JPH0573392B2 - - Google Patents
Info
- Publication number
- JPH0573392B2 JPH0573392B2 JP61122723A JP12272386A JPH0573392B2 JP H0573392 B2 JPH0573392 B2 JP H0573392B2 JP 61122723 A JP61122723 A JP 61122723A JP 12272386 A JP12272386 A JP 12272386A JP H0573392 B2 JPH0573392 B2 JP H0573392B2
- Authority
- JP
- Japan
- Prior art keywords
- trna synthetase
- enzyme
- immobilized
- kinase
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000004190 Enzymes Human genes 0.000 claims description 26
- 108090000790 Enzymes Proteins 0.000 claims description 26
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims description 22
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 claims description 22
- 229960001456 adenosine triphosphate Drugs 0.000 claims description 22
- 238000006243 chemical reaction Methods 0.000 claims description 20
- YOAHKNVSNCMZGQ-XPWFQUROSA-N P(1),P(4)-bis(5'-adenosyl) tetraphosphate Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1COP(O)(=O)OP(O)(=O)OP(O)(=O)OP(O)(=O)OC[C@H]([C@@H](O)[C@H]1O)O[C@H]1N1C(N=CN=C2N)=C2N=C1 YOAHKNVSNCMZGQ-XPWFQUROSA-N 0.000 claims description 14
- 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 claims description 11
- 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 claims description 11
- 150000001413 amino acids Chemical class 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 6
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- 239000001177 diphosphate Substances 0.000 claims description 2
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims 1
- 235000011180 diphosphates Nutrition 0.000 claims 1
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- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 19
- 239000007795 chemical reaction product Substances 0.000 description 17
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- LIPOUNRJVLNBCD-UHFFFAOYSA-N acetyl dihydrogen phosphate Chemical compound CC(=O)OP(O)(O)=O LIPOUNRJVLNBCD-UHFFFAOYSA-N 0.000 description 14
- 102000017737 Lysine-tRNA Ligase Human genes 0.000 description 11
- 108010092041 Lysine-tRNA Ligase Proteins 0.000 description 11
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 11
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- 241000193385 Geobacillus stearothermophilus Species 0.000 description 9
- 108010071170 Leucine-tRNA ligase Proteins 0.000 description 9
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- 238000000034 method Methods 0.000 description 9
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 8
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- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 7
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- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 5
- 108010093096 Immobilized Enzymes Proteins 0.000 description 5
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 5
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- 150000001875 compounds Chemical class 0.000 description 4
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- 241000193830 Bacillus <bacterium> Species 0.000 description 3
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- QCICUPZZLIQAPA-XPWFQUROSA-N P(1),P(3)-bis(5'-adenosyl) triphosphate Chemical group C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1COP(O)(=O)OP(O)(=O)OP(O)(=O)OC[C@H]([C@@H](O)[C@H]1O)O[C@H]1N1C(N=CN=C2N)=C2N=C1 QCICUPZZLIQAPA-XPWFQUROSA-N 0.000 description 3
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- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 2
- OLXZPDWKRNYJJZ-UHFFFAOYSA-N 5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-ol Chemical compound C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(CO)O1 OLXZPDWKRNYJJZ-UHFFFAOYSA-N 0.000 description 2
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- 101710096715 Probable histidine-tRNA ligase, cytoplasmic Proteins 0.000 description 2
- 241000589596 Thermus Species 0.000 description 2
- YDHWWBZFRZWVHO-UHFFFAOYSA-H [oxido-[oxido(phosphonatooxy)phosphoryl]oxyphosphoryl] phosphate Chemical compound [O-]P([O-])(=O)OP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O YDHWWBZFRZWVHO-UHFFFAOYSA-H 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
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- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 2
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- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 235000004554 glutamine Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 229940050906 magnesium chloride hexahydrate Drugs 0.000 description 1
- DHRRIBDTHFBPNG-UHFFFAOYSA-L magnesium dichloride hexahydrate Chemical compound O.O.O.O.O.O.[Mg+2].[Cl-].[Cl-] DHRRIBDTHFBPNG-UHFFFAOYSA-L 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 229910001437 manganese ion Inorganic materials 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000027405 negative regulation of phosphorylation Effects 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 229920001308 poly(aminoacid) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 108010064470 polyaspartate Proteins 0.000 description 1
- 108020000161 polyphosphate kinase Proteins 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 235000008521 threonine Nutrition 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/36—Dinucleotides, e.g. nicotineamide-adenine dinucleotide phosphate
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/832—Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Description
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Claims (1)
- ïŒ ã¢ããã¢ã·ã«âtRNAåæé µçŽ ã®è§Šåªäœçšã«
ãããã¢ããã·ã³â5â²âäžãªã³é žåã¯ãã®èªå°äœ
ãšã¢ããé žãšãåå¿ãããŠãžã¢ããã·ã³åãªã³é ž
åã¯ãã®èªå°äœã補é ããã«éãã該åå¿ãã¢ã
ãã·ã³â5â²âäºãªã³é žãã¢ããã·ã³â5â²âäžãªã³
é žã«å€æããé µçŽ ã®ååšäžã§è¡ãããšãç¹åŸŽãšã
ããžã¢ããã·ã³åãªã³é žåã¯ãã®èªå°äœã®è£œé æ¹
æ³ã
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61122723A JPS62278992A (ja) | 1986-05-28 | 1986-05-28 | ãžã¢ããã·ã³åãªã³é žåã¯ãã®èªå°äœã®è£œé æ¹æ³ |
| EP87304628A EP0247819B1 (en) | 1986-05-28 | 1987-05-26 | Process for producing diadenosine tetraphosphate and derivatives thereof |
| DE8787304628T DE3771577D1 (de) | 1986-05-28 | 1987-05-26 | Verfahren zur herstellung von diadenosintetraphosphat und derivaten davon. |
| US07/054,975 US4886749A (en) | 1986-05-28 | 1987-05-28 | Process for producing diadenosine tetraphosphate and derivatives thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61122723A JPS62278992A (ja) | 1986-05-28 | 1986-05-28 | ãžã¢ããã·ã³åãªã³é žåã¯ãã®èªå°äœã®è£œé æ¹æ³ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62278992A JPS62278992A (ja) | 1987-12-03 |
| JPH0573392B2 true JPH0573392B2 (ja) | 1993-10-14 |
Family
ID=14842994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61122723A Granted JPS62278992A (ja) | 1986-05-28 | 1986-05-28 | ãžã¢ããã·ã³åãªã³é žåã¯ãã®èªå°äœã®è£œé æ¹æ³ |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US4886749A (ja) |
| EP (1) | EP0247819B1 (ja) |
| JP (1) | JPS62278992A (ja) |
| DE (1) | DE3771577D1 (ja) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5049550A (en) * | 1987-11-05 | 1991-09-17 | Worcester Foundation For Experimental Biology | Diadenosine 5', 5'"-p1, p4,-tetraphosphate analogs as antithrombotic agents |
| WO1989004321A1 (en) * | 1987-11-05 | 1989-05-18 | The Worcester Foundation For Experimental Biology | Diadenosine 5', 5'''-p1, p4-tetraphosphate and analogs thereof as antithrombotic agents |
| JP2783880B2 (ja) * | 1989-11-24 | 1998-08-06 | å¯å£«ã¬ããªæ ªåŒäŒç€Ÿ | å¿èç æ²»çå€ |
| JPH05286861A (ja) * | 1992-04-06 | 1993-11-02 | Fujirebio Inc | äœè¡å§ç¶æå€ |
| US5569650A (en) * | 1993-06-11 | 1996-10-29 | Sloan-Kettering Institute For Cancer Research | C-nucleoside isosters of analogs thereof and pharmaceutical compositions |
| GB9320563D0 (en) * | 1993-10-06 | 1993-11-24 | Zeneca Ltd | Assay procedure and application in identification of herbicides |
| JPH0812581A (ja) | 1994-06-30 | 1996-01-16 | Fujirebio Inc | èè¡æ§å¿çŸæ£æ²»çå€ |
| US5688670A (en) * | 1994-09-01 | 1997-11-18 | The General Hospital Corporation | Self-modifying RNA molecules and methods of making |
| JP3487939B2 (ja) * | 1995-01-11 | 2004-01-19 | ãŠããã«æ ªåŒäŒç€Ÿ | ãžã¢ããã·ã³ïŒãªã³é žïŒãããªãŠã å¡©ïŒïŒæ°Žåç©çµæ¶ |
| KR100203919B1 (ko) * | 1996-10-04 | 1999-06-15 | ì ëê¶ | ìì©ì± ëšë°±ì§ì ìì°íë ìë¡ìŽ ë°í íëŒì€ë¯žë |
| GB201115793D0 (en) * | 2011-09-13 | 2011-10-26 | Univ Warwick | Screening method |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5225088A (en) * | 1975-08-22 | 1977-02-24 | Kazutomo Imahori | Process for preparing a novel thermoresistant acetate kinase |
| JPS5311589A (en) * | 1976-07-20 | 1978-02-02 | Toshiba Corp | Diffraction grating coupling type semiconductor laser |
| JPS6016231B2 (ja) * | 1979-11-22 | 1985-04-24 | ãŠããã«æ ªåŒäŒç€Ÿ | èç±æ§é ¢é žããâãŒã®è£œé æ³ |
| JPS6016232B2 (ja) * | 1980-01-14 | 1985-04-24 | åå ä»å | ã¢ããã·ã³ããªãªã³é žåçç£çšé µçŽ ã®è£œé æ³ |
| US4331762A (en) * | 1980-04-18 | 1982-05-25 | Unitika Ltd. | Bacillus stearothermophilus strain UK 788 and process for producing a useful enzyme |
| JPS5933355B2 (ja) * | 1980-04-30 | 1984-08-15 | çååŠç 究æ | åºå®åèç±æ§é ¢é žããâ㌠|
| CA1173768A (en) * | 1980-10-09 | 1984-09-04 | Kazuhiko Nagata | Adenylate kinase and process for the production thereof |
| EP0084975B1 (en) * | 1982-01-26 | 1986-12-30 | Imahori Kazutomo | Process for producing physiologically active substance by multienzyme process and apparatus for the same |
| DE3361649D1 (en) * | 1982-01-26 | 1986-02-13 | Imahori Kazutomo | Process for synthesizing peptides or peptide derivatives |
| JPH0797986B2 (ja) * | 1986-08-18 | 1995-10-25 | ãã€ã»ã«ååŠå·¥æ¥æ ªåŒäŒç€Ÿ | èç±æ§é ¢é žããâãŒåã³ãã®è£œé æ³ |
-
1986
- 1986-05-28 JP JP61122723A patent/JPS62278992A/ja active Granted
-
1987
- 1987-05-26 DE DE8787304628T patent/DE3771577D1/de not_active Expired - Fee Related
- 1987-05-26 EP EP87304628A patent/EP0247819B1/en not_active Expired - Lifetime
- 1987-05-28 US US07/054,975 patent/US4886749A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US4886749A (en) | 1989-12-12 |
| DE3771577D1 (de) | 1991-08-29 |
| EP0247819B1 (en) | 1991-07-24 |
| EP0247819A3 (en) | 1989-02-15 |
| JPS62278992A (ja) | 1987-12-03 |
| EP0247819A2 (en) | 1987-12-02 |
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