CU20180158A7 - Método para identificar un mutante dentro de una población de organismos aplicando un enfoque de conjunto y división - Google Patents

Método para identificar un mutante dentro de una población de organismos aplicando un enfoque de conjunto y división

Info

Publication number
CU20180158A7
CU20180158A7 CU2018000158A CU20180158A CU20180158A7 CU 20180158 A7 CU20180158 A7 CU 20180158A7 CU 2018000158 A CU2018000158 A CU 2018000158A CU 20180158 A CU20180158 A CU 20180158A CU 20180158 A7 CU20180158 A7 CU 20180158A7
Authority
CU
Cuba
Prior art keywords
organisms
dpcr
finding
mutant
population
Prior art date
Application number
CU2018000158A
Other languages
English (en)
Inventor
Massimiliano Carciofi
Søren Knudsen
Ole Oslen
Magus Wohlfahrt Rasmessen
Birgitte Skadhauge
Alexander Striebeck
Hanne Cecilie Thomsen
Toni Wendt
Original Assignee
Carlsberg As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carlsberg As filed Critical Carlsberg As
Publication of CU20180158A7 publication Critical patent/CU20180158A7/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4624—Hordeum vulgarus [barley]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

<p>En los enfoques de mejoramiento de plantas tradicionales, puede utilizarse mutagénesis química para introducir sustituciones nucleotídicas aleatoriamente en el genoma de una planta, es decir sin posibilidades de controlar los sitios de los cambios nucleotídicos. A causa de las complejidades genómicas, la probabilidad estadística es extremadamente baja en cuanto a hallar una sustitución nucleotídica predeterminada. La presente invención, sin embargo, demuestra cómo se desarrolla un uso novedoso, alternativo, de la reacción en cadena de la polimerasa digital (dPCR), preferiblemente dPCR en gotas (ddPCR), para explotar el hallazgo de sustituciones nucleotídicas específicas en genes mutados. La plataforma completa comprende un método de relevamiento con una biblioteca de organismos mutagenizados, sistemas basados en PCR digital y una configuración para propagar y analizar organismos mutados identificados.</p>
CU2018000158A 2016-07-01 2017-06-23 Método para identificar un mutante dentro de una población de organismos aplicando un enfoque de conjunto y división CU20180158A7 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA201670485 2016-07-01
PCT/EP2017/065516 WO2018001884A1 (en) 2016-07-01 2017-06-23 Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach

Publications (1)

Publication Number Publication Date
CU20180158A7 true CU20180158A7 (es) 2019-08-06

Family

ID=56681913

Family Applications (1)

Application Number Title Priority Date Filing Date
CU2018000158A CU20180158A7 (es) 2016-07-01 2017-06-23 Método para identificar un mutante dentro de una población de organismos aplicando un enfoque de conjunto y división

Country Status (24)

Country Link
US (2) US11884972B2 (es)
EP (3) EP3792357B1 (es)
JP (2) JP7208797B2 (es)
CN (2) CN109477087B (es)
AR (1) AR110103A1 (es)
AU (3) AU2017288960B2 (es)
CA (1) CA3026016A1 (es)
CL (1) CL2018003710A1 (es)
CO (1) CO2019000828A2 (es)
CR (1) CR20190040A (es)
CU (1) CU20180158A7 (es)
DK (1) DK3478832T3 (es)
EA (1) EA201892809A1 (es)
ES (2) ES2916221T3 (es)
IL (1) IL263451B (es)
MA (3) MA45531A (es)
MX (2) MX2018016110A (es)
PE (1) PE20190228A1 (es)
PL (2) PL3478832T3 (es)
PT (1) PT3478832T (es)
SG (1) SG11201811021YA (es)
UY (1) UY37312A (es)
WO (1) WO2018001884A1 (es)
ZA (3) ZA201808218B (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL3478832T3 (pl) * 2016-07-01 2022-06-06 Carlsberg A/S Sposób skriningu populacji organizmów pod kątem mutanta poprzez zastosowanie podejścia polegającego na łączeniu i dzieleniu
US11690341B2 (en) 2017-12-28 2023-07-04 Carlsberg A/S Cereal plants with improved cell wall properties
US20220362771A1 (en) * 2018-07-13 2022-11-17 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
WO2020012004A1 (en) * 2018-07-13 2020-01-16 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
CN108913698B (zh) * 2018-07-25 2021-10-26 安徽农业大学 一种与小麦穗发芽抗性/感性相关的caps标记及其应用
CN110724741B (zh) * 2019-07-24 2020-05-19 艾普拜生物科技(苏州)有限公司 一种检测微小残留白血病相关融合基因的引物、探针及其试剂盒
EP4013843A1 (en) 2019-08-16 2022-06-22 Heineken Supply Chain B.V. Production of an alcohol-free beverage
EP3785529A1 (en) 2019-08-28 2021-03-03 Carlsberg A/S Barley plants with altered protein content in grains
MX2022004361A (es) 2019-10-10 2022-05-03 Carlsberg As Metodos para preparar plantas mutantes.
US20240043911A1 (en) * 2020-02-06 2024-02-08 The Administrators Of The Tulane Educational Fund Crispr-based assay for detecting tb in bodily fluids
WO2021175786A1 (en) 2020-03-02 2021-09-10 Carlsberg A/S Barley plants with high limit dextrinase activity
EP4029935A1 (en) 2021-01-14 2022-07-20 Carlsberg A/S S. eubayanus strain and hybrids thereof
US20230250467A1 (en) * 2021-10-25 2023-08-10 Gt Molecular, Inc. Off-target blocking sequences to improve target discrimination by polymerase chain reaction
EP4451853A1 (en) 2021-12-22 2024-10-30 Basf Agricultural Solutions Seed Us Llc Improved screening method for genome edited events
AR128756A1 (es) 2022-03-11 2024-06-12 Carlsberg As Modulación de niveles de proteínas
AU2023379942A1 (en) 2022-11-16 2025-04-10 University Of Copenhagen Plants with low seed glucosinolate content
WO2024133900A1 (en) 2022-12-22 2024-06-27 University Of Copenhagen Improved (-)-sparteine production
WO2024227748A1 (en) 2023-05-01 2024-11-07 Carlsberg A/S Barley with improved properties
WO2025108936A1 (en) * 2023-11-20 2025-05-30 Eth Zurich Method for providing genetically-modified cells
CN117965576B (zh) * 2024-02-01 2024-08-27 北京林业大学 一种王百合苷生物合成基因LlHCT1及其应用
EP4644572A1 (en) * 2024-04-29 2025-11-05 KWS SAAT SE & Co. KGaA Method for analysing trait purity in large seed bulks
WO2025240938A2 (en) * 2024-05-16 2025-11-20 Oath, Inc. Microbial consortia production
WO2026037962A1 (en) 2024-08-15 2026-02-19 University Of Copenhagen Legumes or parts thereof with reduced quinolizidine alkaloid content

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
KR20000057258A (ko) * 1996-11-26 2000-09-15 크로커 사무엘 에스 염색체 말단으로 텔로머라제가 접근하는 것을 방지하는 억제제의 확인
CA2288988A1 (en) 1997-06-04 1998-12-10 Smithkline Beecham Corporation Methods for rapid cloning of full length cdnas
IL138800A0 (en) 1998-04-02 2001-10-31 Tellus Genetic Resources Inc A method of identifying a plant with a lesion in a gene sequence
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
AU2002314417A1 (en) * 2001-06-22 2003-01-08 Syngenta Participations Ag Identification and characterization of plant genes
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7420105B2 (en) 2004-03-11 2008-09-02 Carlsberg A/S Barley for production of flavor-stable beverage
CA2738578C (en) 2008-09-23 2021-05-04 Quantalife, Inc. Droplet-based assay system
US9005565B2 (en) 2010-06-24 2015-04-14 Hamid-Reza Jahangiri-Famenini Method and apparatus for forming graphene
EP3498864B1 (en) * 2011-03-10 2024-10-09 Gen-Probe Incorporated Methods for the selection and optimization of oligonucleotide tag sequences
CA2831180C (en) 2011-04-11 2017-02-14 Keith Bauer Dna polymerases with improved activity
EP3971283A1 (en) 2014-02-27 2022-03-23 Monsanto Technology LLC Compositions and methods for site directed genomic modification
GB201411603D0 (en) * 2014-06-30 2014-08-13 Vela Operations Pte Ltd Compositions for quantitative and/or semiquantitative mutation detection methods
PL3478832T3 (pl) * 2016-07-01 2022-06-06 Carlsberg A/S Sposób skriningu populacji organizmów pod kątem mutanta poprzez zastosowanie podejścia polegającego na łączeniu i dzieleniu
MX2022004361A (es) * 2019-10-10 2022-05-03 Carlsberg As Metodos para preparar plantas mutantes.

Also Published As

Publication number Publication date
JP2022101703A (ja) 2022-07-06
MA45531A (fr) 2019-05-08
UY37312A (es) 2018-02-28
ZA201907775B (en) 2022-09-28
CN114959098A (zh) 2022-08-30
AR110103A1 (es) 2019-02-27
EP3792357B1 (en) 2025-05-07
PL3478832T3 (pl) 2022-06-06
US11884972B2 (en) 2024-01-30
CA3026016A1 (en) 2018-01-04
CL2018003710A1 (es) 2019-03-22
BR112018076429A2 (pt) 2019-10-01
EP3478832B1 (en) 2022-02-23
SG11201811021YA (en) 2019-01-30
PE20190228A1 (es) 2019-02-13
US20240182961A1 (en) 2024-06-06
EA201892809A1 (ru) 2019-06-28
CO2019000828A2 (es) 2019-04-30
AU2017288960B2 (en) 2019-12-12
AU2019268103A1 (en) 2019-12-12
ZA201907774B (en) 2022-07-27
MX2018016110A (es) 2019-05-30
PL3792357T3 (pl) 2025-07-07
AU2019268102B2 (en) 2020-03-05
EP3693458A1 (en) 2020-08-12
EP3478832A1 (en) 2019-05-08
AU2019268103B2 (en) 2020-03-12
JP7539428B2 (ja) 2024-08-23
US20190194723A1 (en) 2019-06-27
NZ749382A (en) 2025-06-27
JP7208797B2 (ja) 2023-01-19
ZA201808218B (en) 2020-02-26
ES3032697T3 (en) 2025-07-23
IL263451A (en) 2019-01-31
MA54568A (fr) 2021-10-27
CN109477087A (zh) 2019-03-15
PT3478832T (pt) 2022-05-19
EP3792357C0 (en) 2025-05-07
CN109477087B (zh) 2022-06-24
MA52594A (fr) 2021-03-17
ES2916221T3 (es) 2022-06-29
AU2019268102A1 (en) 2019-12-12
MX2022014965A (es) 2023-01-11
DK3478832T3 (da) 2022-05-30
WO2018001884A1 (en) 2018-01-04
JP2019524083A (ja) 2019-09-05
AU2017288960A1 (en) 2019-01-17
EP3792357A1 (en) 2021-03-17
CR20190040A (es) 2019-05-09
IL263451B (en) 2022-08-01
US12428678B2 (en) 2025-09-30

Similar Documents

Publication Publication Date Title
CO2019000828A2 (es) Método para detectar un mutante dentro de una población de organismos aplicando el enfoque de agrupación y división
Albertsen et al. Back to basics–the influence of DNA extraction and primer choice on phylogenetic analysis of activated sludge communities
Keeling et al. The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing
Liang et al. Single-cell sequencing technologies: current and future
MX2017005306A (es) Novedosos sitios de integracion en celulas cho y usos de estos.
EP4424824A3 (en) Nucleic acid-based data storage
BR112016019267B8 (pt) Método para construir um perfil de dna, biblioteca de ácido nucléico, método para construir uma biblioteca de ácido nucléico, pluralidade de iniciadores e kit
MX2017003676A (es) Metodos para reproducir plantas asexualmente y composiciones de las mismas.
AR101943A1 (es) Métodos para detección de patógenos y control de enfermedades en carnes, plantas o partes de plantas
EP4410978A3 (en) Methods for quantitative genetic analysis of cell free dna
MX2017001405A (es) Deteccion de acidos nucleicos diana mediante el uso de hibridacion.
WO2014138119A3 (en) Method and kit for characterizing microorganisms
TR201903963T4 (tr) Hizalama içermeyen sekans analizi kullanılarak bir hedef sekans içindeki mutasyon(lar)ı içeren üyeler için bir popülasyonun taranmasına ait yüksek verimlilik metodu.
EP4512527A3 (en) Microfluidic siphoning array for nucleic acid quantification
EP3795681A3 (en) Polymerase compositions and methods of making and using same
EA202192652A1 (ru) Способ конструирования химерной плазмидной библиотеки
DiCarlo et al. RNA-guided gene drives can efficiently and reversibly bias inheritance in wild yeast
Panzera et al. Phylogenomic analysis of the Chilean clade of Liolaemus lizards (Squamata: Liolaemidae) based on sequence capture data
BR112017006542A2 (pt) mutantes de recombinase
EA202190758A1 (ru) Композиция для индукции гибели клеток, имеющих мутированный ген, и способ индукции гибели клеток, имеющих мутированный ген, с применением композиции
EP4012049A3 (en) Methods for determining responsiveness to mek/erk inhibitors
AR110615A1 (es) Gen alkb mutante
CL2019003825A1 (es) Método de distinguir entre alelos tipo salvaje y mutantes de un polimorfismo de nucleótido único de piscirickettsia salmonis (snp); sonda de ácido nucleico; par de cebadores de pcr. (divisional solicitud 201702982)
Schmidt et al. Cell lineage tracing using nuclease barcoding
Mugal et al. Measures of natural selection in protein coding genes