02-02 Kleiner Intellectual Property, Llc Devices and methods for preparing an intervertebral workspace
WO2010093468A1 (en) * 2009-02-12 2010-08-19 University Of Southern California Bioadhesive patch for sutureless closure of soft tissue
KR101422690B1 (ko) * 2009-02-27 2014-07-23 (주)차바이오앤디오스텍 배아줄기세포 유래 혈관형성전구세포의 배양 분비물을 포함하는 피부재생용 조성물 및 이의 용도
US8722034B2 (en) * 2009-03-26 2014-05-13 Depuy Synthes Products Llc hUTC as therapy for Alzheimer's disease
CN102439136A (zh) * 2009-03-26 2012-05-02 加州大学校董 产生用于疾病修饰的抑制性rna的间充质干细胞
WO2010118059A1 (en) * 2009-04-06 2010-10-14 Capricor, Inc. Systems and methods for cardiac tissue repair
MX357440B (es) * 2009-05-13 2018-07-10 Medipost Co Ltd Trombospondina 1 (tsp-1), trombospondina 2 (tsp-2), receptor de interleucina 17b (il-17br) y factor de crecimiento tipo factor de crecimiento epidermico de union a heparina (hb-egf) asociados con actividades de celulas madre y aplicaciones de los mismos.
CA2762212C (en) * 2009-05-20 2015-12-29 Humacyte, Inc. Elastin for soft tissue augmentation
WO2010138782A1 (en) * 2009-05-28 2010-12-02 University Of Central Florida Research In vitro production of oligodendrocytes from human umbilical cord stem cells
US20150335400A1 (en) * 2009-06-17 2015-11-26 The Trustees Of Columbia University In The City Of New York Tooth scaffolds
US8647617B2 (en) 2009-07-13 2014-02-11 Stemnion, Inc. Methods for modulating inflammatory and/or immune responses
CA2768644A1 (en) 2009-07-20 2011-01-27 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
BR112012001480A2 (pt) 2009-07-20 2015-09-01 Janssen Biotech Inc Diferenciação de células-tronco embriônicas humanas
JP5819826B2 (ja) * 2009-07-20 2015-11-24 ヤンセン バイオテツク，インコーポレーテツド ヒト胚性幹細胞の分化
AU2010276201B2 (en) * 2009-07-21 2013-10-17 Healios K.K. Use of stem cells to reduce leukocyte extravasation
ES2360434B1 (es) * 2009-07-21 2012-04-12 Universitat Internacional De Catalunya Celulas madre pluripotenciales obtenidas a partir de la pulpa dental.
WO2011022071A2 (en) * 2009-08-20 2011-02-24 The Regents Of The University Of California Cardiac compositions
US20110054929A1 (en) * 2009-09-01 2011-03-03 Cell Solutions Colorado Llc Stem Cell Marketplace
US8207651B2 (en) 2009-09-16 2012-06-26 Tyco Healthcare Group Lp Low energy or minimum disturbance method for measuring frequency response functions of ultrasonic surgical devices in determining optimum operating point
USD723682S1 (en) 2013-05-03 2015-03-03 Spinal Surgical Strategies, Llc Bone graft delivery tool
US8685031B2 (en) 2009-09-18 2014-04-01 Spinal Surgical Strategies, Llc Bone graft delivery system
USD750249S1 (en) 2014-10-20 2016-02-23 Spinal Surgical Strategies, Llc Expandable fusion cage
US8906028B2 (en) 2009-09-18 2014-12-09 Spinal Surgical Strategies, Llc Bone graft delivery device and method of using the same
US20170238984A1 (en) 2009-09-18 2017-08-24 Spinal Surgical Strategies, Llc Bone graft delivery device with positioning handle
US9060877B2 (en) 2009-09-18 2015-06-23 Spinal Surgical Strategies, Llc Fusion cage with combined biological delivery system
US10973656B2 (en) 2009-09-18 2021-04-13 Spinal Surgical Strategies, Inc. Bone graft delivery system and method for using same
US10245159B1 (en) 2009-09-18 2019-04-02 Spinal Surgical Strategies, Llc Bone graft delivery system and method for using same
US9186193B2 (en) 2009-09-18 2015-11-17 Spinal Surgical Strategies, Llc Fusion cage with combined biological delivery system
US9173694B2 (en) 2009-09-18 2015-11-03 Spinal Surgical Strategies, Llc Fusion cage with combined biological delivery system
US9629729B2 (en) 2009-09-18 2017-04-25 Spinal Surgical Strategies, Llc Biological delivery system with adaptable fusion cage interface
WO2011038133A2 (en) * 2009-09-23 2011-03-31 Davinci Biosciences Llc Umbilical cord lining stem cells and methods and material for isolating and culturing same
CA2777663A1 (en) 2009-10-16 2011-04-21 University Of Medicine And Dentistry Of New Jersey Method for treating chronic nerve tissue injury using a cell therapy strategy
ES2665006T3 (es) * 2009-10-29 2018-04-24 Janssen Biotech, Inc. Células madre pluripotentes
US9839609B2 (en) 2009-10-30 2017-12-12 Abela Pharmaceuticals, Inc. Dimethyl sulfoxide (DMSO) and methylsulfonylmethane (MSM) formulations to treat osteoarthritis
US9113950B2 (en) 2009-11-04 2015-08-25 Regenerative Sciences, Llc Therapeutic delivery device
US20130028978A1 (en) * 2009-11-10 2013-01-31 The Trustees Of Columbia University In The City Of New York Compositions and methods for wound treatment
US20130129686A1 (en) * 2009-11-19 2013-05-23 Regents Of The University Reducing Inflammation Using Cell Therapy
CA2784415C (en) 2009-12-23 2019-06-18 Jean Xu Differentiation of human embryonic stem cells
US9133439B2 (en) * 2009-12-23 2015-09-15 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
WO2011094181A1 (en) * 2010-01-26 2011-08-04 Anthrogenesis Corporation Treatment of bone-related cancers using placental stem cells
NZ601400A (en) 2010-01-26 2013-11-29 Stem Cells Spin S A Cell homogenate from stem cells derived from growing deer antlers, a method of obtaining it and its use
US20110212065A1 (en) 2010-02-18 2011-09-01 Timothy Jansen Methods of manufacture of therapeutic products comprising vitalized placental dispersions
EP3769695A1 (en) * 2010-02-19 2021-01-27 LifeCell Corporation Abdominal wall treatment devices
WO2011103598A1 (en) 2010-02-22 2011-08-25 Biomimetic Therapeutics, Inc. Platelet-derived growth factor compositions and methods for the treatment of tendinopathies
WO2011109279A2 (en) 2010-03-01 2011-09-09 Centocor Ortho Biotech Inc. Methods for purifying cells derived from pluripotent stem cells
SI2556145T1 (sl) 2010-04-07 2017-01-31 Anthrogenesis Corporation Angiogeneza z uporabo placentnih matičnih celic
AU2011237743A1 (en) 2010-04-08 2012-11-01 Anthrogenesis Corporation Treatment of sarcoidosis using placental stem cells
US9845457B2 (en) 2010-04-30 2017-12-19 Cedars-Sinai Medical Center Maintenance of genomic stability in cultured stem cells
US8529883B2 (en) 2010-05-07 2013-09-10 Fibrocell Technologies, Inc. Dosage unit formulations of autologous dermal fibroblasts
US9752125B2 (en) 2010-05-12 2017-09-05 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
US9352003B1 (en) 2010-05-14 2016-05-31 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US8883210B1 (en) 2010-05-14 2014-11-11 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US10130736B1 (en) 2010-05-14 2018-11-20 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
WO2011153236A1 (en) * 2010-06-03 2011-12-08 The Board Of Trustees Of The Leland Stanford Junior University Purified compositions of cardiovascular progenitor cells
ES2879246T3 (es) 2010-07-02 2021-11-22 Univ North Carolina Chapel Hill Andamios de biomatriz
AU2011279250B2 (en) 2010-07-12 2014-12-04 California Institute Of Technology Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same
CN107828727A (zh) 2010-07-13 2018-03-23 人类起源公司 产生自然杀伤细胞的方法、由此获得的细胞群体及其用途
US8825388B2 (en) 2010-07-13 2014-09-02 Qualcomm Incorporated Indoor likelihood heatmap
EP2600913A4 (en) * 2010-08-03 2015-02-18 Agency Science Tech & Res MICROFABRICATED SUPPORT STRUCTURES
JP2012031127A (ja) * 2010-08-03 2012-02-16 Nagoya Univ 臍帯由来間葉系幹細胞を含む組成物
IL207586A0 (en) 2010-08-12 2010-12-30 Omrix Biopharmaceuticals Ltd A fibrin based therapeutic preparation and use thereof
WO2012021885A1 (en) * 2010-08-13 2012-02-16 The Trustees Of Columbia University In The City Of New York Three-dimensional tissue engineering devices and uses thereof
CA2809300A1 (en) 2010-08-31 2012-03-08 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
CN108517310B (zh) 2010-08-31 2022-02-15 詹森生物科技公司 人胚胎干细胞的分化
PH12013500349A1 (en) 2010-08-31 2022-03-30 Janssen Biotech Inc Differentiation of pluripotent stem cells
CN103180435A (zh) * 2010-08-31 2013-06-26 库克通用生物技术有限责任公司 用于治疗动物疾病的全身的同种异体干细胞疗法
WO2012048275A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US8546338B2 (en) 2010-12-08 2013-10-01 Johnson & Johnson Consumer Companies, Inc. Self-assembling hydrogels based on dicephalic peptide amphiphiles
US8574899B2 (en) 2010-12-22 2013-11-05 Vladimir B Serikov Methods for augmentation collection of placental hematopoietic stem cells and uses thereof
AR093183A1 (es) 2010-12-31 2015-05-27 Anthrogenesis Corp Aumento de la potencia de celulas madre de placenta usando moleculas de arn moduladoras
JP5388233B2 (ja) * 2011-01-19 2014-01-15 富士ソフト株式会社 再生軟骨の軟骨特性を評価する方法
US12093036B2 (en) 2011-01-21 2024-09-17 Teladoc Health, Inc. Telerobotic system with a dual application screen presentation
US8945536B2 (en) * 2011-01-26 2015-02-03 The Chinese University Of Hong Kong Stem cell sheet for tissue repair
EP2686419B1 (en) 2011-03-18 2016-11-16 MicroVascular Tissues, Inc. Allogeneic microvascular tissue for soft tissue treatments
EP3260533B1 (en) 2011-03-22 2019-09-11 Pluristem Ltd. Methods for treating radiation or chemical injury
WO2012149486A1 (en) 2011-04-28 2012-11-01 Tissuetech, Inc. Methods of modulating bone remodeling
US9758395B2 (en) 2011-04-28 2017-09-12 Aquero Company, Llc Lysine-based polymer coagulants for use in clarification of process waters
WO2012149468A2 (en) 2011-04-29 2012-11-01 University Of Southern California Instruments and methods for the implantation of cell-seeded substrates
US8877489B2 (en) 2011-12-05 2014-11-04 California Institute Of Technology Ultrathin parylene-C semipermeable membranes for biomedical applications
US8834928B1 (en) 2011-05-16 2014-09-16 Musculoskeletal Transplant Foundation Tissue-derived tissugenic implants, and methods of fabricating and using same
AU2012262311B2 (en) * 2011-05-31 2016-04-28 Lifecell Corporation Adipose tissue matrices
EP3443968A1 (en) 2011-06-01 2019-02-20 Celularity, Inc. Treatment of pain using placental stem cells
ES2822301T3 (es) 2011-06-10 2021-04-30 Tissuetech Inc Métodos de procesamiento de tejidos de soporte fetal
US20140189897A1 (en) 2011-06-21 2014-07-03 Mayo Foundation For Medical Education And Research Transgenic animals capable of being induced to delete senescent cells
US9133438B2 (en) 2011-06-29 2015-09-15 Biorestorative Therapies, Inc. Brown fat cell compositions and methods
US20130005829A1 (en) 2011-06-30 2013-01-03 Advanced Technologies And Regenerative Medicine, Llc. Segmented, epsilon-Caprolactone-Rich, Poly(epsilon-Caprolactone-co-p-Dioxanone) Copolymers for Medical Applications and Devices Therefrom
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
ES2661587T3 (es) 2011-08-10 2018-04-02 Depuy Synthes Products Llc Tratamiento de enfermedad vascular periférica usando células derivadas de tejido del cordón umbilical
WO2013055476A1 (en) 2011-09-09 2013-04-18 Anthrogenesis Corporation Treatment of amyotrophic lateral sclerosis using placental stem cells
US9248013B2 (en) 2011-12-05 2016-02-02 California Institute Of Technology 3-Dimensional parylene scaffold cage
WO2013090645A1 (en) 2011-12-13 2013-06-20 Buck Institute For Research On Aging Methods for improving medical therapies
US9162011B2 (en) 2011-12-19 2015-10-20 Allosource Flowable matrix compositions and methods
US20130157365A1 (en) * 2011-12-20 2013-06-20 Advanced Technologies And Regenerative Medicine, Llc Induced pluripotent stem cells from human umbilical cord tissue-derived cells
AU2012355463C1 (en) 2011-12-20 2016-09-22 Lifecell Corporation Sheet tissue products
WO2013096252A1 (en) 2011-12-20 2013-06-27 Lifecell Corporation Flowable tissue products
KR102203056B1 (ko) 2011-12-22 2021-01-14 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 단일 인슐린 호르몬 양성 세포로의 분화
JP6301263B2 (ja) 2011-12-23 2018-03-28 デピュイ・シンセス・プロダクツ・インコーポレイテッド ヒト臍帯組織由来細胞の検出
SI3321355T1 (sl) 2011-12-30 2021-11-30 Amit Patel Postopki in sestavki za klinično izpeljavo alogenske celice in terapevtske uporabe
DK2806907T3 (en) 2012-01-24 2019-02-18 Lifecell Corp EXTENDED TISSUE MATRIX
US9575054B2 (en) * 2012-02-23 2017-02-21 Anthrogenesis Corporation Identification of antitumor compounds using placenta
US8940294B2 (en) 2012-03-02 2015-01-27 Tissuetech, Inc. Methods of isolating and culturing stem cells
KR20140131999A (ko) 2012-03-07 2014-11-14 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 증폭 및 유지를 위한 한정 배지
US20150064137A1 (en) 2012-04-17 2015-03-05 Kythera Biopharmaceuticals, Inc. Use of engineered viruses to specifically kill senescent cells
US9999637B2 (en) 2012-04-24 2018-06-19 Lifecell Corporation Functionalized tissue matrices
US9884076B2 (en) 2012-06-05 2018-02-06 Capricor, Inc. Optimized methods for generation of cardiac stem cells from cardiac tissue and their use in cardiac therapy
KR102667288B1 (ko) 2012-06-08 2024-05-17 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 췌장 내분비 세포로의 분화
ES2706065T3 (es) 2012-07-06 2019-03-27 3 D Matrix Ltd Proceso de llenado-acabado para soluciones de péptidos
JP6282270B2 (ja) 2012-07-09 2018-02-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 組み換えにより生成されたパエニバシラス・ポリミキサに由来する中性プロテアーゼ
US11090338B2 (en) 2012-07-13 2021-08-17 Lifecell Corporation Methods for improved treatment of adipose tissue
US8960674B2 (en) 2012-07-27 2015-02-24 Bally Gaming, Inc. Batch card shuffling apparatuses including multi-card storage compartments, and related methods
AU2013302799B2 (en) 2012-08-13 2018-03-01 Cedars-Sinai Medical Center Exosomes and micro-ribonucleic acids for tissue regeneration
US9901080B2 (en) 2012-08-23 2018-02-27 Buck Institute For Research On Aging Transgenic mouse having a transgene that converts a prodrug into a cytotoxic compound in senescent cells
US9901081B2 (en) 2012-08-23 2018-02-27 Buck Institute For Research On Aging Transgenic mouse for determining the role of senescent cells in cancer
US9872937B2 (en) 2012-09-19 2018-01-23 Microvascular Tissues, Inc. Compositions and methods for treating and preventing tissue injury and disease
US10596202B2 (en) 2012-09-19 2020-03-24 Microvascular Tissues, Inc. Compositions and methods for treating and preventing tissue injury and disease
ES2710921T3 (es) 2012-09-19 2019-04-29 Microvascular Tissues Inc Composiciones para tratar y prevenir lesión y enfermedad tisular
US11819522B2 (en) 2012-09-19 2023-11-21 Microvascular Tissues, Inc. Compositions and methods for treating and preventing tissue injury and disease
CA2885327A1 (en) 2012-09-26 2014-04-03 Lifecell Corporation Processed adipose tissue
KR101728808B1 (ko) * 2012-09-28 2017-04-20 한국생명공학연구원 아세카이니드 또는 이의 유도체를 포함하는 근력약화 관련 질환의 예방 또는 치료용 약학적 조성물
ITTO20120859A1 (it) * 2012-10-02 2014-04-03 Univ Degli Studi Torino Nuova applicazione terapeutica di un mezzo condizionato da cellule staminali mesenchimali placentari
WO2014066649A1 (en) * 2012-10-26 2014-05-01 The Regents Of The University Of California A strategy for engineering various 3d tissues, organoids and vasculature
US10279018B2 (en) 2012-12-03 2019-05-07 Unity Biotechnology, Inc. Immunogenic compositions for inducing an immune response for elimination of senescent cells
US20140170748A1 (en) 2012-12-14 2014-06-19 DePuy Synthes Products, LLC Nutrient Enriched Media for hUTC Growth
WO2014105546A1 (en) 2012-12-31 2014-07-03 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators
RU2768963C2 (ru) 2012-12-31 2022-03-25 Янссен Байотек, Инк. Культивация эмбриональных стволовых клеток человека в воздушно-жидкостной зоне взаимодействия с целью их дифференцировки в панкреатические эндокринные клетки
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
WO2014106141A1 (en) 2012-12-31 2014-07-03 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
EP2756754B1 (de) 2013-01-17 2017-01-04 Vita 34 Ag Verfahren zur Behandlung von Nabelschnurgewebe, insbesondere im Zusammenhang mit der Konservierung des Gewebes
US20140212390A1 (en) * 2013-01-30 2014-07-31 NuTech Medical, Inc. Placental Membrane Preparation and Methods of Making and Using Same
AU2014215458A1 (en) 2013-02-05 2015-08-13 Anthrogenesis Corporation Natural killer cells from placenta
WO2014164815A2 (en) 2013-03-12 2014-10-09 Allergan, Inc. Adipose tissue combinations, devices, and uses thereof
CA2899246C (en) 2013-03-13 2022-12-06 Allosource Fascia fibrous compositions and methods for their use and manufacture
EP3882336A1 (en) * 2013-03-14 2021-09-22 Celularity Inc. Enhanced placental stem cells and uses thereof
WO2014150784A1 (en) 2013-03-15 2014-09-25 Allosource Cell repopulated collagen matrix for soft tissue repair and regeneration
RU2539750C2 (ru) * 2013-04-09 2015-01-27 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) Способ оценки иммуносупрессивных свойств мезенхимальных стромальных клеток человека
US20140350516A1 (en) 2013-05-23 2014-11-27 Allergan, Inc. Mechanical syringe accessory
US11229789B2 (en) 2013-05-30 2022-01-25 Neurostim Oab, Inc. Neuro activator with controller
JP2016523125A (ja) 2013-05-30 2016-08-08 グラハム エイチ． クリーシー 局所神経性刺激
US10058572B2 (en) * 2013-08-15 2018-08-28 The Regents Of The University Of California Placenta-derived multipotent stem cells
US9248384B2 (en) 2013-10-02 2016-02-02 Allergan, Inc. Fat processing system
CN105793411B (zh) 2013-11-16 2018-04-17 泰尔茂比司特公司 生物反应器中的细胞扩增
EP3076982B1 (en) 2013-12-06 2020-02-19 Allosource Method of drying sheets of tissue
WO2015095380A1 (en) * 2013-12-17 2015-06-25 Kassab Ghassan S Devices, systems and methods for tissue engineering of luminal grafts
US20190269675A1 (en) 2014-01-28 2019-09-05 Buck Institute for Research and Aging Treatment of parkinson's disease and other conditions caused or mediated by senescent astrocytes using small molecule senolytic agents
US20170216286A1 (en) 2014-01-28 2017-08-03 Mayo Foundation For Medical Education And Research Killing senescent cells and treating senescence-associated conditions using a src inhibitor and a flavonoid
EP3099380B1 (en) 2014-01-28 2021-08-11 Buck Institute for Research on Aging Methods and compositions for killing senescent cells and for treating senescence-associated diseases and disorders
US10328058B2 (en) 2014-01-28 2019-06-25 Mayo Foundation For Medical Education And Research Treating atherosclerosis by removing senescent foam cell macrophages from atherosclerotic plaques
WO2016179043A1 (en) * 2015-05-01 2016-11-10 Dermtech, Inc. Non-invasive skin collection system
EP4555981A3 (en) 2014-02-12 2025-08-06 Genentech, Inc. Method and apparatus for suprachoroidal administration of therapeutic agent
BR112016020584A2 (pt) 2014-03-10 2017-10-03 3 D Matrix Ltd Composições de peptídeo auto-organizável
JP6684719B2 (ja) 2014-03-10 2020-04-22 株式会社スリー・ディー・マトリックス ペプチド組成物の滅菌および濾過
JP6783143B2 (ja) 2014-03-25 2020-11-11 テルモ ビーシーティー、インコーポレーテッド 培地の受動的補充
CA2948126A1 (en) * 2014-05-07 2015-11-12 Osiris Therapeutics, Inc. Therapeutic placental compositions, methods of making and methods of use
EP3140417B1 (en) 2014-05-09 2021-04-21 Reelabs Private Limited Foetal polymix of mesenchymal stem cells under hypoxic conditions for the treatment of clinical disorders
US10029048B2 (en) 2014-05-13 2018-07-24 Allergan, Inc. High force injection devices
EP3954759A1 (en) 2014-05-16 2022-02-16 Janssen Biotech, Inc. Use of small molecules to enhance mafa expression in pancreatic endocrine cells
TW201603818A (zh) 2014-06-03 2016-02-01 組織科技股份有限公司 組成物及方法
US9949874B2 (en) 2014-06-06 2018-04-24 Janssen Biotech, Inc. Therapeutic agent delivery device with convergent lumen
US9925088B2 (en) 2014-06-06 2018-03-27 Janssen Biotech, Inc. Sub-retinal tangential needle catheter guide and introducer
US20160000550A1 (en) * 2014-07-05 2016-01-07 Deborah Nagle Methods for treating diseases of the colon
AU2015286462B2 (en) * 2014-07-11 2021-04-15 Lymphogenix Limited Cardiac cell culture material
US10064752B2 (en) 2014-09-11 2018-09-04 Orbit Biomedical Limited Motorized suprachoroidal injection of therapeutic agent
US10322028B2 (en) 2014-09-11 2019-06-18 Orbit Biomedical Limited Method and apparatus for sensing position between layers of an eye
US10219936B2 (en) 2014-09-11 2019-03-05 Orbit Biomedical Limited Therapeutic agent delivery device with advanceable cannula and needle
CN107075470A (zh) 2014-09-18 2017-08-18 北卡罗来纳大学 哺乳动物肺球体和肺球体细胞及其应用
US10258502B2 (en) 2014-09-18 2019-04-16 Orbit Biomedical Limited Therapeutic agent delivery device
US9504905B2 (en) 2014-09-19 2016-11-29 Bally Gaming, Inc. Card shuffling device and calibration method
KR101613478B1 (ko) * 2014-09-22 2016-04-19 (주)안트로젠 중간엽줄기세포-하이드로겔을 함유하는 조성물 및 이의 제조방법
US20160090569A1 (en) 2014-09-26 2016-03-31 Terumo Bct, Inc. Scheduled Feed
WO2016054591A1 (en) 2014-10-03 2016-04-07 Cedars-Sinai Medical Center Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of muscular dystrophy
CA2962954A1 (en) 2014-10-14 2016-04-21 Samuel Lynch Compositions for treating wounds
US20180280447A1 (en) * 2014-10-29 2018-10-04 Sungkwang Medical Foundation Placenta-derived cells excreting c3 or c1r complement and composition containing same
EP3212247B1 (en) * 2014-10-31 2021-04-07 The Administrators of the Tulane Educational Fund Surgical grafts for replacing the nipple and areola or damaged epidermis
RU2750695C2 (ru) 2014-11-07 2021-07-01 Экзостемтех Ко., Лтд. Композиция, включающая экзосому, полученную из стволовых клеток, для индукции адипогенной дифференцировки, регенерации жировой ткани, отбеливания кожи или коррекции морщин
US20160158293A1 (en) * 2014-12-05 2016-06-09 Janssen Biotech, Inc. Treatment of Ocular Conditions Using Progenitor Cells
WO2016086403A1 (en) * 2014-12-05 2016-06-09 Meridigen Biotech Co., Ltd. Method of distinguishing mesenchymal stem cells
JP2018500325A (ja) * 2014-12-16 2018-01-11 ヤンセン バイオテツク，インコーポレーテツド 前駆細胞を用いた網膜変性の治療法
US20170080033A1 (en) * 2014-12-16 2017-03-23 Janssen Biotech, Inc. Treatment of retinal degeneration using progenitor cells
CA2970935A1 (en) 2014-12-19 2016-06-23 Janssen Biotech, Inc. Suspension culturing of pluripotent stem cells
WO2016111899A1 (en) 2015-01-05 2016-07-14 Petrucci Gary M Methods and materials for treating lung disorders
WO2016126122A2 (ko) * 2015-02-04 2016-08-11 한양대학교 에리카산학협력단 연골세포로 분화되고 있는 줄기세포로부터 추출된 엑소좀을 포함하는 연골세포 분화 유도 또는 연골조직 재생용 조성물
US11077301B2 (en) 2015-02-21 2021-08-03 NeurostimOAB, Inc. Topical nerve stimulator and sensor for bladder control
US20160243288A1 (en) 2015-02-23 2016-08-25 Tissuetech, Inc. Apparatuses and methods for treating ophthalmic diseases and disorders
EP4008775A1 (en) 2015-03-04 2022-06-08 Mesoblast International Sàrl Cell culture method for mesenchymal stem cells
HK1249461A1 (zh) 2015-03-10 2018-11-02 Allergan Sales, Llc 多针注射器
JP6847044B2 (ja) 2015-03-11 2021-03-24 シーシーエス・ベンチャーズ・リミテッド 肥満及び２型糖尿病（ｔ２ｄ）の治療のための膵内分泌前駆細胞療法
US11027047B2 (en) 2015-03-31 2021-06-08 The University Of North Carolina At Chapel Hill Delivery vehicles for stem cells and uses thereof
US10286009B2 (en) * 2015-05-16 2019-05-14 Asterias Biotherapeutics, Inc. Pluripotent stem cell-derived oligodendrocyte progenitor cells for the treatment of spinal cord injury
EP3297645A4 (en) 2015-05-20 2018-12-12 Tissuetech, Inc. Compositions and methods for preventing the proliferation and epithelial-mesenchymal transition of epithelial cells
CA3177726A1 (en) 2015-05-21 2016-11-24 Musculoskeletal Transplant Foundation Modified demineralized cortical bone fibers
US10335435B2 (en) 2015-05-22 2019-07-02 Marco Merida Method for endoscopically delivering stem cells to the brain using an intranasal, injectable approach
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US10384207B2 (en) 2015-07-21 2019-08-20 Neuro Probe Incorporated Assay apparatus and methods
WO2017019832A1 (en) 2015-07-29 2017-02-02 Medivation Technologies, Inc. Methods and compositions using repair cells and cationic dyes
WO2017019822A1 (en) * 2015-07-29 2017-02-02 Medivation Technologies, Inc. Pellet composition containing repair cells
JP6998768B2 (ja) 2015-08-28 2022-02-10 ロート製薬株式会社 Ｒｏｒ１陽性の間葉系幹細胞及びその調製方法、ｒｏｒ１陽性の間葉系幹細胞を含む医薬組成物及びその調製方法、並びにｒｏｒ１陽性の間葉系幹細胞を用いる疾患の予防又は治療方法
USD797290S1 (en) 2015-10-19 2017-09-12 Spinal Surgical Strategies, Llc Bone graft delivery tool
WO2017087759A1 (en) 2015-11-18 2017-05-26 President And Fellows Of Harvard College Cartridge-based system for long term culture of cell clusters
US12551509B2 (en) 2015-11-18 2026-02-17 Lucina Patent Holdco, Llc Acellular regenerative products and methods of their manufacture
SG11201803781XA (en) * 2015-12-04 2018-06-28 Janssen Biotech Inc Treatment of retinal degeneration using progenitor cells
KR20170076484A (ko) * 2015-12-24 2017-07-04 삼성전자주식회사 프로토카데린의 과발현을 이용한 노화 세포를 분리하는 방법
US10814038B2 (en) 2016-01-06 2020-10-27 3-D Matrix, Ltd. Combination compositions
US11253551B2 (en) 2016-01-11 2022-02-22 Cedars-Sinai Medical Center Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of heart failure with preserved ejection fraction
CA3010792A1 (en) 2016-01-14 2017-07-20 DePuy Synthes Products, Inc. Composition and methods for cryopreservation of hutc
TW201733600A (zh) 2016-01-29 2017-10-01 帝聖工業公司 胎兒扶持組織物及使用方法
WO2017136557A1 (en) * 2016-02-05 2017-08-10 Petrucci Gary M Methods and materials for treating nerve injuries and neurological disorders
US11458224B2 (en) 2016-03-04 2022-10-04 University of Pittsburgh—of the Commonwealth System of Higher Education Ovarian-derived hydrogels for biomedical and biotechnology applications
US10478553B2 (en) 2016-03-09 2019-11-19 Orbit Biomedical Limited Apparatus for subretinal administration of therapeutic agent via a curved needle
GB201604304D0 (en) 2016-03-14 2016-04-27 Tigenix S A U Adipose tissue-derived stromal stem cells for use in treating refractory complex perianal fistulas in crohn's disease
EP3223181B1 (en) * 2016-03-24 2019-12-18 Sofradim Production System and method of generating a model and simulating an effect on a surgical repair site
JP2019509744A (ja) 2016-03-30 2019-04-11 アステリアス バイオセラピューティクス インコーポレイテッド オリゴデンドロサイト前駆細胞組成物
CN109310827B (zh) 2016-04-08 2021-09-07 阿勒根公司 抽吸和注射装置
CA3020905A1 (en) 2016-04-14 2017-10-19 Janssen Biotech, Inc. Differentiation of pluripotent stem cells to intestinal midgut endoderm cells
JP7108537B2 (ja) 2016-04-27 2022-07-28 ロート製薬株式会社 Ｃｄ２０１、ｃｄ４６、ｃｄ５６、ｃｄ１４７及びｃｄ１６５からなる群より選択される少なくとも１種の細胞表面マーカーを発現する間葉系幹細胞及びその調製方法、並びに上記間葉系幹細胞を含む医薬組成物及びその調製方法
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
WO2017210652A1 (en) 2016-06-03 2017-12-07 Cedars-Sinai Medical Center Cdc-derived exosomes for treatment of ventricular tachyarrythmias
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11000410B2 (en) 2016-06-17 2021-05-11 Gyroscope Therapeutics Limited Guide apparatus for tangential entry into suprachoroidal space
US10806629B2 (en) 2016-06-17 2020-10-20 Gyroscope Therapeutics Limited Injection device for subretinal delivery of therapeutic agent
US10646374B2 (en) 2016-06-17 2020-05-12 Orbit Biomedical Limited Apparatus and method to form entry bleb for subretinal delivery of therapeutic agent
MA45502A (fr) 2016-06-21 2019-04-24 Janssen Biotech Inc Génération de cellules bêta fonctionnelles dérivées de cellules souches pluripotentes humaines ayant une respiration mitochondriale glucose-dépendante et une réponse en sécrétion d'insuline en deux phases
US10639398B2 (en) 2016-07-05 2020-05-05 Lifecell Corporation Tissue matrices incorporating multiple tissue types
MA45623A (fr) * 2016-07-05 2019-05-15 Janssen Biotech Inc Traitement d'une maladie vasculaire rétinienne utilisant des cellules progénitrices
KR20190055088A (ko) * 2016-08-19 2019-05-22 리젠티스 코퍼레이션 점막 조직의 조직 재구성을 위한 세포외 매트릭스
EP3405204B1 (en) * 2016-08-26 2025-06-25 Restem Llc Composition and methods of using umbilical cord lining stem cells
EP3515459A4 (en) 2016-09-20 2020-08-05 Cedars-Sinai Medical Center CELLS DERIVED FROM CARDIOSPHERES AND THEIR EXTRACELLULAR VESICLES TO DELAY OR REVERSE AGING AND AGE-RELATED DISORDERS
MX2019005172A (es) 2016-11-02 2019-09-26 Axogen Corp Injertos de tejido amniotico y metodos para preparar y usar los mismos.
RU2644306C1 (ru) * 2016-11-22 2018-02-08 Общество с ограниченной ответственностью "ДЖИ-Групп" Способ восстановления дефектов покровных тканей
WO2018125851A1 (en) * 2016-12-26 2018-07-05 Michael Moeller Systems and methods to isolate and expand stem cells from urine
US11273072B2 (en) 2017-01-13 2022-03-15 Gyroscope Therapeutics Limited Suprachoroidal injection device
US10772986B2 (en) 2017-01-26 2020-09-15 Allosource Fascia fibrous compositions and methods for their use and manufacture
EP3593809A4 (en) 2017-03-08 2021-01-06 Rohto Pharmaceutical Co., Ltd. PHARMACEUTICAL COMPOSITION CONTAINING MMR1 POSITIVE MESENCHYMATIC STEM CELLS IN THE PROPHYLACTIC OR THERAPEUTIC TREATMENT OF A FIBROSIS-ASSOCIATED DISEASE, ITS PREPARATION PROCESS, AND PROCESS FOR THE PROPHYLACTIC TREATMENT OF FIBROSIS-ASSOCIATED DISEASE OR A FIBROSIS-ASSOCIATED THERAPEUTIC TREATMENT, AND PROCESS FOR THE PROPHYLACTIC TREATMENT OF FIBROSIS ASSOCIATED OR THERAPEUTIC TREATMENT MMR-POSITIVE MESENCHYMATIC STEM CELLS
US11076984B2 (en) 2017-03-13 2021-08-03 Gyroscope Therapeutics Limited Method of performing subretinal drainage and agent delivery
EP3595680A1 (en) 2017-03-17 2020-01-22 Rutgers, The State University of New Jersey Compositions and methods for wound healing
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
US12234441B2 (en) 2017-03-31 2025-02-25 Terumo Bct, Inc. Cell expansion
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
CA3059634A1 (en) 2017-04-13 2018-10-18 Senti Biosciences, Inc. Combinatorial cancer immunotherapy
CA3059910A1 (en) 2017-04-19 2018-10-25 Cedars-Sinai Medical Center Methods and compositions for treating skeletal muscular dystrophy
US10478531B2 (en) 2017-06-22 2019-11-19 Gary M. Petrucci Methods and materials for treating blood vessels
MX2019015316A (es) * 2017-06-29 2020-07-20 Xintela Ab Aseguramiento de la calidad de condrocitos.
JP7195033B2 (ja) 2017-07-18 2022-12-23 ザ リサーチ ファウンデイション フォー ザ ステイト ユニバーシティー オブ ニューヨーク 頭蓋内動脈瘤のためのバイオマーカー
US10251917B1 (en) 2017-09-19 2019-04-09 Gary M. Petrucci Methods and materials for treating tumors
US10821205B2 (en) 2017-10-18 2020-11-03 Lifecell Corporation Adipose tissue products and methods of production
US11123375B2 (en) 2017-10-18 2021-09-21 Lifecell Corporation Methods of treating tissue voids following removal of implantable infusion ports using adipose tissue products
WO2019079672A1 (en) 2017-10-19 2019-04-25 Lifecell Corporation ACELLULAR TISSUE MATRIX PRODUCTS FLUIDS AND METHODS OF PRODUCTION
US11246994B2 (en) 2017-10-19 2022-02-15 Lifecell Corporation Methods for introduction of flowable acellular tissue matrix products into a hand
US11629318B2 (en) 2017-10-20 2023-04-18 President And Fellows Of Harvard College Methods for producing mature adipocytes and methods of use thereof
US20210038650A1 (en) * 2017-10-23 2021-02-11 Cell Medicine, Inc. Mesenchymal stem cell therapy of leigh syndrome
WO2019087130A2 (en) * 2017-11-03 2019-05-09 Janssen Biotech, Inc. Method of inhibiting angiogenesis
KR102562469B1 (ko) 2017-11-07 2023-08-01 뉴로스팀 오에이비, 인크. 적응형 회로를 구비한 비침습성 신경 활성화기
WO2019126068A1 (en) 2017-12-20 2019-06-27 Cedars-Sinai Medical Center Engineered extracellular vesicles for enhanced tissue delivery
US11285177B2 (en) 2018-01-03 2022-03-29 Globus Medical, Inc. Allografts containing viable cells and methods thereof
CN111565732A (zh) 2018-01-12 2020-08-21 国立大学法人大阪大学 复层扁平上皮细胞的正常分化-成熟促进剂、上皮疾病治疗剂以及复层扁平上皮细胞的正常分化-成熟促进方法
US20190218521A1 (en) * 2018-01-18 2019-07-18 Lorenzo Bracco Culture medium of viruses for human vaccines have to consist of human cells from placenta and/or from umbilical cord of a fetus of the blood type 0 Rh- and of the mother of the blood type 0 Rh- (both, fetus and mother, must be of the blood type 0 Rh-)
WO2019152483A1 (en) * 2018-01-30 2019-08-08 University Of Georgia Research Foundation, Inc. Methods for vascular construction and products therefrom
WO2019152549A1 (en) 2018-02-05 2019-08-08 Cedars-Sinai Medical Center Methods for therapeutic use of exosomes and y-rnas
CN108384752A (zh) * 2018-02-13 2018-08-10 中国人民解放军第四五五医院 成软骨培养基及其在微分裂技术中膝关节软骨分化中的应用
US12453743B2 (en) 2018-05-30 2025-10-28 Direct Biologics, Llc Mesenchymal stem cell (MSC) growth factor and extracellular vesicle preparation in frozen or powdered form and methods of use
CN108961233A (zh) * 2018-06-28 2018-12-07 华侨大学 一种聚晶金刚石复合片表面缺陷分类识别方法
AU2019342741B2 (en) 2018-09-19 2025-12-11 Lineage Cell Therapeutics, Inc. Methods for differentiating pluripotent stem cells in dynamic suspension culture
US11419898B2 (en) 2018-10-17 2022-08-23 Senti Biosciences, Inc. Combinatorial cancer immunotherapy
JP2022512766A (ja) 2018-10-17 2022-02-07 センティ バイオサイエンシズ インコーポレイテッド 組み合わせがん免疫療法
JP7469317B2 (ja) 2019-01-23 2024-04-16 アステリアス バイオセラピューティクス インコーポレイテッド ヒト多能性幹細胞からの背側由来オリゴデンドロサイト前駆細胞
KR20210137026A (ko) 2019-02-07 2021-11-17 다이렉트 바이오로직스 엘엘씨 중간엽 줄기세포 엑소좀, 활액 중간엽 줄기세포 및 스캐폴드의 조합을 이용한 골관절염 치료 방법
US12391924B2 (en) 2019-02-08 2025-08-19 Regrow Biosciences Private Limited Method for mesenchymal stem cell isolation and osteoblast differentiation
US11759355B1 (en) 2019-02-26 2023-09-19 Gyroscope Therapeutics Limited Method of delivering leading blebs and agent to subretinal space
KR102169924B1 (ko) * 2019-03-26 2020-10-26 연세대학교 산학협력단 연골세포 분화 유도용 조성물 및 이의 용도
KR102167257B1 (ko) * 2019-05-28 2020-10-19 부산대학교 산학협력단 토마티딘 처리를 통한 줄기세포의 성숙화된 심근세포로의 분화유도 촉진 방법
AU2020283895A1 (en) 2019-05-30 2022-01-06 Lifecell Corporation Biologic breast implant
CN114126704B (zh) 2019-06-26 2026-02-03 神经科学技术有限责任公司 具有自适应电路的非侵入性神经激活器
EP3999083A4 (en) 2019-07-18 2023-07-26 Direct Biologics LLC Preparations comprising mesenchymal stem cells and cannabinoids and methods of their use
KR20220047879A (ko) * 2019-08-29 2022-04-19 아지노모토 가부시키가이샤 간엽계 줄기세포를 포함한 생체 유래 세포 시료로부터 간엽계 줄기세포를 제조하는 방법
US20220330530A1 (en) * 2019-09-13 2022-10-20 The University Of North Carolina At Chapel Hill Method of making human mouse xenografts
KR20220080145A (ko) * 2019-10-07 2022-06-14 유니버시티 오브 유타 리서치 파운데이션 연골원성 인간 중간엽 줄기 세포 (msc) 시트
WO2021069968A1 (en) 2019-10-11 2021-04-15 Gyroscope Therapeutics Limited Dose clip assembly for syringe
WO2021092199A1 (en) 2019-11-08 2021-05-14 Kansas State University Research Foundation Isolation, preservation, and expansion of canine umbilical cord mesenchymal stromal cells
TWI776276B (zh) * 2019-11-13 2022-09-01 中國醫藥大學 異種組織細胞組合物治療癌症之用途
EP4017580A4 (en) 2019-12-16 2023-09-06 Neurostim Technologies LLC NON-INVASIVE NERVE ACTIVATOR WITH ENHANCED CHARGE DELIVERY
US20230159932A1 (en) 2020-04-22 2023-05-25 Direct Biologics Llc Methods and compositions for treating inflammatory conditions associated with infectious disease
WO2021226373A2 (en) 2020-05-08 2021-11-11 Gallant Pet, Inc. Uterine-derived regenerative cell compositions and uses thereof
CN111718410B (zh) * 2020-06-05 2022-10-11 江南大学 一种制备卵黄免疫球蛋白的方法
JP6967308B1 (ja) * 2020-06-30 2021-11-17 国立大学法人高知大学 胎児付属物由来組織細胞培養上清を含む脳神経障害治療剤
CN113755432B (zh) * 2020-07-17 2022-06-10 上海我武干细胞科技有限公司 干细胞培养方法
WO2022046954A1 (en) * 2020-08-25 2022-03-03 Celularity Inc. Appl cells improved placental-derived adherent cells and methods of their use
CN114246985A (zh) * 2020-09-25 2022-03-29 华东理工大学 一种类骨膜组织的仿生构建方法
US12347100B2 (en) 2020-11-19 2025-07-01 Mazor Robotics Ltd. Systems and methods for generating virtual images
EP4259053A1 (en) 2020-12-22 2023-10-18 Gyroscope Therapeutics Limited Ocular cannula guide
EP4279919A4 (en) * 2021-01-12 2025-01-08 Cellatoz Therapeutics, Inc. Method for screening neuronal regeneration promoting cells having neuronal regeneration activity
CN116802501A (zh) * 2021-01-12 2023-09-22 雪拉托兹治疗株式会社 具有神经元再生活性的神经元再生促进细胞的筛选方法
EP4314244B1 (en) 2021-03-23 2025-07-23 Terumo BCT, Inc. Cell capture and expansion
WO2023111959A1 (en) * 2021-12-17 2023-06-22 3M Innovative Properties Company Wound dressings and methods of making the same
US12152699B2 (en) 2022-02-28 2024-11-26 Terumo Bct, Inc. Multiple-tube pinch valve assembly
US12435312B2 (en) 2022-02-28 2025-10-07 Brown University Quantifying cell-derived changes in collagen synthesis, alignment, and mechanics in a 3D connective tissue model
CN115068438B (zh) * 2022-04-28 2023-09-22 浙江大学医学院附属邵逸夫医院 破骨细胞前体同源靶向的细胞膜纳米囊泡制备方法及应用
ES2956802B2 (es) * 2022-05-20 2025-10-02 Bioiberica S A U Hidrogeles para su uso en ingenieria de tejidos de la piel
CN115044542B (zh) * 2022-06-30 2023-09-26 上海市东方医院（同济大学附属东方医院） Sj000291942在诱导间充质干细胞成骨分化方面的应用
JPWO2024024708A1 (es) * 2022-07-26 2024-02-01
USD1099116S1 (en) 2022-09-01 2025-10-21 Terumo Bct, Inc. Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device
JP2025527930A (ja) 2022-09-06 2025-08-22 ジェネンテック， インコーポレイテッド 二重湾曲針を介した治療剤の網膜下投与のための装置
EP4379046A1 (en) 2022-11-30 2024-06-05 Universidade Nova De Lisboa A 3d cellular model of early diabetic retinopathy
WO2024116114A1 (en) 2022-11-30 2024-06-06 Universidade Nova De Lisboa A 3d cellular model of early diabetic retinopathy
CN116218769B (zh) * 2023-01-03 2025-09-05 深圳市汉科生物工程有限公司 一种促进软骨细胞生长的方法
JP2026500975A (ja) 2023-01-13 2026-01-09 ジェネンテック， インコーポレイテッド シリンジのための投与ドック
WO2024172859A1 (en) * 2023-02-17 2024-08-22 Spine Biopharma, Inc. Peptide formulations
WO2025072590A1 (en) 2023-09-29 2025-04-03 Genentech, Inc. Ocular cannula guide, applicator, and marking instrument
WO2025080727A1 (en) * 2023-10-10 2025-04-17 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Autologous serum insert for treating an ocular condition
WO2025207699A1 (en) 2024-03-27 2025-10-02 Genentech, Inc. Torque ring for subretinal injection device
US12502407B2 (en) 2024-04-25 2025-12-23 Direct Biologics, Llc Treatment of fistula with bone marrow mesenchymal stem cell derived extracellular vesicles

Family Cites Families (326)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US229971A (en) 1880-07-13 Ttornets
US2324800A (en) 1941-08-14 1943-07-20 Pfizer Charles & Co Purification of riboflavin
US2654735A (en) * 1949-07-29 1953-10-06 Us Vitamin Corp Process for the production of derivatives of 9-polyhydroxyalkylisoalloxazines and products obtained
US2864848A (en) 1954-07-19 1958-12-16 Ca Nat Research Council Method of producing l-alpha-glycerylphosphorylcholine
US2912332A (en) * 1958-02-27 1959-11-10 Swift & Co Stabilized thiamine composition and method of enriching food products
US3665061A (en) 1969-07-16 1972-05-23 United States Banknote Corp Process for producing collagen sponges
JPS5651747B2 (es) 1973-05-31 1981-12-08
CH635748A5 (fr) 1977-08-16 1983-04-29 Cellorgan Laboratoires Sa Procede d'obtention de cellules placentaires de brebis.
US4216144A (en) * 1977-10-20 1980-08-05 Ashmead H H Soluble iron proteinates
JPS6040439B2 (ja) 1978-03-29 1985-09-11 大正製薬株式会社 ヒドロコルチゾン誘導体
US4352883A (en) 1979-03-28 1982-10-05 Damon Corporation Encapsulation of biological material
US4393240A (en) * 1981-07-06 1983-07-12 Stille John K Optically active phosphines
US4544516A (en) 1982-07-28 1985-10-01 Battelle Development Corporation Collagen orientation
US4465776A (en) 1982-09-27 1984-08-14 Research Corporation Monoclonal antibodies to vitamin B6 and immunoassay method
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
US4487865A (en) 1983-12-15 1984-12-11 Biomatrix, Inc. Polymeric articles modified with hyaluronate
US5902741A (en) 1986-04-18 1999-05-11 Advanced Tissue Sciences, Inc. Three-dimensional cartilage cultures
US4963489A (en) 1987-04-14 1990-10-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US5863531A (en) * 1986-04-18 1999-01-26 Advanced Tissue Sciences, Inc. In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework
US5266480A (en) 1986-04-18 1993-11-30 Advanced Tissue Sciences, Inc. Three-dimensional skin culture system
US4925667A (en) 1986-05-27 1990-05-15 Qmax Technology Group, Inc. Substrate with particulate cosmetic
JPS642643A (en) 1987-06-26 1989-01-06 Bio Material Yunibaasu:Kk Artificial skin
NZ226750A (en) 1987-10-29 1990-09-26 Amrad Corp Ltd Immortalisation of neural precursor cells by introducing a retrovirus vector containing a myc-oncogene
US5192553A (en) * 1987-11-12 1993-03-09 Biocyte Corporation Isolation and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and methods of therapeutic use
US5004681B1 (en) * 1987-11-12 2000-04-11 Biocyte Corp Preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood
US5162405A (en) * 1987-12-24 1992-11-10 Elf Atochem North America, Inc. Single-functional and mixtures of multi-functional oligomeric performance additive compositions and their uses
GB8803697D0 (en) 1988-02-17 1988-03-16 Deltanine Research Ltd Clinical developments using amniotic membrane cells
US4963439A (en) 1988-04-19 1990-10-16 Ube Industries, Ltd. Continuous fiber-reinforced Al-Co alloy matrix composite
US20030032178A1 (en) * 1988-08-04 2003-02-13 Williams Robert Lindsay In vitro propagation of embryonic stem cells
US5618670A (en) * 1988-08-26 1997-04-08 The United States Of America As Represented By The Department Of Health & Human Services Detection method for c-raf-1 genes
US4925677A (en) 1988-08-31 1990-05-15 Theratech, Inc. Biodegradable hydrogel matrices for the controlled release of pharmacologically active agents
US5284766A (en) * 1989-02-10 1994-02-08 Kao Corporation Bed material for cell culture
JPH06104061B2 (ja) 1989-02-10 1994-12-21 花王株式会社 細胞培養支持体材料
DE69009237T2 (de) * 1989-02-27 1994-12-01 Takasago Perfumery Co Ltd Verfahren zur Herstellung von optisch aktiven Estern der 6-t-Butoxy-3,5-dihydroxyhexansäure.
FR2646438B1 (fr) 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
US5437994A (en) * 1989-06-15 1995-08-01 Regents Of The University Of Michigan Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
US5574205A (en) 1989-07-25 1996-11-12 Cell Genesys Homologous recombination for universal donor cells and chimeric mammalian hosts
US5840580A (en) 1990-05-01 1998-11-24 Becton Dickinson And Company Phenotypic characterization of the hematopoietic stem cell
US5145770A (en) * 1990-06-04 1992-09-08 Biosurface Technology, Inc. Cryopreservation of cultured epithelial sheets
WO1992003917A1 (en) 1990-08-29 1992-03-19 Genpharm International Homologous recombination in mammalian cells
US5336616A (en) * 1990-09-12 1994-08-09 Lifecell Corporation Method for processing and preserving collagen-based tissues for transplantation
US5342761A (en) 1990-10-01 1994-08-30 Research Development Foundation Oncofetal gene, gene product and uses therefor
US5506134A (en) 1990-10-22 1996-04-09 Corvas International, Inc. Hypridoma and monoclonal antibody which inhibits blood coagulation tissue factor/factor VIIa complex
US5811094A (en) 1990-11-16 1998-09-22 Osiris Therapeutics, Inc. Connective tissue regeneration using human mesenchymal stem cell preparations
US5486359A (en) * 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
US5140100A (en) * 1990-12-28 1992-08-18 Cedars-Sinai Medical Center Protein that inhibits production of human choriogonadotropin
US5286632A (en) * 1991-01-09 1994-02-15 Jones Douglas H Method for in vivo recombination and mutagenesis
NL9100038A (nl) 1991-01-11 1992-08-03 Stamicarbon Enzym-gekatalyseerde bereiding van optisch aktieve carbonzuren.
WO1992019249A1 (en) 1991-05-02 1992-11-12 Yeda Research And Development Co. Ltd. Compositions for the prevention and/or treatment of pathological processes
US6399369B1 (en) * 1991-07-08 2002-06-04 Neurospheres Holdings Ltd. Multipotent neural stem cell cDNA libraries
EP0552380A4 (en) 1991-08-08 1995-01-25 Kao Corp Cell culture support, production thereof, and production of cell cluster using same
EP0529751A1 (en) 1991-08-09 1993-03-03 W.R. Grace & Co.-Conn. Cell culture substrate, test material for cell culture and preparations thereof
WO1993004169A1 (en) 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
WO1993006726A1 (en) * 1991-09-30 1993-04-15 Mackenzie Walser Methods for treatment of free-radical-mediated tissue injury
US5308763A (en) * 1991-10-01 1994-05-03 The Johns Hopkins University Method of making primary culture of olfactory neurons
US5914265A (en) * 1992-04-30 1999-06-22 Baylor College Of Medicine Keratin K1 expression vectors and methods of use
AU4543193A (en) 1992-06-22 1994-01-24 Henry E. Young Scar inhibitory factor and use thereof
US5320962A (en) 1992-07-22 1994-06-14 Duke University DNA encoding the human A1 adenosine receptor
US5589376A (en) 1992-07-27 1996-12-31 California Institute Of Technology Mammalian neural crest stem cells
US5356807A (en) * 1992-09-08 1994-10-18 Cornell Research Foundation Cultured cell line of adult diploid cells from human brain and meningeal tissue
US20040224409A1 (en) 1992-09-25 2004-11-11 Laurent Pradier Recombinant adenoviruses coding for brain-derived neurotrophic factor (BDNF)
WO1994010331A1 (en) 1992-10-29 1994-05-11 The Australian National University Angiogenesis inhibitory antibodies
US5670483A (en) 1992-12-28 1997-09-23 Massachusetts Insititute Of Technology Stable macroscopic membranes formed by self-assembly of amphiphilic peptides and uses therefor
US5955343A (en) 1992-12-28 1999-09-21 Massachusetts Institute Of Technology Stable macroscopic membranes formed by self-assembly of amphiphilic peptides and uses therefor
US5707643A (en) 1993-02-26 1998-01-13 Santen Pharmaceutical Co., Ltd. Biodegradable scleral plug
WO1994022451A1 (en) * 1993-04-07 1994-10-13 Oklahoma Medical Research Foundation Selective regulation of b lymphocyte precursors by hormones
WO1994025584A1 (en) 1993-04-28 1994-11-10 Johns Hopkins University School Of Medicine Chronic endothelial cell culture under flow
IL110589A0 (en) 1993-08-10 1994-11-11 Bioph Biotech Entw Pharm Gmbh Growth/differentiation factor of the TGF- beta family
JP3680114B2 (ja) 1993-09-17 2005-08-10 敏一 中村 脳神経障害治療剤
US6686198B1 (en) 1993-10-14 2004-02-03 President And Fellows Of Harvard College Method of inducing and maintaining neuronal cells
US6432711B1 (en) 1993-11-03 2002-08-13 Diacrin, Inc. Embryonic stem cells capable of differentiating into desired cell lines
US5456835A (en) * 1993-11-08 1995-10-10 Hemasure, Inc. Device and process for removing free hemoglobin from blood
DE4406073A1 (de) * 1994-02-24 1995-08-31 Univ Ludwigs Albert Verfahren zur Herstellung von humanen, klonogenen Fibroblasten, Verfahren zur Gentransfizierung von Fibroblasten und so erhaltene Fibroblasten
US5698518A (en) 1994-03-30 1997-12-16 Oklahoma Medical Research Foundation Method for regulating inflammation and tumor growth with calmodulin, calmodulin analogues or calmodulin antagonists
US5466233A (en) 1994-04-25 1995-11-14 Escalon Ophthalmics, Inc. Tack for intraocular drug delivery and method for inserting and removing same
US6001647A (en) 1994-04-28 1999-12-14 Ixion Biotechnology, Inc. In vitro growth of functional islets of Langerhans and in vivo uses thereof
US6703017B1 (en) 1994-04-28 2004-03-09 Ixion Biotechnology, Inc. Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures
US5834308A (en) 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans
CA2192103C (en) 1994-06-06 2002-02-05 Arnold I. Caplan Biomatrix for tissue regeneration
IL114397A0 (en) 1994-07-01 1995-10-31 Bioph Biotech Entw Pharm Gmbh Growth/differentiation factor of the TGF-beta-family
US5935849A (en) * 1994-07-20 1999-08-10 Cytotherapeutics, Inc. Methods and compositions of growth control for cells encapsulated within bioartificial organs
US6309853B1 (en) 1994-08-17 2001-10-30 The Rockfeller University Modulators of body weight, corresponding nucleic acids and proteins, and diagnostic and therapeutic uses thereof
US5660982A (en) * 1994-10-04 1997-08-26 Tryggvason; Karl Laminin chains: diagnostic uses
US5789147A (en) 1994-12-05 1998-08-04 New York Blood Center, Inc. Method for concentrating white cells from whole blood by adding a red cell sedimentation reagent to whole anticoagulated blood
US5725493A (en) * 1994-12-12 1998-03-10 Avery; Robert Logan Intravitreal medicine delivery
US5684032A (en) 1994-12-13 1997-11-04 Smithkline Beecham Corporation Compounds
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5736396A (en) 1995-01-24 1998-04-07 Case Western Reserve University Lineage-directed induction of human mesenchymal stem cell differentiation
US5906934A (en) 1995-03-14 1999-05-25 Morphogen Pharmaceuticals, Inc. Mesenchymal stem cells for cartilage repair
US5718922A (en) * 1995-05-31 1998-02-17 Schepens Eye Research Institute, Inc. Intravitreal microsphere drug delivery and method of preparation
US5869079A (en) * 1995-06-02 1999-02-09 Oculex Pharmaceuticals, Inc. Formulation for controlled release of drugs by combining hydrophilic and hydrophobic agents
US5693332C1 (en) 1995-08-11 2001-01-09 Univ California Human keratinocytes supported on a hydrophilic membrane and methods of using same to effect wound closure
US5641750A (en) * 1995-11-29 1997-06-24 Amgen Inc. Methods for treating photoreceptors using glial cell line-derived neurotrophic factor (GDNF) protein product
US6200606B1 (en) * 1996-01-16 2001-03-13 Depuy Orthopaedics, Inc. Isolation of precursor cells from hematopoietic and nonhematopoietic tissues and their use in vivo bone and cartilage regeneration
US5842477A (en) 1996-02-21 1998-12-01 Advanced Tissue Sciences, Inc. Method for repairing cartilage
CA2248549A1 (en) 1996-03-15 1997-09-18 Munin Corporation Extracellular matrix signalling molecules
US6223188B1 (en) * 1996-04-10 2001-04-24 Sun Microsystems, Inc. Presentation of link information as an aid to hypermedia navigation
AU2808397A (en) 1996-04-26 1997-11-19 Case Western Reserve University Skin regeneration using mesenchymal stem cells
US6358737B1 (en) 1996-07-31 2002-03-19 Board Of Regents, The University Of Texas System Osteocyte cell lines
US6787355B1 (en) 1996-08-26 2004-09-07 Mcgill University Multipotent neural stem cells from peripheral tissues and uses thereof
US5919702A (en) 1996-10-23 1999-07-06 Advanced Tissue Science, Inc. Production of cartilage tissue using cells isolated from Wharton's jelly
WO1998031316A1 (en) * 1997-01-17 1998-07-23 Celadon Science, Llc Methods for promoting healing of corneal resurfacing wounds
AU6144698A (en) 1997-02-05 1998-08-25 Case Western Reserve University Stimulatory effects of bfgf and bmp-2 on osteogenic differentiation of mesenchymal stem cells
WO1998051317A1 (en) 1997-05-13 1998-11-19 Osiris Therapeutics, Inc. Osteoarthritis cartilage regeneration using human mesenchymal stem cells
CA2290381A1 (en) 1997-05-21 1998-11-26 Sloan-Kettering Institute For Cancer Research Method for increasing the concentration of ascorbic acid in brain tissues of a subject
AU738334B2 (en) 1997-05-30 2001-09-13 Osteobiologics, Inc. Fiber-reinforced, porous, biodegradable implant device
ATE307195T1 (de) 1997-07-14 2005-11-15 Osiris Therapeutics Inc Herzmuskelregenerierung unter verwendung mesenchymaler stammzellen
US5902598A (en) * 1997-08-28 1999-05-11 Control Delivery Systems, Inc. Sustained release drug delivery devices
ES2351082T3 (es) * 1997-12-02 2011-01-31 Artecel Sciences, Inc. Diferenciación de células estromales adiposas en osteoblastos y sus usos.
US6059968A (en) 1998-01-20 2000-05-09 Baxter International Inc. Systems for processing and storing placenta/umbilical cord blood
US6291240B1 (en) * 1998-01-29 2001-09-18 Advanced Tissue Sciences, Inc. Cells or tissues with increased protein factors and methods of making and using same
PT1062321E (pt) 1998-03-13 2005-05-31 Osiris Therapeutics Inc Utilizacoes para celulas estaminais mesenquimais humanas nao autologas
EP1076563B1 (en) 1998-03-16 2005-05-11 Cytovia, Inc. Dipeptide caspase inhibitors and the use thereof
US6179872B1 (en) 1998-03-17 2001-01-30 Tissue Engineering Biopolymer matt for use in tissue repair and reconstruction
US6171610B1 (en) 1998-04-24 2001-01-09 University Of Massachusetts Guided development and support of hydrogel-cell compositions
JP2002513545A (ja) 1998-05-07 2002-05-14 ザ ユニヴァーシティー オブ サウス フロリダ 脳および脊髄修復のためのニューロン源としての骨髄細胞
ES2292245T3 (es) * 1998-05-29 2008-03-01 Osiris Therapeutics, Inc. Celulas madre mesenquimaticas cd45+ y/o fibroblasto+humanas.
US6323188B1 (en) 1998-07-01 2001-11-27 Donald L. Weissman Treatment and prevention of cardiovascular diseases, heart attack, and stroke, primary and subsequent, with help of aspirin and certain vitamins
DE19833340A1 (de) 1998-07-24 2000-02-10 Karlsruhe Forschzent Wurmförmiger Arbeitsmechanismus
US20040037818A1 (en) 1998-07-30 2004-02-26 Brand Stephen J. Treatment for diabetes
ATE445006T1 (de) 1998-08-10 2009-10-15 Us Gov Health & Human Serv Differenzierung von nicht-insulin in insulin- produzierende zellen durch glp-1 und exendin-4 und dessen verwendung
US5958767A (en) * 1998-08-14 1999-09-28 The Children's Medical Center Corp. Engraftable human neural stem cells
US6284245B1 (en) 1998-08-25 2001-09-04 Diacrin, Inc. Neural retinal cells and retinal pigment epithelium cells and their use in treatment of retinal disorders
US6444205B2 (en) 1998-09-30 2002-09-03 Diacrin, Inc. Transplantation of neural cells for the treatment of chronic pain or spasticity
US6610540B1 (en) 1998-11-18 2003-08-26 California Institute Of Technology Low oxygen culturing of central nervous system progenitor cells
US6241369B1 (en) * 1998-11-20 2001-06-05 Cooper Technologies Company Quick mount fixture
US6144054A (en) * 1998-12-04 2000-11-07 International Business Machines Corporation DRAM cell having an annular signal transfer region
DE19856428C1 (de) * 1998-12-08 2000-05-04 Heraeus Noblelight Gmbh Entladungslampe
AU1675600A (en) 1998-12-24 2000-07-31 Biosafe S.A. Blood separation system particularly for concentrating hematopoietic stem cells
JP4932069B2 (ja) 1999-01-25 2012-05-16 株式会社セルシード アクリルアミド誘導体および該誘導体を含む重合体
CA2359821A1 (en) 1999-02-04 2000-08-10 Mcgill University Platform for the differentiation of cells
AU780794B2 (en) 1999-02-10 2005-04-14 Es Cell International Pte Ltd Pancreatic progenitor cells, methods and uses related thereto
US6592623B1 (en) 1999-08-31 2003-07-15 Virginia Commonwealth University Intellectual Property Foundation Engineered muscle
RU2306335C2 (ru) 1999-03-10 2007-09-20 Юниверсити Оф Питтсбург Оф Дзе Коммонвелт Систем Оф Хайер Эдьюкейшн Стволовые клетки и решетки, полученные из жировой ткани
US20030007954A1 (en) 1999-04-12 2003-01-09 Gail K. Naughton Methods for using a three-dimensional stromal tissue to promote angiogenesis
JP2002542349A (ja) 1999-04-16 2002-12-10 ダブリューエム・マーシュ・ライス・ユニバーシティー ポリ（プロピレンフマラート）−ジアクリレートマクロマーで交差結合された生体分解性のポリ（プロピレンフマラート）ネットワーク
US7371400B2 (en) 2001-01-02 2008-05-13 The General Hospital Corporation Multilayer device for tissue engineering
US6261600B1 (en) 1999-04-30 2001-07-17 Drugtech Corporation Folic acid supplement
US6287340B1 (en) 1999-05-14 2001-09-11 Trustees Of Tufts College Bioengineered anterior cruciate ligament
US6372494B1 (en) 1999-05-14 2002-04-16 Advanced Tissue Sciences, Inc. Methods of making conditioned cell culture medium compositions
AU4860900A (en) 1999-06-02 2000-12-18 Lifebank Services, L.L.C. Methods of isolation, cryopreservation, and therapeutic use of human amniotic epithelial cells
US6329904B1 (en) * 1999-06-11 2001-12-11 Safety Through Cellular, Inc. Apparatus and method for providing weather and other alerts
WO2001000788A2 (en) 1999-06-25 2001-01-04 Northwestern University Compositions, kits, and methods for modulating survival and differentiation of multi-potential hematopoietic progenitor cells
US6333029B1 (en) * 1999-06-30 2001-12-25 Ethicon, Inc. Porous tissue scaffoldings for the repair of regeneration of tissue
US6355699B1 (en) 1999-06-30 2002-03-12 Ethicon, Inc. Process for manufacturing biomedical foams
ATE514772T1 (de) 1999-08-05 2011-07-15 Abt Holding Co Multipotente erwachsene stammzellen und verfahren zu deren isolierung
US6429013B1 (en) 1999-08-19 2002-08-06 Artecel Science, Inc. Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair
US6555374B1 (en) 1999-08-19 2003-04-29 Artecel Sciences, Inc. Multiple mesodermal lineage differentiation potentials for adipose tissue-derived stromal cells and uses thereof
EP1216049A2 (en) 1999-09-14 2002-06-26 Children's Medical Center Corporation Methods for treating muscular dystrophy with bone marrow cells
US6331313B1 (en) 1999-10-22 2001-12-18 Oculex Pharmaceticals, Inc. Controlled-release biocompatible ocular drug delivery implant devices and methods
US20030129745A1 (en) 1999-10-28 2003-07-10 Robl James M. Gynogenetic or androgenetic production of pluripotent cells and cell lines, and use thereof to produce differentiated cells and tissues
EP1099754A1 (en) 1999-11-10 2001-05-16 Universiteit Leiden Mesenchymal stem cells and/or progenitor cells, their isolation and use
EP1257282A4 (en) 1999-12-06 2003-05-02 Gen Hospital Corp PANCREATIC STEM CELLS AND THEIR USE IN TRANSPLANTATION
US20030082155A1 (en) * 1999-12-06 2003-05-01 Habener Joel F. Stem cells of the islets of langerhans and their use in treating diabetes mellitus
US20020164307A1 (en) 1999-12-06 2002-11-07 Habener Joel F. Stem cells of the islets of langerhans and their use in treating diabetes mellitus
WO2001053503A1 (en) 2000-01-18 2001-07-26 Cornell Research Foundation, Inc. Neural progenitor cells from hippocampal tissue and a method for isolating and purifying them
US7544509B2 (en) 2000-01-24 2009-06-09 Mcgill University Method for preparing stem cell preparations
US6610535B1 (en) 2000-02-10 2003-08-26 Es Cell International Pte Ltd. Progenitor cells and methods and uses related thereto
JP2003521935A (ja) 2000-02-11 2003-07-22 フイラデルフイア・ヘルス・アンド・エデユケーシヨン・コーポレーシヨン 骨髄細胞のニューロン細胞への分化およびそのための使用
AU2001234998B2 (en) 2000-02-11 2006-06-08 Childrens Hospital Of Orange County A California Corporation Isolation and transplantation of retinal stem cells
US7160724B2 (en) * 2000-03-09 2007-01-09 University Of South Florida Human cord blood as a source of neural tissue for repair of the brain and spinal cord
AU2001245550A1 (en) * 2000-03-09 2001-09-17 Lee Walters Applications of immune system tolerance to treatment of various diseases
EP2298858A1 (en) 2000-03-16 2011-03-23 Cellseed Inc. Bed material for cell culture, method for co-culture of cell and co-cultured cell sheet obtainable therefrom
US6436704B1 (en) 2000-04-10 2002-08-20 Raven Biotechnologies, Inc. Human pancreatic epithelial progenitor cells and methods of isolation and use thereof
US6673606B1 (en) * 2000-04-12 2004-01-06 The Children's Hospital Of Philadelphia Therapeutic uses for mesenchymal stromal cells
US6375972B1 (en) 2000-04-26 2002-04-23 Control Delivery Systems, Inc. Sustained release drug delivery devices, methods of use, and methods of manufacturing thereof
AU6459801A (en) 2000-05-12 2001-11-26 Mark T Keating Compositions and methods for cell dedifferentiation and tissue regeneration
US8273570B2 (en) 2000-05-16 2012-09-25 Riken Process of inducing differentiation of embryonic cell to cell expressing neural surface marker using OP9 or PA6 cells
US7049072B2 (en) 2000-06-05 2006-05-23 University Of South Florida Gene expression analysis of pluri-differentiated mesenchymal progenitor cells and methods for diagnosing a leukemic disease state
US6759039B2 (en) 2000-06-30 2004-07-06 Amcyte, Inc. Culturing pancreatic stem cells having a specified, intermediate stage of development
EP2275532B1 (en) 2000-07-21 2012-06-06 Cellseed Inc. Myocardium-like cell sheets and processes for producing them
JP4716479B2 (ja) * 2000-07-21 2011-07-06 株式会社セルシード 表皮培養細胞シート、重層化培養皮膚シート、及びそれらの製造法
US6984522B2 (en) 2000-08-03 2006-01-10 Regents Of The University Of Michigan Isolation and use of solid tumor stem cells
DE10038814A1 (de) * 2000-08-09 2002-02-21 Abb Research Ltd Hochspannungs-Gleichstromwandler
CA2424062A1 (en) 2000-09-29 2002-04-04 Derek Van Der Kooy Primitive neural stem cells and method for differentiation of stem cells to neural cells
US6639470B1 (en) 2000-10-06 2003-10-28 Skyworks Solutions, Inc. Constant current biasing circuit for linear power amplifiers
US7560280B2 (en) 2000-11-03 2009-07-14 Kourion Therapeutics Gmbh Human cord blood derived unrestricted somatic stem cells (USSC)
US20020098584A1 (en) 2000-11-06 2002-07-25 Palmer Theo D. Postmortem stem cells
JP2004532648A (ja) 2000-11-30 2004-10-28 ステムロン インコーポレイテッド 単離されたホモ接合性幹細胞、これ由来の分化細胞、ならびにこれを作製及び使用するための材料及び方法
DK1349918T3 (da) 2000-12-06 2014-11-10 Anthrogenesis Corp Fremgangsmåde til indsamling af stamceller fra moderkagen
US7311905B2 (en) 2002-02-13 2007-12-25 Anthrogenesis Corporation Embryonic-like stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells
US7311904B2 (en) 2001-02-14 2007-12-25 Anthrogenesis Corporation Tissue matrices comprising placental stem cells, and methods of making the same
US6599323B2 (en) 2000-12-21 2003-07-29 Ethicon, Inc. Reinforced tissue implants and methods of manufacture and use
CA2365376C (en) 2000-12-21 2006-03-28 Ethicon, Inc. Use of reinforced foam implants with enhanced integrity for soft tissue repair and regeneration
WO2002059278A2 (en) 2001-01-24 2002-08-01 The Government Of The United States Of America, As Represented By The Secretary Of Department Of Health & Human Services Differentiation of stem cells to pancreatic endocrine cells
WO2002061053A1 (en) 2001-01-31 2002-08-08 The General Hospital Corporation Renal stem cells and uses thereof
US7449180B2 (en) 2001-02-06 2008-11-11 John Kisiday Macroscopic scaffold containing amphiphilic peptides encapsulating cells
DE60230593D1 (de) 2001-02-06 2009-02-12 Massachusetts Inst Technology Peptidgerüstverkapselung von gewebszellen und verwendungen davon
IL157350A0 (en) * 2001-02-14 2004-02-19 Anthrogenesis Corp Post-partum mammalian placenta, its use and placental stem cells therefrom
EP1367899A4 (en) 2001-02-14 2004-07-28 Leo T Furcht TOTIPOTENT ADULT STEM CELLS, SOURCES OF SUCH CELLS, METHODS FOR OBTAINING AND MAINTAINING SAME, METHODS FOR DIFFERENTIATING THESE CELLS, METHODS OF USING SAME, AND CELLS DERIVED FROM THE ABOVE-MENTIONED CELLS
CA2441986A1 (en) * 2001-03-27 2002-10-03 Massachusetts Institute Of Technology Methods and products related to fgf dimerization
US7838292B1 (en) 2001-03-29 2010-11-23 University Of Louisville Research Foundation, Inc. Methods for obtaining adult human olfactory progenitor cells
US7202080B2 (en) 2001-03-29 2007-04-10 Ixion Biotechnology, Inc. Method for transdifferentiation of non-pancreatic stem cells to the pancreatic differentiation pathway
WO2002086107A2 (en) 2001-04-19 2002-10-31 DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung A method for differentiating stem cells into insulin-producing cells
KR100762261B1 (ko) 2001-04-27 2007-10-04 (주)바이오니아 전장 상보 디옥시리보핵산 제조 방법과 이에 사용되는앵커와 프라이머
US20030211605A1 (en) 2001-05-01 2003-11-13 Lee Sang-Hun Derivation of midbrain dopaminergic neurons from embryonic stem cells
US20030022369A1 (en) * 2001-05-18 2003-01-30 Helen Fillmore Differentiation of specialized dermal and epidermal cells into neuronal cells
WO2002096203A1 (en) 2001-05-25 2002-12-05 Cythera, Inc. Stem cell differentiation
AU2002345603B2 (en) 2001-06-07 2006-10-12 Skinmedica, Inc. Conditioned cell culture media and uses thereof
RU2183966C1 (ru) 2001-07-06 2002-06-27 Общество с ограниченной ответственностью "Сибларекс" Состав биофлавоноидного комплекса сибларекс для биологически активных добавок, медицинских и химико-фармацевтических изделий и способ получения биофлавоноидного комплекса сибларекс для биологически активных добавок, медицинских и химико-фармацевтических изделий
US6402263B1 (en) 2001-07-24 2002-06-11 Robert Bosch Corporation Dual actuation master cylinder
EP1423504B1 (en) 2001-08-08 2009-09-23 Levesque Biosciences, Inc. Compositions and methods for isolation, propagation, and differentiation of non-embryonic human stem cells and uses thereof
US20030211603A1 (en) 2001-08-14 2003-11-13 Earp David J. Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells
AU2002313817A1 (en) * 2001-08-27 2003-03-10 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
US20030104997A1 (en) * 2001-09-05 2003-06-05 Black Ira B. Multi-lineage directed induction of bone marrow stromal cell differentiation
CN1195055C (zh) 2001-09-06 2005-03-30 周胜利 从胎盘组织中提取造血干细胞用于建立造血干细胞库的新方法
US20050064587A1 (en) 2001-09-07 2005-03-24 Lawrence Rosenberg Pancreatic small cells and uses thereof
US9969980B2 (en) 2001-09-21 2018-05-15 Garnet Biotherapeutics Cell populations which co-express CD49c and CD90
US7072332B2 (en) * 2001-09-27 2006-07-04 Samsung Electronics Co., Ltd. Soft switch using distributed firewalls for load sharing voice-over-IP traffic in an IP network
EP1298201A1 (en) 2001-09-27 2003-04-02 Cardion AG Process for the production of cells exhibiting an islet-beta-cell-like state
GB2396623B (en) 2001-09-28 2006-04-05 Es Cell Int Pte Ltd Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers
WO2003033697A1 (en) 2001-10-18 2003-04-24 Ixion Biotechnology, Inc. Conversion of liver stem and progenitor cells to pancreatic functional cells
US7129034B2 (en) 2001-10-25 2006-10-31 Cedars-Sinai Medical Center Differentiation of whole bone marrow
US20030124721A1 (en) 2001-11-09 2003-07-03 Bentley Cheatham Endocrine pancreas differentiation of adipose tissue-derived stromal cells and uses thereof
WO2003054146A2 (en) 2001-11-14 2003-07-03 Northwestern University Self-assembly and mineralization of peptide-amphiphile nanofibers
WO2003042405A2 (en) 2001-11-15 2003-05-22 Children's Medical Center Corporation Methods of isolation, expansion and differentiation of fetal stem cells from chorionic villus, amniotic fluid, and placenta and therapeutic uses thereof
JP3728750B2 (ja) * 2001-11-22 2005-12-21 ニプロ株式会社 培養皮膚及びその製造方法
CN1596130A (zh) 2001-11-28 2005-03-16 安琪士多摩奇株式会社 治疗神经退行性疾病的基因治疗药物
US6712850B2 (en) 2001-11-30 2004-03-30 Ethicon, Inc. Porous tissue scaffolds for the repair and regeneration of dermal tissue
WO2003048336A2 (en) 2001-12-04 2003-06-12 Organogenesis Inc. Cultured cells from pancreatic islets
WO2003054171A1 (en) 2001-12-06 2003-07-03 The Regents Of The University Of California Method for differentiating islet precursor cells into beta cells
TW200301132A (en) * 2001-12-06 2003-07-01 Sankyo Co Pharmaceutical compositions containing cells derived from human caul
US7033831B2 (en) * 2001-12-07 2006-04-25 Geron Corporation Islet cells from human embryonic stem cells
AU2002364558A1 (en) * 2001-12-11 2003-06-23 Cytograft Tissue Engineering, Inc. Tissue engineered cellular sheets, methods of making and use thereof
JP3934539B2 (ja) 2001-12-12 2007-06-20 独立行政法人科学技術振興機構 胎盤等由来の成体又は生後組織の前駆細胞
US20030113910A1 (en) * 2001-12-18 2003-06-19 Mike Levanduski Pluripotent stem cells derived without the use of embryos or fetal tissue
IL162648A0 (en) * 2001-12-21 2005-11-20 Mount Sinai Hospital Corp Cellular compositions and methods of making and using them
US7101546B2 (en) 2001-12-21 2006-09-05 Amcyte, Inc. In situ maturation of cultured pancreatic stem cells having a specified, intermediate stage of development
JP2005512593A (ja) 2001-12-28 2005-05-12 セルアーティス アーベー 多能性のヒト胚盤胞由来幹細胞株の樹立方法
AU2003202988A1 (en) * 2002-01-14 2003-07-30 The Board Of Trustees Of The University Of Illinois Use of modified pyrimidine compounds to promote stem cell migration and proliferation
US20030158089A1 (en) 2002-01-24 2003-08-21 Xenoport, Inc. Administrative agents via the SMVT transporter
US20030162290A1 (en) 2002-01-25 2003-08-28 Kazutomo Inoue Method for inducing differentiation of embryonic stem cells into functioning cells
AU2003217357A1 (en) 2002-02-07 2003-09-02 The Research Foundation Of The State University Of New York Generation of new insulin cells from progenitor cells present in adult pancreatic islets
AU2003216286B2 (en) * 2002-02-13 2008-08-28 Celularity Inc. Embryonic-like stem cells derived from post-partum mammalian placenta and uses and methods of treatment using said cells
US7576065B2 (en) 2002-02-15 2009-08-18 Cornell Research Foundation, Inc. Enhancing neurotrophin-induced neurogenesis by endogenous neural progenitor cells by concurrent overexpression of brain derived neurotrophic factor and an inhibitor of a pro-gliogenic bone morphogenetic protein
AU2003215280A1 (en) 2002-02-15 2003-09-09 Northwestern University Self-assembly of peptide-amphiphile nanofibers under physiological conditions
WO2003070922A1 (en) 2002-02-19 2003-08-28 Medipost Co., Ltd. Isolation and culture-expansion methods of mesenchymal stem/progenitor cells from umbilical cord blood, and differentiation method of umbilical cord blood-derived mesenchymal stem/progenitor cells into various mesenchymal tissues
WO2003072728A2 (en) 2002-02-22 2003-09-04 University Of Florida Cellular trans-differentiation
US20030161818A1 (en) 2002-02-25 2003-08-28 Kansas State University Research Foundation Cultures, products and methods using stem cells
US7736892B2 (en) 2002-02-25 2010-06-15 Kansas State University Research Foundation Cultures, products and methods using umbilical cord matrix cells
US7150990B2 (en) 2002-03-06 2006-12-19 Reprocell, Inc. Self-renewing pluripotent hepatic stem cells
JP2003259862A (ja) 2002-03-12 2003-09-16 Fuji Photo Film Co Ltd 細胞培養担体
JPWO2003080822A1 (ja) 2002-03-27 2005-07-28 ニプロ株式会社 胎盤由来の間葉系細胞およびその医学的用途
US20050118715A1 (en) 2002-04-12 2005-06-02 Hariri Robert J. Modulation of stem and progenitor cell differentiation, assays, and uses thereof
US7498171B2 (en) 2002-04-12 2009-03-03 Anthrogenesis Corporation Modulation of stem and progenitor cell differentiation, assays, and uses thereof
JP4136434B2 (ja) 2002-04-17 2008-08-20 進 清野 インスリン産生細胞の誘導
US20040161419A1 (en) 2002-04-19 2004-08-19 Strom Stephen C. Placental stem cells and uses thereof
EP1497435A4 (en) 2002-04-19 2005-07-27 Univ Pittsburgh STEM CELLS DERIVED FROM PLAZENTA AND THEIR USES
US20030228295A1 (en) 2002-04-25 2003-12-11 Svendsen Clive N. Use of human neural stem cells secreting GDNF for treatment of parkinson's and other neurodegenerative diseases
US20040029269A1 (en) * 2002-05-07 2004-02-12 Goldman Steven A Promoter-based isolation, purification, expansion, and transplantation of neuronal progenitor cells, oligodendrocyte progenitor cells, or neural stem cells from a population of embryonic stem cells
WO2003094965A2 (en) * 2002-05-08 2003-11-20 Neuronova Ab Modulation of neural stem cells with s1p or lpa receptor agonists
BR0311360A (pt) 2002-05-28 2006-06-06 Becton Dickinson Co métodos para expansão e transdiferenciação in vitro de células acinares pancreáticas humanas em células produtoras de insulina
CA2488013A1 (en) 2002-05-30 2003-12-11 Celgene Corporation Methods of using jnk or mkk inhibitors to modulate cell differentiation and to treat myeloproliferative disorders and myelodysplastic syndromes
WO2003104442A1 (en) 2002-06-07 2003-12-18 Es Cell International Pte Ltd Methods of regulating differentiation in stem cells
US7410797B2 (en) * 2002-06-11 2008-08-12 Ogle Roy C Meningeal-derived stem cells
AU2003279912A1 (en) 2002-06-27 2004-01-19 Northwestern University Peptide rod amphiphiles and self-assembly of same
US7285415B2 (en) * 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
GB0216286D0 (en) 2002-07-15 2002-08-21 Univ Leeds Network
AU2003242976A1 (en) * 2002-07-16 2004-02-02 Yissum Research Development Company Of The Hebrew University Of Jerusalem Methods of implanting mesenchymal stem cells for tissue repair and formation
US7390659B2 (en) 2002-07-16 2008-06-24 The Trustees Of Columbia University In The City Of New York Methods for inducing differentiation of embryonic stem cells and uses thereof
US20040110287A1 (en) 2002-07-29 2004-06-10 Es Cell International Pte Ltd. Multi-step method for the differentiation of insulin positive, glucose responsive cells
AU2003250666A1 (en) 2002-07-29 2004-02-16 Asahi Kasei Kabushiki Kaisha Stem cells for treating pancreatic damage
US20040063204A1 (en) 2002-08-14 2004-04-01 Lijun Yang Bone marrow cell differentiation
AU2003265094A1 (en) 2002-08-26 2004-03-11 Neuronova Ab Method for culturing stem cells
AU2003265856A1 (en) 2002-08-28 2004-03-19 University Of Florida Neurogenesis from hepatic stem cells
JP2005537803A (ja) 2002-09-06 2005-12-15 アムサイト インコーポレーティッド Ｃｄ５６陽性ヒト成体膵臓内分泌前駆細胞
US9969977B2 (en) 2002-09-20 2018-05-15 Garnet Biotherapeutics Cell populations which co-express CD49c and CD90
US6766863B2 (en) * 2002-09-20 2004-07-27 Hypro Corporation Fire fighting foam injection system with auto-start feature
US20040062753A1 (en) 2002-09-27 2004-04-01 Alireza Rezania Composite scaffolds seeded with mammalian cells
JP4262465B2 (ja) * 2002-10-24 2009-05-13 富士フイルム株式会社 細胞培養方法
AU2003287444A1 (en) 2002-10-31 2004-05-25 The General Hospital Corporation Repairing or replacing tissues or organs
US20040258670A1 (en) 2002-12-05 2004-12-23 Case Western Reserve University Cell-based therapies for ischemia
JP4571387B2 (ja) * 2003-02-07 2010-10-27 宣男 櫻川 ヒト羊膜由来サイドポピュレーション細胞及びその用途
AU2004210853A1 (en) 2003-02-11 2004-08-26 Northwestern University Methods and materials for nanocrystalline surface coatings and attachment of peptide amphiphile nanofibers thereon
JP4790592B2 (ja) 2003-02-11 2011-10-12 ダビース，ジヨン・イー ヒト臍帯のウォートンジェリーからの前駆細胞
US7521481B2 (en) * 2003-02-27 2009-04-21 Mclaurin Joanne Methods of preventing, treating and diagnosing disorders of protein aggregation
US20040224401A1 (en) * 2003-03-28 2004-11-11 Ludwig Tenneille E. Physiochemical culture conditions for embryonic stem cells
EP1636351A4 (en) 2003-05-21 2007-04-04 Gen Hospital Corp MICROFABRICATED COMPOSITIONS AND METHODS OF TISSUE GENIUS FOR OBTAINING TISSUES CONTAINING MULTIPLE CELLULAR TYPES
US9572840B2 (en) 2003-06-27 2017-02-21 DePuy Synthes Products, Inc. Regeneration and repair of neural tissue using postpartum-derived cells
ES2552226T3 (es) 2003-06-27 2015-11-26 DePuy Synthes Products, Inc. Reparación y regeneración de cartílago y hueso utilizando células derivadas posparto
US8790637B2 (en) 2003-06-27 2014-07-29 DePuy Synthes Products, LLC Repair and regeneration of ocular tissue using postpartum-derived cells
US20060223177A1 (en) 2003-06-27 2006-10-05 Ethicon Inc. Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
US7875272B2 (en) 2003-06-27 2011-01-25 Ethicon, Incorporated Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells
AU2004269409A1 (en) 2003-08-29 2005-03-10 Regents Of The University Of Minnesota Kidney derived stem cells and methods for their isolation, differentiation and use
US20050089513A1 (en) 2003-10-28 2005-04-28 Norio Sakuragawa Side population cells originated from human amnion and their uses
ATE486540T1 (de) 2003-12-23 2010-11-15 Fmc Biopolymer As Verwendung von alginat-matrizen zur kontrolle des zellwachstums
TWI276685B (en) 2003-12-30 2007-03-21 Ind Tech Res Inst Conditional medium for culturing Schwann cells
US7534606B2 (en) 2004-01-12 2009-05-19 National Health Research Institutes Placental stem cell and methods thereof
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
US8039258B2 (en) * 2004-09-28 2011-10-18 Ethicon, Inc. Tissue-engineering scaffolds containing self-assembled-peptide hydrogels
CA2585547A1 (en) 2004-10-29 2006-05-11 Centocor, Inc. Chemically defined media compositions
US7655678B2 (en) * 2004-11-30 2010-02-02 Council of Scientfic & Industrial Research Pharmaceutical composition for the management of tumors
WO2006101548A2 (en) 2004-12-21 2006-09-28 Ethicon, Inc. Postpartum cells derived from umbilical cord tissue, and methods of making, culturing, and using the same
US20060166361A1 (en) 2004-12-21 2006-07-27 Agnieszka Seyda Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same
US20060153815A1 (en) 2004-12-21 2006-07-13 Agnieszka Seyda Tissue engineering devices for the repair and regeneration of tissue
CA2592435C (en) 2004-12-23 2017-03-28 DePuy Synthes Products, Inc. Treatment of stroke and other acute neural degenerative disorders using postpartum derived cells
WO2006071773A2 (en) 2004-12-23 2006-07-06 Ethicon Incoporated Treatment of osteochondral diseases using postpartum-derived cells and products thereof
EP1831355A2 (en) 2004-12-23 2007-09-12 Ethicon, Inc. Soft tissue repair and regeneration using postpartum-derived cells and cell products
US7741311B2 (en) 2005-01-03 2010-06-22 Shaker Mousa Composition and method for treating occlusive vascular diseases, nerve regeneration, and wound healing
EP1853698A1 (en) 2005-01-28 2007-11-14 NovaThera Ltd. Methods for embryonic stem cell culture
CN103361302A (zh) 2005-03-31 2013-10-23 斯丹姆涅恩有限公司 从高度纯化来自羊膜的细胞群获得的细胞裂解产物的组合物
US7923007B2 (en) 2005-08-08 2011-04-12 Academia Sinica Brain tissue damage therapies
JP5289970B2 (ja) 2005-12-16 2013-09-11 エシコン・インコーポレイテッド 組織適合性不適合な移植における逆免疫反応を抑制するための組成物および方法
AU2006327073B2 (en) 2005-12-19 2012-08-30 Ethicon, Inc. In vitro expansion of postpartum derived cells in roller bottles
US9125906B2 (en) 2005-12-28 2015-09-08 DePuy Synthes Products, Inc. Treatment of peripheral vascular disease using umbilical cord tissue-derived cells
EP1979050B1 (en) 2005-12-28 2017-04-19 DePuy Synthes Products, Inc. Treatment of peripheral vascular disease using postpartum-derived cells
ES2671355T3 (es) 2005-12-29 2018-06-06 Anthrogenesis Corporation Poblaciones de células madre placentarias
JP5106416B2 (ja) * 2006-01-06 2012-12-26 アジレント・テクノロジーズ・インク 濃縮（ｐａｃｋｅｄ）ＤＮＡポリメラーゼを含む核酸複製のための反応緩衝液組成物
ES2549528T3 (es) 2006-03-23 2015-10-29 Pluristem Ltd. Métodos de expansión de células y usos de células y de medios de acondicionamiento producidos por los mismos para terapia
WO2007146105A2 (en) * 2006-06-05 2007-12-21 Cryo-Cell International, Inc. Procurement, isolation and cryopreservation of fetal placental cells
DK2078073T3 (da) 2006-10-12 2013-10-28 Ethicon Inc Nyreafledte celler og fremgangsmåder til anvendelse deraf til vævsreparation og -regenerering
EP2089511B1 (en) 2006-11-13 2014-09-17 DePuy Synthes Products, LLC In vitro expansion of postpartum-derived cells using microcarriers
SI2120977T1 (sl) 2007-02-12 2014-01-31 Anthrogenesis Coroporation Zdravljenje vnetnih bolezni z uporabo placentarnih matičnih celic
US20080305148A1 (en) 2007-03-19 2008-12-11 National Yang Ming University Treatment of spinal injuries using human umbilical mesenchymal stem cells
CA2701435C (en) 2007-10-05 2017-06-20 Ethicon, Inc. Repair and regeneration of renal tissue using human umbilical cord tissue-derived cells
US20090123620A1 (en) * 2007-11-14 2009-05-14 Hiti Thomas R Automated Application of an Antimycotic Composition to Sliced Foodstuffs and an Antimycotic Application Apparatus
US8236538B2 (en) 2007-12-20 2012-08-07 Advanced Technologies And Regenerative Medicine, Llc Methods for sterilizing materials containing biologically active agents
US20090186358A1 (en) * 2007-12-21 2009-07-23 Wyeth Pathway Analysis of Cell Culture Phenotypes and Uses Thereof
ES2621610T3 (es) 2007-12-27 2017-07-04 DePuy Synthes Products, Inc. Tratamiento de la degeneración de discos intervertebrales utilizando células derivadas de tejido cordón umbilical humano
TWI387135B (zh) * 2008-03-28 2013-02-21 財團法人工業技術研究院 發光裝置及其製造方法
AU2009327383B2 (en) 2008-12-19 2014-08-28 DePuy Synthes Products, LLC Regeneration and repair of neural tissue following injury
CN107028983A (zh) 2008-12-19 2017-08-11 德普伊新特斯产品有限责任公司 肺部疾病和病症的治疗
US10179900B2 (en) 2008-12-19 2019-01-15 DePuy Synthes Products, Inc. Conditioned media and methods of making a conditioned media
JP5646502B2 (ja) 2008-12-19 2014-12-24 デピュイ・シンセス・プロダクツ・エルエルシーＤｅＰｕｙ Ｓｙｎｔｈｅｓ Ｐｒｏｄｕｃｔｓ， ＬＬＣ 神経因性疼痛を治療するための臍帯組織由来細胞
US8722034B2 (en) 2009-03-26 2014-05-13 Depuy Synthes Products Llc hUTC as therapy for Alzheimer's disease
KR20140018876A (ko) * 2011-01-19 2014-02-13 이 아이 듀폰 디 네모아 앤드 캄파니 폐쇄 기능을 가진 리튬 배터리 격리판

Also Published As

Publication number Publication date
CA2530732A1 (en) 2005-01-06
US8703121B2 (en) 2014-04-22
US20070014771A1 (en) 2007-01-18
CA2530416C (en) 2015-08-25
US7413734B2 (en) 2008-08-19
EP2399990A1 (en) 2011-12-28
EP2341131A2 (en) 2011-07-06
ES2569780T3 (es) 2016-05-12
PL1649013T3 (pl) 2016-07-29
EP1641914B1 (en) 2016-07-20
US20070009494A1 (en) 2007-01-11
US9717763B2 (en) 2017-08-01
US8277796B2 (en) 2012-10-02
US20190192577A1 (en) 2019-06-27
EP2338982A3 (en) 2011-08-03
JP2007528703A (ja) 2007-10-18
JP4950661B2 (ja) 2012-06-13
AU2004254616B2 (en) 2011-09-01
EP2399990B1 (en) 2015-07-22
JP2007521009A (ja) 2007-08-02
ES2568463T3 (es) 2016-04-29
EP2341131A3 (en) 2011-08-03
CA2530422C (en) 2016-01-05
EP1641916A2 (en) 2006-04-05
EP1649013B1 (en) 2016-01-20
US20170326183A1 (en) 2017-11-16
US8658152B2 (en) 2014-02-25
WO2005001076A3 (en) 2005-03-31
WO2005001078A3 (en) 2005-04-21
US11000554B2 (en) 2021-05-11
ES2564044T3 (es) 2016-03-17
ES2582342T3 (es) 2016-09-12
US20060153816A1 (en) 2006-07-13
JP2007521793A (ja) 2007-08-09
AU2004252568B2 (en) 2011-06-30
AU2004254616C1 (en) 2012-03-01
AU2004252568A1 (en) 2005-01-06
US20050019865A1 (en) 2005-01-27
EP1641917B1 (en) 2016-04-27
EP2322599A3 (en) 2011-07-27
US20050054098A1 (en) 2005-03-10
WO2005001080A3 (en) 2005-03-24
US10220059B2 (en) 2019-03-05
US10500234B2 (en) 2019-12-10
EP2322599A2 (en) 2011-05-18
CA2530421A1 (en) 2005-01-06
US20170166863A1 (en) 2017-06-15
ES2542070T3 (es) 2015-07-30
EP2341131B1 (en) 2015-08-05
ES2564045T3 (es) 2016-03-17
US11191789B2 (en) 2021-12-07
US20180338999A1 (en) 2018-11-29
AU2004252570C1 (en) 2012-03-01
AU2004281371A1 (en) 2005-04-28
ES2600555T3 (es) 2017-02-09
EP1641913A2 (en) 2006-04-05
AU2004252567A1 (en) 2005-01-06
JP2007528706A (ja) 2007-10-18
US20100260843A1 (en) 2010-10-14
CA2530412A1 (en) 2005-04-28
US20050058630A1 (en) 2005-03-17
EP2336298B1 (en) 2016-02-17
ES2550267T3 (es) 2015-11-05
EP1641913B1 (en) 2016-01-06
US20140045263A1 (en) 2014-02-13
JP2007528702A (ja) 2007-10-18
EP1641914A2 (en) 2006-04-05
WO2005001077A2 (en) 2005-01-06
US9579351B2 (en) 2017-02-28
CA2530255C (en) 2015-12-08
ES2542069T3 (es) 2015-07-30
PL2341131T3 (pl) 2016-01-29
AU2004281371C1 (en) 2012-10-11
US9498501B2 (en) 2016-11-22
AU2004254616A1 (en) 2005-01-13
WO2005038012A3 (en) 2005-09-15
AU2004252571A1 (en) 2005-01-06
WO2005003334A3 (en) 2005-04-07
JP5148873B2 (ja) 2013-02-20
PL2338980T3 (pl) 2016-04-29
US20170143770A1 (en) 2017-05-25
AU2004252571B2 (en) 2011-09-15
EP2338981A3 (en) 2011-08-03
US10195233B2 (en) 2019-02-05
JP2007521008A (ja) 2007-08-02
JP4950659B2 (ja) 2012-06-13
PL1641914T3 (pl) 2017-01-31
CA2530416A1 (en) 2005-01-06
PL2338981T3 (pl) 2016-01-29
AU2004252571C1 (en) 2012-03-01
EP1641915A2 (en) 2006-04-05
EP1649013A2 (en) 2006-04-26
US8318483B2 (en) 2012-11-27
EP2336298A3 (en) 2011-08-03
CA2530533C (en) 2015-02-10
ES2554343T3 (es) 2015-12-18
EP2338980A3 (en) 2011-08-10
PL1641918T3 (pl) 2016-06-30
US20050037491A1 (en) 2005-02-17
US20050058629A1 (en) 2005-03-17
US20060188983A1 (en) 2006-08-24
JP2007528705A (ja) 2007-10-18
US20060153818A1 (en) 2006-07-13
EP2336298A2 (en) 2011-06-22
AU2004281371B2 (en) 2012-05-10
CA2530412C (en) 2016-02-09
AU2004252570B2 (en) 2011-09-15
EP1641915B1 (en) 2016-07-27
EP2338981B1 (en) 2015-04-22
JP4948164B2 (ja) 2012-06-06
WO2005038012A2 (en) 2005-04-28
PL2399990T3 (pl) 2015-12-31
WO2005003334A2 (en) 2005-01-13
WO2005001080A2 (en) 2005-01-06
EP2322599B1 (en) 2015-04-15
EP2338980A2 (en) 2011-06-29
PL2322599T3 (pl) 2015-10-30
US20060153817A1 (en) 2006-07-13
AU2004252570A1 (en) 2005-01-06
US10039793B2 (en) 2018-08-07
US20070036767A1 (en) 2007-02-15
EP1641918A2 (en) 2006-04-05
US20050058631A1 (en) 2005-03-17
US20170166864A1 (en) 2017-06-15
US20130022585A1 (en) 2013-01-24
WO2005001076A2 (en) 2005-01-06
PL1641917T3 (pl) 2016-11-30
EP2338980B1 (en) 2015-04-22
JP4948166B2 (ja) 2012-06-06
JP4950660B2 (ja) 2012-06-13
ES2541604T3 (es) 2015-07-22
WO2005001078A2 (en) 2005-01-06
EP2338982B1 (en) 2015-08-26
US20060234376A1 (en) 2006-10-19
EP1641917A2 (en) 2006-04-05
ES2565582T3 (es) 2016-04-05
CA2530421C (en) 2015-04-21
PL1641913T3 (pl) 2016-06-30
US7524489B2 (en) 2009-04-28
AU2004252567B2 (en) 2011-10-06
US20050032209A1 (en) 2005-02-10
EP1641916B1 (en) 2016-02-17
CA2530255A1 (en) 2005-01-06
WO2005001079A2 (en) 2005-01-06
AU2004252566B2 (en) 2011-09-15
US7560276B2 (en) 2009-07-14
EP2338982A2 (en) 2011-06-29
PL2338982T3 (pl) 2016-01-29
CA2530422A1 (en) 2005-01-06
US20060154367A1 (en) 2006-07-13
US9504719B2 (en) 2016-11-29
PL1641915T3 (pl) 2017-01-31
JP4948165B2 (ja) 2012-06-06
CA2530533A1 (en) 2005-01-13
US20060154366A1 (en) 2006-07-13
ES2597837T3 (es) 2017-01-23
US10758576B2 (en) 2020-09-01
US7510873B2 (en) 2009-03-31
WO2005001079A3 (en) 2005-04-28
US20170239299A1 (en) 2017-08-24
PL1641916T3 (pl) 2016-08-31
CA2530732C (en) 2015-03-31
EP2338981A2 (en) 2011-06-29
US10383898B2 (en) 2019-08-20
WO2005001077A3 (en) 2005-03-03
AU2004252566A1 (en) 2005-01-06
PL2336298T3 (pl) 2016-08-31
EP1641918B1 (en) 2016-01-06
US20100210013A1 (en) 2010-08-19
US11179422B2 (en) 2021-11-23

Similar Documents

Publication Publication Date Title
ES2552226T3 (es) Reparación y regeneración de cartílago y hueso utilizando células derivadas posparto
JP5289970B2 (ja) 組織適合性不適合な移植における逆免疫反応を抑制するための組成物および方法
ES2621847T3 (es) Células posparto derivadas de tejido de cordón umbilical, y métodos de elaboración y uso de las mismas
EP2237789B1 (en) Treatment of intervertebral disc degeneration using human umbilical cord tissue-derived cells
CA2747727C (en) Conditioned medium and methods of making the same
EP2379087B1 (en) Umbilical cord tissue derived cells for treating neuropathic pain and spasticity
US20060171930A1 (en) Postpartum cells derived from umbilical cord tissue, and methods of making, culturing, and using the same
US20190105352A9 (en) Treatment of intervertebral disc degeneration using human umbilical cord tissue-derived cells