JPH0124800B2 - - Google Patents
Info
- Publication number
- JPH0124800B2 JPH0124800B2 JP50125830A JP12583075A JPH0124800B2 JP H0124800 B2 JPH0124800 B2 JP H0124800B2 JP 50125830 A JP50125830 A JP 50125830A JP 12583075 A JP12583075 A JP 12583075A JP H0124800 B2 JPH0124800 B2 JP H0124800B2
- Authority
- JP
- Japan
- Prior art keywords
- astrocytin
- malignin
- tag
- recognin
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108010090946 astrocytin Proteins 0.000 claims description 90
- 108010031052 malignin Proteins 0.000 claims description 90
- 206010028980 Neoplasm Diseases 0.000 claims description 66
- 102000004169 proteins and genes Human genes 0.000 claims description 61
- 108090000623 proteins and genes Proteins 0.000 claims description 61
- 239000000126 substance Substances 0.000 claims description 59
- 230000007935 neutral effect Effects 0.000 claims description 25
- 238000004519 manufacturing process Methods 0.000 claims description 23
- 239000000284 extract Substances 0.000 claims description 16
- -1 bromoacetyl Chemical group 0.000 claims description 13
- 239000000872 buffer Substances 0.000 claims description 11
- 239000001913 cellulose Substances 0.000 claims description 9
- 229920002678 cellulose Polymers 0.000 claims description 9
- 229920001184 polypeptide Polymers 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 230000002797 proteolythic effect Effects 0.000 claims 2
- 230000000536 complexating effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 description 72
- 239000000047 product Substances 0.000 description 69
- 210000004027 cell Anatomy 0.000 description 68
- 239000000243 solution Substances 0.000 description 62
- 235000018102 proteins Nutrition 0.000 description 59
- 210000002966 serum Anatomy 0.000 description 52
- 210000001519 tissue Anatomy 0.000 description 45
- 238000012360 testing method Methods 0.000 description 39
- 201000011510 cancer Diseases 0.000 description 36
- 239000003153 chemical reaction reagent Substances 0.000 description 26
- 210000004881 tumor cell Anatomy 0.000 description 26
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 24
- 208000003174 Brain Neoplasms Diseases 0.000 description 23
- 239000002243 precursor Substances 0.000 description 23
- 239000000499 gel Substances 0.000 description 21
- 210000004556 brain Anatomy 0.000 description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 20
- 238000010521 absorption reaction Methods 0.000 description 19
- 239000000463 material Substances 0.000 description 18
- 150000001875 compounds Chemical class 0.000 description 17
- 239000006228 supernatant Substances 0.000 description 17
- 241000124008 Mammalia Species 0.000 description 16
- 102000006395 Globulins Human genes 0.000 description 15
- 108010044091 Globulins Proteins 0.000 description 15
- 230000001965 increasing effect Effects 0.000 description 15
- 235000001014 amino acid Nutrition 0.000 description 14
- 229940024606 amino acid Drugs 0.000 description 14
- 230000012010 growth Effects 0.000 description 14
- 238000000338 in vitro Methods 0.000 description 14
- 108010068258 recognins Proteins 0.000 description 14
- 150000001413 amino acids Chemical class 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 13
- 239000002253 acid Substances 0.000 description 12
- 210000001124 body fluid Anatomy 0.000 description 12
- 239000010839 body fluid Substances 0.000 description 12
- 239000000203 mixture Substances 0.000 description 11
- 239000002244 precipitate Substances 0.000 description 11
- 239000011347 resin Substances 0.000 description 11
- 229920005989 resin Polymers 0.000 description 11
- 208000032612 Glial tumor Diseases 0.000 description 10
- 206010018338 Glioma Diseases 0.000 description 10
- 230000002378 acidificating effect Effects 0.000 description 10
- 239000000427 antigen Substances 0.000 description 10
- 102000036639 antigens Human genes 0.000 description 10
- 108091007433 antigens Proteins 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 239000007853 buffer solution Substances 0.000 description 9
- 239000003795 chemical substances by application Substances 0.000 description 9
- 238000004587 chromatography analysis Methods 0.000 description 9
- 239000012153 distilled water Substances 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 235000004252 protein component Nutrition 0.000 description 9
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 8
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 8
- 241000283973 Oryctolagus cuniculus Species 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 239000007864 aqueous solution Substances 0.000 description 8
- 230000001413 cellular effect Effects 0.000 description 8
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 8
- 238000012817 gel-diffusion technique Methods 0.000 description 8
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 8
- 235000014304 histidine Nutrition 0.000 description 8
- 239000002904 solvent Substances 0.000 description 8
- 230000003595 spectral effect Effects 0.000 description 8
- 230000008685 targeting Effects 0.000 description 8
- 238000005406 washing Methods 0.000 description 8
- CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 7
- 238000005119 centrifugation Methods 0.000 description 7
- 239000006185 dispersion Substances 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 239000008363 phosphate buffer Substances 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 238000009482 thermal adhesion granulation Methods 0.000 description 7
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 6
- 235000003704 aspartic acid Nutrition 0.000 description 6
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 230000032823 cell division Effects 0.000 description 6
- 238000003776 cleavage reaction Methods 0.000 description 6
- 231100000135 cytotoxicity Toxicity 0.000 description 6
- 230000003013 cytotoxicity Effects 0.000 description 6
- 239000000975 dye Substances 0.000 description 6
- 238000010828 elution Methods 0.000 description 6
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 6
- 238000005227 gel permeation chromatography Methods 0.000 description 6
- 235000013922 glutamic acid Nutrition 0.000 description 6
- 239000004220 glutamic acid Substances 0.000 description 6
- 230000036210 malignancy Effects 0.000 description 6
- 230000003211 malignant effect Effects 0.000 description 6
- 210000004498 neuroglial cell Anatomy 0.000 description 6
- 210000000056 organ Anatomy 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 6
- 230000007017 scission Effects 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 239000004475 Arginine Substances 0.000 description 5
- 108010052832 Cytochromes Proteins 0.000 description 5
- 102000018832 Cytochromes Human genes 0.000 description 5
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 5
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 5
- 102000004142 Trypsin Human genes 0.000 description 5
- 108090000631 Trypsin Proteins 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 235000004279 alanine Nutrition 0.000 description 5
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 5
- 235000009697 arginine Nutrition 0.000 description 5
- 239000013060 biological fluid Substances 0.000 description 5
- 230000004663 cell proliferation Effects 0.000 description 5
- 239000000356 contaminant Substances 0.000 description 5
- 238000003745 diagnosis Methods 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 238000010166 immunofluorescence Methods 0.000 description 5
- 238000001727 in vivo Methods 0.000 description 5
- 238000002955 isolation Methods 0.000 description 5
- 229930182817 methionine Natural products 0.000 description 5
- 235000006109 methionine Nutrition 0.000 description 5
- 230000000717 retained effect Effects 0.000 description 5
- 230000001225 therapeutic effect Effects 0.000 description 5
- 231100000331 toxic Toxicity 0.000 description 5
- 230000002588 toxic effect Effects 0.000 description 5
- 239000012588 trypsin Substances 0.000 description 5
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 description 4
- VEVRNHHLCPGNDU-MUGJNUQGSA-N (2s)-2-amino-5-[1-[(5s)-5-amino-5-carboxypentyl]-3,5-bis[(3s)-3-amino-3-carboxypropyl]pyridin-1-ium-4-yl]pentanoate Chemical compound OC(=O)[C@@H](N)CCCC[N+]1=CC(CC[C@H](N)C(O)=O)=C(CCC[C@H](N)C([O-])=O)C(CC[C@H](N)C(O)=O)=C1 VEVRNHHLCPGNDU-MUGJNUQGSA-N 0.000 description 4
- 229920000298 Cellophane Polymers 0.000 description 4
- 239000004471 Glycine Substances 0.000 description 4
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 4
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 4
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 4
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 4
- LRQKBLKVPFOOQJ-YFKPBYRVSA-N L-norleucine Chemical compound CCCC[C@H]([NH3+])C([O-])=O LRQKBLKVPFOOQJ-YFKPBYRVSA-N 0.000 description 4
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 4
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 4
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 4
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 4
- 239000004472 Lysine Substances 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 4
- 102000035195 Peptidases Human genes 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 4
- 229920005654 Sephadex Polymers 0.000 description 4
- 239000012507 Sephadex⢠Substances 0.000 description 4
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 4
- 239000004473 Threonine Substances 0.000 description 4
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 4
- 229910021529 ammonia Inorganic materials 0.000 description 4
- 239000012062 aqueous buffer Substances 0.000 description 4
- 210000005013 brain tissue Anatomy 0.000 description 4
- 239000006143 cell culture medium Substances 0.000 description 4
- 210000001638 cerebellum Anatomy 0.000 description 4
- 231100000433 cytotoxic Toxicity 0.000 description 4
- 230000001472 cytotoxic effect Effects 0.000 description 4
- 238000000502 dialysis Methods 0.000 description 4
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 229960003692 gamma aminobutyric acid Drugs 0.000 description 4
- 230000003301 hydrolyzing effect Effects 0.000 description 4
- 229960002591 hydroxyproline Drugs 0.000 description 4
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 4
- 229960000310 isoleucine Drugs 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 4
- 235000002374 tyrosine Nutrition 0.000 description 4
- 239000004474 valine Substances 0.000 description 4
- HYLBTMZBXLEVCL-UWVGGRQHSA-N (2s)-2-amino-6-[[(5s)-5-amino-5-carboxypentyl]amino]hexanoic acid Chemical compound OC(=O)[C@@H](N)CCCCNCCCC[C@H](N)C(O)=O HYLBTMZBXLEVCL-UWVGGRQHSA-N 0.000 description 3
- 108010075028 Cytochromes b Proteins 0.000 description 3
- 102000003886 Glycoproteins Human genes 0.000 description 3
- 108090000288 Glycoproteins Proteins 0.000 description 3
- LCWXJXMHJVIJFK-UHFFFAOYSA-N Hydroxylysine Natural products NCC(O)CC(N)CC(O)=O LCWXJXMHJVIJFK-UHFFFAOYSA-N 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 125000000539 amino acid group Chemical group 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 201000007983 brain glioma Diseases 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 3
- 235000018417 cysteine Nutrition 0.000 description 3
- YSMODUONRAFBET-UHFFFAOYSA-N delta-DL-hydroxylysine Natural products NCC(O)CCC(N)C(O)=O YSMODUONRAFBET-UHFFFAOYSA-N 0.000 description 3
- 238000002405 diagnostic procedure Methods 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- YSMODUONRAFBET-UHNVWZDZSA-N erythro-5-hydroxy-L-lysine Chemical compound NC[C@H](O)CC[C@H](N)C(O)=O YSMODUONRAFBET-UHNVWZDZSA-N 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 150000003278 haem Chemical class 0.000 description 3
- QJHBJHUKURJDLG-UHFFFAOYSA-N hydroxy-L-lysine Natural products NCCCCC(NO)C(O)=O QJHBJHUKURJDLG-UHFFFAOYSA-N 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- RGXCTRIQQODGIZ-UHFFFAOYSA-O isodesmosine Chemical compound OC(=O)C(N)CCCC[N+]1=CC(CCC(N)C(O)=O)=CC(CCC(N)C(O)=O)=C1CCCC(N)C(O)=O RGXCTRIQQODGIZ-UHFFFAOYSA-O 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 108010057846 lysinenorleucine Proteins 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 230000005012 migration Effects 0.000 description 3
- 210000000653 nervous system Anatomy 0.000 description 3
- 210000002569 neuron Anatomy 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000002285 radioactive effect Effects 0.000 description 3
- 239000000941 radioactive substance Substances 0.000 description 3
- 230000000241 respiratory effect Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000004809 thin layer chromatography Methods 0.000 description 3
- LCTORNIWLGOBPB-GASJEMHNSA-N (3r,4s,5s,6r)-2-amino-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound NC1(O)O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O LCTORNIWLGOBPB-GASJEMHNSA-N 0.000 description 2
- HTCSFFGLRQDZDE-UHFFFAOYSA-N 2-azaniumyl-2-phenylpropanoate Chemical compound OC(=O)C(N)(C)C1=CC=CC=C1 HTCSFFGLRQDZDE-UHFFFAOYSA-N 0.000 description 2
- 239000000592 Artificial Cell Substances 0.000 description 2
- 108010017384 Blood Proteins Proteins 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 2
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 102100025287 Cytochrome b Human genes 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 108010062374 Myoglobin Proteins 0.000 description 2
- 102100030856 Myoglobin Human genes 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 230000005875 antibody response Effects 0.000 description 2
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 2
- 229910052794 bromium Inorganic materials 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- XVOYSCVBGLVSOL-UHFFFAOYSA-N cysteic acid Chemical compound OC(=O)C(N)CS(O)(=O)=O XVOYSCVBGLVSOL-UHFFFAOYSA-N 0.000 description 2
- 229960003067 cystine Drugs 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 229920001971 elastomer Polymers 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000002518 glial effect Effects 0.000 description 2
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000000703 high-speed centrifugation Methods 0.000 description 2
- 238000000265 homogenisation Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000013541 low molecular weight contaminant Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 2
- 235000019799 monosodium phosphate Nutrition 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Chemical compound OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 239000007981 phosphate-citrate buffer Substances 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000000163 radioactive labelling Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 2
- FYKDNWHPKQOZOT-UHFFFAOYSA-M sodium;dihydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].OP(O)([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O FYKDNWHPKQOZOT-UHFFFAOYSA-M 0.000 description 2
- 238000000527 sonication Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 229940124597 therapeutic agent Drugs 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- LCTORNIWLGOBPB-PHYPRBDBSA-N (2s,3r,4s,5r,6r)-2-amino-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound N[C@@]1(O)O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O LCTORNIWLGOBPB-PHYPRBDBSA-N 0.000 description 1
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 1
- UEUIKXVPXLWUDU-UHFFFAOYSA-N 4-diazoniobenzenesulfonate Chemical compound [O-]S(=O)(=O)C1=CC=C([N+]#N)C=C1 UEUIKXVPXLWUDU-UHFFFAOYSA-N 0.000 description 1
- OCKGFTQIICXDQW-ZEQRLZLVSA-N 5-[(1r)-1-hydroxy-2-[4-[(2r)-2-hydroxy-2-(4-methyl-1-oxo-3h-2-benzofuran-5-yl)ethyl]piperazin-1-yl]ethyl]-4-methyl-3h-2-benzofuran-1-one Chemical compound C1=C2C(=O)OCC2=C(C)C([C@@H](O)CN2CCN(CC2)C[C@H](O)C2=CC=C3C(=O)OCC3=C2C)=C1 OCKGFTQIICXDQW-ZEQRLZLVSA-N 0.000 description 1
- 101710146995 Acyl carrier protein Proteins 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 102000006410 Apoproteins Human genes 0.000 description 1
- 108010083590 Apoproteins Proteins 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 1
- 206010018341 Gliosis Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 208000017604 Hodgkin disease Diseases 0.000 description 1
- 208000010747 Hodgkins lymphoma Diseases 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 238000003231 Lowry assay Methods 0.000 description 1
- 238000009013 Lowry's assay Methods 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 102000011931 Nucleoproteins Human genes 0.000 description 1
- 108010061100 Nucleoproteins Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000012287 Prolapse Diseases 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 101000857870 Squalus acanthias Gonadoliberin Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 235000003591 Tagetes lucida Nutrition 0.000 description 1
- 240000002670 Tagetes lucida Species 0.000 description 1
- 208000024770 Thyroid neoplasm Diseases 0.000 description 1
- 101710100170 Unknown protein Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- ZCHPKWUIAASXPV-UHFFFAOYSA-N acetic acid;methanol Chemical compound OC.CC(O)=O ZCHPKWUIAASXPV-UHFFFAOYSA-N 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 150000005829 chemical entities Chemical class 0.000 description 1
- 239000012707 chemical precursor Substances 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 230000001447 compensatory effect Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000001268 conjugating effect Effects 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 239000012156 elution solvent Substances 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 230000007387 gliosis Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 235000004554 glutamine Nutrition 0.000 description 1
- XLXSAKCOAKORKW-AQJXLSMYSA-N gonadorelin Chemical compound C([C@@H](C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 XLXSAKCOAKORKW-AQJXLSMYSA-N 0.000 description 1
- 229940035638 gonadotropin-releasing hormone Drugs 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 238000010562 histological examination Methods 0.000 description 1
- 125000001183 hydrocarbyl group Chemical group 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000007917 intracranial administration Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000000622 irritating effect Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 210000000944 nerve tissue Anatomy 0.000 description 1
- 238000000424 optical density measurement Methods 0.000 description 1
- 201000008968 osteosarcoma Diseases 0.000 description 1
- 238000013021 overheating Methods 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000005195 poor health Effects 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 230000022558 protein metabolic process Effects 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000004202 respiratory function Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 239000008174 sterile solution Substances 0.000 description 1
- 238000012414 sterilization procedure Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 235000013706 tagetes lucida Nutrition 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 201000002510 thyroid cancer Diseases 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 238000004454 trace mineral analysis Methods 0.000 description 1
- 125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 description 1
- 230000004565 tumor cell growth Effects 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
æ¬çºæã¯æ°èŠãªååç©ïŒä»¥äžãæ¬æçŽ°æžã§ã¯ã¬
ã³ã°ãã³ïŒRecogninsïŒãšç§°ããïŒã«é¢ãããã¬
ã³ã°ãã³ã¯ãéåžžã®ãŸãã¯ç°åžžãªçްèãŸãã¯çµ
ç¹ãäŸãã°è «ç现èãŸãã¯äººçºçãªç现èãåŠç
ããææã®ç©è³ªãåé¢ããŠè£œãããããã¬ã³ã°ã
ã³ã¯ããããã®ååŠé¢é£ç©è³ªã補ããããã«äœæ¶²
ãæããæ¯æç©ã«ã¬ã³ã°ãã³ãæ¥è§Šããããšã«äœ¿
çšããããšãã§ããããããã®ååŠé¢é£ç©è³ªã¯ã
蚺æããã³æ²»çç®çããªãã¡çã蚺æãããæ²»ç
ããã®ã«æçšãªãã®ã§ããããã®ååŠé¢é£ç©è³ª
ã¯ãçäœäžãããã¯è©Šéšç®¡äžãäŸãã°å®éçæ²
éããªãŒããããã€ïŒOuchterlonyïŒè€æ¡æ£ãã
ãã¯å ç«è¢å æ§ã«ãããŠå ç«ååŠçç¹æ§ã§ã¬ã³ã°
ãã³ãšåå¿ããç©è³ªã§ããã æ¬çºæã«ãããã¬ã³ã°ãã³ã®ïŒã€ã¯ã¢ã¹ããã·
ãã³ïŒAstrocytinïŒã§ãããã¢ã¹ããã·ãã³ã¯è³
è «ççµç¹ã奜ãŸããã¯è³ç¥çµè è «çµç¹ããåŸãã
ããã¢ã¹ããã·ãã³åå ç©ãå«ãèçœè³ªæåã¯æ
åã¯çްèçµç¹ããæœåºãããã奜ãŸããæœåºæ¹æ³
ã¯ãã¢ã¹ããã·ãã³åé§ç©ãå«ãèçœè³ªæåãæº¶
è§£ããããã«ãå質æ¡ä»¶äžã«äžæ§ç·©è¡ç©ãšçµç¹ãš
ãåŠçããããããã¯çްèããã³çµç¹ãåè§£ãã
ããšã§ããã ãã®ç¹ã§ã¢ã¹ããã·ãã³åé§ç©ã¯ãŸã ãèçœ
質ãç³èçœè³ªãèèçœè³ªãæ žé žãæ žèçœè³ªçãå«
ãååéã®å€§ããªç©è³ªã§ããããã®åŸæº¶è§£ããè
çœè³ªã¯çæããçµç¹æœåºç©ããåé¢ããããçµç¹
ããã®æœåºæº¶æ¶²ã¯ç²Ÿè£œããäžæº¶æ§ç²åãé€å»ãã
ããååéã®å°ããæ±æç©ã¯ãåŸãããæº¶æ¶²ãã
èžçºæ¿çž®æè¡ã«ãã€ãŠé€å»ããããåŸãããæº¶æ¶²
ã¯ãã®åŸåŠçãããïŒãïŒã®ç¯å²ã®pKãæãã
èçœè³ªãåŸãããã«ä»ã®æ±æç©ããã¢ã¹ããã·ã
ã³åå ç©ãåè§£ãããããã®åŸããã®æº¶æ¶²ã¯äŸã
ã°ã¯ãããã°ã©ãã€ã«ã©ã ã«èŒçœ®ãããé žæ§æº¶åª
ã§æº¶é¢ããããäžæ§ãŸãã¯pK4以äžã®é žã§æº¶é¢ã
ããèçœè³ªæåã¯ãã¹ãŠæšãŠãããïŒãïŒç¯å²ã®
pKãæãããããã®èçœè³ªæåã¯åéãããã
æº¶åºæ¶²ã¯ãã®åŸåŠçãããçŽ8000ã®ååéãæã
ãç©è³ªãåŸããããããã¯äŸãã°ãææããŸãã
ã€ã«ã¿ãŒã«ãããŠãååéã®å°ããç©è³ªããªãã¡
ååé1000以äžã®ç©è³ªãå»ããå床ãã€ã«ã¿ãŒ
ã«ãããŠçŽ25000以äžã®ç©è³ªãå»ããŠéæãã
ãã1000ã25000ã®ç¯å²ã®ååéãæããèçœè³ª
æåã¯ãã®åŸããã«èå±€ã²ã«ïŒTLGïŒã¯ããã
ã°ã©ãã€ã«ãã€ãŠåŠçãããã¢ã¹ããã·ãã³ãåŸ
ãããã ãã®ããã«ã¢ã¹ããã·ãã³ã¯ãåäžããã³é«é
ã®é å¿åé¢ãããè¿ãããšã«ãã€ãŠè³ç¥çµè è «ã
äžæ§ç·©è¡ç©ã§æœåºããåŸãããæœåºç©ããçŽïŒã
ïŒã®ç¯å²ã®pKãæããèçœè³ªæåãåé¢ããã
ã®èçœè³ªæåããé«ååéããªãã¡çŽ230000以äž
ã®ååéãæããç©è³ªãåé¢ããããããçŽ8000
ã®ååéãæããã¢ã¹ããã·ãã³ãåé¢ããããš
ã«ãã€ãŠã補ããããšãã§ããã äžèšã®æ¹æ³ã«ãã€ãŠè£œããããã¢ã¹ããã·ãã³
ã¯ãå®éçæ²éçŽ ãã¹ãããã³ãªãŒããããã€ã²
ã«æ¡æ£ãã¹ãã«ãããŠç¹æã®æäœãæããåäžç·
ã®æ²éç©ã圢æããé žæ§ãŸãã¯äžæ§PHãæããæ°Ž
溶液ããã³æ°Žææº¶æ¶²ã«å¯æº¶ã§ãããã¢ã«ã«ãªæ§PH
ã§ã¯é£æº¶ã§ãããåå å 床èšã«ããåžåããŒã¯æ³¢
é·ã280ïœÎŒã§ãããçŽ8000ã®ååéãæããŠã
ãããšãç¹åŸŽãšããŠããã ã¢ã¹ããã·ãã³ã®ç¹åŸŽã¯ãŸããã°ã«ã¿ãã³é žã
ãã³ã¢ã¹ãã©ã®ã³é žã®æ®æž£ã®å²åãããããŠé«
ãããã¹ããžã³ã«å¯Ÿãããããã®é žã®å²åããã
ããŠé«ãããšã§ãããããã«ã¢ã¹ããã·ãã³ã®å
æã以äžã«ç€ºãã äžèšãšåæ§ã«ããŠãããªã°ãã³ïŒMaligninïŒ
ãšç§°ããããä»ã®ã¬ã³ã°ãã³ã¯ã人çºçãªç现è
ããªãã¡è©Šéšç®¡äžã§åºæ¿ãåããŠæé·ããç现è
ãã補ãããããããªã°ãã³ã¯ãçŽ10000ã®åå
éãæããã¢ã¹ããã·ãã³ãšã»ãŒé¡äŒŒããŠããã
ã¢ããé žæ®æž£æåãç°ãªããããªãã¡ããªã°ãã³
ã¯ãã°ã«ã¿ãã³é žããã³ã¢ã¹ãã©ã®ã³é žã®å²åã
é«ããã€ãã¹ããžã³ã«å¯Ÿãããããã®é žã®å²åã
é«ããããã«ããªã°ãã³ã®åæã以äžã«ç€ºãã ããªãã¡ãããªã°ãã³ã¯ãçºé µå¹é€èäžã§æé·
ãã人çºçãªç现èãåäžããã³é«éã®é å¿åé¢
ãããè¿ãããšã«ãã€ãŠäžæ§ç·©è¡ç©ã§æœåºããåŸ
ãããæœåºç©ããçŽïŒãïŒã®ç¯å²ã®pKãæãã
èçœè³ªæåãåé¢ãããã®èçœè³ªæåããé«åå
éããªãã¡çŽ230000以äžã®ååéãæããç©è³ªã
åé¢ããããããçŽ10000ã®ååéãæããç©è³ª
ãåé¢ããããšã«ãã€ãŠã補ããããšãã§ããã 人çºçãªçްèå¹é€ã§è£œããããããªã°ãã³ã®é
ããã³ããªã°ãã³ã§ãã人çºç现èçºé µã§è£œãã
ããå šèçœè³ªã®å²åã¯ã倧ããªå¹é€å®¹åšäžã§ã®äºº
çºçãªç现èå¹é€èã®æé·ã«ãã€ãŠå¢å€§ããã ãã®æ¹æ³ã§è£œããããããªã°ãã³ã¯ãå®éçæ²
éçŽ ãã¹ãããã³ãªãŒããããã€ã²ã«æ¡æ£ãã¹ã
ã«ãããŠç¹æã®æäœãæããåäžç·ã®æ²éç©ã圢
æããé žæ§ãŸãã¯äžæ§PHãæããæ°Žæº¶æ¶²ããã³æ°Ž
ææº¶æ¶²ã«å¯æº¶ã§ãããã¢ã«ã«ãªæ§PHã§ã¯é£æº¶ã§ã
ããåå å 床èšã«ããåžåããŒã¯æ³¢é·ã280ïœÎŒ
ã§ãããçŽ10000ã®ååéãæããŠããããšãç¹
城ãšããŠããã ã¬ã³ã°ãã³ã®ç¹åŸŽã¯ããã«ãããã ã¢ã»ãã«ã»
ã«ããŒãºãšè€åããŠããã ã¢ã»ãã«ã»ã«ããŒãºâ
ã¬ã³ã°ãã³ã圢æããããšãå¯èœã§ãããåºä¹³å
ç©ã«æ³šå°ããéã«ç¹æã®æäœã§ããæã¬ã³ã°ãã³
ãçãããã®æã¬ã³ã°ãã³ãæ¬æ¥ã®äœçœ®ã§ã¬ã³ã°
ãã³åå ç©ã«ç¹ã«ä»çããããšã§ãããäŸãã°ã
ããçš®ã®æã¬ã³ã°ãã³ãæããªã°ãã³ã¯è©Šéšç®¡äž
ã«ãããŠè³è «ç现èã«æ¯æ§ã瀺ããã ã¢ã¹ããã·ãã³ãããªã°ãã³ããã³ãããã«é¡
䌌ããç©è³ªã®åŠãã¬ã³ã°ãã³ã¯ãçç©åŠç³»ã«å°å ¥
ãããŠãããç©è³ªãšããŠæçšã§ãã€ãŠãææãã¬
ã³ã°ãã³ã§è¢«èŠãããåŠãç°è³ªãªåå¿ãæžå°ãã
ããšãã§ãããçç©åŠç³»ã«ååŠé¢é£ç©è³ªãçãã
ããã«ã¬ã³ã°ãã³ã®ä»ã®å®äŸãå°å ¥ããããšãã§
ãããã¬ã³ã°ãã³ã¯ãŸããç¹å¥ãªçç©åŠç³»ã®æé·
ãæ é€çã«ä¿é²ããã®ã«äœ¿çšãããŠããããã¬ã³
ã°ãã³ã®æçšæ§ã¯ãŸããçç©åŠç³»ã«ãããé©å¿æ§
ãä¿é²ããããã«æ äœãæããã¬ã³ã°ãã³ã®è€å
ç©ããæãã¿ãŒã²ããïŒTargetïŒå€ã補é ã§ã
ãããšã«ããããããŠäŸãã°ãã®ã¬ã³ã°ãã³è€å
ç©ã¯ã¬ã³ã°ãã³èªäœã®ç©ççååŠçç¹æ§ãæããŠ
ãããæ äœã¯ãã¬ã³ã°ãã³ãšè€åç©ã圢æãããš
ãšãã«çç©åŠçã«å®è³ªçã«äžæŽ»æ§ã§ããç©è³ªãã
éžå®ãããã¹ãã§ããã ããªãããããŸãã¯èçœè³ªãšå®å®è€åç©ã圢æ
ãããããªãå ¬ç¥ç©è³ªãã¬ã³ã°ãã³ãšè€åããã®
ã«æçšã§ããããã®äžäŸãšããŠããã ã¢ã»ãã«â
ã»ã«ããŒãºã®åŠãã»ã«ããŒãºæããããçç©åŠç³»
ã«äžæŽ»æ§ã§ããããšã«å ããŠããã®æ äœã¯ãæ¬æ
现æžã§è¿°ã¹ãç®çã«æçšãªã¬ã³ã°ãã³ã®ç¹æã®ç©
ççååŠçæ§è³ªã倿Žããªããã®ã§ãªããã°ãªã
ãªãã ã¬ã³ã°ãã³ãšãã®æ äœãšããæãè€åç©ã¯ãæ¥
è§Šãããçç©åŠç³»ã®ååŠé¢é£ç©è³ªã補é ããåé¢
ãã確èªããã®ã«æçšã§ãããã¬ã³ã°ãã³âæ äœ
è€åç©ã¯ãŸããå°å ¥ãããçç©åŠç³»ã®ååŠé¢é£ç©
質åå ç©ã®è£œé ãä¿é²ããã®ã«æçšã§ããã ååŠé¢é£ç©è³ªã®ïŒé¡ã¯ãæã¬ã³ã°ãã³ããªãã¡
æã¢ã¹ããã·ãã³ããã³æããªã°ãã³ã§ãããã
ããã®ç©è³ªã¯çç©åŠç³»ã«ã¬ã³ã°ãã³ã泚å°ããŠè£œ
ããããšãã§ãããã¬ã³ã°ãã³ã®å ç«åŠçã«æå¹
ãªæçšéã¯ãåŸæ¥ã®æäœè£œé æè¡ã«åŸã€ãŠæäœå
å¿ãèªçºããããã«äœå çµç¹ãŸãã¯äœæ¶²ãšæ¥è§Šã
ããŠç®åºããããæã¬ã³ã°ãã³ã¯ãæã¬ã³ã°ãã³
ãæããè€å圢å€ãçç©åŠç³»ã«å°å ¥ããŠæãçç©
åŠç³»ã®ç¹ç°çްèãŸãã¯äœçœ®ã«èšºæå€ãæ é€å€ãã
ã³æ²»çå€ã®åŠãç©è³ªãæäžããã®ã«äœ¿çšããããš
ãã§ããããã®æã¬ã³ã°ãã³ã¯ãŸããçè²ãŸãã¯
æŸå°æ§åé¡è¡šç€ºãè «ç现èã«ã®ã¿çããããã«ã
ææããã³æŸå°æ§ç©è³ªã®åŠãåé¡è¡šç€ºç©è³ªãé å
ããæã¬ã³ã°ãã³ãçäœçµç¹ã«é©çšããããšã«ã
ã€ãŠãçäœçµç¹ã«è «ç现èãååšãããåŠãã蚺
æããã®ã«æçšã§ãããããã«æã¬ã³ã°ãã³ã䜿
çšããã®ã¯ãã¬ã³ã°ãã³ã®å ç«çã«æå¹ãªæçšé
ãåºä¹³åç©ãã®ä»ã®çç©åŠç³»ã«æ³šå°ããããšã«ã
ã€ãŠãä»ã®æçšãªååŠé¢é£ç©è³ªïŒäŸãã°TAGã
åŸè¿°ïŒã®åºä¹³åç©ããç£åºé«ãå¢å ããããã§ã
ãã åŸæ¥ãç³èçœè³ªè€åç©ã¯ãè³çµç¹ãã補é ã
ããæäœãè³çµç¹ã«åœ¢æãããŠãããã¿ã€ãµãã¹
ïŒTayâSachsïŒã®ç çãªè³ãã補ãããã10Bç³
èçœè³ªãšããŠç¥ãããåé¢ç©è³ªã¯ãã©ããããã
ã³è£œããããæäœã«æ³šå°ãããŠããããããã®ã¿
ã€ãµãã¹æäœã¯ãè «çãæããè³ã®å ç«è¢å æ§ç
ç©¶ã«äœ¿çšãããŠãããè «çã°ãªã¢ã§ã¯ãªãåå¿ç
ãªæ®éã®éè «çã°ãªã¢ã®ã¿ããããã®æäœã«ãã€
ãŠçè²ãããŠããã ãããšå¯Ÿç §çã«ãã¢ã¹ããã·ãã³ãè «ççµç¹ã
ã補ããããæãã¢ã¹ããã·ãã³ã«å¯Ÿããæäœ
ïŒæã¢ã¹ããã·ãã³ïŒã補ããããè³ã®å ç«è¢å
æ§ç ç©¶ã«äœ¿çšãããæ®éã®ïŒéè «çïŒã°ãªã¢ã§ã¯
ãªãè «çã°ãªã¢ã®ã¿ãæã¢ã¹ããã·ãã³ã«ãã€ãŠ
çè²ãããŠããããããã€ãŠãåå çµç¹ãæäœã®
æ§è³ªããã³çè²ããã现èç¹æã®ã¿ã€ãã«ãã€
ãŠãæã¿ã€ãµãã¹æäœããã³æã¢ã¹ããã·ãã³ã¯
ç°ãªãç©è³ªã§ããã®ã¯æããã§ããã ååŠé¢é£ç©è³ªã®ä»ã®é¡ã¯ããããã®ååŠé¢é£ç©
質ãšè€åããã¿ãŒã²ããå€ã§ãããäŸãã°ãã¿ãŒ
ã²ããïŒããã ã¢ã»ãã«âã»ã«ããŒãºã®åŠãæ äœ
ãšè€åããã¢ã¹ããã·ãã³ç©è³ªïŒã¯æã¢ã¹ããã·
ãã³ãšã®æ¥è§Šã«äœ¿çšãããããã®ã¿ã€ãã®ååç©
ã¯ãè€åãããŠãçç©åŠç³»ã®ç¹ç°çްèãŸãã¯äœçœ®
ã«èšºæå€ãæ é€å€ããã³æ²»çå€ãæäžããããã«
䜿çšããããšãã§ããããããã®ååç©ã¯ãŸãæŽ
æµåŠçã®ããã«ã䜿çšã§ãããäŸãã°ãæã¢ã¹ã
ãã·ãã³ã¯ãããã ã¢ã»ãã«ã»ã«ããŒãºâã¢ã¹ã
ãã·ãã³âæã¢ã¹ããã·ãã³ãé žãŸãã¯ãããã€
ããŒãŒé µçŽ ã§å æ°Žåè§£ããŠè£œããããšãã§ããã
ã¿ãŒã²ããå€ã¯ãŸããã¿ãŒã²ããã®å ç«åŠçã«æ
å¹ãªæçšéãäœå çµç¹ãŸãã¯äœæ¶²ã«æ¥è§Šããããª
ã©ã«ãã€ãŠãçç©åŠç³»ã®TAGç©è³ªïŒåŸè¿°ïŒã®é
ãå¢å ããã®ã«åœ¹ç«ã€ã ä»å çãªååŠé¢é£ç©è³ªã¯TAGå€ïŒã¿ãŒã²ãã
ã«ä»çããã°ãããªã³ïŒã§ãããTAGç©è³ªã¯ã
TAGãè€ååè§£ããããã®æéãå€åãããã
ã«ã¿ãŒã²ããå€ãäœæ¶²ãšæ¥è§ŠãããŠåŸãããã äºã€ã®æçšãªå®æœæ æ§ã¯ïŒ³âTAGåã³ïŒŠâ
TAGã§ããã âTAGïŒSLOWâTargetâAttachingâ
GlodulinïŒã®è£œé æ¹æ³ã¯ãè¡æž ãã®ä»ã®äœæ¶²ãæš
çïŒå³ã¡ãããã¢ã¢ã»ãã«ã»ã«ããŒãºâããªã°ã
ã³ïŒãšçŽïŒæéåã¯ãã以äžãäœæž©äŸãã°çŽïŒâ
ã§åå¿ãããâTAGãçæç©ããéè£ããã
ãšãäŸãã°çŽ37âã®æž©åºŠã§çŽïŒæéçšéé žã§éè£
ããããšããæãããã®æ¹æ³ã«åŸã€ãŠè£œé ããã
補åâTAGã¯æ°Žæ§ç·©è¡æ¶²ã«å¯æº¶ã§ãããã¢ãŠ
ãã¿ãŒãããŒïŒOuchterlonyïŒã²ã«æ¡æ£è©Šéšã«ã
ããŠãã®çžåœã¬ã³ã°ãã³ãšã®åç³»æ²æŸ±ç©ãçæ
ããã»ããã¢ã³èã§éæäžèœã§ããã25000åå
é以äžã®ååãä¿æããããªãã¢âãã€ã«ã¿ãŒã§
çä¿ããããã¯ãã»ã°ãããªã³åãçŽ50000åã³
ãã®åæ°ã®èå±€ã²ã«ã»ã¯ãããã°ã©ãã€ãŒã«ãã€
ãŠå®ãããããããªç°ã€ãç¶æ ã®ååºãããåå
éãæãããããŠ280ïœÎŒã®åå å 床èšåžåããŒ
ã¯æ³¢ãæããããšãç¹åŸŽãšããã âTAGïŒFastâTargetâAttachingâ
GlobulinïŒã補é ããæ¹æ³ã¯ãè¡æž ãã®ä»ã®äœæ¶²
ãæšçïŒå³ã¡ãããã ã¢ã»ãã«ã»ã«ããŒãºâããª
ã°ãã³ïŒãšçŽ10åéäœæž©äŸãã°ïŒâã§åå¿ããã
âTAGãçæç©ããéè£ããããšãäŸãã°çŽ
37âã®æž©åºŠã§çŽïŒæéçšéé žã§éè£ããããšãã
æãããã®æ¹æ³ã«åŸã€ãŠè£œé ããã補åâ
TAGã¯æ°Žæ§ç·©è¡æ¶²ã«å¯æº¶ã§ãããã¢ãŠãã¿ãŒã
ããŒïŒOuchterlonyïŒã²ã«æ¡æ£è©Šéšã«ãããŠãã®
çžåœã¬ã³ã°ãã³ãšã®åç³»æ²æŸ±ç©ãçæããã»ãã
ã¢ã³èã§éæäžèœã§ããã25000ååé以äžã®å
åãä¿æããããªãã¢ã»ãã€ã«ã¿ãŒã§çä¿ããã
ãã¯ãã»ã°ãããªã³åããçŽ50000åã³ãã®åæ°
ã®èå±€ã²ã«ã»ã¯ãããã°ã©ãã€ãŒã«ãã€ãŠå®ãã
ãããããªç°ã€ãç¶æ ã®ååºãããååéãæ
ãããããŠ280ïœÎŒã®åå å 床èšåžåããŒã¯æ³¢é·
ãæããããšãç¹åŸŽãšããã TAG補åã¯æ¢ç¥éã®åºä¹³åç©ã®è¡æž ãã®ä»ã®
äœæ¶²ã«ãã€ãŠäœãããâTAGåã³ïŒŠâTAGã®
æ¿åºŠã決å®ããŠãã®æ¿åºŠãšãçã瀺ããã®ãšããŠ
å®ããããå€ãšãçžé¢ãããããšã«ãã€ãŠãçã
ãåºä¹³åç©ã®çè «çãæ¢ç¥ããã®ã«æçšã§ããã
TAG補åã¯ãŸãçµç¹éšäœã®è «ç现èã®ååšã蚺
æããã®ã«æçšã§ããããããã¯ææãšãæŸå°æ§
ç©è³ªã®ãããªæšèç©è³ªãšæ¥åããTAGãåœè©²éš
äœã«é©çšããããšããæããããã«ãã€ãŠè «ç现
èã«ã®ã¿çè²åã¯æŸå°æ§æšèãçãããã®ã§ã
ããæŽã«TAG補åã¯è «ç现èã«å¯ŸããŠçŽ°èæ¯æ§
ãããããšã倿ãããTAG補åã¯ãŸãã蚺æã
æ é€ãåã³æœççšã®å€ãTAG補åãšé¯ç¶ã«ãã
ãã®ãå°å ¥ããããšã«ãã€ãŠããããã®å€ãç¹å®
ã®çްèåã¯éšäœã«å±ãããã®ã«æçšã§ããã 现èãå®å šã«ç¹å®ã®å Žæãå ããããã«ãªã
ãšãæ€ç©åã¯åç©ã«ãããæ£åžžã®çްèåå²ã¯å¶é
ããããåã¯ææ¢ãããã(a)æ£åžžã®çްèããèªå·±
ã®å ãåŸãå Žæãåããããšããèªèãããæ©æ§
åã³(b)ãã®èªèæ©æ§ã®äœçšãä»åºŠã¯çްèã®åå²ã
ææ¢ããæ©æ§ã¯ãå ±ã«ç¥ãããŠãããæ£åžžã®èªè
åã³æç¥ãçãããšãå é§ç©è³ªã®æ¿åºŠãå¢å€§ãã
ç²ååã³çްèã®èªèåã³æç¥ã«é¢ä¿ãããããªäž
矀ã®ååç©ã§ãã€ãŠçްèãäºãã«é£çµãããã®ã
補é ãããããã«ãªã€ãããããã®ååç©ã¯ã¬ã³
ã°ãã³ãºãšåŒã°ãããéåžžã®ç现èãããããã®
ååç©ã補é ããããšè©Šã¿ããšããããããã¯ã
ã®ãããªãã®ãšããŠã¯ãªãããšããããããŸãã
ç现èã(a)ãã®æ£åžžãªäœç©ãå ããããšãèªèã
ãèœåãåã³ïŒåã¯(b)ãã®æ£åžžãªäœç©ãå ããé
ã«åå²ãæ¢ãããšããèœåãã倱ããšåæã«ãã®
ååæ§é ã«å€åãçããããšãããã€ãã æ°èŠãªååç©ãªãã³ã«ãã®ãããªååç©ã補é
ããæ¹æ³ãçºèŠãããããããã®æ°èŠååç©ã¯ã¬
ã³ã°ãã³ãºãšåä»ãããããã¬ã³ã°ãã³ãºã¯ã现
èåå²ãèªèãæ¢ããããšãã§ããªããšããç¹ã§
ç现èã®åœ¢æ çç¹æ§ãæš¡å£ããç©çååŠçç¹æ§ã
æããæ°èŠååç©ã§ãããã¬ã³ã°ãã³ã®äœ¿çšã¯ç
ã®äœç𿩿§ãèŠãããã以äžã«ãããããã®ã§ã
ããäœæ ãªãã°ããã«ãã€ãŠçã®èšºæåã³æ²»çãª
ãã³ã«ãã®äºé²ã«åœ¹ç«ã€çŽæ¥è£œåãªãã³ã«æ¹æ³ã
æäŸãããããã§ããã ããªã°ãã³ãºïŒMALIGNINSïŒã補é ããã®
ã«äººå·¥çã«å¹é€ãã现èãçšããããšãã§ããæ¹
æ³ãçºèŠããããããã«é瀺ããæ¹æ³ã®å©ç¹ã®äž
ã€ã¯ãããªã°ãã³ãºïŒMALIGNINSïŒåã³ãã
ããäœãæ°è£œåãäºå®äžç¡å¶éã®éã§å¹ççã«è£œ
é ããããšãã§ãããšããããšã§ããã æ¬çºæã¯çç ç©¶ã®åéã«åªããã®ã§ããããŸã
ãã¹ãŠã®çé·åã³æ°é³ä»£è¬ã«åœ±é¿ãäžããããšæ
ãäžåã®çç©åŠç³»ã«çŽã¡ã«é©çšã§ãããã®ã§ã
ãããããã«ã人工çå¹é€ããé©åœãªçްèåã®ç¹
å®ã®ååç©ã補é ããããšã«ããããŸããããã®
ç©è³ªãã補åãæŽã«è£œé ããããšã«ãããå ãæ
åã«çç©ç³»ã«ãããçµç¹ã现èãçŽ°èæ©èœè³ªã次
æ©èœè³ªååãããã¯ååéåäœã«ç¹å®ã®åœ±é¿ãå
ãŒãããšãã§ããããããããŠãçºè²æã®éèŠæ
æã«ãããç¹å®ã®æ é€å¹åãç¹å®ã®èšºæäžã®ãäº
é²äžã®ãåã³æ²»çäžã®æ¹æ³ãåã³äººå·¥ç黿°ã·ã¹
ãã ã®æ§æïŒçµç¹åã¯åšç®¡ç§»æ€ã«ãããåŠãïŒã
ãã¹ãŠæåã«åœ±é¿ãåããããšãã§ãããããã
ã®äººå·¥ç黿°ç³»ã¯ä»ããããã«é£ããããšããªã
éåžžã®çµç¹åã¯çµæã®ç¹å®ã®ã¬ã³ã°ãã³
ïŒRECOGNINïŒãããªã°ãã³ïŒMALIGNINïŒå
ã¯ãããã®ã±ã¢ã¬ã·ããã«ã«ãº
ïŒCHEMORECIPROCALSïŒã®ç¹æ§ãæã€ããã«
ããããšãã§ããã®ã§ããç°ç©ããšããŠãèªèã
ãããããããæçµ¶ãå«ãç°ç©ã«å¯Ÿããåå¿ãé¿
ãããã®ã§ããã æ¬çºæã®ä»ã®æ æ§ã¯ãç¹å®ã®é è³è£œåïŒã¢ã¹ã
ãã·ãã³ïŒã«å¯Ÿãã䟡å€ã®é«ãç¹å®ã®æäœç¶ã®è£œ
åïŒã¢ã³ãã»ã¢ã¹ããã·ãã³ïŒã補é ããããšã§
ããããã®æäœç¶è£œåãããããçš®ã®ç¥çµç³»ã®ç¹
å®ã®ç¹ã«ç¹å®ã«é¯ç¶ãšãªãããã«ããããŠããã«
察ããç¹å®ã®äŒéæ äœãšããŠçšããããšãã§ãã
ããšã§ãããããªã°ãã³ãºïŒMALIGNINSïŒå
ã³ã¢ã¹ããã·ãã³ïŒASTROCYTINïŒã¯ã¬ã³ã°
ãã³ãºïŒRECOGNINSïŒã§ããã æ¬çºæã®æŽã«ä»ã®æ æ§ã¯ãäºã€ã®æ°è£œåã®çç©
åŠçæµäœããTARGETâATTACHINGâ
GLOBULINSïŒTAGïŒã補é ããããšã§ããã
ããã¯äºã€ã®åå¿å³ã¡ç¬¬äžã¯çç©åŠçæµäœãããª
ã°ãã³ãºïŒMALIGNINSïŒãæš¡å£ããç©çååŠ
ç圢æ ãå«ãåæé¯äœãšåå¿ããããã®ã§
TARGETãšåŒã°ãã第äºã¯ç¹å®ã®TAGãé¯äœ
ããéè£ããããã®ã§ããã®ææ©äœã«è «çããã
ãã©ããçããææ©äœã®çç©åŠçæµäœããã®å®é
ç衚瀺ãåŸãŠãå³ã¡è «çã®èšºæè©Šéšããããã®ã
ãã«äœãããTAGã®å¯žæ³ã«ãã€ãŠãã®ããã«åœ
åããããã®ã§ãããTAG補ååã³ANTIâ
MALIGNINã¯ç©çååŠçã«MALIGMINãè£ãª
ããã®ã§ããããããããã¯
CHEMORECIPROCALSãšåœåãããã æŽã«ãè¡æž ãšäœ¿çšããç¹å®ã®TARGET詊è¬ãš
ã®åå¿ã«èš±ãããæéã«ãã€ãŠããŸãé¯åããã
補åã®éè£ã«èš±ãããæéã«ãã€ãŠãäºã€ã®å®é
çåã³å®æ§çã«æãã«åºå¥ãããTAG補åã補
é ããããšãã§ããããšãçºèŠãããã è³è «çã®ãããåã³çš®ã ã®ä»ã®å»åŠçæ éã®ã
ãããªãã³ã«ãªããæãããªçŸç ã®åŸŽåã®ãªãå€
æ°ã®ç°ã€ã人ã ãã補é ããããšãã§ããããã
ã®è£œåã®éãæ€æ»ããåŸãäžå®ã®åºäººã«ã€ããŠè£œ
é ããããšãã§ãããããã®äºã€ã®æ°èŠè£œåã®é
ã¯ããã®å人ã«è³è «çããã€ããã©ããã瀺ãã
ã®ã§ããããšãæãããšãªã€ããããããè³è «ç
ã®æ°èŠè¡æž 蚺æè©Šéšæ³ãçºèŠãããã®ã§ããã ãããã®æ°èŠè£œåã®è¡æž ãã®ä»ã®çç©åŠçæµæ¶²
ããè³ãã®ä»ã®è «çã蚺æããçšéã®ã»ãã®å¹çš
ã¯ãTAGåã³æRECOGNINååç©ã¯è³è «çã®
å€ç§æè¡ã§åé€ãããè³è «çåã³ãã®åšå²ã®çµç¹
ã®çµç¹åŠçéšäœã«ãããŠåªå çã«ç¥çµè è «ç现è
ã«ã€ããšãã蚌æ ã§èª¬æãããããã®è «ç现èã®
TAGåã³æRECOGNINSã«ããåªå çæšèã¯æš
æºå ç«è¢å æè¡ã«ãã€ãŠèšŒæãããããã®ãã
ã«ãæ°èŠæ¹æ³ã¯ãŸããçµç¹åŠçæ€æ»ã«ãã€ãŠæ°èŠ
ãªç¢ºåºŠããã€ãŠãè «ç现èãé€å»ããçµç¹ã®æ¥µç«¯
éšãŸã§æµžéããŠãããã©ãããæ±ºå®ããã®ã«çšã
ãããšãã§ããè «çãè³ãã®ä»ã®åšå®ã«ãŸã æ®ã€
ãŠããå¯èœæ§åã¯è «ç现èãé€å»ããçµç¹ã®åšèŸº
ãããªããªã€ãŠããå¯èœæ§ã瀺ããè «çã®ãã¹ãŠ
ãè³åã¯ä»ã®åšå®ããåé€ãããå¯èœæ§ã瀺ãã
ãšãã§ãããæŽã«ãèšè¿°ããããã«è£œé ãã
TAGåã³æMALIGNINã¯è©Šéšç®¡å ã§çµç¹å¹é€
äœã«çæããè è «è³è «ç现èã«å¯ŸããŠçŽ°èæ¯æ§ã
ããããšãããã€ããããã§ã¯çµç¹å¹é€äœã«çæ
ããããä»ã®åªäœã§ã®è «ç现èã«å¯Ÿãããã®é«èŠª
åæ§ã¯ãæ°è£œåTAGã®ç¹å®çµåæœåšåãæŽã«èšŒ
æãããã®ã§ãããåæè£œåTARGETã«é¢ããŠ
åã³çµç¹åŠçéšäœã«ãããè «ç现èã«é¢ããŠ
TAGã®æ§è³ªã衚ããããã«TARGETâ
ATTACHINGâGOBULINSïŒTAGïŒã®åç§°ã®
æ¡çšã説æãããã®ã§ãããæŽã«ãè «ç现èã«å¯Ÿ
ããTAGåã³æRECOGNINSã®çŽ°èæ¯æ§ã¯è «ç
ã«ããã€ãçãã®ããæ£è ã®è¡æž ã®æŽã«æ°ãã蚺
æè©Šéšæ³ãæäŸããããã®ããã«ãäŸãã°ããã
ãã®æ£è ã®è¡æž åã¯ä»ã®äœæ¶²ãTARGETãšåå¿
ãããŠTAGã補é ãã補åTAGã¯çµç¹å¹é€äœäž
ã§è «ç现èã®çé·ãçŽ°èæ¯æ§æ€æ»ãããäžå®ã®å
人ã®è¡æž ãã補é ããããšãã§ããTAGã«ãã€
ãŠçŸããããTAGã®æ¿åºŠåã³çŽ°èæ¯æ§åºŠã¯åã«
蚺æäžã®å©ç¹ãããã°ããã§ãªããç¹å®ã®æ£è ã«
ã€ããŠã®æè¡ååã³æè¡åŸã®äžèª¿ã®çµéãæ¢çŽ¢ã
ãäžã«ã䟡å€ã®ãããã®ã§ãããæŸå°æ§åã³æè²
æ¢ç¥ç©ãTAGãšçµåããã°ãè «çã®èšºæåã³ã
ã®æ£ç¢ºãªéšäœã®å€å®ãããå Žåçäœã«æçšã§ãã
æ°èŠãªTAG補åãäœãããšãã§ããããããã«
ããŠãåèå åã¯éèå ã®ããããã«é©åœã«æšè
ããTAGãè³è髿¶²å ã«ãåã¯çŽæ¥è³çµç¹åã¯
ãã®çª©ã«æ³šå°ããã°ãæŸå°æ§ææ®µã«ãããåã¯çµ
åããææã®èŠèŠåã«ãããè³è «çã®ååšã説æ
ããããšãã§ããããšããã®ã¯TAGãç¹å¥ã«ä»
çããã®ã¯è «ç现èã«ã®ã¿ã§ããããã§ãããæŽ
ã«ããã®æ¹æ³ã¯è³è «çã®éšäœãæ£ç¢ºã«èŠèŠåãã
ããšãã§ãããããã¯è³è «çãæšèããããå ã®
è¡æ¶²ã§äœã€ãæASTROCYTINãçšãããã®ç
äœå èšºææ¹æ³ã®æ¹è¯ãšèŠãªãããšãã§ããããªã
ãªãã°äººéã®è¡æž ããäœã€ãTAGãçšããã°ç°
èçœåå¿ã®å¯èœæ§ãé¿ããããããã§ããã
TAGåã³æRECOGNINSã¯è©Šéšç®¡å åã³çäœå
ã®åæ¹ã§ã®è «ç现èãå«ãASTROCYTINåé§
ç©è³ªã«åªå çã«ä»çããããšãã§ããååŠçç¹æ®
æ§ãæã€ã®ã§ãäŸãã°æŸå°æ§ã®ããããã³è£ç²å€
åã¯ä»ã®æ¯æ§ã®ããç©ççåã¯ååŠçå€ãšçµåã
ããŠããããã®æ¯æ§ã®ããç©è³ªãè «ç现èã«é£æ¥
ããæ£åžžçŽ°èãšæ¯èŒããŠè «ç现èã«ä»çããç¹æ®
æ§ã«ãã€ãŠåªå çã«å±åšåããããšãã§ãããã
ã«ã蚺æåŠçã«ãšåããæœççã«ããããã®è£œå
ãçšããããšãã§ããããã®éžææ§ã¯ãè «çã®å¹
æçãªååŠçåã¯ç©ççæœçãéæããããã®æ±º
å®çãªãå°ããšãäžã€ã®æ±ºå®çãªèŠçŽ ãšããŠããŸ
ããããŸã§éæãããªãã€ãèŠçŽ ãšããŠãæ®éç
ã«èªããããããããã«ãTAGã¯åªå çã«è «ç
现èã«ä»çããç¹ã§å¹æã®ããããšã蚌æããã
ã®ã§ããããããã®çç±ã§æ°èŠãªæ²»ç補åãšããŠ
ã®æææ§ãæã€ã¹ããã®ã§ããã æªæ§ã®è «çãæã€ãæ£è ã®è¡æž ã«ãããŠã¯ãäž
èšã®å®æœäŸã«èŠãããããã«ãFASTâTAGïŒïŒŠ
âTAGïŒãšåºå¥ãããäžã€ã®åã®TAGãå³ã¡
SLOWâTAGïŒïŒ³âTAGïŒããããããªè «çã®
ãªãæ£è ã«ãããããããäžå®éã®è¡æž ããæ¯èŒ
çå€éã«è£œé ããããšãã§ããããã®ããšã¯ã
TAGã®èªç¶ã«çºçããåé§ç©è³ªïŒïŒ°âTAGïŒã®
ããããäžã€ã®æ¿åºŠãå¢å€§ãããåã¯ïŒŠâTAG
ãããâTAGãæ¯èŒç詊éšç®¡å ã§è¯ãåºæ¥ã
ãããªä»ã®èŠå ãããããšã瀺åãããã®ã§ã
ãã å®éã®åæè£œåTARGETåã³TAGã®ãã®å
é§ç©è³ªã«å¯ŸããããããŠãäºããããã°ãçäœå
ã«ååšããããšãããåŸããããã«å¯Ÿããä»®å®ã
ããã蚌æãããŠãªã现èãæåãåã³åŸªç°ãã
ãæäœãã®äœçšã«å¯Ÿãããäœçšã®ããåŸãé¢ä¿ã¯ã
ãŸã è§£æãããŠããªãããã®ããã«äŸãã°ãæäœ
ç¶ã®æ§åŒã§ãâTAGåã³ïŒ³âTAGã¯ã¢ãŠãã¿
ãŒãããŒã»ã²ã«æ¡æ£ã«ãããŠASTROCYTINãš
åäžã§å¥ã ã®ç³»çµ±ã®åå¿ãçæãããããã§ãå
ã«TARGETãæ³šå°ããã°ãTARGETãšåå¿ã
ãåŸãå ã®è¡æž ããäœãTAG補åã®åçãå¢ã
ããããšã«ãªãã埪ç°ããæäœã«äŒŒãåé§ç©è³ªã
åå²ããªã现èã«é ãããŠãã现èæåã«å¯ŸããŠ
æ£åžžãªæ°ŽæºãããåŸããšããçºèŠã¯ãäžå¯Ÿã®ã
ã®ãããåŸãäœçšã«ã€ããŠã®åé¡ãæèµ·ããã
ããã§ã现èã®å¢æ®åã³çްèã®æ»æ» ã®å¶åŸ¡ã«äœçš
ããTAGåé§ç©è³ªïŒïŒ°âTAGïŒåã³TARGET
ç¶ç©è³ªãçäœå ã«ååšããããšãæèµ·ããããã
ãã«ãäŸãã°ãéåžžã¯çŽæ¥ã¯è¡æž èçœã«æããã
ããšã®ãªãçŽ°èæ§æç©ã®é²åºã现èåå²äžã«èµ·ã
ããšãããããã®çŽ°èæ§æç©ã®é²åºã®çµæãæ§æ
ç©ã¯TARGETç¶ç©è³ªã«å€æãããããã«ãªãã
ããã«è¡æž ããâTAGç¶ååã®ä»çãçãã
ããã现èåå²ãåºæãããåã¯ææ¢ããããšã
èããããããããã¯ãå·å®³ãåããåã¯äœçšäž
è¯ã®äžåå²çްèã¯ïŒ°âTAGç¶ååã®ä»å ãå埩
å¯èœã§ãããããªTARGETç¶ç©è³ªãé²åºããã
ããšãããããããããã现èç¶æ ã®äžã§ã¯ïŒ°â
TAGç¶ååã®ä»å ã¯çްèã®ç Žå£ãæãããšãã
ãïŒäŸãã°ããã«èšèŒããåæã§è£œé ããANTI
âGLIOMAâTAGã¯çµç¹å¹é€äœäžã§çé·ããè
è «ç现èã«èããçŽ°èæ¯æ§ãããïŒããããã«ã
ããã¯ã现èåå²ã®å¶åŸ¡ã«ã€ããŠããŸãææ©äœã®
çæ¶¯ãéããŠäœå ã®åã ã®çްèã®ä¿®åŸ©åã¯é€å»ã«
ã€ããŠã®æ£åžžãªæ©æ§ã®æš¡ç¯ã瀺ããã®ãšããã
ããè³å è è «ã®å Žåã®ããã«æ¥éã«åå²ããç现
èã«çããããã«ãçŽ°èæ§æç©ã®é²åºãç°åžžã«å¢
倧ããŠç°åžžã«å€§éã®çްèTARGETç¶ç©è³ªã圢æ
ãããå Žåã¯ãäžã€ã®åã®è¡æž âTAGã®æ¿åºŠ
ãä»ã®ãã®ã«æ¯ã¹ãŠå¢å€§ããããšãèµ·ãããšãã
ãã åèšç©è³ªã®å®éã®äœçšãã©ã®ãããªãã®ã§ãã
ããšãããã«èšè¿°ããæ¹æ³ã«ãã€ãŠæªæ§ã®è «çã®
ããæ£è ã®è¡æž ãã詊éšç®¡å ã§è£œé ããããšãã§
ããTAGã®äž»èŠãªäžã€ã®åãSLOWâTAGïŒïŒ³
âTAGïŒã®çžåœéã®å¢å ã¯ãäžèšã®å®æœäŸã«èš
èŒããè¡æž 蚺æè©Šéšã®åºç€ãšãªããã®ã§ããã 宿œäŸ ïŒ ç²ã¢ã¹ããã·ãã³å é§äœå«æåç»ã®çæïŒâ 人éã®è³è è «è «è¹çµç¹ãåãåãã衚é¢ã®è¡ç®¡
ãšæ®éã®è³çµç¹ãšãå¯èœãªéãåé¢ããããåé¢
ããè «è¹çµç¹ã®ä»£è¡šçãªé11ïœããšããããã
1.5ïœã®éšåïŒåããã³1.0ïœã®éšåïŒåã«åã
ããã€ãã§åéšåãæ¬¡ã®ããã«åŠçããã åéšåãäžæ§ç·©è¡æº¶æ¶²äžãè¶ é³æ³¢ãŸãã¯ä»ã®æ©
æ¢°çææ®µã«ããå質åãããããšãã°ãåéšåã
ã¯ãŒãã³ã°æ··åæ©äžã0.005Mçé žç·©è¡æ¶²ïŒPHïŒïŒ
ã®100c.c.ïŒïœçµç¹ã«å質åãããèçœã®å€æ§ãé²
ãããã«ãäžèšå質ååŠçã¯å·æ¡ä»¶äžã«è¡ãã§ã
ã§ãããããšãã°ãè©²æ··åæ©ãïŒãïŒâã®å·å®€ã§
ãããããå·ãããçŽïŒåéã§æäœãã¹ãã§ã
ãã ã€ãã§ãåçè³ªãæž æŸã«ããããã«ãããšãã°
å·åããè¶ é å¿åé¢ã§80000åéåã30åéäœçš
ãããŠé å¿åé¢ãããã坿º¶æ§äžæŸæ¶²ãåŸæåé¢
ããå·äžã«ä¿ã€ãäžæº¶æ§æ®æž£ã¯ãããã«äžæ§ç·©è¡
æ¶²100mlãçšããŠåã³å質åããäžèšãšåæ§ã«é
å¿åé¢åŠçããåŸãããïŒçªç®ã®å¯æº¶æ§æœåºç©ãš
äžèšïŒçªç®ã®ãããšåãããäžæŸæ¶²äžãèçœã50
ãã€ã¯ãã°ã©ã ïŒml溶液以äžãšãªããŸã§å質åãš
é å¿åé¢ãããããããšããåçã¯éåžžã«è¯å¥œã§
ããããã®ããšã¯ãã»ãšãã©ã®çµç¹ã®å ŽåãïŒå
ã®æœåºã§éæãããã ãã®ããã«ããŠåŸã溶液ãåããã€ã¥ãéæã§
ååã«èžçºãããããšã«ããæ¿çž®ãããå·äžã
0.006Mçé žç·©è¡æ¶²ã®éæã«ãã15mlã®æº¶æ¶²ãåŸ
ãããã®æº¶æ¶²ã®éãèšé²ããäžéšãå šèçœã®åæ
ã«çšããæ®äœãåããŠpK1ãïŒã®èçœè³ªåç»ãåŸ
ãã以äžã«è¿°ã¹ãã¯ãããã°ã©ãã€ã¯å¥œãŸããå
奿³ã§ããã æº¶æ¶²ããå·å®€ïŒïŒâïŒäžã0.005Mçé žãããª
ãŠã ç·©è¡æ¶²ã§å¹³è¡¡ãããDEAEã»ã«ããŒã¹
ïŒCellexâïŒã«ã©ã 2.5Ã11.0cmäžã§åå¥ããã
æº¶åºæº¶åªã¯ä»¥äžã®æº¶åªïŒæº¶æ¶²ïŒãçšããããšã«ã
ãæ®µéçã«å€ããïŒæº¶æ¶²(1)NaH2PO44.04ïœãã
ã³Na2HPO46.50ïœãèžæºæ°Ž15000mlã«æº¶è§£ãã
ïŒ0.005ã¢ã«ãPHïŒïŒïŒæº¶æ¶²(2)NaH2PO48.57ïœãèž
æºæ°Ž2480mlã«æº¶è§£ããïŒæº¶æ¶²(3)NaH2PO417.1ïœ
ãèžæºæ°Ž2480mlã«æº¶è§£ããïŒ0.05ã¢ã«ãPH4.7ïŒïŒ
溶液(4)NaH2PO459.65ïœãèžæºæ°Ž2470mlã«æº¶è§£ã
ãïŒ0.175ã¢ã«ïŒïŒæº¶æ¶²(5)NaH2PO4101.6ïœãèžæº
æ°Ž2455mlã«æº¶è§£ããïŒ0.3ã¢ã«ãPH4.3ïŒïŒæº¶æ¶²(6)
NaH2PO4340.1ïœãèžæºæ°Ž2465mlã«æº¶è§£ãã
ïŒ1.0ã¢ã«ãPH4.1ïŒïŒæº¶æ¶²(7)80ïŒ çé žïŒH3PO4ïŒ
283.64ïœãèžæºæ°Ž2460mlã«å ããïŒ1.0ã¢ã«ãPH
1.0ïŒ ç¥çµçµç¹æœåºç©ïŒã10mlãå ããããããã«ã©
ã ã«éããã€ãã§æº¶æ¶²(1)ãäžã«å ããæº¶æ¶²(1)300
mlãåãã貯çåšãåä»ããŠéåã§ã«ã©ã ã«èœäž
ããããèªåçãªåç»åéè£ çœ®ãçšããŠæº¶åºæ¶²ïŒ
mlãéãããã€ãã§ãæ¬¡ã®æº¶åºç®¡çªå·ã§ãæº¶åºæº¶
æ¶²ãå€ããŠçšãããæº¶æ¶²(2)ïŒç®¡ïŒïŒã§ãã«ã©ã äž
溶液ãã¬ãžã³ã®é éšã«éã³ãã€ãã§æº¶æ¶²(2)50mlã
äžããå ã貯æºåšãåä»ããïŒç®¡ïŒïŒã§ã溶液ã
ã«ã©ã äžãã¬ãžã³ã®é éšã«éã³ãã€ããŠã溶液(3)
75mlãäžããå ãã貯çåšãåä»ããïŒæº¶æ¶²(4)ïŒ
管ïŒïŒïŒã§ã溶液ãã«ã©ã äžãã¬ãžã³ã®é éšã«é
ã³ãã€ãã§æº¶æ¶²(4)150mlãäžããå ãã貯çåšã
åä»ããïŒæº¶æ¶²(5)ïŒç®¡ïŒïŒïŒã§ã溶液ããã«ã©ã
äžãã¬ãžã³ã®é éšã«ãã€ãŠãããã€ãã§æº¶æ¶²(5)ã
äžããå ãã貯çåšãåä»ããïŒæº¶æ¶²(6)ïŒç®¡ïŒïŒ
ïŒã§ã溶液ãã«ã©ã äžãã¬ãžã³ã®é éšã«ãã€ãŠã
ããã€ãã§æº¶æ¶²(7)175mlãäžããå ãã貯çåšã
åä»ããïŒç®¡ïŒïŒïŒã«å°ããŸã§æº¶åºãç¶ããŠæº¶åº
ãå®äºãããçµç¹æœåºç©ã®å容ããšã«æ°ãã«è£œã
ãã¬ãžã³ã䜿çšãããå管ãèçœã®å®éåæã«ä»
ãã管ïŒïŒïŒãïŒïŒïŒå ã®æº¶åºæ¶²ãåããããã¯
ç²çæç©ãå«ã¿ãããããã¢ã¹ããã·ãã³ãåŸã
ããã ãã®éå»ã«ãããŠãã©ã¯ã·ãšã³10BãšåŒã°ãã
ç²çæç©ã«ã€ããŠã®ããŒã¿ã¯å ¬ã«ãããŠããã
ããããã€ã³ã»ã¡ã¿ããªãºã ã»ãªãã»ã¶ã»ããŒã
ã¹ã»ã·ã¹ãã pp555ã69ïŒPleum Pressã1970
幎ïŒïŒãžã€ãŒãã«ã»ãªãã»ãã€ããµãŒãžã€ãªãŒã
Vol.33ãpp281ã286ïŒ1970å¹ŽïŒæïŒåç §ïŒãããã
ã§ã¢ã¹ããã·ãã³ãšåŒã°ããç¹å®ã®çæç©ãåç»
ïŒïŒïŒ¢ããåé¢ããããšãéæãããç²åç»ïŒïŒ
ã¯ãã¯ããã®æ°é®®ãªç¥çµç³»çµç¹ïŒïœã«ã€ã0.1
ã10mgã®çæç©ãšããŠè£œãããããããã¯ã¢ã¹ã
ãã·ãã³å é§äœã®ä»ã«ã倿°ã®ãããœã·ã¹ãããª
ãã¡ã°ã«ã³ãŒã¹ãã¬ã©ã¯ããŒã¹ããã³ããŒã¹ïŒã°
ã«ã³ãŒã¹ã¢ãã³ãã¬ã©ã¯ããŒã¹ã¢ãã³ããã³ãã³
ããŒã¹ã¢ãã³ãªã©ãå«ããããœãŒã¹ã¢ãã³é¡ïŒã
ãã³æãšããŠãä»ã®ç³é¡ããã³ãŒã¹ããªããŒã¹ã
ãã³å€åã©ã³ããŒã¹ãªã©ãå å«ããå ±æçµŠåçå
æ°ŽçŽ æ®åºãçš®ã ã®éå«ãã§ããããŸããé«ååé
éã®èçœçæç©ãããã€ãã®è質ããã³æ žé žãå«
ãã§ããã 宿œäŸ ïŒ ç²ã¢ã¹ããã·ãã³å é§äœå«æåç»ããã®ç²Ÿè£œã¢
ã¹ããã·ãã³ã®çæïŒâ ã¢ã¹ããã·ãã³å é§äœå«æåç»ãããã«æ±æç©
ããåé¢ããã奜ãŸããå ·äœäŸãšããŠã宿œäŸïŒ
ã§åŸãç©è³ªã代衚çãªã«ã©ã ïŒ40cmïŒé·ïŒÃ2.5cm
ïŒçŽåŸïŒã196ml容ïŒãçšããŠã»ãã¢ããã¯ã¹ïŒ§â
50ã¬ãžã³äžã¯ãããã°ã©ãã€ã«ä»ãã䜿çšå§åã¯
40mmã»HgïŒæµéã¯35mlïŒHrãç·©è¡æ¶²ã¯0.05ã¢ã«
çé žç·©è¡æº¶æ¶²ïŒPH7.2ïŒã䜿çšãæåã®ãããŒã»
ã¹ã«ãŒïŒflow throughïŒã®ããŒã¯ã¯ã¢ã¹ããã·
ãã³å é§äœãšå ±ã«äžçŽç©ãå«ãããã€ã¥ãããŒã¯
ã¯äžçŽç©ã®ã¿ãå«ãã 奜ãŸããå ·äœäŸã§ã¯ãäžèšæåã®ãããŒã»ã¹ã«
ãŒã»ããŒã¯ã«ãããçæç©ãã€ãã§ãã»ãã¢ãã
ã¯ã¹ïŒ§â15äžã§æ¿çž®ããã€ãã§å®æœäŸïŒãšåãæº¶
æ¶²(1)ã(7)ãçšãã宿œäŸïŒãšåã³æº¶åºæ®µéãçšã
ãŠã»ã¬ãã¯ã¹âã®ã«ã©ã ã«éããã¢ã¹ããã·ã
ã³çæç©ã¯ãäžèšãšåã管ïŒçªå·ïŒïŒïŒãïŒïŒ
ïŒïŒå ã«ãé®®éãªããŒã¯ãšããŠååšããã»ã¬ãã¯
ã¹âã¯ãããã°ã©ãã€ãŒäžãå€éã®æ±æç©è³ªã®
ååšãªãã«ãæåããã ã€ãã§äœååéæ±æç©ãããªãã¢ãŒã»ãã€ã¹ã¯
éãªã©ã®åžžå¥ææ®µã«ããé€å»ããã奜ãŸããæ¹
æ³ãšããŠãã¢ã¹ããã·ãã³çæç©ãããªãã¢ãŒã»
ããªã³ã³ã»ãã€ã¹ã¯No.1000ã13mmïŒãã®ãã®ã¯ã
1000ãã倧ããååéãæããç©è³ªããšããã
1000ããå°ããååéãæããç©è³ªãééãã
ããïŒã«éããŠéããããšã«ãããå¡©ããã³ä»
ã®ååéã®å°ããæ±æç©è³ªãåé¢ãããã¢ã¹ãã
ã·ãã³çæç©ã¯ããªã³ã³ã»ãã€ã¹ã¯äžã«æ®çãã
宿œäŸïŒã®æº¶æ¶²(1)ã§æŽæµããããšã«ããååãã
ãã ã€ãã§ãååéçŽ8000ã®ååç©ãäžèšæº¶æ¶²ãã
åé¢ããããšã«ãã€ãŠã¢ã¹ããã·ãã³ãåŸããã
ã®æ¹æ³ãšããŠã次ã®åŠãèå±€ã²ã«ïŒTLGïŒã¯ã
ããã°ã©ããçšããã®ã奜ãŸãããè£ çœ®ãšããŠã
ããããªã³ã²ã³ã»ãã³ãã€ã GmbHïŒãã¢ãŒã
ã·ã¢ã»ãã¢ã€ã³ã»ã±ãã«ã«ãºã»ã¢ã³ãã»
CAMAGïŒã¹ã€ã¹åœïŒã«ãã€ãŠèšèšãããåžè²©ã®
ãã®ã䜿çšããããã»ãã¢ããã¯ã¹ïŒ§â200ã®ã¬
ãžã³ïŒæ¥µäžå質ã®ãã®ïŒ2.5ïœã0.02M
Na2HPO4KH2PO4äž0.5M NaClã®çé žå¡©ç·©è¡æ¶²
PH6.8ïŒ6.6ã7.0ïŒ85mlå ã«åãããå®€æž©ã§æã ã
ãããã«æ··åãã€ã€ïŒãïŒæ¥éèšæœ€ïŒSwellïŒã
ãããïŒç£æ§ããã³ãã®ä»ã®æ¹æææ®µãçšããã¹
ãã§ãªããïŒãèšæœ€ããã²ã«ãå·å枩床ã§ïŒé±éå®
å®ãããããããã现èããã³èã®å¢æ®ã¯èšæœ€ã²
ã«ãé»å®³ããããããã²ã«ãããé·æéä¿æãã
ã«ã¯ãå°éã®å¶èå€ïŒãããªãŠã ã¢ãžã0.02ïŒ ïŒ
ãå ããã¹ãã§ããã2.5ïœã®ä¹Ÿç¥ã²ã«ãçšããŠã
20Ã20cmãåã0.5mmã®ã¬ã©ã¹ã»ãã¬ãŒãïŒåã
äœãããã®ãã¬ãŒãã¯å®€æž©ã§10åé也ç¥ããæ¹¿æœ€
ãªãã€ã³ããŒå ã§çŽïŒé±é貯èµããããšãã§ãã
ãããŸãé©åœã«ãããããå¹³è¡¡ç¶æ ã«ããçŽçŽã¡
ã«äœ¿çšããããšãã§ãããïŒéåžžãå€éã«ãæåº
12æéèŠããïŒã平衡ã®äž»èŠãªæ©èœã¯ãé眮局ãšå¯
åå±€éã®å²åãæ£åžžåããããšã§ãããæ°Žå¹³äœçœ®
ã§äºåå¹³è¡¡ç¶æ ã«ãããã¬ãŒããçšããæž¬å®ãã
ãç©è³ªãããã€ã¯ãããããã§ã¹ã¿ãŒãã©ã€ã³ã«
ãããæç¹ãŸãã¯æ¡çãšããŠé©çšããã0.2ãïŒ
ïŒ ã®èçœæº¶æ¶²10ã20mlãé¡åŸ®é¡ã«ããŒã»ã¹ã©ã€ã
ïŒ18Ã18mmïŒã®ç«¯ã«èšçœ®ããã²ã«è¡šé¢ã«å¯Ÿæãã
ããæ°ç§åŸã溶液ã¯ã²ã«ã«æµžæœ€ãããå šãµã³ãã«
ããŸãã«ããŒã¹ã©ã€ãäžã«çœ®ããã€ãã§ãã°ãã
é©åãããããã䜿çšããç©è³ªãååã§ãªããšã
åé¢åŸã«åã ã®æç¹ãäœçœ®ããããããšãå°é£ã§
ããããããäœãå€ãã®ç©è³ªãé©çšãããšãæç¢º
ã«åé¢ããããšãã§ããªããæäœã容æã«ããã
ãã«ç·©è¡æ¶²ã§ãµã³ãã«ãåžéãã22ãã®è§åºŠã§ã
ã¬ãŒããåŸæãããæè¡ã§ããµã³ãã«ã®åé¢ãè¡
ããæµéã¯ïŒãïŒcmïŒæéãéåžžã«é©ããŠããã
æšèç©è³ªïŒã·ãã¯ãã ããã¢ã°ããã³ãããªã°
ããã³ãŸãã¯ããã¢ããšããŒã«ãã«ãŒã§åé¡ã»è
å¥ãããã¢ã«ããã³ïŒã¯ãã¬ãŒããæšªåã€ãŠç°ãª
ãäœçœ®ã«é©çšããããŸãæªç¥ã®ãã®ã®çžå¯Ÿçè·é¢
ïŒç§»åæ§ïŒã®èšç®ã®ããã®ç §åèçœãšããŠã®æ©èœ
ããããµã³ãã«ãé©çšããåŸããã¬ãŒããè£ çœ®å
ã«å眮ããããŒããŒã®è¯ããããäžæ¹ã«ãããŠã²
ã«å±€ãšååã«æ¥è§ŠããããããŒããŒã®è¯ã¯æµžæŒ¬ã
ããŠã¯ãªããªããéå°ã®æ°Žåã¯ã¬ããå»ãã貯ç
åšå ã®æ¶²æº¶åªã¯å®¹åšäžç«¯ããïŒcmã«äžå®ã«ä¿ã€ã
æäœã¯ãåé¢ã®é²è¡ã«äŸåããŠéåžžïŒãïŒæéã§
å®çµãããçŽåŸãæè²ç©è³ªã®åé¢ãè¡ããåé¢ã
ãèçœã®æç¹ã¯ãã¯ãããã°ã©ãã€ã®åé¢ãå®çµ
ããåŸã«ãTLGãã¬ãŒãã®ããŒããŒã·ãŒãã»ã¬
ããªã«ã«ç§»ãããšã«ãããããã³ãããããæŽæµ
ããã¡ã¿ããŒã«ïŒH2OïŒé ¢é žâ90ïŒïŒïŒïŒäžã
48æéçè²ããããšã«ããã容æã«èŠããããã«
ãªããããŒããŒã»ã·ãŒãã¯ïŒmmãçŽã§ããã20
Ã18cmã®ããŒããŒïŒæãã²ã«å±€ã®äžã«çœ®ããã²ã«
ãšé©åœã»ååãªæ¥è§Šã確å®ã«ããããã«ããã€ã
ãïŒããŒã«ããïŒãããŒããŒïŒã¬ããªã«ïŒã®äžã«
空æ°ãæãããªãããã«ããã€ãã²ã«å±€ãä¹±ããª
ãããã«æ³šæãããã²ã«å±€ããæ¶²å±€ãããŒããŒã§
浞éããçŽïŒååŸã«é€å»ããçŽã¡ã«ãªãŒãã³äžã
60âã§15åé也ç¥ããéåžžã®æè²æ³ã§æè²ããã
æè²ã¯ã10ïŒ çé žãããªãŠã äž0.03ïŒ ãžã¢ãŸåã¹
ã«ãã¢ãã«é žïŒããŠãªãŒè©Šè¬ïŒãçšããŠã¬ããªã«
âããŒããŒã«ã¹ãã¬ã€ããããšã«ãã€ãŠéæãã
ãããŸããã¡ã¿ããŒã«âé ¢é žïŒ90ïŒ10vïŒïœäœ¿
çšïŒäžãã¢ããã»ãã©ãã¯ã®é£œå溶液ãçšããŠæ
è²ãéæããããšãã§ããïŒæè²æéã¯ïŒã10å
ã§ãããè±è²ããã«ã¯ïŒå®¹éã®æ°Žãšæ··åããïŒå®¹
éã®ã¡ã¿ããŒã«ãšé ¢é žïŒ90ïŒ10ïŒãçšããŠæŽæµã
ããå€éã®æŽæµãããã«ããã¯ã°ã©ã³ããäœãæ
è²ããã®ã¯å°é£ã§ããããããã¢ã¹ããã·ãã³ã
æè²ãããå Žåã®ã¿ããã¬ãŒãèªäœãçŽ90âïŒç©º
æ°åŸªç°ææ®µãæãããªãŒãã³äžã§ïŒä¹Ÿç¥ããŠãã
ããåé¢ã®ããã«ããã¬ãŒããã宀枩ã§é¢šä¹Ÿã
ããéç±ãããšã¯ã©ãã¯ãçããããããã¯é
åžžãã»ãã¢ããã¯ã¹ïŒ§â200ãã¬ãŒãã15ã30å
ã§ä¹Ÿç¥ããæž©åºŠã50ã60âãçšããŠåé¿ããããš
ãã§ããã也ç¥ãã¬ãŒãã¯ã¡ã¿ããŒã«ïŒH2OïŒ
é ¢é žïŒ75ïŒ20ïŒïŒïŒã®æ··åç©äžã10åéèšæœ€ã
ããåãæº¶åªç³»äžé£œåã¢ãããã©ãã¯äžãïŒæé
æè²ããã€ãã§ä¹Ÿç¥ããåã«ãåãæº¶åªäžãïŒæ
éæµžæŒ¬ããããšã«ãã€ãп޿µãããååéãæ±ºå®
ããããã«ãçŽæ¥ããªã³ãïŒã¬ããªã«ïŒäžãŸãã¯
ãã³ã·ãã°ã©ã äžãã¹ã¿ãŒãã€ã³ã°ã©ã€ã³ããå
垯ã®äžå€®ãŸã§ã®è·é¢ã0.05mmã®ç²ŸåºŠã§æž¬å®ããã
枬å®çµæãäŸè©Šèçœã®ç§»åè·é¢ïŒdpïŒã«å¯Ÿãã
ã·ãã¯ãã ãŸãã¯ããªã°ããã³ïŒå¯Ÿç §èçœãšã
ãŠäœ¿çšãããïŒã®ç§»åè·é¢ïŒdmïŒã®æ¯ãšããŠå®
矩ãããRnå€ã§è¡šããããïŒäŸè©Šç©è³ªã«å¯Ÿãã
æšæºç©è³ªã®ç§»åè·é¢ã®é¢ä¿ã¯åŒïŒïŒâRnïŒdpïŒdmïŒ ã§è¡šãããããRnã«å¯ŸããŠã䜿çšãããæšæºç©
質ã®ååéã®å¯Ÿæ°ãããããããããšã«ãã€ãŠçŽ
ç·ã®ç®çå®ãç·ãåŸãããããã®çŽç·ããæªç¥ã®
èçœã®ååéãåŸããããéåžžã«æ£ç¢ºãªçµæãåŸ
ãã«ã¯ããã¬ãŒãã«é©çšããåã«ãçããããŒã
ã®èçœãµã³ãã«æº¶æ¶²ãæšæºç©è³ªããã®å Žåã¯ã·ã
ã¯ãã ïŒ£ãšæ··åããã°ãããäžèšTLGæ³ã«ããã
ã¢ã¹ããã·ãã³çæç©ã¯ãæšæºã·ãã¯ãã ãšå¯Ÿ
ç §ããŠçŽ0.83ïŒïŒâ0.02ã®è·é¢ã«åå¥ã®æç¹ãšã
ãŠèгå¯ãããã¢ã¹ããã·ãã³ã«å¯ŸããååéçŽ
8000ãåŸãããããã®æ¹æ³ã«ãããååéã®è¥å¹²
ã®çžéã«åºã¥ããŠãããã€ãã®åå¥ã®çæç©ãã¢
ã¹ããã·ãã³ããåé¢ãããããã®ããã«ããŠã
ãã®ç¹ã«æ±æç©è³ªãšããŠéã°ããååéçŽ64000ã
148000ããã³230000ãæããïŒã€ã®çæç©ããã³
ãã°ãã°ååé32000çæç©ãèŠåºãããäžèš
TLGæ³ã§é€å»ããããã¢ã¹ããã·ãã³çæç©ã¯ã
也ç¥åœ¢æ ã§ãã²ã«ã«åžãããæº¶æ¶²(1)ã«æº¶è§£ããé
å¿åé¢ãŸãã¯ä»ã®é¡äŒŒã®ææ®µã§ã¬ãžã³ãåé¢ã
ãã ãã®æ®µéã§è£œããã¢ã¹ããã·ãã³çæç©ã¯èžç
æ°Žã«å¯æº¶ã§ãããäžæ§ãé žæ§PHã§å¯æº¶ãã¢ã«ã«ãª
PHã§äžæº¶ã§ãããåå å 床èšåžåããŒã¯æ³¢é·280
ïœÎŒãæãããããã¯ãäžèšã®åŠãçŽ8000ã®åå
éãæããããªããããã§ããããã®å ±æçµåã
ãã¢ããé žã¯ã6NHClã§ã®å æ°Žåè§£ã€ãã§èªå
çãªå®é枬å®ã«ããæ¬¡ã®åŠãã¢ããé žã®å¹³åçµæ
ãæããããšã瀺ãããã æ®åºæ°ïŒçŽïŒ ã¢ã¹ãã«ãã³é ž ïŒ ãã¬ãªãã³ ïŒ ã»ãªã³ ïŒ ã°ã«ã¿ãã³é ž 13 ãããªã³ ïŒ ã°ãªã·ã³ ïŒ ã¢ã©ãã³ ïŒ ããªã³ ïŒ 1/2ã·ã¹ãã€ã³ ïŒ ã¡ããªãã³ ïŒ ã€ãœãã€ã·ã³ ïŒ ãã€ã·ã³ ïŒ ããã·ã³ ïŒ ããšãã«ã¢ã©ãã³ ïŒ ãªã·ã³ ïŒ ãã¹ããžã³ ïŒ ã¢ã«ã®ãã³ ïŒ èš 88 ã·ã¹ãã³é žãããããã·ãããªã³ããã«ãã€ã·
ã³ãã¢ã³ã¢ãã¢ãã€ãœãã¹ã¢ã·ã³ããã¹ã¢ã·ã³ã
ããããã·ãªã·ã³ããªã·ããã«ãã€ã·ã³ããã³ã¬
ã³ãâã¢ããããã«é žã¯ãã¥ããæ€åºãããéã¯
ååšãããã°ã«ã³ãŒã¹ã¢ãã³ã埮éååšããã 宿œäŸïŒã«ãããåºçºè³è «ççµç¹11ïœãããäž
èšæ¹æ³ã§ç²Ÿè£œã¢ã¹ããã·ãã³çŽïŒmgãåŸãããã 宿œäŸ 2A âãªãŒã©ãŒïŒREELERïŒâã¬ã³ã°ãã³ã®çæïŒ
â ãªãŒã©ç ã¯éºäŒã«ããç æ°ã§ãããåç©ã¯å®å®
ãªèª¿æŽã®ãšããéåçèã®æŽ»åãè¡ãããšãã§ã
ããç¹å®ã®å±éæéã«ãããŠããç¥çµçްèãè³ã®
ç¹å®ã®äœçœ®ãå°è³ãžç§»åããããšãã§ããããªãŒ
ãªã³ã°ç¶æ ãåãããé»åé¡åŸ®é¡ã§ã®ç ç©¶ã«ãã
ã°ãç¹å®ã®ç¥çµè 现èãåçŽã®æ£ç¶è»žãäžããã
ãã«æ²¿ã€ãŠæ°ãã现èããæ£åžžãªç¶æ ã«ãããŠæ°
ããäœçœ®ã«ç§»åãããããšã瀺ãããããªãŒã©ãŒ
ç ã§ãæ°ããç¥çµçްèããããã®ç¥çµè ç¹ç¶ãç»
ãããšãã§ããªãããšã¯ç¥çµçްèãããã¯ç¥çµè
ãŸãã¯ãã®äž¡æ¹ã«ãããããã€ãã®é害ã«ãããš
èããããã 宿œäŸïŒããã³ïŒã®æ¹æ³ã«åŸããããŠã¹ã®ãªãŒ
ã©ãŒè³ããã®ã¬ã³ã°ãã³ãåŸãæ£åžžãªããŠã¹ã®è³
ãã補ããã¬ã³ã°ãã³ãšæ¯èŒãããæ£åžžãªããŠã¹
ã®è³ã®å šåãã補ããã¬ã³ã°ãã³ã®ååéã¯8000
ã§ãããâãªãŒã©ãŒâã¬ã³ã°ãã³ã®ååéã¯3600
ã5000ã§ãããâãªãŒã©ãŒâãªã³ã°ãã³ã¯ããã®
éåžžã«å°ããååéã«ãã€ãŠç€ºãããããã«ç°åžž
ã§ãããããã«ããªãŒã©ãŒããŠã¹ã®è³ã®å°è³ãã
補ãåŸãã¬ã³ã°ãã³ã®éã¯æ£åžžãªãã®ãšå¯Ÿæ¯ãã
ãšã倧ãã«å°ããããã衚ã«ç€ºãã
ã³ã°ãã³ïŒRecogninsïŒãšç§°ããïŒã«é¢ãããã¬
ã³ã°ãã³ã¯ãéåžžã®ãŸãã¯ç°åžžãªçްèãŸãã¯çµ
ç¹ãäŸãã°è «ç现èãŸãã¯äººçºçãªç现èãåŠç
ããææã®ç©è³ªãåé¢ããŠè£œãããããã¬ã³ã°ã
ã³ã¯ããããã®ååŠé¢é£ç©è³ªã補ããããã«äœæ¶²
ãæããæ¯æç©ã«ã¬ã³ã°ãã³ãæ¥è§Šããããšã«äœ¿
çšããããšãã§ããããããã®ååŠé¢é£ç©è³ªã¯ã
蚺æããã³æ²»çç®çããªãã¡çã蚺æãããæ²»ç
ããã®ã«æçšãªãã®ã§ããããã®ååŠé¢é£ç©è³ª
ã¯ãçäœäžãããã¯è©Šéšç®¡äžãäŸãã°å®éçæ²
éããªãŒããããã€ïŒOuchterlonyïŒè€æ¡æ£ãã
ãã¯å ç«è¢å æ§ã«ãããŠå ç«ååŠçç¹æ§ã§ã¬ã³ã°
ãã³ãšåå¿ããç©è³ªã§ããã æ¬çºæã«ãããã¬ã³ã°ãã³ã®ïŒã€ã¯ã¢ã¹ããã·
ãã³ïŒAstrocytinïŒã§ãããã¢ã¹ããã·ãã³ã¯è³
è «ççµç¹ã奜ãŸããã¯è³ç¥çµè è «çµç¹ããåŸãã
ããã¢ã¹ããã·ãã³åå ç©ãå«ãèçœè³ªæåã¯æ
åã¯çްèçµç¹ããæœåºãããã奜ãŸããæœåºæ¹æ³
ã¯ãã¢ã¹ããã·ãã³åé§ç©ãå«ãèçœè³ªæåãæº¶
è§£ããããã«ãå質æ¡ä»¶äžã«äžæ§ç·©è¡ç©ãšçµç¹ãš
ãåŠçããããããã¯çްèããã³çµç¹ãåè§£ãã
ããšã§ããã ãã®ç¹ã§ã¢ã¹ããã·ãã³åé§ç©ã¯ãŸã ãèçœ
質ãç³èçœè³ªãèèçœè³ªãæ žé žãæ žèçœè³ªçãå«
ãååéã®å€§ããªç©è³ªã§ããããã®åŸæº¶è§£ããè
çœè³ªã¯çæããçµç¹æœåºç©ããåé¢ããããçµç¹
ããã®æœåºæº¶æ¶²ã¯ç²Ÿè£œããäžæº¶æ§ç²åãé€å»ãã
ããååéã®å°ããæ±æç©ã¯ãåŸãããæº¶æ¶²ãã
èžçºæ¿çž®æè¡ã«ãã€ãŠé€å»ããããåŸãããæº¶æ¶²
ã¯ãã®åŸåŠçãããïŒãïŒã®ç¯å²ã®pKãæãã
èçœè³ªãåŸãããã«ä»ã®æ±æç©ããã¢ã¹ããã·ã
ã³åå ç©ãåè§£ãããããã®åŸããã®æº¶æ¶²ã¯äŸã
ã°ã¯ãããã°ã©ãã€ã«ã©ã ã«èŒçœ®ãããé žæ§æº¶åª
ã§æº¶é¢ããããäžæ§ãŸãã¯pK4以äžã®é žã§æº¶é¢ã
ããèçœè³ªæåã¯ãã¹ãŠæšãŠãããïŒãïŒç¯å²ã®
pKãæãããããã®èçœè³ªæåã¯åéãããã
æº¶åºæ¶²ã¯ãã®åŸåŠçãããçŽ8000ã®ååéãæã
ãç©è³ªãåŸããããããã¯äŸãã°ãææããŸãã
ã€ã«ã¿ãŒã«ãããŠãååéã®å°ããç©è³ªããªãã¡
ååé1000以äžã®ç©è³ªãå»ããå床ãã€ã«ã¿ãŒ
ã«ãããŠçŽ25000以äžã®ç©è³ªãå»ããŠéæãã
ãã1000ã25000ã®ç¯å²ã®ååéãæããèçœè³ª
æåã¯ãã®åŸããã«èå±€ã²ã«ïŒTLGïŒã¯ããã
ã°ã©ãã€ã«ãã€ãŠåŠçãããã¢ã¹ããã·ãã³ãåŸ
ãããã ãã®ããã«ã¢ã¹ããã·ãã³ã¯ãåäžããã³é«é
ã®é å¿åé¢ãããè¿ãããšã«ãã€ãŠè³ç¥çµè è «ã
äžæ§ç·©è¡ç©ã§æœåºããåŸãããæœåºç©ããçŽïŒã
ïŒã®ç¯å²ã®pKãæããèçœè³ªæåãåé¢ããã
ã®èçœè³ªæåããé«ååéããªãã¡çŽ230000以äž
ã®ååéãæããç©è³ªãåé¢ããããããçŽ8000
ã®ååéãæããã¢ã¹ããã·ãã³ãåé¢ããããš
ã«ãã€ãŠã補ããããšãã§ããã äžèšã®æ¹æ³ã«ãã€ãŠè£œããããã¢ã¹ããã·ãã³
ã¯ãå®éçæ²éçŽ ãã¹ãããã³ãªãŒããããã€ã²
ã«æ¡æ£ãã¹ãã«ãããŠç¹æã®æäœãæããåäžç·
ã®æ²éç©ã圢æããé žæ§ãŸãã¯äžæ§PHãæããæ°Ž
溶液ããã³æ°Žææº¶æ¶²ã«å¯æº¶ã§ãããã¢ã«ã«ãªæ§PH
ã§ã¯é£æº¶ã§ãããåå å 床èšã«ããåžåããŒã¯æ³¢
é·ã280ïœÎŒã§ãããçŽ8000ã®ååéãæããŠã
ãããšãç¹åŸŽãšããŠããã ã¢ã¹ããã·ãã³ã®ç¹åŸŽã¯ãŸããã°ã«ã¿ãã³é žã
ãã³ã¢ã¹ãã©ã®ã³é žã®æ®æž£ã®å²åãããããŠé«
ãããã¹ããžã³ã«å¯Ÿãããããã®é žã®å²åããã
ããŠé«ãããšã§ãããããã«ã¢ã¹ããã·ãã³ã®å
æã以äžã«ç€ºãã äžèšãšåæ§ã«ããŠãããªã°ãã³ïŒMaligninïŒ
ãšç§°ããããä»ã®ã¬ã³ã°ãã³ã¯ã人çºçãªç现è
ããªãã¡è©Šéšç®¡äžã§åºæ¿ãåããŠæé·ããç现è
ãã補ãããããããªã°ãã³ã¯ãçŽ10000ã®åå
éãæããã¢ã¹ããã·ãã³ãšã»ãŒé¡äŒŒããŠããã
ã¢ããé žæ®æž£æåãç°ãªããããªãã¡ããªã°ãã³
ã¯ãã°ã«ã¿ãã³é žããã³ã¢ã¹ãã©ã®ã³é žã®å²åã
é«ããã€ãã¹ããžã³ã«å¯Ÿãããããã®é žã®å²åã
é«ããããã«ããªã°ãã³ã®åæã以äžã«ç€ºãã ããªãã¡ãããªã°ãã³ã¯ãçºé µå¹é€èäžã§æé·
ãã人çºçãªç现èãåäžããã³é«éã®é å¿åé¢
ãããè¿ãããšã«ãã€ãŠäžæ§ç·©è¡ç©ã§æœåºããåŸ
ãããæœåºç©ããçŽïŒãïŒã®ç¯å²ã®pKãæãã
èçœè³ªæåãåé¢ãããã®èçœè³ªæåããé«åå
éããªãã¡çŽ230000以äžã®ååéãæããç©è³ªã
åé¢ããããããçŽ10000ã®ååéãæããç©è³ª
ãåé¢ããããšã«ãã€ãŠã補ããããšãã§ããã 人çºçãªçްèå¹é€ã§è£œããããããªã°ãã³ã®é
ããã³ããªã°ãã³ã§ãã人çºç现èçºé µã§è£œãã
ããå šèçœè³ªã®å²åã¯ã倧ããªå¹é€å®¹åšäžã§ã®äºº
çºçãªç现èå¹é€èã®æé·ã«ãã€ãŠå¢å€§ããã ãã®æ¹æ³ã§è£œããããããªã°ãã³ã¯ãå®éçæ²
éçŽ ãã¹ãããã³ãªãŒããããã€ã²ã«æ¡æ£ãã¹ã
ã«ãããŠç¹æã®æäœãæããåäžç·ã®æ²éç©ã圢
æããé žæ§ãŸãã¯äžæ§PHãæããæ°Žæº¶æ¶²ããã³æ°Ž
ææº¶æ¶²ã«å¯æº¶ã§ãããã¢ã«ã«ãªæ§PHã§ã¯é£æº¶ã§ã
ããåå å 床èšã«ããåžåããŒã¯æ³¢é·ã280ïœÎŒ
ã§ãããçŽ10000ã®ååéãæããŠããããšãç¹
城ãšããŠããã ã¬ã³ã°ãã³ã®ç¹åŸŽã¯ããã«ãããã ã¢ã»ãã«ã»
ã«ããŒãºãšè€åããŠããã ã¢ã»ãã«ã»ã«ããŒãºâ
ã¬ã³ã°ãã³ã圢æããããšãå¯èœã§ãããåºä¹³å
ç©ã«æ³šå°ããéã«ç¹æã®æäœã§ããæã¬ã³ã°ãã³
ãçãããã®æã¬ã³ã°ãã³ãæ¬æ¥ã®äœçœ®ã§ã¬ã³ã°
ãã³åå ç©ã«ç¹ã«ä»çããããšã§ãããäŸãã°ã
ããçš®ã®æã¬ã³ã°ãã³ãæããªã°ãã³ã¯è©Šéšç®¡äž
ã«ãããŠè³è «ç现èã«æ¯æ§ã瀺ããã ã¢ã¹ããã·ãã³ãããªã°ãã³ããã³ãããã«é¡
䌌ããç©è³ªã®åŠãã¬ã³ã°ãã³ã¯ãçç©åŠç³»ã«å°å ¥
ãããŠãããç©è³ªãšããŠæçšã§ãã€ãŠãææãã¬
ã³ã°ãã³ã§è¢«èŠãããåŠãç°è³ªãªåå¿ãæžå°ãã
ããšãã§ãããçç©åŠç³»ã«ååŠé¢é£ç©è³ªãçãã
ããã«ã¬ã³ã°ãã³ã®ä»ã®å®äŸãå°å ¥ããããšãã§
ãããã¬ã³ã°ãã³ã¯ãŸããç¹å¥ãªçç©åŠç³»ã®æé·
ãæ é€çã«ä¿é²ããã®ã«äœ¿çšãããŠããããã¬ã³
ã°ãã³ã®æçšæ§ã¯ãŸããçç©åŠç³»ã«ãããé©å¿æ§
ãä¿é²ããããã«æ äœãæããã¬ã³ã°ãã³ã®è€å
ç©ããæãã¿ãŒã²ããïŒTargetïŒå€ã補é ã§ã
ãããšã«ããããããŠäŸãã°ãã®ã¬ã³ã°ãã³è€å
ç©ã¯ã¬ã³ã°ãã³èªäœã®ç©ççååŠçç¹æ§ãæããŠ
ãããæ äœã¯ãã¬ã³ã°ãã³ãšè€åç©ã圢æãããš
ãšãã«çç©åŠçã«å®è³ªçã«äžæŽ»æ§ã§ããç©è³ªãã
éžå®ãããã¹ãã§ããã ããªãããããŸãã¯èçœè³ªãšå®å®è€åç©ã圢æ
ãããããªãå ¬ç¥ç©è³ªãã¬ã³ã°ãã³ãšè€åããã®
ã«æçšã§ããããã®äžäŸãšããŠããã ã¢ã»ãã«â
ã»ã«ããŒãºã®åŠãã»ã«ããŒãºæããããçç©åŠç³»
ã«äžæŽ»æ§ã§ããããšã«å ããŠããã®æ äœã¯ãæ¬æ
现æžã§è¿°ã¹ãç®çã«æçšãªã¬ã³ã°ãã³ã®ç¹æã®ç©
ççååŠçæ§è³ªã倿Žããªããã®ã§ãªããã°ãªã
ãªãã ã¬ã³ã°ãã³ãšãã®æ äœãšããæãè€åç©ã¯ãæ¥
è§Šãããçç©åŠç³»ã®ååŠé¢é£ç©è³ªã補é ããåé¢
ãã確èªããã®ã«æçšã§ãããã¬ã³ã°ãã³âæ äœ
è€åç©ã¯ãŸããå°å ¥ãããçç©åŠç³»ã®ååŠé¢é£ç©
質åå ç©ã®è£œé ãä¿é²ããã®ã«æçšã§ããã ååŠé¢é£ç©è³ªã®ïŒé¡ã¯ãæã¬ã³ã°ãã³ããªãã¡
æã¢ã¹ããã·ãã³ããã³æããªã°ãã³ã§ãããã
ããã®ç©è³ªã¯çç©åŠç³»ã«ã¬ã³ã°ãã³ã泚å°ããŠè£œ
ããããšãã§ãããã¬ã³ã°ãã³ã®å ç«åŠçã«æå¹
ãªæçšéã¯ãåŸæ¥ã®æäœè£œé æè¡ã«åŸã€ãŠæäœå
å¿ãèªçºããããã«äœå çµç¹ãŸãã¯äœæ¶²ãšæ¥è§Šã
ããŠç®åºããããæã¬ã³ã°ãã³ã¯ãæã¬ã³ã°ãã³
ãæããè€å圢å€ãçç©åŠç³»ã«å°å ¥ããŠæãçç©
åŠç³»ã®ç¹ç°çްèãŸãã¯äœçœ®ã«èšºæå€ãæ é€å€ãã
ã³æ²»çå€ã®åŠãç©è³ªãæäžããã®ã«äœ¿çšããããš
ãã§ããããã®æã¬ã³ã°ãã³ã¯ãŸããçè²ãŸãã¯
æŸå°æ§åé¡è¡šç€ºãè «ç现èã«ã®ã¿çããããã«ã
ææããã³æŸå°æ§ç©è³ªã®åŠãåé¡è¡šç€ºç©è³ªãé å
ããæã¬ã³ã°ãã³ãçäœçµç¹ã«é©çšããããšã«ã
ã€ãŠãçäœçµç¹ã«è «ç现èãååšãããåŠãã蚺
æããã®ã«æçšã§ãããããã«æã¬ã³ã°ãã³ã䜿
çšããã®ã¯ãã¬ã³ã°ãã³ã®å ç«çã«æå¹ãªæçšé
ãåºä¹³åç©ãã®ä»ã®çç©åŠç³»ã«æ³šå°ããããšã«ã
ã€ãŠãä»ã®æçšãªååŠé¢é£ç©è³ªïŒäŸãã°TAGã
åŸè¿°ïŒã®åºä¹³åç©ããç£åºé«ãå¢å ããããã§ã
ãã åŸæ¥ãç³èçœè³ªè€åç©ã¯ãè³çµç¹ãã補é ã
ããæäœãè³çµç¹ã«åœ¢æãããŠãããã¿ã€ãµãã¹
ïŒTayâSachsïŒã®ç çãªè³ãã補ãããã10Bç³
èçœè³ªãšããŠç¥ãããåé¢ç©è³ªã¯ãã©ããããã
ã³è£œããããæäœã«æ³šå°ãããŠããããããã®ã¿
ã€ãµãã¹æäœã¯ãè «çãæããè³ã®å ç«è¢å æ§ç
ç©¶ã«äœ¿çšãããŠãããè «çã°ãªã¢ã§ã¯ãªãåå¿ç
ãªæ®éã®éè «çã°ãªã¢ã®ã¿ããããã®æäœã«ãã€
ãŠçè²ãããŠããã ãããšå¯Ÿç §çã«ãã¢ã¹ããã·ãã³ãè «ççµç¹ã
ã補ããããæãã¢ã¹ããã·ãã³ã«å¯Ÿããæäœ
ïŒæã¢ã¹ããã·ãã³ïŒã補ããããè³ã®å ç«è¢å
æ§ç ç©¶ã«äœ¿çšãããæ®éã®ïŒéè «çïŒã°ãªã¢ã§ã¯
ãªãè «çã°ãªã¢ã®ã¿ãæã¢ã¹ããã·ãã³ã«ãã€ãŠ
çè²ãããŠããããããã€ãŠãåå çµç¹ãæäœã®
æ§è³ªããã³çè²ããã现èç¹æã®ã¿ã€ãã«ãã€
ãŠãæã¿ã€ãµãã¹æäœããã³æã¢ã¹ããã·ãã³ã¯
ç°ãªãç©è³ªã§ããã®ã¯æããã§ããã ååŠé¢é£ç©è³ªã®ä»ã®é¡ã¯ããããã®ååŠé¢é£ç©
質ãšè€åããã¿ãŒã²ããå€ã§ãããäŸãã°ãã¿ãŒ
ã²ããïŒããã ã¢ã»ãã«âã»ã«ããŒãºã®åŠãæ äœ
ãšè€åããã¢ã¹ããã·ãã³ç©è³ªïŒã¯æã¢ã¹ããã·
ãã³ãšã®æ¥è§Šã«äœ¿çšãããããã®ã¿ã€ãã®ååç©
ã¯ãè€åãããŠãçç©åŠç³»ã®ç¹ç°çްèãŸãã¯äœçœ®
ã«èšºæå€ãæ é€å€ããã³æ²»çå€ãæäžããããã«
䜿çšããããšãã§ããããããã®ååç©ã¯ãŸãæŽ
æµåŠçã®ããã«ã䜿çšã§ãããäŸãã°ãæã¢ã¹ã
ãã·ãã³ã¯ãããã ã¢ã»ãã«ã»ã«ããŒãºâã¢ã¹ã
ãã·ãã³âæã¢ã¹ããã·ãã³ãé žãŸãã¯ãããã€
ããŒãŒé µçŽ ã§å æ°Žåè§£ããŠè£œããããšãã§ããã
ã¿ãŒã²ããå€ã¯ãŸããã¿ãŒã²ããã®å ç«åŠçã«æ
å¹ãªæçšéãäœå çµç¹ãŸãã¯äœæ¶²ã«æ¥è§Šããããª
ã©ã«ãã€ãŠãçç©åŠç³»ã®TAGç©è³ªïŒåŸè¿°ïŒã®é
ãå¢å ããã®ã«åœ¹ç«ã€ã ä»å çãªååŠé¢é£ç©è³ªã¯TAGå€ïŒã¿ãŒã²ãã
ã«ä»çããã°ãããªã³ïŒã§ãããTAGç©è³ªã¯ã
TAGãè€ååè§£ããããã®æéãå€åãããã
ã«ã¿ãŒã²ããå€ãäœæ¶²ãšæ¥è§ŠãããŠåŸãããã äºã€ã®æçšãªå®æœæ æ§ã¯ïŒ³âTAGåã³ïŒŠâ
TAGã§ããã âTAGïŒSLOWâTargetâAttachingâ
GlodulinïŒã®è£œé æ¹æ³ã¯ãè¡æž ãã®ä»ã®äœæ¶²ãæš
çïŒå³ã¡ãããã¢ã¢ã»ãã«ã»ã«ããŒãºâããªã°ã
ã³ïŒãšçŽïŒæéåã¯ãã以äžãäœæž©äŸãã°çŽïŒâ
ã§åå¿ãããâTAGãçæç©ããéè£ããã
ãšãäŸãã°çŽ37âã®æž©åºŠã§çŽïŒæéçšéé žã§éè£
ããããšããæãããã®æ¹æ³ã«åŸã€ãŠè£œé ããã
補åâTAGã¯æ°Žæ§ç·©è¡æ¶²ã«å¯æº¶ã§ãããã¢ãŠ
ãã¿ãŒãããŒïŒOuchterlonyïŒã²ã«æ¡æ£è©Šéšã«ã
ããŠãã®çžåœã¬ã³ã°ãã³ãšã®åç³»æ²æŸ±ç©ãçæ
ããã»ããã¢ã³èã§éæäžèœã§ããã25000åå
é以äžã®ååãä¿æããããªãã¢âãã€ã«ã¿ãŒã§
çä¿ããããã¯ãã»ã°ãããªã³åãçŽ50000åã³
ãã®åæ°ã®èå±€ã²ã«ã»ã¯ãããã°ã©ãã€ãŒã«ãã€
ãŠå®ãããããããªç°ã€ãç¶æ ã®ååºãããåå
éãæãããããŠ280ïœÎŒã®åå å 床èšåžåããŒ
ã¯æ³¢ãæããããšãç¹åŸŽãšããã âTAGïŒFastâTargetâAttachingâ
GlobulinïŒã補é ããæ¹æ³ã¯ãè¡æž ãã®ä»ã®äœæ¶²
ãæšçïŒå³ã¡ãããã ã¢ã»ãã«ã»ã«ããŒãºâããª
ã°ãã³ïŒãšçŽ10åéäœæž©äŸãã°ïŒâã§åå¿ããã
âTAGãçæç©ããéè£ããããšãäŸãã°çŽ
37âã®æž©åºŠã§çŽïŒæéçšéé žã§éè£ããããšãã
æãããã®æ¹æ³ã«åŸã€ãŠè£œé ããã補åâ
TAGã¯æ°Žæ§ç·©è¡æ¶²ã«å¯æº¶ã§ãããã¢ãŠãã¿ãŒã
ããŒïŒOuchterlonyïŒã²ã«æ¡æ£è©Šéšã«ãããŠãã®
çžåœã¬ã³ã°ãã³ãšã®åç³»æ²æŸ±ç©ãçæããã»ãã
ã¢ã³èã§éæäžèœã§ããã25000ååé以äžã®å
åãä¿æããããªãã¢ã»ãã€ã«ã¿ãŒã§çä¿ããã
ãã¯ãã»ã°ãããªã³åããçŽ50000åã³ãã®åæ°
ã®èå±€ã²ã«ã»ã¯ãããã°ã©ãã€ãŒã«ãã€ãŠå®ãã
ãããããªç°ã€ãç¶æ ã®ååºãããååéãæ
ãããããŠ280ïœÎŒã®åå å 床èšåžåããŒã¯æ³¢é·
ãæããããšãç¹åŸŽãšããã TAG補åã¯æ¢ç¥éã®åºä¹³åç©ã®è¡æž ãã®ä»ã®
äœæ¶²ã«ãã€ãŠäœãããâTAGåã³ïŒŠâTAGã®
æ¿åºŠã決å®ããŠãã®æ¿åºŠãšãçã瀺ããã®ãšããŠ
å®ããããå€ãšãçžé¢ãããããšã«ãã€ãŠãçã
ãåºä¹³åç©ã®çè «çãæ¢ç¥ããã®ã«æçšã§ããã
TAG補åã¯ãŸãçµç¹éšäœã®è «ç现èã®ååšã蚺
æããã®ã«æçšã§ããããããã¯ææãšãæŸå°æ§
ç©è³ªã®ãããªæšèç©è³ªãšæ¥åããTAGãåœè©²éš
äœã«é©çšããããšããæããããã«ãã€ãŠè «ç现
èã«ã®ã¿çè²åã¯æŸå°æ§æšèãçãããã®ã§ã
ããæŽã«TAG補åã¯è «ç现èã«å¯ŸããŠçŽ°èæ¯æ§
ãããããšã倿ãããTAG補åã¯ãŸãã蚺æã
æ é€ãåã³æœççšã®å€ãTAG補åãšé¯ç¶ã«ãã
ãã®ãå°å ¥ããããšã«ãã€ãŠããããã®å€ãç¹å®
ã®çްèåã¯éšäœã«å±ãããã®ã«æçšã§ããã 现èãå®å šã«ç¹å®ã®å Žæãå ããããã«ãªã
ãšãæ€ç©åã¯åç©ã«ãããæ£åžžã®çްèåå²ã¯å¶é
ããããåã¯ææ¢ãããã(a)æ£åžžã®çްèããèªå·±
ã®å ãåŸãå Žæãåããããšããèªèãããæ©æ§
åã³(b)ãã®èªèæ©æ§ã®äœçšãä»åºŠã¯çްèã®åå²ã
ææ¢ããæ©æ§ã¯ãå ±ã«ç¥ãããŠãããæ£åžžã®èªè
åã³æç¥ãçãããšãå é§ç©è³ªã®æ¿åºŠãå¢å€§ãã
ç²ååã³çްèã®èªèåã³æç¥ã«é¢ä¿ãããããªäž
矀ã®ååç©ã§ãã€ãŠçްèãäºãã«é£çµãããã®ã
補é ãããããã«ãªã€ãããããã®ååç©ã¯ã¬ã³
ã°ãã³ãºãšåŒã°ãããéåžžã®ç现èãããããã®
ååç©ã補é ããããšè©Šã¿ããšããããããã¯ã
ã®ãããªãã®ãšããŠã¯ãªãããšããããããŸãã
ç现èã(a)ãã®æ£åžžãªäœç©ãå ããããšãèªèã
ãèœåãåã³ïŒåã¯(b)ãã®æ£åžžãªäœç©ãå ããé
ã«åå²ãæ¢ãããšããèœåãã倱ããšåæã«ãã®
ååæ§é ã«å€åãçããããšãããã€ãã æ°èŠãªååç©ãªãã³ã«ãã®ãããªååç©ã補é
ããæ¹æ³ãçºèŠãããããããã®æ°èŠååç©ã¯ã¬
ã³ã°ãã³ãºãšåä»ãããããã¬ã³ã°ãã³ãºã¯ã现
èåå²ãèªèãæ¢ããããšãã§ããªããšããç¹ã§
ç现èã®åœ¢æ çç¹æ§ãæš¡å£ããç©çååŠçç¹æ§ã
æããæ°èŠååç©ã§ãããã¬ã³ã°ãã³ã®äœ¿çšã¯ç
ã®äœç𿩿§ãèŠãããã以äžã«ãããããã®ã§ã
ããäœæ ãªãã°ããã«ãã€ãŠçã®èšºæåã³æ²»çãª
ãã³ã«ãã®äºé²ã«åœ¹ç«ã€çŽæ¥è£œåãªãã³ã«æ¹æ³ã
æäŸãããããã§ããã ããªã°ãã³ãºïŒMALIGNINSïŒã補é ããã®
ã«äººå·¥çã«å¹é€ãã现èãçšããããšãã§ããæ¹
æ³ãçºèŠããããããã«é瀺ããæ¹æ³ã®å©ç¹ã®äž
ã€ã¯ãããªã°ãã³ãºïŒMALIGNINSïŒåã³ãã
ããäœãæ°è£œåãäºå®äžç¡å¶éã®éã§å¹ççã«è£œ
é ããããšãã§ãããšããããšã§ããã æ¬çºæã¯çç ç©¶ã®åéã«åªããã®ã§ããããŸã
ãã¹ãŠã®çé·åã³æ°é³ä»£è¬ã«åœ±é¿ãäžããããšæ
ãäžåã®çç©åŠç³»ã«çŽã¡ã«é©çšã§ãããã®ã§ã
ãããããã«ã人工çå¹é€ããé©åœãªçްèåã®ç¹
å®ã®ååç©ã補é ããããšã«ããããŸããããã®
ç©è³ªãã補åãæŽã«è£œé ããããšã«ãããå ãæ
åã«çç©ç³»ã«ãããçµç¹ã现èãçŽ°èæ©èœè³ªã次
æ©èœè³ªååãããã¯ååéåäœã«ç¹å®ã®åœ±é¿ãå
ãŒãããšãã§ããããããããŠãçºè²æã®éèŠæ
æã«ãããç¹å®ã®æ é€å¹åãç¹å®ã®èšºæäžã®ãäº
é²äžã®ãåã³æ²»çäžã®æ¹æ³ãåã³äººå·¥ç黿°ã·ã¹
ãã ã®æ§æïŒçµç¹åã¯åšç®¡ç§»æ€ã«ãããåŠãïŒã
ãã¹ãŠæåã«åœ±é¿ãåããããšãã§ãããããã
ã®äººå·¥ç黿°ç³»ã¯ä»ããããã«é£ããããšããªã
éåžžã®çµç¹åã¯çµæã®ç¹å®ã®ã¬ã³ã°ãã³
ïŒRECOGNINïŒãããªã°ãã³ïŒMALIGNINïŒå
ã¯ãããã®ã±ã¢ã¬ã·ããã«ã«ãº
ïŒCHEMORECIPROCALSïŒã®ç¹æ§ãæã€ããã«
ããããšãã§ããã®ã§ããç°ç©ããšããŠãèªèã
ãããããããæçµ¶ãå«ãç°ç©ã«å¯Ÿããåå¿ãé¿
ãããã®ã§ããã æ¬çºæã®ä»ã®æ æ§ã¯ãç¹å®ã®é è³è£œåïŒã¢ã¹ã
ãã·ãã³ïŒã«å¯Ÿãã䟡å€ã®é«ãç¹å®ã®æäœç¶ã®è£œ
åïŒã¢ã³ãã»ã¢ã¹ããã·ãã³ïŒã補é ããããšã§
ããããã®æäœç¶è£œåãããããçš®ã®ç¥çµç³»ã®ç¹
å®ã®ç¹ã«ç¹å®ã«é¯ç¶ãšãªãããã«ããããŠããã«
察ããç¹å®ã®äŒéæ äœãšããŠçšããããšãã§ãã
ããšã§ãããããªã°ãã³ãºïŒMALIGNINSïŒå
ã³ã¢ã¹ããã·ãã³ïŒASTROCYTINïŒã¯ã¬ã³ã°
ãã³ãºïŒRECOGNINSïŒã§ããã æ¬çºæã®æŽã«ä»ã®æ æ§ã¯ãäºã€ã®æ°è£œåã®çç©
åŠçæµäœããTARGETâATTACHINGâ
GLOBULINSïŒTAGïŒã補é ããããšã§ããã
ããã¯äºã€ã®åå¿å³ã¡ç¬¬äžã¯çç©åŠçæµäœãããª
ã°ãã³ãºïŒMALIGNINSïŒãæš¡å£ããç©çååŠ
ç圢æ ãå«ãåæé¯äœãšåå¿ããããã®ã§
TARGETãšåŒã°ãã第äºã¯ç¹å®ã®TAGãé¯äœ
ããéè£ããããã®ã§ããã®ææ©äœã«è «çããã
ãã©ããçããææ©äœã®çç©åŠçæµäœããã®å®é
ç衚瀺ãåŸãŠãå³ã¡è «çã®èšºæè©Šéšããããã®ã
ãã«äœãããTAGã®å¯žæ³ã«ãã€ãŠãã®ããã«åœ
åããããã®ã§ãããTAG補ååã³ANTIâ
MALIGNINã¯ç©çååŠçã«MALIGMINãè£ãª
ããã®ã§ããããããããã¯
CHEMORECIPROCALSãšåœåãããã æŽã«ãè¡æž ãšäœ¿çšããç¹å®ã®TARGET詊è¬ãš
ã®åå¿ã«èš±ãããæéã«ãã€ãŠããŸãé¯åããã
補åã®éè£ã«èš±ãããæéã«ãã€ãŠãäºã€ã®å®é
çåã³å®æ§çã«æãã«åºå¥ãããTAG補åã補
é ããããšãã§ããããšãçºèŠãããã è³è «çã®ãããåã³çš®ã ã®ä»ã®å»åŠçæ éã®ã
ãããªãã³ã«ãªããæãããªçŸç ã®åŸŽåã®ãªãå€
æ°ã®ç°ã€ã人ã ãã補é ããããšãã§ããããã
ã®è£œåã®éãæ€æ»ããåŸãäžå®ã®åºäººã«ã€ããŠè£œ
é ããããšãã§ãããããã®äºã€ã®æ°èŠè£œåã®é
ã¯ããã®å人ã«è³è «çããã€ããã©ããã瀺ãã
ã®ã§ããããšãæãããšãªã€ããããããè³è «ç
ã®æ°èŠè¡æž 蚺æè©Šéšæ³ãçºèŠãããã®ã§ããã ãããã®æ°èŠè£œåã®è¡æž ãã®ä»ã®çç©åŠçæµæ¶²
ããè³ãã®ä»ã®è «çã蚺æããçšéã®ã»ãã®å¹çš
ã¯ãTAGåã³æRECOGNINååç©ã¯è³è «çã®
å€ç§æè¡ã§åé€ãããè³è «çåã³ãã®åšå²ã®çµç¹
ã®çµç¹åŠçéšäœã«ãããŠåªå çã«ç¥çµè è «ç现è
ã«ã€ããšãã蚌æ ã§èª¬æãããããã®è «ç现èã®
TAGåã³æRECOGNINSã«ããåªå çæšèã¯æš
æºå ç«è¢å æè¡ã«ãã€ãŠèšŒæãããããã®ãã
ã«ãæ°èŠæ¹æ³ã¯ãŸããçµç¹åŠçæ€æ»ã«ãã€ãŠæ°èŠ
ãªç¢ºåºŠããã€ãŠãè «ç现èãé€å»ããçµç¹ã®æ¥µç«¯
éšãŸã§æµžéããŠãããã©ãããæ±ºå®ããã®ã«çšã
ãããšãã§ããè «çãè³ãã®ä»ã®åšå®ã«ãŸã æ®ã€
ãŠããå¯èœæ§åã¯è «ç现èãé€å»ããçµç¹ã®åšèŸº
ãããªããªã€ãŠããå¯èœæ§ã瀺ããè «çã®ãã¹ãŠ
ãè³åã¯ä»ã®åšå®ããåé€ãããå¯èœæ§ã瀺ãã
ãšãã§ãããæŽã«ãèšè¿°ããããã«è£œé ãã
TAGåã³æMALIGNINã¯è©Šéšç®¡å ã§çµç¹å¹é€
äœã«çæããè è «è³è «ç现èã«å¯ŸããŠçŽ°èæ¯æ§ã
ããããšãããã€ããããã§ã¯çµç¹å¹é€äœã«çæ
ããããä»ã®åªäœã§ã®è «ç现èã«å¯Ÿãããã®é«èŠª
åæ§ã¯ãæ°è£œåTAGã®ç¹å®çµåæœåšåãæŽã«èšŒ
æãããã®ã§ãããåæè£œåTARGETã«é¢ããŠ
åã³çµç¹åŠçéšäœã«ãããè «ç现èã«é¢ããŠ
TAGã®æ§è³ªã衚ããããã«TARGETâ
ATTACHINGâGOBULINSïŒTAGïŒã®åç§°ã®
æ¡çšã説æãããã®ã§ãããæŽã«ãè «ç现èã«å¯Ÿ
ããTAGåã³æRECOGNINSã®çŽ°èæ¯æ§ã¯è «ç
ã«ããã€ãçãã®ããæ£è ã®è¡æž ã®æŽã«æ°ãã蚺
æè©Šéšæ³ãæäŸããããã®ããã«ãäŸãã°ããã
ãã®æ£è ã®è¡æž åã¯ä»ã®äœæ¶²ãTARGETãšåå¿
ãããŠTAGã補é ãã補åTAGã¯çµç¹å¹é€äœäž
ã§è «ç现èã®çé·ãçŽ°èæ¯æ§æ€æ»ãããäžå®ã®å
人ã®è¡æž ãã補é ããããšãã§ããTAGã«ãã€
ãŠçŸããããTAGã®æ¿åºŠåã³çŽ°èæ¯æ§åºŠã¯åã«
蚺æäžã®å©ç¹ãããã°ããã§ãªããç¹å®ã®æ£è ã«
ã€ããŠã®æè¡ååã³æè¡åŸã®äžèª¿ã®çµéãæ¢çŽ¢ã
ãäžã«ã䟡å€ã®ãããã®ã§ãããæŸå°æ§åã³æè²
æ¢ç¥ç©ãTAGãšçµåããã°ãè «çã®èšºæåã³ã
ã®æ£ç¢ºãªéšäœã®å€å®ãããå Žåçäœã«æçšã§ãã
æ°èŠãªTAG補åãäœãããšãã§ããããããã«
ããŠãåèå åã¯éèå ã®ããããã«é©åœã«æšè
ããTAGãè³è髿¶²å ã«ãåã¯çŽæ¥è³çµç¹åã¯
ãã®çª©ã«æ³šå°ããã°ãæŸå°æ§ææ®µã«ãããåã¯çµ
åããææã®èŠèŠåã«ãããè³è «çã®ååšã説æ
ããããšãã§ããããšããã®ã¯TAGãç¹å¥ã«ä»
çããã®ã¯è «ç现èã«ã®ã¿ã§ããããã§ãããæŽ
ã«ããã®æ¹æ³ã¯è³è «çã®éšäœãæ£ç¢ºã«èŠèŠåãã
ããšãã§ãããããã¯è³è «çãæšèããããå ã®
è¡æ¶²ã§äœã€ãæASTROCYTINãçšãããã®ç
äœå èšºææ¹æ³ã®æ¹è¯ãšèŠãªãããšãã§ããããªã
ãªãã°äººéã®è¡æž ããäœã€ãTAGãçšããã°ç°
èçœåå¿ã®å¯èœæ§ãé¿ããããããã§ããã
TAGåã³æRECOGNINSã¯è©Šéšç®¡å åã³çäœå
ã®åæ¹ã§ã®è «ç现èãå«ãASTROCYTINåé§
ç©è³ªã«åªå çã«ä»çããããšãã§ããååŠçç¹æ®
æ§ãæã€ã®ã§ãäŸãã°æŸå°æ§ã®ããããã³è£ç²å€
åã¯ä»ã®æ¯æ§ã®ããç©ççåã¯ååŠçå€ãšçµåã
ããŠããããã®æ¯æ§ã®ããç©è³ªãè «ç现èã«é£æ¥
ããæ£åžžçŽ°èãšæ¯èŒããŠè «ç现èã«ä»çããç¹æ®
æ§ã«ãã€ãŠåªå çã«å±åšåããããšãã§ãããã
ã«ã蚺æåŠçã«ãšåããæœççã«ããããã®è£œå
ãçšããããšãã§ããããã®éžææ§ã¯ãè «çã®å¹
æçãªååŠçåã¯ç©ççæœçãéæããããã®æ±º
å®çãªãå°ããšãäžã€ã®æ±ºå®çãªèŠçŽ ãšããŠããŸ
ããããŸã§éæãããªãã€ãèŠçŽ ãšããŠãæ®éç
ã«èªããããããããã«ãTAGã¯åªå çã«è «ç
现èã«ä»çããç¹ã§å¹æã®ããããšã蚌æããã
ã®ã§ããããããã®çç±ã§æ°èŠãªæ²»ç補åãšããŠ
ã®æææ§ãæã€ã¹ããã®ã§ããã æªæ§ã®è «çãæã€ãæ£è ã®è¡æž ã«ãããŠã¯ãäž
èšã®å®æœäŸã«èŠãããããã«ãFASTâTAGïŒïŒŠ
âTAGïŒãšåºå¥ãããäžã€ã®åã®TAGãå³ã¡
SLOWâTAGïŒïŒ³âTAGïŒããããããªè «çã®
ãªãæ£è ã«ãããããããäžå®éã®è¡æž ããæ¯èŒ
çå€éã«è£œé ããããšãã§ããããã®ããšã¯ã
TAGã®èªç¶ã«çºçããåé§ç©è³ªïŒïŒ°âTAGïŒã®
ããããäžã€ã®æ¿åºŠãå¢å€§ãããåã¯ïŒŠâTAG
ãããâTAGãæ¯èŒç詊éšç®¡å ã§è¯ãåºæ¥ã
ãããªä»ã®èŠå ãããããšã瀺åãããã®ã§ã
ãã å®éã®åæè£œåTARGETåã³TAGã®ãã®å
é§ç©è³ªã«å¯ŸããããããŠãäºããããã°ãçäœå
ã«ååšããããšãããåŸããããã«å¯Ÿããä»®å®ã
ããã蚌æãããŠãªã现èãæåãåã³åŸªç°ãã
ãæäœãã®äœçšã«å¯Ÿãããäœçšã®ããåŸãé¢ä¿ã¯ã
ãŸã è§£æãããŠããªãããã®ããã«äŸãã°ãæäœ
ç¶ã®æ§åŒã§ãâTAGåã³ïŒ³âTAGã¯ã¢ãŠãã¿
ãŒãããŒã»ã²ã«æ¡æ£ã«ãããŠASTROCYTINãš
åäžã§å¥ã ã®ç³»çµ±ã®åå¿ãçæãããããã§ãå
ã«TARGETãæ³šå°ããã°ãTARGETãšåå¿ã
ãåŸãå ã®è¡æž ããäœãTAG補åã®åçãå¢ã
ããããšã«ãªãã埪ç°ããæäœã«äŒŒãåé§ç©è³ªã
åå²ããªã现èã«é ãããŠãã现èæåã«å¯ŸããŠ
æ£åžžãªæ°ŽæºãããåŸããšããçºèŠã¯ãäžå¯Ÿã®ã
ã®ãããåŸãäœçšã«ã€ããŠã®åé¡ãæèµ·ããã
ããã§ã现èã®å¢æ®åã³çްèã®æ»æ» ã®å¶åŸ¡ã«äœçš
ããTAGåé§ç©è³ªïŒïŒ°âTAGïŒåã³TARGET
ç¶ç©è³ªãçäœå ã«ååšããããšãæèµ·ããããã
ãã«ãäŸãã°ãéåžžã¯çŽæ¥ã¯è¡æž èçœã«æããã
ããšã®ãªãçŽ°èæ§æç©ã®é²åºã现èåå²äžã«èµ·ã
ããšãããããã®çŽ°èæ§æç©ã®é²åºã®çµæãæ§æ
ç©ã¯TARGETç¶ç©è³ªã«å€æãããããã«ãªãã
ããã«è¡æž ããâTAGç¶ååã®ä»çãçãã
ããã现èåå²ãåºæãããåã¯ææ¢ããããšã
èããããããããã¯ãå·å®³ãåããåã¯äœçšäž
è¯ã®äžåå²çްèã¯ïŒ°âTAGç¶ååã®ä»å ãå埩
å¯èœã§ãããããªTARGETç¶ç©è³ªãé²åºããã
ããšãããããããããã现èç¶æ ã®äžã§ã¯ïŒ°â
TAGç¶ååã®ä»å ã¯çްèã®ç Žå£ãæãããšãã
ãïŒäŸãã°ããã«èšèŒããåæã§è£œé ããANTI
âGLIOMAâTAGã¯çµç¹å¹é€äœäžã§çé·ããè
è «ç现èã«èããçŽ°èæ¯æ§ãããïŒããããã«ã
ããã¯ã现èåå²ã®å¶åŸ¡ã«ã€ããŠããŸãææ©äœã®
çæ¶¯ãéããŠäœå ã®åã ã®çްèã®ä¿®åŸ©åã¯é€å»ã«
ã€ããŠã®æ£åžžãªæ©æ§ã®æš¡ç¯ã瀺ããã®ãšããã
ããè³å è è «ã®å Žåã®ããã«æ¥éã«åå²ããç现
èã«çããããã«ãçŽ°èæ§æç©ã®é²åºãç°åžžã«å¢
倧ããŠç°åžžã«å€§éã®çްèTARGETç¶ç©è³ªã圢æ
ãããå Žåã¯ãäžã€ã®åã®è¡æž âTAGã®æ¿åºŠ
ãä»ã®ãã®ã«æ¯ã¹ãŠå¢å€§ããããšãèµ·ãããšãã
ãã åèšç©è³ªã®å®éã®äœçšãã©ã®ãããªãã®ã§ãã
ããšãããã«èšè¿°ããæ¹æ³ã«ãã€ãŠæªæ§ã®è «çã®
ããæ£è ã®è¡æž ãã詊éšç®¡å ã§è£œé ããããšãã§
ããTAGã®äž»èŠãªäžã€ã®åãSLOWâTAGïŒïŒ³
âTAGïŒã®çžåœéã®å¢å ã¯ãäžèšã®å®æœäŸã«èš
èŒããè¡æž 蚺æè©Šéšã®åºç€ãšãªããã®ã§ããã 宿œäŸ ïŒ ç²ã¢ã¹ããã·ãã³å é§äœå«æåç»ã®çæïŒâ 人éã®è³è è «è «è¹çµç¹ãåãåãã衚é¢ã®è¡ç®¡
ãšæ®éã®è³çµç¹ãšãå¯èœãªéãåé¢ããããåé¢
ããè «è¹çµç¹ã®ä»£è¡šçãªé11ïœããšããããã
1.5ïœã®éšåïŒåããã³1.0ïœã®éšåïŒåã«åã
ããã€ãã§åéšåãæ¬¡ã®ããã«åŠçããã åéšåãäžæ§ç·©è¡æº¶æ¶²äžãè¶ é³æ³¢ãŸãã¯ä»ã®æ©
æ¢°çææ®µã«ããå質åãããããšãã°ãåéšåã
ã¯ãŒãã³ã°æ··åæ©äžã0.005Mçé žç·©è¡æ¶²ïŒPHïŒïŒ
ã®100c.c.ïŒïœçµç¹ã«å質åãããèçœã®å€æ§ãé²
ãããã«ãäžèšå質ååŠçã¯å·æ¡ä»¶äžã«è¡ãã§ã
ã§ãããããšãã°ãè©²æ··åæ©ãïŒãïŒâã®å·å®€ã§
ãããããå·ãããçŽïŒåéã§æäœãã¹ãã§ã
ãã ã€ãã§ãåçè³ªãæž æŸã«ããããã«ãããšãã°
å·åããè¶ é å¿åé¢ã§80000åéåã30åéäœçš
ãããŠé å¿åé¢ãããã坿º¶æ§äžæŸæ¶²ãåŸæåé¢
ããå·äžã«ä¿ã€ãäžæº¶æ§æ®æž£ã¯ãããã«äžæ§ç·©è¡
æ¶²100mlãçšããŠåã³å質åããäžèšãšåæ§ã«é
å¿åé¢åŠçããåŸãããïŒçªç®ã®å¯æº¶æ§æœåºç©ãš
äžèšïŒçªç®ã®ãããšåãããäžæŸæ¶²äžãèçœã50
ãã€ã¯ãã°ã©ã ïŒml溶液以äžãšãªããŸã§å質åãš
é å¿åé¢ãããããããšããåçã¯éåžžã«è¯å¥œã§
ããããã®ããšã¯ãã»ãšãã©ã®çµç¹ã®å ŽåãïŒå
ã®æœåºã§éæãããã ãã®ããã«ããŠåŸã溶液ãåããã€ã¥ãéæã§
ååã«èžçºãããããšã«ããæ¿çž®ãããå·äžã
0.006Mçé žç·©è¡æ¶²ã®éæã«ãã15mlã®æº¶æ¶²ãåŸ
ãããã®æº¶æ¶²ã®éãèšé²ããäžéšãå šèçœã®åæ
ã«çšããæ®äœãåããŠpK1ãïŒã®èçœè³ªåç»ãåŸ
ãã以äžã«è¿°ã¹ãã¯ãããã°ã©ãã€ã¯å¥œãŸããå
奿³ã§ããã æº¶æ¶²ããå·å®€ïŒïŒâïŒäžã0.005Mçé žãããª
ãŠã ç·©è¡æ¶²ã§å¹³è¡¡ãããDEAEã»ã«ããŒã¹
ïŒCellexâïŒã«ã©ã 2.5Ã11.0cmäžã§åå¥ããã
æº¶åºæº¶åªã¯ä»¥äžã®æº¶åªïŒæº¶æ¶²ïŒãçšããããšã«ã
ãæ®µéçã«å€ããïŒæº¶æ¶²(1)NaH2PO44.04ïœãã
ã³Na2HPO46.50ïœãèžæºæ°Ž15000mlã«æº¶è§£ãã
ïŒ0.005ã¢ã«ãPHïŒïŒïŒæº¶æ¶²(2)NaH2PO48.57ïœãèž
æºæ°Ž2480mlã«æº¶è§£ããïŒæº¶æ¶²(3)NaH2PO417.1ïœ
ãèžæºæ°Ž2480mlã«æº¶è§£ããïŒ0.05ã¢ã«ãPH4.7ïŒïŒ
溶液(4)NaH2PO459.65ïœãèžæºæ°Ž2470mlã«æº¶è§£ã
ãïŒ0.175ã¢ã«ïŒïŒæº¶æ¶²(5)NaH2PO4101.6ïœãèžæº
æ°Ž2455mlã«æº¶è§£ããïŒ0.3ã¢ã«ãPH4.3ïŒïŒæº¶æ¶²(6)
NaH2PO4340.1ïœãèžæºæ°Ž2465mlã«æº¶è§£ãã
ïŒ1.0ã¢ã«ãPH4.1ïŒïŒæº¶æ¶²(7)80ïŒ çé žïŒH3PO4ïŒ
283.64ïœãèžæºæ°Ž2460mlã«å ããïŒ1.0ã¢ã«ãPH
1.0ïŒ ç¥çµçµç¹æœåºç©ïŒã10mlãå ããããããã«ã©
ã ã«éããã€ãã§æº¶æ¶²(1)ãäžã«å ããæº¶æ¶²(1)300
mlãåãã貯çåšãåä»ããŠéåã§ã«ã©ã ã«èœäž
ããããèªåçãªåç»åéè£ çœ®ãçšããŠæº¶åºæ¶²ïŒ
mlãéãããã€ãã§ãæ¬¡ã®æº¶åºç®¡çªå·ã§ãæº¶åºæº¶
æ¶²ãå€ããŠçšãããæº¶æ¶²(2)ïŒç®¡ïŒïŒã§ãã«ã©ã äž
溶液ãã¬ãžã³ã®é éšã«éã³ãã€ãã§æº¶æ¶²(2)50mlã
äžããå ã貯æºåšãåä»ããïŒç®¡ïŒïŒã§ã溶液ã
ã«ã©ã äžãã¬ãžã³ã®é éšã«éã³ãã€ããŠã溶液(3)
75mlãäžããå ãã貯çåšãåä»ããïŒæº¶æ¶²(4)ïŒ
管ïŒïŒïŒã§ã溶液ãã«ã©ã äžãã¬ãžã³ã®é éšã«é
ã³ãã€ãã§æº¶æ¶²(4)150mlãäžããå ãã貯çåšã
åä»ããïŒæº¶æ¶²(5)ïŒç®¡ïŒïŒïŒã§ã溶液ããã«ã©ã
äžãã¬ãžã³ã®é éšã«ãã€ãŠãããã€ãã§æº¶æ¶²(5)ã
äžããå ãã貯çåšãåä»ããïŒæº¶æ¶²(6)ïŒç®¡ïŒïŒ
ïŒã§ã溶液ãã«ã©ã äžãã¬ãžã³ã®é éšã«ãã€ãŠã
ããã€ãã§æº¶æ¶²(7)175mlãäžããå ãã貯çåšã
åä»ããïŒç®¡ïŒïŒïŒã«å°ããŸã§æº¶åºãç¶ããŠæº¶åº
ãå®äºãããçµç¹æœåºç©ã®å容ããšã«æ°ãã«è£œã
ãã¬ãžã³ã䜿çšãããå管ãèçœã®å®éåæã«ä»
ãã管ïŒïŒïŒãïŒïŒïŒå ã®æº¶åºæ¶²ãåããããã¯
ç²çæç©ãå«ã¿ãããããã¢ã¹ããã·ãã³ãåŸã
ããã ãã®éå»ã«ãããŠãã©ã¯ã·ãšã³10BãšåŒã°ãã
ç²çæç©ã«ã€ããŠã®ããŒã¿ã¯å ¬ã«ãããŠããã
ããããã€ã³ã»ã¡ã¿ããªãºã ã»ãªãã»ã¶ã»ããŒã
ã¹ã»ã·ã¹ãã pp555ã69ïŒPleum Pressã1970
幎ïŒïŒãžã€ãŒãã«ã»ãªãã»ãã€ããµãŒãžã€ãªãŒã
Vol.33ãpp281ã286ïŒ1970å¹ŽïŒæïŒåç §ïŒãããã
ã§ã¢ã¹ããã·ãã³ãšåŒã°ããç¹å®ã®çæç©ãåç»
ïŒïŒïŒ¢ããåé¢ããããšãéæãããç²åç»ïŒïŒ
ã¯ãã¯ããã®æ°é®®ãªç¥çµç³»çµç¹ïŒïœã«ã€ã0.1
ã10mgã®çæç©ãšããŠè£œãããããããã¯ã¢ã¹ã
ãã·ãã³å é§äœã®ä»ã«ã倿°ã®ãããœã·ã¹ãããª
ãã¡ã°ã«ã³ãŒã¹ãã¬ã©ã¯ããŒã¹ããã³ããŒã¹ïŒã°
ã«ã³ãŒã¹ã¢ãã³ãã¬ã©ã¯ããŒã¹ã¢ãã³ããã³ãã³
ããŒã¹ã¢ãã³ãªã©ãå«ããããœãŒã¹ã¢ãã³é¡ïŒã
ãã³æãšããŠãä»ã®ç³é¡ããã³ãŒã¹ããªããŒã¹ã
ãã³å€åã©ã³ããŒã¹ãªã©ãå å«ããå ±æçµŠåçå
æ°ŽçŽ æ®åºãçš®ã ã®éå«ãã§ããããŸããé«ååé
éã®èçœçæç©ãããã€ãã®è質ããã³æ žé žãå«
ãã§ããã 宿œäŸ ïŒ ç²ã¢ã¹ããã·ãã³å é§äœå«æåç»ããã®ç²Ÿè£œã¢
ã¹ããã·ãã³ã®çæïŒâ ã¢ã¹ããã·ãã³å é§äœå«æåç»ãããã«æ±æç©
ããåé¢ããã奜ãŸããå ·äœäŸãšããŠã宿œäŸïŒ
ã§åŸãç©è³ªã代衚çãªã«ã©ã ïŒ40cmïŒé·ïŒÃ2.5cm
ïŒçŽåŸïŒã196ml容ïŒãçšããŠã»ãã¢ããã¯ã¹ïŒ§â
50ã¬ãžã³äžã¯ãããã°ã©ãã€ã«ä»ãã䜿çšå§åã¯
40mmã»HgïŒæµéã¯35mlïŒHrãç·©è¡æ¶²ã¯0.05ã¢ã«
çé žç·©è¡æº¶æ¶²ïŒPH7.2ïŒã䜿çšãæåã®ãããŒã»
ã¹ã«ãŒïŒflow throughïŒã®ããŒã¯ã¯ã¢ã¹ããã·
ãã³å é§äœãšå ±ã«äžçŽç©ãå«ãããã€ã¥ãããŒã¯
ã¯äžçŽç©ã®ã¿ãå«ãã 奜ãŸããå ·äœäŸã§ã¯ãäžèšæåã®ãããŒã»ã¹ã«
ãŒã»ããŒã¯ã«ãããçæç©ãã€ãã§ãã»ãã¢ãã
ã¯ã¹ïŒ§â15äžã§æ¿çž®ããã€ãã§å®æœäŸïŒãšåãæº¶
æ¶²(1)ã(7)ãçšãã宿œäŸïŒãšåã³æº¶åºæ®µéãçšã
ãŠã»ã¬ãã¯ã¹âã®ã«ã©ã ã«éããã¢ã¹ããã·ã
ã³çæç©ã¯ãäžèšãšåã管ïŒçªå·ïŒïŒïŒãïŒïŒ
ïŒïŒå ã«ãé®®éãªããŒã¯ãšããŠååšããã»ã¬ãã¯
ã¹âã¯ãããã°ã©ãã€ãŒäžãå€éã®æ±æç©è³ªã®
ååšãªãã«ãæåããã ã€ãã§äœååéæ±æç©ãããªãã¢ãŒã»ãã€ã¹ã¯
éãªã©ã®åžžå¥ææ®µã«ããé€å»ããã奜ãŸããæ¹
æ³ãšããŠãã¢ã¹ããã·ãã³çæç©ãããªãã¢ãŒã»
ããªã³ã³ã»ãã€ã¹ã¯No.1000ã13mmïŒãã®ãã®ã¯ã
1000ãã倧ããååéãæããç©è³ªããšããã
1000ããå°ããååéãæããç©è³ªãééãã
ããïŒã«éããŠéããããšã«ãããå¡©ããã³ä»
ã®ååéã®å°ããæ±æç©è³ªãåé¢ãããã¢ã¹ãã
ã·ãã³çæç©ã¯ããªã³ã³ã»ãã€ã¹ã¯äžã«æ®çãã
宿œäŸïŒã®æº¶æ¶²(1)ã§æŽæµããããšã«ããååãã
ãã ã€ãã§ãååéçŽ8000ã®ååç©ãäžèšæº¶æ¶²ãã
åé¢ããããšã«ãã€ãŠã¢ã¹ããã·ãã³ãåŸããã
ã®æ¹æ³ãšããŠã次ã®åŠãèå±€ã²ã«ïŒTLGïŒã¯ã
ããã°ã©ããçšããã®ã奜ãŸãããè£ çœ®ãšããŠã
ããããªã³ã²ã³ã»ãã³ãã€ã GmbHïŒãã¢ãŒã
ã·ã¢ã»ãã¢ã€ã³ã»ã±ãã«ã«ãºã»ã¢ã³ãã»
CAMAGïŒã¹ã€ã¹åœïŒã«ãã€ãŠèšèšãããåžè²©ã®
ãã®ã䜿çšããããã»ãã¢ããã¯ã¹ïŒ§â200ã®ã¬
ãžã³ïŒæ¥µäžå質ã®ãã®ïŒ2.5ïœã0.02M
Na2HPO4KH2PO4äž0.5M NaClã®çé žå¡©ç·©è¡æ¶²
PH6.8ïŒ6.6ã7.0ïŒ85mlå ã«åãããå®€æž©ã§æã ã
ãããã«æ··åãã€ã€ïŒãïŒæ¥éèšæœ€ïŒSwellïŒã
ãããïŒç£æ§ããã³ãã®ä»ã®æ¹æææ®µãçšããã¹
ãã§ãªããïŒãèšæœ€ããã²ã«ãå·å枩床ã§ïŒé±éå®
å®ãããããããã现èããã³èã®å¢æ®ã¯èšæœ€ã²
ã«ãé»å®³ããããããã²ã«ãããé·æéä¿æãã
ã«ã¯ãå°éã®å¶èå€ïŒãããªãŠã ã¢ãžã0.02ïŒ ïŒ
ãå ããã¹ãã§ããã2.5ïœã®ä¹Ÿç¥ã²ã«ãçšããŠã
20Ã20cmãåã0.5mmã®ã¬ã©ã¹ã»ãã¬ãŒãïŒåã
äœãããã®ãã¬ãŒãã¯å®€æž©ã§10åé也ç¥ããæ¹¿æœ€
ãªãã€ã³ããŒå ã§çŽïŒé±é貯èµããããšãã§ãã
ãããŸãé©åœã«ãããããå¹³è¡¡ç¶æ ã«ããçŽçŽã¡
ã«äœ¿çšããããšãã§ãããïŒéåžžãå€éã«ãæåº
12æéèŠããïŒã平衡ã®äž»èŠãªæ©èœã¯ãé眮局ãšå¯
åå±€éã®å²åãæ£åžžåããããšã§ãããæ°Žå¹³äœçœ®
ã§äºåå¹³è¡¡ç¶æ ã«ãããã¬ãŒããçšããæž¬å®ãã
ãç©è³ªãããã€ã¯ãããããã§ã¹ã¿ãŒãã©ã€ã³ã«
ãããæç¹ãŸãã¯æ¡çãšããŠé©çšããã0.2ãïŒ
ïŒ ã®èçœæº¶æ¶²10ã20mlãé¡åŸ®é¡ã«ããŒã»ã¹ã©ã€ã
ïŒ18Ã18mmïŒã®ç«¯ã«èšçœ®ããã²ã«è¡šé¢ã«å¯Ÿæãã
ããæ°ç§åŸã溶液ã¯ã²ã«ã«æµžæœ€ãããå šãµã³ãã«
ããŸãã«ããŒã¹ã©ã€ãäžã«çœ®ããã€ãã§ãã°ãã
é©åãããããã䜿çšããç©è³ªãååã§ãªããšã
åé¢åŸã«åã ã®æç¹ãäœçœ®ããããããšãå°é£ã§
ããããããäœãå€ãã®ç©è³ªãé©çšãããšãæç¢º
ã«åé¢ããããšãã§ããªããæäœã容æã«ããã
ãã«ç·©è¡æ¶²ã§ãµã³ãã«ãåžéãã22ãã®è§åºŠã§ã
ã¬ãŒããåŸæãããæè¡ã§ããµã³ãã«ã®åé¢ãè¡
ããæµéã¯ïŒãïŒcmïŒæéãéåžžã«é©ããŠããã
æšèç©è³ªïŒã·ãã¯ãã ããã¢ã°ããã³ãããªã°
ããã³ãŸãã¯ããã¢ããšããŒã«ãã«ãŒã§åé¡ã»è
å¥ãããã¢ã«ããã³ïŒã¯ãã¬ãŒããæšªåã€ãŠç°ãª
ãäœçœ®ã«é©çšããããŸãæªç¥ã®ãã®ã®çžå¯Ÿçè·é¢
ïŒç§»åæ§ïŒã®èšç®ã®ããã®ç §åèçœãšããŠã®æ©èœ
ããããµã³ãã«ãé©çšããåŸããã¬ãŒããè£ çœ®å
ã«å眮ããããŒããŒã®è¯ããããäžæ¹ã«ãããŠã²
ã«å±€ãšååã«æ¥è§ŠããããããŒããŒã®è¯ã¯æµžæŒ¬ã
ããŠã¯ãªããªããéå°ã®æ°Žåã¯ã¬ããå»ãã貯ç
åšå ã®æ¶²æº¶åªã¯å®¹åšäžç«¯ããïŒcmã«äžå®ã«ä¿ã€ã
æäœã¯ãåé¢ã®é²è¡ã«äŸåããŠéåžžïŒãïŒæéã§
å®çµãããçŽåŸãæè²ç©è³ªã®åé¢ãè¡ããåé¢ã
ãèçœã®æç¹ã¯ãã¯ãããã°ã©ãã€ã®åé¢ãå®çµ
ããåŸã«ãTLGãã¬ãŒãã®ããŒããŒã·ãŒãã»ã¬
ããªã«ã«ç§»ãããšã«ãããããã³ãããããæŽæµ
ããã¡ã¿ããŒã«ïŒH2OïŒé ¢é žâ90ïŒïŒïŒïŒäžã
48æéçè²ããããšã«ããã容æã«èŠããããã«
ãªããããŒããŒã»ã·ãŒãã¯ïŒmmãçŽã§ããã20
Ã18cmã®ããŒããŒïŒæãã²ã«å±€ã®äžã«çœ®ããã²ã«
ãšé©åœã»ååãªæ¥è§Šã確å®ã«ããããã«ããã€ã
ãïŒããŒã«ããïŒãããŒããŒïŒã¬ããªã«ïŒã®äžã«
空æ°ãæãããªãããã«ããã€ãã²ã«å±€ãä¹±ããª
ãããã«æ³šæãããã²ã«å±€ããæ¶²å±€ãããŒããŒã§
浞éããçŽïŒååŸã«é€å»ããçŽã¡ã«ãªãŒãã³äžã
60âã§15åé也ç¥ããéåžžã®æè²æ³ã§æè²ããã
æè²ã¯ã10ïŒ çé žãããªãŠã äž0.03ïŒ ãžã¢ãŸåã¹
ã«ãã¢ãã«é žïŒããŠãªãŒè©Šè¬ïŒãçšããŠã¬ããªã«
âããŒããŒã«ã¹ãã¬ã€ããããšã«ãã€ãŠéæãã
ãããŸããã¡ã¿ããŒã«âé ¢é žïŒ90ïŒ10vïŒïœäœ¿
çšïŒäžãã¢ããã»ãã©ãã¯ã®é£œå溶液ãçšããŠæ
è²ãéæããããšãã§ããïŒæè²æéã¯ïŒã10å
ã§ãããè±è²ããã«ã¯ïŒå®¹éã®æ°Žãšæ··åããïŒå®¹
éã®ã¡ã¿ããŒã«ãšé ¢é žïŒ90ïŒ10ïŒãçšããŠæŽæµã
ããå€éã®æŽæµãããã«ããã¯ã°ã©ã³ããäœãæ
è²ããã®ã¯å°é£ã§ããããããã¢ã¹ããã·ãã³ã
æè²ãããå Žåã®ã¿ããã¬ãŒãèªäœãçŽ90âïŒç©º
æ°åŸªç°ææ®µãæãããªãŒãã³äžã§ïŒä¹Ÿç¥ããŠãã
ããåé¢ã®ããã«ããã¬ãŒããã宀枩ã§é¢šä¹Ÿã
ããéç±ãããšã¯ã©ãã¯ãçããããããã¯é
åžžãã»ãã¢ããã¯ã¹ïŒ§â200ãã¬ãŒãã15ã30å
ã§ä¹Ÿç¥ããæž©åºŠã50ã60âãçšããŠåé¿ããããš
ãã§ããã也ç¥ãã¬ãŒãã¯ã¡ã¿ããŒã«ïŒH2OïŒ
é ¢é žïŒ75ïŒ20ïŒïŒïŒã®æ··åç©äžã10åéèšæœ€ã
ããåãæº¶åªç³»äžé£œåã¢ãããã©ãã¯äžãïŒæé
æè²ããã€ãã§ä¹Ÿç¥ããåã«ãåãæº¶åªäžãïŒæ
éæµžæŒ¬ããããšã«ãã€ãп޿µãããååéãæ±ºå®
ããããã«ãçŽæ¥ããªã³ãïŒã¬ããªã«ïŒäžãŸãã¯
ãã³ã·ãã°ã©ã äžãã¹ã¿ãŒãã€ã³ã°ã©ã€ã³ããå
垯ã®äžå€®ãŸã§ã®è·é¢ã0.05mmã®ç²ŸåºŠã§æž¬å®ããã
枬å®çµæãäŸè©Šèçœã®ç§»åè·é¢ïŒdpïŒã«å¯Ÿãã
ã·ãã¯ãã ãŸãã¯ããªã°ããã³ïŒå¯Ÿç §èçœãšã
ãŠäœ¿çšãããïŒã®ç§»åè·é¢ïŒdmïŒã®æ¯ãšããŠå®
矩ãããRnå€ã§è¡šããããïŒäŸè©Šç©è³ªã«å¯Ÿãã
æšæºç©è³ªã®ç§»åè·é¢ã®é¢ä¿ã¯åŒïŒïŒâRnïŒdpïŒdmïŒ ã§è¡šãããããRnã«å¯ŸããŠã䜿çšãããæšæºç©
質ã®ååéã®å¯Ÿæ°ãããããããããšã«ãã€ãŠçŽ
ç·ã®ç®çå®ãç·ãåŸãããããã®çŽç·ããæªç¥ã®
èçœã®ååéãåŸããããéåžžã«æ£ç¢ºãªçµæãåŸ
ãã«ã¯ããã¬ãŒãã«é©çšããåã«ãçããããŒã
ã®èçœãµã³ãã«æº¶æ¶²ãæšæºç©è³ªããã®å Žåã¯ã·ã
ã¯ãã ïŒ£ãšæ··åããã°ãããäžèšTLGæ³ã«ããã
ã¢ã¹ããã·ãã³çæç©ã¯ãæšæºã·ãã¯ãã ãšå¯Ÿ
ç §ããŠçŽ0.83ïŒïŒâ0.02ã®è·é¢ã«åå¥ã®æç¹ãšã
ãŠèгå¯ãããã¢ã¹ããã·ãã³ã«å¯ŸããååéçŽ
8000ãåŸãããããã®æ¹æ³ã«ãããååéã®è¥å¹²
ã®çžéã«åºã¥ããŠãããã€ãã®åå¥ã®çæç©ãã¢
ã¹ããã·ãã³ããåé¢ãããããã®ããã«ããŠã
ãã®ç¹ã«æ±æç©è³ªãšããŠéã°ããååéçŽ64000ã
148000ããã³230000ãæããïŒã€ã®çæç©ããã³
ãã°ãã°ååé32000çæç©ãèŠåºãããäžèš
TLGæ³ã§é€å»ããããã¢ã¹ããã·ãã³çæç©ã¯ã
也ç¥åœ¢æ ã§ãã²ã«ã«åžãããæº¶æ¶²(1)ã«æº¶è§£ããé
å¿åé¢ãŸãã¯ä»ã®é¡äŒŒã®ææ®µã§ã¬ãžã³ãåé¢ã
ãã ãã®æ®µéã§è£œããã¢ã¹ããã·ãã³çæç©ã¯èžç
æ°Žã«å¯æº¶ã§ãããäžæ§ãé žæ§PHã§å¯æº¶ãã¢ã«ã«ãª
PHã§äžæº¶ã§ãããåå å 床èšåžåããŒã¯æ³¢é·280
ïœÎŒãæãããããã¯ãäžèšã®åŠãçŽ8000ã®åå
éãæããããªããããã§ããããã®å ±æçµåã
ãã¢ããé žã¯ã6NHClã§ã®å æ°Žåè§£ã€ãã§èªå
çãªå®é枬å®ã«ããæ¬¡ã®åŠãã¢ããé žã®å¹³åçµæ
ãæããããšã瀺ãããã æ®åºæ°ïŒçŽïŒ ã¢ã¹ãã«ãã³é ž ïŒ ãã¬ãªãã³ ïŒ ã»ãªã³ ïŒ ã°ã«ã¿ãã³é ž 13 ãããªã³ ïŒ ã°ãªã·ã³ ïŒ ã¢ã©ãã³ ïŒ ããªã³ ïŒ 1/2ã·ã¹ãã€ã³ ïŒ ã¡ããªãã³ ïŒ ã€ãœãã€ã·ã³ ïŒ ãã€ã·ã³ ïŒ ããã·ã³ ïŒ ããšãã«ã¢ã©ãã³ ïŒ ãªã·ã³ ïŒ ãã¹ããžã³ ïŒ ã¢ã«ã®ãã³ ïŒ èš 88 ã·ã¹ãã³é žãããããã·ãããªã³ããã«ãã€ã·
ã³ãã¢ã³ã¢ãã¢ãã€ãœãã¹ã¢ã·ã³ããã¹ã¢ã·ã³ã
ããããã·ãªã·ã³ããªã·ããã«ãã€ã·ã³ããã³ã¬
ã³ãâã¢ããããã«é žã¯ãã¥ããæ€åºãããéã¯
ååšãããã°ã«ã³ãŒã¹ã¢ãã³ã埮éååšããã 宿œäŸïŒã«ãããåºçºè³è «ççµç¹11ïœãããäž
èšæ¹æ³ã§ç²Ÿè£œã¢ã¹ããã·ãã³çŽïŒmgãåŸãããã 宿œäŸ 2A âãªãŒã©ãŒïŒREELERïŒâã¬ã³ã°ãã³ã®çæïŒ
â ãªãŒã©ç ã¯éºäŒã«ããç æ°ã§ãããåç©ã¯å®å®
ãªèª¿æŽã®ãšããéåçèã®æŽ»åãè¡ãããšãã§ã
ããç¹å®ã®å±éæéã«ãããŠããç¥çµçްèãè³ã®
ç¹å®ã®äœçœ®ãå°è³ãžç§»åããããšãã§ããããªãŒ
ãªã³ã°ç¶æ ãåãããé»åé¡åŸ®é¡ã§ã®ç ç©¶ã«ãã
ã°ãç¹å®ã®ç¥çµè 现èãåçŽã®æ£ç¶è»žãäžããã
ãã«æ²¿ã€ãŠæ°ãã现èããæ£åžžãªç¶æ ã«ãããŠæ°
ããäœçœ®ã«ç§»åãããããšã瀺ãããããªãŒã©ãŒ
ç ã§ãæ°ããç¥çµçްèããããã®ç¥çµè ç¹ç¶ãç»
ãããšãã§ããªãããšã¯ç¥çµçްèãããã¯ç¥çµè
ãŸãã¯ãã®äž¡æ¹ã«ãããããã€ãã®é害ã«ãããš
èããããã 宿œäŸïŒããã³ïŒã®æ¹æ³ã«åŸããããŠã¹ã®ãªãŒ
ã©ãŒè³ããã®ã¬ã³ã°ãã³ãåŸãæ£åžžãªããŠã¹ã®è³
ãã補ããã¬ã³ã°ãã³ãšæ¯èŒãããæ£åžžãªããŠã¹
ã®è³ã®å šåãã補ããã¬ã³ã°ãã³ã®ååéã¯8000
ã§ãããâãªãŒã©ãŒâã¬ã³ã°ãã³ã®ååéã¯3600
ã5000ã§ãããâãªãŒã©ãŒâãªã³ã°ãã³ã¯ããã®
éåžžã«å°ããååéã«ãã€ãŠç€ºãããããã«ç°åžž
ã§ãããããã«ããªãŒã©ãŒããŠã¹ã®è³ã®å°è³ãã
補ãåŸãã¬ã³ã°ãã³ã®éã¯æ£åžžãªãã®ãšå¯Ÿæ¯ãã
ãšã倧ãã«å°ããããã衚ã«ç€ºãã
ã衚ã
衚ã«ã¯ããªãŒã©ãŒããŠã¹ã®å°è³ã«ã¬ã³ã°ãã³
æ¿åºŠã®èããããæžå°ããããè³ã®ä»ã®éšã«åé
ãŸãã¯ããå€ãã®ã¬ã³ã°ãã³ãæãããšã瀺ãã
ãŠãããã¬ã³ã°ãã³ã¯è³ã®ä»ã®éšåããå°è³ãžç§»
åããªãããŸãã¯ããªãŒã©ãŒããŠã¹ã®è³ã®ä»ã®å
æã§è¥å¹²å¢å ããŠããããšã¯ããè£åè¡çºãåæ
ããŠããã ãªãŒã©ãŒããŠã¹ã®è³ã«ãããç çåŠçã¬ã³ã°ã
ã³ã¯ã现èå ã«ãããŠãèªç¥ã«ãããã¬ã³ã°ãã³
ã®åœ¹å²ã確ããªãã®ãšãããã€èªèããé©åœãªäœ
眮ã«äœçœ®ããããšãéæããããã®ãç§»åããèœ
现èãããªããã°ãªããªãæ¥è§Šãã§ããªãããšãš
é¢é£ããŠããã åæ§ã«ããŠãããŠã¹ã®ãªãŒã©ãŒã®è³ãã補ãã
ããã¬ã³ã°ãã³ã«å¯Ÿããæã¬ã³ã°ãã³ã補ãã
ããããã«ã®ã¹ãããã«ãæã¢ã¹ããã·ãã³ãã
ã³æã¢ããªã°ãã³ã®äœ¿çšæ³ãšåæ§ã«ããŠããŠã¹ã«
䜿çšãããŠããã 宿œäŸ ïŒ äººå·¥ã¬ã³çްèå¹å°çºé µã«ãããããªã°ãã³å é§
äœã®çæïŒâ äžè¬ã«ãæ» èåŠçã¯æ³šææ·±ãè¡ãªãããã ãã¹ãŠã®æº¶æ¶²ïŒããšãã°ããã³ã¯ã®å¹³è¡¡å¡©
ïŒBSSïŒãâ10æ é€åªäœãåçã®è±å ã®è¡æž ãã
ãªãã·ã³æº¶æ¶²ïŒãã䜿çšåã«æ°ŽæµŽäžãçŽ35âã§çŽ
20åãããã¯ãã以äžã®æéå¹é€ããã äžèšã®åŠãé©åœãªåªäœãçšããããšã«ããã现
èã®è «ççµç¹ããåé¢ããå€äžä»£ã«ããã€ãŠè©Šéš
管å ã§å¢æ®ãããæ» èæº¶æ¶²ãããšãã°ã12âãã
ãããŒã«ããã³ã¢ã ãã€ã«ãŸãã¯ã¯ã¬ãªãªã³æº¶æ¶²
ã§ãããããæŽæµããããŒã«ãçšããã 奜ãŸããå ·äœäŸãšããŠã人工ã¬ã³çްèïŒããªã
ã¡ãå€äžä»£ã®éã詊éšç®¡å ã§å¢æ®ãã现èïŒã
250ml容ãã©ã¹ã³ã§çè²ããã现èã墿®ããæ¶²
äœåªäœããããããæŽæµããããŒã«ã«å ¥ãããã€
ãã§çްèããã³ã¯ã®BSSãŸãã¯ä»ã®é¡äŒŒã®æº¶æ¶²ã
çšããçŽ30ç§ééãã«æŽæµãããæ¹æãé¿ããã
ãã¹ãŠã®å£ããã³è¡šé¢ãæŽæµãããå·äžã§çŽ10å
éã3000rpmã®é å¿åé¢ã«ããæº¶æ¶²ã现èããæž
æŸã«ãããåªäœãäžèšã®åŠããããŒã«ãŒã«æ³šãã
å°éã®ç·©è¡ãããã€ããŒãŒã»ãšã³ã¶ã€ã 溶液ãå
ãã现èã®æ¶åãé¿ããããã«ãã°ããæŽæµã
ãã奜ãŸããæ¹æ³ãšããŠãããªãã·ã³æº¶æ¶²
ïŒEDTAïŒïŒãïŒmlãå ããããã10ç§éæŽæµã
ããããªãã·ã³æº¶æ¶²ãæµåºãããã åãéã®æ°é®®ãªããªãã·ã³æº¶æ¶²ãå ããé¡åŸ®é¡
芳å¯ã§çްèããã€ã³ããŒå£ããåé¢ããã®ãèŠã
ãããŸã§å¹é€ãããããã«ã¯éåžžãïŒã10åéã
èŠãããé©åœãªå¢æ®åªäœãããšãã°ïŒŠâ10æ é€åª
äœ100mläžãåçã®èå ã®è¡æž ã®ïŒã10ïŒ æº¶æ¶²50
mlãå ããã 现èãšå ±ã«æ°é®®ãªåªäœ25mlãæ°ãã墿®çšãã€
ã³ããŒã«ç§»ããŠç¹æ®ããããäž¡ãã€ã³ããŒãå¹é€
æ©äžã35âã§çŽïŒæ¥é眮ããæ¬å®æœäŸã«ãããã
ããŸã§ã®æäœã«ããã人工ã¬ã³çްèå¹å°ã¯çŽïŒæ¥
ããšã«ïŒã€ã®æ°ããå¹å°ã«åå²ããããå墿®ã
ã€ã³ããŒã«ã€ããŠããã®å šæäœããã»ãïŒæ¥ã®é
éããããŠæãŸããé »åºŠã ãããè¿ãããã®ãã
ã«ããŠã詊éšç®¡å ã®çè²çŽ°èæ°ã¯ã»ãïŒæ¥ããšã«
ïŒåã«ãªãã çŽïŒæ¥éã®å¢æ®ã®åŸãããªã°ãã³ã補ãããã
ã«çްèãæœåºãããããšãã°ãäžèšå250ml墿®
çšãã€ã³ããŒå ã®å¢æ®çްèã¯ä»¥äžã®åŠãåçãã
ãã åªäœãé å¿åé¢ç®¡ã«ãã€ããå·äžã3000rpmã§
10åéé å¿åé¢ãããåªäœãæšãŠãã墿®çšãã€
ã³ããŒå ã«æ®ã€ãŠãã现èããã€ã³ããŒå£ããå¥
é¢ããŠãäžæ§ç·©è¡æº¶æ¶²ã§é å¿å颿©å ã«æŽãæµã
蟌ãã现èãäžæ§ç·©è¡æº¶æ¶²ã§ïŒåæŽããåã³ãå·
äžã3000rpmã§é å¿åé¢ããåªäœãæšãŠããç²ã
ãªã°ãã³å é§äœå«æåç»ã®æœåºã®ããã®æºåãã§
ãããŸã§äžæ§çé žå¡©ç·©è¡æ¶²10mläžã«æŽæµãã现è
ãæžæ¿ãããã 宿œäŸ ïŒ ç²ããªã°ãã³å é§äœå«æåç»ã®çæïŒâ 宿œäŸïŒã§è£œãããäžæ§ç·©è¡æ¶²äžã«æžæ¿ããæŽ
æµãã现èãã倧éšåã®èçœã®å€æ§ãé¿ãåŸãæ¡
ä»¶äžã«æ©æ¢°çã«ç²ç ããã奜ãŸããæ¹æ³ãšããŠã
æŽæµãã现èãå·äžãè¶ é³æ³¢ã§20ç§éåŠçããã
è¶ é³æ³¢åŠçåŸãçŽ°èæ®æž£ã30åéã30000rpmã§
é å¿åé¢ããäžæŸæ¶²ãåŸæåé¢ãããç·©è¡æº¶æ¶²10
mléšãçšããŠæ®çããçŽ°èæ®æž£ãæŽæµãããè¶ é³
æ³¢åŠçããã³é å¿åé¢åŠçãäžèšã®åŠãè¡ããäž
æŸæ¶²ãåãããã®æäœãå床ãããããã äžæŸæ¶²ãåããååã«èžçºãããŠå®¹éãçŽ30ml
ããçŽïŒãïŒmlã«ãããæšæ¬ãå šèçœåæã«çš
ããæ®éšããã¢ã¹ããã·ãã³å é§äœã«ã€ããŠã®å
èšå®æœäŸïŒã«èšèŒã®æ¹æ³ã«åŸã€ãŠåå¥ããã 宿œäŸ ïŒ ç²ããªã°ãã³å«æåç»ããã®ç²Ÿè£œããªã°ãã³ç
æç©ã®çæïŒâ ã¢ã¹ããã·ãã³ã«ã€ããŠã®å®æœäŸïŒã®æ¹æ³ã«ã
ã€ãŠããªã°ãã³çæç©ãããã«æ±æç©è³ªããåé¢
ããã 奜ãŸããå ·äœäŸã®TLG段éã§ã¯ãããªã°ãã³
çæç©ã¯æšæºã·ãã¯ãã ã«å¯Ÿæ¯ããŠçŽ0.91ïŒïŒ
â0.02ã®è·é¢ã«åå¥ã®æç¹ãšããŠèгå¯ãããããª
ã°ãã³ã«å¯ŸãååéçŽ10000ãäžããããã ãã®æ®µéã§è£œããããããªã°ãã³çæç©ã¯èžç
æ°Žã«å¯æº¶ã§ãããäžæ§ãŸãã¯é žæ§PHã§å¯æº¶ãã¢ã«
ã«ãªPHã§äžæº¶ã§ãããåå å 床èšçåžåããŒã¯
280ïœÎŒãæããããã¯ååéçŽ10000ãæããã
ãªããããã§ããã èå±€ã²ã«ã¯ãããã°ã©ãã€æž¬å®ã«ãã瀺ããã
ããã«ãå¹å°ã®ç¶ç¶ããäžä»£ã«ãããŠå®å®ããã
ãå¹é€å¹å°ã«ãããŠè£œããããªã°ãã³ã®ååéã
衚ã«ç€ºããååéæž¬å®ã®åçŸæ§ã¯TLGã¯ãã
ãã°ã©ãã€ã®éºäŒçãªå¶çŽã®èгç¹ããé¡èã§ã
ãã
æ¿åºŠã®èããããæžå°ããããè³ã®ä»ã®éšã«åé
ãŸãã¯ããå€ãã®ã¬ã³ã°ãã³ãæãããšã瀺ãã
ãŠãããã¬ã³ã°ãã³ã¯è³ã®ä»ã®éšåããå°è³ãžç§»
åããªãããŸãã¯ããªãŒã©ãŒããŠã¹ã®è³ã®ä»ã®å
æã§è¥å¹²å¢å ããŠããããšã¯ããè£åè¡çºãåæ
ããŠããã ãªãŒã©ãŒããŠã¹ã®è³ã«ãããç çåŠçã¬ã³ã°ã
ã³ã¯ã现èå ã«ãããŠãèªç¥ã«ãããã¬ã³ã°ãã³
ã®åœ¹å²ã確ããªãã®ãšãããã€èªèããé©åœãªäœ
眮ã«äœçœ®ããããšãéæããããã®ãç§»åããèœ
现èãããªããã°ãªããªãæ¥è§Šãã§ããªãããšãš
é¢é£ããŠããã åæ§ã«ããŠãããŠã¹ã®ãªãŒã©ãŒã®è³ãã補ãã
ããã¬ã³ã°ãã³ã«å¯Ÿããæã¬ã³ã°ãã³ã補ãã
ããããã«ã®ã¹ãããã«ãæã¢ã¹ããã·ãã³ãã
ã³æã¢ããªã°ãã³ã®äœ¿çšæ³ãšåæ§ã«ããŠããŠã¹ã«
䜿çšãããŠããã 宿œäŸ ïŒ äººå·¥ã¬ã³çްèå¹å°çºé µã«ãããããªã°ãã³å é§
äœã®çæïŒâ äžè¬ã«ãæ» èåŠçã¯æ³šææ·±ãè¡ãªãããã ãã¹ãŠã®æº¶æ¶²ïŒããšãã°ããã³ã¯ã®å¹³è¡¡å¡©
ïŒBSSïŒãâ10æ é€åªäœãåçã®è±å ã®è¡æž ãã
ãªãã·ã³æº¶æ¶²ïŒãã䜿çšåã«æ°ŽæµŽäžãçŽ35âã§çŽ
20åãããã¯ãã以äžã®æéå¹é€ããã äžèšã®åŠãé©åœãªåªäœãçšããããšã«ããã现
èã®è «ççµç¹ããåé¢ããå€äžä»£ã«ããã€ãŠè©Šéš
管å ã§å¢æ®ãããæ» èæº¶æ¶²ãããšãã°ã12âãã
ãããŒã«ããã³ã¢ã ãã€ã«ãŸãã¯ã¯ã¬ãªãªã³æº¶æ¶²
ã§ãããããæŽæµããããŒã«ãçšããã 奜ãŸããå ·äœäŸãšããŠã人工ã¬ã³çްèïŒããªã
ã¡ãå€äžä»£ã®éã詊éšç®¡å ã§å¢æ®ãã现èïŒã
250ml容ãã©ã¹ã³ã§çè²ããã现èã墿®ããæ¶²
äœåªäœããããããæŽæµããããŒã«ã«å ¥ãããã€
ãã§çްèããã³ã¯ã®BSSãŸãã¯ä»ã®é¡äŒŒã®æº¶æ¶²ã
çšããçŽ30ç§ééãã«æŽæµãããæ¹æãé¿ããã
ãã¹ãŠã®å£ããã³è¡šé¢ãæŽæµãããå·äžã§çŽ10å
éã3000rpmã®é å¿åé¢ã«ããæº¶æ¶²ã现èããæž
æŸã«ãããåªäœãäžèšã®åŠããããŒã«ãŒã«æ³šãã
å°éã®ç·©è¡ãããã€ããŒãŒã»ãšã³ã¶ã€ã 溶液ãå
ãã现èã®æ¶åãé¿ããããã«ãã°ããæŽæµã
ãã奜ãŸããæ¹æ³ãšããŠãããªãã·ã³æº¶æ¶²
ïŒEDTAïŒïŒãïŒmlãå ããããã10ç§éæŽæµã
ããããªãã·ã³æº¶æ¶²ãæµåºãããã åãéã®æ°é®®ãªããªãã·ã³æº¶æ¶²ãå ããé¡åŸ®é¡
芳å¯ã§çްèããã€ã³ããŒå£ããåé¢ããã®ãèŠã
ãããŸã§å¹é€ãããããã«ã¯éåžžãïŒã10åéã
èŠãããé©åœãªå¢æ®åªäœãããšãã°ïŒŠâ10æ é€åª
äœ100mläžãåçã®èå ã®è¡æž ã®ïŒã10ïŒ æº¶æ¶²50
mlãå ããã 现èãšå ±ã«æ°é®®ãªåªäœ25mlãæ°ãã墿®çšãã€
ã³ããŒã«ç§»ããŠç¹æ®ããããäž¡ãã€ã³ããŒãå¹é€
æ©äžã35âã§çŽïŒæ¥é眮ããæ¬å®æœäŸã«ãããã
ããŸã§ã®æäœã«ããã人工ã¬ã³çްèå¹å°ã¯çŽïŒæ¥
ããšã«ïŒã€ã®æ°ããå¹å°ã«åå²ããããå墿®ã
ã€ã³ããŒã«ã€ããŠããã®å šæäœããã»ãïŒæ¥ã®é
éããããŠæãŸããé »åºŠã ãããè¿ãããã®ãã
ã«ããŠã詊éšç®¡å ã®çè²çŽ°èæ°ã¯ã»ãïŒæ¥ããšã«
ïŒåã«ãªãã çŽïŒæ¥éã®å¢æ®ã®åŸãããªã°ãã³ã補ãããã
ã«çްèãæœåºãããããšãã°ãäžèšå250ml墿®
çšãã€ã³ããŒå ã®å¢æ®çްèã¯ä»¥äžã®åŠãåçãã
ãã åªäœãé å¿åé¢ç®¡ã«ãã€ããå·äžã3000rpmã§
10åéé å¿åé¢ãããåªäœãæšãŠãã墿®çšãã€
ã³ããŒå ã«æ®ã€ãŠãã现èããã€ã³ããŒå£ããå¥
é¢ããŠãäžæ§ç·©è¡æº¶æ¶²ã§é å¿å颿©å ã«æŽãæµã
蟌ãã现èãäžæ§ç·©è¡æº¶æ¶²ã§ïŒåæŽããåã³ãå·
äžã3000rpmã§é å¿åé¢ããåªäœãæšãŠããç²ã
ãªã°ãã³å é§äœå«æåç»ã®æœåºã®ããã®æºåãã§
ãããŸã§äžæ§çé žå¡©ç·©è¡æ¶²10mläžã«æŽæµãã现è
ãæžæ¿ãããã 宿œäŸ ïŒ ç²ããªã°ãã³å é§äœå«æåç»ã®çæïŒâ 宿œäŸïŒã§è£œãããäžæ§ç·©è¡æ¶²äžã«æžæ¿ããæŽ
æµãã现èãã倧éšåã®èçœã®å€æ§ãé¿ãåŸãæ¡
ä»¶äžã«æ©æ¢°çã«ç²ç ããã奜ãŸããæ¹æ³ãšããŠã
æŽæµãã现èãå·äžãè¶ é³æ³¢ã§20ç§éåŠçããã
è¶ é³æ³¢åŠçåŸãçŽ°èæ®æž£ã30åéã30000rpmã§
é å¿åé¢ããäžæŸæ¶²ãåŸæåé¢ãããç·©è¡æº¶æ¶²10
mléšãçšããŠæ®çããçŽ°èæ®æž£ãæŽæµãããè¶ é³
æ³¢åŠçããã³é å¿åé¢åŠçãäžèšã®åŠãè¡ããäž
æŸæ¶²ãåãããã®æäœãå床ãããããã äžæŸæ¶²ãåããååã«èžçºãããŠå®¹éãçŽ30ml
ããçŽïŒãïŒmlã«ãããæšæ¬ãå šèçœåæã«çš
ããæ®éšããã¢ã¹ããã·ãã³å é§äœã«ã€ããŠã®å
èšå®æœäŸïŒã«èšèŒã®æ¹æ³ã«åŸã€ãŠåå¥ããã 宿œäŸ ïŒ ç²ããªã°ãã³å«æåç»ããã®ç²Ÿè£œããªã°ãã³ç
æç©ã®çæïŒâ ã¢ã¹ããã·ãã³ã«ã€ããŠã®å®æœäŸïŒã®æ¹æ³ã«ã
ã€ãŠããªã°ãã³çæç©ãããã«æ±æç©è³ªããåé¢
ããã 奜ãŸããå ·äœäŸã®TLG段éã§ã¯ãããªã°ãã³
çæç©ã¯æšæºã·ãã¯ãã ã«å¯Ÿæ¯ããŠçŽ0.91ïŒïŒ
â0.02ã®è·é¢ã«åå¥ã®æç¹ãšããŠèгå¯ãããããª
ã°ãã³ã«å¯ŸãååéçŽ10000ãäžããããã ãã®æ®µéã§è£œããããããªã°ãã³çæç©ã¯èžç
æ°Žã«å¯æº¶ã§ãããäžæ§ãŸãã¯é žæ§PHã§å¯æº¶ãã¢ã«
ã«ãªPHã§äžæº¶ã§ãããåå å 床èšçåžåããŒã¯
280ïœÎŒãæããããã¯ååéçŽ10000ãæããã
ãªããããã§ããã èå±€ã²ã«ã¯ãããã°ã©ãã€æž¬å®ã«ãã瀺ããã
ããã«ãå¹å°ã®ç¶ç¶ããäžä»£ã«ãããŠå®å®ããã
ãå¹é€å¹å°ã«ãããŠè£œããããªã°ãã³ã®ååéã
衚ã«ç€ºããååéæž¬å®ã®åçŸæ§ã¯TLGã¯ãã
ãã°ã©ãã€ã®éºäŒçãªå¶çŽã®èгç¹ããé¡èã§ã
ãã
ã衚ã
ããªã°ãã³ã®å
±æçµåã¢ããé
žã¯6NHClã§å
æ°Žåè§£ããã€ãã§å®é枬å®ã«ãã€ãŠä»¥äžã®ã¢ãã
é žã®å¹³åçµæãæããããšã瀺ãããã æ®åºã®æ°ïŒçŽïŒ ã¢ã¹ãã©ã®ã³é ž ïŒ ãã¬ãªãã³ ïŒ ã»ãªã³ ïŒ ã°ã«ã¿ãã³é ž 13 ãããªã³ ïŒ ã°ãªã·ã³ ïŒ ã¢ã©ãã³ ïŒ ããªã³ ïŒ 1/2ã·ã¹ãã€ã³ ïŒ ã¡ããªãã³ ïŒ ã€ãœãã€ã·ã³ ïŒ ãã€ã·ã³ ïŒ ãã€ãã·ã³ ïŒ ããšãã«ã¢ã©ãã³ ïŒ ãªã·ã³ ïŒ ãã¹ããžã³ ïŒ ã¢ã«ã®ãã³ ïŒ èš 89 ã¢ããé žã·ã¹ãã³é žãããããã·ãããªã³ãã
ã«ãã€ã·ã³ãã¢ã³ã¢ãã¢ãã€ãœãã¹ã¢ã·ã³ããã¹
ã¢ã·ã³ãããããã·ãªã·ã³ããªã·ããã«ãã€ã·ã³
ããã³ã¬ã³ãã¢ããããã«é žã¯æ€åºãåŸãéååš
ããªãã 宿œäŸïŒã®12åã®250mlåå¿ãã€ã³ããŒããåŸ
ãããçŽããªã°ãã³ã®åéã¯ãããªã°ãã³çŽïŒmg
ã§ããã ããªã°ãã³ãšã¢ã¹ããã·ãã³ã®ç¹ç°ã®æ§é ã¯ã
ãããã®çµæãå®è³ªçã«æ¢ç¥ã®èçœç©è³ªãšæ¯èŒã
ã培åºçãªã³ã³ããŠãŒã¿ã«ãããµãŒãã«ãã€ãŠç¢º
ãããããã ããªã°ãã³ããã³ã¢ã¹ããã·ãã³ã®ã¢ããé žå
åç©ãïŒã¢ã«ãããã®ã¢ããé žæ®åºã®ç·æ°ã®èгç¹
ããã®ãåã¢ããé žã³ã³ããã³ãã®çµ¶å¯Ÿéããã³
çžå¯Ÿéã䞊ã³ã«ååã®ååéã®èгç¹ããã®ãåã¢
ããé žã³ã³ããã³ãã®çµ¶å¯Ÿéããã³çžå¯Ÿéããäž
çã§ãèçœçµç¹ã«ã€ããŠæãåºæ±ã«ç¥ãããæ
ç®ãããªãã¡ãã·ãšãã«ã»ãã€ãªã¡ãã€ã«ã«ã»ãª
ãµãŒãã»ãã¢ãŠã³ãã€ã·ãšã³ïŒã¯ã·ã³ãã³ã»ãã€
ãŒã»ã·ãŒïŒã®ããã«å¯ŸããŠãããªãã¯ã¹ã»ã³ã³ã
ãŠãŒã¿åæã«ãã ãããäœçŸãäœåã®èçœããã³
èçœãã©ã°ã¡ã³ããšæ¯èŒãããããªãã¯ã¹åæã«
ãããŠãã¢ã¹ããã·ãã³ãŸãã¯ããªã°ãã³ã®çµç¹
ãšåäžãããã¯éåžžã«è¿äŒŒããçµç¹ããèŠåºãã
ãªãã ãšã«ããçµç¹çã«é¢ä¿ããèçœã®ã¿ããã®åã
ã®ã¢ããååç©ãšååéã«ã€ããŠæ¯èŒã®ããã«è¡š
ã«ç€ºããç¡æ°ã®å¯èœæ§ã®äžãããçµç¹ã«ããã
åçšåºŠã®é¡äŒŒæ§ã確å®ãã¹ãã³ã³ããŠãŒã¿ã®ãã
ã°ã©ã ãçµãã ããã®ããã«ããŠãããšãã°ãã
ã倧ããªãŸãã¯ããå°ããååéã®èçœããŸãã¯
85ããå°ãªãããããã¯95ããå€ãæ®åºãæãã
èçœããŸãã¯12ããå°ãªãããããã¯15ããå€ã
ã°ã«ã¿ãã³é žé žåºãæããèçœããŸãã¯ïŒããå°
ãªãããããã¯11ããå€ãã¢ã¹ãã©ã®ã³æ®åºãæ
ããèçœãªã©ã¯å å«ããã20ã®ã¢ããé žãšåèŽã
ãªãã
æ°Žåè§£ããã€ãã§å®é枬å®ã«ãã€ãŠä»¥äžã®ã¢ãã
é žã®å¹³åçµæãæããããšã瀺ãããã æ®åºã®æ°ïŒçŽïŒ ã¢ã¹ãã©ã®ã³é ž ïŒ ãã¬ãªãã³ ïŒ ã»ãªã³ ïŒ ã°ã«ã¿ãã³é ž 13 ãããªã³ ïŒ ã°ãªã·ã³ ïŒ ã¢ã©ãã³ ïŒ ããªã³ ïŒ 1/2ã·ã¹ãã€ã³ ïŒ ã¡ããªãã³ ïŒ ã€ãœãã€ã·ã³ ïŒ ãã€ã·ã³ ïŒ ãã€ãã·ã³ ïŒ ããšãã«ã¢ã©ãã³ ïŒ ãªã·ã³ ïŒ ãã¹ããžã³ ïŒ ã¢ã«ã®ãã³ ïŒ èš 89 ã¢ããé žã·ã¹ãã³é žãããããã·ãããªã³ãã
ã«ãã€ã·ã³ãã¢ã³ã¢ãã¢ãã€ãœãã¹ã¢ã·ã³ããã¹
ã¢ã·ã³ãããããã·ãªã·ã³ããªã·ããã«ãã€ã·ã³
ããã³ã¬ã³ãã¢ããããã«é žã¯æ€åºãåŸãéååš
ããªãã 宿œäŸïŒã®12åã®250mlåå¿ãã€ã³ããŒããåŸ
ãããçŽããªã°ãã³ã®åéã¯ãããªã°ãã³çŽïŒmg
ã§ããã ããªã°ãã³ãšã¢ã¹ããã·ãã³ã®ç¹ç°ã®æ§é ã¯ã
ãããã®çµæãå®è³ªçã«æ¢ç¥ã®èçœç©è³ªãšæ¯èŒã
ã培åºçãªã³ã³ããŠãŒã¿ã«ãããµãŒãã«ãã€ãŠç¢º
ãããããã ããªã°ãã³ããã³ã¢ã¹ããã·ãã³ã®ã¢ããé žå
åç©ãïŒã¢ã«ãããã®ã¢ããé žæ®åºã®ç·æ°ã®èгç¹
ããã®ãåã¢ããé žã³ã³ããã³ãã®çµ¶å¯Ÿéããã³
çžå¯Ÿéã䞊ã³ã«ååã®ååéã®èгç¹ããã®ãåã¢
ããé žã³ã³ããã³ãã®çµ¶å¯Ÿéããã³çžå¯Ÿéããäž
çã§ãèçœçµç¹ã«ã€ããŠæãåºæ±ã«ç¥ãããæ
ç®ãããªãã¡ãã·ãšãã«ã»ãã€ãªã¡ãã€ã«ã«ã»ãª
ãµãŒãã»ãã¢ãŠã³ãã€ã·ãšã³ïŒã¯ã·ã³ãã³ã»ãã€
ãŒã»ã·ãŒïŒã®ããã«å¯ŸããŠãããªãã¯ã¹ã»ã³ã³ã
ãŠãŒã¿åæã«ãã ãããäœçŸãäœåã®èçœããã³
èçœãã©ã°ã¡ã³ããšæ¯èŒãããããªãã¯ã¹åæã«
ãããŠãã¢ã¹ããã·ãã³ãŸãã¯ããªã°ãã³ã®çµç¹
ãšåäžãããã¯éåžžã«è¿äŒŒããçµç¹ããèŠåºãã
ãªãã ãšã«ããçµç¹çã«é¢ä¿ããèçœã®ã¿ããã®åã
ã®ã¢ããååç©ãšååéã«ã€ããŠæ¯èŒã®ããã«è¡š
ã«ç€ºããç¡æ°ã®å¯èœæ§ã®äžãããçµç¹ã«ããã
åçšåºŠã®é¡äŒŒæ§ã確å®ãã¹ãã³ã³ããŠãŒã¿ã®ãã
ã°ã©ã ãçµãã ããã®ããã«ããŠãããšãã°ãã
ã倧ããªãŸãã¯ããå°ããååéã®èçœããŸãã¯
85ããå°ãªãããããã¯95ããå€ãæ®åºãæãã
èçœããŸãã¯12ããå°ãªãããããã¯15ããå€ã
ã°ã«ã¿ãã³é žé žåºãæããèçœããŸãã¯ïŒããå°
ãªãããããã¯11ããå€ãã¢ã¹ãã©ã®ã³æ®åºãæ
ããèçœãªã©ã¯å å«ããã20ã®ã¢ããé žãšåèŽã
ãªãã
ã衚ã
ã衚ã
äžèšã®ããšãæçŽã¯é©åãã¹ã22åã®å€æ°ãæ
ãããããç©è³ªã¯ïŒåãïŒåãããã¯ïŒåã®å€æ°
ã«é¢ããŠé©åãããã22åã®å€æ°ãã¹ãŠã«é¢ããŠ
é©åãããã®ã¯ãªããã®ãšèŠããããäºå®ã14å
ïŒãã®å ãïŒåã®å€æ°ã«ãããŠå·®ç°ãèªãããïŒã
è¶ãã倿°ã«é¢ããŠããé©åãããã®ã¯ãªãã ããæ ãæãå¯ã«é©åãããã®ã¯ããã¯ããŒã
b5ïŒäººéã®ïŒã§ããã衚äžã«èŠãããããã«ã
ãã®ã¢ã©ãã³ãã¢ã«ã®ãã³ãã¢ã¹ãã©ã®ã³ãã¢ã¹
ãã©ã®ã³é žãã·ã¹ãã€ã³ãã°ã«ã¿ãã³ããã¹ããž
ã³ãã¡ããªãã³ãããã·ã³ãããªãããã¢ã³æ®åº
ã¯ãã¹ãŠã¢ã¹ããã·ãã³ããã³ããªã°ãã³ãšã¯è
ããå·®ç°ãããããšãèå¥ããããšãã§ãããã·
ãã¯ãã b5ã¯ãã¹ããžã³ååïŒåãå«ãã®ã«å¯Ÿã
ãŠãã¢ã¹ããã·ãã³ããã³ããªã°ãã³ã¯ãã®åå
ïŒåã®ã¿å«ãã®ã§ãäž¡è ãåäžã®ååŠæ§é ãæã
ãå¯èœæ§ã¯ãªããã¢ã¹ããã·ãã³ããã³ããªã°ã
ã³ã®çµæã«é¢ããæãç°ç¶ãªäºæã¯ã°ã«ã¿ãã³é ž
æ¿åºŠãé«ãç¹ã«ããã89åäžãæ®åºïŒãïŒåã®ã¿
ãèŠåºãåŸãããã«æåŸ ãããã äžèšã«æ¬¡ãã§å¯æ¥ã«é©åãããã®ã¯ãã€ã«ãš
ãã»ã°ã©ãŠã«ïŒLeucaene glaucaïŒããã³ã¢ã«ã
ã¢ã«ãã¢äžã®ããšãããã·ã³é¡ã§ããããããã
ã¯ããããã¢ããé žïŒåããã³ïŒåãæããããš
ã«ãããŠç°ãªããäžã€ãã®äž¡è ã¯ããããæ®åº96
åããã³97åãæããç¹ã§ç°ãªãããã®ä»ã®é©å
ãããã®ãšããŠå€§è žèïŒïŒ¥ãcoliïŒ26ã®ã¢ã·ã«æ
äœèçœãæããããããããã¯æ®åºæ°ãã¢ããé ž
11åã«ãããŠããªã°ãã³ããã³ã¢ã¹ããã·ãã³ãš
èããç°ãªãããŸãããã¯æ®åº77åã®ã¿ãæãã
ç¹ã§ç°ãªãã ä»ã®è³èçœïŒçãè±ã®ãã€ããã€ã·ã³ããã³ãŽ
ãããããã³æŸåºãã«ã¢ã³ïŒãèšé²ããïŒè¡šå
ç §ïŒãããã®ãã®ã®ã³ã³ããŠãŒã¿ã«èšæ¶ãããæ°
10äžçš®ã®èçœãã©ã°ã¡ã³ããããããããéåžžã«
é©åã®æªããã®ã§ããã åŒåžèçœã¯ãã®å ã®ãŸãŸã®ç¶æ ã«ãããŠéå±ã
ãã³ïŒãŸãã¯ãã æåã嫿ããããšãã§ãã
ããåé¢ãããç¶æ ã®èçœãã©ã°ã¡ã³ããããšã
ã°ã¢ãã·ãã¯ãã b5ã¯éå±ããã³ãã æåã®ãã¥
ããã嫿ããªããã¢ã¹ããã·ãã³ããã³ããªã°
ãã³ã®åŸ®éåæçµæã«ããã°ãéãç¡«é»ããªã³ã
ãã°ãã·ãŠã ã嫿ããããšãªãïŒ0.01ïŒ ä»¥äžïŒã
280ïœÎŒã«å žåçåžåã¹ãã¯ãã«ç¹æ§ãæããã
ã¢ããããã€ã³ãšå ±ã«ãã ç©è³ªãæ§æããããã
ã®åžåã¹ãã¯ãã«ã枬å®ããçµæã400ããã³450
ïœÎŒã®éã«å žåçåžåãèªããããã ã¢ã¹ããã·ãã³ããã³ããªã°ãã³ã¯ä»ã®ãã¹ãŠ
ã®èçœããã³èçœãã©ã°ã¡ã³ããšç°ãªããæ§é ç
ã«ç°ãªããã®ã§ããã«ãæããããããã®æ§é ã¯
åŒåžèçœã®ãããšå¯æ¥ãªé¢é£æ§ãæãããšããã
ãšã¯æ³šç®ãã¹ãããšã§ãã€ãŠãå€åéåžžã«éèŠã§
ãããçºçéºäŒåŠç芳ç¹ããã³ãã®ååŠæ§é ã«ã
ããæ©èœç芳ç¹ã®åæ¹ãããå€ãã®éèŠãªé¢é£æ§
ãåŒåºãããšãã§ãããšããããšã¯ãã®æè¡åé
ã«ãããŠããç¥ãããŠãããã¢ã¹ããã·ãã³ãã
ã³ããªã°ãã³ãæ°ããèçœç©è³ªã§ãã€ãŠãã®æ§é
çç䟡ç©ããã®äœçœ®ã§åŒåžäœçšã瀺ããåŸã€ãŠã
ããã®èçœãä»ã«éäœçšã瀺ãã®ã§ãããããç
ã®å€§åé¡ã®ïŒã€ïŒããªãã¡è²§æ¬²äžã€æ¥éã«å¢æ®ã
ãæªæ§çްèã«å¯Ÿãããšãã«ã®ãŒé¢ä¿ãããã«ããŠ
æºè¶³ãªãã®ãšããããšããåé¡ïŒã解決ãããã
ã®å æãäžããããšãšãªã€ãã ãããçºèŠã®çè«çéèŠæ§ã¯ãšããããšããŠã
ããæªæ§ãªçްèäžã«ããªã°ãã³ã®å²åãå¢å ãã
ããåèšããŒã¿ããã³æããªã°ãã³ç©è³ªã¯ç现è
ã®ããªã°ãã³æ§ååŠç©è³ªã«çµåããŠããã°ããã§
ãªããããçµåããæããªã°ãã³ããŸãç现èã«
察ããŠæ¯æ§ãæããããšã瀺ããåŸèšããŒã¿ã¯å ±
ã«éèŠãªæçŸ©ãæãããæ¬çºæã«ãããæããªã°
ãã³çæç©ã¯ç现èäžã§åå¥çã«ãã®äžã®ãã å
åç©ã«çµåããŠããããããããã®ããªã°ãã³æ§
ååç©ãåŒåžèçœã§ãããåŸã€ãŠãã®ç³»å ã«åŒåž
æ©èœãæããç©è³ªã嫿ããã®ã§ããã°ããã®ç
现èãæ»æ» ãããåŸãããšã¯å®¹æã«çè§£ãåŸããš
ããã§ããã æããªã°ãã³é¡ããã³ä»ã®é¡äŒŒã®ååŠçéäœçš
ç©è³ªã®æ²»çç广ã«å¯Ÿããå¯èœæ§ã¯ããªã°ãã³ã
ãã³ã¢ã¹ããã·ãã³ã«é¢ããæ§é çç¥èŠããã³ã
ãªã°ãã³éã®æªæ§åºŠãšã®éã«æ¢ã«èšŒæãããé¢ä¿
ã«ããèããå¢å€§ãããã 宿œäŸ 5A çºé µã®éçšã«ãããŠãã®å®¹éããã³è¡šé¢ç©ãå¢
å ããããããšã«ããåéãæªæ§ã®åºŠåããã³
ããªã°ãã³ã®å²åã®å¢å€§ïŒ 250mlãã©ã¹ã³ã®ä»£ããã«1000mlãã©ã¹ã³ãçš
ãã詊è¬ã®éã¯ãã¹ãŠïŒåéãå¢å ãã宿œäŸïŒ
ãïŒãšåæ§ã®æäœãè¡ã€ãã æªæ§çްèã®å¢æ®ã®ããã®ã¹ããŒã¹ã250mlãã
1000mlã«å¢å ããããšã«ãããæ¥çš®ããïŒæ¥éçº
è²ãããåŸã®ããªã°ãã³çæç©ã®åéã¯ã»ãšãã©
ïŒåã«å¢å ããããã©ã¹ã³ã®å€§ããå¥ã«ããã®ç·
èçœåéïŒmgïŒããã³çæããããªã°ãã³ãé£ç¶
çäžä»£ã®çºé µç©è³ªã®ç·èçœéã«å¯ŸããïŒ ãšããŠè¡š
ãããããã衚ã«ç€ºãã250mlãã©ã¹ã³ãçšã
ããšããçæããç·èçœéã®å¹³åå€ã¯17.5mgã
1000mlãã©ã¹ã³ãçšãããšããçæããç·èçœé
ã®å¹³åå€ã¯40.4mgã§ãã€ãã
ãããããç©è³ªã¯ïŒåãïŒåãããã¯ïŒåã®å€æ°
ã«é¢ããŠé©åãããã22åã®å€æ°ãã¹ãŠã«é¢ããŠ
é©åãããã®ã¯ãªããã®ãšèŠããããäºå®ã14å
ïŒãã®å ãïŒåã®å€æ°ã«ãããŠå·®ç°ãèªãããïŒã
è¶ãã倿°ã«é¢ããŠããé©åãããã®ã¯ãªãã ããæ ãæãå¯ã«é©åãããã®ã¯ããã¯ããŒã
b5ïŒäººéã®ïŒã§ããã衚äžã«èŠãããããã«ã
ãã®ã¢ã©ãã³ãã¢ã«ã®ãã³ãã¢ã¹ãã©ã®ã³ãã¢ã¹
ãã©ã®ã³é žãã·ã¹ãã€ã³ãã°ã«ã¿ãã³ããã¹ããž
ã³ãã¡ããªãã³ãããã·ã³ãããªãããã¢ã³æ®åº
ã¯ãã¹ãŠã¢ã¹ããã·ãã³ããã³ããªã°ãã³ãšã¯è
ããå·®ç°ãããããšãèå¥ããããšãã§ãããã·
ãã¯ãã b5ã¯ãã¹ããžã³ååïŒåãå«ãã®ã«å¯Ÿã
ãŠãã¢ã¹ããã·ãã³ããã³ããªã°ãã³ã¯ãã®åå
ïŒåã®ã¿å«ãã®ã§ãäž¡è ãåäžã®ååŠæ§é ãæã
ãå¯èœæ§ã¯ãªããã¢ã¹ããã·ãã³ããã³ããªã°ã
ã³ã®çµæã«é¢ããæãç°ç¶ãªäºæã¯ã°ã«ã¿ãã³é ž
æ¿åºŠãé«ãç¹ã«ããã89åäžãæ®åºïŒãïŒåã®ã¿
ãèŠåºãåŸãããã«æåŸ ãããã äžèšã«æ¬¡ãã§å¯æ¥ã«é©åãããã®ã¯ãã€ã«ãš
ãã»ã°ã©ãŠã«ïŒLeucaene glaucaïŒããã³ã¢ã«ã
ã¢ã«ãã¢äžã®ããšãããã·ã³é¡ã§ããããããã
ã¯ããããã¢ããé žïŒåããã³ïŒåãæããããš
ã«ãããŠç°ãªããäžã€ãã®äž¡è ã¯ããããæ®åº96
åããã³97åãæããç¹ã§ç°ãªãããã®ä»ã®é©å
ãããã®ãšããŠå€§è žèïŒïŒ¥ãcoliïŒ26ã®ã¢ã·ã«æ
äœèçœãæããããããããã¯æ®åºæ°ãã¢ããé ž
11åã«ãããŠããªã°ãã³ããã³ã¢ã¹ããã·ãã³ãš
èããç°ãªãããŸãããã¯æ®åº77åã®ã¿ãæãã
ç¹ã§ç°ãªãã ä»ã®è³èçœïŒçãè±ã®ãã€ããã€ã·ã³ããã³ãŽ
ãããããã³æŸåºãã«ã¢ã³ïŒãèšé²ããïŒè¡šå
ç §ïŒãããã®ãã®ã®ã³ã³ããŠãŒã¿ã«èšæ¶ãããæ°
10äžçš®ã®èçœãã©ã°ã¡ã³ããããããããéåžžã«
é©åã®æªããã®ã§ããã åŒåžèçœã¯ãã®å ã®ãŸãŸã®ç¶æ ã«ãããŠéå±ã
ãã³ïŒãŸãã¯ãã æåã嫿ããããšãã§ãã
ããåé¢ãããç¶æ ã®èçœãã©ã°ã¡ã³ããããšã
ã°ã¢ãã·ãã¯ãã b5ã¯éå±ããã³ãã æåã®ãã¥
ããã嫿ããªããã¢ã¹ããã·ãã³ããã³ããªã°
ãã³ã®åŸ®éåæçµæã«ããã°ãéãç¡«é»ããªã³ã
ãã°ãã·ãŠã ã嫿ããããšãªãïŒ0.01ïŒ ä»¥äžïŒã
280ïœÎŒã«å žåçåžåã¹ãã¯ãã«ç¹æ§ãæããã
ã¢ããããã€ã³ãšå ±ã«ãã ç©è³ªãæ§æããããã
ã®åžåã¹ãã¯ãã«ã枬å®ããçµæã400ããã³450
ïœÎŒã®éã«å žåçåžåãèªããããã ã¢ã¹ããã·ãã³ããã³ããªã°ãã³ã¯ä»ã®ãã¹ãŠ
ã®èçœããã³èçœãã©ã°ã¡ã³ããšç°ãªããæ§é ç
ã«ç°ãªããã®ã§ããã«ãæããããããã®æ§é ã¯
åŒåžèçœã®ãããšå¯æ¥ãªé¢é£æ§ãæãããšããã
ãšã¯æ³šç®ãã¹ãããšã§ãã€ãŠãå€åéåžžã«éèŠã§
ãããçºçéºäŒåŠç芳ç¹ããã³ãã®ååŠæ§é ã«ã
ããæ©èœç芳ç¹ã®åæ¹ãããå€ãã®éèŠãªé¢é£æ§
ãåŒåºãããšãã§ãããšããããšã¯ãã®æè¡åé
ã«ãããŠããç¥ãããŠãããã¢ã¹ããã·ãã³ãã
ã³ããªã°ãã³ãæ°ããèçœç©è³ªã§ãã€ãŠãã®æ§é
çç䟡ç©ããã®äœçœ®ã§åŒåžäœçšã瀺ããåŸã€ãŠã
ããã®èçœãä»ã«éäœçšã瀺ãã®ã§ãããããç
ã®å€§åé¡ã®ïŒã€ïŒããªãã¡è²§æ¬²äžã€æ¥éã«å¢æ®ã
ãæªæ§çްèã«å¯Ÿãããšãã«ã®ãŒé¢ä¿ãããã«ããŠ
æºè¶³ãªãã®ãšããããšããåé¡ïŒã解決ãããã
ã®å æãäžããããšãšãªã€ãã ãããçºèŠã®çè«çéèŠæ§ã¯ãšããããšããŠã
ããæªæ§ãªçްèäžã«ããªã°ãã³ã®å²åãå¢å ãã
ããåèšããŒã¿ããã³æããªã°ãã³ç©è³ªã¯ç现è
ã®ããªã°ãã³æ§ååŠç©è³ªã«çµåããŠããã°ããã§
ãªããããçµåããæããªã°ãã³ããŸãç现èã«
察ããŠæ¯æ§ãæããããšã瀺ããåŸèšããŒã¿ã¯å ±
ã«éèŠãªæçŸ©ãæãããæ¬çºæã«ãããæããªã°
ãã³çæç©ã¯ç现èäžã§åå¥çã«ãã®äžã®ãã å
åç©ã«çµåããŠããããããããã®ããªã°ãã³æ§
ååç©ãåŒåžèçœã§ãããåŸã€ãŠãã®ç³»å ã«åŒåž
æ©èœãæããç©è³ªã嫿ããã®ã§ããã°ããã®ç
现èãæ»æ» ãããåŸãããšã¯å®¹æã«çè§£ãåŸããš
ããã§ããã æããªã°ãã³é¡ããã³ä»ã®é¡äŒŒã®ååŠçéäœçš
ç©è³ªã®æ²»çç广ã«å¯Ÿããå¯èœæ§ã¯ããªã°ãã³ã
ãã³ã¢ã¹ããã·ãã³ã«é¢ããæ§é çç¥èŠããã³ã
ãªã°ãã³éã®æªæ§åºŠãšã®éã«æ¢ã«èšŒæãããé¢ä¿
ã«ããèããå¢å€§ãããã 宿œäŸ 5A çºé µã®éçšã«ãããŠãã®å®¹éããã³è¡šé¢ç©ãå¢
å ããããããšã«ããåéãæªæ§ã®åºŠåããã³
ããªã°ãã³ã®å²åã®å¢å€§ïŒ 250mlãã©ã¹ã³ã®ä»£ããã«1000mlãã©ã¹ã³ãçš
ãã詊è¬ã®éã¯ãã¹ãŠïŒåéãå¢å ãã宿œäŸïŒ
ãïŒãšåæ§ã®æäœãè¡ã€ãã æªæ§çްèã®å¢æ®ã®ããã®ã¹ããŒã¹ã250mlãã
1000mlã«å¢å ããããšã«ãããæ¥çš®ããïŒæ¥éçº
è²ãããåŸã®ããªã°ãã³çæç©ã®åéã¯ã»ãšãã©
ïŒåã«å¢å ããããã©ã¹ã³ã®å€§ããå¥ã«ããã®ç·
èçœåéïŒmgïŒããã³çæããããªã°ãã³ãé£ç¶
çäžä»£ã®çºé µç©è³ªã®ç·èçœéã«å¯ŸããïŒ ãšããŠè¡š
ãããããã衚ã«ç€ºãã250mlãã©ã¹ã³ãçšã
ããšããçæããç·èçœéã®å¹³åå€ã¯17.5mgã
1000mlãã©ã¹ã³ãçšãããšããçæããç·èçœé
ã®å¹³åå€ã¯40.4mgã§ãã€ãã
ã衚ã
ã衚ã
é©ãã¹ãããšã«ã现è墿®ã®ããã®ã¹ããŒã¹ãš
衚é¢ç©ãå¢å ããããããšã«ãããïŒæ¥çºè²æé
åœããæªæ§çްè墿®éïŒæªæ§ã®åºŠåïŒãå¢å€§ãã
ã«åŸã€ãŠç·èçœã®ïŒ ãšããŠç€ºãããçæããªã°ã
ã³éããŸãææã«å¢å€§ãããïŒæ¥ã®çºè²æéäžã«
ç·èçœéã«å¯Ÿããããªã°ãã³ã®çŸåçã¯250mlã
ã©ã¹ã³äœ¿çšæã«ãããå¹³åå€10.7ïŒ ããã1000ml
ãã©ã¹ã³äœ¿çšæã«ãããå¹³åå€28.3ïŒ ã«å¢å ã
ãã çæããããªã°ãã³ã®å²åãšæªæ§ã®åºŠåïŒããª
ãã¡ïŒæ¥é詊éšç®¡å ã«ãããæªæ§çްè墿®éã«å¯Ÿ
ããçæèçœéã®é¢æ°ïŒãšã®é¢ä¿ã第ïŒå³ã«ç€º
ãã 第ïŒå³ã¯ãã©ã¹ã³ã®å€§ãããå¢å ããã®ã§ããª
ã°ãã³çæç©ã®æ¯çã¯çŽïŒåã«å¢å€§ããããšã瀺
ããŠããããŸã第ïŒå³ã¯ããªã°ãã³ãšæªæ§ã®åºŠå
ãšã®é¢ä¿ã瀺ããã®ã§ãããäžéç·ã¯çæ³ççŽç·
é¢ä¿ã瀺ãç·ã§ããããã®å®èšŒçé¢ä¿ã¯ãã®çްè
ã®å¢æ®ãïŒç çåŠçã«ïŒãã黿¢ãåŸãªããã®ãš
ãªãã«åŸã€ãŠããªã°ãã³ã®ååšæ¯çãå¢å€§ããã
ã®ã§ããããéèŠãªé¢ä¿ã§ãããéåžžã®ã¬ã³ã°ã
ã³ã®æ©èœããèªäœã¯ãåèšã®ããšã现èã«æ¥è§Šã
ãŠãã®å¢æ®ãæå¶ããããšã«é¢é£æ§ãæãããæª
æ§çްèã®å¢æ®ãããç çã§ããã°ãæ¥è§Šã«ããæ
æ§å¹æã¯æžå°ããããªã°ãã³ãããåªå çãªèçœ
ãšãªã€ãŠå¢å ããã 宿œäŸ5Aã«ãããŠã¯ã倧ãªãçºè²å®¹åšäžã§çº
è²ããã人工çç现èå¹é€ç©ã¯äºæã«åããŠçæ
ããªã°ãã³ã®å Žåãããªãã¡ç·èçœéäžã®ããªã°
ãã³ã®çŸåçãå¢å€§ããããšãäŸèšŒãããã®ã§ã
ãããã®çºæã«ãããŠçšãããããããªå€§å®¹éã®
çºè²å®¹åšã¯å®¹åšå®¹éã®å®æœäŸïŒã«ãã现èå ¥å¹å°
ç·å®¹éã«å¯Ÿããæ¯ãçŽïŒïŒïŒïŒããšãã°ãã®ç¯å²
ã¯ïŒïŒïŒãªãã10ïŒïŒïŒãã倧ãªã容åšã§ããã
ãšãæå³ããã宿œäŸ5Aã«ãããŠã¯ãã®æ¯ã¯çŽ
ïŒïŒïŒã§ãã€ãã 宿œäŸ ïŒ ã¬ã³ã°ãã³é¡ããã¿ãŒã²ãã詊è¬ã®è£œé ïŒâ åèšå®æœäŸïŒã§è£œããããã¢ã¹ããã·ãã³ãŸã
ã¯å®æœäŸïŒã§è£œããããããªã°ãã³ãæ äœãšæ··å
ããŠã¿ãŒã²ãã詊è¬ã補ããã 奜ãŸãã宿œæ æ§ã«ãããŠãã¢ã¹ããã·ãã³ãŸ
ãã¯ããªã°ãã³ã0.15Mãªã³é žäºæ°ŽçŽ ãããªãŠã
âã¯ãšã³é žç·©è¡æ¶²ïŒPH4.0ïŒã«æº¶è§£ãããããã¢
ã¢ã»ãã«æš¹èãããšãã°ã»ã«ããŒã¹ïŒïœåœãèçŽ
1.0ã1.5ããªã¢ã«ãæããããã¢ã¢ã»ãã«ã»ã«ã
ãŒã¹ïŒBACïŒã0.15Mãªã³é žäºæ°ŽçŽ ãããªãŠã
ç·©è¡æ¶²ïŒPH7.2ïŒäžã§è£œããå·æã«è²¯ãããPH7.2
ã®ç·©è¡æº¶æ¶²ã泚ããäžèš0.15Mãªã³é žäºæ°ŽçŽ ãã
ãªãŠã âã¯ãšã³é žç·©è¡æ¶²ïŒPH4.0ïŒãå ããããš
ã«ãããåŸãããç·©è¡æ¶²ã®PHãïŒã«èª¿ç¯ãããã¢
ã¹ããã·ãã³ãŸãã¯ããªã°ãã³æº¶æ¶²ãšBACã®æº¶
æ¶²ïŒBACã®ã¬ã³ã°ãã³ã«å¯Ÿããæ¯10ïŒïŒïŒã宀
æž©ã§30æéæ¹æããåŸãé å¿åé¢ããã ã¬ã³ã°ãã³ã«çµåãããããã«çšããBACäž
ã®ãã¹ãŠã®ãµã€ãã¯çµåã«é¢äžããŠããããšã奜
ãŸãããããã¯æ¬¡ã®ããã«ããŠéæããããçŽäž
ã«èšèŒããæ®µéã«ãããŠåŸãããäžæŸæ¶²ãåçµä¹Ÿ
ç¥ããBACã«çµåããŠããªãã¢ã¹ããã·ãã³ãŸ
ãã¯ããªã°ãã³ã®éãç¥ãããã«èçœå«éãæž¬å®
ãããè€åBACâã¢ã¹ããã·ãã³ïŒãŸãã¯BAC
âããªã°ãã³ïŒãå床0.1Mçé žæ°ŽçŽ å¡©ç·©è¡æ¶²
ïŒPH8.9ïŒã«æžæ¿ããïŒâã§24æéæ¹æããŠBAC
ããã³ã¢ã¹ããã·ãã³ãŸãã¯ããªã°ãã³ã®éã®å
åŠçµåã圢æãããã24æéåŸããã®æžæ¿æ¶²ãé
å¿åé¢ããäžæŸæ¶²ã®èçœãåæãããè€åBAC
âã¢ã¹ããã·ãã³ãŸãã¯BACâããªã°ãã³ãæŽ
ã«0.05Mã¢ãããšã¿ããŒã«â0.1Mçé žæ°ŽçŽ å¡©ç·©
è¡æ¶²ïŒPH8.9ïŒäžã«åæžæ¿ããŠæªåå¿èçŽ ãéå¡
ããããã®æžæ¿æ¶²ãé å¿åé¢ããã¢ãããšã¿ããŒ
ã«ãååšãããããã®äžæŸæ¶²ã¯ãããåæã«ä»ã
ãããšãªããã®ãŸãŸä¿æããããããé å¿åé¢
ããã¹ãã¯ããããã¡ãŒã¿ã§266ïœÎŒã«åžåã芳
枬ãããªããªããŸã§ãã®åæžæ¿æ¶²ã0.15Må¡©åã
ããªãŠã ã§ïŒåæŽæµããããã®BACâã¢ã¹ãã
ã·ãã³ãŸãã¯BACâããªã°ãã³è€åäœã8Må°¿çŽ
äžã38âã§ïŒæéæ¹æããé å¿åé¢åŸãæŽæ¶²äž
ã«ã266ïœÎŒã®åžåãçŸãããªããªããŸã§ïŒéåžž
ïŒåïŒã8Må°¿çŽ ã§æŽæµããããã®è€åäœã0.15M
å¡©åãããªãŠã ã§ïŒåæŽæµããŠå°¿çŽ ãé€å»ããã
ãã®è€åäœã0.25Mé ¢é žäžã37âã§ïŒæéæ¹æ
ãããã®å®å®æ§ã蚌æãããé å¿åé¢ããåŸãã
ãäžæŸæ¶²ã¯å¿ ç¶çã«266ïœÎŒã«ãããåžåãååš
ããªããããæ ããã®ååŠçè€åäœïŒBACâã¢
ã¹ããã·ãã³ãŸãã¯BACâããªã°ãã³ã¯å®å®ã§
ãã€ãŠäžèšã®ããšãæ¹æ³ã«ããã詊è¬ãšããŠçšã
ãããšãã§ããããã®å®å®ãªè©Šè¬åã«ãããŠãã
ããåææ³ã«ããåŸãããé¯äœãšåŒã¶ããšãã§
ãããã®ç©ççããã³ååŠçæ§è³ªã¯æœåšçåå¿ç¶
æ ã«ãããŠè¡æž æåãšããŠçè§£ãããæãå®å®çް
èâã¢ã¹ããã·ãã³ãŸãã¯ããªã°ãã³ã®çµååé§
äœãšèŠå£ãããšãã§ãããä¿åã®ããã«ã¯ãã®ã¿
ãŒã²ãã詊è¬ãé å¿åé¢ãã0.15Mãªã³é žäºæ°ŽçŽ
ãããªãŠã ç·©è¡æ¶²ïŒPH7.2ïŒã§äžæ§ã«ãªããŸã§æŽ
ãã ã¿ãŒã²ãã詊è¬ã¯ã»ã«ããŒãºä»¥å€ã®é äœåãæ
ããããã¢ã¢ã»ãã«æ äœãããšãã°ããã¢ã¢ã»ã
ã«åæš¹èãããã¯çŽããã§ããã䜿çšããããš
ãã§ããã 宿œäŸ ïŒ ã¢ã¹ããã·ãã³ãããªã°ãã³ããã³ã¿ãŒã²ãã
ã«å¯Ÿããæè¡æž ã®è£œé ïŒâ ã¢ã¹ããã·ãã³ãããªã°ãã³ãŸãã¯ã¿ãŒã²ãã
詊è¬ã«å¯Ÿããæè¡æž ã¯åºä¹³åç©ã«ãããããã詊
è¬ã«å¯Ÿããæäœåå¿ãèªçºããããšã«ãã補ãã
ããããã®ç®çã®ããã«æºè¶³ãªæ¹æ³ã以äžèª¬æã
ãã ã¬ã³ã°ãã³ïŒã¢ã¹ããã·ãã³ããã³ããªã°ã
ã³ïŒïŒmgãæšæºããã€ã³ãäœè¬ãšå ±ã«çœè²éå®¶å
ã®è¶³ã®èè¶Ÿã«æ³šå°ããïŒé±éåŸãæŽã«10æ¥åŸãèŠ
ããã°æŽã«ïŒé±éåŸè ¹è å ã«åæ§ã®æ³šå°ãè¡ãã
æåã®æ³šå°ããïŒé±éãªãã10æ¥åŸãæ©ãããã
ã®å®¶å ã®è¡æž äžã«ç¹æ®ãªæäœãæ€åºããããšãã§
ãããã¿ãŒã²ããæåãåŸããããã¢ã¹ããã·ã
ã³ãŸãã¯ããªã°ãã³ïŒmgïŒããªãªã³âããŠãªã€è
çœæ€åºæ³ã«ããæž¬å®ïŒã嫿ããã¿ãŒã²ããéã
泚å°ããæäœãäžèšåæ§ã®æ¹æ³ã«ããè¡ããã¢ã¹
ããã·ãã³ã«å¯Ÿããç¹æ®æäœãæã¢ã¹ããã·ãã³
ïŒAntiâAstrocytinïŒãšåŒã¶ãããªã°ãã³ã«å¯Ÿã
ãç¹æ®æäœãæããªã°ãã³ïŒAntiâMaligninïŒ
ãšåŒã¶ãåæ§ã«ã¿ãŒã²ãã詊è¬ã«å¯Ÿããç¹æ®æäœ
ãæã¿ãŒã²ããïŒAntiâTargetïŒãšåŒã¶ã æåâæäœåå¿ã®ããã®æšæºçãªãŒã¯ã¿ãŒãã
ã€ïŒOuchterlonyïŒã²ã«æ¡æ£è©Šéšæ³ã«ããããã®
ç¹æ®æåã«ããåŸãããç¹æ®æäœã¯ãã®æåãšã®
åå¿ã«ãããåäžéæãªçŽç·é¢ä¿ã瀺ãããšãæ
ããã§ããã è¡æž äžã«ãããç¹æ®æäœã®ååšã¯æåâæäœå
å¿ã®ããã®æ²éçŽ æšæºå®éè©Šéšæ³ã«ãã€ãŠã詊éš
ããããšãã§ããããã®è©Šéšã«ããè¯å¥œãªæ²éçŽ
å®éæ²ç·ãåŸãããšãã§ããããããç¹æ®æäœã®
mgæ°ãèšç®ããããšãã§ããã æŽã«è¡æž äžã«ãããç¹æ®æäœã®ååšã¯ç¹æ®æ
äœïŒæã¢ã¹ããã·ãã³ãåèšã®ããšãããã¢ã¢ã»
ãã«âã»ã«ããŒã¹âã¢ã¹ããã·ãã³ïŒBACâã¢
ã¹ããã·ãã³ïŒäžã«åžåãããããšã«ãããã®èšŒ
æãåŸãããšãã§ãããããªãã¡ãç¹æ®æã¢ã¹ã
ãã·ãã³ã嫿ããæè¡æž ãšBACâã¢ã¹ããã·
ãã³ãåå¿ãããããšãã§ãããè¡æž ãBACâ
ã¢ã¹ããã·ãã³äžã«éããšããã¢ã¹ããã·ãã³ã«
察ããç¹æ®æäœã®ã¿ããã®ç¹æ®æåã¢ã¹ããã·ã
ã³ã«çµåãããã¢ã¹ããã·ãã³ã¯ããã¢ã¢ã»ãã«
âã»ã«ããŒã¹ãšå ±æçµåã«ããçµåããã®ã§ãç¹
æ®æäœïŒæã¢ã¹ããã·ãã³ã¯BACâã¢ã¹ããã·
ãã³ãšçµåããŠBACâã¢ã¹ããã·ãã³âæã¢ã¹
ããã·ãã³ïŒBACAâæã¢ã¹ããã·ãã³ïŒãç
æãããããã¯æŽæµã«ããBACâã¢ã¹ããã·ã
ã³ããåé¢ãããè¡æž æ®çç©ã詊éšããããšã«ã
ã蚌æããããæšæºãªãŒã¯ã¿ãŒããã€æ¡æ£è©Šéšæ³
ã«ãããè¡æž äžã«ã¢ã¹ããã·ãã³ãšåå¿ãåŸãæ
äœã¯æ®çããŠããªãããšãèªãããããããæ ã
è¡æž äžã«ããããããã®ååšã瀺ãããç¹æ®æäœ
ïŒæã¢ã¹ããã·ãã³ïŒã¯ãã¹ãŠBACâã¢ã¹ããã·
ãã³ã«åžåãããããšãæããã§ãããæŽã«ãæ
ã¢ã¹ããã·ãã³ãBACâã¢ã¹ããã·ãã³ãšã®çµ
åããéæŸãããšããããããã¹ãŠã®æ±ææäœã
ãåé¢ãããããã®æã¢ã¹ããã·ãã³ã®éæŸã¯
0.25Mé ¢é žãšBACAâæã¢ã¹ããã·ãã³ã§æŽæµ
ïŒïŒâã§ïŒæéïŒããããšã«ããè¡ãããšãã§ã
ãã ãŸããè¡æž äžã«ãããç¹æ®æäœã®ååšã¯ç¹æ®æ
äœïŒæããªã°ãã³ãåèšã®ããšãããã¢ã¢ã»ãã«
âã»ã«ããŒã¹âããªã°ãã³ïŒBACâããªã°ãã³ïŒ
äžã«åžåãããããšã«ãããã®èšŒæãåŸãããšã
ã§ãããããªãã¡ãç¹æ®æããªã°ãã³ãå«ãæè¡
æž ãšBACâããªã°ãã³ãåå¿ãããããšãã§ã
ããè¡æž ãBACâããªã°ãã³äžã«éããšããã
ãªã°ãã³ã«å¯Ÿããç¹æ®æäœã®ã¿ãç¹æ®æåããªã°
ãã³ã«çµåãããããªã°ãã³ã¯ããã¢ã¢ã»ãã«â
ã»ã«ããŒã¹ãšå ±æçµåã«ããçµåããã®ã§ãç¹æ®
æäœïŒæããªã°ãã³ã¯BACâããªã°ãã³ãšçµå
ããŠBACâããªã°ãã³âæããªã°ãã³ïŒBACM
âæããªã°ãã³ïŒãçæãããããã¯æŽæµã«ãã
BACâããªã°ãã³ããåé¢ãããè¡æž æ®çç©ã
詊éšããããšã«ãã蚌æããããæšæºãªãŒã¯ã¿ãŒ
ããã€æ¡æ£è©Šéšæ³ã«ãããè¡æž äžã«ããªã°ãã³ãš
åå¿ãåŸãæäœã¯æ®çããŠããªãããšãèªããã
ããããæ ãè¡æž äžã«ããããããã®ååšã瀺ã
ããç¹æ®æäœïŒæããªã°ãã³ïŒã¯ãã¹ãŠBACâ
ããªã°ãã³ã«åžåãããããšãæããã§ãããæŽ
ã«æã¢ã¹ããã·ãã³ãBACâããªã°ãã³ãšã®çµ
åããéæŸãããšããããããã¹ãŠã®æ±ææäœã
ãåé¢ãããããã®æããªã°ãã³ã®éæŸã¯0.25M
é ¢é žãšBACAâæããªã°ãã³ã§æŽæµïŒïŒâã§ïŒ
æéïŒããããšã«ããè¡ãããšãã§ããã æåâæäœåå¿ã®ããã®æšæºçãªãŒã¯ã¿ãŒãã
ã€ã»ã²ã«æ¡æ£è©Šéšæ³ã«ãããã¿ãŒã²ããæåã«ã
ãåŸããããã®ã¿ãŒã²ããã«å¯Ÿããç¹æ®æäœã¯å
èšã®æã¢ã¹ããã·ãã³ãŸãã¯æããªã°ãã³ã®ããš
ãæè¡æž ïŒããªãã¡ã¢ã¹ããã·ãã³ãŸãã¯ããªã°
ãã³èªäœã泚å°ããŠåŸãããæè¡æž ïŒãšåæ§ãã
ã®æåãšã®éã®åå¿ã«ãããåäžçŽç·é¢ä¿ã瀺ã
ããšãæããã§ãããæ³šæãã¹ãããšã¯ãå®¶å ã®
å ã®ãããã®ã¯ã¿ãŒã²ãããæ³šå°ããåã«ããé
ã®æã¿ãŒã²ãããä¿æããŠããããããã®æã¿ãŒ
ã²ããç©è³ªã¯äººè¡æž ã®ãã¹ãã«ãããŠèšèŒããã
ããã«ç¹ã«ã¿ãŒã²ãã詊è¬ãšåå¿ããããšãã
TAGãâTAGããã³ïŒŠâTAGã®çŽåœã¢ã«é
ïŒïŒã¿ã€ãïŒãçæããïŒåŸèšå®æœäŸåç §ïŒã 宿œäŸ ïŒ çç©åŠçæ¶²äœããã¿ãŒã²ããâã¢ã¿ããã³ã°â
ã°ãããªã³ïŒTAGïŒã詊éšç®¡å ã§å®éçã«ç
æãããããšã«ããæªæ§è «çã®æ€åºïŒâ 宿œäŸïŒã«ãã補ããããã¿ãŒã²ãã詊è¬ã®å£
åã«ããååšããããšãããæªçµåã¬ã³ã°ãã³ã
é€å»ããããããããæŽæµããããã®æºè¶³ãã¹ã
æ¹æ³ã¯æ¬¡ã®ãšããã§ãããã¿ãŒã²ãã詊è¬ãé ¢é ž
ãšå ±ã«37âã§ïŒæéæ¹æããé æ²ããäžæŸæ¶²ãåŸ
çåé¢ãããã®äžæŸæ¶²ã®266ïœÎŒã«ãããå åŠå¯
åºŠãæž¬å®ããããããã®æ³¢é·ã«ãããåžåãååš
ãããªãã°ãç©è³ªã溶解ããªããªããŸã§äžèšã®ã
ãšãæŽæµãååŸ©å®æœãããæ¬¡ãã§ãã®ã¿ãŒã²ãã
ããªã³é žç·©è¡é£å¡©æº¶æ¶²ïŒPH7.2ïŒã«åæžæ¿ãã
ïŒã¿ãŒã²ãã詊è¬ã詊éšããããã®æšæºæ³ãåç §
ããããä»ã®ã¿ãŒã²ãã補å€ã«ããâTAGã
ãã³ïŒŠâTAGãå«ãããšã詊éšã«ããæãããš
ãªã€ãå®¶å å šè¡æž ãåŸãããšãã§ãããªãã°ãåŸ
èšæ¹æ³ã«ããäººè¡æž ã®åå¿ãã粟補ããæšæºç
âTAGããã³ïŒŠâTAGã䜿çšããããšãã§ã
ãïŒã æ¬¡ã®æ¹æ³ã«ããã¹ããŒâãã€ã³ãã³ã°ïŒïŒ³â
TAGïŒã決å®ããïŒæ°æ¥éä¿åããåçµè¡æž ã¯
ããã䜿çšãã¹ãã§ãªããåžžå¥ã®æ¹æ³ã«ãããæ°
ããåŸãããå šè¡æ¶²ãããã¯ä»ã®äœæ¶²ããæ³šæã
ãªããè¡æž ãã€ãããæºè¶³ãã¹ãæ¹æ³ãè¿°ã¹ãã°
次ã®ãšããã§ãããæ¡åããè¡æ¶²ãã¬ã©ã¹è£œè©Šéš
管äžã宀枩ã§ïŒæéæŸçœ®ããåè¡ããããã¬ã©ã¹
è£œæ¹ææ£ãçšããåè¡ã詊éšç®¡å£ããåé¢ããã
ã®è¡æ¶²ãïŒâã§æäœïŒæéïŒãããã¯äžå€ïŒæŸçœ®
ãããïŒâã§45åéé å¿åé¢ïŒ20000rpmïŒãã
ããšã«ããåè¡ãè¡æž ããåé¢ããè¡æž ãé å¿å
é¢ç®¡äžã«åŸçåé¢ããæŽã«ïŒâã§45åéé å¿åé¢
ïŒ2000rpmïŒããããã®è¡æž ãåŸçããã¡ããªã¬
ãŒãïŒïŒ æº¶æ¶²ïŒæ°Ž95mlããã³0.2Mçé žæ°ŽçŽ å¡©ç·©
è¡æ¶²ïŒPH10ïŒïŒmläžãïŒïœïŒãè¡æž ã«å¯ŸããŠïŒïŒ
ïŒå®¹éïŒã®é床ã§å ããã äžè𿹿³ãŸãã¯ä»ã®æ¹æ³ã§åŸãããè¡æž ããã
ã0.2mlããã¿ãŒã²ãã詊è¬0.25mlåœãã¬ã³ã°ã
ã³100ã200mg嫿ã®ã¿ãŒã²ããæžæ¿æ¶²è©Šè¬ããã
ã0.25mlã«å ããïŒåã®æ€æ»è©Šæãšããããã¬ã
ãçæãé¿ãããããããªæ¹æ³ã«ãããäžèšæžæ¿
æ¶²ãïŒâã§æ··åãããããšãã°å°ãããŽã 補ãã€
ãããçšããŠïŒãïŒç§éåŒ·ãæ¯ããŸããåŸã管ã
ããããã«åŸçãããŠãããããŒãã¹ã·ãšãŒã«
äžãçŽïŒæéãŸãã¯ããä»¥äžæ¯çªããããã¿ãŒã²
ãã詊è¬ãšããã«çµåããèçœãè¡æž ããåé¢ã
ãããã®çºæã§èŠåºãããæºè¶³ãã¹ãæ¹æ³ã«ã
ããäžèšç®¡ãïŒâã§20åéé æ²ïŒ2000rpmïŒãäž
æŸãåŸçããåŸãããã0.15Mé£å¡©æ°Ž0.2ã0.3ml
ãšåæ··åããå®€æž©ã§æ¯çªããé æ²ããŠäžæŸæ¶²ãå
é¢ããæäœãïŒåè¡ãããšã«ããçæãããã¬ã
ããæŽæµããã ã¿ãŒã²ããã«ä»çããŠæ®çããèçœããããã
åé¢ãããã®èçœãå®éãããããšãã°0.25Mé ¢
é ž0.2mlãå ãããã®æžæ¿æ¶²ã37âã®ãµåµåšäžã§
ïŒãïŒæéæ¯ããŸããã管ãïŒâã§30åéé æ²
ïŒ2000rpmïŒãããç²ç¶ç©ãç§»è¡ããã®ãé¿ãã
ããã«æ³šæããªããäžæŸæ¶²ãåŸçãã280ïœÎŒã«
ãããäžæŸæ¶²ã®å åŠå¯åºŠã枬å®ãããè¡æž èçœ
ïŒïŒ³âTAGïŒmlåœãmgã®çµæãåŸããããäžèšå
åŠå¯åºŠã®æž¬å®å€ã1.46ã®å åã§å²ããæ€æ»è³æïŒ
åã®éã«ïŒïŒ 以äžã®å·®ããã€ãŠã¯ãªããªããèçœ
å«éãæ€æ»ãããããä»ã®æ¹æ³ããšãã°ããªãªã³
âããŠãªã€æ€æ»æ³ãçšããããšãã§ããããå¯Ÿç §
詊æã®æ¿åºŠãšïŒïŒ³âTAGïŒâïŒïŒŠâTAGïŒæ¿åºŠã®
å€ã®ç¯å²ãæšæºæ³ã«ãããŠç¹å®ããŠãããã°ãªã
ãªãã ãã¢ã¹ãâãã€ã³ãã³ã°ïŒïŒŠâTAGïŒå€ã¯æ¬¡
ã®ããã«æ€æ»ããïŒæ°æ¥ä»¥äžåçµããŠä¿åããã
è¡æž ã¯äœ¿çšãã¹ãã§ã¯ãªããåžžå¥ã®æ¹æ³ã«ããã
æ°ããåŸãããå šè¡æ¶²ãããã¯ãã®ä»ã®äœæ¶²ãã
泚æããŠè¡æž ãäœæãããè¡æž ãã€ããæ¹æ³ã¯ã
ã®å®æœäŸã®åéšã«èšèŒããæ¹æ³ã§å åã§ããã äžèšã®åã¯ä»ã®ææ³ã§äœæãããè¡æž èŠæ¬ã¯ã
âTAGè¡æž 枬å®å€ãäžèšã®TARGET詊å€ãš
æ¥è§Šãããããå šæéããã10åéå°ãªãæéã®
éãïŒâã§æŸçœ®ããïŒäŸãã°ãïŒæéãã®ïŒ³â
TAG枬å®ããããªãã°ïŒæé50åïŒããã®æ¹æ³ã§
ã¯ïŒ³âTAGãšïŒŠâTAGã®æž¬å®å€ã®æž©åºŠå±¥æŽãå¹³
è¡¡ãããã æž©åºŠå¹³è¡¡ããè¡æž ã®èŠæ¬0.2mlããäºé枬å®ã«
ãããŠãTARGET詊å€0.25mlã«ã€ã
RECOGNIN100ä¹è³200ãã€ã¯ãã°ã©ã ã嫿ã
ãTARGETããæ¿è©Šå€ã®0.25mlæŽé€æ°ã®ããã
ãã«å ãããããæ¿ç©ã¯ãé¡ç²åœ¢æãé¿ãããã
ã«ããŠçŽ10åéïŒâã§æ··åãããäŸãã°ãæ¥éã«
æ¯ãåãããå°ãŽã ãã€ãããïŒãïŒç§éçšã
ãŠããããã管ãå ãã«åŸããŠããããããŒãã¹
æ¯ãšãåšã§çŽ10å鿝ãšãããã°ãããããã«ã€
ããŠããTARGET詊å€åã³èçœè³ªã¯è¡æž ããå
é¢ããããæºè¶³ã§ãããšèªããããææ³ã®äžã€ã¯
äžèšã®ãã®ã§ãããæ¬¡ã«ç®¡ã2000rpmã§20åéïŒ
âã§é å¿åé¢ããäžæŸæ¶²ãéæ³šããé å¿åé¢ã§åœ¢
æããé¡ç²ã0.15Må¡©æ°Ž0.2ã0.3mlãšåæ··åã宀
æž©ã§æ¯ãšãããããšã«ãã€ãŠïŒåæŽæµããé å¿å
é¢ããŠãäžæŸæ¶²ã廿£ããã æšçã«ä»çãããŸãã«ãªã€ãŠããèçœè³ªããã
ããéè£ããå®éçã«æž¬å®ãããâTAGæ¿åºŠ
ãæž¬å®ããããã®æ¬èŠæ¬ã«ãããäžèšã®ææ³ã¯æº
è¶³ãªãã®ã§ãããããªãªã³ã»ããŠãªãŒæž¬å®æ³ã®ã
ããªãèçœè³ªå«æéãæž¬å®ããã®ã«å¹æã®ããä»
ã®ææ³ãçšããŠãããããããå¶åŸ¡ã®ç¯å²åã³ïŒ³
âTAGãã€ãã¹ïŒŠâTAGæ¿åºŠã®è «çå€ã決å®ã
ãããåºæºãå®ããªããã°ãªããªãã æçµçµæã¯è¡æž ïŒmlã«ã€ãTAGãã€ã¯ãã°ã©
ã ãšããŠè¡šããããããã¯ïŒ³âTAGãã€ãã¹ïŒŠ
âTAGã«çãããéè³è «çæ£è åã³ä»ã®å¶åŸ¡ã«
ãããTAGå€ã¯çŸåšã®ãšããè¡æž ïŒmlã«ã€ããŒ
ãïŒåã¯è² æ°ïŒãã135ãã€ã¯ãã°ã©ã ãŸã§ã®ç¯
å²ã«äºããïŒmlã«ã€ã100ïœÎŒãè¶ããçµæã«ã€
ããŠã¯ãåèŠæž¬å®ã衚瀺ãããä»ã®è «çã®ããã
ã®ã¯é«ãTAGå€ã衚ããããšããããç¹ã«è³å
ã«äºæ¬¡çïŒè»¢ç§»ããïŒè «çãããå Žåã«ããã§ã
ããè³è «çæ£è ã®TAGå€ã¯çŸåšã§ã¯è¡æž ïŒmlã«
ã€ã136ä¹è³500以äžãã€ã¯ãã°ã©ã ã«åã¶ãã¢ã¹
ããã·ãã³åã³ããã¢ã¢ã»ãã«ã»ã«ããŒãºãã調
æŽããTARGET詊å€ãå©çšããæ¬å®æœäŸã®ææ³
ã«åŸã€ãŠè¡ãªã€ã50ã®è¡æ¶²èŠæ¬ã«ã€ããŠã®æåã®
ãç²æ€ãã«ãããŠã¯ã11ã®è³è «çã®ãã¡11ãåã³
32ã®æ£åžžã®ãã®ããã¡28ãæ£ç¢ºã«å€å¥ããããïŒ
ä»¶ã®æ£åžžãšæãããŠãããã®ïŒå³ã¡ãéè³è «çå¶
埡ïŒã®ãã¡ïŒä»¶ã¯ãæããã«äœå¹Žãåã«è¯ãæåœ
ããç²ç¶è ºçã®ããããšã倿ãããæ®ãã®ïŒä»¶
ã®æ£åžžè ã¯å¥åº·ã®ããããªããå€å蚺æã®ã€ããŠ
ããªãçãæã€ãã幎什60ã70ã®å人ã§ãã€ãã
æ®ãã®ïŒäŸã®ãã¡ãããžãã³ã¹ç ã®ïŒä»¶äžã®ïŒä»¶
ã¯æ£ç¢ºã«å€å¥ããããè «çã®ç¯å²ã«å ¥ãïŒäŸ
ïŒ136ã500ÎŒïœTAGïŒmlïŒã¯ã²ã©ãç¥çµè çãæ
ã€æ£è ã«è©²åœããéè «çã®ç¯å²ïŒïŒã135ÎŒïœ
TAGïŒmlïŒã«å ¥ãïŒäŸã¯ãããããäžæç¢ºã§ã¯
ãããè «çã§ã¯ãªãé èå å¡ã®èšºæãåã³éªšèè «
ïŒéè³è «çïŒåã³é»ç®èè «ïŒéè³è «çïŒãæã€ã
æ£è ã«çžåœããã MALIGNINåã³æªæ§è³è «çåã³å šæ£åžžè ãã
調補ããTARGET詊å€ãå©çšããŠæ¬å®æœäŸã®æ
æ³ã«åŸã€ãŠãã®åŸã®æ€èšãè¡ãªã€ãã æ¬å®æœäŸã®ææ³ã«åŸã€ãŠè¡ãªã€ãæŽã«ãã以äž
ã®æ€èšã§ã¯è©Šéšãã人éã®è «çã®èŠæ¬ã®ç·æ°ã50
ãã114ã«åãã ããããã®è£œååã³ææ³ã®æçš
æ§ã第ïŒè¡šã«ç€ºãããããã¯ãããã®è©Šéšã®çµæ
ãèšé²ãããã®ã§ããã 宿œäŸ ïŒ ã€ã ããã«ãªã¬ã³ã»ã³ã¹ã«ããè «ç现èã®èšºæ ååç©æâã¢ã¹ããã·ãã³ãæâããªã°ãã³ã
ãã³ïŒ³âTAGã¯è «ç现èã«åªå çã«ä»çããã
ãšã瀺ãããã®ç¹ç°æ§ã¯çµç¹åŠã®éšéã«ãããŠæ
æãŸãã¯æŸå°æ§ç©è³ªãæâã¢ã¹ããã·ãã³ãæâ
ããªã°ãã³ãŸãã¯ïŒ³âTAGã«çµåãããŠããã
ã®ååç©ãè «ç现èã®èšºæã«çšãåŸããæšæºã®æš
èæè¡ãããããçšãããããâTAGã®äœ¿çš
æ³ã¯æ¬¡ã®ãšããã§ããã æºè¶³ã§ãããšèªããããäžã€ã®æ¹æ³ã¯ã»ã³ãã»
ããªãŒïŒSt.MarieïŒæ³ã®å€åœ¢ã§ããã人éã®è³
è «çæ€äœãåçµãïŒãã¯ãã³ã®åãã«åãããã
ããæ¹¿å®¹åšäžã«æè²åã«â70âã«ïŒãïŒé±é貯ã
ããçµåäœã¯å±±çŸã®æâãŠãµã®çµåäœã®ãããªæš
æºã®æè¡æž ã§ãããçµåäœã¯ãã«ãªã¬ãã»ã³ããŸ
ãã¯ä»ã®æšæºç©è³ªã§ãã®æè¡åéã§æ¢ç¥ã®ææ³ã«
ãã€ãŠæšèããããåžè²©ã®ãã«ããšãã»ã€ã³æšè
å±±çŸæâãŠãµã®çµåäœãçšãåŸããçšãããã«ãª
ã¬ãã»ã³ãææ³ã¯æšæºã®ãã®ã§ãã€ãŠãTAGã®
ïŒïŒ200ãïŒïŒ400溶液ãè «çåçäžã«30åéãŸã
ã¯ãã以äžä¿æž©ããæ¬¡ã«æŽæµããŠä»çããªã
TAGãé€ããçé žç·©è¡é£å¡©æ°Žã§ïŒåæŽæµããã®
ãããããã«ãªã¬ãã»ã€ã³âæšèçµåäœã§çµåä¿
æž©ããæŽæµãããé¡åŸ®é¡æ€æ»ããããæ£èŠã®çްè
ããã³ãã®çªèµ·ã¯è «çåçããã³æ£èŠã®éè «çè³
ã®å¯Ÿç §åçå ±ã«æè²ãããªãããã«ãªã¬ãã»ã³ã¹
ã¯è «çç¥çµè ããã³ãã®çªèµ·ã«æããååšããã 宿œäŸ 10 çµç¹å¹é€ã§å¢æ®ããè «ç现èã«å¯ŸããŠæâã¢ã¹
ããã·ãã³ãæâããªã°ãã³ããã³ïŒ³âTAG
ãçŽ°èæ¯æ§ãæããããšã®å®éš çŽ°èæ¯æ§ã枬å®ããæšæºè©Šéšãçšãããäžè¬
ã«ãåºå®ããèšç®ç®±äžã®çްèã®æ°ãæ®éçŽ100ã®
çãã现èã嫿ããããã«æºåããããããã
ãèšç®ãããæ¬¡ã«ãããã®çްèã詊éšãã詊å€ã§
åŠçãããªãçããŠãã现èã®æ°ãèšç®ããã 宿œäŸïŒã®æ¹æ³ã§åŸãããâTAG溶液ã®ã
ç¥çµè çºçãååã«ç¹åŸŽã¥ãããããç¥çµè èœçް
èãçŽã®æ£è ããã®çµç¹å¹é€äžã®çްèã«å¯Ÿã
ãçŽ°èæ¯æ§ã®æšæºè©Šéšã«ãããŠãâTAGã¯æº¶
æ¶²mlåœãâTAG0.2ããã³0.02ÎŒïœã®ãããªå°é
ã«çžåœããïŒïŒ100ããã³ïŒïŒ1000ã®é«åžé床ã®
å Žåã§ãèšç®ç®±äžã®ãã¹ãŠã®çްèãæ»æ» ãããã
åæ§ãªçµæãæâã¢ã¹ããã·ãã³ããã³æâããª
ã°ãã³ã®é«åžéæ¶²ã§åŸãããã 宿œäŸïŒã§ç€ºãç¹ç°æ§ããã³å®æœäŸ10ã§ç€ºã现
èæ¯æ§ã¯å ±ã«äººéã®è³è «çã«å¯Ÿããæâã¢ã¹ãã
ã·ãã³ãæâããªã°ãã³ããã³ïŒ³âTAGã®æ²»ç
å¯èœæ§ã«éåžžã«é¢ä¿ãããããããã®æ²»çç䜿çš
ã¯æè¡ã§é€å»ãããè «ççµç¹ã«å¯Ÿãããããã®äž¡
æ¹ã®çŸè±¡ã®å®éã®èšºæå¯èœæ§ã«å ããŠæ¢ã«èšŒæã
ãçµç¹åŠã«ãã蚺æãå¿ èŠãšããã
衚é¢ç©ãå¢å ããããããšã«ãããïŒæ¥çºè²æé
åœããæªæ§çްè墿®éïŒæªæ§ã®åºŠåïŒãå¢å€§ãã
ã«åŸã€ãŠç·èçœã®ïŒ ãšããŠç€ºãããçæããªã°ã
ã³éããŸãææã«å¢å€§ãããïŒæ¥ã®çºè²æéäžã«
ç·èçœéã«å¯Ÿããããªã°ãã³ã®çŸåçã¯250mlã
ã©ã¹ã³äœ¿çšæã«ãããå¹³åå€10.7ïŒ ããã1000ml
ãã©ã¹ã³äœ¿çšæã«ãããå¹³åå€28.3ïŒ ã«å¢å ã
ãã çæããããªã°ãã³ã®å²åãšæªæ§ã®åºŠåïŒããª
ãã¡ïŒæ¥é詊éšç®¡å ã«ãããæªæ§çްè墿®éã«å¯Ÿ
ããçæèçœéã®é¢æ°ïŒãšã®é¢ä¿ã第ïŒå³ã«ç€º
ãã 第ïŒå³ã¯ãã©ã¹ã³ã®å€§ãããå¢å ããã®ã§ããª
ã°ãã³çæç©ã®æ¯çã¯çŽïŒåã«å¢å€§ããããšã瀺
ããŠããããŸã第ïŒå³ã¯ããªã°ãã³ãšæªæ§ã®åºŠå
ãšã®é¢ä¿ã瀺ããã®ã§ãããäžéç·ã¯çæ³ççŽç·
é¢ä¿ã瀺ãç·ã§ããããã®å®èšŒçé¢ä¿ã¯ãã®çްè
ã®å¢æ®ãïŒç çåŠçã«ïŒãã黿¢ãåŸãªããã®ãš
ãªãã«åŸã€ãŠããªã°ãã³ã®ååšæ¯çãå¢å€§ããã
ã®ã§ããããéèŠãªé¢ä¿ã§ãããéåžžã®ã¬ã³ã°ã
ã³ã®æ©èœããèªäœã¯ãåèšã®ããšã现èã«æ¥è§Šã
ãŠãã®å¢æ®ãæå¶ããããšã«é¢é£æ§ãæãããæª
æ§çްèã®å¢æ®ãããç çã§ããã°ãæ¥è§Šã«ããæ
æ§å¹æã¯æžå°ããããªã°ãã³ãããåªå çãªèçœ
ãšãªã€ãŠå¢å ããã 宿œäŸ5Aã«ãããŠã¯ã倧ãªãçºè²å®¹åšäžã§çº
è²ããã人工çç现èå¹é€ç©ã¯äºæã«åããŠçæ
ããªã°ãã³ã®å Žåãããªãã¡ç·èçœéäžã®ããªã°
ãã³ã®çŸåçãå¢å€§ããããšãäŸèšŒãããã®ã§ã
ãããã®çºæã«ãããŠçšãããããããªå€§å®¹éã®
çºè²å®¹åšã¯å®¹åšå®¹éã®å®æœäŸïŒã«ãã现èå ¥å¹å°
ç·å®¹éã«å¯Ÿããæ¯ãçŽïŒïŒïŒïŒããšãã°ãã®ç¯å²
ã¯ïŒïŒïŒãªãã10ïŒïŒïŒãã倧ãªã容åšã§ããã
ãšãæå³ããã宿œäŸ5Aã«ãããŠã¯ãã®æ¯ã¯çŽ
ïŒïŒïŒã§ãã€ãã 宿œäŸ ïŒ ã¬ã³ã°ãã³é¡ããã¿ãŒã²ãã詊è¬ã®è£œé ïŒâ åèšå®æœäŸïŒã§è£œããããã¢ã¹ããã·ãã³ãŸã
ã¯å®æœäŸïŒã§è£œããããããªã°ãã³ãæ äœãšæ··å
ããŠã¿ãŒã²ãã詊è¬ã補ããã 奜ãŸãã宿œæ æ§ã«ãããŠãã¢ã¹ããã·ãã³ãŸ
ãã¯ããªã°ãã³ã0.15Mãªã³é žäºæ°ŽçŽ ãããªãŠã
âã¯ãšã³é žç·©è¡æ¶²ïŒPH4.0ïŒã«æº¶è§£ãããããã¢
ã¢ã»ãã«æš¹èãããšãã°ã»ã«ããŒã¹ïŒïœåœãèçŽ
1.0ã1.5ããªã¢ã«ãæããããã¢ã¢ã»ãã«ã»ã«ã
ãŒã¹ïŒBACïŒã0.15Mãªã³é žäºæ°ŽçŽ ãããªãŠã
ç·©è¡æ¶²ïŒPH7.2ïŒäžã§è£œããå·æã«è²¯ãããPH7.2
ã®ç·©è¡æº¶æ¶²ã泚ããäžèš0.15Mãªã³é žäºæ°ŽçŽ ãã
ãªãŠã âã¯ãšã³é žç·©è¡æ¶²ïŒPH4.0ïŒãå ããããš
ã«ãããåŸãããç·©è¡æ¶²ã®PHãïŒã«èª¿ç¯ãããã¢
ã¹ããã·ãã³ãŸãã¯ããªã°ãã³æº¶æ¶²ãšBACã®æº¶
æ¶²ïŒBACã®ã¬ã³ã°ãã³ã«å¯Ÿããæ¯10ïŒïŒïŒã宀
æž©ã§30æéæ¹æããåŸãé å¿åé¢ããã ã¬ã³ã°ãã³ã«çµåãããããã«çšããBACäž
ã®ãã¹ãŠã®ãµã€ãã¯çµåã«é¢äžããŠããããšã奜
ãŸãããããã¯æ¬¡ã®ããã«ããŠéæããããçŽäž
ã«èšèŒããæ®µéã«ãããŠåŸãããäžæŸæ¶²ãåçµä¹Ÿ
ç¥ããBACã«çµåããŠããªãã¢ã¹ããã·ãã³ãŸ
ãã¯ããªã°ãã³ã®éãç¥ãããã«èçœå«éãæž¬å®
ãããè€åBACâã¢ã¹ããã·ãã³ïŒãŸãã¯BAC
âããªã°ãã³ïŒãå床0.1Mçé žæ°ŽçŽ å¡©ç·©è¡æ¶²
ïŒPH8.9ïŒã«æžæ¿ããïŒâã§24æéæ¹æããŠBAC
ããã³ã¢ã¹ããã·ãã³ãŸãã¯ããªã°ãã³ã®éã®å
åŠçµåã圢æãããã24æéåŸããã®æžæ¿æ¶²ãé
å¿åé¢ããäžæŸæ¶²ã®èçœãåæãããè€åBAC
âã¢ã¹ããã·ãã³ãŸãã¯BACâããªã°ãã³ãæŽ
ã«0.05Mã¢ãããšã¿ããŒã«â0.1Mçé žæ°ŽçŽ å¡©ç·©
è¡æ¶²ïŒPH8.9ïŒäžã«åæžæ¿ããŠæªåå¿èçŽ ãéå¡
ããããã®æžæ¿æ¶²ãé å¿åé¢ããã¢ãããšã¿ããŒ
ã«ãååšãããããã®äžæŸæ¶²ã¯ãããåæã«ä»ã
ãããšãªããã®ãŸãŸä¿æããããããé å¿åé¢
ããã¹ãã¯ããããã¡ãŒã¿ã§266ïœÎŒã«åžåã芳
枬ãããªããªããŸã§ãã®åæžæ¿æ¶²ã0.15Må¡©åã
ããªãŠã ã§ïŒåæŽæµããããã®BACâã¢ã¹ãã
ã·ãã³ãŸãã¯BACâããªã°ãã³è€åäœã8Må°¿çŽ
äžã38âã§ïŒæéæ¹æããé å¿åé¢åŸãæŽæ¶²äž
ã«ã266ïœÎŒã®åžåãçŸãããªããªããŸã§ïŒéåžž
ïŒåïŒã8Må°¿çŽ ã§æŽæµããããã®è€åäœã0.15M
å¡©åãããªãŠã ã§ïŒåæŽæµããŠå°¿çŽ ãé€å»ããã
ãã®è€åäœã0.25Mé ¢é žäžã37âã§ïŒæéæ¹æ
ãããã®å®å®æ§ã蚌æãããé å¿åé¢ããåŸãã
ãäžæŸæ¶²ã¯å¿ ç¶çã«266ïœÎŒã«ãããåžåãååš
ããªããããæ ããã®ååŠçè€åäœïŒBACâã¢
ã¹ããã·ãã³ãŸãã¯BACâããªã°ãã³ã¯å®å®ã§
ãã€ãŠäžèšã®ããšãæ¹æ³ã«ããã詊è¬ãšããŠçšã
ãããšãã§ããããã®å®å®ãªè©Šè¬åã«ãããŠãã
ããåææ³ã«ããåŸãããé¯äœãšåŒã¶ããšãã§
ãããã®ç©ççããã³ååŠçæ§è³ªã¯æœåšçåå¿ç¶
æ ã«ãããŠè¡æž æåãšããŠçè§£ãããæãå®å®çް
èâã¢ã¹ããã·ãã³ãŸãã¯ããªã°ãã³ã®çµååé§
äœãšèŠå£ãããšãã§ãããä¿åã®ããã«ã¯ãã®ã¿
ãŒã²ãã詊è¬ãé å¿åé¢ãã0.15Mãªã³é žäºæ°ŽçŽ
ãããªãŠã ç·©è¡æ¶²ïŒPH7.2ïŒã§äžæ§ã«ãªããŸã§æŽ
ãã ã¿ãŒã²ãã詊è¬ã¯ã»ã«ããŒãºä»¥å€ã®é äœåãæ
ããããã¢ã¢ã»ãã«æ äœãããšãã°ããã¢ã¢ã»ã
ã«åæš¹èãããã¯çŽããã§ããã䜿çšããããš
ãã§ããã 宿œäŸ ïŒ ã¢ã¹ããã·ãã³ãããªã°ãã³ããã³ã¿ãŒã²ãã
ã«å¯Ÿããæè¡æž ã®è£œé ïŒâ ã¢ã¹ããã·ãã³ãããªã°ãã³ãŸãã¯ã¿ãŒã²ãã
詊è¬ã«å¯Ÿããæè¡æž ã¯åºä¹³åç©ã«ãããããã詊
è¬ã«å¯Ÿããæäœåå¿ãèªçºããããšã«ãã補ãã
ããããã®ç®çã®ããã«æºè¶³ãªæ¹æ³ã以äžèª¬æã
ãã ã¬ã³ã°ãã³ïŒã¢ã¹ããã·ãã³ããã³ããªã°ã
ã³ïŒïŒmgãæšæºããã€ã³ãäœè¬ãšå ±ã«çœè²éå®¶å
ã®è¶³ã®èè¶Ÿã«æ³šå°ããïŒé±éåŸãæŽã«10æ¥åŸãèŠ
ããã°æŽã«ïŒé±éåŸè ¹è å ã«åæ§ã®æ³šå°ãè¡ãã
æåã®æ³šå°ããïŒé±éãªãã10æ¥åŸãæ©ãããã
ã®å®¶å ã®è¡æž äžã«ç¹æ®ãªæäœãæ€åºããããšãã§
ãããã¿ãŒã²ããæåãåŸããããã¢ã¹ããã·ã
ã³ãŸãã¯ããªã°ãã³ïŒmgïŒããªãªã³âããŠãªã€è
çœæ€åºæ³ã«ããæž¬å®ïŒã嫿ããã¿ãŒã²ããéã
泚å°ããæäœãäžèšåæ§ã®æ¹æ³ã«ããè¡ããã¢ã¹
ããã·ãã³ã«å¯Ÿããç¹æ®æäœãæã¢ã¹ããã·ãã³
ïŒAntiâAstrocytinïŒãšåŒã¶ãããªã°ãã³ã«å¯Ÿã
ãç¹æ®æäœãæããªã°ãã³ïŒAntiâMaligninïŒ
ãšåŒã¶ãåæ§ã«ã¿ãŒã²ãã詊è¬ã«å¯Ÿããç¹æ®æäœ
ãæã¿ãŒã²ããïŒAntiâTargetïŒãšåŒã¶ã æåâæäœåå¿ã®ããã®æšæºçãªãŒã¯ã¿ãŒãã
ã€ïŒOuchterlonyïŒã²ã«æ¡æ£è©Šéšæ³ã«ããããã®
ç¹æ®æåã«ããåŸãããç¹æ®æäœã¯ãã®æåãšã®
åå¿ã«ãããåäžéæãªçŽç·é¢ä¿ã瀺ãããšãæ
ããã§ããã è¡æž äžã«ãããç¹æ®æäœã®ååšã¯æåâæäœå
å¿ã®ããã®æ²éçŽ æšæºå®éè©Šéšæ³ã«ãã€ãŠã詊éš
ããããšãã§ããããã®è©Šéšã«ããè¯å¥œãªæ²éçŽ
å®éæ²ç·ãåŸãããšãã§ããããããç¹æ®æäœã®
mgæ°ãèšç®ããããšãã§ããã æŽã«è¡æž äžã«ãããç¹æ®æäœã®ååšã¯ç¹æ®æ
äœïŒæã¢ã¹ããã·ãã³ãåèšã®ããšãããã¢ã¢ã»
ãã«âã»ã«ããŒã¹âã¢ã¹ããã·ãã³ïŒBACâã¢
ã¹ããã·ãã³ïŒäžã«åžåãããããšã«ãããã®èšŒ
æãåŸãããšãã§ãããããªãã¡ãç¹æ®æã¢ã¹ã
ãã·ãã³ã嫿ããæè¡æž ãšBACâã¢ã¹ããã·
ãã³ãåå¿ãããããšãã§ãããè¡æž ãBACâ
ã¢ã¹ããã·ãã³äžã«éããšããã¢ã¹ããã·ãã³ã«
察ããç¹æ®æäœã®ã¿ããã®ç¹æ®æåã¢ã¹ããã·ã
ã³ã«çµåãããã¢ã¹ããã·ãã³ã¯ããã¢ã¢ã»ãã«
âã»ã«ããŒã¹ãšå ±æçµåã«ããçµåããã®ã§ãç¹
æ®æäœïŒæã¢ã¹ããã·ãã³ã¯BACâã¢ã¹ããã·
ãã³ãšçµåããŠBACâã¢ã¹ããã·ãã³âæã¢ã¹
ããã·ãã³ïŒBACAâæã¢ã¹ããã·ãã³ïŒãç
æãããããã¯æŽæµã«ããBACâã¢ã¹ããã·ã
ã³ããåé¢ãããè¡æž æ®çç©ã詊éšããããšã«ã
ã蚌æããããæšæºãªãŒã¯ã¿ãŒããã€æ¡æ£è©Šéšæ³
ã«ãããè¡æž äžã«ã¢ã¹ããã·ãã³ãšåå¿ãåŸãæ
äœã¯æ®çããŠããªãããšãèªãããããããæ ã
è¡æž äžã«ããããããã®ååšã瀺ãããç¹æ®æäœ
ïŒæã¢ã¹ããã·ãã³ïŒã¯ãã¹ãŠBACâã¢ã¹ããã·
ãã³ã«åžåãããããšãæããã§ãããæŽã«ãæ
ã¢ã¹ããã·ãã³ãBACâã¢ã¹ããã·ãã³ãšã®çµ
åããéæŸãããšããããããã¹ãŠã®æ±ææäœã
ãåé¢ãããããã®æã¢ã¹ããã·ãã³ã®éæŸã¯
0.25Mé ¢é žãšBACAâæã¢ã¹ããã·ãã³ã§æŽæµ
ïŒïŒâã§ïŒæéïŒããããšã«ããè¡ãããšãã§ã
ãã ãŸããè¡æž äžã«ãããç¹æ®æäœã®ååšã¯ç¹æ®æ
äœïŒæããªã°ãã³ãåèšã®ããšãããã¢ã¢ã»ãã«
âã»ã«ããŒã¹âããªã°ãã³ïŒBACâããªã°ãã³ïŒ
äžã«åžåãããããšã«ãããã®èšŒæãåŸãããšã
ã§ãããããªãã¡ãç¹æ®æããªã°ãã³ãå«ãæè¡
æž ãšBACâããªã°ãã³ãåå¿ãããããšãã§ã
ããè¡æž ãBACâããªã°ãã³äžã«éããšããã
ãªã°ãã³ã«å¯Ÿããç¹æ®æäœã®ã¿ãç¹æ®æåããªã°
ãã³ã«çµåãããããªã°ãã³ã¯ããã¢ã¢ã»ãã«â
ã»ã«ããŒã¹ãšå ±æçµåã«ããçµåããã®ã§ãç¹æ®
æäœïŒæããªã°ãã³ã¯BACâããªã°ãã³ãšçµå
ããŠBACâããªã°ãã³âæããªã°ãã³ïŒBACM
âæããªã°ãã³ïŒãçæãããããã¯æŽæµã«ãã
BACâããªã°ãã³ããåé¢ãããè¡æž æ®çç©ã
詊éšããããšã«ãã蚌æããããæšæºãªãŒã¯ã¿ãŒ
ããã€æ¡æ£è©Šéšæ³ã«ãããè¡æž äžã«ããªã°ãã³ãš
åå¿ãåŸãæäœã¯æ®çããŠããªãããšãèªããã
ããããæ ãè¡æž äžã«ããããããã®ååšã瀺ã
ããç¹æ®æäœïŒæããªã°ãã³ïŒã¯ãã¹ãŠBACâ
ããªã°ãã³ã«åžåãããããšãæããã§ãããæŽ
ã«æã¢ã¹ããã·ãã³ãBACâããªã°ãã³ãšã®çµ
åããéæŸãããšããããããã¹ãŠã®æ±ææäœã
ãåé¢ãããããã®æããªã°ãã³ã®éæŸã¯0.25M
é ¢é žãšBACAâæããªã°ãã³ã§æŽæµïŒïŒâã§ïŒ
æéïŒããããšã«ããè¡ãããšãã§ããã æåâæäœåå¿ã®ããã®æšæºçãªãŒã¯ã¿ãŒãã
ã€ã»ã²ã«æ¡æ£è©Šéšæ³ã«ãããã¿ãŒã²ããæåã«ã
ãåŸããããã®ã¿ãŒã²ããã«å¯Ÿããç¹æ®æäœã¯å
èšã®æã¢ã¹ããã·ãã³ãŸãã¯æããªã°ãã³ã®ããš
ãæè¡æž ïŒããªãã¡ã¢ã¹ããã·ãã³ãŸãã¯ããªã°
ãã³èªäœã泚å°ããŠåŸãããæè¡æž ïŒãšåæ§ãã
ã®æåãšã®éã®åå¿ã«ãããåäžçŽç·é¢ä¿ã瀺ã
ããšãæããã§ãããæ³šæãã¹ãããšã¯ãå®¶å ã®
å ã®ãããã®ã¯ã¿ãŒã²ãããæ³šå°ããåã«ããé
ã®æã¿ãŒã²ãããä¿æããŠããããããã®æã¿ãŒ
ã²ããç©è³ªã¯äººè¡æž ã®ãã¹ãã«ãããŠèšèŒããã
ããã«ç¹ã«ã¿ãŒã²ãã詊è¬ãšåå¿ããããšãã
TAGãâTAGããã³ïŒŠâTAGã®çŽåœã¢ã«é
ïŒïŒã¿ã€ãïŒãçæããïŒåŸèšå®æœäŸåç §ïŒã 宿œäŸ ïŒ çç©åŠçæ¶²äœããã¿ãŒã²ããâã¢ã¿ããã³ã°â
ã°ãããªã³ïŒTAGïŒã詊éšç®¡å ã§å®éçã«ç
æãããããšã«ããæªæ§è «çã®æ€åºïŒâ 宿œäŸïŒã«ãã補ããããã¿ãŒã²ãã詊è¬ã®å£
åã«ããååšããããšãããæªçµåã¬ã³ã°ãã³ã
é€å»ããããããããæŽæµããããã®æºè¶³ãã¹ã
æ¹æ³ã¯æ¬¡ã®ãšããã§ãããã¿ãŒã²ãã詊è¬ãé ¢é ž
ãšå ±ã«37âã§ïŒæéæ¹æããé æ²ããäžæŸæ¶²ãåŸ
çåé¢ãããã®äžæŸæ¶²ã®266ïœÎŒã«ãããå åŠå¯
åºŠãæž¬å®ããããããã®æ³¢é·ã«ãããåžåãååš
ãããªãã°ãç©è³ªã溶解ããªããªããŸã§äžèšã®ã
ãšãæŽæµãååŸ©å®æœãããæ¬¡ãã§ãã®ã¿ãŒã²ãã
ããªã³é žç·©è¡é£å¡©æº¶æ¶²ïŒPH7.2ïŒã«åæžæ¿ãã
ïŒã¿ãŒã²ãã詊è¬ã詊éšããããã®æšæºæ³ãåç §
ããããä»ã®ã¿ãŒã²ãã補å€ã«ããâTAGã
ãã³ïŒŠâTAGãå«ãããšã詊éšã«ããæãããš
ãªã€ãå®¶å å šè¡æž ãåŸãããšãã§ãããªãã°ãåŸ
èšæ¹æ³ã«ããäººè¡æž ã®åå¿ãã粟補ããæšæºç
âTAGããã³ïŒŠâTAGã䜿çšããããšãã§ã
ãïŒã æ¬¡ã®æ¹æ³ã«ããã¹ããŒâãã€ã³ãã³ã°ïŒïŒ³â
TAGïŒã決å®ããïŒæ°æ¥éä¿åããåçµè¡æž ã¯
ããã䜿çšãã¹ãã§ãªããåžžå¥ã®æ¹æ³ã«ãããæ°
ããåŸãããå šè¡æ¶²ãããã¯ä»ã®äœæ¶²ããæ³šæã
ãªããè¡æž ãã€ãããæºè¶³ãã¹ãæ¹æ³ãè¿°ã¹ãã°
次ã®ãšããã§ãããæ¡åããè¡æ¶²ãã¬ã©ã¹è£œè©Šéš
管äžã宀枩ã§ïŒæéæŸçœ®ããåè¡ããããã¬ã©ã¹
è£œæ¹ææ£ãçšããåè¡ã詊éšç®¡å£ããåé¢ããã
ã®è¡æ¶²ãïŒâã§æäœïŒæéïŒãããã¯äžå€ïŒæŸçœ®
ãããïŒâã§45åéé å¿åé¢ïŒ20000rpmïŒãã
ããšã«ããåè¡ãè¡æž ããåé¢ããè¡æž ãé å¿å
é¢ç®¡äžã«åŸçåé¢ããæŽã«ïŒâã§45åéé å¿åé¢
ïŒ2000rpmïŒããããã®è¡æž ãåŸçããã¡ããªã¬
ãŒãïŒïŒ æº¶æ¶²ïŒæ°Ž95mlããã³0.2Mçé žæ°ŽçŽ å¡©ç·©
è¡æ¶²ïŒPH10ïŒïŒmläžãïŒïœïŒãè¡æž ã«å¯ŸããŠïŒïŒ
ïŒå®¹éïŒã®é床ã§å ããã äžè𿹿³ãŸãã¯ä»ã®æ¹æ³ã§åŸãããè¡æž ããã
ã0.2mlããã¿ãŒã²ãã詊è¬0.25mlåœãã¬ã³ã°ã
ã³100ã200mg嫿ã®ã¿ãŒã²ããæžæ¿æ¶²è©Šè¬ããã
ã0.25mlã«å ããïŒåã®æ€æ»è©Šæãšããããã¬ã
ãçæãé¿ãããããããªæ¹æ³ã«ãããäžèšæžæ¿
æ¶²ãïŒâã§æ··åãããããšãã°å°ãããŽã 補ãã€
ãããçšããŠïŒãïŒç§éåŒ·ãæ¯ããŸããåŸã管ã
ããããã«åŸçãããŠãããããŒãã¹ã·ãšãŒã«
äžãçŽïŒæéãŸãã¯ããä»¥äžæ¯çªããããã¿ãŒã²
ãã詊è¬ãšããã«çµåããèçœãè¡æž ããåé¢ã
ãããã®çºæã§èŠåºãããæºè¶³ãã¹ãæ¹æ³ã«ã
ããäžèšç®¡ãïŒâã§20åéé æ²ïŒ2000rpmïŒãäž
æŸãåŸçããåŸãããã0.15Mé£å¡©æ°Ž0.2ã0.3ml
ãšåæ··åããå®€æž©ã§æ¯çªããé æ²ããŠäžæŸæ¶²ãå
é¢ããæäœãïŒåè¡ãããšã«ããçæãããã¬ã
ããæŽæµããã ã¿ãŒã²ããã«ä»çããŠæ®çããèçœããããã
åé¢ãããã®èçœãå®éãããããšãã°0.25Mé ¢
é ž0.2mlãå ãããã®æžæ¿æ¶²ã37âã®ãµåµåšäžã§
ïŒãïŒæéæ¯ããŸããã管ãïŒâã§30åéé æ²
ïŒ2000rpmïŒãããç²ç¶ç©ãç§»è¡ããã®ãé¿ãã
ããã«æ³šæããªããäžæŸæ¶²ãåŸçãã280ïœÎŒã«
ãããäžæŸæ¶²ã®å åŠå¯åºŠã枬å®ãããè¡æž èçœ
ïŒïŒ³âTAGïŒmlåœãmgã®çµæãåŸããããäžèšå
åŠå¯åºŠã®æž¬å®å€ã1.46ã®å åã§å²ããæ€æ»è³æïŒ
åã®éã«ïŒïŒ 以äžã®å·®ããã€ãŠã¯ãªããªããèçœ
å«éãæ€æ»ãããããä»ã®æ¹æ³ããšãã°ããªãªã³
âããŠãªã€æ€æ»æ³ãçšããããšãã§ããããå¯Ÿç §
詊æã®æ¿åºŠãšïŒïŒ³âTAGïŒâïŒïŒŠâTAGïŒæ¿åºŠã®
å€ã®ç¯å²ãæšæºæ³ã«ãããŠç¹å®ããŠãããã°ãªã
ãªãã ãã¢ã¹ãâãã€ã³ãã³ã°ïŒïŒŠâTAGïŒå€ã¯æ¬¡
ã®ããã«æ€æ»ããïŒæ°æ¥ä»¥äžåçµããŠä¿åããã
è¡æž ã¯äœ¿çšãã¹ãã§ã¯ãªããåžžå¥ã®æ¹æ³ã«ããã
æ°ããåŸãããå šè¡æ¶²ãããã¯ãã®ä»ã®äœæ¶²ãã
泚æããŠè¡æž ãäœæãããè¡æž ãã€ããæ¹æ³ã¯ã
ã®å®æœäŸã®åéšã«èšèŒããæ¹æ³ã§å åã§ããã äžèšã®åã¯ä»ã®ææ³ã§äœæãããè¡æž èŠæ¬ã¯ã
âTAGè¡æž 枬å®å€ãäžèšã®TARGET詊å€ãš
æ¥è§Šãããããå šæéããã10åéå°ãªãæéã®
éãïŒâã§æŸçœ®ããïŒäŸãã°ãïŒæéãã®ïŒ³â
TAG枬å®ããããªãã°ïŒæé50åïŒããã®æ¹æ³ã§
ã¯ïŒ³âTAGãšïŒŠâTAGã®æž¬å®å€ã®æž©åºŠå±¥æŽãå¹³
è¡¡ãããã æž©åºŠå¹³è¡¡ããè¡æž ã®èŠæ¬0.2mlããäºé枬å®ã«
ãããŠãTARGET詊å€0.25mlã«ã€ã
RECOGNIN100ä¹è³200ãã€ã¯ãã°ã©ã ã嫿ã
ãTARGETããæ¿è©Šå€ã®0.25mlæŽé€æ°ã®ããã
ãã«å ãããããæ¿ç©ã¯ãé¡ç²åœ¢æãé¿ãããã
ã«ããŠçŽ10åéïŒâã§æ··åãããäŸãã°ãæ¥éã«
æ¯ãåãããå°ãŽã ãã€ãããïŒãïŒç§éçšã
ãŠããããã管ãå ãã«åŸããŠããããããŒãã¹
æ¯ãšãåšã§çŽ10å鿝ãšãããã°ãããããã«ã€
ããŠããTARGET詊å€åã³èçœè³ªã¯è¡æž ããå
é¢ããããæºè¶³ã§ãããšèªããããææ³ã®äžã€ã¯
äžèšã®ãã®ã§ãããæ¬¡ã«ç®¡ã2000rpmã§20åéïŒ
âã§é å¿åé¢ããäžæŸæ¶²ãéæ³šããé å¿åé¢ã§åœ¢
æããé¡ç²ã0.15Må¡©æ°Ž0.2ã0.3mlãšåæ··åã宀
æž©ã§æ¯ãšãããããšã«ãã€ãŠïŒåæŽæµããé å¿å
é¢ããŠãäžæŸæ¶²ã廿£ããã æšçã«ä»çãããŸãã«ãªã€ãŠããèçœè³ªããã
ããéè£ããå®éçã«æž¬å®ãããâTAGæ¿åºŠ
ãæž¬å®ããããã®æ¬èŠæ¬ã«ãããäžèšã®ææ³ã¯æº
è¶³ãªãã®ã§ãããããªãªã³ã»ããŠãªãŒæž¬å®æ³ã®ã
ããªãèçœè³ªå«æéãæž¬å®ããã®ã«å¹æã®ããä»
ã®ææ³ãçšããŠãããããããå¶åŸ¡ã®ç¯å²åã³ïŒ³
âTAGãã€ãã¹ïŒŠâTAGæ¿åºŠã®è «çå€ã決å®ã
ãããåºæºãå®ããªããã°ãªããªãã æçµçµæã¯è¡æž ïŒmlã«ã€ãTAGãã€ã¯ãã°ã©
ã ãšããŠè¡šããããããã¯ïŒ³âTAGãã€ãã¹ïŒŠ
âTAGã«çãããéè³è «çæ£è åã³ä»ã®å¶åŸ¡ã«
ãããTAGå€ã¯çŸåšã®ãšããè¡æž ïŒmlã«ã€ããŒ
ãïŒåã¯è² æ°ïŒãã135ãã€ã¯ãã°ã©ã ãŸã§ã®ç¯
å²ã«äºããïŒmlã«ã€ã100ïœÎŒãè¶ããçµæã«ã€
ããŠã¯ãåèŠæž¬å®ã衚瀺ãããä»ã®è «çã®ããã
ã®ã¯é«ãTAGå€ã衚ããããšããããç¹ã«è³å
ã«äºæ¬¡çïŒè»¢ç§»ããïŒè «çãããå Žåã«ããã§ã
ããè³è «çæ£è ã®TAGå€ã¯çŸåšã§ã¯è¡æž ïŒmlã«
ã€ã136ä¹è³500以äžãã€ã¯ãã°ã©ã ã«åã¶ãã¢ã¹
ããã·ãã³åã³ããã¢ã¢ã»ãã«ã»ã«ããŒãºãã調
æŽããTARGET詊å€ãå©çšããæ¬å®æœäŸã®ææ³
ã«åŸã€ãŠè¡ãªã€ã50ã®è¡æ¶²èŠæ¬ã«ã€ããŠã®æåã®
ãç²æ€ãã«ãããŠã¯ã11ã®è³è «çã®ãã¡11ãåã³
32ã®æ£åžžã®ãã®ããã¡28ãæ£ç¢ºã«å€å¥ããããïŒ
ä»¶ã®æ£åžžãšæãããŠãããã®ïŒå³ã¡ãéè³è «çå¶
埡ïŒã®ãã¡ïŒä»¶ã¯ãæããã«äœå¹Žãåã«è¯ãæåœ
ããç²ç¶è ºçã®ããããšã倿ãããæ®ãã®ïŒä»¶
ã®æ£åžžè ã¯å¥åº·ã®ããããªããå€å蚺æã®ã€ããŠ
ããªãçãæã€ãã幎什60ã70ã®å人ã§ãã€ãã
æ®ãã®ïŒäŸã®ãã¡ãããžãã³ã¹ç ã®ïŒä»¶äžã®ïŒä»¶
ã¯æ£ç¢ºã«å€å¥ããããè «çã®ç¯å²ã«å ¥ãïŒäŸ
ïŒ136ã500ÎŒïœTAGïŒmlïŒã¯ã²ã©ãç¥çµè çãæ
ã€æ£è ã«è©²åœããéè «çã®ç¯å²ïŒïŒã135ÎŒïœ
TAGïŒmlïŒã«å ¥ãïŒäŸã¯ãããããäžæç¢ºã§ã¯
ãããè «çã§ã¯ãªãé èå å¡ã®èšºæãåã³éªšèè «
ïŒéè³è «çïŒåã³é»ç®èè «ïŒéè³è «çïŒãæã€ã
æ£è ã«çžåœããã MALIGNINåã³æªæ§è³è «çåã³å šæ£åžžè ãã
調補ããTARGET詊å€ãå©çšããŠæ¬å®æœäŸã®æ
æ³ã«åŸã€ãŠãã®åŸã®æ€èšãè¡ãªã€ãã æ¬å®æœäŸã®ææ³ã«åŸã€ãŠè¡ãªã€ãæŽã«ãã以äž
ã®æ€èšã§ã¯è©Šéšãã人éã®è «çã®èŠæ¬ã®ç·æ°ã50
ãã114ã«åãã ããããã®è£œååã³ææ³ã®æçš
æ§ã第ïŒè¡šã«ç€ºãããããã¯ãããã®è©Šéšã®çµæ
ãèšé²ãããã®ã§ããã 宿œäŸ ïŒ ã€ã ããã«ãªã¬ã³ã»ã³ã¹ã«ããè «ç现èã®èšºæ ååç©æâã¢ã¹ããã·ãã³ãæâããªã°ãã³ã
ãã³ïŒ³âTAGã¯è «ç现èã«åªå çã«ä»çããã
ãšã瀺ãããã®ç¹ç°æ§ã¯çµç¹åŠã®éšéã«ãããŠæ
æãŸãã¯æŸå°æ§ç©è³ªãæâã¢ã¹ããã·ãã³ãæâ
ããªã°ãã³ãŸãã¯ïŒ³âTAGã«çµåãããŠããã
ã®ååç©ãè «ç现èã®èšºæã«çšãåŸããæšæºã®æš
èæè¡ãããããçšãããããâTAGã®äœ¿çš
æ³ã¯æ¬¡ã®ãšããã§ããã æºè¶³ã§ãããšèªããããäžã€ã®æ¹æ³ã¯ã»ã³ãã»
ããªãŒïŒSt.MarieïŒæ³ã®å€åœ¢ã§ããã人éã®è³
è «çæ€äœãåçµãïŒãã¯ãã³ã®åãã«åãããã
ããæ¹¿å®¹åšäžã«æè²åã«â70âã«ïŒãïŒé±é貯ã
ããçµåäœã¯å±±çŸã®æâãŠãµã®çµåäœã®ãããªæš
æºã®æè¡æž ã§ãããçµåäœã¯ãã«ãªã¬ãã»ã³ããŸ
ãã¯ä»ã®æšæºç©è³ªã§ãã®æè¡åéã§æ¢ç¥ã®ææ³ã«
ãã€ãŠæšèããããåžè²©ã®ãã«ããšãã»ã€ã³æšè
å±±çŸæâãŠãµã®çµåäœãçšãåŸããçšãããã«ãª
ã¬ãã»ã³ãææ³ã¯æšæºã®ãã®ã§ãã€ãŠãTAGã®
ïŒïŒ200ãïŒïŒ400溶液ãè «çåçäžã«30åéãŸã
ã¯ãã以äžä¿æž©ããæ¬¡ã«æŽæµããŠä»çããªã
TAGãé€ããçé žç·©è¡é£å¡©æ°Žã§ïŒåæŽæµããã®
ãããããã«ãªã¬ãã»ã€ã³âæšèçµåäœã§çµåä¿
æž©ããæŽæµãããé¡åŸ®é¡æ€æ»ããããæ£èŠã®çްè
ããã³ãã®çªèµ·ã¯è «çåçããã³æ£èŠã®éè «çè³
ã®å¯Ÿç §åçå ±ã«æè²ãããªãããã«ãªã¬ãã»ã³ã¹
ã¯è «çç¥çµè ããã³ãã®çªèµ·ã«æããååšããã 宿œäŸ 10 çµç¹å¹é€ã§å¢æ®ããè «ç现èã«å¯ŸããŠæâã¢ã¹
ããã·ãã³ãæâããªã°ãã³ããã³ïŒ³âTAG
ãçŽ°èæ¯æ§ãæããããšã®å®éš çŽ°èæ¯æ§ã枬å®ããæšæºè©Šéšãçšãããäžè¬
ã«ãåºå®ããèšç®ç®±äžã®çްèã®æ°ãæ®éçŽ100ã®
çãã现èã嫿ããããã«æºåããããããã
ãèšç®ãããæ¬¡ã«ãããã®çްèã詊éšãã詊å€ã§
åŠçãããªãçããŠãã现èã®æ°ãèšç®ããã 宿œäŸïŒã®æ¹æ³ã§åŸãããâTAG溶液ã®ã
ç¥çµè çºçãååã«ç¹åŸŽã¥ãããããç¥çµè èœçް
èãçŽã®æ£è ããã®çµç¹å¹é€äžã®çްèã«å¯Ÿã
ãçŽ°èæ¯æ§ã®æšæºè©Šéšã«ãããŠãâTAGã¯æº¶
æ¶²mlåœãâTAG0.2ããã³0.02ÎŒïœã®ãããªå°é
ã«çžåœããïŒïŒ100ããã³ïŒïŒ1000ã®é«åžé床ã®
å Žåã§ãèšç®ç®±äžã®ãã¹ãŠã®çްèãæ»æ» ãããã
åæ§ãªçµæãæâã¢ã¹ããã·ãã³ããã³æâããª
ã°ãã³ã®é«åžéæ¶²ã§åŸãããã 宿œäŸïŒã§ç€ºãç¹ç°æ§ããã³å®æœäŸ10ã§ç€ºã现
èæ¯æ§ã¯å ±ã«äººéã®è³è «çã«å¯Ÿããæâã¢ã¹ãã
ã·ãã³ãæâããªã°ãã³ããã³ïŒ³âTAGã®æ²»ç
å¯èœæ§ã«éåžžã«é¢ä¿ãããããããã®æ²»çç䜿çš
ã¯æè¡ã§é€å»ãããè «ççµç¹ã«å¯Ÿãããããã®äž¡
æ¹ã®çŸè±¡ã®å®éã®èšºæå¯èœæ§ã«å ããŠæ¢ã«èšŒæã
ãçµç¹åŠã«ãã蚺æãå¿ èŠãšããã
ã衚ã
ã衚ã
宿œäŸ 11
ã¬ã³ã°ãã³ã®å æ°Žéè£
ã¬ã³ã°ãã³ã®æº¶æ¶²ããã®å Žåã¯ã¢ã¹ããã·ãã³
ãããªã°ãã³ãPHïŒãïŒã§å·çœ®ãããïŒã14æ¥åŸ
ã«èå±€ã¯ãããã°ã©ãã€ãŒã§ãã®çæç©ã¯ååé
ãçŽ200æžå°ããããšã瀺ãããã®æº¶æ¶²ãæŽã«é·
ãæŸçœ®ãããšçŽ200ååéã®åäœãïŒã10æ¥ã«é
è£ãããããªãã¡ã¢ã¹ããã·ãã³ã§ã¯8000ããå
åéãæžå°ããããªã°ãã³ã§ã¯10000ããååé
ãæžå°ããåå ŽååŒç¶ããŠçŽ200ã®åäœæžå°ããã ã¢ã¹ããã·ãã³ã®ççååŠçç¹ç°æ§ã¯åçæç©
ã§4000ååéãŸã§ç¶æããããããªã°ãã³ã®çç
ååŠçç¹ç°æ§ã¯åçæç©ã§5000ååéãŸã§ç¶æã
ãããããã¯æâã¢ã¹ããã·ãã³ããã³æâããª
ã°ãã³ã®ããããã«å¯ŸãããªãŠã¯ãã«ãããŒã»ã²
ã«æ¡æ£è©Šéšã§ç€ºãããã ãã®éè£ã¯ãŸãããªãã·ã³ããã³ãã®ä»ã®ãã
ãã¢ãŒãŒãšå ±ã®ããã«é µçŽ çã«ãåãçµæã§éæ
ããããšãã§ããã ã¬ã³ã°ãã³ã®å æ°Žéè£ã«ãã€ãŠèª¿è£œããããã
ãã®ååç©ã®ååéã¯æ¬¡åŒã«ãã€ãŠã»ãŒå®çŸ©ãã
ãã ã¢ã¹ããã·ãã³ã®ççååŠçç¹ç°æ§ãæããç
æç©ã«å¯ŸããŠã¯ïŒ400ïŒ200XïŒïŒ¹ ããªã°ãã³ã®ççååŠçç¹ç°æ§ãæããçæç©
ã«å¯ŸããŠã¯ïŒ5000ïŒ200XïŒïŒ¹ ããã«ïŒ¹ã¯çæç©ã®ååéã§ïŒžã¯ïŒãïŒã®æŽæ°
ã§ããã 宿œäŸ 12 ã¬ã³ã°ãã³ãšã®äººå·¥çµç¹ãŸãã¯åšç®¡ã®è£œé ãã©ã¹ããã¯ãéå±ãŸãã¯ãã®ä»ã®é©åœãªãã
ãææã®ãããå£ã®ç®¡ãã¬ã³ã°ãã³ïŒãã®å Žåã¢
ã¹ããã·ãã³ãŸãã¯ããªã°ãã³ïŒã®é«æ¿åºŠïŒå³ã¡
10mgïŒmlïŒã®ãŽã€ã¹ã³ãŒã¹æº¶æ¶²ãããã¹ãŠã®è¡šé¢
ã被èŠããããŸã§æµžããå«ãŸããããããã¯ãŸã
ã¬ã³ã°ãã³æº¶æ¶²ããã¹ãŠã®è¡šé¢ãååã«è¢«èŠãã
ããŸã§å å§äžã«ç®¡ã®äžããã³åšãéããæ¬¡ã«ç®¡ã
颚也ãŸãã¯æžå§äžã«ä¹Ÿç¥ãããåçµä¹Ÿç¥ããã被
èŠæäœãã¬ã³ã°ãã³ã®å€ãã®ååéã®è¢«èŠãã§ã
ãããã«æ°åç¹°è¿ãã ãã®ç®¡ã¯çããŠããåºä¹³åç©ã®é£æ¥ããçµç¹ãŸ
ãã¯äœæ¶²äžã«ã¢ã¹ããã·ãã³ãŸãã¯ããªã°ãã³ã®
ãããªåé§ç©è³ªã嫿ãã空æŽãŸãã¯çµç¹äžã«çœ®
ãæºåãããããŠã§ããããã®äººå·¥çµç¹ãŸãã¯åš
å®ã¯ã¬ã³ã°ãã³è¢«èŠã®ãªãç°çš®ç©è³ªãåºæ¿ããã
åå¿ãæå°ã«ãããé€ãããã«çšããããã ãã®ä»ã®åœ¢ç¶ã®äººå·¥çµç¹ãŸãã¯åšå®ãåæ§ã«ã
補ããããšãã§ããã ä»¥äžæ¬çºæã®æè¡çç¯å²ã«å å«ãããå ·äœçæ
æ§ãèšèŒããã ïŒ è³è è «è «ççµç¹ãäžæ§ç·©è¡æ¶²ã§æœåºããŠãè
çœè³ªãã©ã¯ã·ãšã³ã坿º¶åãããåŸããã坿º¶
åèçœè³ªåºæ¶²ããpKå€çŽïŒãïŒã®ãã©ã¯ã·ãš
ã³ãåé¢ããããããååéçŽ8000ã®ç©è³ªãå
é¢ããããšãç¹åŸŽãšããã¢ã¹ããã·ãã³ã®è£œé
æ³ã ïŒ pKå€çŽïŒãïŒã®ãã©ã¯ã·ãšã³åé¢å·¥çšãã
坿º¶æ§èçœè³ªæœåºç©ãã¯ãããã°ã©ãã€ãŒçšã«
ã©ã ã«å ããåŸã ã«é žæ§åºŠãäžããæº¶å€ã§æº¶é¢
ãããããšã«ããéè¡ããåèšïŒèšèŒã®æ¹æ³ã ïŒ æº¶é¢æ¶²ã溶åºãããŠã¢ã¹ããã·ãã³å«æãã©
ã¯ã·ãšã³ãåŸããããèå±€ã¯ãããã°ã©ãã€ãŒ
ã§åé¢ããããšã«ããåé¢ãè¡ãåèšïŒèšèŒã®
æ¹æ³ã ïŒ å®éæ²é詊éšããã³å¯å€©å æåæäœã²ã«åæ£
詊éšã«ãããŠãã®ç¹ç°çæäœã§åäžç·ç¶æ²æŸ±ã
圢æãããã€é žæ§ãŸãã¯äžæ§ã®æ°ŽãŸãã¯æ°Žæ§æº¶
æ¶²ã«æº¶è§£ãã¢ã«ã«ãªæ°Žã«ã¯æº¶è§£ããªãåå åžå
æ³¢é·ããŒã¯280ïœÎŒãååéçŽ8000ã®åèšïŒèš
èŒã®æ¹æ³ã§è£œé ããã¢ã¹ããã·ãã³ã ïŒ ã¢ã¹ããã·ãã³ ïŒ å®éæ²æŸ±è©Šéšããã³å¯å€©å æåæäœã²ã«åæ£
詊éšã«ãããŠãã®ç¹ç°çæäœã§æ²æŸ±ã圢æãã
ãã€é žæ§ãŸãã¯äžæ§ã®æ°ŽãŸãã¯æ°Žæ§æº¶æ¶²ã«æº¶è§£
ãã¢ã«ã«ãªã«ã¯æº¶è§£ããªãåå åžåæ³¢é·ããŒã¯
280ïœÎŒãååéçŽ8000ã®åèšïŒèšèŒã®çæç©ã ïŒ ã¢ããé žçµæç©ãå€§ç¥æ¬¡ã®åŠã嫿ãã æ®åºæŠç¥æ° ã¢ã¹ãã©ã®ã³é ž ïŒ ã¹ã¬ãªãã³ ïŒ ãŒãªã³ ïŒ ã°ã«ã¿ãã³é ž 13 ãããªã³ ïŒ ã°ãªã·ã³ ïŒ ã¢ã©ãã³ ïŒ ããªã³ ïŒ 1/2ã·ã¹ãã³ ïŒ ã¡ããªãã³ ïŒ ã€ãœãã€ã·ã³ ïŒ ãã€ã·ã³ ïŒ ããã·ã³ ïŒ ããšãã«ã¢ã©ãã³ ïŒ ãªãžã³ ïŒ ãã¹ããžã³ ïŒ ã¢ã«ã®ãã³ ïŒ åèšïŒæŠç¥ïŒ 88 ãã€ãã¢ããé žã·ã¹ãã³é žãããããã·ãã
ãªã³ããã«ãã€ã·ã³ãã¢ã³ã¢ãã¢ãã€ãœãã¹ã¢
ã·ã³ããã¹ã¢ã·ã³ãããããã·ãªãžã³ããªãžã
ãã«ãã€ã·ã³ããã³Î³âã¢ããé ªé žãæ€åºéã¯
ååšããªãåèšïŒèšèŒã®çæç©ã ïŒ ããã¢ã¢ã»ãã«ã»ã«ããŒã¹ãšè€åäœã圢æ
ããããã¢ã¢ã»ãã«âã»ã«ããŒã¹âã¢ã¹ããã·
ãã³ãšãªãããã€åºä¹³åç©ã«æäžããŠç¹ç°çæ
äœã¢ã³ãâã¢ã¹ãããã³ãçæãã該ã¢ã³ãâ
ã¢ã¹ãããã³ãã€ã³ã»ãããã«ãããŠè³è «çã«
ææ¯ã§ãã€ãŠããã«ãªã¬ãã»ã³ãšçµåãããšè
è «çŽ°èã®è¢å ãçããæ§è³ªãæããåèšïŒèšèŒ
ã®çæç©ã ïŒ ã¢ã³ãâã¢ã¹ãããã³ãããã¢ã¢ã»ãã«ã»ã«
ããŒã¹âã¢ã¹ããã·ãã³ãããã¢ã¢ã»ãã«âã»
ã«ããŒã¹âã¢ã¹ãã°ãã·ãã³âã¢ã³ãâã¢ã¹ã
ãã·ãã³ãã¢ã³ãâããªã°ãã³ãããã¢ã¢ã»ã
ã«ã»ã«ããŒã¹âããªã°ãã³ããã³ããã¢ã¢ã»ã
ã«âã»ã«ããŒã¹âããªã°ãã³âã¢ã³ãâããªã°
ãã³ããªãã矀ããéžã°ããäžçš®ã 10 å¹å°å ã§çè²ãã人工ç现èãäžæ§ç·©è¡æ¶²ã§
æœåºããŠèçœè³ªãã©ã¯ã·ãšã³ã坿º¶åãããåŸ
ãããæœåºæ¶²ããpKå€çŽïŒãïŒã®ãã©ã¯ã·ãš
ã³ãåé¢ããããããååéçŽ10000ã®ç©è³ªã
åé¢ããããšãç¹åŸŽãšããããªã°ãã³ã®è£œé
æ³ã 11 pKå€çŽïŒãïŒã®ãã©ã¯ã·ãšã³ãåé¢ããå·¥
çšãã坿º¶æ§èçœè³ªã®è©²æœåºç©ãã¯ãããã°ã©
ãã€ãŒçšã«ã©ã ã«å ããæŒžæ¬¡é žæ§åºŠããããæº¶
å€ã§æº¶é¢ãããããšã«ããéè¡ããåèš10èšèŒ
ã®æ¹æ³ã 12 溶é¢åºŠã溶åºãããŠããªã°ãã³å«æãã©ã¯ã·
ãšã³ãåŸããããèå±€ã¯ãããã°ã©ãã€ãŒã§å
é¢ããããšã«ããåé¢ãè¡ãåèš11èšèŒã®æ¹
æ³ã 13 å®éæ²é詊éšããã³å¯å€©å æåæäœã²ã«åæ£
詊éšã«ãããŠãã®ç¹ç°çæäœã§åäžç·ç¶æ²æŸ±ã
圢æãããã€é žæ§ãŸãã¯äžæ§ã®æ°ŽãŸãã¯æ°Žæ§æº¶
æ¶²ã«æº¶è§£ãã¢ã«ã«ãªæ°Žã«ã¯æº¶è§£ããªãåå åžå
æ³¢é·ããŒã¯280ïœÎŒãååéçŽ10000ã®åèš10èš
èŒã®æ¹æ³ã§è£œé ããã¢ã¹ããã·ãã³ã 14 ããªã°ãã³ 15 å®éæ²æŸ±è©Šéšããã³å¯å€©å æåæäœã²ã«åæ£
詊éšã«ãããŠãã®ç¹ç°çæäœã§æ²æŸ±ã圢æãã
ãã€é žæ§ãŸãã¯äžæ§ã®æ°ŽãŸãã¯æ°Žæ§æº¶æ¶²ã«æº¶è§£
ãã¢ã«ã«ãªã«ã¯æº¶è§£ããªãåå åžåæ³¢é·ããŒã¯
280ïœÎŒãååéçŽ10000ã®åèš14èšèŒã®çæ
ç©ã 16 ã¢ããé žçµæç©ãå€§ç¥æ¬¡ã®åŠã嫿ãã æ®åºæŠç¥æ° ã¢ã¹ãã©ã®ã³é ž ïŒ ã¹ã¬ãªãã³ ïŒ ãŒãªã³ ïŒ ã°ã«ã¿ãã³é ž 13 ãããªã³ ïŒ ã°ãªã·ã³ ïŒ ã¢ã©ãã³ ïŒ ããªã³ ïŒ 1/2ã·ã¹ãã³ ïŒ ã¡ããªãã³ ïŒ ã€ãœãã€ã·ã³ ïŒ ãã€ã·ã³ ïŒ ããã·ã³ ïŒ ããšãã«ã¢ã©ãã³ ïŒ ãªãžã³ ïŒ ãã¹ããžã³ ïŒ ã¢ã«ã®ãã³ ïŒ åèšïŒæŠç¥ïŒ 89 ãã€ãã¢ããé žã·ã¹ãã³é žãããããã·ãã
ãªã³ããã«ãã€ã·ã³ãã¢ã³ã¢ãã¢ãã€ã¹ãã¹ã¢
ã·ã³ããã¹ã¢ã·ã³ãããããã·ã«ã·ã³ããªãžã
ãã«ãã€ã·ã³ããã³Î³âã¢ããé ªé žãæ€åºéã¯
ååšããªãåèš15èšèŒã®çæç©ã 17 ããã¢ã¢ã»ãã«ã»ã«ããŒã¹ãšè€åäœã圢æ
ããããã¢ã¢ã»ãã«âã»ã«ããŒã¹âããªã°ãã³
ãšãªãããã€åºä¹³åç©ã«æäžããŠç¹ç°çæäœã¢
ã³ãâããªã°ãã³ãçæãã該ã¢ã³ãâããªã°
ãã³ãã€ã³ã»ãããã«ãããŠè³è «çã«ææ¯ã§ã
ã€ãŠããã«ãªã¬ãã»ã³ãšçµåãããšè è «çŽ°èã®
è¢å ãçããæ§è³ªãæããåèšïŒèšèŒã®çæ
ç©ã 18 è¡æž ãŸãã¯ä»ã®äœæ¶²ãããã¢ã¢ã»ãã«ã»ã«ã
ãŒã¹âã¢ã¹ããã·ãã³ãŸãã¯ããã¢ã¢ã»ãã«ã»
ã«ããŒã¹âããªã°ãã³ãšåè§£ã®èµ·ãã¬äœæž©ã§çŽ
ïŒæéãŸãã¯ãã以äžåå¿ãããåŸãããç©è³ª
ãåžé žã§åŠçããŠã¹ããŠâã¿ãŒã²ããä»å ã°ã
ããªã³ãè§£é¢ããããããšãç¹åŸŽãšããã¹ããŠ
âã¿ãŒã²ããä»å ã°ãããªã³ã®è£œé æ³ã 19 ç·©è¡æ°Žæº¶æ¶²ã«æº¶è§£ããå¯å€©å æåæäœã²ã«å
æ£è©Šéšã«ãããŠã¢ã¹ããã·ãã³ãŸãã¯ããªã°ã
ã³ã§åäžç·ç¶æ²æŸ±ã圢æããã»ããã¢ã³èã§é
æãããååé25000以äžã®ååãä¿æããã
ãªãã¢ã»ãã€ã«ã¿ãŒã«ãã€ãŠä¿æãããèå±€ã²
ã«ã¯ãããã°ã©ãã€ãŒã«ããæž¬å®ãããååé
ãåéäœã®ç¶æ ã«ããçŽ50000ããã³ãã¯ãã°
ãããªã³ç¯å²ã«å°ããã®æ°åã®ååéãæãã
åå åžåæ³¢é·ããŒã¯280ïœÎŒãæããåèš18èš
èŒã®æ¹æ³ã§èª¿è£œããã¹ããŠâã¿ãŒã²ããä»å ã°
ãããªã³ã 20 è¡æž ãŸãã¯ä»ã®äœæ¶²ãããã¢ã¢ã»ãã«ã»ã«ã
ãŒã¹âã¢ã¹ããã·ãã³ãŸãã¯ããã¢ã¢ã»ãã«ã»
ã«ããŒã¹âããªã°ãã³ãšåè§£åå¿ã®èµ·ãã¬äœæž©
ã§çŽ10åéåå¿ãããåŸãããç©è³ªãçšé žã§åŠ
çããŠãã¢ã¹ãã¿ãŒã²ããä»å ã°ãããªã³ãåŸ
ãããšãç¹åŸŽãšãããã¢ã¹ãã¿ãŒã²ããä»å ã°
ãããªã³ã®è£œé æ³ã 21 ç·©è¡æ°Žæº¶æ¶²ã«æº¶è§£ããå¯å€©å æåæäœã²ã«å
æ£è©Šéšã«ãããŠã¢ã¹ããã·ãã³ãŸãã¯ããªã°ã
ã³ã§åäžç·ç¶æ²æŸ±ã圢æããã»ããã¢ã³èã§é
æãããååé25000以äžã®ååãä¿æããã
ãªãã¢ã»ãã€ã«ã¿ãŒã«ãã€ãŠä¿æãããèå±€ã²
ã«ã¯ãããã°ã©ãã€ãŒã«ããæž¬å®ãããååé
ãåéäœã®ç¶æ ã«ããçŽ50000ããã³ãã¯ãã°
ãããªã³ç¯å²ã«å°ããã®æ°åã®ååéãæãã
åå åžåæ³¢é·ããŒã¯280ïœÎŒãæããåèš20èš
èŒã®æ¹æ³ã§èª¿è£œããã¹ããŠâã¿ãŒã²ããä»å ã°
ãããªã³ã 22 補é ãããã¹ããŠâã¿ãŒã²ããä»å ã°ãããª
ã³ããã³ãã¢ã¹ãâã¿ãŒã²ããä»å ã°ãããªã³
ã®æ¿åºŠãå ¬ç¥éã®åºä¹³åç©ã®è¡æž ãŸãã¯äœæ¶²ã«
ãã€ãŠæž¬å®ããããšãç¹åŸŽãšããåºä¹³åç©äžã®
çè «çã®æ€åºæ³ã 23 ã¢ã¹ããã·ãã³ã®ç©çååŠçç¹æ§ãæããæ§
é ããæ±ºå®ãããååéãçŽïŒ4000ïŒ200XïŒ
ïŒïŒåŒäžãã¯çæç©ã®ååéãã¯ïŒã19
ã®æŽæ°ïŒãæããçæç©ã 24 ããªã°ãã³ã®ç©çååŠçç¹æ§ãæããæ§é ã
ãæ±ºå®ãããååéãçŽïŒ4000ïŒ200XïŒïŒ¹ïŒ
ïŒåŒäžãã¯çæç©ã®ååéãã¯ïŒã19ã®æŽ
æ°ïŒãæããçæç©ã 25 ã¢ã¹ããã·ãã³ã«å¯Ÿå¿ããæäœãåºä¹³åç©äž
ã«çããããããšãç¹åŸŽãšããæã¢ã¹ããã·ã
ã³ã®è£œé æ³ã 26 ã¢ã¹ããã·ãã³ããã³ããªã°ãã³ãããªã矀
ããéžã°ããã¬ã³ã°ãã³ãšããã¢ã¢ã»ãã«ã»ã«
ããŒã¹ãåºä¹³åç©äžã§æ··åãè€åäœã圢æãã
ãããšãç¹åŸŽãšããããã¢ã¢ã»ãã«ã»ã«ããŒã¹
âã¬ã³ã°ãã³ã®è£œé æ³ã 27 ã¢ã³ãã¬ã³ã°ãã³ãšã¬ã³ã°ãã³ãã¢ã¹ããã·
ãã³ããã³ããªã°ãã³ãããªã矀ããéžã°ãã
ããã¢ã¢ã»ãã«ã»ã«ããŒã¹âã¬ã³ã°ãã³ãåºä¹³
åç©äžã§åå¿ããè€åäœã圢æãããããšãç¹
城ãšããããã¢ã¢ã»ãã«ã»ã«ããŒã¹âã¬ã³ã°ã
ã³âã¢ã³ãâã¬ã³ã°ãã³ã®è£œé æ³ã 28 ã¬ã³ã°ãã³ãã¢ã¹ããã·ãã³ããã³ããªã°ã
ã³ãããªã矀ããéžã°ããããã¢ã¢ã»ãã«ã»ã«
ããŒã¹âã¬ã³ã°ãã³âã¢ã³ãâã¬ã³ã°ãã³ãé ž
ãŸãã¯ãããããŒãŒé µçŽ ã«ããå æ°Žåè§£ããã
ãšãç¹åŸŽãšããã¢ã³ãâã¬ã³ã°ãã³ã®è£œé æ³ã 29 ãã«ã®ãã³ã«å¯Ÿå¿ããæäœãåºä¹³åç©ã«æäž
ããããšãç¹åŸŽãšããã¢ã³ãâãã«ã®ãã³ã®è£œ
é æ³ã 30 ãã«ãªã¬ãã»ã³çµåã¿ãŒã²ããä»å ã°ãããª
ã³ããã³ãã«ãªã¬ãã»ã³çµåã¢ã³ãâã¬ã³ã°ã
ã³ãããªã矀ã®äžã€ãçµç¹åçã«é©çšããã«åœ
ããã¬ã³ã°ãã³ãã¢ã¹ããã·ãã³ããã³ããªã°
ãã³ãããªã矀ããéžã°ããè¢å ãç¥çµè è «ç
现èã«ãããŠã®ã¿çºçããããšãç¹åŸŽãšããçµ
ç¹åçäžã®ç¥çµè è «ç现èã®ååšã蚺æããæ¹
æ³ã 31 ã¬ã³ã°ãã³ãã¢ã³ãâã¬ã³ã°ãã³ããã³ãã€
ãªã€ãŒãšã¬ã³ã°ãã³ã®è€åäœãããªã矀ããéž
ã°ããäžçš®ããå ç«åŠçæå¹éãåºä¹³åç©ã«æ
äžããããšãç¹åŸŽãšããã¿ãŒã²ããä»å ã°ãã
ãªã³ã®åéãåäžãããæ¹æ³ã 32 ã¬ã³ã°ãã³ 33 ã€ã³ã»ãããã«ãããŠè «ç现èã«å¯Ÿãææ¯ã§
ããã¢ã³ãâã¬ã³ã°ãã³ãåºä¹³åç©ã«æ³šå ¥ãã
ããšã«ãã補é ããããã€ãã«ãªã¬ãã»ã³ãšçµ
åããŠè «ç现èäžã§è¢å ãçããåèš32èšèŒã®
ã¬ã³ã°ãã³ã 34 åèš32èšèŒã®çæç©ã®ã±ã¢ã¬ã·ããã«ãŒã«ã 35 åèš32èšèŒã®çæç©ãšãã€ãªã¢ãŒã®è€åäœã 36 ã±ã¢ã¬ã·ããã«ãŒã«ãšåèš35èšèŒçæç©ã®è€
åäœã 37 人工ç现èå¹å°äžã§ããªã°ãã³åé§äœã補é
ããæ¬¡ãã§ç²ããªã°ãã³å«æãã©ã¯ã·ãšã³ãã
çŽããªã°ãã³ã補é ããããªã°ãã³è£œé æ³ã«ã
ããŠã倧åå¹é€å®¹åšäžã®äººå·¥ç现èå¹å°ã§å¹é€
ããããšãç¹åŸŽãšãããã®æ¹è¯æ³ã 38 æ£åžžãŸãã¯çŸæ£çµç¹ãäžæ§ç·©è¡æ¶²ã§æœåºãè
çœãã©ã¯ã·ãšã³ã坿º¶åããæ¬¡ãã§åŸãããæº¶
è§£æ§èçœæœåºç©ããpKå€çŽïŒãïŒã®ãã©ã¯ã·
ãšã³ãåé¢ããããããååé3000ã25000ã®
çæç©ãåé¢ããããšãç¹åŸŽãšããã¬ã³ã°ãã³
ã®è£œé æ³ã 39 pKå€çŽïŒãïŒã®ãã©ã¯ã·ãšã³åé¢å·¥çšãã
坿º¶æ§èçœè³ªæœåºç©ãã¯ãããã°ã©ãã€ãŒçšã«
ã©ã ã«å ããåŸã ã«é žæ§åºŠãäžããæº¶å€ã§æº¶é¢
ãããããšã«ããéè¡ããåèš38èšèŒã®æ¹æ³ã 40 æº¶é¢æ¶²ã溶åºãããŠã¬ã³ã°ãã³å«æãã©ã¯ã·
ãšã³ãåŸããããèå±€ã²ã«ã¯ãããã°ã©ãã€ãŒ
ã§åé¢ããããšã«ããåé¢ãè¡ãåèš39èšèŒã®
æ¹æ³ã 41 å®éæ²é詊éšããã³å¯å€©å æåæäœã²ã«åæ£
詊éšã«ãããŠãã®ç¹ç°çæäœã§åäžç·ç¶æ²æŸ±ã
圢æãããã€é žæ§ãŸãã¯äžæ§ã®æ°ŽãŸãã¯æ°Žæ§æº¶
æ¶²ã«æº¶è§£ãã¢ã«ã«ãªæ°Žã«ã¯æº¶è§£ããªãåå åžå
æ³¢é·ããŒã¯280ïœÎŒãååéçŽ3000ã25000ã®å
èš38èšèŒã®æ¹æ³ã§è£œé ããã¬ã³ã°ãã³ã
ãããªã°ãã³ãPHïŒãïŒã§å·çœ®ãããïŒã14æ¥åŸ
ã«èå±€ã¯ãããã°ã©ãã€ãŒã§ãã®çæç©ã¯ååé
ãçŽ200æžå°ããããšã瀺ãããã®æº¶æ¶²ãæŽã«é·
ãæŸçœ®ãããšçŽ200ååéã®åäœãïŒã10æ¥ã«é
è£ãããããªãã¡ã¢ã¹ããã·ãã³ã§ã¯8000ããå
åéãæžå°ããããªã°ãã³ã§ã¯10000ããååé
ãæžå°ããåå ŽååŒç¶ããŠçŽ200ã®åäœæžå°ããã ã¢ã¹ããã·ãã³ã®ççååŠçç¹ç°æ§ã¯åçæç©
ã§4000ååéãŸã§ç¶æããããããªã°ãã³ã®çç
ååŠçç¹ç°æ§ã¯åçæç©ã§5000ååéãŸã§ç¶æã
ãããããã¯æâã¢ã¹ããã·ãã³ããã³æâããª
ã°ãã³ã®ããããã«å¯ŸãããªãŠã¯ãã«ãããŒã»ã²
ã«æ¡æ£è©Šéšã§ç€ºãããã ãã®éè£ã¯ãŸãããªãã·ã³ããã³ãã®ä»ã®ãã
ãã¢ãŒãŒãšå ±ã®ããã«é µçŽ çã«ãåãçµæã§éæ
ããããšãã§ããã ã¬ã³ã°ãã³ã®å æ°Žéè£ã«ãã€ãŠèª¿è£œããããã
ãã®ååç©ã®ååéã¯æ¬¡åŒã«ãã€ãŠã»ãŒå®çŸ©ãã
ãã ã¢ã¹ããã·ãã³ã®ççååŠçç¹ç°æ§ãæããç
æç©ã«å¯ŸããŠã¯ïŒ400ïŒ200XïŒïŒ¹ ããªã°ãã³ã®ççååŠçç¹ç°æ§ãæããçæç©
ã«å¯ŸããŠã¯ïŒ5000ïŒ200XïŒïŒ¹ ããã«ïŒ¹ã¯çæç©ã®ååéã§ïŒžã¯ïŒãïŒã®æŽæ°
ã§ããã 宿œäŸ 12 ã¬ã³ã°ãã³ãšã®äººå·¥çµç¹ãŸãã¯åšç®¡ã®è£œé ãã©ã¹ããã¯ãéå±ãŸãã¯ãã®ä»ã®é©åœãªãã
ãææã®ãããå£ã®ç®¡ãã¬ã³ã°ãã³ïŒãã®å Žåã¢
ã¹ããã·ãã³ãŸãã¯ããªã°ãã³ïŒã®é«æ¿åºŠïŒå³ã¡
10mgïŒmlïŒã®ãŽã€ã¹ã³ãŒã¹æº¶æ¶²ãããã¹ãŠã®è¡šé¢
ã被èŠããããŸã§æµžããå«ãŸããããããã¯ãŸã
ã¬ã³ã°ãã³æº¶æ¶²ããã¹ãŠã®è¡šé¢ãååã«è¢«èŠãã
ããŸã§å å§äžã«ç®¡ã®äžããã³åšãéããæ¬¡ã«ç®¡ã
颚也ãŸãã¯æžå§äžã«ä¹Ÿç¥ãããåçµä¹Ÿç¥ããã被
èŠæäœãã¬ã³ã°ãã³ã®å€ãã®ååéã®è¢«èŠãã§ã
ãããã«æ°åç¹°è¿ãã ãã®ç®¡ã¯çããŠããåºä¹³åç©ã®é£æ¥ããçµç¹ãŸ
ãã¯äœæ¶²äžã«ã¢ã¹ããã·ãã³ãŸãã¯ããªã°ãã³ã®
ãããªåé§ç©è³ªã嫿ãã空æŽãŸãã¯çµç¹äžã«çœ®
ãæºåãããããŠã§ããããã®äººå·¥çµç¹ãŸãã¯åš
å®ã¯ã¬ã³ã°ãã³è¢«èŠã®ãªãç°çš®ç©è³ªãåºæ¿ããã
åå¿ãæå°ã«ãããé€ãããã«çšããããã ãã®ä»ã®åœ¢ç¶ã®äººå·¥çµç¹ãŸãã¯åšå®ãåæ§ã«ã
補ããããšãã§ããã ä»¥äžæ¬çºæã®æè¡çç¯å²ã«å å«ãããå ·äœçæ
æ§ãèšèŒããã ïŒ è³è è «è «ççµç¹ãäžæ§ç·©è¡æ¶²ã§æœåºããŠãè
çœè³ªãã©ã¯ã·ãšã³ã坿º¶åãããåŸããã坿º¶
åèçœè³ªåºæ¶²ããpKå€çŽïŒãïŒã®ãã©ã¯ã·ãš
ã³ãåé¢ããããããååéçŽ8000ã®ç©è³ªãå
é¢ããããšãç¹åŸŽãšããã¢ã¹ããã·ãã³ã®è£œé
æ³ã ïŒ pKå€çŽïŒãïŒã®ãã©ã¯ã·ãšã³åé¢å·¥çšãã
坿º¶æ§èçœè³ªæœåºç©ãã¯ãããã°ã©ãã€ãŒçšã«
ã©ã ã«å ããåŸã ã«é žæ§åºŠãäžããæº¶å€ã§æº¶é¢
ãããããšã«ããéè¡ããåèšïŒèšèŒã®æ¹æ³ã ïŒ æº¶é¢æ¶²ã溶åºãããŠã¢ã¹ããã·ãã³å«æãã©
ã¯ã·ãšã³ãåŸããããèå±€ã¯ãããã°ã©ãã€ãŒ
ã§åé¢ããããšã«ããåé¢ãè¡ãåèšïŒèšèŒã®
æ¹æ³ã ïŒ å®éæ²é詊éšããã³å¯å€©å æåæäœã²ã«åæ£
詊éšã«ãããŠãã®ç¹ç°çæäœã§åäžç·ç¶æ²æŸ±ã
圢æãããã€é žæ§ãŸãã¯äžæ§ã®æ°ŽãŸãã¯æ°Žæ§æº¶
æ¶²ã«æº¶è§£ãã¢ã«ã«ãªæ°Žã«ã¯æº¶è§£ããªãåå åžå
æ³¢é·ããŒã¯280ïœÎŒãååéçŽ8000ã®åèšïŒèš
èŒã®æ¹æ³ã§è£œé ããã¢ã¹ããã·ãã³ã ïŒ ã¢ã¹ããã·ãã³ ïŒ å®éæ²æŸ±è©Šéšããã³å¯å€©å æåæäœã²ã«åæ£
詊éšã«ãããŠãã®ç¹ç°çæäœã§æ²æŸ±ã圢æãã
ãã€é žæ§ãŸãã¯äžæ§ã®æ°ŽãŸãã¯æ°Žæ§æº¶æ¶²ã«æº¶è§£
ãã¢ã«ã«ãªã«ã¯æº¶è§£ããªãåå åžåæ³¢é·ããŒã¯
280ïœÎŒãååéçŽ8000ã®åèšïŒèšèŒã®çæç©ã ïŒ ã¢ããé žçµæç©ãå€§ç¥æ¬¡ã®åŠã嫿ãã æ®åºæŠç¥æ° ã¢ã¹ãã©ã®ã³é ž ïŒ ã¹ã¬ãªãã³ ïŒ ãŒãªã³ ïŒ ã°ã«ã¿ãã³é ž 13 ãããªã³ ïŒ ã°ãªã·ã³ ïŒ ã¢ã©ãã³ ïŒ ããªã³ ïŒ 1/2ã·ã¹ãã³ ïŒ ã¡ããªãã³ ïŒ ã€ãœãã€ã·ã³ ïŒ ãã€ã·ã³ ïŒ ããã·ã³ ïŒ ããšãã«ã¢ã©ãã³ ïŒ ãªãžã³ ïŒ ãã¹ããžã³ ïŒ ã¢ã«ã®ãã³ ïŒ åèšïŒæŠç¥ïŒ 88 ãã€ãã¢ããé žã·ã¹ãã³é žãããããã·ãã
ãªã³ããã«ãã€ã·ã³ãã¢ã³ã¢ãã¢ãã€ãœãã¹ã¢
ã·ã³ããã¹ã¢ã·ã³ãããããã·ãªãžã³ããªãžã
ãã«ãã€ã·ã³ããã³Î³âã¢ããé ªé žãæ€åºéã¯
ååšããªãåèšïŒèšèŒã®çæç©ã ïŒ ããã¢ã¢ã»ãã«ã»ã«ããŒã¹ãšè€åäœã圢æ
ããããã¢ã¢ã»ãã«âã»ã«ããŒã¹âã¢ã¹ããã·
ãã³ãšãªãããã€åºä¹³åç©ã«æäžããŠç¹ç°çæ
äœã¢ã³ãâã¢ã¹ãããã³ãçæãã該ã¢ã³ãâ
ã¢ã¹ãããã³ãã€ã³ã»ãããã«ãããŠè³è «çã«
ææ¯ã§ãã€ãŠããã«ãªã¬ãã»ã³ãšçµåãããšè
è «çŽ°èã®è¢å ãçããæ§è³ªãæããåèšïŒèšèŒ
ã®çæç©ã ïŒ ã¢ã³ãâã¢ã¹ãããã³ãããã¢ã¢ã»ãã«ã»ã«
ããŒã¹âã¢ã¹ããã·ãã³ãããã¢ã¢ã»ãã«âã»
ã«ããŒã¹âã¢ã¹ãã°ãã·ãã³âã¢ã³ãâã¢ã¹ã
ãã·ãã³ãã¢ã³ãâããªã°ãã³ãããã¢ã¢ã»ã
ã«ã»ã«ããŒã¹âããªã°ãã³ããã³ããã¢ã¢ã»ã
ã«âã»ã«ããŒã¹âããªã°ãã³âã¢ã³ãâããªã°
ãã³ããªãã矀ããéžã°ããäžçš®ã 10 å¹å°å ã§çè²ãã人工ç现èãäžæ§ç·©è¡æ¶²ã§
æœåºããŠèçœè³ªãã©ã¯ã·ãšã³ã坿º¶åãããåŸ
ãããæœåºæ¶²ããpKå€çŽïŒãïŒã®ãã©ã¯ã·ãš
ã³ãåé¢ããããããååéçŽ10000ã®ç©è³ªã
åé¢ããããšãç¹åŸŽãšããããªã°ãã³ã®è£œé
æ³ã 11 pKå€çŽïŒãïŒã®ãã©ã¯ã·ãšã³ãåé¢ããå·¥
çšãã坿º¶æ§èçœè³ªã®è©²æœåºç©ãã¯ãããã°ã©
ãã€ãŒçšã«ã©ã ã«å ããæŒžæ¬¡é žæ§åºŠããããæº¶
å€ã§æº¶é¢ãããããšã«ããéè¡ããåèš10èšèŒ
ã®æ¹æ³ã 12 溶é¢åºŠã溶åºãããŠããªã°ãã³å«æãã©ã¯ã·
ãšã³ãåŸããããèå±€ã¯ãããã°ã©ãã€ãŒã§å
é¢ããããšã«ããåé¢ãè¡ãåèš11èšèŒã®æ¹
æ³ã 13 å®éæ²é詊éšããã³å¯å€©å æåæäœã²ã«åæ£
詊éšã«ãããŠãã®ç¹ç°çæäœã§åäžç·ç¶æ²æŸ±ã
圢æãããã€é žæ§ãŸãã¯äžæ§ã®æ°ŽãŸãã¯æ°Žæ§æº¶
æ¶²ã«æº¶è§£ãã¢ã«ã«ãªæ°Žã«ã¯æº¶è§£ããªãåå åžå
æ³¢é·ããŒã¯280ïœÎŒãååéçŽ10000ã®åèš10èš
èŒã®æ¹æ³ã§è£œé ããã¢ã¹ããã·ãã³ã 14 ããªã°ãã³ 15 å®éæ²æŸ±è©Šéšããã³å¯å€©å æåæäœã²ã«åæ£
詊éšã«ãããŠãã®ç¹ç°çæäœã§æ²æŸ±ã圢æãã
ãã€é žæ§ãŸãã¯äžæ§ã®æ°ŽãŸãã¯æ°Žæ§æº¶æ¶²ã«æº¶è§£
ãã¢ã«ã«ãªã«ã¯æº¶è§£ããªãåå åžåæ³¢é·ããŒã¯
280ïœÎŒãååéçŽ10000ã®åèš14èšèŒã®çæ
ç©ã 16 ã¢ããé žçµæç©ãå€§ç¥æ¬¡ã®åŠã嫿ãã æ®åºæŠç¥æ° ã¢ã¹ãã©ã®ã³é ž ïŒ ã¹ã¬ãªãã³ ïŒ ãŒãªã³ ïŒ ã°ã«ã¿ãã³é ž 13 ãããªã³ ïŒ ã°ãªã·ã³ ïŒ ã¢ã©ãã³ ïŒ ããªã³ ïŒ 1/2ã·ã¹ãã³ ïŒ ã¡ããªãã³ ïŒ ã€ãœãã€ã·ã³ ïŒ ãã€ã·ã³ ïŒ ããã·ã³ ïŒ ããšãã«ã¢ã©ãã³ ïŒ ãªãžã³ ïŒ ãã¹ããžã³ ïŒ ã¢ã«ã®ãã³ ïŒ åèšïŒæŠç¥ïŒ 89 ãã€ãã¢ããé žã·ã¹ãã³é žãããããã·ãã
ãªã³ããã«ãã€ã·ã³ãã¢ã³ã¢ãã¢ãã€ã¹ãã¹ã¢
ã·ã³ããã¹ã¢ã·ã³ãããããã·ã«ã·ã³ããªãžã
ãã«ãã€ã·ã³ããã³Î³âã¢ããé ªé žãæ€åºéã¯
ååšããªãåèš15èšèŒã®çæç©ã 17 ããã¢ã¢ã»ãã«ã»ã«ããŒã¹ãšè€åäœã圢æ
ããããã¢ã¢ã»ãã«âã»ã«ããŒã¹âããªã°ãã³
ãšãªãããã€åºä¹³åç©ã«æäžããŠç¹ç°çæäœã¢
ã³ãâããªã°ãã³ãçæãã該ã¢ã³ãâããªã°
ãã³ãã€ã³ã»ãããã«ãããŠè³è «çã«ææ¯ã§ã
ã€ãŠããã«ãªã¬ãã»ã³ãšçµåãããšè è «çŽ°èã®
è¢å ãçããæ§è³ªãæããåèšïŒèšèŒã®çæ
ç©ã 18 è¡æž ãŸãã¯ä»ã®äœæ¶²ãããã¢ã¢ã»ãã«ã»ã«ã
ãŒã¹âã¢ã¹ããã·ãã³ãŸãã¯ããã¢ã¢ã»ãã«ã»
ã«ããŒã¹âããªã°ãã³ãšåè§£ã®èµ·ãã¬äœæž©ã§çŽ
ïŒæéãŸãã¯ãã以äžåå¿ãããåŸãããç©è³ª
ãåžé žã§åŠçããŠã¹ããŠâã¿ãŒã²ããä»å ã°ã
ããªã³ãè§£é¢ããããããšãç¹åŸŽãšããã¹ããŠ
âã¿ãŒã²ããä»å ã°ãããªã³ã®è£œé æ³ã 19 ç·©è¡æ°Žæº¶æ¶²ã«æº¶è§£ããå¯å€©å æåæäœã²ã«å
æ£è©Šéšã«ãããŠã¢ã¹ããã·ãã³ãŸãã¯ããªã°ã
ã³ã§åäžç·ç¶æ²æŸ±ã圢æããã»ããã¢ã³èã§é
æãããååé25000以äžã®ååãä¿æããã
ãªãã¢ã»ãã€ã«ã¿ãŒã«ãã€ãŠä¿æãããèå±€ã²
ã«ã¯ãããã°ã©ãã€ãŒã«ããæž¬å®ãããååé
ãåéäœã®ç¶æ ã«ããçŽ50000ããã³ãã¯ãã°
ãããªã³ç¯å²ã«å°ããã®æ°åã®ååéãæãã
åå åžåæ³¢é·ããŒã¯280ïœÎŒãæããåèš18èš
èŒã®æ¹æ³ã§èª¿è£œããã¹ããŠâã¿ãŒã²ããä»å ã°
ãããªã³ã 20 è¡æž ãŸãã¯ä»ã®äœæ¶²ãããã¢ã¢ã»ãã«ã»ã«ã
ãŒã¹âã¢ã¹ããã·ãã³ãŸãã¯ããã¢ã¢ã»ãã«ã»
ã«ããŒã¹âããªã°ãã³ãšåè§£åå¿ã®èµ·ãã¬äœæž©
ã§çŽ10åéåå¿ãããåŸãããç©è³ªãçšé žã§åŠ
çããŠãã¢ã¹ãã¿ãŒã²ããä»å ã°ãããªã³ãåŸ
ãããšãç¹åŸŽãšãããã¢ã¹ãã¿ãŒã²ããä»å ã°
ãããªã³ã®è£œé æ³ã 21 ç·©è¡æ°Žæº¶æ¶²ã«æº¶è§£ããå¯å€©å æåæäœã²ã«å
æ£è©Šéšã«ãããŠã¢ã¹ããã·ãã³ãŸãã¯ããªã°ã
ã³ã§åäžç·ç¶æ²æŸ±ã圢æããã»ããã¢ã³èã§é
æãããååé25000以äžã®ååãä¿æããã
ãªãã¢ã»ãã€ã«ã¿ãŒã«ãã€ãŠä¿æãããèå±€ã²
ã«ã¯ãããã°ã©ãã€ãŒã«ããæž¬å®ãããååé
ãåéäœã®ç¶æ ã«ããçŽ50000ããã³ãã¯ãã°
ãããªã³ç¯å²ã«å°ããã®æ°åã®ååéãæãã
åå åžåæ³¢é·ããŒã¯280ïœÎŒãæããåèš20èš
èŒã®æ¹æ³ã§èª¿è£œããã¹ããŠâã¿ãŒã²ããä»å ã°
ãããªã³ã 22 補é ãããã¹ããŠâã¿ãŒã²ããä»å ã°ãããª
ã³ããã³ãã¢ã¹ãâã¿ãŒã²ããä»å ã°ãããªã³
ã®æ¿åºŠãå ¬ç¥éã®åºä¹³åç©ã®è¡æž ãŸãã¯äœæ¶²ã«
ãã€ãŠæž¬å®ããããšãç¹åŸŽãšããåºä¹³åç©äžã®
çè «çã®æ€åºæ³ã 23 ã¢ã¹ããã·ãã³ã®ç©çååŠçç¹æ§ãæããæ§
é ããæ±ºå®ãããååéãçŽïŒ4000ïŒ200XïŒ
ïŒïŒåŒäžãã¯çæç©ã®ååéãã¯ïŒã19
ã®æŽæ°ïŒãæããçæç©ã 24 ããªã°ãã³ã®ç©çååŠçç¹æ§ãæããæ§é ã
ãæ±ºå®ãããååéãçŽïŒ4000ïŒ200XïŒïŒ¹ïŒ
ïŒåŒäžãã¯çæç©ã®ååéãã¯ïŒã19ã®æŽ
æ°ïŒãæããçæç©ã 25 ã¢ã¹ããã·ãã³ã«å¯Ÿå¿ããæäœãåºä¹³åç©äž
ã«çããããããšãç¹åŸŽãšããæã¢ã¹ããã·ã
ã³ã®è£œé æ³ã 26 ã¢ã¹ããã·ãã³ããã³ããªã°ãã³ãããªã矀
ããéžã°ããã¬ã³ã°ãã³ãšããã¢ã¢ã»ãã«ã»ã«
ããŒã¹ãåºä¹³åç©äžã§æ··åãè€åäœã圢æãã
ãããšãç¹åŸŽãšããããã¢ã¢ã»ãã«ã»ã«ããŒã¹
âã¬ã³ã°ãã³ã®è£œé æ³ã 27 ã¢ã³ãã¬ã³ã°ãã³ãšã¬ã³ã°ãã³ãã¢ã¹ããã·
ãã³ããã³ããªã°ãã³ãããªã矀ããéžã°ãã
ããã¢ã¢ã»ãã«ã»ã«ããŒã¹âã¬ã³ã°ãã³ãåºä¹³
åç©äžã§åå¿ããè€åäœã圢æãããããšãç¹
城ãšããããã¢ã¢ã»ãã«ã»ã«ããŒã¹âã¬ã³ã°ã
ã³âã¢ã³ãâã¬ã³ã°ãã³ã®è£œé æ³ã 28 ã¬ã³ã°ãã³ãã¢ã¹ããã·ãã³ããã³ããªã°ã
ã³ãããªã矀ããéžã°ããããã¢ã¢ã»ãã«ã»ã«
ããŒã¹âã¬ã³ã°ãã³âã¢ã³ãâã¬ã³ã°ãã³ãé ž
ãŸãã¯ãããããŒãŒé µçŽ ã«ããå æ°Žåè§£ããã
ãšãç¹åŸŽãšããã¢ã³ãâã¬ã³ã°ãã³ã®è£œé æ³ã 29 ãã«ã®ãã³ã«å¯Ÿå¿ããæäœãåºä¹³åç©ã«æäž
ããããšãç¹åŸŽãšããã¢ã³ãâãã«ã®ãã³ã®è£œ
é æ³ã 30 ãã«ãªã¬ãã»ã³çµåã¿ãŒã²ããä»å ã°ãããª
ã³ããã³ãã«ãªã¬ãã»ã³çµåã¢ã³ãâã¬ã³ã°ã
ã³ãããªã矀ã®äžã€ãçµç¹åçã«é©çšããã«åœ
ããã¬ã³ã°ãã³ãã¢ã¹ããã·ãã³ããã³ããªã°
ãã³ãããªã矀ããéžã°ããè¢å ãç¥çµè è «ç
现èã«ãããŠã®ã¿çºçããããšãç¹åŸŽãšããçµ
ç¹åçäžã®ç¥çµè è «ç现èã®ååšã蚺æããæ¹
æ³ã 31 ã¬ã³ã°ãã³ãã¢ã³ãâã¬ã³ã°ãã³ããã³ãã€
ãªã€ãŒãšã¬ã³ã°ãã³ã®è€åäœãããªã矀ããéž
ã°ããäžçš®ããå ç«åŠçæå¹éãåºä¹³åç©ã«æ
äžããããšãç¹åŸŽãšããã¿ãŒã²ããä»å ã°ãã
ãªã³ã®åéãåäžãããæ¹æ³ã 32 ã¬ã³ã°ãã³ 33 ã€ã³ã»ãããã«ãããŠè «ç现èã«å¯Ÿãææ¯ã§
ããã¢ã³ãâã¬ã³ã°ãã³ãåºä¹³åç©ã«æ³šå ¥ãã
ããšã«ãã補é ããããã€ãã«ãªã¬ãã»ã³ãšçµ
åããŠè «ç现èäžã§è¢å ãçããåèš32èšèŒã®
ã¬ã³ã°ãã³ã 34 åèš32èšèŒã®çæç©ã®ã±ã¢ã¬ã·ããã«ãŒã«ã 35 åèš32èšèŒã®çæç©ãšãã€ãªã¢ãŒã®è€åäœã 36 ã±ã¢ã¬ã·ããã«ãŒã«ãšåèš35èšèŒçæç©ã®è€
åäœã 37 人工ç现èå¹å°äžã§ããªã°ãã³åé§äœã補é
ããæ¬¡ãã§ç²ããªã°ãã³å«æãã©ã¯ã·ãšã³ãã
çŽããªã°ãã³ã補é ããããªã°ãã³è£œé æ³ã«ã
ããŠã倧åå¹é€å®¹åšäžã®äººå·¥ç现èå¹å°ã§å¹é€
ããããšãç¹åŸŽãšãããã®æ¹è¯æ³ã 38 æ£åžžãŸãã¯çŸæ£çµç¹ãäžæ§ç·©è¡æ¶²ã§æœåºãè
çœãã©ã¯ã·ãšã³ã坿º¶åããæ¬¡ãã§åŸãããæº¶
è§£æ§èçœæœåºç©ããpKå€çŽïŒãïŒã®ãã©ã¯ã·
ãšã³ãåé¢ããããããååé3000ã25000ã®
çæç©ãåé¢ããããšãç¹åŸŽãšããã¬ã³ã°ãã³
ã®è£œé æ³ã 39 pKå€çŽïŒãïŒã®ãã©ã¯ã·ãšã³åé¢å·¥çšãã
坿º¶æ§èçœè³ªæœåºç©ãã¯ãããã°ã©ãã€ãŒçšã«
ã©ã ã«å ããåŸã ã«é žæ§åºŠãäžããæº¶å€ã§æº¶é¢
ãããããšã«ããéè¡ããåèš38èšèŒã®æ¹æ³ã 40 æº¶é¢æ¶²ã溶åºãããŠã¬ã³ã°ãã³å«æãã©ã¯ã·
ãšã³ãåŸããããèå±€ã²ã«ã¯ãããã°ã©ãã€ãŒ
ã§åé¢ããããšã«ããåé¢ãè¡ãåèš39èšèŒã®
æ¹æ³ã 41 å®éæ²é詊éšããã³å¯å€©å æåæäœã²ã«åæ£
詊éšã«ãããŠãã®ç¹ç°çæäœã§åäžç·ç¶æ²æŸ±ã
圢æãããã€é žæ§ãŸãã¯äžæ§ã®æ°ŽãŸãã¯æ°Žæ§æº¶
æ¶²ã«æº¶è§£ãã¢ã«ã«ãªæ°Žã«ã¯æº¶è§£ããªãåå åžå
æ³¢é·ããŒã¯280ïœÎŒãååéçŽ3000ã25000ã®å
èš38èšèŒã®æ¹æ³ã§è£œé ããã¬ã³ã°ãã³ã
第ïŒå³ã¯æ¬çºæã«ãããããªã°ãã³çæéãæª
æ§åºŠåã®é¢æ°ãšããŠç€ºãã°ã©ãã§ããã
æ§åºŠåã®é¢æ°ãšããŠç€ºãã°ã©ãã§ããã
Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ ç¢ºç«ããããŸãã¯åé¢ãããè «ççµç¹çްèã
äžæ§ç·©è¡æ¶²ã§æœåºããŠèçœãã©ã¯ã·ãšã³ã溶解
ããåŸãããèçœæº¶è§£æœåºæ¶²ããpKå€çŽïŒãïŒ
ã®ãã©ã¯ã·ãšã³ãåé¢ããããããååéçŽ3000
ã25000ã®ç©è³ªãåé¢ããããšãç¹åŸŽãšããã¬ã³
ã°ãã³é¡ããªããããã®è£œé æ³ã ïŒ ç¢ºç«ããããŸãã¯åé¢ãããè «ççµç¹çްèã
äžæ§ç·©è¡æ¶²ã§æœåºããŠèçœãã©ã¯ã·ãšã³ã溶解
ããåŸãããèçœæº¶è§£æœåºæ¶²ããpKå€çŽïŒãïŒ
ã®ãã©ã¯ã·ãšã³ãåé¢ããããããååéããã
ããçŽ8000ããã³çŽ10000ã®ç©è³ªã§ããã¢ã¹ãã
ã·ãã³ããã³ããªã°ãã³ããéžã°ããã¬ã³ã°ãã³
ãåé¢ãããããããã¢ã¢ã»ãã«ã»ã«ããŒã¹ãšè€
åãããããšãç¹åŸŽãšããããã¢ã¢ã»ãã«ã»ã«ã
ãŒã¹âã¬ã³ã°ãã³è€åäœã®è£œé æ³ã
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP50125830A JPS5251018A (en) | 1975-10-17 | 1975-10-17 | Productzon of diagnostic agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP50125830A JPS5251018A (en) | 1975-10-17 | 1975-10-17 | Productzon of diagnostic agent |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1518689A Division JPH0216453A (ja) | 1989-01-26 | 1989-01-26 | ã¬ã³ã°ãã³é¡ããã³ãã®çšé |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5251018A JPS5251018A (en) | 1977-04-23 |
| JPH0124800B2 true JPH0124800B2 (ja) | 1989-05-15 |
Family
ID=14919986
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP50125830A Granted JPS5251018A (en) | 1975-10-17 | 1975-10-17 | Productzon of diagnostic agent |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5251018A (ja) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4565789A (en) * | 1983-04-04 | 1986-01-21 | The United States Of America As Represented By The Department Of Health And Human Services | Cell matrix receptor system and use in cancer diagnosis and management |
-
1975
- 1975-10-17 JP JP50125830A patent/JPS5251018A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5251018A (en) | 1977-04-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Seibert | The purification and properties of the purified protein derivative of tuberculin | |
| US4298590A (en) | Detection of malignant tumor cells | |
| US4196186A (en) | Method for diagnosing malignant gliol brain tumors | |
| CA1215919A (en) | Detection of malignant tumor cells | |
| US5866690A (en) | Detection of malignant tumor cells | |
| US4195017A (en) | Malignin, derived from brain tumor cells, complexes and polypeptides thereof | |
| Engelhardt et al. | Localization of alphaâfetoprotein in transplantable murine teratocarcinomas | |
| JPS62181221A (ja) | å ç«äžå šçå矀治ççšå»è¬çµæç© | |
| Sheffield et al. | Studies on plasma membranes: XVI. Tissue specific antigens in the liver cell surface | |
| US4624932A (en) | Recognins and their chemoreciprocals | |
| KR850001770B1 (ko) | ìžì²Ž ì íìì ëí í¹ìŽí첎ì ì ì¡°ë°©ë² | |
| Tsuchida et al. | Alpha-fetoprotein, prealbumin, albumin, alpha-l-antitrypsin and transferrin as diagnostic and therapeutic markers for endodermal sinus tumors | |
| JPH0146520B2 (ja) | ||
| EP0007214A1 (en) | Product for detecting the presence of cancerous or malignant tumor cells | |
| JPH0124800B2 (ja) | ||
| JPH0260983B2 (ja) | ||
| US4840915A (en) | Method for diagnosing malignant tumors | |
| JPH0321560B2 (ja) | ||
| Walker et al. | Chapter VII The Localization of Bacterial Antigens by the Use of the Fluorescent and Ferritin Labelled Antibody Techniques | |
| US4624931A (en) | Recognins and their chemoreciprocals | |
| Saurat et al. | Pr antigens in the skin: distinct localization linked to the stage and the type of keratinocyte differentiation | |
| EP0015755B1 (en) | Recognins, their chemoreciprocals, target attaching globulins and processes for producing these products; methods of detecting cancer tumors | |
| US4976957A (en) | Process for the production of recognins and their chemoreciprocals | |
| JPH04355339A (ja) | åŸ®éæ€äœæ¡åçšå · | |
| CA1057684A (en) | Recognins and their chemoreciprocals |