NO308667B1 - FremgangsmÕte for fremstilling av fusjonsproteiner - Google Patents

FremgangsmÕte for fremstilling av fusjonsproteiner Download PDF

Info

Publication number: NO308667B1
Authority: NO; Norway
Prior art keywords: plasmid; oligonucleotide; gene; sequence; protein
Prior art date: 1989-08-29

Application number

NO920774A

Other languages

English (en)

Norwegian (no)

Other versions

NO920774D0 (no

NO920774L (no

Inventor

Siegfried Stengelin

Wolfgang Ulmer

Paul Habermann

Eugen Uhlmann

Brian Seed

Original Assignee

Hoechst Ag

Gen Hospital Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-08-29

Filing date

1992-02-27

Publication date

2000-10-09

1992-02-27 Application filed by Hoechst Ag, Gen Hospital Corp filed Critical Hoechst Ag

1992-02-27 Publication of NO920774D0 publication Critical patent/NO920774D0/no

1992-04-28 Publication of NO920774L publication Critical patent/NO920774L/no

2000-10-09 Publication of NO308667B1 publication Critical patent/NO308667B1/no

Links

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PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical group C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 description 1
239000004026 insulin derivative Substances 0.000 description 1
210000000936 intestine Anatomy 0.000 description 1
BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
229960000274 lysozyme Drugs 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
230000035772 mutation Effects 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
108040007629 peroxidase activity proteins Proteins 0.000 description 1
238000000734 protein sequencing Methods 0.000 description 1
239000013558 reference substance Substances 0.000 description 1
230000010076 replication Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
238000004007 reversed phase HPLC Methods 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
239000007974 sodium acetate buffer Substances 0.000 description 1
235000010265 sodium sulphite Nutrition 0.000 description 1
HAEPBEMBOAIUPN-UHFFFAOYSA-L sodium tetrathionate Chemical compound O.O.[Na+].[Na+].[O-]S(=O)(=O)SSS([O-])(=O)=O HAEPBEMBOAIUPN-UHFFFAOYSA-L 0.000 description 1
239000007787 solid Substances 0.000 description 1
238000005063 solubilization Methods 0.000 description 1
230000007928 solubilization Effects 0.000 description 1
NHXLMOGPVYXJNR-ATOGVRKGSA-N somatostatin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CSSC[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C3=CC=CC=C3NC=2)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N1)[C@@H](C)O)NC(=O)CNC(=O)[C@H](C)N)C(O)=O)=O)[C@H](O)C)C1=CC=CC=C1 NHXLMOGPVYXJNR-ATOGVRKGSA-N 0.000 description 1
229960000553 somatostatin Drugs 0.000 description 1
238000010186 staining Methods 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000007858 starting material Substances 0.000 description 1
238000012360 testing method Methods 0.000 description 1
230000014616 translation Effects 0.000 description 1
239000012588 trypsin Substances 0.000 description 1
239000002753 trypsin inhibitor Substances 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
238000005406 washing Methods 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
210000005253 yeast cell Anatomy 0.000 description 1
239000011592 zinc chloride Substances 0.000 description 1
235000005074 zinc chloride Nutrition 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Gastroenterology & Hepatology (AREA)
Toxicology (AREA)
Plant Pathology (AREA)
Endocrinology (AREA)
Medicinal Chemistry (AREA)
Diabetes (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

NO920774A 1989-08-29 1992-02-27 FremgangsmÕte for fremstilling av fusjonsproteiner NO308667B1 (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US39987489A	1989-08-29	1989-08-29
PCT/US1990/004840 WO1991003550A1 (fr)	1989-08-29	1990-08-28	Proteines de fusion, leur preparation et utilisation

Publications (3)

Publication Number	Publication Date
NO920774D0 NO920774D0 (no)	1992-02-27
NO920774L NO920774L (no)	1992-04-28
NO308667B1 true NO308667B1 (no)	2000-10-09

Family

ID=23581315

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO920774A NO308667B1 (no)	1989-08-29	1992-02-27	FremgangsmÕte for fremstilling av fusjonsproteiner

Country Status (19)

Country	Link
EP (1)	EP0489780B1 (fr)
JP (1)	JP3043803B2 (fr)
KR (1)	KR0159786B1 (fr)
AT (1)	ATE173018T1 (fr)
AU (1)	AU638277B2 (fr)
CA (1)	CA2065146C (fr)
CY (1)	CY2168B1 (fr)
DE (1)	DE69032743T2 (fr)
DK (1)	DK0489780T3 (fr)
ES (1)	ES2124216T3 (fr)
FI (1)	FI113183B (fr)
GR (1)	GR1005153B (fr)
HU (1)	HU216069B (fr)
IE (1)	IE903120A1 (fr)
IL (1)	IL95495A (fr)
NO (1)	NO308667B1 (fr)
PT (1)	PT95111B (fr)
WO (1)	WO1991003550A1 (fr)
ZA (1)	ZA906839B (fr)

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DK0622376T3 (da) *	1993-04-27	2001-11-12	Hoechst Ag	Amorfe monosfæriske former af insulinderivater
WO1995009914A1 (fr) *	1993-10-05	1995-04-13	Asahi Glass Company Ltd.	Vecteur de multiclonage, vecteur d'expression, et production d'une proteine exogene au moyen dudit vecteur d'expression
US6001604A (en) *	1993-12-29	1999-12-14	Bio-Technology General Corp.	Refolding of proinsulins without addition of reducing agents
DE4405179A1 (de) *	1994-02-18	1995-08-24	Hoechst Ag	Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
US5683987A (en) *	1994-07-12	1997-11-04	The Board Of Regents Of The University Of Nebraska	Therapeutic oligonucleotides targeting the human MDR1 and MRP genes
DK0821006T3 (da)	1996-07-26	2004-08-16	Aventis Pharma Gmbh	Insulinderivater med öget zinkbinding
DE19735711C2 (de)	1997-08-18	2001-04-26	Aventis Pharma Gmbh	Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
DE19825447A1 (de)	1998-06-06	1999-12-09	Hoechst Marion Roussel De Gmbh	Neue Insulinanaloga mit erhöhter Zinkbildung
DE19915938A1 (de)	1999-04-09	2000-10-19	Aventis Pharma Gmbh	Herstellung von pankreatischer Procarboxypeptidase B, Isoformen und Muteinen davon und ihre Verwendung
DE19930676B4 (de)	1999-07-02	2006-01-19	Sanofi-Aventis Deutschland Gmbh	Verfahren zur Stabilisierung von Insulin, Insulinderivaten und/oder deren Vorläufer in komplexen Mischungen bei deren Lagerung in wäßrigen Lösungsmitteln
US7202059B2 (en)	2001-02-20	2007-04-10	Sanofi-Aventis Deutschland Gmbh	Fusion proteins capable of being secreted into a fermentation medium
US7638618B2 (en)	2001-02-20	2009-12-29	Sanofi-Aventis Deutschland Gmbh	Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
DE10235168A1 (de)	2002-08-01	2004-02-12	Aventis Pharma Deutschland Gmbh	Verfahren zur Reinigung von Preproinsulin
US20060035316A1 (en) *	2002-11-12	2006-02-16	Sang-Yong Lee	Plasmids expressing human insulin and the preparation method for human insuling thereby
DE102008025007A1 (de)	2008-05-24	2009-11-26	Sanofi-Aventis Deutschland Gmbh	Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
CN102007143B (zh)	2008-01-09	2015-08-26	塞诺菲-安万特德国有限公司	具有超延迟时效特征的新型胰岛素衍生物
DE102008003568A1 (de)	2008-01-09	2009-07-16	Sanofi-Aventis Deutschland Gmbh	Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
DE102008003566A1 (de)	2008-01-09	2009-07-16	Sanofi-Aventis Deutschland Gmbh	Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
EP2229406B1 (fr)	2008-01-09	2015-04-22	Sanofi-Aventis Deutschland GmbH	Nouveaux dérivés d'insuline à profil temporel/d'action extrêmement retardé
DE102008025008A1 (de)	2008-05-24	2009-11-26	Sanofi-Aventis Deutschland Gmbh	Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
CN102256618A (zh)	2008-10-17	2011-11-23	赛诺菲-安万特德国有限公司	胰岛素和glp-1激动剂的组合
PL2451437T3 (pl)	2009-07-06	2017-05-31	Sanofi-Aventis Deutschland Gmbh	Wodne preparaty insuliny zawierające metioninę
TR201809460T4 (tr)	2009-11-13	2018-07-23	Sanofi Aventis Deutschland	Bir GLP- 1-agonisti, bir insülin ve metiyonin içeren farmasötik bileşim.
NZ599847A (en)	2009-11-13	2013-09-27	Sanofi Aventis Deutschland	Pharmaceutical composition comprising a glp-1 agonist and methionine
WO2012028172A1 (fr)	2010-08-30	2012-03-08	Sanofi-Aventis Deutschland Gmbh	Utilisation de lixisenatide pour fabrication de médicament pour traitement du diabète sucré de type 2
DK2739646T3 (en)	2011-05-10	2018-02-26	Stefan Hermann	COMPREHENSIVE BUFFER SYSTEM FOR RENATURING HUMAN PROINSULIN OR PROINSULIN DERIVATIVES
US9821032B2 (en)	2011-05-13	2017-11-21	Sanofi-Aventis Deutschland Gmbh	Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
AR087693A1 (es)	2011-08-29	2014-04-09	Sanofi Aventis Deutschland	Combinacion farmaceutica para uso en el control glucemico en pacientes con diabetes de tipo 2
TWI559929B (en)	2011-09-01	2016-12-01	Sanofi Aventis Deutschland	Pharmaceutical composition for use in the treatment of a neurodegenerative disease
JP5858945B2 (ja)	2012-03-15	2016-02-10	アークレイ株式会社	酵素を用いた測定方法
PE20171622A1 (es)	2014-12-12	2017-11-02	Sanofi Aventis Deutschland	Formulacion de relacion fija de insulina glargina/lixisenatida
TWI748945B (zh)	2015-03-13	2021-12-11	德商賽諾菲阿凡提斯德意志有限公司	第２型糖尿病病患治療
TW201705975A (zh)	2015-03-18	2017-02-16	賽諾菲阿凡提斯德意志有限公司	第２型糖尿病病患之治療
JP6976254B2 (ja) *	2015-09-18	2021-12-08	バイオネットファーマゲゼルシャフトミットベシュレンクテルハフツングBioNet Pharma GmbH	Ｃｄ−ｒａｐ前駆体タンパク質の使用による、ｃｄ−ｒａｐの製造方法における発現およびフォールディングの改善
JP7460182B2 (ja) *	2019-02-19	2024-04-02	ザ、トラスティーズオブプリンストンユニバーシティ	フォールディングされたドメインおよびｒｎａ顆粒会合タンパク質ドメインの光制御オリゴマー化および相分離のためのシステムおよび方法

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EP0195680A3 (fr) *	1985-03-21	1987-06-10	Immunex Corporation	Synthèse de protéines avec un peptide d'identification
DE3526995A1 (de) *	1985-07-27	1987-02-05	Hoechst Ag	Fusionsproteine, verfahren zu ihrer herstellung und ihre verwendung

1990
- 1990-08-27 IL IL9549590A patent/IL95495A/en active IP Right Grant
- 1990-08-27 GR GR900100635A patent/GR1005153B/el not_active IP Right Cessation
- 1990-08-28 IE IE312090A patent/IE903120A1/en not_active IP Right Cessation
- 1990-08-28 DE DE69032743T patent/DE69032743T2/de not_active Expired - Lifetime
- 1990-08-28 DK DK90912715T patent/DK0489780T3/da active
- 1990-08-28 WO PCT/US1990/004840 patent/WO1991003550A1/fr not_active Ceased
- 1990-08-28 HU HUP9200674A patent/HU216069B/hu unknown
- 1990-08-28 CA CA002065146A patent/CA2065146C/fr not_active Expired - Lifetime
- 1990-08-28 ES ES90912715T patent/ES2124216T3/es not_active Expired - Lifetime
- 1990-08-28 KR KR1019920700456A patent/KR0159786B1/ko not_active Expired - Lifetime
- 1990-08-28 AU AU62872/90A patent/AU638277B2/en not_active Expired
- 1990-08-28 EP EP90912715A patent/EP0489780B1/fr not_active Expired - Lifetime
- 1990-08-28 JP JP2512297A patent/JP3043803B2/ja not_active Expired - Lifetime
- 1990-08-28 PT PT95111A patent/PT95111B/pt not_active IP Right Cessation
- 1990-08-28 AT AT90912715T patent/ATE173018T1/de not_active IP Right Cessation
- 1990-09-28 ZA ZA906839A patent/ZA906839B/xx unknown
1992
- 1992-02-27 NO NO920774A patent/NO308667B1/no not_active IP Right Cessation
- 1992-02-28 FI FI920923A patent/FI113183B/fi active
2000
- 2000-05-03 CY CY0000013A patent/CY2168B1/xx unknown

Also Published As

Publication number	Publication date
FI113183B (fi)	2004-03-15
JPH05501799A (ja)	1993-04-08
FI920923A0 (fi)	1992-02-28
DK0489780T3 (da)	1999-07-19
GR900100635A (el)	1991-12-30
GR1005153B (el)	2006-03-13
AU6287290A (en)	1991-04-08
ZA906839B (en)	1991-06-26
DE69032743D1 (de)	1998-12-10
AU638277B2 (en)	1993-06-24
JP3043803B2 (ja)	2000-05-22
PT95111A (pt)	1991-05-22
EP0489780B1 (fr)	1998-11-04
ES2124216T3 (es)	1999-02-01
HU216069B (hu)	1999-04-28
WO1991003550A1 (fr)	1991-03-21
HK1012026A1 (en)	1999-07-23
CY2168B1 (en)	2002-08-23
DE69032743T2 (de)	1999-06-17
EP0489780A1 (fr)	1992-06-17
EP0489780A4 (en)	1992-08-12
HUT60327A (en)	1992-08-28
KR0159786B1 (ko)	1998-11-16
IE903120A1 (en)	1991-03-13
HU9200674D0 (en)	1992-08-28
PT95111B (pt)	1998-10-30
ATE173018T1 (de)	1998-11-15
IL95495A0 (en)	1991-06-30
CA2065146C (fr)	2002-07-23
NO920774D0 (no)	1992-02-27
CA2065146A1 (fr)	1991-03-01
NO920774L (no)	1992-04-28
IL95495A (en)	1996-10-16

Legal Events

Date	Code	Title	Description
2010-09-13	MK1K	Patent expired

Publication	Publication Date	Title
NO308667B1 (no)	2000-10-09	FremgangsmÕte for fremstilling av fusjonsproteiner
US5227293A (en)	1993-07-13	Fusion proteins, their preparation and use
US5358857A (en)	1994-10-25	Method of preparing fusion proteins
JP2609367B2 (ja)	1997-05-14	プロセッシング部位に隣接する負に帯電したアミノ酸からなる酵母プロセッシングシステム
AU615760B2 (en)	1991-10-10	Insulin analogues
NO177009B (no)	1995-03-27	Analogifremgangsmåte ved fremstilling av insulinanaloger
JP4855640B2 (ja)	2012-01-18	インシュリン前駆体及びその調製方法
US4826763A (en)	1989-05-02	Process for preparing glucagon or fragments or derivatives thereof in yeast
AU607973B2 (en)	1991-03-21	Process for production of physiologically active peptide containing cysteine residue
NO301122B1 (no)	1997-09-15	Rekombinant-metoder til produksjon av serinproteaseinhibitorer og DNA-sekvenser for disse
NO174717B (no)	1994-03-14	Fremgangsmaate for fremstilling av et fiskeveksthormon-polypeptid fra Oncorhynchus keta samt ekspresjonsvektorer til bruk ved fremgangsmaaten
CA2047119C (fr)	2002-10-08	Traitement in vitro de proteines de fusion
NO177967B (no)	1995-09-18	Fremgangsmåte ved fremstilling av aprotinin og aprotinin homologer i gjær, og en gjærstamme som anvendes i fremgangsmåten
FI97549C (fi)	1997-01-10	Menetelmä vierasproteiinien valmistamiseksi Streptomycessoluissa
WO2002079251A2 (fr)	2002-10-10	Methode de fabrication de precurseurs d'insuline humaine
HK1012026B (en)	2000-04-28	Fusion proteins, their preparation and use
WO2004085472A1 (fr)	2004-10-07	Procede de fabrication de precurseurs de l'insuline humaine et insuline humaine
US20050191728A1 (en)	2005-09-01	Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast