JPS61132183A - サイクロデキストリン　グルカノトランスフエラ−ゼの製造法 - Google Patents

サイクロデキストリン　グルカノトランスフエラ−ゼの製造法

Info

Publication number: JPS61132183A
Authority: JP; Japan
Prior art keywords: cyclodextrin glucanotransferase; bacillus subtilis; cyclodextrin; culture; restriction enzyme
Prior art date: 1984-12-03
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

JP25421684A

Other languages

English (en)

Japanese (ja)

Other versions

JPH0355107B2 (de

Inventor

Toshiya Takano

高野　敏弥

Shoichi Kobayashi

昭一小林

Hideo Tabei

英夫田部井

Keiji Kainuma

圭二貝沼

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

NORIN SUISANSYO SHOKUHIN SOGO KENKYUSHO

Original Assignee

NORIN SUISANSYO SHOKUHIN SOGO KENKYUSHO

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-12-03

Filing date

1984-12-03

Publication date

1986-06-19

1984-12-03 Application filed by NORIN SUISANSYO SHOKUHIN SOGO KENKYUSHO filed Critical NORIN SUISANSYO SHOKUHIN SOGO KENKYUSHO

1984-12-03 Priority to JP25421684A priority Critical patent/JPS61132183A/ja

1986-06-19 Publication of JPS61132183A publication Critical patent/JPS61132183A/ja

1991-08-22 Publication of JPH0355107B2 publication Critical patent/JPH0355107B2/ja

Status Granted legal-status Critical Current

Links

238000004519 manufacturing process Methods 0.000 title claims description 5
229920000858 Cyclodextrin Polymers 0.000 title description 4
HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 title description 4
108010025880 Cyclomaltodextrin glucanotransferase Proteins 0.000 claims abstract description 35
244000063299 Bacillus subtilis Species 0.000 claims abstract description 16
235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 16
238000012258 culturing Methods 0.000 claims abstract description 6
108091008146 restriction endonucleases Proteins 0.000 abstract description 15
239000012634 fragment Substances 0.000 abstract description 11
210000000349 chromosome Anatomy 0.000 abstract description 8
241000178960 Paenibacillus macerans Species 0.000 abstract description 6
102000012410 DNA Ligases Human genes 0.000 abstract description 2
108010061982 DNA Ligases Proteins 0.000 abstract description 2
238000000354 decomposition reaction Methods 0.000 abstract description 2
238000011109 contamination Methods 0.000 abstract 1
239000012535 impurity Substances 0.000 abstract 1
239000000126 substance Substances 0.000 abstract 1
239000000243 solution Substances 0.000 description 16
239000013612 plasmid Substances 0.000 description 13
239000002609 medium Substances 0.000 description 10
230000001580 bacterial effect Effects 0.000 description 9
210000004027 cell Anatomy 0.000 description 9
239000013611 chromosomal DNA Substances 0.000 description 9
229930027917 kanamycin Natural products 0.000 description 9
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 9
229960000318 kanamycin Drugs 0.000 description 9
229930182823 kanamycin A Natural products 0.000 description 9
229920002472 Starch Polymers 0.000 description 8
238000000034 method Methods 0.000 description 8
239000008107 starch Substances 0.000 description 8
235000019698 starch Nutrition 0.000 description 8
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 7
239000000203 mixture Substances 0.000 description 7
239000002245 particle Substances 0.000 description 7
229940041514 candida albicans extract Drugs 0.000 description 6
238000003776 cleavage reaction Methods 0.000 description 6
230000000694 effects Effects 0.000 description 6
239000000047 product Substances 0.000 description 6
230000007017 scission Effects 0.000 description 6
239000012138 yeast extract Substances 0.000 description 6
241000894006 Bacteria Species 0.000 description 5
241000588724 Escherichia coli Species 0.000 description 5
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
108090000790 Enzymes Proteins 0.000 description 4
102000004190 Enzymes Human genes 0.000 description 4
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
229940088598 enzyme Drugs 0.000 description 4
241000193830 Bacillus <bacterium> Species 0.000 description 3
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
239000000872 buffer Substances 0.000 description 3
239000001110 calcium chloride Substances 0.000 description 3
229910001628 calcium chloride Inorganic materials 0.000 description 3
230000002759 chromosomal effect Effects 0.000 description 3
238000002298 density-gradient ultracentrifugation Methods 0.000 description 3
239000000706 filtrate Substances 0.000 description 3
239000008103 glucose Substances 0.000 description 3
239000001963 growth medium Substances 0.000 description 3
238000000338 in vitro Methods 0.000 description 3
229910052757 nitrogen Inorganic materials 0.000 description 3
108090000623 proteins and genes Proteins 0.000 description 3
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
229920001817 Agar Polymers 0.000 description 2
229920002261 Corn starch Polymers 0.000 description 2
241000701959 Escherichia virus Lambda Species 0.000 description 2
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
229930006000 Sucrose Natural products 0.000 description 2
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
238000005273 aeration Methods 0.000 description 2
239000008272 agar Substances 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
239000008120 corn starch Substances 0.000 description 2
229940099112 cornstarch Drugs 0.000 description 2
239000013078 crystal Substances 0.000 description 2
239000011521 glass Substances 0.000 description 2
229910052740 iodine Inorganic materials 0.000 description 2
239000011630 iodine Substances 0.000 description 2
235000013379 molasses Nutrition 0.000 description 2
238000004816 paper chromatography Methods 0.000 description 2
BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
238000011218 seed culture Methods 0.000 description 2
239000005720 sucrose Substances 0.000 description 2
239000000725 suspension Substances 0.000 description 2
NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
102000008186 Collagen Human genes 0.000 description 1
108010035532 Collagen Proteins 0.000 description 1
GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
241000588748 Klebsiella Species 0.000 description 1
239000001888 Peptone Substances 0.000 description 1
108010080698 Peptones Proteins 0.000 description 1
239000002202 Polyethylene glycol Substances 0.000 description 1
238000002105 Southern blotting Methods 0.000 description 1
241001441724 Tetraodontidae Species 0.000 description 1
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
238000009825 accumulation Methods 0.000 description 1
239000008351 acetate buffer Substances 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
238000001042 affinity chromatography Methods 0.000 description 1
102000004139 alpha-Amylases Human genes 0.000 description 1
108090000637 alpha-Amylases Proteins 0.000 description 1
229940024171 alpha-amylase Drugs 0.000 description 1
150000003863 ammonium salts Chemical class 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
229960002685 biotin Drugs 0.000 description 1
235000020958 biotin Nutrition 0.000 description 1
239000011616 biotin Substances 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
229910052799 carbon Inorganic materials 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
238000010367 cloning Methods 0.000 description 1
229920001436 collagen Polymers 0.000 description 1
238000004440 column chromatography Methods 0.000 description 1
239000000356 contaminant Substances 0.000 description 1
210000004748 cultured cell Anatomy 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
238000010586 diagram Methods 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
238000010828 elution Methods 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
239000000284 extract Substances 0.000 description 1
238000000605 extraction Methods 0.000 description 1
235000013312 flour Nutrition 0.000 description 1
235000001727 glucose Nutrition 0.000 description 1
235000011187 glycerol Nutrition 0.000 description 1
238000004128 high performance liquid chromatography Methods 0.000 description 1
238000009776 industrial production Methods 0.000 description 1
239000003456 ion exchange resin Substances 0.000 description 1
229920003303 ion-exchange polymer Polymers 0.000 description 1
239000007788 liquid Substances 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
235000013372 meat Nutrition 0.000 description 1
229910052751 metal Inorganic materials 0.000 description 1
239000002184 metal Substances 0.000 description 1
150000002739 metals Chemical class 0.000 description 1
229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
235000019796 monopotassium phosphate Nutrition 0.000 description 1
150000002823 nitrates Chemical class 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
235000019319 peptone Nutrition 0.000 description 1
PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
229920001223 polyethylene glycol Polymers 0.000 description 1
229910052700 potassium Inorganic materials 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
210000001938 protoplast Anatomy 0.000 description 1
238000000746 purification Methods 0.000 description 1
238000005185 salting out Methods 0.000 description 1
230000003248 secreting effect Effects 0.000 description 1
239000006152 selective media Substances 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
239000002904 solvent Substances 0.000 description 1
238000005507 spraying Methods 0.000 description 1
230000002463 transducing effect Effects 0.000 description 1
230000026683 transduction Effects 0.000 description 1
238000010361 transduction Methods 0.000 description 1
238000011426 transformation method Methods 0.000 description 1
238000005406 washing Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Enzymes And Modification Thereof (AREA)

JP25421684A 1984-12-03 1984-12-03 サイクロデキストリン　グルカノトランスフエラ−ゼの製造法 Granted JPS61132183A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP25421684A JPS61132183A (ja)	1984-12-03	1984-12-03	サイクロデキストリン　グルカノトランスフエラ−ゼの製造法

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP25421684A JPS61132183A (ja)	1984-12-03	1984-12-03	サイクロデキストリン　グルカノトランスフエラ−ゼの製造法

Publications (2)

Publication Number	Publication Date
JPS61132183A true JPS61132183A (ja)	1986-06-19
JPH0355107B2 JPH0355107B2 (de)	1991-08-22

Family

ID=17261871

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP25421684A Granted JPS61132183A (ja)	1984-12-03	1984-12-03	サイクロデキストリン　グルカノトランスフエラ−ゼの製造法

Country Status (1)

Country	Link
JP (1)	JPS61132183A (de)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FR2574081A1 (fr) *	1984-12-03	1986-06-06	Hayashibara Biochem Lab	Polypeptide a activite de cyclomaltodextrine glucanotransferase
US5278059A (en) *	1985-12-04	1994-01-11	Kabushiki Kaisha Hayashibara Seibutsu Kagaki Kenkyujo	Polypeptide possessing cyclomaltodextrin glucanotransferase activity

1984
- 1984-12-03 JP JP25421684A patent/JPS61132183A/ja active Granted

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FR2574081A1 (fr) *	1984-12-03	1986-06-06	Hayashibara Biochem Lab	Polypeptide a activite de cyclomaltodextrine glucanotransferase
US5278059A (en) *	1985-12-04	1994-01-11	Kabushiki Kaisha Hayashibara Seibutsu Kagaki Kenkyujo	Polypeptide possessing cyclomaltodextrin glucanotransferase activity

Also Published As

Publication number	Publication date
JPH0355107B2 (de)	1991-08-22

Legal Events

Date	Code	Title	Description
2004-12-03	EXPY	Cancellation because of completion of term

Publication	Publication Date	Title
Surzycki	1969	Genetic functions of the chloroplast of Chlamydomonas reinhardi: effect of rifampin on chloroplast DNA-dependent RNA polymerase
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CA2183632A1 (en)	1995-08-31	Process for producing 2-keto-l-gulonic acid
US4656136A (en)	1987-04-07	Method for producing L-aspartic acid
US4692409A (en)	1987-09-08	Method for producing L-aspartic acid
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EP0462674A1 (de)	1991-12-27	Ein von P. chrysogenum erhältliches Oxidreduktasesystem, Gene die dafür kodieren und deren Verwendung zur Steigerung der Antibiotikaproduktion
US5049501A (en)	1991-09-17	Production method for PvuI restriction endonuclease
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JP2712331B2 (ja)	1998-02-10	アシルアミノ酸ラセマーゼ、その製造法および用途
JPS6251987A (ja)	1987-03-06	サイクロデキストリン　グルカノトランスフエラ−ゼの製造法
JPH0898683A (ja)	1996-04-16	７−アミノセファロスポラン酸エステラーゼ
JPH0262237B2 (de)	1990-12-25
JP2928884B2 (ja)	1999-08-03	Ｄｎａおよびその用途
EP0394479A1 (de)	1990-10-31	Gen, kodierend für tyrosin-phenol-lyase und dessen verwendung
JPS61132178A (ja)	1986-06-19	変異バチルス・ズブチリス
JPH04117281A (ja)	1992-04-17	ベタインアルデヒド脱水素酵素の製造方法
JP3026111B2 (ja)	2000-03-27	新規アルカリプロテアーゼ及びその製造方法
JPH0367580A (ja)	1991-03-22	ストレプトミセスｓｐ．Ｙ―１８３及びヒダントイナーゼの製造方法
JPH04349890A (ja)	1992-12-04	モノアセチルポリアミンの製造方法
JPH03224483A (ja)	1991-10-03	Ｌ―アミノアシラーゼａ

JPS61132183A - サイクロデキストリン グルカノトランスフエラ−ゼの製造法 - Google Patents

Info

Links

Classifications

Landscapes

Priority Applications (1)

Applications Claiming Priority (1)

Publications (2)

Family

ID=17261871

Family Applications (1)

Country Status (1)

Cited By (2)

Cited By (2)

Also Published As

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Legal Events

JPS61132183A - サイクロデキストリン　グルカノトランスフエラ−ゼの製造法 - Google Patents