FI113183B - Menetelmä fuusioproteiinien valmistamiseksi - Google Patents

Menetelmä fuusioproteiinien valmistamiseksi Download PDF

Info

Publication number: FI113183B
Authority: FI; Finland
Prior art keywords: plasmid; oligonucleotide; gene; sequence; protein
Prior art date: 1989-08-29

Application number

FI920923A

Other languages

English (en)

Finnish (fi)

Swedish (sv)

Other versions

FI920923A0 (fi

Inventor

Paul Habermann

Eugen Uhlmann

Brian Seed

Wolfgang Ulmer

Siegfried Stengelin

Original Assignee

Gen Hospital Corp

Aventis Pharma Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-08-29

Filing date

1992-02-28

Publication date

2004-03-15

1992-02-28 Application filed by Gen Hospital Corp, Aventis Pharma Gmbh filed Critical Gen Hospital Corp

1992-02-28 Publication of FI920923A0 publication Critical patent/FI920923A0/fi

2004-03-15 Application granted granted Critical

2004-03-15 Publication of FI113183B publication Critical patent/FI113183B/fi

Links

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108090000631 Trypsin Proteins 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
101710162629 Trypsin inhibitor Proteins 0.000 description 1
229940122618 Trypsin inhibitor Drugs 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
239000011543 agarose gel Substances 0.000 description 1
238000013019 agitation Methods 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
108010072788 angiogenin Proteins 0.000 description 1
238000005571 anion exchange chromatography Methods 0.000 description 1
125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
235000010323 ascorbic acid Nutrition 0.000 description 1
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239000011668 ascorbic acid Substances 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
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238000003556 assay Methods 0.000 description 1
239000012131 assay buffer Substances 0.000 description 1
230000003115 biocidal effect Effects 0.000 description 1
244000309466 calf Species 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
108010004073 cysteinylcysteine Proteins 0.000 description 1
230000007423 decrease Effects 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
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239000000499 gel Substances 0.000 description 1
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SYKWLIJQEHRDNH-CKRMAKSASA-N glutaryl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 SYKWLIJQEHRDNH-CKRMAKSASA-N 0.000 description 1
230000014726 immortalization of host cell Effects 0.000 description 1
238000003119 immunoblot Methods 0.000 description 1
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239000004615 ingredient Substances 0.000 description 1
238000003780 insertion Methods 0.000 description 1
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PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical group C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 description 1
210000000936 intestine Anatomy 0.000 description 1
BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
101150117702 lpl3 gene Proteins 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
230000035772 mutation Effects 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
230000003389 potentiating effect Effects 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
229940002612 prodrug Drugs 0.000 description 1
239000000651 prodrug Substances 0.000 description 1
238000000734 protein sequencing Methods 0.000 description 1
239000013558 reference substance Substances 0.000 description 1
230000010076 replication Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
238000004007 reversed phase HPLC Methods 0.000 description 1
235000019515 salmon Nutrition 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000010187 selection method Methods 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
235000010265 sodium sulphite Nutrition 0.000 description 1
HAEPBEMBOAIUPN-UHFFFAOYSA-L sodium tetrathionate Chemical compound O.O.[Na+].[Na+].[O-]S(=O)(=O)SSS([O-])(=O)=O HAEPBEMBOAIUPN-UHFFFAOYSA-L 0.000 description 1
239000007787 solid Substances 0.000 description 1
238000005063 solubilization Methods 0.000 description 1
230000007928 solubilization Effects 0.000 description 1
NHXLMOGPVYXJNR-ATOGVRKGSA-N somatostatin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CSSC[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2C3=CC=CC=C3NC=2)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N1)[C@@H](C)O)NC(=O)CNC(=O)[C@H](C)N)C(O)=O)=O)[C@H](O)C)C1=CC=CC=C1 NHXLMOGPVYXJNR-ATOGVRKGSA-N 0.000 description 1
229960000553 somatostatin Drugs 0.000 description 1
238000010186 staining Methods 0.000 description 1
239000007858 starting material Substances 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
230000009466 transformation Effects 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
239000012588 trypsin Substances 0.000 description 1
239000002753 trypsin inhibitor Substances 0.000 description 1
238000005406 washing Methods 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
239000011592 zinc chloride Substances 0.000 description 1
235000005074 zinc chloride Nutrition 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Gastroenterology & Hepatology (AREA)
Toxicology (AREA)
Plant Pathology (AREA)
Endocrinology (AREA)
Medicinal Chemistry (AREA)
Diabetes (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

FI920923A 1989-08-29 1992-02-28 Menetelmä fuusioproteiinien valmistamiseksi FI113183B (fi)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
US39987489A	1989-08-29	1989-08-29
US39987489		1989-08-29
US9004840		1990-08-28
PCT/US1990/004840 WO1991003550A1 (fr)	1989-08-29	1990-08-28	Proteines de fusion, leur preparation et utilisation

Publications (2)

Publication Number	Publication Date
FI920923A0 FI920923A0 (fi)	1992-02-28
FI113183B true FI113183B (fi)	2004-03-15

Family

ID=23581315

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
FI920923A FI113183B (fi)	1989-08-29	1992-02-28	Menetelmä fuusioproteiinien valmistamiseksi

Country Status (19)

Country	Link
EP (1)	EP0489780B1 (fr)
JP (1)	JP3043803B2 (fr)
KR (1)	KR0159786B1 (fr)
AT (1)	ATE173018T1 (fr)
AU (1)	AU638277B2 (fr)
CA (1)	CA2065146C (fr)
CY (1)	CY2168B1 (fr)
DE (1)	DE69032743T2 (fr)
DK (1)	DK0489780T3 (fr)
ES (1)	ES2124216T3 (fr)
FI (1)	FI113183B (fr)
GR (1)	GR1005153B (fr)
HU (1)	HU216069B (fr)
IE (1)	IE903120A1 (fr)
IL (1)	IL95495A (fr)
NO (1)	NO308667B1 (fr)
PT (1)	PT95111B (fr)
WO (1)	WO1991003550A1 (fr)
ZA (1)	ZA906839B (fr)

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DK0600372T3 (da) *	1992-12-02	1997-08-11	Hoechst Ag	Fremgangsmåde til fremstilling af proinsulin med korrekt forbundne cystinbroer.
DK0622376T3 (da) *	1993-04-27	2001-11-12	Hoechst Ag	Amorfe monosfæriske former af insulinderivater
WO1995009914A1 (fr) *	1993-10-05	1995-04-13	Asahi Glass Company Ltd.	Vecteur de multiclonage, vecteur d'expression, et production d'une proteine exogene au moyen dudit vecteur d'expression
US6001604A (en) *	1993-12-29	1999-12-14	Bio-Technology General Corp.	Refolding of proinsulins without addition of reducing agents
DE4405179A1 (de) *	1994-02-18	1995-08-24	Hoechst Ag	Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
US5683987A (en) *	1994-07-12	1997-11-04	The Board Of Regents Of The University Of Nebraska	Therapeutic oligonucleotides targeting the human MDR1 and MRP genes
DK0821006T3 (da)	1996-07-26	2004-08-16	Aventis Pharma Gmbh	Insulinderivater med öget zinkbinding
DE19735711C2 (de)	1997-08-18	2001-04-26	Aventis Pharma Gmbh	Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
DE19825447A1 (de)	1998-06-06	1999-12-09	Hoechst Marion Roussel De Gmbh	Neue Insulinanaloga mit erhöhter Zinkbildung
DE19915938A1 (de)	1999-04-09	2000-10-19	Aventis Pharma Gmbh	Herstellung von pankreatischer Procarboxypeptidase B, Isoformen und Muteinen davon und ihre Verwendung
DE19930676B4 (de)	1999-07-02	2006-01-19	Sanofi-Aventis Deutschland Gmbh	Verfahren zur Stabilisierung von Insulin, Insulinderivaten und/oder deren Vorläufer in komplexen Mischungen bei deren Lagerung in wäßrigen Lösungsmitteln
US7202059B2 (en)	2001-02-20	2007-04-10	Sanofi-Aventis Deutschland Gmbh	Fusion proteins capable of being secreted into a fermentation medium
US7638618B2 (en)	2001-02-20	2009-12-29	Sanofi-Aventis Deutschland Gmbh	Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
DE10235168A1 (de)	2002-08-01	2004-02-12	Aventis Pharma Deutschland Gmbh	Verfahren zur Reinigung von Preproinsulin
US20060035316A1 (en) *	2002-11-12	2006-02-16	Sang-Yong Lee	Plasmids expressing human insulin and the preparation method for human insuling thereby
DE102008025007A1 (de)	2008-05-24	2009-11-26	Sanofi-Aventis Deutschland Gmbh	Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
CN102007143B (zh)	2008-01-09	2015-08-26	塞诺菲-安万特德国有限公司	具有超延迟时效特征的新型胰岛素衍生物
DE102008003568A1 (de)	2008-01-09	2009-07-16	Sanofi-Aventis Deutschland Gmbh	Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
DE102008003566A1 (de)	2008-01-09	2009-07-16	Sanofi-Aventis Deutschland Gmbh	Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
EP2229406B1 (fr)	2008-01-09	2015-04-22	Sanofi-Aventis Deutschland GmbH	Nouveaux dérivés d'insuline à profil temporel/d'action extrêmement retardé
DE102008025008A1 (de)	2008-05-24	2009-11-26	Sanofi-Aventis Deutschland Gmbh	Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
CN102256618A (zh)	2008-10-17	2011-11-23	赛诺菲-安万特德国有限公司	胰岛素和glp-1激动剂的组合
PL2451437T3 (pl)	2009-07-06	2017-05-31	Sanofi-Aventis Deutschland Gmbh	Wodne preparaty insuliny zawierające metioninę
TR201809460T4 (tr)	2009-11-13	2018-07-23	Sanofi Aventis Deutschland	Bir GLP- 1-agonisti, bir insülin ve metiyonin içeren farmasötik bileşim.
NZ599847A (en)	2009-11-13	2013-09-27	Sanofi Aventis Deutschland	Pharmaceutical composition comprising a glp-1 agonist and methionine
WO2012028172A1 (fr)	2010-08-30	2012-03-08	Sanofi-Aventis Deutschland Gmbh	Utilisation de lixisenatide pour fabrication de médicament pour traitement du diabète sucré de type 2
DK2739646T3 (en)	2011-05-10	2018-02-26	Stefan Hermann	COMPREHENSIVE BUFFER SYSTEM FOR RENATURING HUMAN PROINSULIN OR PROINSULIN DERIVATIVES
US9821032B2 (en)	2011-05-13	2017-11-21	Sanofi-Aventis Deutschland Gmbh	Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
AR087693A1 (es)	2011-08-29	2014-04-09	Sanofi Aventis Deutschland	Combinacion farmaceutica para uso en el control glucemico en pacientes con diabetes de tipo 2
TWI559929B (en)	2011-09-01	2016-12-01	Sanofi Aventis Deutschland	Pharmaceutical composition for use in the treatment of a neurodegenerative disease
JP5858945B2 (ja)	2012-03-15	2016-02-10	アークレイ株式会社	酵素を用いた測定方法
PE20171622A1 (es)	2014-12-12	2017-11-02	Sanofi Aventis Deutschland	Formulacion de relacion fija de insulina glargina/lixisenatida
TWI748945B (zh)	2015-03-13	2021-12-11	德商賽諾菲阿凡提斯德意志有限公司	第２型糖尿病病患治療
TW201705975A (zh)	2015-03-18	2017-02-16	賽諾菲阿凡提斯德意志有限公司	第２型糖尿病病患之治療
JP6976254B2 (ja) *	2015-09-18	2021-12-08	バイオネットファーマゲゼルシャフトミットベシュレンクテルハフツングBioNet Pharma GmbH	Ｃｄ−ｒａｐ前駆体タンパク質の使用による、ｃｄ−ｒａｐの製造方法における発現およびフォールディングの改善
JP7460182B2 (ja) *	2019-02-19	2024-04-02	ザ、トラスティーズオブプリンストンユニバーシティ	フォールディングされたドメインおよびｒｎａ顆粒会合タンパク質ドメインの光制御オリゴマー化および相分離のためのシステムおよび方法

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US4652639A (en) *	1982-05-06	1987-03-24	Amgen	Manufacture and expression of structural genes
EP0195680A3 (fr) *	1985-03-21	1987-06-10	Immunex Corporation	Synthèse de protéines avec un peptide d'identification
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1990
- 1990-08-27 IL IL9549590A patent/IL95495A/en active IP Right Grant
- 1990-08-27 GR GR900100635A patent/GR1005153B/el not_active IP Right Cessation
- 1990-08-28 IE IE312090A patent/IE903120A1/en not_active IP Right Cessation
- 1990-08-28 DE DE69032743T patent/DE69032743T2/de not_active Expired - Lifetime
- 1990-08-28 DK DK90912715T patent/DK0489780T3/da active
- 1990-08-28 WO PCT/US1990/004840 patent/WO1991003550A1/fr not_active Ceased
- 1990-08-28 HU HUP9200674A patent/HU216069B/hu unknown
- 1990-08-28 CA CA002065146A patent/CA2065146C/fr not_active Expired - Lifetime
- 1990-08-28 ES ES90912715T patent/ES2124216T3/es not_active Expired - Lifetime
- 1990-08-28 KR KR1019920700456A patent/KR0159786B1/ko not_active Expired - Lifetime
- 1990-08-28 AU AU62872/90A patent/AU638277B2/en not_active Expired
- 1990-08-28 EP EP90912715A patent/EP0489780B1/fr not_active Expired - Lifetime
- 1990-08-28 JP JP2512297A patent/JP3043803B2/ja not_active Expired - Lifetime
- 1990-08-28 PT PT95111A patent/PT95111B/pt not_active IP Right Cessation
- 1990-08-28 AT AT90912715T patent/ATE173018T1/de not_active IP Right Cessation
- 1990-09-28 ZA ZA906839A patent/ZA906839B/xx unknown
1992
- 1992-02-27 NO NO920774A patent/NO308667B1/no not_active IP Right Cessation
- 1992-02-28 FI FI920923A patent/FI113183B/fi active
2000
- 2000-05-03 CY CY0000013A patent/CY2168B1/xx unknown

Also Published As

Publication number	Publication date
NO308667B1 (no)	2000-10-09
JPH05501799A (ja)	1993-04-08
FI920923A0 (fi)	1992-02-28
DK0489780T3 (da)	1999-07-19
GR900100635A (el)	1991-12-30
GR1005153B (el)	2006-03-13
AU6287290A (en)	1991-04-08
ZA906839B (en)	1991-06-26
DE69032743D1 (de)	1998-12-10
AU638277B2 (en)	1993-06-24
JP3043803B2 (ja)	2000-05-22
PT95111A (pt)	1991-05-22
EP0489780B1 (fr)	1998-11-04
ES2124216T3 (es)	1999-02-01
HU216069B (hu)	1999-04-28
WO1991003550A1 (fr)	1991-03-21
HK1012026A1 (en)	1999-07-23
CY2168B1 (en)	2002-08-23
DE69032743T2 (de)	1999-06-17
EP0489780A1 (fr)	1992-06-17
EP0489780A4 (en)	1992-08-12
HUT60327A (en)	1992-08-28
KR0159786B1 (ko)	1998-11-16
IE903120A1 (en)	1991-03-13
HU9200674D0 (en)	1992-08-28
PT95111B (pt)	1998-10-30
ATE173018T1 (de)	1998-11-15
IL95495A0 (en)	1991-06-30
CA2065146C (fr)	2002-07-23
NO920774D0 (no)	1992-02-27
CA2065146A1 (fr)	1991-03-01
NO920774L (no)	1992-04-28
IL95495A (en)	1996-10-16

Publication	Publication Date	Title
FI113183B (fi)	2004-03-15	Menetelmä fuusioproteiinien valmistamiseksi
US5358857A (en)	1994-10-25	Method of preparing fusion proteins
US5227293A (en)	1993-07-13	Fusion proteins, their preparation and use
AU615760B2 (en)	1991-10-10	Insulin analogues
FI102182B (fi)	1998-10-30	Menetelmä ihmisen insuliinianalogien valmistamiseksi
CA1340823C (fr)	1999-11-16	Precurseurs de l'insuline, ainsi qu un procede pour leur preparation et un procede pour la d'insulinee humaine
CA1340371C (fr)	1999-02-02	Methodes et produits pour faciliter l'expression microbienne de sequences d'adn
SK278576B6 (en)	1997-10-08	Expression vectors for increased production polypeptides, plasmides containing these vectors, host cells containing these plasmides, gained products, connecting methods and use
CA2006578A1 (fr)	1990-06-23	Analogues de l'insuline humaine
JP4624495B2 (ja)	2011-02-02	ヒト・インスリンの生成
JPH0759193B2 (ja)	1995-06-28	外来性蛋白質発現用クロ−ニングベクタ−
HK1006023A1 (en)	1999-02-05	Mini-proinsulin, its production and use
HK1006023B (en)	1999-02-05	Mini-proinsulin, its production and use
US4828988A (en)	1989-05-09	Hybrid polypeptides comprising somatocrinine and alpha1 -antitrypsin, method for their production from bacterial clones and use thereof for the production of somatocrinine
CA1338900C (fr)	1997-02-11	Vecteurs plasmidiques d'escherichia coli avec promoteur lipoproteinique et sequence shine-dalgarno modifiee
US6054291A (en)	2000-04-25	Expression vectors for enhanced production of polypeptides, plasmids containing the vectors, hosts containing the plasmids, products manufactured thereby and related methods
Mayne et al.	2018	Direct expression of human growth in Escherichia coli with the lipoprotein promoter
HK1012026B (en)	2000-04-28	Fusion proteins, their preparation and use
KR100381494B1 (ko)	2003-07-22	인간인슐린의제조방법
JPH08140679A (ja)	1996-06-04	微生物によるラットβ２−マイクログロブリン生産