JPH03280836A - Feed additive for ruminant - Google Patents

Abstract

PURPOSE:To obtain the title additive protecting a biologically active substance from gastric juice in rumen and efficiently absorbing said substance in digestive organs in and after abomasum by coating core granules containing a biologically active substance and a protective substance with a specific protecting coating film. CONSTITUTION:Core granules containing both a biologically active substance (preferably methionine, lysine hydrochloride or vitamins) and a protective substance (preferably the same kind of fatty acid salt as that of protecting coating film) are coated with a protecting coating film (preferably calcium salt of a mixed fatty acids having 30-50 deg.C melting point) of fatty acid salt which is insoluble in a neutral range and shows decomposing properties in an acidic range to give the objective additive.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a feed additive for ruminants, and more particularly, it protects biologically active substances from the gastric juice of the rumen of ruminants. The present invention relates to a preparation in which core granules containing a biologically active substance are coated with a protective coating of a fatty acid salt in order to be efficiently absorbed in the digestive organs starting from the four stomachs.

The feed additive for ruminants of the present invention can be added to concentrated feed and orally administered to ruminants such as cattle and sheep, and can efficiently absorb biologically active substances such as amino acids, proteins, and veterinary drugs. It is suitably used as a formulation for

[Conventional technology]

When biologically active substances such as amino acids, proteins, and veterinary drugs are orally administered to ruminants, they are degraded by microorganisms present in the gastric juice of the rumen of the ruminant, so the biologically active substances are not effectively absorbed. .

Therefore, in order to efficiently absorb these biologically active substances, the biologically active substances are coated and protected with a substance that is stable against the ruminal fluid, such as oil, and the digestive organs from the abomasum onward are protected. Various feed additives for ruminants that release biologically active substances have been proposed (Japanese Unexamined Patent Publication No. 56-15).
4956, Japanese Unexamined Patent Publication No. 61-151133, etc.), the applicant of the present invention also added a biologically active substance to a protective substance such as hydrogenated oil so that the protective substance disintegrates in the abomasum and subsequent digestive organs. A preparation protected by adding chitosan for the purpose of imparting has been proposed and published under the name Lafchit (see JP-A-58-175449, JP-A-59-198946, etc.).

On the other hand, as an energy source that bypasses the rumen of ruminants and is absorbed with high efficiency in the digestive organs after the abomasum, calcium of fatty acids having 14.16 and/or 18 carbon atoms,
Divalent metal salts such as magnesium have been proposed (LISP 4
, 826, 694), and calcium salts of mixed fatty acids produced from natural fats and oils (hereinafter referred to as "bypass fats and oils") are commercially available.

There is also a known method for producing feed additives for ruminants by heating these bypass fats and oils to a temperature above their melting point to soften them, adding and mixing biologically active substances therein, and then cooling and solidifying the mixture. (Refer to Japanese Patent Application Laid-Open No. 63-313546).

[Problem that the invention seeks to solve]

In feed additives for ruminants in which biologically active substances are coated and protected with the above-mentioned protective substances such as hydrogenated oil, the biologically active substances have ruminal bypass properties and are released in the digestive tract from the abomasum onward. However, there were problems with storage stability, especially thermal stability at high temperatures of 40°C or higher.

On the other hand, bypass oils have higher melting points and better thermal stability than hydrogenated oils, etc., so preparations in which biologically active substances are coated and protected with bypass oils are expected to have excellent thermal stability. . However, in the preparations manufactured by the method cited above, when a biologically active substance is contained in a high concentration or a highly water-soluble biologically active substance is contained, various factors come together and the biological The active substance exhibits little ruminal bypassing properties.

An object of the present invention is to provide an anti-impulse feed additive that has excellent ruminal bypass properties for biologically active substances.

[Means for solving problems]

As a result of intensive research to achieve the above object, the present inventors have developed nuclear granules containing biologically active substances and protective substances.
Furthermore, it was discovered that the rumen bypass property was significantly improved by coating with a protective film made of a divalent metal salt of a fatty acid, and the present invention was completed based on this finding.

The present invention provides a method for use in ruminants, characterized in that core granules containing a biologically active substance and a protective substance are coated with a protective coating of a fatty acid salt that is insoluble in a neutral range and decomposable in an acidic range. It is a feed additive.

In the present invention, biologically active substances are substances that are given to animals and exhibit activities such as promoting fattening, preventing diseases, and treating diseases. In particular, when orally administered to ruminants, microorganisms present in the rumen It is a substance that is difficult to utilize effectively as it is.

For example, amino acids: methionine, lysine, tryptophan, etc., N-acyl amino acids, N-stearoylmethionine, N-oleylmethionine.

Amino acid salts, such as the calcium salt of N-hydroxymethylmethionine: lysine hydrochloride, etc., hydroxy analogues of amino acids: 2-hydroxy-4-methylmercaptobutyric acid and its calcium salt, etc., Proteins: powder-free,
Casein, potato protein, soybean protein, etc., vitamins: vitamin A, vitamin A acetate, vitamin A palmitate, vitamin D! + Vitamin E, nicotinic acid and nicotinamide, calcium pantothenate.

β-carotene, etc., enzymes: acid protease, etc., carbohydrates: glucose, etc., veterinary drugs: penicillin.

Examples include antibiotics such as tetracycline, anthelmintics such as Negfon, and one or more of these may be used.

The protective substance used as a binder during the production of nuclear granules is
Any material that is stable in the rumen juice of ruminants in the neutral pH range of 5 to 8 and decomposed in the abomasal gastric juice in the strongly acidic range of pH 3 or less or the digestive juices of the digestive organs after the abomasum may be used. .

For example, linear or branched saturated or unsaturated monocarboxylic acids having 8 to 22 carbon atoms and their salts, hardened animal and vegetable oils, etc. may be mentioned, and one or more of these may be used. Preferably, divalent metal salts of linear or branched saturated or unsaturated fatty acids having 14 to 18 carbon atoms are used, and more preferably their calcium salts are used.

Core granules are made by granulating one or more of the biologically active substances mentioned above and using a protective substance as a binder, and are usually made by adding biologically active substance powder to the protective substance that has been softened by heat to make it homogeneous. It is manufactured by mixing the mixture with the following ingredients, cooling it, solidifying it, and granulating it.

As the granulation method, a conventional granulation method such as an extrusion granulation method or an agitation granulation method can be employed.

It is preferable that the nuclear granules have a shape closer to a spherical shape.

The fatty acid salt used for the protective film is insoluble in a neutral range and degradable in an acidic range, and is a salt of a fatty acid that is stable in the ruminal juice of a ruminant and decomposed in the abomasal juice, similar to the above-mentioned protective substance. , for example, the carbon number 14.16 and /
Alternatively, calcium salts or magnesium salts of 18 fatty acids, calcium salts of mixed fatty acids produced from natural oils and fats, etc. are used.

Preferably the melting point is 30 to 50°C, more preferably 35°C.
Use calcium salts of mixed fatty acids at ~45°C.

The core granules can be easily coated with a protective film by heating the core granules to the extent that their surfaces are slightly melted using a Henschel mixer, etc., and stirring the core granules while adding the powder of the coating substance. be able to.

[For production]

The present invention is characterized in that, as described above, the core granules containing biologically active substances are further coated with a protective film made of a fatty acid salt that is insoluble in a neutral range and degradable in an acidic range. It is a feed additive for ruminants.

In the present invention, since the protective film is insoluble in the neutral range, it is extremely stable in the rumen gastric fluid of ruminants in the pH range of 5 to 8, and is degradable in the acidic range, so it has a pH of 3 or lower. Easily degraded in the abomasum of ruminants. As a result, the biologically active substances contained in the nuclear granules are eluted in the abomasum of the ruminant and are efficiently absorbed in the subsequent digestive organs. Furthermore, the fatty acid salt used in the protective film is also decomposed and absorbed in the digestive organs starting from the abomasum, and is used as an energy source for ruminants.

In addition, by using a protective substance that is insoluble in the neutral range and decomposable in the acidic range as a binder during the production of nuclear granules, it is possible to improve the ruminal bypass properties of biologically active substances and to improve the ability of the biologically active substances to be absorbed in the digestive organs from the abomasum onwards. Improves release properties. In particular, by using the same type of fatty acid calcium as the protective coating, the binding force between the core granules and the coating is strengthened, and as a result, the destruction of the preparation due to rumination is reduced, further increasing the ruminal bypass rate of biologically active substances. improves.

In addition, this formulation has a high softening temperature, so it not only has excellent thermal stability during storage, but also can be added as an ingredient when pelletizing concentrate feed to administer biologically active substances to anti-impulse foods. It is also effective as

〔Example〕

The present invention will be explained in more detail by Examples and Comparative Examples.

However, the scope of the present invention is not limited in any way by the following examples.

In addition, in the following steel, "1 part" and "%" are based on weight unless otherwise specified.

(1) Preparation of core granules A calcium salt of beef tallow fatty acid with a melting point of 43°C and methionine powder or risin hydrochloride powder are mixed, extruded into a string using an extruder, and hot cut to have a particle size of 0.5. ~3
Nuclear granules of mm: A-1 to A-6 were prepared.

The composition and particle size of the obtained core granules are shown in Table 1.

(2) Preparation of feed additive for ruminants 100μ of fatty acid calcium used in the above item (1)
m or less powder and core granules prepared in the above item (1):
A-1-A-6 was charged into a stirring device, stirred while heating,
A feed additive for ruminants was prepared in which the surface of nuclear granules was coated with a protective film of fatty acid calcium.

The coating amount and biologically active substance content of the obtained feed additive for ruminants are shown in Table 1.

(3) Dissolution test of biologically active substances 2 g of each of the ruminant feed additive prepared in the above item (2) and the nuclear granules prepared in the above item (1) for comparison were administered to cattle. It was immersed in 200 cc of Tris buffer corresponding to the ruminal juice of a cow, and kept shaking at a temperature of 37°C for 24 hours, and then taken out from the Tris buffer and 0, 05 MC=mo Il corresponding to the rumen juice of a cow.・dm-
3) It was immersed in 200 cc of hydrochloric acid and further shaken at a temperature of 37° C. for 4 hours. Next, the preparation taken out from the 0.05 M hydrochloric acid was immersed in 200 cc of bovine small intestine solution and heated to 37°C.
The mixture was further shaken for 4 hours at a temperature of .

Tris buffer Tris [) Lis (hydroxymethyl) aminomethane]
6.06 g was dissolved in 292 ml of 0.1 M hydrochloric acid, diluted to 1000 ml with water, pH 8.0, and the methionine and lysine eluted in Tris buffer, 0.05 M hydrochloric acid, and small intestine corresponding solution were added. , determined by iodometry or ninhydrin colorimetry.

The test results are shown in Table 1.

As shown in Table 1, in the feed additive for ruminants coated with the protective film of fatty acid calcium of the present invention, the dissolution rate of biologically active substances in the rumen fluid is extremely low, and the Most of the remainder is eluted in the gastric and small intestine fluids.

On the other hand, in preparations that are not coated with a protective film (see Comparative Example), the dissolution rate of biologically active substances in the ruminal fluid is large, and only about 50% of the biologically active substances are dissolved in the ruminal fluid. Do not bypass.

〔Effect of the invention〕

As shown in the examples above, the feed additive for ruminants of the present invention has extremely excellent rumen bypass properties for the biologically active substances contained therein when orally administered to ruminants.

Furthermore, the fatty acid salt used to impart bypass properties is also decomposed and absorbed in the digestive organs starting from the abomasum, and is effectively used as an energy source for ruminants.

The present invention is directed to a ruminant animal suitable for bypassing the rumen and absorbing biologically active substances that are easily degraded in the rumen of the ruminant when administered orally and with high efficiency in the digestive organs from the abomasum onwards. It provides feed additives for livestock, and its significance in industry, especially in the livestock field, is extremely large.

Claims (3)

[Claims] (1) Feed for ruminants, characterized in that core granules containing biologically active substances and protective substances are coated with a protective coating of fatty acid salts that is insoluble in neutral ranges and decomposable in acidic ranges. Additive (2) A feed additive for ruminants according to claim (1), wherein the biologically active substance is at least one selected from the group consisting of methionine, lysine hydrochloride, and vitamins. (3) A feed additive for ruminants according to claim (1), wherein the protective substance is a fatty acid salt of the same type as the protective film (4)
In claim (1), the protective film has a melting point of 30 to 50.
Feed additive for ruminants that is a calcium salt of mixed fatty acids in the range of °C