MA45531A - Procédé de criblage d'un mutant dans une population d'organismes par application d'une approche de regroupement et de division - Google Patents

Procédé de criblage d'un mutant dans une population d'organismes par application d'une approche de regroupement et de division

Info

Publication number
MA45531A
MA45531A MA045531A MA45531A MA45531A MA 45531 A MA45531 A MA 45531A MA 045531 A MA045531 A MA 045531A MA 45531 A MA45531 A MA 45531A MA 45531 A MA45531 A MA 45531A
Authority
MA
Morocco
Prior art keywords
mutant
grouping
organisms
population
screening
Prior art date
Application number
MA045531A
Other languages
English (en)
Inventor
Massimiliano Carciofi
Søren Knudsen
Ole Olsen
Magnus Wohlfahrt Rasmussen
Birgitte Skadhauge
Alexander Striebeck
Hanne Cecilie Thomsen
Toni Wendt
Original Assignee
Carlsberg As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carlsberg As filed Critical Carlsberg As
Publication of MA45531A publication Critical patent/MA45531A/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4624—Hordeum vulgarus [barley]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
MA045531A 2016-07-01 2017-06-23 Procédé de criblage d'un mutant dans une population d'organismes par application d'une approche de regroupement et de division MA45531A (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DKPA201670485 2016-07-01

Publications (1)

Publication Number Publication Date
MA45531A true MA45531A (fr) 2019-05-08

Family

ID=56681913

Family Applications (3)

Application Number Title Priority Date Filing Date
MA052594A MA52594A (fr) 2016-07-01 2017-06-23 Procédé d'identification d'un sous-ensemble comprenant un organisme mutant
MA045531A MA45531A (fr) 2016-07-01 2017-06-23 Procédé de criblage d'un mutant dans une population d'organismes par application d'une approche de regroupement et de division
MA054568A MA54568A (fr) 2016-07-01 2017-06-23 Substitutions de nucléotides prédéterminées

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MA052594A MA52594A (fr) 2016-07-01 2017-06-23 Procédé d'identification d'un sous-ensemble comprenant un organisme mutant

Family Applications After (1)

Application Number Title Priority Date Filing Date
MA054568A MA54568A (fr) 2016-07-01 2017-06-23 Substitutions de nucléotides prédéterminées

Country Status (24)

Country Link
US (2) US11884972B2 (fr)
EP (3) EP3693458A1 (fr)
JP (2) JP7208797B2 (fr)
CN (2) CN114959098A (fr)
AR (1) AR110103A1 (fr)
AU (3) AU2017288960B2 (fr)
CA (1) CA3026016A1 (fr)
CL (1) CL2018003710A1 (fr)
CO (1) CO2019000828A2 (fr)
CR (1) CR20190040A (fr)
CU (1) CU20180158A7 (fr)
DK (1) DK3478832T3 (fr)
EA (1) EA201892809A1 (fr)
ES (2) ES2916221T3 (fr)
IL (1) IL263451B (fr)
MA (3) MA52594A (fr)
MX (2) MX2018016110A (fr)
PE (1) PE20190228A1 (fr)
PL (2) PL3478832T3 (fr)
PT (1) PT3478832T (fr)
SG (1) SG11201811021YA (fr)
UY (1) UY37312A (fr)
WO (1) WO2018001884A1 (fr)
ZA (3) ZA201808218B (fr)

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CN114959098A (zh) * 2016-07-01 2022-08-30 嘉士伯有限公司 通过应用混合分裂方法筛选生物群体内突变体的方法
MA51410A (fr) 2017-12-28 2021-04-07 Carlsberg As Plantes céréalières présentant des propriétés de paroi cellulaire améliorées
SG11202100302XA (en) * 2018-07-13 2021-02-25 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
US20220362771A1 (en) * 2018-07-13 2022-11-17 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
CN108913698B (zh) * 2018-07-25 2021-10-26 安徽农业大学 一种与小麦穗发芽抗性/感性相关的caps标记及其应用
CN110724741B (zh) * 2019-07-24 2020-05-19 艾普拜生物科技(苏州)有限公司 一种检测微小残留白血病相关融合基因的引物、探针及其试剂盒
CN114144509A (zh) 2019-08-16 2022-03-04 喜力供应链有限公司 无醇饮料的生产
EP3785529A1 (fr) 2019-08-28 2021-03-03 Carlsberg A/S Plantes d'orge avec une teneur en protéine modifiéé dans les graines
EP4040946A1 (fr) 2019-10-10 2022-08-17 Carlsberg A/S Procédés de préparation de compositions de plantes mutantes
US20240043911A1 (en) * 2020-02-06 2024-02-08 The Administrators Of The Tulane Educational Fund Crispr-based assay for detecting tb in bodily fluids
MX2022010777A (es) 2020-03-02 2022-11-30 Carlsberg As Plantas de cebada con elevada actividad de dextrinasa limite.
EP4029935A1 (fr) 2021-01-14 2022-07-20 Carlsberg A/S Souche de s. eubayanus et ses hybrides
WO2023076908A1 (fr) * 2021-10-25 2023-05-04 Gt Molecular, Inc. Séquences de blocage hors cible pour améliorer la distinction de la cible par réaction en chaîne de la polymérase
US20250051793A1 (en) 2021-12-22 2025-02-13 BASF Agricultural Solutions Seed US LLC Improved screening method for genome edited events
AR128756A1 (es) 2022-03-11 2024-06-12 Carlsberg As Modulación de niveles de proteínas
EP4618742A1 (fr) 2022-11-16 2025-09-24 University of Copenhagen Plantes à faible teneur en glucosinolates dans les graines
WO2024133900A1 (fr) 2022-12-22 2024-06-27 University Of Copenhagen Production de (-)-spartéine améliorée
AR132565A1 (es) 2023-05-01 2025-07-16 Carlsberg As Cebada con propiedades mejoradas
WO2025108936A1 (fr) * 2023-11-20 2025-05-30 Eth Zurich Procédé de fourniture de cellules génétiquement modifiées
CN117965576B (zh) * 2024-02-01 2024-08-27 北京林业大学 一种王百合苷生物合成基因LlHCT1及其应用
EP4644572A1 (fr) * 2024-04-29 2025-11-05 KWS SAAT SE & Co. KGaA Procédé d'analyse de pureté de caractères dans des grands volumes de semences
WO2025240938A2 (fr) * 2024-05-16 2025-11-20 Oath, Inc. Production de consortiums microbiens
WO2026037962A1 (fr) 2024-08-15 2026-02-19 University Of Copenhagen Légumineuses ou parties de celles-ci à teneur réduite en alcaloïdes de quinolizidine

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EP4040946A1 (fr) * 2019-10-10 2022-08-17 Carlsberg A/S Procédés de préparation de compositions de plantes mutantes

Also Published As

Publication number Publication date
AU2019268102B2 (en) 2020-03-05
EA201892809A1 (ru) 2019-06-28
US20240182961A1 (en) 2024-06-06
ZA201907775B (en) 2022-09-28
PT3478832T (pt) 2022-05-19
AR110103A1 (es) 2019-02-27
ES3032697T3 (en) 2025-07-23
JP2022101703A (ja) 2022-07-06
ZA201808218B (en) 2020-02-26
MX2022014965A (es) 2023-01-11
JP7539428B2 (ja) 2024-08-23
BR112018076429A2 (pt) 2019-10-01
JP7208797B2 (ja) 2023-01-19
UY37312A (es) 2018-02-28
US20190194723A1 (en) 2019-06-27
CN109477087A (zh) 2019-03-15
CO2019000828A2 (es) 2019-04-30
NZ749382A (en) 2025-06-27
IL263451A (en) 2019-01-31
ES2916221T3 (es) 2022-06-29
JP2019524083A (ja) 2019-09-05
AU2017288960B2 (en) 2019-12-12
SG11201811021YA (en) 2019-01-30
IL263451B (en) 2022-08-01
AU2019268103B2 (en) 2020-03-12
CL2018003710A1 (es) 2019-03-22
ZA201907774B (en) 2022-07-27
WO2018001884A1 (fr) 2018-01-04
EP3792357A1 (fr) 2021-03-17
US12428678B2 (en) 2025-09-30
AU2019268102A1 (en) 2019-12-12
AU2019268103A1 (en) 2019-12-12
EP3693458A1 (fr) 2020-08-12
EP3792357B1 (fr) 2025-05-07
CA3026016A1 (fr) 2018-01-04
AU2017288960A1 (en) 2019-01-17
DK3478832T3 (da) 2022-05-30
MX2018016110A (es) 2019-05-30
PL3792357T3 (pl) 2025-07-07
EP3478832B1 (fr) 2022-02-23
PL3478832T3 (pl) 2022-06-06
MA52594A (fr) 2021-03-17
US11884972B2 (en) 2024-01-30
MA54568A (fr) 2021-10-27
EP3792357C0 (fr) 2025-05-07
CU20180158A7 (es) 2019-08-06
CN109477087B (zh) 2022-06-24
PE20190228A1 (es) 2019-02-13
CR20190040A (es) 2019-05-09
CN114959098A (zh) 2022-08-30
EP3478832A1 (fr) 2019-05-08

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